The Roles of Circadian Clock Genes in Plant Temperature Stress Responses

Plants monitor day length and memorize changes in temperature signals throughout the day, creating circadian rhythms that support the timely control of physiological and metabolic processes. The DEHYDRATION-RESPONSE ELEMENT-BINDING PROTEIN 1/C-REPEAT BINDING FACTOR (DREB1/CBF) transcription factors are known as master regulators for the acquisition of cold stress tolerance, whereas PHYTOCHROME INTERACTING FACTOR 4 (PIF4) is involved in plant adaptation to heat stress through thermomorphogenesis. Recent studies have shown that circadian clock genes control plant responses to temperature. Temperature-responsive transcriptomes show a diurnal cycle and peak expression levels at specific times of throughout the day. Circadian clock genes play essential roles in allowing plants to maintain homeostasis by accommodating temperature changes within the normal temperature range or by altering protein properties and morphogenesis at the cellular level for plant survival and growth under temperature stress conditions. Recent studies revealed that the central oscillator genes CIRCADIAN CLOCK ASSOCIATED 1/LATE ELONGATED HYPOCOTYL (CCA1/LHY) and PSEUDO-RESPONSE REGULATOR5/7/9 (PRR5/7/9), as well as the EVENING COMPLEX (EC) genes REVEILLE4/REVEILLE8 (REV4/REV8), were involved in the DREB1 pathway of the cold signaling transcription factor and regulated the thermomorphogenesis gene PIF4. Further studies showed that another central oscillator, TIMING OF CAB EXPRESSION 1 (TOC1), and the regulatory protein ZEITLUPE (ZTL) are also involved. These studies led to attempts to utilize circadian clock genes for the acquisition of temperature-stress resistance in crops. In this review, we highlight circadian rhythm regulation and the clock genes involved in plant responses to temperature changes, as well as strategies for plant survival in a rapidly changing global climate.


Introduction
Every organism living on the rotating Earth has evolved a circadian clock that regulates biological processes on the basis of environmental signals, which change according to the time of day [1].The circadian clock is a molecular timing device that generates the physiological and biological rhythms of numerous developmental processes in 24 h cycles in most living organisms [2].Circadian rhythms are endogenously generated and selfmaintaining, allowing them to preserve robust and precise timing across a wide range of physiological temperatures [3,4].The clock is a significant regulator of plant life, linked to various signaling and metabolic pathways that promote development and environmental adaptation responses [5][6][7].In many plant species, the correct synchronization of the clock with the environment contributes to fitness, ensuring survival in addition to optimal growth and performance under fluctuating environmental conditions.
Changes in temperature throughout the day act as entrainment signals that provide time information to plants, supporting the creation of a rhythm [8].Temperature also Plant clock systems are complex genetic circuits containing multiple inhibitory feedback loops [11].The transcriptional regulatory network model system that regulates circadian rhythm has been widely studied in Arabidopsis thaliana.The feedback loop of the main oscillator consists of CCA1, LATE ELONGATED HYPOCOTYL (LHY), TIMING OF CAB EXPRESSION 1 (TOC1) (also known as PSEUDO-RESPONSE REGULATOR1, PRR1), the MYB transcription factor LUX ARRHYTHMO (LUX), and EVENING COMPLEX (EC), as well as the transcriptional regulatory factors EARLY FLOWERING 3 (ELF3) and ELF4.In this central oscillator feedback loop, CCA1 and LHY show peak expressions in the morning and repress the transcription of TOC1; in turn, TOC1 regulates CCA1 and LHY expression in the late evening.Additionally, CCA1 and LHY activate the PRR7 and PRR9 genes, which then repress CCA1 gene transcription in a temporal sequence.TOC1 represses the transcription of EC components, PRRs, and GIGANTEA (GI).Other major components of the central oscillator include GI, ZTL, and PRR3.LHY and CCA1 repress GI transcription in the early morning, whereas GI transcription peaks in the middle of the day [12,13].

