Are Voltage Sensors Really Embedded in Muscarinic Receptors?

Unexpectedly, the affinity of the seven-transmembrane muscarinic acetylcholine receptors for their agonists is modulated by membrane depolarization. Recent reports attribute this characteristic to an embedded charge movement in the muscarinic receptor, acting as a voltage sensor. However, this explanation is inconsistent with the results of experiments measuring acetylcholine binding to muscarinic receptors in brain synaptoneurosomes. According to these results, the gating of the voltage-dependent sodium channel (VDSC) acts as the voltage sensor, generating activation of Go-proteins in response to membrane depolarization, and this modulates the affinity of muscarinic receptors for their cholinergic agonists.


Introduction
Muscarinic receptors are seven transmembrane cholinergic receptors. They possess an extracellular amino-terminus, seven membrane-traversing hydrophobic helices, and a cytoplasmic carboxyl terminus [1]. Agonists and antagonists binding to these receptors affect downstream signal transduction mechanisms, mainly by manipulating ion currents and via activation of heterotrimeric GTP-binding proteins [1,2].
Muscarinic receptors are modified by post-translational modifications affecting their binding to other proteins and lipids in the cell membrane [1]. In addition, the affinity of muscarinic receptors for their cholinergic agonists is modulated by membrane depolarization [1,2]. Recently, this characteristic has been attributed to a depolarization-induced charge movement in the muscarinic receptor. However, this explanation is inconsistent with numerous findings that are briefly presented here. Unveiling the molecular mechanism underlying the voltage-dependent affinity modulation of muscarinic receptors for their agonists is of significant importance for targeting the physiological outcome of muscarinic receptor stimulation in a variety of signal transduction mechanisms [3].

Discussion
Different affinities of muscarinic receptors for their agonists were identified four decades ago [4]. A depolarization-induced modulation of the affinity of muscarinic receptors for their cholinergic agonists was first identified in the rat brain cortex, brain stem, and atria [5]. It was further confirmed for the affinity of the M2 muscarinic receptor subtype [6]. The voltage-induced modulation of the muscarinic receptors affinity for agonists is apparently one of the common features of muscarinic receptor subtypes [4,5,7]. Recent studies attribute the voltage-dependent modulation of muscarinic receptor affinity to a voltage-induced charge movement in the muscarinic receptor [6,8]. A charge movement producing gating current was attributed to modifications in the intramolecular loops L2 and L3 of muscarinic receptors, which are implicated in the coupling of the muscarinic receptor to trimetric G-proteins [6,8]. Unlike previous reports, these studies attribute opposite effects of membrane depolarization on the affinity of muscarinic receptor subtypes M1 and M2 [5,8]. M1 is the main muscarinic receptor subtype in the brain, and the M2 muscarinic  22 Na + uptake (circles) and [ 3 H]BTX binding (squares) to pertussis toxintreated synaptoneurosomes, as a function of carbamylcholine concentration in the presence ( , ) and absence ( , ) of atropine (0.1 µ M). The specific carbamylcholine-induced binding of [ 3 H]BTX was measured in the presence of TTX (1 µ M). The non-specific carbamylcholine-induced 22 Na + uptake, both in the presence and absence of atropine, was measured in the presence of TTX (1 µ M) [15].
Muscarinic cholinergic receptor agonists dose-dependently enhanced the specific binding of [ 3 H]BTX to open VDSC in the synaptoneurosomes at resting membrane poten-    22 Na + uptake, both in the presence and absence of atropine, was measured in the presence of TTX (1 µM) [15].
The binding of certain toxins to the open configuration of VDSC either keeps the VDSC in their open configuration (e.g., Batrachotoxin (BTX) and the S-enantiomer of the cardio-tonic drug DPI) or prevents the opening of VDSC (e.g., the R-enantiomer of the  22 Na + uptake (circles) and [ 3 H]BTX binding (squares) to pertussis toxintreated synaptoneurosomes, as a function of carbamylcholine concentration in the presence ( , ) and absence ( , ) of atropine (0.1 µM). The specific carbamylcholine-induced binding of [ 3 H]BTX was measured in the presence of TTX (1 µM). The non-specific carbamylcholine-induced 22 Na + uptake, both in the presence and absence of atropine, was measured in the presence of TTX (1 µM) [15].
