4,4″-Dichloro-4′-(2-thienyl)-2,2′:6′,2″-terpyridine

A new thiophene-substituted terpyridine derivative has been prepared and characterized. This ligand features a thiophene heterocycle (as an electrochemically polymerizable unit) as well as two chlorine atoms for further functionalization.


Introduction
Terpyridine (terpy) derivatives (especially 2,2 :6 ,2"-terpyridines) and their complexes have been widely studied [1]. In fact, an important number of terpyridine-based compounds can be prepared by varying the substitution pattern onto the terpyridine scaffold and/or the nature of the complexed metal. Terpyridines and their complexes have found a vast range of applications such as sensitizers in solar cells [2], catalysts [3], materials for water treatment [4], electrochromic materials [5], or as chromophores [6], just to name a few. Terpyridine complexes that are substituted with a thiophene ring are interesting compounds because they also find many possible applications in material science [7] or medicinal chemistry and biology [8,9], for example. Especially, the thiophene heterocycle allows for the formation of polymeric materials through electrochemical polymerization. The so obtained terpyridine-containing materials have shown potential uses as sensors [10] or as electrochromic materials [11], for example. Therefore, the synthesis of new terpy derivatives that bear a pendant thiophene ring is still of interest. This article follows our previous work on furan-containing terpy [12] and describes the synthesis and characterization of 4,4"-dichloro-4 -(2-thienyl)-2,2 :6 ,2"-terpyridine (1) (Figure 1). This ligand not only features a thiophene ring but also possesses two chlorine atoms on the outer pyridine rings, which allow for future functionalization of this terpyridine.

Introduction
Terpyridine (terpy) derivatives (especially 2,2':6',2''-terpyridines) and their complexes have been widely studied [1]. In fact, an important number of terpyridine-based compounds can be prepared by varying the substitution pattern onto the terpyridine scaffold and/or the nature of the complexed metal. Terpyridines and their complexes have found a vast range of applications such as sensitizers in solar cells [2], catalysts [3], materials for water treatment [4], electrochromic materials [5], or as chromophores [6], just to name a few. Terpyridine complexes that are substituted with a thiophene ring are interesting compounds because they also find many possible applications in material science [7] or medicinal chemistry and biology [8,9], for example. Especially, the thiophene heterocycle allows for the formation of polymeric materials through electrochemical polymerization. The so obtained terpyridine-containing materials have shown potential uses as sensors [10] or as electrochromic materials [11], for example. Therefore, the synthesis of new terpy derivatives that bear a pendant thiophene ring is still of interest. This article follows our previous work on furancontaining terpy [12] and describes the synthesis and characterization of 4,4′′-dichloro-4′-(2-thienyl)-2,2′:6′,2′′-terpyridine (1) (Figure 1). This ligand not only features a thiophene ring but also possesses two chlorine atoms on the outer pyridine rings, which allow for future functionalization of this terpyridine.