Clock Genes Related to Temperature Compensation Mechanisms
The operation and maintenance of biological systems with precise responses to environmental changes is essential for plant survival and optimal growth.Plant circadian clocks have a molecular buffering system that allows for the maintenance of robust circadian rhythms, rendering the clock output pathway insensitive to physiological ranges of temperature fluctuations; this phenomenon is known as temperature compensation [38].Temperature compensation mechanisms are highly conserved and considered essential properties of circadian clocks within living organisms.Studies with model plants indicated that a group of central clock components is involved in temperature compensation processes [39,40].Arabidopsis plants lacking PRR7 and PRR9 could not maintain circadian rhythms in response to warm-cold cycles, suggesting that PRR proteins are crucial for the temperature entrainment of the clock [41].GI is also important for the CCA1/LHY-mediated buffering of robust circadian rhythms; it serves as a potential regulator of temperature compensation [40].Temperature compensation mechanisms are highly conserved and considered essential properties of circadian clocks within living organisms [26,42,43].
Studies to date have shown that the ZTL and HSP protein groups, as well as the FLOWERING BASIC HELIX-LOOP-HELIX 1 (FBH1) and HEAT SHOCK FACTOR (HSF) protein groups, are crucial components of the plant circadian clock response to warm temperatures [10].The overproduction of FBH1 alters the clock period by regulating the CCA1 response to temperature changes [44].The heat-responsive HsfB2b transcription factor also inhibits PRR7 transcription by binding to the promoter under elevated temperature conditions [45].In contrast, a lack of HsfB2b causes the circadian period to be shortened under the same temperature conditions.These findings suggest that circadian clock genes transmit temperature signals and may affect resistance to temperature stress through mechanisms similar to other abiotic stress responses.Although it is unclear whether clock components operate independently or cooperatively through an integrated signaling network during the temperature compensation process, the relationships among clock genes and temperature response genes have attracted the attention of scientists interested in establishing temperature stress resistance in plants through the use of clock genes.

Cold Stress Response in Plants
In Arabidopsis, the DEHYDRATION-RESPONSE ELEMENT-BINDING PROTEIN 1/C-REPEAT BINDING FACTORs (DREB1/CBFs) were identified as major transcription factors involved in cold-responsive gene expression [46,47].These DREB1/CBFs are APETALA2/ETHYLENE-RESPONSIVE FACTOR (AP2/ERF) transcription factors, which bind to DEHYDRATION-RESPONSE ELEMENT (DRE) and act as master regulators in coldinduced gene expression.There are three DREB1/CBF genes in Arabidopsis: DREB1A/CBF3, DREB1B/CBF1, and DREB1C/CBF2.These genes are arranged adjacent to each other in the Arabidopsis genome in the following order: DREB1B, DREB1A, and DREB1C.Arabidopsis mutant plants with all three genes disrupted exhibit severely impaired freezing tolerance.All three genes are rapidly induced by cold stress, and the induction of the DREB1 gene triggers a cold-responsive transcription cascade, leading to the expression of multiple cold-inducible genes.Therefore, research concerning the DREB1 expression mechanism in response to cold stress is important for understanding plant perception and response.Members of the CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR (CAMTA) protein family are transcriptional activators that trigger the expression of CBF and positively regulate freezing tolerance in Arabidopsis.CAMTA3 and CAMTA5 regulate CBF1 and CBF2 expression in response to rapid temperature decreases but not in response to gradually decreasing temperatures.

Cold Signaling Pathways Involving Clock-Related Transcription Factors
Many cold-induced genes, including DREB1A, are expressed at low levels around noon under normal temperature conditions [48,49].Mutations in genes encoding PRR9, PRR7, and PRR5-the central circadian oscillator-enhanced the expression of the DREB1 gene and its downstream genes [14,50].Additionally, the cold-induced expression of the DREB1 gene was significantly reduced in double-mutant plants of CCA1 and LHY.These results indicate that many cold-induced genes, including DREB1A, are regulated by clock genes and thus likely to affect the plant response to low temperature.
In addition to CCA1 and LHY, regulatory factors include REVEILLE4/LHY-CCA1-LIKE1 (RVE4/LCL1) and RVE8/LCL5, which regulate DREB1 expression by binding to the EVENING ELEMENT (EE) in the DREB1 promoter region (Figure 1).EE directly binds to promoters in the circadian clock and activates the expression of evening genes repressed by CCA1 and LHY [51][52][53].Under low-temperature conditions, RVE4 and RVE8 bind directly to the DREB1 promoter and function as transcriptional activators.The function of RVE6 overlaps with the functions of RVE3 and RVE5, but RVE6 conditionally activates DREB1 expression only when the activities of RVE4 and RVE8 are lost.Additionally, the RVE4 and RVE8 proteins are rapidly and reversibly transported from the cytoplasm to the nucleus in response to cold stress, thus activating the expression of DREB1.Under normal stress-free conditions, CCA1 and LHY proteins bind to the DREB1 promoter and act as transcriptional repressors of DREB1 expression.In particular, they are rapidly degraded under cold stress conditions to induce DREB1 expression.However, some CCA1 and LHY proteins are not degraded; they exist at low levels under cold stress conditions and bind to RVE1 and RVE2 to suppress the expression of the cold-induced gene DREB1 [54].CCA1 and LHY bind to RVE4/RVE8 under cold stress conditions and indirectly increase the expression of DREB1 while directly regulating the expression of COLD-REGULATED/RESPONSIVE TO DEHYDRATION (COR/RD) genes without the mediation of DREB1/CBFs (Figure 1).These findings indicate that clock-related transcription factors play an essential role in triggering the expression of cold-induced genes.Studies on their mechanisms will reveal the complex relationships of plant biological clocks with cold stress responses, resulting in breeding materials that can be used to develop cold-stress-resistant crops.