The binding of certain toxins to the open configuration of VDSC either keeps the VDSC in their open configuration (e.g., Batrachotoxin (BTX) and the S-enantiomer of the cardio-tonic drug DPI) or prevents the opening of VDSC (e.g., the R-enantiomer of the  22 Na + uptake (circles) and [ 3 H]BTX binding (squares) to pertussis toxintreated synaptoneurosomes, as a function of carbamylcholine concentration in the presence ( , ) and absence ( , ) of atropine (0.1 µM). The specific carbamylcholine-induced binding of [ 3 H]BTX was measured in the presence of TTX (1 µM). The non-specific carbamylcholine-induced 22 Na + uptake, both in the presence and absence of atropine, was measured in the presence of TTX (1 µM) [15].
The binding of certain toxins to the open configuration of VDSC either keeps the VDSC in their open configuration (e.g., Batrachotoxin (BTX) and the S-enantiomer of the cardio-tonic drug DPI) or prevents the opening of VDSC (e.g., the R-enantiomer of the ) of atropine (0.1 µM). The specific carbamylcholine-induced binding of [ 3 H]BTX was measured in the presence of TTX (1 µM). The non-specific carbamylcholine-induced 22 Na + uptake, both in the presence and absence of atropine, was measured in the presence of TTX (1 µM) [15].
Muscarinic cholinergic receptor agonists dose-dependently enhanced the specific binding of [ 3 H]BTX to open VDSC in the synaptoneurosomes at resting membrane potential, while Na + entry was blocked (Refs. [13,15]; Figure 1). Thus, these findings suggest that binding of cholinergic receptor agonists to muscarinic receptors induces the opening of VDSC at resting membrane potential and that the 22 Na + entry is blocked by TTX, which blocks sodium currents via VDSC in the brain (Ref. [15]; Figure 1).
A reciprocal effect of the VDSC gating on the affinity of muscarinic receptors for their agonists was identified as well [12,13]. The high affinity binding of [ 3 H]acetylcholine to muscarinic receptors in the brain synaptoneurosomes was substantially reduced under membrane depolarization [12,13], and the open configuration of VDSC was indispensable for this voltage-induced affinity change in the muscarinic receptors, even when Na + entry was blocked [12][13][14]. Thus, a depolarization-induced reduction in the high-affinity binding of [ 3 H]acetylcholine to muscarinic receptors was not measured when the opening of VDSC was prevented by the binding of the R-enantiomer of the cardiotonic drug DPI to the VDSC [12][13][14]20] (Figure 2). According to these results, the open configuration of the VDSC (but not the Na + current) was indispensable for the depolarization-induced modulation of the muscarinic receptors' affinity for cholinergic agonists, from a high affinity to a low affinity (Refs. [12][13][14]; Figure 2). The binding of antagonists to muscarinic receptors was not similarly affected by membrane depolarization or by the open configuration of the VDSC [5,12,13]. Many findings identified the involvement of muscarinic receptor coupled trimetric G-proteins in the depolarization-induced affinity modulation of muscarinic receptors [5,8,9,12,14,22]. One of these results showed that the L2 and L3 loops in the muscarinic receptor are implicated both in the receptor coupling to trimetric G-proteins and in the depolarization-induced modulation of its affinity for agonists [8,9]. These findings are in accordance with the indispensable role of the depolarization-induced activation of trimetric Go-proteins in the depolarization-induced modulation of the muscarinic receptors' affinity for their cholinergic receptor agonists [14].
