Synthesis of Enantiopure (S)-Atenolol by Utilization of Lipase-Catalyzed Kinetic Resolution of a Key Intermediate

(S)-Atenolol ((S)-2-(4-(2-Hydroxy-3-(isopropylamino)propoxy)phenyl)acetamide) has been synthesized in >99% enantiomeric excess (ee) with the use of Candida antarctica lipase B from Syncozymes (Shanghai, China), in a kinetic resolution of the corresponding racemic chlorohydrin. A catalytic amount of base was used in deprotonation of the phenol building block. The enantiopurity of the chlorohydrin building block remained unchanged upon subsequent amination to yield the final drug. All four steps in the synthesis protocol have been optimized compared to previously reported methods, which makes this new protocol more sustainable and in accordance with green chemistry principles. The overall yield of (S)-atenolol was 9.9%, which will be further optimized.


Introduction
The American Heart Association reported in March 2019 their update on heart diseases and stroke statistics.The report states that high blood pressure concerned 46% of the total population at ages 20 years and older in the United States between 2013-2016.It was the cause of death for 82,735 Americans in 2016 and cost the American society approximately USD 55.9 billion in the period 2014-2015 [1].In 2018, cardiovascular treatment made up 4.9% of the total pharmaceutical market in Norway, which corresponded to NOK 1.17 million [2].A class of drugs that have been used in the treatment of both cardiovascular and non-cardiovascular diseases are the β-adrenergic blocking agents (beta-blockers).Approximately 300,000 patients in Norway use beta-blockers [3].Worldwide, the use of beta-blockers increases year by year and the sales are estimated to account for USD 13,684 million by 2030 [4].
The highly polar cardio selective β1-antagonist atenolol is selective towards β1receptors found in the heart.This drug is used in the treatment of hypertension, angina pectoris and arrythmia.Atenolol is one of the most widely used beta-blockers clinically and is often used as a reference drug for comparisons with other antihypertensives.The drug might, however, be even more effective in preventing myocardial infarction [5].
Atenolol is manufactured with enantiomerically pure active pharmaceutical ingredient (API) as Atpure ® by Emcure Pharmaceuticals (Pune, India), and with racemic API under the names Tenormin ® , Mylan ® and others.The eudismic ratio is 46 in favour of (S)-to (R)-atenolol [6], and studies in rats show that the R-enantiomer has no effect.While the racemic drug causes a lowering of the heart rate, this side effect is not observed with enantiopure (S)-atenolol [7].
Several synthesis protocols for producing enantiopure (S)-atenolol have been published.Emcure Pharmaceuticals uses enantiopure epichlorohydrin to produce enantiopure (S)-atenolol from deprotonated 2-(4-hydroxyphenyl)acetamide.The specific rotation of the final drug (S)-atenolol of [α] 25 D = −17.1 (1.0, 1N HCl) is reported [8].Dwivedee et al. also started with deprotonation of 2-(4-hydroxyphenyl)acetamide gaining only the epoxide 2-(4-(2-oxiran-2-ylmethoxy)phenyl)benzeneacetamide.Reaction of this epoxide with acetyl chloride in methanol gave the racemic 4-(3-chloro-2 hydroxypropoxy)benzeneacetamide, which was resolved by several lipase preparations and vinyl acetate as the acyl donor.The authors claim to have formed (S)-atenolol from (S)-4-(3-chloro-2-hydroxypropoxy)benzeneacetamide [9].This is not possible according to their reaction conditions, since addition of isopropylamine in water to (S)-4-(3-chloro-2-hydroxypropoxy)benzeneacetamide leads to (R)-atenolol.The authors do not report any specific rotation of their (claimed) (S)-atenolol product, nor of their enantiopure building blocks.Agustian et al. present a similar study of (S)-atenolol synthesis using several lipase preparations to resolve the racemic 4-(3-chloro-2 hydroxypropoxy)benzeneacetamide [10].The authors present no evidence of the absolute configuration of the product, nor any yields or evidence of the enantiomeric excess (ee) of the product.Sikora et al. reported in 2020 the kinetic resolution of racemic atenolol catalyzed by lipase from Candida rugosa with isopropenyl acetate as the acyl donor, resulting in the acetate of (S)-atenolol in 94% ee.The authors present no evidence of the absolute configuration of this acetate, nor of the unreacted (R)-atenolol [11,12].The authors have previously published several articles on lipase catalyzed kinetic resolution of racemic atenolol with the amide of (S)-atenolol presented as the acetate [13][14][15].(S)-Atenolol has been synthesized in 98% ee in a seven-step method using Jacobsen's catalyst ((R,R)-salen Co(III)OAc) [16], and in 94% ee through kinetic resolution of racemic atenolol using lipase from Pseudomonas cepacia [17].We have produced the enantiopure building block (R)-4-(3-chloro-2 hydroxypropoxy)benzeneacetamide starting with a deprotonation of 2-(4-hydroxyphenyl)acetamide with sodium hydroxide, and by using lipase B from Candida antarctica (CALB) in the kinetic resolution of the racemic chlorohydrin 4-(3-chloro-2 hydroxypropoxy)benzeneacetamide, the enantiopure chlorohydrin was obtained with 99% ee [18].We have now improved the yield of the building block and reduced the amounts of reactants used.The enantiopure drug (S)-atenolol has been synthesized from the enantiopure chlorohydrin.