Results and Discussion
Amongst all methods available for the preparation of terpyridine derivatives [13][14][15], the one from Wang and Hanan [16] was selected, although sometimes this method fails in producing Amongst all methods available for the preparation of terpyridine derivatives [13][14][15], the one from Wang and Hanan [16] was selected, although sometimes this method fails in producing terpyridine derivatives [17]. Thus 1 was simply prepared by mixing 4-chloro-2-acetylpyridine [18], thiophene-2-carboxaldehyde, and potassium hydroxide in ethanol and aqueous ammonia ( Figure 2). Crude 1 precipitated from the reaction mixture and was isolated by simple filtration. At this point, the material collected was sufficiently pure (>98% by quantitative NMR [19]) to be used for further experiments (e.g., preparation of metal complexes) without purification.
The identity of compound 1 was confirmed by different analytical techniques. Firstly, NMR spectra ( 1 H and 13 C) agreed with the chemical structure. Especially, the singlet for proton 3′ and 5′ was clearly seen at δ = 8.72 ppm. Furthermore, signals for protons 3, 3′′, 6, and 6′′ were merged into a multiplet while protons 5 and 5′′ appeared as a doublet of doublet ( Figure 3). Such an 1 H NMR pattern has been already observed for 4,4′′-dichloro-2,2′:6′,2′′-terpyridine [20]. Additionally, the formation of the title compound was confirmed by HR-MS ( Figure S3) since the [M + H] + ion peak, as well as the isotopic distribution, corresponded to molecular formula (C19H12Cl2N3S + ). In fact, the experimental recorded spectrum was in good agreement with the simulated one ( Figure 4). Crude 1 precipitated from the reaction mixture and was isolated by simple filtration. At this point, the material collected was sufficiently pure (>98% by quantitative NMR [19]) to be used for further experiments (e.g., preparation of metal complexes) without purification.
The identity of compound 1 was confirmed by different analytical techniques. Firstly, NMR spectra ( 1 H and 13 C) agreed with the chemical structure. Especially, the singlet for proton 3 and 5 was clearly seen at δ = 8.72 ppm. Furthermore, signals for protons 3, 3", 6, and 6" were merged into a multiplet while protons 5 and 5" appeared as a doublet of doublet ( Figure 3). Such an 1 H NMR pattern has been already observed for 4,4"-dichloro-2,2 :6 ,2"-terpyridine [20]. Amongst all methods available for the preparation of terpyridine derivatives [13][14][15], the one from Wang and Hanan [16] was selected, although sometimes this method fails in producing terpyridine derivatives [17]. Thus 1 was simply prepared by mixing 4-chloro-2-acetylpyridine [18], thiophene-2-carboxaldehyde, and potassium hydroxide in ethanol and aqueous ammonia (Figure 2). Crude 1 precipitated from the reaction mixture and was isolated by simple filtration. At this point, the material collected was sufficiently pure (>98% by quantitative NMR [19]) to be used for further experiments (e.g., preparation of metal complexes) without purification.
The identity of compound 1 was confirmed by different analytical techniques. Firstly, NMR spectra ( 1 H and 13 C) agreed with the chemical structure. Especially, the singlet for proton 3′ and 5′ was clearly seen at δ = 8.72 ppm. Furthermore, signals for protons 3, 3′′, 6, and 6′′ were merged into a multiplet while protons 5 and 5′′ appeared as a doublet of doublet ( Figure 3). Such an 1 H NMR pattern has been already observed for 4,4′′-dichloro-2,2′:6′,2′′-terpyridine [20]. Additionally, the formation of the title compound was confirmed by HR-MS ( Figure S3) since the [M + H] + ion peak, as well as the isotopic distribution, corresponded to molecular formula (C19H12Cl2N3S + ). In fact, the experimental recorded spectrum was in good agreement with the simulated one ( Figure 4). Additionally, the formation of the title compound was confirmed by HR-MS ( Figure S3) since the [M + H] + ion peak, as well as the isotopic distribution, corresponded to molecular formula (C 19 H 12 Cl 2 N 3 S + ). In fact, the experimental recorded spectrum was in good agreement with the simulated one ( Figure 4).

Materials and Methods
The 4-chloropicolinonitrile was purchased from ABCR Gmbh (Karlsruhe, Germany) and used as received. Methylmagnesium chloride (20% solution in THF) was purchased from Merck Schuchardt (Hohenbrunn, Germany). This Grignard solution was titrated prior to use with salicylaldehyde phenylhydrazone according to literature [21] and the measured concentration was 2.9 M. All other chemicals were purchased from ACROS organics (Geel, Belgium). Anhydrous THF was obtained from a solvent purification system (Innovative Technology, Amesbury, MA, USA).The 4-chloro-2-acetylpyridine was prepared according to the literature [18]. 1 H and 13 C NMR spectra were recorded on a Brucker AC 400 (Bruker, Wissembourg, France) at 400 and 100 MHz, respectively, using CDCl3 as a solvent. Melting point was recorded with a Stuart SMP 10 melting point apparatus (Bibby Sterilin, Stone, UK) and was uncorrected. Elemental analysis was performed at Service d'Analyses Elementaires, UMR 7565 CNRS, Vandoeuvre-les-Nancy, France. HR-MS was recorded at Sayence SATT, Dijon, France.