Clock Genes Involved in Detecting Temperature Decreases
EC, composed of the DNA-binding proteins LUX, ELF3, and ELF4, is a transcriptional repression complex that constitutes a crucial component of the plant circadian clock.EC regulates temperature-dependent plant growth in the normal temperature range.EC can act as a temperature sensor through its stronger DNA binding at 4 °C and weaker binding at 27 °C; these binding interactions are regulated by ELF4.Moreover, ELF4 can move from shoots to roots; low temperature enhances this movement [56].ELF3 is a scaffold protein and vital component of temperature-sensing machinery.Polyglutamine repeats in the prion-like domain of ELF3 are required for its function as a temperature sensor [57].However, low-temperature detection by ELF3 below 12° C has not yet been demonstrated.The post-translational regulation of clock-related MYB, such as the nuclear accumulation of RVE4 and RVE8 and the degradation of CCA1 and LHY, occurs in response to cold stress.Detailed mechanistic analyses of the cold-responsive regulation of circadian components may lead to the elucidation of temperature-sensing systems.

Responsive Transcriptomes Revealing a Diurnal Response to Heat Stress in Plants
In recent decades, studies have demonstrated that circadian clock genes are also involved in plant responses to heat stress [58,59].Although the relationship between the low-temperature stress response and the circadian clock has been widely documented, the circadian clock contribution to the heat stress response remains largely unknown.Research concerning plant clock genes involved in heat stress adaptation is ongoing [60][61][62][63].
Based on transcriptome experiments, up to 50% of the genes responsive to heat, cold, salinity, osmotic pressure, or water deprivation exhibit circadian rhythmicity in Arabidopsis [17, 64,65].Heat stress also significantly perturbs the transcriptome, ~70% of which displays a time-of-day (i.e., ZT1 or ZT6) response [66].Enriched gene ontology functional categories related to high-temperature stress include many of the known heat shock transcription factors (HSFs) and heat shock proteins (HSPs) [67].Whereas the

Clock Genes Involved in Detecting Temperature Decreases
EC, composed of the DNA-binding proteins LUX, ELF3, and ELF4, is a transcriptional repression complex that constitutes a crucial component of the plant circadian clock.EC regulates temperature-dependent plant growth in the normal temperature range.EC can act as a temperature sensor through its stronger DNA binding at 4 • C and weaker binding at 27 • C; these binding interactions are regulated by ELF4.Moreover, ELF4 can move from shoots to roots; low temperature enhances this movement [56].ELF3 is a scaffold protein and vital component of temperature-sensing machinery.Polyglutamine repeats in the prion-like domain of ELF3 are required for its function as a temperature sensor [57].However, low-temperature detection by ELF3 below 12 • C has not yet been demonstrated.The post-translational regulation of clock-related MYB, such as the nuclear accumulation of RVE4 and RVE8 and the degradation of CCA1 and LHY, occurs in response to cold stress.Detailed mechanistic analyses of the cold-responsive regulation of circadian components may lead to the elucidation of temperature-sensing systems.

