Thymol, a Monoterpenoid within Polymeric Iodophor Formulations and Their Antimicrobial Activities

Antimicrobial resistance (AMR) poses an emanating threat to humanity’s future. The effectiveness of commonly used antibiotics against microbial infections is declining at an alarming rate. As a result, morbidity and mortality rates are soaring, particularly among immunocompromised populations. Exploring alternative solutions, such as medicinal plants and iodine, shows promise in combating resistant pathogens. Such antimicrobials could effectively inhibit microbial proliferation through synergistic combinations. In our study, we prepared a formulation consisting of Aloe barbadensis Miller (AV), Thymol, iodine (I2), and polyvinylpyrrolidone (PVP). Various analytical methods including SEM/EDS, UV-vis, Raman, FTIR, and XRD were carried out to verify the purity, composition, and morphology of AV-PVP-Thymol-I2. We evaluated the inhibitory effects of this formulation against 10 selected reference strains using impregnated sterile discs, surgical sutures, gauze bandages, surgical face masks, and KN95 masks. The antimicrobial properties of AV-PVP-Thymol-I2 were assessed through disc diffusion methods against 10 reference strains in comparison with two common antibiotics. The 25-month-old formulation exhibited slightly lower inhibitory zones, indicating changes in the sustained-iodine-release reservoir. Our findings confirm AV-PVP-Thymol-I2 as a potent antifungal and antibacterial agent against the reference strains, demonstrating particularly strong inhibitory action on surgical sutures, cotton bandages, and face masks. These results enable the potential use of the formulation AV-PVP-Thymol-I2 as a promising antimicrobial agent against wound infections and as a spray-on contact-killing agent.

Our title formulation AV-PVP-Thymol-I 2 was verified as a strong antifungal agent against C. albicans WDCM 00054 Vitroids.Among the most susceptible Gram-positive bacteria were S. aureus ATCC 25923, B. subtilis WDCM0003, and E. faecalis ATCC 29212.Moderate to intermediate inhibitory action was observed against the Gram-negative microorganisms K. pneumonia WDCM00097 Vitroids, E. coli WDCM 00013 Vitroids, and P. aeruginosa WDCM 00026 Vitroids depending on the impregnated material.Accordingly, the highest inhibition zones were recorded where layers of surgical and KN95 face masks, as well as gauze bandages, were used.These results indicate the potential applications of the title compound AV-PVP-Thymol-I 2 to prevent or treat infections and act as a contact-killing agent on inanimate surfaces.Further in vivo experiments and cytotoxicity studies are needed to verify the suggested applications.

Morphological Examination and Elemental Composition of AV-PVP-Thymol-I2
The composition and morphology of AV-PVP-Thymol-I2 were verified by energy-dispersive X-ray spectroscopic (EDS) and Electron Microscope (SEM) analysis, respectively (Figure 1). Figure 1a reveals a smooth and finely homogenous morphology of AV-PVP-Thymol-I2 with few small, rounded structures similar to AV-PVP-Thyme-I2 (Figure 1a, Supplementary File S1) [42].The EDS in Figure 1b shows the presence of carbon (42%), oxygen (27%), iodine (3%), Cu (1.8%), and further elements originating from AV. Aluminium (24%) appears due to the sample holder [58].Gold is used to coat the formulation, and therefore, it is seen at a low intensity in the EDS.Accordingly, the purity of the title formulation AV-PVP-Thymol-I2 is verified by EDS.
We coated absorbable, braided polyglycolic acid (PGA) sutures, gauze bandages, and KN95 and sterile surgical face masks with the title formulation AV-PVP-Thymol-I2 at a concentration of 11 µg/mL.The impregnated material was subsequently analyzed by SEM and EDS.Analyses of plain and dip-coated surgical PGA sutures are depicted in Figure 2. Figure 1a reveals a smooth and finely homogenous morphology of AV-PVP-Thymol-I 2 with few small, rounded structures similar to AV-PVP-Thyme-I 2 (Figure 1a, Supplementary File S1) [42].The EDS in Figure 1b shows the presence of carbon (42%), oxygen (27%), iodine (3%), Cu (1.8%), and further elements originating from AV. Aluminium (24%) appears due to the sample holder [58].Gold is used to coat the formulation, and therefore, it is seen at a low intensity in the EDS.Accordingly, the purity of the title formulation AV-PVP-Thymol-I 2 is verified by EDS.
We coated absorbable, braided polyglycolic acid (PGA) sutures, gauze bandages, and KN95 and sterile surgical face masks with the title formulation AV-PVP-Thymol-I 2 at a concentration of 11 µg/mL.The impregnated material was subsequently analyzed by SEM and EDS.Analyses of plain and dip-coated surgical PGA sutures are depicted in Figure 2.