The pertussis toxin (PTX)-sensitive trimetric Gi-and Go-proteins are activated by muscarinic agonists [1,2,5,12,14]. The activation of these G-proteins was measured in-situ in synaptoneurosomes by the covalent binding of a labeled GTP [14]. The covalent binding of labeled GTP to trimetric Go-proteins was induced by the binding of cholinergic recep- The binding of certain toxins to the open configuration of VDSC either keeps the VDSC in their open configuration (e.g., Batrachotoxin (BTX) and the S-enantiomer of the cardio-tonic drug DPI) or prevents the opening of VDSC (e.g., the R-enantiomer of the cardio-tonic drug DPI) [13,[17][18][19][20][21].
Muscarinic cholinergic receptor agonists dose-dependently enhanced the specific binding of [ 3 H]BTX to open VDSC in the synaptoneurosomes at resting membrane potential, while Na + entry was blocked (Refs. [13,15]; Figure 1). Thus, these findings suggest that binding of cholinergic receptor agonists to muscarinic receptors induces the opening of VDSC at resting membrane potential and that the 22 Na + entry is blocked by TTX, which blocks sodium currents via VDSC in the brain (Ref. [15]; Figure 1).
A reciprocal effect of the VDSC gating on the affinity of muscarinic receptors for their agonists was identified as well [12,13]. The high affinity binding of [ 3 H]acetylcholine to Many findings identified the involvement of muscarinic receptor coupled trimetric G-proteins in the depolarization-induced affinity modulation of muscarinic receptors [5,8,9,12,14,22]. One of these results showed that the L2 and L3 loops in the muscarinic receptor are implicated both in the receptor coupling to trimetric G-proteins and in the depolarization-induced modulation of its affinity for agonists [8,9]. These findings are in accordance with the indispensable role of the depolarization-induced activation of trimetric Go-proteins in the depolarization-induced modulation of the muscarinic receptors' affinity for their cholinergic receptor agonists [14].
The pertussis toxin (PTX)-sensitive trimetric Gi-and Go-proteins are activated by muscarinic agonists [1,2,5,12,14]. The activation of these G-proteins was measured in-situ in synaptoneurosomes by the covalent binding of a labeled GTP [14]. The covalent binding of labeled GTP to trimetric Go-proteins was induced by the binding of cholinergic recep- The binding of certain toxins to the open configuration of VDSC either keeps the VDSC in their open configuration (e.g., Batrachotoxin (BTX) and the S-enantiomer of the cardio-tonic drug DPI) or prevents the opening of VDSC (e.g., the R-enantiomer of the cardio-tonic drug DPI) [13,[17][18][19][20][21].
Muscarinic cholinergic receptor agonists dose-dependently enhanced the specific binding of [ 3 H]BTX to open VDSC in the synaptoneurosomes at resting membrane potential, while Na + entry was blocked (Refs. [13,15]; Figure 1). Thus, these findings suggest that binding of cholinergic receptor agonists to muscarinic receptors induces the opening of VDSC at resting membrane potential and that the 22 Na + entry is blocked by TTX, which blocks sodium currents via VDSC in the brain (Ref. [15]; Figure 1).
A reciprocal effect of the VDSC gating on the affinity of muscarinic receptors for their agonists was identified as well [12,13]. The high affinity binding of [ 3 H]acetylcholine to Many findings identified the involvement of muscarinic receptor coupled trimetric G-proteins in the depolarization-induced affinity modulation of muscarinic receptors [5,8,9,12,14,22]. One of these results showed that the L2 and L3 loops in the muscarinic receptor are implicated both in the receptor coupling to trimetric G-proteins and in the depolarization-induced modulation of its affinity for agonists [8,9]. These findings are in accordance with the indispensable role of the depolarization-induced activation of trimetric Go-proteins in the depolarization-induced modulation of the muscarinic receptors' affinity for their cholinergic receptor agonists [14].