Responsive Transcriptomes Revealing a Diurnal Response to Heat Stress in Plants
In recent decades, studies have demonstrated that circadian clock genes are also involved in plant responses to heat stress [58,59].Although the relationship between the low-temperature stress response and the circadian clock has been widely documented, the circadian clock contribution to the heat stress response remains largely unknown.Research concerning plant clock genes involved in heat stress adaptation is ongoing [60][61][62][63].
Based on transcriptome experiments, up to 50% of the genes responsive to heat, cold, salinity, osmotic pressure, or water deprivation exhibit circadian rhythmicity in Arabidopsis [17, 64,65].Heat stress also significantly perturbs the transcriptome, ~70% of which displays a time-of-day (i.e., ZT1 or ZT6) response [66].Enriched gene ontology functional categories related to high-temperature stress include many of the known heat shock transcription factors (HSFs) and heat shock proteins (HSPs) [67].Whereas the differentially expressed genes (DEGs) of HSFs and HSPs are primarily upregulated in response to heat stress, DEGs of categories including response to abscisic acid, alcohol, and lipids are downregulated in response to heat stress conditions.Experimental analyses showed that these DEGs were enriched at the time points ZT1 and ZT6.For example, the induction of HSFA3 in response to heat stress was greater at ZT6 relative to the wild type, suggesting that HSFA3 is more sensitive to heat stress in the early afternoon.Intriguingly, the induction of HSFA3 was strongly enhanced in cca1lhy and prr7prr9 mutants at 37 • C relative to 22 • C, suggesting that the clock also plays an important role in modulating the transcriptional response of HSFA3 to high-temperature stress.
Additionally, CCA1, PRR7, and PRR9 were upregulated under high-temperature treatment, whereas LHY was downregulated.These four components, which are related to the regulation of the low-temperature stress response, play significant roles in the heat stress-responsive transcriptome.Recently, a targeted study also suggested a role for the evening-expressed clock components TOC1 and PRR5 in gating the molecular responses of select genes to warm temperature or high ambient temperature [60].