When stoichiometric amounts of sodium hydroxide were used to deprotonate the phenolic proton of 1, a small peak at t R = 13.37 min was seen in the HPLC chromatogram together with the desired product chlorohydrin 2a (t R = 12.06 min) and the epoxide 2b (t R = 9.58 min), see Figure 1.LC-MS analysis of the reaction mixture on an AQUITY UPLC BEH C18-column with an isocratic mobile phase composition of water and acetonitrile and (30:70) with 1% formic acid and a flow of 0.2 mL/min showed a peak with the molecular mass of 382.04 g/mol, which corresponds to the mass of C 19 H 21 N 2 O 5 Na (Figure 2).The compound then has a molecular mass of 358.39 g/mol, which corresponds to compound 2c.We have previously predicted the mechanism of the formation of this dimeric ether compound in syntheses of similar beta-blockers.Here, we also present full characterization of the corresponding by-product in the synthesis of enantiopure (S)-esmolol [19].
When stoichiometric amounts of sodium hydroxide were used to deprotonate the phenolic proton of 1, a small peak at tR = 13.37 min was seen in the HPLC chromatogram together with the desired product chlorohydrin 2a (tR = 12.06 min) and the epoxide 2b (tR = 9.58 min), see Figure 1.LC-MS analysis of the reaction mixture on an AQUITY UPLC BEH C18-column with an isocratic mobile phase composition of water and acetonitrile and (30:70) with 1% formic acid and a flow of 0.2 mL/min showed a peak with the molecular mass of 382.04 g/mol, which corresponds to the mass of C19H21N2O5Na (Figure 2).The compound then has a molecular mass of 358.39 g/mol, which corresponds to compound 2c.We have previously predicted the mechanism of the formation of this dimeric ether compound in syntheses of similar beta-blockers.Here, we also present full characterization of the corresponding by-product in the synthesis of enantiopure (S)-esmolol [19].When two equivalents of epichlorohydrin were added to a solution containing phenol 1 and catalytical amounts of sodium hydroxide, the by-product 2c was not observed after 4 hours of reaction.A proposed mechanism for the reaction of 1 to 2a and 2b with the regeneration of the base is shown in Scheme 2. Phenol 1 is deprotonated to form phenoxide 1′ which can attack epichlorohydrin at carbon 1 or 3 (reaction mechanism a and b, respectively) resulting in phenoxide 2a′ and the epoxide 2b.Phenoxide 2a′ may react in the mixture produced pure 2a, which was resolved in acetonitrile with vinyl butanoate and with lipase B from Candida antarctica (CALB).Addition of isopropylamine in water to (R)-2a (>99% ee) resulted in (S)-atenolol ((S)-4) in >99% ee, 60% yield and 99% purity.
When stoichiometric amounts of sodium hydroxide were used to deprotonate the phenolic proton of 1, a small peak at tR = 13.37 min was seen in the HPLC chromatogram together with the desired product chlorohydrin 2a (tR = 12.06 min) and the epoxide 2b (tR = 9.58 min), see Figure 1.LC-MS analysis of the reaction mixture on an AQUITY UPLC BEH C18-column with an isocratic mobile phase composition of water and acetonitrile and (30:70) with 1% formic acid and a flow of 0.2 mL/min showed a peak with the molecular mass of 382.04 g/mol, which corresponds to the mass of C19H21N2O5Na (Figure 2).The compound then has a molecular mass of 358.39 g/mol, which corresponds to compound 2c.We have previously predicted the mechanism of the formation of this dimeric ether compound in syntheses of similar beta-blockers.Here, we also present full characterization of the corresponding by-product in the synthesis of enantiopure (S)-esmolol [19].When two equivalents of epichlorohydrin were added to a solution containing phenol 1 and catalytical amounts of sodium hydroxide, the by-product 2c was not observed after 4 h of reaction.A proposed mechanism for the reaction of 1 to 2a and 2b with the regeneration of the base is shown in Scheme 2. Phenol 1 is deprotonated to form phenoxide 1 ′ which can attack epichlorohydrin at carbon 1 or 3 (reaction mechanism a and b, respectively) resulting in phenoxide 2a ′ and the epoxide 2b.Phenoxide 2a ′ may react in two ways: by deprotonating a water molecule forming chlorohydrin 2a, thus regenerating the base (reaction mechanism c), or by an intramolecular S N 2-like reaction forming epoxide 2b (reaction mechanism d).HPLC analyses of the product mixtures from the reaction of 1 to 2a and 2b showed that the use of catalytic amounts of base favoured the formation of 2a over the epoxide 2b.When one or two equivalents of base were used relative to 1, 2b and 2c were predominant after 6 h of reaction time.The use of catalytic amounts of base is possible due to the regeneration of the base during the deprotonation of water during the formation of 2a from 2a′.For a similar reason, it is also possible to use less acetic acid than previously reported in the syntheses of other beta-blockers.We have previously used between five and ten equivalents of acetic acid and two to five equivalents of lithium chloride [20].By addition of excess lithium chloride relative to 2b in the reaction mixture and approximately five equivalents of acetic acid relative to 2b, a 52% yield of 2a was achieved.Since the molar masses of 2a and 2b are different and the HPLC analyses yield only relative amounts of each compound in the reaction mixture, the amounts of the reactants are estimated.

