(2S*,4S*)-4-[(E)-(2,2-Dimethylhydrazono)methyl]-6-methoxy-4-methyl-2-[(E)-styryl]-1,2,3,4-tetrahydroquinoline

The Povarov reaction of p-anisidine, cinnamaldehyde and methacrolein dimethylhydrazone afforded a 1,2,3,4-tetrahydroquinoline derivative bearing 2-styryl, 4-methyl and 4-dimethylhydrazono substituents in a fully diastereoselective fashion. This is the first example of the combination of a type I aza-vinylogous Povarov reaction and a type II vinylogous Povarov reaction in the same process.


Introduction
The 1,2,3,4-tetrahydroquinoline ring system is present in a broad variety of natural products and synthetic bioactive molecules [1,2] and can be considered as one of the most relevant simple heterocycles. Some important tetrahydroquinolines are summarized in Figure 1, including the alkaloids angustureine, virantmycin and aspoquinolone A, the cholesterol-lowering agent torcetrapib and the insecticidal, herbicidal and fungicidal aspernigerin ( Figure 1).
1a), stands out as one of the most studied methods [4,5]. Vinylogous Povarov reactions are also known, and have the advantage of generating tetrahydroquinolines with an olefin substituent either at C-4 (Type I, Scheme 1b) or C-2 (Type II, Scheme 1c). We have described [6,7] a type I aza-vinylogous Povarov reaction using α,β-unsaturated hydrazones as the dienophile component that has the advantage of simultaneously installing a quaternary stereocenter and functional group at C-4 (Scheme 1d).
In this context, we describe here the first example of a Povarov reaction that combines the features of a type I aza-vinylogous and a type II vinylogous Povarov reaction in a single transformation and allows the preparation of 2,4-difunctionalized 2-styryltetrahydroquinolines bearing a quaternary stereocenter at C-4 (Scheme 1e).

Results and Discussion
The doubly vinylogous Povarov reaction that we describe here is summarized in Scheme 2. The reaction between p-anisidine 1 and cinnamaldehyde 2 afforded the corresponding imine 3, which was treated in crude state with methacrolein dimethylhydrazone 4 in acetonitrile containing 10% indium trichloride as a Lewis acid catalyst, at room temperature, affording compound 5 as a single diastereomer in 40% overall yield (Scheme 2).

Scheme 2. Synthesis of compound 5.
The structure of compound 5 is consistent with a high-resolution mass measurement and with its IR, 1 H-NMR and 13 C-NMR spectral data, which were assigned with the aid of 2D-NMR experiments. Thus, the hydrazone group gave a CH=N stretching vibration at In this context, we describe here the first example of a Povarov reaction that combines the features of a type I aza-vinylogous and a type II vinylogous Povarov reaction in a single transformation and allows the preparation of 2,4-difunctionalized 2styryltetrahydroquinolines bearing a quaternary stereocenter at C-4 (Scheme 1e).

Results and Discussion
The doubly vinylogous Povarov reaction that we describe here is summarized in Scheme 2. The reaction between p-anisidine 1 and cinnamaldehyde 2 afforded the corresponding imine 3, which was treated in crude state with methacrolein dimethylhydrazone 4 in acetonitrile containing 10% indium trichloride as a Lewis acid catalyst, at room temperature, affording compound 5 as a single diastereomer in 40% overall yield (Scheme 2). 1a), stands out as one of the most studied methods [4,5]. Vinylogous Povarov reactions are also known, and have the advantage of generating tetrahydroquinolines with an olefin substituent either at C-4 (Type I, Scheme 1b) or C-2 (Type II, Scheme 1c). We have described [6,7] a type I aza-vinylogous Povarov reaction using α,β-unsaturated hydrazones as the dienophile component that has the advantage of simultaneously installing a quaternary stereocenter and functional group at C-4 (Scheme 1d).
In this context, we describe here the first example of a Povarov reaction that combines the features of a type I aza-vinylogous and a type II vinylogous Povarov reaction in a single transformation and allows the preparation of 2,4-difunctionalized 2-styryltetrahydroquinolines bearing a quaternary stereocenter at C-4 (Scheme 1e).