Preparation of 4-Chloro-2-acetylpyridine
To a solution of methylmagnesium chloride (50 mL; 0.145 mol, excess) at 0 °C under argon was added dropwise a solution of 4-chloropicolinonitrile (5.00 g; 0.036 mol) in anhydrous THF (120 mL). The reaction mixture was stirred at room temperature for 6 h. The solution was then poured cautiously onto a saturated ammonium chloride aqueous solution (120 mL) at 0 °C, and the pH of the solution was adjusted to 1 with concentrated hydrochloric acid. The resulting solution was stirred at room temperature overnight and then neutralized with 25% aqueous ammonia. The aqueous solution was extracted with ethyl acetate (6 × 50 mL). Organic layers were combined, washed with brine (2 × 100 mL), dried over sodium sulphate, and concentrated. The crude product was purified by flash chromatography with cyclohexane/ethylacetate as eluent (100:0 to 95:5). Pure product was obtained as a pale-yellow oil (2.58 g; 50%). Spectroscopic data agreed with those reported in the literature [18].

Materials and Methods
The 4-chloropicolinonitrile was purchased from ABCR Gmbh (Karlsruhe, Germany) and used as received. Methylmagnesium chloride (20% solution in THF) was purchased from Merck Schuchardt (Hohenbrunn, Germany). This Grignard solution was titrated prior to use with salicylaldehyde phenylhydrazone according to literature [21] and the measured concentration was 2.9 M. All other chemicals were purchased from ACROS organics (Geel, Belgium). Anhydrous THF was obtained from a solvent purification system (Innovative Technology, Amesbury, MA, USA). The 4-chloro-2-acetylpyridine was prepared according to the literature [18]. 1 H and 13 C NMR spectra were recorded on a Brucker AC 400 (Bruker, Wissembourg, France) at 400 and 100 MHz, respectively, using CDCl 3 as a solvent. Melting point was recorded with a Stuart SMP 10 melting point apparatus (Bibby Sterilin, Stone, UK) and was uncorrected. Elemental analysis was performed at Service d'Analyses Elementaires, UMR 7565 CNRS, Vandoeuvre-les-Nancy, France. HR-MS was recorded at Sayence SATT, Dijon, France.

Preparation of 4-Chloro-2-acetylpyridine
To a solution of methylmagnesium chloride (50 mL; 0.145 mol, excess) at 0 • C under argon was added dropwise a solution of 4-chloropicolinonitrile (5.00 g; 0.036 mol) in anhydrous THF (120 mL). The reaction mixture was stirred at room temperature for 6 h. The solution was then poured cautiously onto a saturated ammonium chloride aqueous solution (120 mL) at 0 • C, and the pH of the solution was adjusted to 1 with concentrated hydrochloric acid. The resulting solution was stirred at room temperature overnight and then neutralized with 25% aqueous ammonia. The aqueous solution was extracted with ethyl acetate (6 × 50 mL). Organic layers were combined, washed with brine (2 × 100 mL), dried over sodium sulphate, and concentrated. The crude product was purified by flash chromatography with cyclohexane/ethylacetate as eluent (100:0 to 95:5). Pure product was obtained as a pale-yellow oil (2.58 g; 50%). Spectroscopic data agreed with those reported in the literature [18].
Author Contributions: J.H. conceived and carried out the experiments, analyzed data, and prepared the manuscript. L.G. analyzed data and contributed to manuscript preparation.

Conflicts of Interest:
The authors declare no conflict of interest.