Lipase Catalyzed Kinetic Resolution of Chlorohydrin 2a
A CALB catalyzed kinetic resolution of 2a (separation of the enantiomers of 2a on the Chiralcel column is shown in Figure S1 in Supplementary Material) with vinyl butanoate as the acyl donor in acetonitrile produced (R)-2a in 99% ee with 32% yield (Scheme 1, Figure S2).Figures S3 and 3 show the chromatograms of the reaction after 1, 5 and 24 hours of a total of 27 hours reaction time.After 27 hours, no (S)-2a was seen in the chromatogram and the ee of (R)-2a was >99.0% The column used did not allow sufficient separation of the two enantiomers of the butanoic ester (S/R)-3 to measure the enantiopurity of the ester.In order to verify the enantiomer formed, the ester was hydrolyzed and identified as the (S)-3 enantiomer.We The use of catalytic amounts of base is possible due to the regeneration of the base during the deprotonation of water during the formation of 2a from 2a ′ .For a similar reason, it is also possible to use less acetic acid than previously reported in the syntheses of other beta-blockers.We have previously used between five and ten equivalents of acetic acid and two to five equivalents of lithium chloride [20].By addition of excess lithium chloride relative to 2b in the reaction mixture and approximately five equivalents of acetic acid relative to 2b, a 52% yield of 2a was achieved.Since the molar masses of 2a and 2b are different and the HPLC analyses yield only relative amounts of each compound in the reaction mixture, the amounts of the reactants are estimated.