Morphological Examination and Elemental Composition of AV-PVP-Thymol-I2
The composition and morphology of AV-PVP-Thymol-I2 were verified by energy-dispersive X-ray spectroscopic (EDS) and Electron Microscope (SEM) analysis, respectively (Figure 1). Figure 1a reveals a smooth and finely homogenous morphology of AV-PVP-Thymol-I2 with few small, rounded structures similar to AV-PVP-Thyme-I2 (Figure 1a, Supplementary File S1) [42].The EDS in Figure 1b shows the presence of carbon (42%), oxygen (27%), iodine (3%), Cu (1.8%), and further elements originating from AV. Aluminium (24%) appears due to the sample holder [58].Gold is used to coat the formulation, and therefore, it is seen at a low intensity in the EDS.Accordingly, the purity of the title formulation AV-PVP-Thymol-I2 is verified by EDS.
We coated absorbable, braided polyglycolic acid (PGA) sutures, gauze bandages, and KN95 and sterile surgical face masks with the title formulation AV-PVP-Thymol-I2 at a concentration of 11 µg/mL.The impregnated material was subsequently analyzed by SEM and EDS.Analyses of plain and dip-coated surgical PGA sutures are depicted in Figure 2. Coating a plain PGA suture (Figure 2a) with the title formulation AV-PVP-Thymol-I 2 leads to a homogenous, smooth PGA surface in the SEM (Figure 2b) [45].Consequently, such coated sutures can alternatively be utilized to prevent surgical-site infections due to their homogenous, antimicrobial coating [116,117].The EDS analysis depicts carbon (53%), oxygen (40%), iodine (2%), copper (4%), and chlorine (0.3%), similar to the results of AV-PVP-Thyme-I 2 (Figure 2c, Supplementary File S2) [42].Copper and chlorine can be attributed to the AV components and even the suture itself, while the EDS verifies the purity of the title formulation.
During any epidemic or pandemic, personal protection equipment is pivotal for mitigating the spread of diseases and infectious agents [8,[118][119][120][121][122][123][124].Face masks prevent upper respiratory tract infections up to a certain level depending on their quality and the pathogen characteristics [8,[118][119][120][121][122][123][124].The most common ones are single-use, plain surgical face masks and KN95 masks.However, disposable face masks cause environmental pollution and are not a sustainable solution for future pandemics [124].Dip-coating face masks with antimicrobials could increase their protective effects and enable their re-use [42,[118][119][120][121][122][123].Consequently, re-using would remove the burden on environment and public spending and presents a sustainable solution for the planet and the safety of the global population.
The SEM and EDS analysis of coated surgical facemasks confirms the purity and homogenous, smooth appearance of AV-PVP-Thymol-I 2 (Figure 3, Supplementary File S3).
such coated sutures can alternatively be utilized to prevent surgical-site infections due to their homogenous, antimicrobial coating [116,117].The EDS analysis depicts carbon (53%), oxygen (40%), iodine (2%), copper (4%), and chlorine (0.3%), similar to the results of AV-PVP-Thyme-I2 (Figure 2c, Supplementary File S2) [42].Copper and chlorine can be attributed to the AV components and even the suture itself, while the EDS verifies the purity of the title formulation.
During any epidemic or pandemic, personal protection equipment is pivotal for mitigating the spread of diseases and infectious agents [8,[118][119][120][121][122][123][124].Face masks prevent upper respiratory tract infections up to a certain level depending on their quality and the pathogen characteristics [8,[118][119][120][121][122][123][124].The most common ones are single-use, plain surgical face masks and KN95 masks.However, disposable face masks cause environmental pollution and are not a sustainable solution for future pandemics [124].Dip-coating face masks with antimicrobials could increase their protective effects and enable their re-use [42,[118][119][120][121][122][123].Consequently, re-using would remove the burden on environment and public spending and presents a sustainable solution for the planet and the safety of the global population.
The SEM and EDS analysis of coated surgical facemasks confirms the purity and homogenous, smooth appearance of AV-PVP-Thymol-I2 (Figure 3, Supplementary File S3).The utilized surgical face masks comprised a white (towards the face) and a blue layer (away from face), which were separately analyzed by SEM and EDS.The white layer is a very dense network of fine, white fibres (Figure 3a).Coating the material with AV-PVP-Thymol-I2 results in a denser network of smoothly covered fibres (Figure 3b).The EDS of the white layer exhibits carbon (84%), oxygen (8%), iodine (4%), and copper (3%) The utilized surgical face masks comprised a white (towards the face) and a blue layer (away from face), which were separately analyzed by SEM and EDS.The white layer is a very dense network of fine, white fibres (Figure 3a).Coating the material with AV-PVP-Thymol-I 2 results in a denser network of smoothly covered fibres (Figure 3b).The EDS of the white layer exhibits carbon (84%), oxygen (8%), iodine (4%), and copper (3%) (Figure 3c).Chlorine and potassium are almost irrelevant and are related to the face mask material or AV gel.Accordingly, the purity of AV-PVP-Thymol-I 2 is therefore confirmed by the absence of any other unrelated element (Figure 3c).The blue layer consists of darker and thicker fibres with a nodular, uneven surface texture (Figure 3d).The EDS analysis depicts carbon (87%), oxygen (7%), copper (4%), and iodine (3%), and therefore verifies the purity of the formulation on blue face mask tissues (Supplementary File S1).After coating the blue face mask layer, the SEM in Figure 3d presents homogenously coated, smooth fibres in contrast to the previously rough-grained fibers in Figure 3d.A detailed picture of the coating formulation AV-PVP-Thymol-I 2 is seen in Figure 3e.The smooth surface consists of very small, almost nanoparticle-sized, smooth, round-shaped moieties like in the previous formulation, AV-PVP-Thyme-I 2 [42].Apparently, both formulations consist of similar semi-crystalline, small, square-and spherical-shaped compositions, which homogenously coats materials.Coating sterile surgical face masks with our homogenous, antimicrobial title formulation could mitigate airborne infections in the upper respiratory tract [42,[118][119][120][121][122][123].
the purity of the formulation on blue face mask tissues (Supplementary File S1).After coating the blue face mask layer, the SEM in Figure 3d presents homogenously coated, smooth fibres in contrast to the previously rough-grained fibers in Figure 3d.A detailed picture of the coating formulation AV-PVP-Thymol-I2 is seen in Figure 3e.The smooth surface consists of very small, almost nanoparticle-sized, smooth, round-shaped moieties like in the previous formulation, AV-PVP-Thyme-I2 [42].Apparently, both formulations consist of similar semi-crystalline, small, square-and spherical-shaped compositions, which homogenously coats materials.Coating sterile surgical face masks with our homogenous, antimicrobial title formulation could mitigate airborne infections in the upper respiratory tract [42,[118][119][120][121][122][123].
The uniform, fine, and even coating of face masks and cotton bandages, as well as braided PGA sutures, unlocks the potential utilization of our title compound AV-PVP-Thymol-I2 as a contact-killing agent on those materials, as well as antimicrobial dressings.The title formulation AV-PVP-Thymol-I 2 uniformly coats the cotton gauze bandage (Figure 4).In the detailed view, AV-PVP-Thyme-I 2 and the title compound reveal the same semi-crystalline, smooth pattern of nanoscale, almost spherical, even entities like in Figure 3f [42].EDS of the title formulation AV-PVP-Thymol-I 2 on the cotton bandage detects carbon (54%), oxygen (38%), copper (6%), and iodine (2%).Chlorine originates from AV or the bandage, while gold is due to coating the sample with Au.In comparison, Zhan et al. coated cotton fabric with Thymol for antimicrobial purposes [76].Their SEM also showed a homogenous, smooth pattern of coating on the cotton fabric in agreement with our results in Figure 4b.
The uniform, fine, and even coating of face masks and cotton bandages, as well as braided PGA sutures, unlocks the potential utilization of our title compound AV-PVP-Thymol-I 2 as a contact-killing agent on those materials, as well as antimicrobial dressings.
The absorptions of the components Thymol, PVP, AV, and iodine units overlap with each other, which consequently results in broad absorption bands [42,43,45,46,48].Therefore, Thymol and AV bio-compounds are significantly overshadowed by the strongerintensity absorptions of PVP and iodine moieties, posing a challenge for spectral analysis [42,43,45,46,48].Nevertheless, comparing these spectra with pure Thymol and the previously studied AV-PVP-Thyme-I 2 allows for better predictions regarding the composition of the title formulation (Table 2) [42].Thymol appears clearly around 282 nm, which is red-shifted towards higher wavelengths in comparison to pure Thymol at 277 nm and 279 nm in previous reports [42,71].This bathochromic shift indicates an increase in conjugated systems and a solvent effect leading to a reduced energy gap and less-encapsulated, free Thymol molecules within our title compound.The increased absorption intensity of the title compound compared (red curve) to pure Thymol (green curve) also confirms less-encapsulated Thymol molecules with increased availability of pi-electrons and reduced hydrogen bonding (Figure 6a).
After storing AV-PVP-Thymol-I 2 for 25 months (light blue curve), the UV-vis spectrum shows almost identical absorptions without any noticeable changes except higher absorption intensities (Figure 6, Table 2).This suggests that the triiodide absorption intensities, as well as their increased band broadness, indicate not only enhanced hydrogen bonding within the formulation but also a prevalence of symmetrical [I-I-I − ] over asymmetrical triiodide [I-I . ... I − ] moieties.This assumption is further supported by the previous Raman spectrum, revealing a strong absorption peak at 112 cm −1 for "smart" triiodides and a broad, weak absorption band at 141 and 145 cm −1 for asymmetric [I-I . ... I − ] units (Figure 5, Table 1) [42,43,45,46,48,51].Additionally, there are remarkable increases in absorption intensities and bathochromic red shifts related to Thymol and aloin in the 25-month-old sample.These consist of shifts for aloin from 206 to 209 nm.Thymol shows red shifts from 203 to 205 nm, 209 to 212 nm, 218 to 220 nm, and 222 to 223 nm (Figure 6, Table 2).Such red shifts towards the longer-wavelength region suggest a release of Thymol from the polymeric PVP complexation in exchange of triiodide moieties.Interestingly, there is a sharp reduction in iodine absorption at 203 nm in the 25-month-old sample.This observation indicates the loss of active iodine molecules from the PVP during storage, although the colour of the sample did not noticeably change during the 25 months.Consequently, reduced inhibitory action against the tested pathogens is expected.These assumptions are verified in the antimicrobial testing results depicted below in the coming sections of this study.
Initially, Thymol and the bioactive constituents of AV could potentially compete with triiodides for complexation by PVP via hydrogen bonding.Certain plant components from AV may disrupt this process by forming hydrogen bonds in place of triiodide ions, consequently facilitating their release from PVP.Such interference could compromise the antimicrobial efficacy of the formulation under investigation.Notably, the antimicrobial test results of the fresh title compound exhibit remarkable efficacy.However, lower inhibitory activity is observed after 25 months of storage in comparison to the long-term study of the formulation AV-PVP-Thyme-I 2 [42].The latter exhibited even higher inhibition zones after 18 months compared to the initial fresh formulation AV-PVP-Thyme-I 2 [42].As a result, the plant-based formulation AV-PVP-Thyme-I 2 derived from an ethanolic Thyme extract has increased antimicrobial activity due to the synergy of the spectrum of bio-compounds available naturally within the macerated sample [42].Those bio-compounds stabilize the polymeric iodophor and prolong, and even enhance, the sustained-release reservoir.This happens through protecting the iodine molecules captured within the PVP, inhibiting their release and the decomposition of the formulation AV-PVP-Thyme-I 2 [42].This synergetic, protective, and sustaining effect is missing in the title formulation AV-PVP-Thymol-I 2 because the natural biocomponents of Thyme are missing [42].Therefore, the sustained-release reservoir is not protected and starts to slowly release the active species iodine over a period of 25 months.Consequently, this results in reduced antimicrobial action after 25 months of storage before being exposed to any microorganism.However, the antimicrobial testing on the 10 pathogens still shows good to intermediate results.
In summary, the presence of Thymol and AV bio-compounds in the AV-PVP-Thymol-I 2 formulation does not diminish its inhibitory effectiveness in the fresh sample.After prolonged storage of 25 months, there is reduced antimicrobial action.PVP effectively shields triiodide ions within the formulation through hydrogen bonding, serving as a sustained-release reservoir by impeding the decomposition of triiodide ions.In contrast, without this protective mechanism, iodine release would occur, leading to the discoloration of AV-PVP-Thymol-I 2 and diminished antimicrobial efficacy over time.However, our findings from UV spectral analysis and antimicrobial testing confirm the absence of discoloration, with a slight reduction in iodine and iodide ion concentrations and enhanced antimicrobial properties observed after 25 months.
In conclusion, both AV biocomponents and Thymol demonstrate an inability to compete with triiodide ions by hydrogen bonding and encapsulation through PVP due to their bigger size.Once iodine is added to AV-PVP-Thymol, AV biocomponents and Thymol are replaced by the smaller triiodide ions.Even after a 25-month period within the formulation, triiodide ions remain shielded by PVP without being released.The majority of those triiodide anions are indeed (according to the UV absorption intensities at 292 nm) smart, symmetrical, linear [I-I-I − ] units, which was also confirmed by Raman data.Analytical findings indicate no alteration in composition, while antimicrobial testing reveals similar inhibition between the 25-month-old sample and the fresh AV-PVP-Thymol-I 2 formulation.Consequently, prolonged storage results in an increased availability of triiodide ions encapsulated by PVP within a sustained-release reservoir [42,43,45,46].The absence of pentaiodide ions in the title biohybrid is not only confirmed by the previously discussed Raman spectrum but also by UV spectral analysis, and also happens within the previously investigated compound AV-PVP-Thyme-I 2 [42].The presence of a broad band around 444 nm in the purple curve for PVP-I 2 indicates the presence of I 5 − units, which is lacking in AV-PVP-Thymol-I 2 (red curve) and AV-PVP-Thymol-I 2 (after 25 months, light blue curve) (Figure 6, Table 2).
Similar results were also found in the previously investigated AV-PVP-Thyme-I 2 [42].However, the results in the title compound are more pronounced because it contains pure Thymol, while the Thyme extract is composed of many constituents.