The pertussis toxin (PTX)-sensitive trimetric Gi-and Go-proteins are activated by muscarinic agonists [1,2,5,12,14]. The activation of these G-proteins was measured in-situ in synaptoneurosomes by the covalent binding of a labeled GTP [14]. The covalent binding of labeled GTP to trimetric Go-proteins was induced by the binding of cholinergic recep- Many findings identified the involvement of muscarinic receptor coupled trimetric G-proteins in the depolarization-induced affinity modulation of muscarinic receptors [5,8,9,12,14,22]. One of these results showed that the L2 and L3 loops in the muscarinic receptor are implicated both in the receptor coupling to trimetric G-proteins and in the depolarization-induced modulation of its affinity for agonists [8,9]. These findings are in accordance with the indispensable role of the depolarization-induced activation of trimetric Go-proteins in the depolarization-induced modulation of the muscarinic receptors' affinity for their cholinergic receptor agonists [14].
The pertussis toxin (PTX)-sensitive trimetric Gi-and Go-proteins are activated by muscarinic agonists [1,2,5,12,14]. The activation of these G-proteins was measured in-situ in synaptoneurosomes by the covalent binding of a labeled GTP [14]. The covalent binding of labeled GTP to trimetric Go-proteins was induced by the binding of cholinergic receptor agonists to muscarinic receptors as well as in response to membrane depolarization [14].
Go-proteins are a highly conserved subtype of trimetric G-proteins, expressed in excitable tissues of numerous species [23]. Go-proteins are highly expressed in several regions of the mammalian brain and in peripheral nervous tissues. In addition, Go-proteins are highly expressed in the cardiac atria [23]. Unlike Gi-proteins, Go-proteins are activated in response to membrane depolarization, even in the absence of receptor stimulation [14]. Activation of Go-proteins was measured in-situ by tracing the covalent binding of [α 32 P]GTPazidoanilide under UV-irradiation in depolarized synaptoneurosomes [14]. No covalent binding of [α 32 P]GTP-azidoanilide to Go proteins was measured at resting membrane potential [14]. Furthermore, [α 32 P]GTP-azidoanilide binding to Go-proteins in the depolarized synaptoneurosomes was not dependent on depolarization-induced stimulation of muscarinic receptors. Namely, [α 32 P]GTP-azidoanilide binding to Go-proteins in response to membrane depolarization was not abolished in the presence of antagonists of muscarinic cholinergic receptors. Neither was the depolarization-induced [α 32 P]GTP-azidoanilide binding to Go-proteins prevented by antagonists to additional receptors, including adrenergic, dopaminergic, and serotonergic receptors [14]. In contrast, the open configuration of VDSC in the depolarized synaptoneurosomes was indispensable for the depolarizationinduced exchange of GDP by [α 32 P]GTP-azidoanilide in Go-proteins, even when Na + entry was blocked by TTX [14]. In addition, the α-subunit of Go-proteins co-immunoprecipitated with the α-subunit of the VDSC in depolarized synaptoneurosomes [14], indicating a possible interaction between these proteins in the depolarized membranes [14].
Furthermore, the displacement of GDP by GTP in Go-proteins in response to membrane depolarization was accompanied by a depolarization-induced modulation of the high affinity binding of [ 3 H]acetylcholine to muscarinic receptors from a high to a low affinity [14]. In addition, modifications preventing the activation of PTX-sensitive trimetric G-proteins interfered with the depolarization-induced activation of Go-proteins and with the depolarization-induced modulation of muscarinic receptors affinity for their cholinergic agonists [5,[12][13][14]. Thus, PTX-induced ADP-ribosylation of the α-subunit of Go-and Giproteins, as well as the irreversible binding of GDPβS to trimetric G-proteins, prevented the activation of Go-proteins by muscarinic agonists as well as their activation by membrane depolarization [12,14]. Furthermore, these modifications in G-proteins also prevented the depolarization-induced modulation of the high affinity of muscarinic receptors for [ 3 H]acetylcholine into a low affinity [12,14].
These findings associate the depolarization-induced exchange of GDP by [α 32 P]GTPazidoanilide in activated Go-proteins with the depolarization-induced affinity modulation of muscarinic receptors for [ 3 H]acetylcholine [12,14].