Lipase Catalyzed Kinetic Resolution of Chlorohydrin 2a
A CALB catalyzed kinetic resolution of 2a (separation of the enantiomers of 2a on the Chiralcel column is shown in Figure S1 in Supplementary Material) with vinyl butanoate as the acyl donor in acetonitrile produced (R)-2a in 99% ee with 32% yield (Scheme 1, Figure S2). Figure S3 and Figure 3 show the chromatograms of the reaction after 1, 5 and 24 h of a total of 27 h reaction time.After 27 h, no (S)-2a was seen in the chromatogram and the ee of (R)-2a was >99.0%

Synthesis of Racemic Atenolol (4) and Attempt to Resolve 4 with CALA
Another attempt to synthesizing enantiopure (S)-atenolol was performed in a kinetic resolution of racemic atenolol (4) by use of lipase A from Candida antarctica.Racemic 4 was produced directly from the reaction mixture of 2a and 2b (without the ring opening of 2b with lithium chloride) with addition of isopropylamine in methanol resulting in 4 in 42% yield (Scheme 3).Scheme 3. Atenolol (4) was prepared directly from the reaction mixture of 2a and 2b with addition of isopropylamine in methanol resulting in 4 in 42% yield.
Attempts to resolve 4 in acetonitrile with lipase A from Candida antarctica (CALA) as the catalyst and vinyl butanoate as the acyl donor were performed with an E-value of 1.8 (Scheme 4).We have previously had success with using CALA as the catalyst in the kinetic resolution of secondary alcohols with two large groups connected to the stereo centre, which atenolol also has [21].But the amine group in 4 seems to be the problem, since the amide (s) 4d may be formed in addition to the ester (s) 4b, see the chromatogram from the reaction in Figure 4.The column used did not allow sufficient separation of the two enantiomers of the butanoic ester (S/R)-3 to measure the enantiopurity of the ester.In order to verify the enantiomer formed, the ester was hydrolyzed and identified as the (S)-3 enantiomer.We have previously obtained a yield of 16% of (R)-2a, and the E-value of the kinetic resolution of 2a was >200.In our previous article, we also presented data of the S-enantiomer of the chlorohydrin, (S)-2a [18].

Synthesis of Racemic Atenolol (4) and Attempt to Resolve 4 with CALA
Another attempt to synthesizing enantiopure (S)-atenolol was performed in a kinetic resolution of racemic atenolol (4) by use of lipase A from Candida antarctica.Racemic 4 was produced directly from the reaction mixture of 2a and 2b (without the ring opening of 2b with lithium chloride) with addition of isopropylamine in methanol resulting in 4 in 42% yield (Scheme 3).