Fourier-Transform Infrared (FTIR) Spectroscopy
The FTIR spectra of AV-PVP-Thymol and AV-PVP-Thyme-I 2 confirm the purity and similarity of the formulations (Figure 7).
However, the results in the title compound are more pronounced because it contains pure Thymol, while the Thyme extract is composed of many constituents.

Fourier-Transform Infrared (FTIR) Spectroscopy
The FTIR spectra of AV-PVP-Thymol and AV-PVP-Thyme-I2 confirm the purity and similarity of the formulations (Figure 7).The FTIR spectra of AV-PVP-Thymol and AV-PVP-Thymol-I 2 are almost congruent and in agreement with our previous work (Figure 7) [42,43,45,46].AV-PVP-Thymol-I 2 and its 25-month-old formulation show almost the same absorbance pattern and intensity.In AV-PVP-Thymol-I 2 , the bands between 3700 and 3000 cm −1 display an increased absorbance compared to AV-PVP-Thymol and the 25-month-old formulation (Figure 7).The enhanced absorption intensity of the available -COOH and -COH functional groups in AV-PVP-Thymol-I 2 points to a decrease in hydrogen bonding after adding iodine to AV constituents and Thymol.The hydroxyl and carboxyl groups absorb more because they are less encapsulated by hydrogen bonding with the PVP carbonyl groups.Consequently, adding iodine into the formulation means a replacement of those compounds by triiodide ions on the PVP backbone.However, after 25 months of storage, the hydrogen bonding increased again, indicating iodine release and replacement with AV or Thymol biocomponents (Figure 7).The same increase in intensities after adding iodine happened in the region between 1500 and 1000 cm −1 for the in-plane bending and twisting vibrations of the methyl and methylene groups (AV, Thymol), as well as the stretching vibrations of the C-O and C-N groups (PVP, Thymol, AV) (Figure 7, Table 3).
Meanwhile, a mixed picture is portrayed in the region between 3000 and 2800 cm −1 with higher absorption intensities for the title formulation at 2990 and 2855 cm −1 related to symmetric and asymmetric C-H stretching vibrations, respectively (Figure 7, Table 2) [42,43,45,46].These bands show higher absorbance after adding iodine and therefore belong to the released Thymol and the AV components.Iodine competes with the latter for the PVP carbonyl groups, forms triiodide ions, and replaces them.After 25 months, the intensities decrease again.This finding verifies a release of iodine, the formation of carbonyl compounds on the PVP, and, subsequently, the binding of Thymol and AV components by hydrogen bonding (Figure 7, Table 3 (A and C)).The bands around 2949 cm −1 show lower absorption intensities for the iodinated title compound.These bands can be attributed to the C-H stretching vibrations originating from the PVP backbone.The exchange of Thymol and AV components with smart triiodides resulted in an additional red shift for the band at 2953 to 2949 cm −1 , verifying the coiling of the PVP backbone (Table 3) [42,43,[45][46][47].Hydrogen bonding with triiodide molecules leads to higher encapsulation of the C-H bonds in PVP due to the complexation.This is reversed after storing the sample for 25 months, with a clear blue shift back from 2949 to 2953 cm −1 (Figure 7, Table 3).The bands around 1759 to 1665 cm −1 are very weak in both samples and indicate a very low acetylation degree with almost an absence of asymmetric -C=O stretching vibrations derived from PVP-I 2 .It is noteworthy that the absorption frequency changed slightly with a blue shift from 1757 to 1759 cm −1 after adding iodine, while the intensity reduced slightly.These results verify a seamless exchange of hydrogen-bonded Thymol and AV biocomponents with triiodide ions on the carbonyl -C=O of the polymeric PVP backbone.Again, after storing the sample for 25 months, a red shift occurs with higher absorption intensities from 1759 to 1757 cm −1 and a blue shift from 1759 to 1761 cm −1 , reversing the settings and confirming iodine release (Table 3).Another interesting fact is the highest intensity at 1757 and 1661 cm −1 for the 25-month-old sample compared to the other formulations (Figure 7, inlet).This finding confirms an increase in the acetylation degree of asymmetric -C=O stretching vibrations from PVP-I 2 by partial release of triiodide ions.In comparison to our previous investigation with AV-PVP-Thyme-I 2 , storing the sample did not lead to increasing the acetylation degree, nor was there any substantial change in the FTIR analysis.Consequently, Thymol alone is not as effective at retaining the stability of the sustainedrelease reservoir of the formulation as the Thyme extract.The synergistic bio-compounds within the Thyme extract stabilize the formulation even after prolonged storage and do not allow premature release of iodine, hence improving the antimicrobial properties of the AV-PVP-Thyme-I 2 over time [42].As a result, the natural plant extracts from Thyme prevent premature release of iodine by stabilizing the sustained-release reservoir through the synergistic action of biocomponents [42].In comparison, Thymol in AV-PVP-Thymol-I 2 has higher antimicrobial activities but cannot sustain the stability of the formulation over time due to the lack of other supporting, synergistic biocomponents.However, further carbonyl groups related to AV biocomponents like aloin, acemannan, aloe-emodin, emodin, mannose, salicylic acid, uric acid, cinnamic acid, glucomannan, galacturonic acid, and trans-rosmarinic acid seem to engage into hydrogen bonding with each other and/or are reduced to -C-O − or hydroxyl groups (Figure 7, Table 3) [42,43,45,46,62,67,92,93,97].Nevertheless, the higher absorption intensities in the region 3700 and 3000 cm −1 belonging to the AV components and Thymol prove an increase in the number of free carboxylic -COO and -C(=O)OCH 3 ester groups after adding iodine (Figure 7, Table 3) [42,43,45,46,62,67,92,93,97].This assumption is based on the increased absorption intensity of the bands at 3480, 3464, 3425, 3362, 3235, 3169, and 3152 cm −1 after adding iodine and agrees with our previous studies (Figure 7, Table 3) [42,43,45,46,62,67,92,93,97].
In conclusion, adding iodine into AV-PVP-Thymol exchanges all hydrogen-bonded Thymol and AV biocomponents with the smaller triiodide ions with verified increased absorption intensities along with broadening due to their -C=C-, -C=O-, and -COOHgroups in the formulation.As a result of strong hydrogen bonding between the smart triiodide ions and the PVP polymetric matrix, the PVP backbone is coiled and more entangled.This is indicated in the reduced and red-shifted absorptions of the stretching vibrations related to -C-H and methylene groups available in the PVP in the form of [-CH-CH 2 -] n -groups.The polymeric PVP complexes triiodide anions strongly by bending around the stable smart triiodide groups and protects them by forming a sustained-release reservoir [24,42,43,45,46].The coiled or entangled structure is confirmed in the SEM images of the bandages and the face mask in the form of small, circular or cube-like patches covering the surface of the tissues (Figure 3d,b) [42].Storing AV-PVP-Thymol-I 2 for 25 months slightly reverts the previous uptake of iodine by partial exchange with Thymol, which is confirmed by a higher acetylation degree in the older formulation.

X-ray Diffraction (XRD)
AV-PVP-Thymol-I 2 is, according to the XRD analysis, a pure formulation composed of AV, Thymol, PVP, and iodine with a high level of crystallinity (Figure 8).CH2-]ngroups.The polymeric PVP complexes triiodide anions strongly by bending around the stable smart triiodide groups and protects them by forming a sustained-release reservoir [24,42,43,45,46].The coiled or entangled structure is confirmed in the SEM images of the bandages and the face mask in the form of small, circular or cube-like patches covering the surface of the tissues (Figure 3d,b) [42].Storing AV-PVP-Thymol-I2 for 25 months slightly reverts the previous uptake of iodine by partial exchange with Thymol, which is confirmed by a higher acetylation degree in the older formulation.

Previous investigations assign 2Theta values around 12 to 25
• to Thymol [42,83].Our XRD analysis revealed similar 2Theta values at 24.43, 28.83, 30.08, and 30.14 • (Table 4) [45,46,83].After adding iodine, the peak at 28.83 • vanishes and the peak at 30.08 • intensifies in accordance.In comparison with AV-PVP-Thyme-I 2 , the peaks at 2Theta 14.92 and 14.98 • are not available in our title compound with Thymol [42].No further peaks are detected other than the available ones after adding molecular iodine to AV-PVP-Thymol (Figure 8, Table 4).This verifies the purity of the samples as well as the amorphization of iodine, as witnessed in our previous investigations [42,45].

Antimicrobial Activities of AV-PVP-Thymol-I 2
A disc diffusion assay (DD) was used to test the title formulation against 10 reference strains (C.albicans WDCM 00054 Vitroids, Gram-positive bacteria S. pneumonia ATCC 49619, S. aureus ATCC 25923, S. pyogenes ATCC 19615, E. faecalis ATCC 29212, and B. subtilis WDCM0003, as well as the Gram-negative E. coli WDCM 00013 Vitroids, P. mirabilis ATCC 29906, P. aeruginosa WDCM 00026 Vitroids, and K. pneumonia WDCM00097 Vitroids) on sterile discs with concentrations of 11, 5.5, and 2.75 µg/mL.Additionally, polyglycolic acid (PGA) sutures, cotton bandages and KN95 and surgical face masks were coated with AV-PVP-Thymol-I 2 at a concentration of 11 µg/mL against the same 10 reference pathogens.Ethanol and water were used as negative controls and showed no inhibitory action.All the results were compared to the general antibiotics gentamycin (G) and nystatin (NY) (Table 5).