Furthermore, the open configuration of VDSC was a pre-requisite for the effect of G-protein activation on muscarinic affinity modulation (even when Na + entry was blocked) [12][13][14]. Thus, the effect of a permanent binding of GTP to G-proteins (Gpp(NH)p) keeping G-proteins activated on the affinity modulation of muscarinic receptors for [ 3 H]acetylcholine was eliminated when the opening of VDSC was prevented [14]. A permanent binding of Gpp(NH)p reduced the high affinity of the muscarinic receptor for [ 3 H]acetylcholine, but only when the VDSC were kept in their open configuration in synaptoneurosomes treated with BTX or the S-enantiomer of DPI (Refs. [12][13][14]; Figure 3). Preventing the open configuration of the VDSC by the binding of the R-enantiomer of DPI to the VDSC [20] prevented the effect of persistent activation of G-proteins on the high-to low-affinity modulation of muscarinic receptors for [ 3 H]acetylcholine (Ref. [14]; Figure 3). According to these results, the open configuration of the VDSC was required for affinity modulation of muscarinic receptors by activation of G-proteins (Figure 3).
choline, but only when the VDSC were kept in their open configuration in synaptoneurosomes treated with BTX or the S-enantiomer of DPI (Refs. [12][13][14]; Figure 3). Preventing the open configuration of the VDSC by the binding of the R-enantiomer of DPI to the VDSC [20] prevented the effect of persistent activation of G-proteins on the high-to lowaffinity modulation of muscarinic receptors for [ 3 H]acetylcholine (Ref. [14]; Figure 3). According to these results, the open configuration of the VDSC was required for affinity modulation of muscarinic receptors by activation of G-proteins (Figure 3).  The binding of certain toxins to the open configuration of VDSC either keeps the VDSC in their open configuration (e.g., Batrachotoxin (BTX) and the S-enantiomer of the cardio-tonic drug DPI) or prevents the opening of VDSC (e.g., the R-enantiomer of the cardio-tonic drug DPI) [13,[17][18][19][20][21].
Muscarinic cholinergic receptor agonists dose-dependently enhanced the specific binding of [ 3 H]BTX to open VDSC in the synaptoneurosomes at resting membrane potential, while Na + entry was blocked (Refs. [13,15]; Figure 1). Thus, these findings suggest that binding of cholinergic receptor agonists to muscarinic receptors induces the opening of VDSC at resting membrane potential and that the 22 Na + entry is blocked by TTX, which blocks sodium currents via VDSC in the brain (Ref. [15]; Figure 1).
A reciprocal effect of the VDSC gating on the affinity of muscarinic receptors for their agonists was identified as well [12,13]. The high affinity binding of [ 3 H]acetylcholine to muscarinic receptors in the brain synaptoneurosomes was substantially reduced under membrane depolarization [12,13], and the open configuration of VDSC was indispensable for this voltage-induced affinity change in the muscarinic receptors, even when Na + entry was blocked [12][13][14]. Thus, a depolarization-induced reduction in the high-affinity binding of [ 3 H]acetylcholine to muscarinic receptors was not measured when the opening of VDSC was prevented by the binding of the R-enantiomer of the cardiotonic drug DPI to the VDSC [12][13][14]20] (Figure 2). According to these results, the open configuration of the VDSC (but not the Na + current) was indispensable for the depolarization-induced modulation of the muscarinic receptors' affinity for cholinergic agonists, from a high affinity to a low affinity (Refs. [12][13][14]; Figure 2). The binding of antagonists to muscarinic receptors was not similarly affected by membrane depolarization or by the open configuration of the VDSC [5,12,13].