Synthesis of Racemic Atenolol (4) and Attempt to Resolve 4 with CALA
Another attempt to synthesizing enantiopure (S)-atenolol was performed in a kinetic resolution of racemic atenolol (4) by use of lipase A from Candida antarctica.Racemic 4 was produced directly from the reaction mixture of 2a and 2b (without the ring opening of 2b with lithium chloride) with addition of isopropylamine in methanol resulting in 4 in 42% yield (Scheme 3).Scheme 3. Atenolol (4) was prepared directly from the reaction mixture of 2a and 2b with addition of isopropylamine in methanol resulting in 4 in 42% yield.
Attempts to resolve 4 in acetonitrile with lipase A from Candida antarctica (CALA) as the catalyst and vinyl butanoate as the acyl donor were performed with an E-value of 1.8 (Scheme 4).We have previously had success with using CALA as the catalyst in the kinetic resolution of secondary alcohols with two large groups connected to the stereo centre, which atenolol also has [21].But the amine group in 4 seems to be the problem, since the amide (s) 4d may be formed in addition to the ester (s) 4b, see the chromatogram from the reaction in Figure 4.
Scheme 3. Atenolol (4) was prepared directly from the reaction mixture of 2a and 2b with addition of isopropylamine in methanol resulting in 4 in 42% yield.
Attempts to resolve 4 in acetonitrile with lipase A from Candida antarctica (CALA) as the catalyst and vinyl butanoate as the acyl donor were performed with an E-value of 1.8 (Scheme 4).We have previously had success with using CALA as the catalyst in the kinetic resolution of secondary alcohols with two large groups connected to the stereo centre, which atenolol also has [21].But the amine group in 4 seems to be the problem, since the amide (s) 4d may be formed in addition to the ester (s) 4b, see the chromatogram from the reaction in Figure 4.The ee of the unreacted (S)-atenolol was 4% (ees ((S)-atenolol, (S)-4)) and the ee for the product ester (R)-4b was 27% (eep).The retention times were assigned due to the known stereo preference of CALA [21], tR (S)-4 = 18.36 min and tR (R)-4 = 28.97min, RS = 2.72, tR (S)-4b = 25.04 min and tR (R)-4b = 33.87min, Chiralcel OD-H column with a gradient mobile phase i-PrOH/n-hexane; 9:95 (0 min)-10:90 (10 min)-60:40 (80 min), flow 0.5 mL/min (Figure 4).
These results show that CALA exhibits low selectivity for the enantiomers atenolol (4).The racemic compound with tR = 14.15 min and tR = 16.94 min is anticipated to be the enantiomers of the amide product 4d (Scheme 4).Chałupka (Sikora) et al. claim that they have succeeded in resolving racemic atenolol with Candida rugosa lipase with vinyl acetate as the acyl donor and ionic liquids/toluene as the solvent yielding 94% ee of the (S)atenolol acetate [11].However, the absolute configuration of this product has not been confirmed.
These results show that CALA exhibits low selectivity for the enantiomers of atenolol (4).The racemic compound with tR = 14.15 min and tR = 16.94 min is anticipated to be the enantiomers of the amide product 4d (Scheme 4).Chałupka (Sikora) et al. claim that they have succeeded in resolving racemic atenolol with Candida rugosa lipase with vinyl acetate as the acyl donor and ionic liquids/toluene as the solvent yielding 94% ee of the (S)atenolol acetate [11].However, the absolute configuration of this product has not been confirmed.
These results show that CALA exhibits low selectivity for the enantiomers of atenolol (4).The racemic compound with t R = 14.15 min and t R = 16.94 min is anticipated to be the enantiomers of the amide product 4d (Scheme 4).Chałupka (Sikora) et al. claim that they have succeeded in resolving racemic atenolol with Candida rugosa lipase with vinyl acetate as the acyl donor and ionic liquids/toluene as the solvent yielding 94% ee of the (S)-atenolol acetate [11].However, the absolute configuration of this product has not been confirmed.

Chemicals
All chemicals are commercially available and of analytic grade.The chemicals were bought from Sigma-Aldrich Norway (Oslo, Norway).HPLC grade solvents were used for HPLC analyses.Dry solvents (tetrahydrofuran and acetonitrile) were prepared with a solvent purifier, MBraun MDSPS800 (München, Germany).n-Hexane was dried manually by adding molecular sieves (4Å) to the solvent 24 h before use.Molecular sieves (1/8 pellets, pore diameter 4 Å) were placed in a porcelain dish and dried at 1000 • C for 24 h and kept in a desiccator thereafter.
The enzymatic reactions were performed in a New Brunswick G24 Environmental Incubator Shaker from New Brunswick Co. Inc. (Edison, NJ, USA) or in an Infors Minitron (Infors AG, Bottmingen, Switzerland).