Strain
We tested the control formulation PVP-I 2 to judge the antimicrobial effectivity of our title compound AV-PVP-Thymol-I 2 (Table 6).All 10 pathogens are more susceptible to the title compound with only one resistance against P. mirabilis ATCC 29906 (Table 6).In comparison, two strains, P. mirabilis ATCC 29906 and P. aeruginosa WDCM 00026, are resistant to PVP-I 2 (Table 6).The inhibitory action of the previously reported AV-PVP-I 2 is much lower in comparison to AV-PVP-Thymol-I 2 against the same selection of pathogens [46].Adding AV enhances the antimicrobial properties of the formulation.These findings confirm the superiority of the title compound compared to the control PVP-I 2 and AV-PVP-I 2 (Table 6).As a result, adding Thymol and AV increased the susceptibility of the microorganisms against the title formulation (Table 6).
Another interesting finding is the high inhibitory action of pure Thymol dissolved in ethanol at a concentration of 100 µg/mL (Table 6).In disc diffusion studies, Thymol alone shows high antimicrobial activity against Gram-negative bacteria (ZOI = 39 and 34 mm), while AV-PVP-Thymol-I 2 (ZOI = 17 and 19 mm) shows activity towards K. pneumoniae WDCM 00097 and E. coli WDCM 00013, respectively (Table 6).P. aeruginosa WDCM 00026 is resistant against ethanolic Thymol solutions, but is inhibited by with a ZOI of 15 mm (Table 6).The lower Thymol concentration of the title formulation results in reduced susceptibility of Gram-negative bacteria, while Gram-positive bacteria do not differ much (Table 6).Nevertheless, improved antifungal properties are evident against C. albicans WDCM 00054 for pure Thymol, with a ZOI of 45 mm compared to 60 mm in AV-PVP-Thymol-I 2 (Table 6).The title formulation presents a better tool with lower Thymol concentrations in synergy with AV biocomponents within the polymeric iodophor as a sustained-release reservoir for molecular iodine.Zhou et al. studied pure Thymol at a concentration of 100 µg/mL against S. aureus strains and reported that a slight resistance phenomenon occurs after 30 generation passages with Thymol [78].Thymol showed fatal activity at a concentration of 100 µg/mL against several strains of the multi-drug-resistant pathogen S. aureus by increasing cell membrane permeability through depleting NADPH [78].The authors also indicated that a concentration of 400 µg/mL did not result in resistance against Thymol.They suggested that these results are due to the higher concentration of 400 µg/mL and the multifaceted targets of Thymol within the pathogen [78].A lower concentration of Thymol (100 µg/mL) within the synergetic formulation of a plant-based iodophor AV-PVP-Thymol-I 2 could present a better strategy to combat bacterial resistance.
The formulation was kept for 25 months and again underwent antimicrobial testing.The formulation AV-PVP-Thymol-I 2 achieved a lower ZOI and therefore showcased lower inhibitory action against the same 10 microorganisms after 25 months of storage in comparison to the fresh sample (Table 5).All ZOIs reduced by almost half, although the UV-vis analysis showed higher absorbance in almost all regions compared to the fresh sample (Figure 6b).
Compared to the plant-based AV-PVP-Thyme-I 2 formulation, AV-PVP-Thymol-I 2 shows less inhibitory action after 25 months of storage (Table 6) [42].These results originate from missing synergetic bio-compounds, which enrich the natural plant extract of Thyme, in our previous study [42].The synergistic action of the Thyme-based bio-compounds prevent the premature release of iodine.Therefore, they enhance the encapsulation of triiodide ions in the polymeric PVP complex, prevent decomposition, and mitigate the sustained-release reservoir mechanism of AV-PVP-Thyme-I 2 [24,42].
In conclusion, incorporating pure Thymol into formulations highly enhances the antimicrobial action of the formulation AV-PVP-Thymol-I 2 (Table 5).However, this effect diminishes slowly after storing the formulation for two years due to the lack of other synergetic natural bio-compounds, which assist the complexation of triiodides.During storage, Thymol seems to compete over time with smart triiodides for the hydrogen-bonded positions on the carbonyl oxygen of the PVP.This suggestion is undermined by the strong hydrogen bonding capability of Thymol [79].
Int. J. Mol.Sci.2024, 25, x FOR PEER REVIEW 19 of 34 diminishes slowly after storing the formulation for two years due to the lack of other synergetic natural bio-compounds, which assist the complexation of triiodides.During storage, Thymol seems to compete over time with smart triiodides for the hydrogen-bonded positions on the carbonyl oxygen of the PVP.This suggestion is undermined by the strong hydrogen bonding capability of Thymol [79].
The disc diffusion (DD) tests were performed with concentrations of 11, 5.5, 2.75, and 1.38 µg/mL (Figure 9, Table 5).AV-PVP-Thymol-I2 is a strong antifungal agent on discs at concentrations of 11, 5.5, and 2.75 µg/mL against C. albicans WDCM 00054, even after storing for 25 months and with methanol (72 mm) (Figure 9, Tables 5 and 6).Among the Gram-positive pathogens, S. aureus ATCC 25932 is the most susceptible to the title compound with 35, 30, and 25 mm ZOIs (Figure 9b, Tables 5 and 6).The Gram-negative microorganism E. coli WDCM 00013 is the most inhibited amongst its peers with 19, 16, and 13 mm ZOIs (Figure 9c, Tables 5  and 6).In comparison, similar patterns of susceptibility exist in the previously studied formulation AV-PVP-Thyme-I2 (Table 6) [42].Both formulations, AV-PVP-Thymol-I2 and AV-PVP-Thyme-I2, are strong antifungals on discs, followed by Gram-positive and finally Gram-negative reference strains (Table 6).This pattern of inhibitory action is due to the structural outer-cell-membrane morphology of the related pathogens.C. albicans and Gram-positive pathogens are more susceptible to our formulations due to their less negatively charged, lipophilic outer membranes.Gram-negative pathogens have more negatively charged outer cell membranes and are more hydrophilic.Their porin channels allow passage to only negatively charged, small hydrophilic ions like iodide and triiodide ions.Iodine molecules are lipophilic and pass easily through the lipophilic peptidoglycan layers present in Gram-positive pathogens.Another discouraging factor is the motility of Gram-negative microorganisms.Indeed, the higher the motility (from top to bottom in Tables 5 and 6), the less susceptible the bacteria are towards our formulations.Motility is inversely related to the susceptibility towards our polymeric iodophors.Consequently, both formulations face resistance by the swarming bacteria P. mirabilis ATCC 29906 (Tables 5 and 6).