) and in the absence of Gpp(NH)p (  Figure 1). Thus, these findings suggest binding of cholinergic receptor agonists to muscarinic receptors induces the opening DSC at resting membrane potential and that the 22 Na + entry is blocked by TTX, which ks sodium currents via VDSC in the brain (Ref. [15]; Figure 1). A reciprocal effect of the VDSC gating on the affinity of muscarinic receptors for their nists was identified as well [12,13]. The high affinity binding of [ 3 H]acetylcholine to scarinic receptors in the brain synaptoneurosomes was substantially reduced under brane depolarization [12,13], and the open configuration of VDSC was indispensable this voltage-induced affinity change in the muscarinic receptors, even when Na + entry blocked [12][13][14]. Thus, a depolarization-induced reduction in the high-affinity bindof [ 3 H]acetylcholine to muscarinic receptors was not measured when the opening of SC was prevented by the binding of the R-enantiomer of the cardiotonic drug DPI to VDSC [12][13][14]20] (Figure 2). According to these results, the open configuration of the SC (but not the Na + current) was indispensable for the depolarization-induced modun of the muscarinic receptors' affinity for cholinergic agonists, from a high affinity to w affinity (Refs. [12][13][14]; Figure 2). The binding of antagonists to muscarinic receptors not similarly affected by membrane depolarization or by the open configuration of VDSC [5,12,13].  [13,[17][18][19][20][21].
Muscarinic cholinergic receptor agonists d binding of [ 3 H]BTX to open VDSC in the synapto tial, while Na + entry was blocked (Refs. [13,15] that binding of cholinergic receptor agonists to m of VDSC at resting membrane potential and that blocks sodium currents via VDSC in the brain (R A reciprocal effect of the VDSC gating on th agonists was identified as well [12,13]. The hig muscarinic receptors in the brain synaptoneuro membrane depolarization [12,13], and the open c for this voltage-induced affinity change in the m was blocked [12][13][14]. Thus, a depolarization-ind ing of [ 3 H]acetylcholine to muscarinic receptors VDSC was prevented by the binding of the R-en the VDSC [12][13][14]20] (Figure 2). According to th VDSC (but not the Na + current) was indispensab lation of the muscarinic receptors' affinity for ch a low affinity (Refs. [12][13][14]; Figure 2). The bindi was not similarly affected by membrane depola the VDSC [5,12,13]. , choline, but only when the VDSC were kept in their open configuration in synaptoneurosomes treated with BTX or the S-enantiomer of DPI (Refs. [12][13][14]; Figure 3). Preventing the open configuration of the VDSC by the binding of the R-enantiomer of DPI to the VDSC [20] prevented the effect of persistent activation of G-proteins on the high-to lowaffinity modulation of muscarinic receptors for [ 3 H]acetylcholine (Ref. [14]; Figure 3). According to these results, the open configuration of the VDSC was required for affinity modulation of muscarinic receptors by activation of G-proteins (Figure 3). s to the open configuration of VDSC either keeps the n (e.g., Batrachotoxin (BTX) and the S-enantiomer of the ts the opening of VDSC (e.g., the R-enantiomer of the . ptor agonists dose-dependently enhanced the specific in the synaptoneurosomes at resting membrane potend (Refs. [13,15]; Figure 1). Thus, these findings suggest or agonists to muscarinic receptors induces the opening tential and that the 22 Na + entry is blocked by TTX, which in the brain (Ref. [15]; Figure 1). SC gating on the affinity of muscarinic receptors for their 12,13]. The high affinity binding of [ 3 H]acetylcholine to synaptoneurosomes was substantially reduced under , and the open configuration of VDSC was indispensable hange in the muscarinic receptors, even when Na + entry olarization-induced reduction in the high-affinity bindrinic receptors was not measured when the opening of ing of the R-enantiomer of the cardiotonic drug DPI to ccording to these results, the open configuration of the as indispensable for the depolarization-induced modu-' affinity for cholinergic agonists, from a high affinity to re 2). The binding of antagonists to muscarinic receptors mbrane depolarization or by the open configuration of d the involvement of muscarinic receptor coupled trimetric ation-induced affinity modulation of muscarinic receptors results showed that the L2 and L3 loops in the muscarinic in the receptor