Ethyl 4-( 2-fluorophenyl )-6-methyl-2-thioxo-1-( p-tolyl )-1 , 2 , 3 , 4-tetrahydropyrimidine-5-carboxylate

The Biginelli reaction is a highly versatile reaction that leads to dihydropyrimidinones/ thiones. This scaffold is reported as being a privileged structure due to its ability to interact with biological targets. Synthesis of ethyl 4-(2-fluorophenyl)-6-methyl-2-thioxo-1-(p-tolyl)-1,2,3,4tetrahydropyrimidine-5-carboxylate was achieved through the Biginelli reaction using a functionalized thiourea. In silico studies demonstrated that the compound title showed good potential for interacting with ecto-5’-nucleotidase, which has been considered as a target in designs for anti-cancer drugs.


Introduction
The Biginelli reaction involves the acid-catalyzed multicomponent synthesis of 3,4dihydropyrimidin-2(1H)-ones(thiones) (DHPMs) [1].Thus, this reaction is a powerful tool for the fast and easy generation of libraries with great structural diversity [2].DHPMs are an example of privileged scaffolds due to their interaction with numerous biological targets [3].
It has been recently identified that N1 substituted Biginelli compounds were able to interact with Eg5, a protein involved in chromosome separation during the cell cycle [4].Another target for this heterocycle class is the enzyme ecto-5 -nucleotidase (5 -NT or CD73), a protein involved in the hydrolysis of adenosine monophosphate (AMP) to adenosine, a molecule that increases cancer growth through immune system suppression [5].DHPM LaSOM 63 was identified as a prototype for the development of an ecto-5 -nucleotidase inhibitor.LaSOM 63 was active against glioma cells and inhibited the phosphate liberation from cells treated with AMP [6].In this context, in continuation with our studies focused on exploring the possibility of finding a DHPM ecto-5 -nucleotidase inhibitor, a new N1-aryl substituted DHPM was designed and synthesized.

Results and Discussion
A virtual library containing 528 compounds was constructed from dihydropyrimidinone scaffolds.For this purpose, 22 thioureas and 24 aldehydes were included in the SMILIB program [7].SMILIB is able to construct very large combinatorial compound libraries in the SMILES format.
The structural elucidation of the compound ( 6) was made based on spectroscopic data, and the results are displayed in the experimental section and in the electronic supporting information.From the 1 H-NMR spectra (Figure S1), one broad singlet of the NH-3 proton of the DHPM core appeared at 7.53 ppm.Aromatic hydrogens of the two rings produced the next group of signals at 6.91-7.34ppm.The benzylic hydrogen produced a signal at 5.72 ppm, while the singlet of allylic CH 3 resonated at 2.18 ppm.The signals that appeared as a triplet and quartet at 1.14 and 4.08 ppm, respectively, corresponded to the H 3 CCH 2 system.The methyl group linked to the aromatic ring at N1 produced a signal at 2.40 ppm.

Chemical Analysis
All the chemicals were purchased as reagent grade and used without further purification.Melting points were determined on a Fisatom 431 apparatus, and were uncorrected.Nuclear magnetic resonance spectra of carbon and proton were recorded in a Bruker Ascend NMR with standard pulse sequences operating at 400 MHz for 1 H-NMR and 100 MHz for 13 C-NMR using CDCl3 as a solvent.FT-IR spectra was obtained in a Perkin Elmer spectrometer, opening in ATR mode.In the APT 13 C-NMR spectrum (Figure S2), the most representative signals were the methyl carbons at 14.0 and 18.6 ppm, the methyne carbon at 49.6 ppm, and the methylene carbon at 60.6 ppm.The ester carbonylic carbon produced a signal at 165.5 ppm, while the most downfield signals, which appeared at 178.8 ppm, corresponded to the quaternary carbon of the C=S bond carbonyl, which are situated between the two nitrogens.In addition, the ipso, ortho, meta, and para couplings between 19 F-13 C were identified.The expansion of the aromatic region allowed for the calculation of the coupling 19 F-13 C constants and the assignment of the aromatic carbons of the ring linked to position 4 of the DHPM core.

Chemical Analysis
All the chemicals were purchased as reagent grade and used without further purification.Melting points were determined on a Fisatom 431 apparatus, and were uncorrected.Nuclear resonance spectra of carbon and proton were recorded in a Bruker Ascend NMR with standard pulse sequences operating at 400 MHz for 1 H-NMR and 100 MHz for 13 C-NMR using CDCl 3 as a solvent.FT-IR spectra was obtained in a Perkin Elmer spectrometer, opening in ATR mode.

Synthesis of 1-(4-Methylphenyl)thiourea
For a solution of ammonium thiocyanate (1.1.equiv) in dry acetone at room temperature, benzoyl chloride (1 equiv) was added and the solution was heated at 70 • C for 15 min.Following this, p-toluidine (1 equiv) in acetone was dropwise added and heating of the solution was maintained for 30 min.The reactional medium was poured into water at room temperature and the precipitate was filtered and submitted to alkaline hydrolysis in NaOH 2.50 M, 90 • C for 20 min.Following this, the pH of the system was adjusted to 2 with HCl 37%, and 8 with NH 4 OH 28%.The product was filtered and presented a high level of purity A mixture of ethyl acetoacetate (1 equiv), 1-(4-methylphenyl)thiourea (1 equiv) and a substituted benzaldehyde (1 equiv) were solubilized in DMF under ultrasound for 1 h.TMSCl (6 equiv) was then added dropwise and the mixture was stirred at room temperature for 72 h.After the mixture was poured over three volumes of water, it was submitted to ultrasound for one hour and the precipitate obtained was filtered and washed with water.The crude isolate was recrystallized from ethanol.