As a result, discs impregnated with AV-PVP-Thymol-I2 show strong antifungal activities against C. albicans WDCM 00054, followed by the Gram-positive bacteria S. aureus ATCC 25923, B. subtilis WDCM 00003, and S. pyogenes ATCC 19615.Good results were recorded towards E. faecalis ATCC 29212 and S. pneumoniae ATCC 49619 as well.Intermediate susceptibility towards the Gram-negative bacteria K. pneumoniae WDCM 00097 (ZOI = 17), E. coli WDCM 00013 (ZOI = 19), and P. aeruginosa WDCM 00026 (ZOI = 15) compared to the antibiotic Gentamycin is promising (Table 5).All the results in Table 5 verify the AV-PVP-Thymol-I 2 is a strong antifungal agent on discs at concentrations of 11, 5.5, and 2.75 µg/mL against C. albicans WDCM 00054, even after storing for 25 months and with methanol (72 mm) (Figure 9, Tables 5 and 6).Among the Gram-positive pathogens, S. aureus ATCC 25932 is the most susceptible to the title compound with 35, 30, and 25 mm ZOIs (Figure 9b, Tables 5 and 6).The Gram-negative microorganism E. coli WDCM 00013 is the most inhibited amongst its peers with 19, 16, and 13 mm ZOIs (Figure 9c, Tables 5 and 6).In comparison, similar patterns of susceptibility exist in the previously studied formulation AV-PVP-Thyme-I 2 (Table 6) [42].Both formulations, AV-PVP-Thymol-I 2 and AV-PVP-Thyme-I 2 , are strong antifungals on discs, followed by Gram-positive and finally Gramnegative reference strains (Table 6).This pattern of inhibitory action is due to the structural outer-cell-membrane morphology of the related pathogens.C. albicans and Gram-positive pathogens are more susceptible to our formulations due to their less negatively charged, lipophilic outer membranes.Gram-negative pathogens have more negatively charged outer cell membranes and are more hydrophilic.Their porin channels allow passage to only negatively charged, small hydrophilic ions like iodide and triiodide ions.Iodine molecules are lipophilic and pass easily through the lipophilic peptidoglycan layers present in Gram-positive pathogens.Another discouraging factor is the motility of Gram-negative microorganisms.Indeed, the higher the motility (from top to bottom in Tables 5 and 6), the less susceptible the bacteria are towards our formulations.Motility is inversely related to the susceptibility towards our polymeric iodophors.Consequently, both formulations face resistance by the swarming bacteria P. mirabilis ATCC 29906 (Tables 5 and 6).
As a result, discs impregnated with AV-PVP-Thymol-I 2 show strong antifungal activities against C. albicans WDCM 00054, followed by the Gram-positive bacteria S. aureus ATCC 25923, B. subtilis WDCM 00003, and S. pyogenes ATCC 19615.Good results were recorded towards E. faecalis ATCC 29212 and S. pneumoniae ATCC 49619 as well.Intermediate susceptibility towards the Gram-negative bacteria K. pneumoniae WDCM 00097 (ZOI = 17), E. coli WDCM 00013 (ZOI = 19), and P. aeruginosa WDCM 00026 (ZOI = 15) compared to the antibiotic Gentamycin is promising (Table 5).All the results in Table 5 verify the potential use of AV-PVP-Thymol-I 2 as a surface contact-killing agent and antibacterial coating material.
In general, the inhibitory zones for the title formulation AV-PVP-Thymol-I2 on sutures are much higher than compared to the Thyme formulation.AV-PVP-Thymol-I2 exhibited a ZOI of 9 mm for the Gram-positive bacteria S. aureus ATCC 25923, followed by ZOI = 6 for B. subtilis WDCM 00003 and E. faecalis ATCC 29212 (Table 5, Figure 10b).Further inhibited Gram-positive bacteria include S. pyogenes ATCC 19615 and S. pneumoniae ATCC 49619, both with ZOI = 2 (Table 5).K. pneumoniae WDCM 00097 and E. coli WDCM 00013 are the only susceptible Gram-negative pathogens on dip-coated surgical sutures with a ZOI of 3 mm, while the rest are all resistant (Table 5, Figure 10).
In summary, our research suggests that absorbable, surgical PGA sutures treated with our unique formulation AV-PVP-Thymol-I2 exhibit promise as antimicrobial agents in the prevention of surgical-site infections caused by C. albicans WDCM 00054, Grampositive bacteria E. faecalis ATCC 29212, S. aureus ATCC 25923, and B. subtilis WDCM 00003, as well as Gram-negative bacteria K. pneumoniae WDCM 00097 and E. coli WDCM 00013.
In general, the inhibitory zones for the title formulation AV-PVP-Thymol-I 2 on sutures are much higher than compared to the Thyme formulation.AV-PVP-Thymol-I 2 exhibited a ZOI of 9 mm for the Gram-positive bacteria S. aureus ATCC 25923, followed by ZOI = 6 for B. subtilis WDCM 00003 and E. faecalis ATCC 29212 (Table 5, Figure 10b).Further inhibited Gram-positive bacteria include S. pyogenes ATCC 19615 and S. pneumoniae ATCC 49619, both with ZOI = 2 (Table 5).K. pneumoniae WDCM 00097 and E. coli WDCM 00013 are the only susceptible Gram-negative pathogens on dip-coated surgical sutures with a ZOI of 3 mm, while the rest are all resistant (Table 5, Figure 10).
In summary, our research suggests that absorbable, surgical PGA sutures treated with our unique formulation AV-PVP-Thymol-I 2 exhibit promise as antimicrobial agents in the prevention of surgical-site infections caused by C. albicans WDCM 00054, Gram-positive bacteria E. faecalis ATCC 29212, S. aureus ATCC 25923, and B. subtilis WDCM 00003, as well as Gram-negative bacteria K. pneumoniae WDCM 00097 and E. coli WDCM 00013.
We applied the title formulation AV-PVP-Thymol-I 2 to both surgical face masks (M) and KN95 masks, investigating its inhibitory effects (Table 5).Our findings verify the previously observed patterns across different pathogens.The highest inhibitory action was demonstrated against C. albicans WDCM 00054, once again verifying the very strong antifungal action with ZOI = 66, 84, and 80 mm for blue (M B ) and white (M W ) mask layers and KN95 masks, respectively (Table 5, Figure 11).and safety of face masks were questioned, and they added to environmental pollution in the form of huge waste piles all over the world [8,42,43,[118][119][120][121][122][123][124].Employing methods such as reusing face masks by treating them with antimicrobial surface agents can help mitigate microbial proliferation.Subsequently, such measures would reduce environmental pollution, enable the safety of low-income populations, and promote sustainability.
Similar but more modest results were observed for the entire range of pathogens for the Thyme formulation AV-PVP-Thyme-I 2 [42].Moreover, the inhibitory effects against C. albicans WDCM 00054 are notably improved when AV-PVP-Thyme-I 2 is incorporated into KN95 masks (80 mm), followed by the white surgical face mask layer (55 mm) and the blue layer (45 mm) [42].
In conclusion, AV-PVP-Thymol-I 2 demonstrates potent inhibitory effects against various microorganisms on both surgical face masks and KN95 face masks.These include the fungal strain C. albicans WDCM 00054 and Gram-positive bacteria such as S. aureus ATCC 25923 and B. subtilis WDCM 00003 (Table 5).Additionally, Gram-negative pathogens like K. pneumoniae WDCM 00097, E. coli WDCM 00013, and P. aeruginosa WDCM 00026 exhibit heightened susceptibility when tested on surgical face masks and KN95 masks in comparison to the impregnated discs.These results are in accordance with the previously studied formulation AV-PVP-Thyme-I 2 [42].Nevertheless, the latter exhibits lessened inhibitory action against some pathogens.However, P. mirabilis ATCC 29906 is exclusively inhibited when AV-PVP-Thyme-I 2 is impregnated onto KN95 masks, while it is resistant completely to AV-PVP-Thymol-I 2 [42].
In conclusion, AV-PVP-Thymol-I2 demonstrates potent inhibitory effects against various microorganisms on both surgical face masks and KN95 face masks.These include the fungal strain C. albicans WDCM 00054 and Gram-positive bacteria such as S. aureus ATCC 25923 and B. subtilis WDCM 00003 (Table 5).Additionally, Gram-negative pathogens like K. pneumoniae WDCM 00097, E. coli WDCM 00013, and P. aeruginosa WDCM 00026 exhibit heightened susceptibility when tested on surgical face masks and KN95 masks in comparison to the impregnated discs.These results are in accordance with the previously studied formulation AV-PVP-Thyme-I2 [42].Nevertheless, the latter exhibits lessened inhibitory action against some pathogens.However, P. mirabilis ATCC 29906 is exclusively inhibited when AV-PVP-Thyme-I2 is impregnated onto KN95 masks, while it is resistant completely to AV-PVP-Thymol-I2 [42].