High-Performance Liquid Chromatography (HPLC)
Achiral HPLC analyses were performed on an Agilent 1290 system equipped with an auto injector (4 µL), and detection was performed by a diode array detector (DAD, l = 254 nm) (Santa Clara, CA, USA).All separations of 1, 2a and 2b were performed on an Agilent Zorbax Eclipse XBD-C18 column (150 mm L × 4.6 mm i.d., 5 µm particle size) from Matriks (Oslo, Norway) with an isocratic eluent (H 2 O:MeCN, 75:25) over 5 min with a flow of 1.0 mL/min, which produced t R 1b = 1.86 min, t R 2b = 3.04 min and t R 2a = 3.32 min.When stoichiometric amount of sodium hydroxide was used also, the byproduct 2c was seen, then a linear gradient mobile phase composition of H 2 O and MeCN (75:25)-(65:35) over 20 min with 0.5 mL/min flow was the method on the Zorbax Eclipse XDB C18-column, producing t R 1 = 3.03 min, t R 2a = 12.06 min, t R 2b = 9.59 min and the by-product t R 2c = 13.38 min.
LC-MS analysis of 2c was performed on an ACQUITY UPLC BEH C18 column (100 mm L × 2.1 mm i.d., 1.7 µm particle size) from Waters TM (Waters Norway, Oslo, Norway) with isocratic mobile phase (H 2 O:MeCN, 30:70) with 1% formic acid and a flow of 0.2 mL/min resulting in a mass of 382.04 g/mol which corresponds to C 19 H 21 N 2 O 5 Na.The calculated mass of 2c is 358.39 g/mol, the formula for which is C 19 H 22 N 2 O 5 .

NMR Analyses
NMR analyses were recorded on a Bruker 400 MHz Avance III HD instrument equipped with a 5 mm SmartProbe Z-gradient probe operating at 400 MHz for 1 H and 100 MHz for 13 C, respectively, or on a Bruker 600 MHz Avance III HD instrument equipped with a 5 mm cryogenic CP-TCI Z-gradient probe operating at 600 MHz for 1 H and 150 MHz for 13 C (Bruker, Rheinstetten, Germany).Chemical shifts are in ppm relative to TMS and coupling constants are in hertz (Hz).

Mass Spectroscopy Analyses
Accurate mass determination in positive and negative mode was performed on a "Synapt G2-S" Q-TOF instrument from Waters™ (Waters Norway, Oslo, Norway).Samples were ionized by the use of ASAP probe (APCI).Calculated exact mass and spectra processing were performed by Waters™ Software (Masslynxs V4.1 SCN871).

Infrared Spectroscopy Analyses
Infrared spectroscopy was performed on a NEXUS FT-IR model 470 instrument from Thermo Nicolet Corporation (Madison, WI, USA).

Specific Rotation Analyses
Specific rotation was determined on a PerkinElmer Model 341 Polarimeter (Waltham, MA, USA), with a cell of 10 cm length, λ 589 nm.

Assignment of Absolute Configurations
Absolute configuration of the faster reacting enantiomer in lipase catalyzed resolution was determined by the known enantioselectivity of CALA [21] and CALB [22] and by comparing the elution orders of the atenolol building block enantiomers and drug enantiomer with previous analyses of similar α-halogenated 1-(4-benzyloxy)phenyl)ethanols on the Daicel Chiralcel OD-H column.In general, the R-enantiomers are the most retained [23].

Conclusions
A four-step synthesis of (S)-atenolol ((S)-4) in >99% ee has been performed, starting from 2-(4-hydroxyphenyl)-acetamide (1).The base catalyzed deprotonation of the starting material avoided formation of the by-product (2c), thus resulting in an overall yield of the racemic chlorohydrin 2a to 52%.This is an improvement from our previous reported yield of 2a of 22%.CALB catalyzed kinetic resolution of 2a gave the enantiopure (R)-2a in >99% ee with 32% yield.CALB catalyzed kinetic resolution of chlorohydrin 2a is an efficient method to obtain enantiopure building blocks for beta-blockers; however, the yield of this enzymatic step is limited to 50%.Our new protocol to achieve enantiopure (S)-atenolol

Figure 2 .
Figure 2. LC-MS analysis of the by-product 2c showed a molecular mass of 382.047 g/mol, which corresponds to C19H22N2O5Na.The calculated mass of 2c is 358.389189g/mol, C19H22N2O5, which results in the structure of 2c.The reaction mixture showed in the chromatogram in Figure 1 was analyzed on an AQUITY UPLC BEH C18-column with isocratic mobile phase composition of water and acetonitrile (30:70) with 1% formic acid and a flow of 0.2 mL/min.