Clock Genes Involved in Thermomorphogenesis
The strategy of adaptation to ambient temperature stress allows plants to maintain biological activity at an optimal level even under fluctuating temperature conditions [39].When A. thaliana is exposed to high temperatures during its growth period, there are increases in hypocotyl elongation and petiole extension; moreover, it forms small, thin leaves.These characteristics are known as thermomorphogenesis, and the plant moves the apical meristem away from the hot soil surface to promote evaporative cooling of leaves [68].Thermomorphogenic adaptation responses help plants to efficiently manage body heat dissipation and leaf cooling, thereby optimizing growth and fitness in warm climates [69,70].Thermomorphogenesis is mainly mediated by PHYTOCHROME INTER-ACTING FACTOR4 (PIF4), a basic helix-loop-helix transcription factor that promotes stem elongation [71].PIF4 is a central growth regulator that directly activates several auxin biosynthesis genes, including YUCCA 8 (YUC8), to increase the endogenous auxin level; this increase promotes hypocotyl elongation and hyponastic leaf growth [72,73].PIF4 is also controlled by diverse environmental signals and endogenous programs.Warm temperatures increase PIF4 expression, and the pif4-null mutant is defective in the growth response to warm temperature [74,75].Furthermore, PIF4 RNA expression is controlled by the circadian clock [76,77].
Both TOC1 and PRR5, two evening element clock proteins, directly interact with PIF4; they inhibit PIF4-mediated growth promotion by inhibiting its transcriptional activity (Figure 2) [60,78].The TOC1-PIF4 interaction can repress PIF4 activity without altering the ability of PIF4 to bind to the YUC8, IAA19, and IAA29 promoters.TOC1 directly represses PIF4's ability to activate target gene expression.Because PIF4 is required for thermomorphogenesis, including hypocotyl growth responses to warm temperature, it is possible that the TOC1-PIF4 interaction suppresses thermoresponsive growth.In an experimental analysis, the hypocotyls of toc1-2 mutants were more elongated by transient high-temperature exposure compared with hypocotyls of the wild type, and TOC1-overexpressed (TOC1-OX) plants were completely insensitive to high-temperature treatment.PRR5-OX plants, but not other PRR family members (PRR3, PRR7, or PRR9), were also insensitive to warm temperature.Consistent with the thermo-insensitive hypocotyl growth of TOC1-OX, the warm-temperature activation of PIF4 target genes YUC8, IAA19, and IAA29 was abolished in TOC1-OX.However, the expression of the early high temperature-induced gene HEAT SHOCK PROTEIN 70 (HSP70) was induced at normal levels by warm temperatures in TOC1-OX.TOC1 and PRR5 inhibit the warm-temperature activation of PIF4 target genes through direct repression of PIF4 protein activity.Plant architectural adaptation to warm temperature, mediated by PIF4, enhances evaporative leaf cooling and thus may improve plant survival under heat stress [69,75].This adaptive effect was considerably reduced in the pif4 mutant, whereas PIF4-OX plants were more tolerant to heat stress than the wild type.Consistent with the TOC1 inhibition of PIF4 activity, TOC1-OX plants were less thermotolerant, but the toc1prr5 double mutant showed more thermotolerance than wild-type plants when grown under warm temperatures.When grown under normal temperatures, the TOC1-OX and toc1prr5 plants showed similar sensitivity to heat stress.These results indicate that adaptive thermotolerance requires PIF4 activation and a low level of TOC1, suggesting that PIF4 activation by daytime warm temperatures, allowed by troughs in the level of TOC1, enhances plant survival during heat stress.
PIF4-OX plants were more tolerant to heat stress than the wild type.Consistent with the TOC1 inhibition of PIF4 activity, TOC1-OX plants were less thermotolerant, but the toc1prr5 double mutant showed more thermotolerance than wild-type plants when grown under warm temperatures.When grown under normal temperatures, the TOC1-OX and toc1prr5 plants showed similar sensitivity to heat stress.These results indicate that adaptive thermotolerance requires PIF4 activation and a low level of TOC1, suggesting that PIF4 activation by daytime warm temperatures, allowed by troughs in the level of TOC1, enhances plant survival during heat stress., which then activates auxin biosynthesis genes including YUCCA8 (YUC8), promoting hypocotyl growth.However, in the evening and early night, TIMING OF CAB EXPRESSION 1 (TOC1) accumulates at high levels and directly inhibits PIF4, suppressing thermomorphogenesis [60].Additionally, warm temperatures enhance the accumulation of the chaperone GIGANTEA (GI), which thermostabilizes the DELLA protein REPRESSOR OF GA1-3 (RGA).RGA stabilized by GI negatively regulates PIF4 protein during thermomorphogenesis [61].
Warm temperatures enhance the accumulation of the chaperone GIGANTEA (GI), which thermostabilizes the DELLA protein REPRESSOR OF GA1-3 (RGA) under long-day conditions, thereby attenuating PIF4-mediated thermomorphogenesis.Mutant studies provided insights concerning the role of GI in various abiotic stresses, as well as various growth and developmental processes.GI interacts with multiple PIF proteins that mediate diverse facets of light-signaling events and accompanying photomorphogenic responses in plants [79].GI attenuates PIF4 function by stabilizing DELLA proteins, which function as negative regulators of PIF proteins [80], during thermomorphogenesis.The chaperone activity of GI protects substrate proteins, such as ZTL and RGA, from the ubiquitin-proteasome pathway [81].HSP90 is a representative molecular chaperone that stabilizes a broad spectrum of cellular proteins in plants under high-temperature stress [82].GI functions along with HSP90 during the maturation of ZTL proteins [83].In an experimental analysis, HSP90 and GI accumulation under warm temperatures led to the intrinsic chaperone activity of GI, supporting the thermostabilization of the RGA protein during hypocotyl thermomorphogenesis [84].These results indicate that the GI-mediated integration of photoperiodic and temperature information shapes thermomorphogenic rhythms, which enable plants to adapt to fluctuations in day length and temperature during seasonal transitions (Figure 2).