As a result, AV-PVP-Thymol-I2-impregnated cotton bandages, sutures, face masks, and KN95 masks have the potential to be used in mitigating microbial infections.In general, the inhibitory action is highest against C. albicans WDCM 00054, followed by Grampositive and Gram-negative pathogens.
The overall susceptibility pattern remains consistent across all impregnated materials such as discs, sutures, cotton bandages, surgical face masks, and KN95 masks.Accordingly, the highest to lowest susceptibility, starting with C. albicans WDCM 00054, followed by Gram-positive and, finally, Gram-negative microorganisms, is presented in Table 5 in descending order.Bacterial morphology, size, and motility determine the susceptibility towards AV-PVP-Thymol-I2.Nevertheless, depending on the impregnated material (disc, suture, cotton bandage, KN95 mask, and surgical face mask), significant inhibition zones are observed.Bio-compounds from AV and Thymol penetrate the cell membranes and initiate cell death.However, the key factor remains the release of triiodide ions from the Gram-positive and Gram-negative pathogens exhibit inhibition zones around 23, 22, and 21 mm (Table 5).In comparison, similar patterns were observed in our previous study on AV-PVP-Thyme-I 2 , except C. albicans WDCM 00054 demonstrated the largest inhibition zone (53 mm) [42].
As a result, AV-PVP-Thymol-I 2 -impregnated cotton bandages, sutures, face masks, and KN95 masks have the potential to be used in mitigating microbial infections.In general, the inhibitory action is highest against C. albicans WDCM 00054, followed by Gram-positive and Gram-negative pathogens.
The overall susceptibility pattern remains consistent across all impregnated materials such as discs, sutures, cotton bandages, surgical face masks, and KN95 masks.Accordingly, the highest to lowest susceptibility, starting with C. albicans WDCM 00054, followed by Gram-positive and, finally, Gram-negative microorganisms, is presented in Table 5 in descending order.Bacterial morphology, size, and motility determine the susceptibility towards AV-PVP-Thymol-I 2 .Nevertheless, depending on the impregnated material (disc, suture, cotton bandage, KN95 mask, and surgical face mask), significant inhibition zones are observed.Bio-compounds from AV and Thymol penetrate the cell membranes and initiate cell death.However, the key factor remains the release of triiodide ions from the sustained-release reservoir PVP.These moieties decompose to free molecular iodine, which is a small, lipid-soluble molecule, able to diffuse through the lipophilic peptidoglycan layers of Gram-positive pathogens, facilitating cell death.Gram-negative pathogens are less affected by molecular iodine, Thymol, and AV biocomponents.Porin channels in Gram-negative bacteria allow the diffusion of hydrophilic triiodide ions, iodide ions, and small phenolic acids through the outer membranes.
which is a small, lipid-soluble molecule, able to diffuse through the lipophilic peptidoglycan layers of Gram-positive pathogens, facilitating cell death.Gram-negative pathogens are less affected by molecular iodine, Thymol, and AV biocomponents.Porin channels in Gram-negative bacteria allow the diffusion of hydrophilic triiodide ions, iodide ions, and small phenolic acids through the outer membranes.
Storing the stock solution AV-PVP-Thymol-I2 for 25 months results in slightly reduced antimicrobial activities.In contrast, the Thyme-extract-based AV-PVP-Thyme-I2 In contrast, the inhibition of Gram-negative bacteria correlates with their motility.The most active, swarming bacteria, P. mirabilis ATCC 29906, is only inhibited on KN95 by AV-PVP-Thyme-I 2 [42].Nevertheless, even the motile Gram-negative bacteria were inhibited very well by the uniformly coated materials featuring small, spherical, cubelike patches of AV-PVP-Thymol-I 2 on bandages, face masks, KN95, and sutures.The title bioformulation AV-PVP-Thymol-I 2 has the same pattern of antimicrobial activity as our previous biohybrid AV-PVP-Thyme-I 2 (Table 5) [42].Both formulations contain Thymol as a major ingredient and therefore depict similar results.AV-PVP-Thymol-I 2 is a strong antifungal on all tested materials except cotton bandages in comparison to AV-PVP-Thyme-I 2 [42].AV-PVP-Thymol-I 2 showcases an inhibitory zone (ZOI) of 30 mm, while AV-PVP-Thyme-I 2 has a ZOI of 53 mm [42].The disc diffusion studies on sterile discs and PGA sutures (S) have generally higher ZOIs in the title formulation, AV-PVP-Thymol-I 2 , compared to AV-PVP-Thyme-I 2 [42].The results on surgical masks (M W /M B ) show stronger inhibition of the reference strains by AV-PVP-Thymol-I 2 [42].Impregnated KN95 and bandages (B) achieve better inhibition in the Thyme formulation [42].
Storing the stock solution AV-PVP-Thymol-I 2 for 25 months results in slightly reduced antimicrobial activities.In contrast, the Thyme-extract-based AV-PVP-Thyme-I 2 shows enhanced antimicrobial properties against the same 10 pathogens after 18 months [42].Consequently, Thyme extract biocomponents in AV-PVP-Thyme-I 2 , like Thymol, carvacrol and rosmarinic acid, are responsible (together with AV components) for sustaining the inhibitory action during its storage [42].The title formulation AV-PVP-Thymol-I 2 consists of Thymol alone in combination with AV biocomponents.During its storage for up to 25 months, the polymeric iodophor PVP-I 2 slowly releases iodine due to the lack of key ingredients carvacrol, rosmarinic acid, and others originating from the natural Thyme extract [42].Thymol alone cannot sustain the sustained-release reservoir.Additionally, Thymol has strong hydrogen bonding properties and, over time, replaces triiodide ions on the PVP backbone, thus slowly reducing the inhibitory action of AV-PVP-Thymol-I 2 (Figure 14) [79].
vacrol and rosmarinic acid, are responsible (together with AV components) for sustaining the inhibitory action during its storage [42].The title formulation AV-PVP-Thymol-I2 consists of Thymol alone in combination with AV biocomponents.During its storage for up to 25 months, the polymeric iodophor PVP-I2 slowly releases iodine due to the lack of key ingredients carvacrol, rosmarinic acid, and others originating from the natural Thyme extract [42].Thymol alone cannot sustain the sustained-release reservoir.Additionally, Thymol has strong hydrogen bonding properties and, over time, replaces triiodide ions on the PVP backbone, thus slowly reducing the inhibitory action of AV-PVP-Thymol-I2 (Figure 14) [79].As a conclusion, the obtained data from the antimicrobial testing of AV-PVP-Thymol-I2 on discs, surgical sutures, bandages, face masks, and KN95 masks are promising, showing its successful use as an antimicrobial agent.AV-PVP-Thymol-I2 has the potential to mitigate and reduce inflammation as well as wound and surgical-site infections.It can be promising as a surface-killing agent and in reducing the airborne transmission of microbes.Further investigations are planned to verify its potential uses through in vivo and toxicity studies.