Figure 2 .
Figure 2. LC-MS analysis of the by-product 2c showed a molecular mass of 382.047 g/mol, which corresponds to C19H22N2O5Na.The calculated mass of 2c is 358.389189g/mol, C19H22N2O5, which results in the structure of 2c.The reaction mixture showed in the chromatogram in Figure 1 was analyzed on an AQUITY UPLC BEH C18-column with isocratic mobile phase composition of water and acetonitrile (30:70) with 1% formic acid and a flow of 0.2 mL/min.When two equivalents of epichlorohydrin were added to a solution containing phenol 1 and catalytical amounts of sodium hydroxide, the by-product 2c was not observed after 4 hours of reaction.A proposed mechanism for the reaction of 1 to 2a and 2b with the regeneration of the base is shown in Scheme 2. Phenol 1 is deprotonated to form phenoxide 1′ which can attack epichlorohydrin at carbon 1 or 3 (reaction mechanism a and b, respectively) resulting in phenoxide 2a′ and the epoxide 2b.Phenoxide 2a′ may react in two ways: by deprotonating a water molecule forming chlorohydrin 2a, thus regenerating

Figure 2 .
Figure 2. LC-MS analysis of the by-product 2c showed a molecular mass of 382.047 g/mol, which corresponds to C 19 H 22 N 2 O 5 Na.The calculated mass of 2c is 358.389189g/mol, C 19 H 22 N 2 O 5 , which results in the structure of 2c.The reaction mixture showed in the chromatogram in Figure 1 was analyzed on an AQUITY UPLC BEH C18-column with isocratic mobile phase composition of water and acetonitrile (30:70) with 1% formic acid and a flow of 0.2 mL/min.

Scheme 2 .
Scheme 2. Reaction mechanism for the sodium hydroxide catalyzed reaction of phenol 1 forming chlorohydrin 2a and epoxide 2b.Phenol 1 is deprotonated forming alkoxide 1′ which can attack epichlorohydrin at carbon 1 and 3 (reaction mechanism a and b, respectively) resulting in phenoxide 2a′ and epoxide 2b.Phenoxide 2a′ reacts in two ways: by protonation from water forming chlorohydrin 2a, thus regenerating the base (reaction mechanism c), and by an intramolecular SN2-like reaction forming epoxide 2b (reaction mechanism d).

Scheme 2 .
Scheme 2. Reaction mechanism for the sodium hydroxide catalyzed reaction of phenol 1 forming chlorohydrin 2a and epoxide 2b.Phenol 1 is deprotonated forming alkoxide 1 ′ which can attack epichlorohydrin at carbon 1 and 3 (reaction mechanism a and b, respectively) resulting in phenoxide 2a ′ and epoxide 2b.Phenoxide 2a ′ reacts in two ways: by protonation from water forming chlorohydrin 2a, thus regenerating the base (reaction mechanism c), and by an intramolecular S N 2-like reaction forming epoxide 2b (reaction mechanism d).
Scheme 4. Kinetic resolution of 4 in dry acetonitrile with lipase A from Candida antarctica (CALA) as the catalyst and vinyl butanoate as acyl donor at 30 °C and 200 rpm showing several acetylation products.

Scheme 4 .
Scheme 4. Kinetic resolution of 4 in dry acetonitrile with lipase A from Candida antarctica (CALA) as the catalyst and vinyl butanoate as acyl donor at 30 • C and 200 rpm showing several acetylation products.