Circadian Clock Genes Involved in Heat Resistance
ZTL increases thermotolerance through a protein expression system [10].In contrast to thermomorphogenesis, ZTL appears to regulate different high-temperature responses through distinct mechanisms; the thermotolerance defect in ztl mutants is not restored by TOC1 and PRR5 mutations [85].Increased ZTL expression at high temperatures may promote plant survival under high-temperature stress.ZTL, an HSP90 client protein, improves heat resistance by resolving high-temperature-induced protein ag- , which then activates auxin biosynthesis genes including YUCCA8 (YUC8), promoting hypocotyl growth.However, in the evening and early night, TIMING OF CAB EXPRESSION 1 (TOC1) accumulates at high levels and directly inhibits PIF4, suppressing thermomorphogenesis [60].Additionally, warm temperatures enhance the accumulation of the chaperone GIGANTEA (GI), which thermostabilizes the DELLA protein REPRESSOR OF GA1-3 (RGA).RGA stabilized by GI negatively regulates PIF4 protein during thermomorphogenesis [61].
Warm temperatures enhance the accumulation of the chaperone GIGANTEA (GI), which thermostabilizes the DELLA protein REPRESSOR OF GA1-3 (RGA) under long-day conditions, thereby attenuating PIF4-mediated thermomorphogenesis.Mutant studies provided insights concerning the role of GI in various abiotic stresses, as well as various growth and developmental processes.GI interacts with multiple PIF proteins that mediate diverse facets of light-signaling events and accompanying photomorphogenic responses in plants [79].GI attenuates PIF4 function by stabilizing DELLA proteins, which function as negative regulators of PIF proteins [80], during thermomorphogenesis.The chaperone activity of GI protects substrate proteins, such as ZTL and RGA, from the ubiquitin-proteasome pathway [81].HSP90 is a representative molecular chaperone that stabilizes a broad spectrum of cellular proteins in plants under high-temperature stress [82].GI functions along with HSP90 during the maturation of ZTL proteins [83].In an experimental analysis, HSP90 and GI accumulation under warm temperatures led to the intrinsic chaperone activity of GI, supporting the thermostabilization of the RGA protein during hypocotyl thermomorphogenesis [84].These results indicate that the GI-mediated integration of photoperiodic and temperature information shapes thermomorphogenic rhythms, which enable plants to adapt to fluctuations in day length and temperature during seasonal transitions (Figure 2).

Circadian Clock Genes Involved in Heat Resistance
ZTL increases thermotolerance through a protein expression system [10].In contrast to thermomorphogenesis, ZTL appears to regulate different high-temperature responses through distinct mechanisms; the thermotolerance defect in ztl mutants is not restored by TOC1 and PRR5 mutations [85].Increased ZTL expression at high temperatures may promote plant survival under high-temperature stress.ZTL, an HSP90 client protein, improves heat resistance by resolving high-temperature-induced protein aggregation through polyubiquitination-mediated protein degradation [10].HSP90 positively controls thermomorphogenesis by stabilizing the auxin receptor TRANSFORT INHIBITOR RESPONSE 1 (TIR1) [84].HSP90 is associated with GI and regulates the maturation of ZTL, which is required for the nighttime degradation of TOC1 and PRR5.Overall, HSP90 regulates thermomorphogenesis through the ZTL-TOC1/PRR5 signaling module and the TIR1 auxin receptor.The ztl-105 mutant exhibited reduced thermotolerance, accompanied by a decline in polyubiquitination and an increase in protein aggregate formation.ZTL and its interacting partner HSP90 were cofractionated with insoluble aggregates under heat stress, indicating that ZTL contributes to the thermoresponsive protein quality control machinery.Thus, ZTL-mediated protein quality control contributes to the thermal stability of the circadian clock.

Conclusions
In nature, plants frequently encounter temperature changes within various temperature regimes, and they are equipped with defense mechanisms to adapt to temperature stimuli.In the model plant A. thaliana, which has an optimal growth temperature of 22 • C, normal growth is possible in the range of 16-28 • C. Temperatures below 16 • C or above 29 • C are perceived as stress, leading to severely reduced growth, distorted plant structure and morphology, and decreased seed production [86].Circadian clock genes play essential roles in allowing plants to maintain homeostasis through the accommodation of temperature changes in the normal temperature range or alterations of protein properties and morphogenesis at the cellular level.The molecular mechanism that buffers the clock in response to changes in cellular metabolism has been explored in various plant species and is considered an essential function for maintaining plant growth, development, and survival under extreme conditions through the stabilization of the clock cycle [26,42,87].The clock genes also directly influence key crop traits [88].Additionally, considering that clock genes are involved in responses to various environmental stresses such as salt and drought, studies of clock genes and expression regulation technologies are important for efforts to improve plant characteristics.Various efforts have been made to mutate clock genes to improve various crop characteristics, including RNAi and overexpression, as well as recent gene-editing techniques (Table 1) [89,90].In recent years, global warming has emerged as an important ecological problem for vegetation and crop agriculture worldwide [86]; thus, further analyses of the relationships between temperature stress response and plant growth and development are necessary to secure future plant resources and food security.

Table 1 .
Roles of circadian clock genes in response to environmental stress in different plant species.