Materials
In December, we collected AV leaves from the botanical garden situated at the Ajman University campus in Ajman, UAE.Reference strains including E. coli WDCM 00013  As a conclusion, the obtained data from the antimicrobial testing of AV-PVP-Thymol-I 2 on discs, surgical sutures, bandages, face masks, and KN95 masks are promising, showing its successful use as an antimicrobial agent.AV-PVP-Thymol-I 2 has the potential to mitigate and reduce inflammation as well as wound and surgical-site infections.It can be promising as a surface-killing agent and in reducing the airborne transmission of microbes.Further investigations are planned to verify its potential uses through in vivo and toxicity studies.

Preparation of Aloe vera (AV) Extract
The Aloe vera (A.barbadensis Miller) leaves were harvested in December from the botanical garden of Ajman University during the early morning hours [42,43,45,46].Within a span of 10 min, leaves measuring 35 to 50 cm were carefully washed with water and then rinsed thoroughly with distilled water, then absolute ethanol, and subsequently rinsed multiple times with ultrapure water.They were then allowed to air dry for 1 h.Once dried, the AV leaves were cut with a sterile knife to facilitate the collection of the mucilaginous gel into a sterile beaker.The gel was then transferred to a sterile mixer and homogenized for 10 min until a consistent texture was achieved.Afterwards, the AV gel was centrifuged for 40 min at 4000 rpm (3K 30; Sigma Laborzentrifugen GmbH, Osterode am Harz, Germany).The resulting supernatant, possessing a light-yellow hue, was promptly transferred into a sterile brown screw-capped bottle and placed in a refrigerator at 3 • C until further use.