Results and Discussion
The doubly vinylogous Povarov reaction that we describe here is summarized in Scheme 2. The reaction between p-anisidine 1 and cinnamaldehyde 2 afforded the corresponding imine 3, which was treated in crude state with methacrolein dimethylhydrazone 4 in acetonitrile containing 10% indium trichloride as a Lewis acid catalyst, at room temperature, affording compound 5 as a single diastereomer in 40% overall yield (Scheme 2). The structure of compound 5 is consistent with a high-resolution mass measurement and with its IR, 1 H-NMR and 13 C-NMR spectral data, which were assigned with the aid of 2D-NMR experiments. Thus, the hydrazone group gave a CH=N stretching vibration at Scheme 2. Synthesis of compound 5.
The structure of compound 5 is consistent with a high-resolution mass measurement and with its IR, 1 H-NMR and 13 C-NMR spectral data, which were assigned with the aid of 2D-NMR experiments. Thus, the hydrazone group gave a CH=N stretching vibration at 1600 cm −1 in the IR spectrum, a 1 H-NMR signal in the 6.74-6.64 interval, which overlapped with other signals but is clearly visible in the HMQC experiment, and a 13 C-NMR signal at 145.3 ppm. One of the olefinic protons was clearly observed at 6.27 ppm as a doublet of doublets with J = 15.8 and 7.3 Hz, which allowed its assignment as H-α. The other proton corresponding to the olefin part of the styryl group (H-β) is part of the multiplet at 6.74-6.64, as revealed by the COSY experiment, and the H-C correlation experiments allowed the assignment of the olefin carbons to the CH signals at 132.4 (C-α) and 131.0 (C-β). The cis arrangement of the styryl and dimethylhydrazono substituents agrees with the literature precedents [6,7] and was unequivocally established by the observation of a NOE enhancement of the axial C-4 methyl substituent upon irradiation of the H-2 proton (Figure 2). The alternative trans isomer was not observed in the crude reaction product.
Molbank 2021, 2021, x FOR PEER REVIEW 3 of 5 1600 cm −1 in the IR spectrum, a 1 H-NMR signal in the 6.74-6.64 interval, which overlapped with other signals but is clearly visible in the HMQC experiment, and a 13 C-NMR signal at 145.3 ppm. One of the olefinic protons was clearly observed at 6.27 ppm as a doublet of doublets with J = 15.8 and 7.3 Hz, which allowed its assignment as H-α. The other proton corresponding to the olefin part of the styryl group (H-β) is part of the multiplet at 6.74-6.64, as revealed by the COSY experiment, and the H-C correlation experiments allowed the assignment of the olefin carbons to the CH signals at 132.4 (C-α) and 131.0 (C-β). The cis arrangement of the styryl and dimethylhydrazono substituents agrees with the literature precedents [6,7] and was unequivocally established by the observation of a NOE enhancement of the axial C-4 methyl substituent upon irradiation of the H-2 proton ( Figure  2). The alternative trans isomer was not observed in the crude reaction product.

Materials and Methods
General experimental information. All reagents (Sigma-Aldrich, Madrid, Spain; Fischer Chemical, Madrid, Spain; Alpha Aesar, Kändel, Germany) and solvents (Scharlau, Barcelona, Spain; Fischer Chemical, Madrid, Spain) were of commercial quality and were used as received. Reactions were monitored by thin layer chromatography on aluminum plates coated with silica gel and fluorescent indicator (Merck, Madrid, Spain). Infrared spectra were recorded with an Agilent Cary630 FTIR spectrophotometer (Madrid, Spain) working by attenuated total reflection (ATR), with a diamond accessory for solid and liquid samples. NMR spectroscopic data were recorded using a Bruker Avance 250 spectrometer (Bruker, Rivas-Vaciamadrid, Spain) operating at 250 MHz for 1 H-NMR and 63 MHz for 13 C-NMR maintained by the NMR facility of Universidad Complutense (CAI de Resonancia Magnética Nuclear, Madrid, Spain); chemical shifts are given in ppm and cou-

Materials and Methods
General experimental information. All reagents (Sigma-Aldrich, Madrid, Spain; Fischer Chemical, Madrid, Spain; Alpha Aesar, Kändel, Germany) and solvents (Scharlau, Barcelona, Spain; Fischer Chemical, Madrid, Spain) were of commercial quality and were used as received. Reactions were monitored by thin layer chromatography on aluminum plates coated with silica gel and fluorescent indicator (Merck, Madrid, Spain). Infrared spectra were recorded with an Agilent Cary630 FTIR spectrophotometer (Madrid, Spain) working by attenuated total reflection (ATR), with a diamond accessory for solid and liquid samples. NMR spectroscopic data were recorded using a Bruker Avance 250 spectrometer (Bruker, Rivas-Vaciamadrid, Spain) operating at 250 MHz for 1 H-NMR and 63 MHz for 13 C-NMR maintained by the NMR facility of Universidad Complutense (CAI de Resonancia Magnética Nuclear, Madrid, Spain); chemical shifts are given in ppm and coupling constants in Hertz. 1 H-and 13 C-NMR assignments were supported by 2D-NMR experiments and were aided by simulations performed with MestreNova and ChemDraw Pro. Copies of spectra and 2D-NMR experiments are provided in the Supporting Information. Time-of-flight mass spectrometric measurements were performed using a MALDI-TOF/TOF Bruker ULTRAFLEX (mass range: 300-150,000 u) at the CAI of Espectrometría de Masas, Universidad Complutense.

(E)-4-methoxy-N-((E)-3-phenylallylidene)aniline (3).
A solution of p-anisidine (1 mmol, 123 mg) and cinnamaldehyde (1 eq, 148 mg) in CH 2 Cl 2 (5 mL) was stirred vigorously in the presence of anhydrous Na 2 SO 4 (5 g) for 30 min and then the reaction mixture was filtered and the solvent evaporated under vacuum to afford 3 as a brown foam in quantitative yield. The crude was used in the following reaction without further purification. IR (