Preparation of AV-PVP-Thymol-I 2
AV-PVP-Thymol-I 2 was synthesized through a straightforward one-pot process.Firstly, 2 mL of pure AV gel was dispensed into a sterile beaker.Subsequently, 2 mL of a recently prepared solution containing 1 g of polyvinylpyrrolidone K-30 (PVP) dissolved in 10 mL of distilled water was introduced into the beaker under stirring at ambient temperature.Following this, 2 mL of Thymol solution (0.15 g in 10 mL of ethanol, 100 µg/mL) was slowly added to the mixture while stirring.Finally, 2 mL of a freshly prepared iodine solution (0.05 g of iodine in 3 mL of absolute ethanol) was incorporated into the mixture under continuous stirring and at room temperature.The resulting formulation, AV-PVP-Thymol-I 2 , was promptly transferred into a screw-capped sterile glass sample tube and stored in darkness at 3 • C in a refrigerator for subsequent use.

Scanning Electron Microscopy (SEM) and Energy-Dispersive X-ray Spectroscopy (EDX)
The VEGA3 from TESCAN (Brno, Czech Republic) was utilized for scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) examination, operating at 15 kV.A solution of the AV-PVP-Thymol-I 2 formulation was diluted with distilled water, then applied onto a carbon-coated copper grid and subsequently dried.Following this, a gold coating was applied using the Quorum Technology Mini Sputter Coater (Brno, Czech Republic).SEM analysis provided insights into the morphology, while EDS analysis verified the purity of the formulation AV-PVP-Thymol-I 2 .

UV-Vis Spectrophotometry (UV-Vis)
The analysis of AV-PVP-Thymol-I 2 was conducted using a UV-Vis spectrophotometer, specifically the model 2600i manufactured by Shimadzu in Kyoto, Japan.Measurements were taken across the wavelength spectrum from 195 to 800 nm.

Raman Spectroscopy
The composition AV-PVP-Thymol-I 2 underwent analysis under ambient conditions using a RENISHAW system located in Gloucestershire, UK, featuring an optical microscope.The sample was introduced into a cuvette measuring 1 cm × 1 cm and positioned in front of the laser beam, which had an excitation wavelength of 785 nm.Utilizing a 50× magnification confocal microscope, the beam was focused to a spot diameter of 2 microns onto the sample.Scattered light was captured by a CCD-based monochromator with a spectral range spanning 50-3400 cm −1 and a spectral resolution of −1 cm −1 .The monochromator had an output power of 0.5% and an integration time of approximately 30 s.
to the nearest millimetre.The absence of a discernible inhibition zone indicated a lack of inhibition against the reference microbial strain.

Statistical Analysis
SPSS software (version 17.0, SPSS Inc., Chicago, IL, USA) was employed for statistical analysis, with data presented in mean values.The significance between groups was determined through one-way ANOVA.Statistical significance was defined as p < 0.05.

Conclusions
Antimicrobial resistance poses an ongoing, dangerous challenge to humankind.Microorganisms adapt various mechanisms to escape efforts aimed at controlling their spread.Multi-drug-resistant ESKAPE pathogens are already escalating rates of illness and death worldwide.Novel, cost-effective, sustainable, and easily implementable strategies are pivotal to address this issue.
A promising avenue includes investigating natural antimicrobial formulations with synergetic bio-compounds within natural plant extracts.Plants develop comprehensive defence mechanisms through the synergistic action of bio-compounds.We combined AV gel and Thymol within a sustained-release reservoir of PVP-I 2 .The microbicide iodine, complexed into PVP, enhances the antimicrobial properties of the plant-derived materials from AV and Thymol.The resulting formulation, AV-PVP-Thymol-I 2 , has demonstrated potent antifungal activity against C. albicans WDCM 00054 and the selection of Grampositive, as well as Gram-negative, microorganisms when uniformly applied to sterile discs, bandages, sutures, and surgical and KN95 face masks.The SEM of the coated material shows a homogenous surface with small patches, which results in high inhibitory action against the selected pathogens.
The title formulation still exerts inhibitory action after a storage period of 25 months, but at lower rates.However, in comparison to AV-PVP-Thymol-I 2 , the title compound AV-PVP-Thymol-I 2 exhibited two unexpected properties.Firstly, the incorporation of Thymol instead of Thyme extract enhanced the antimicrobial properties of the title formulation AV-PVP-Thymol-I 2 strongly against the selection of microorganisms.Secondly, storage for more than two years (25 months) reduces the susceptibility of the reference strains against AV-PVP-Thymol-I 2 slowly, while the opposite happened to AV-PVP-Thyme-I 2 .Using Thymol instead of a Thyme extract has the advantage of much higher inhibitory action, but this property slowly decreases over a storage time of 2 years.Consequently, Thyme-extract-mediated antimicrobial activity is a result of synergetic bio-compounds and therefore stabilizes the sustained-release reservoir.Meanwhile, Thymol in AV-PVP-Thymol-I 2 slowly competes over a span of 2 years with the triiodide ions, resulting in their premature release.
The incorporation of AV-PVP-Thymol-I 2 into face masks exhibited significant inhibition against nine reference strains except for P. mirabilis ATCC 29906.Nevertheless, the
UV-vis absorption signals with concentration of 0.11 µg/mL.** The broad bands overlap and several peaks related to AV compounds, iodine moieties, and thymol/carvacrol cannot be observed.vw = very weak, br = broad, s = strong, vs = very strong, m = intermediate, sh = shoulder.