coupling to trimetric G-proteins and in the ulation of its affinity for agonists [8,9]. These findings are in sable role of the depolarization-induced activation of trimetrization-induced modulation of the muscarinic receptors' afeptor agonists [14]. X)-sensitive trimetric Gi-and Go-proteins are activated by ,14]. The activation of these G-proteins was measured in-situ covalent binding of a labeled GTP [14]. The covalent binding o-proteins was induced by the binding of cholinergic recepceptors as well as in response to membrane depolarization conserved subtype of trimetric G-proteins, expressed in exspecies [23]. Go-proteins are highly expressed in several ren and in peripheral nervous tissues. In addition, Go-proteins rdiac atria [23]. Unlike Gi-proteins, Go-proteins are activated olarization, even in the absence of receptor stimulation [14]. as measured in-situ by tracing the covalent binding of r UV-irradiation in depolarized synaptoneurosomes [14]. No P-azidoanilide to Go proteins was measured at resting memmore, [α 32 P]GTP-azidoanilide binding to Go-proteins in the mes was not dependent on depolarization-induced stimula-Namely, [α 32 P]GTP-azidoanilide binding to Go-proteins in arization was not abolished in the presence of antagonists of tors. Neither was the depolarization-induced [α 32 P]GTP-azteins prevented by antagonists to additional receptors, inergic, and serotonergic receptors [14]. In contrast, the open depolarized synaptoneurosomes was indispensable for the ange of GDP by [α 32 P]GTP-azidoanilide in Go-proteins, even ). The non-specific binding of [ 3 H]ACh was measured in the presence of 1 µM atropine. (Ref. [12]).
Thus, since the affinity of muscarinic receptors for agonists is modulated by activation of PTX-sensitive Gi-and Go-proteins [14], and Go-proteins are activated by membrane depolarization, membrane-depolarization-induced activation of Go-proteins could modulate the affinity of muscarinic receptors for agonists (Figures 2 and 3).
Furthermore, the depolarization-induced opening of VDSC was indispensable for the depolarization-induced activation of Go-protein as well as for the depolarization-induced affinity modulation of muscarinic receptors (Refs. [12][13][14]; Figures 2 and 3).
Thus, according to these results, VDSC gating [17][18][19][20][21], acting as the voltage sensor generating activation of Go-proteins in response to membrane depolarization, causes the affinity modulation of the muscarinic receptor for cholinergic agonists in response to membrane depolarization.
This mechanism is in accordance with the suggested role of loops L2 and L3 in the muscarinic receptor, which are implicated in the coupling of the muscarinic receptor with G-proteins and in the depolarization-induced affinity modulation of the muscarinic receptors [8,9,22]. Moreover, the indispensable role of the open configuration of VDSC in the depolarization-induced affinity modulation of muscarinic receptors for their cholinergic agonists [12][13][14] may explain the discrepancy between the measured gating current even when charges in loop L3 were missing and the attributed gating current to charge movement in the L3 loop of muscarinic receptors [9].
So, in addition to other identified inconsistencies [9,11], an embedded charge movement in the muscarinic receptor [6,8] does not seem to be in line with findings indicating that the depolarization-induced modifications in VDSC are indispensable for depolarizationinduced modulation of the muscarinic receptor affinity for their cholinergic agonists (Refs. [12][13][14]; Figures 2 and 3).
Notably, modifications in G-proteins preventing their activation did not interfere with muscarinic cholinergic agonists inducing the opening of VDSC at resting membrane potential [15]. This result may exclude the involvement of G-proteins' activation in the opening of VDSC by cholinergic agonists that bind with a high affinity to muscarinic receptors at resting membrane potential [12][13][14]. Thus, under physiological conditions, muscarinic agonists inducing the opening of VDSC at resting potential could promote repetitive firing, as identified in pacemakers' activity [24,25]. Depolarization-induced modulation of the muscarinic receptors' affinity for agonists from high to low affinity (Refs. [5,[12][13][14], Figure 2) has been associated with signal transduction mechanisms mediated by activation of G-proteins, phospholipase-C (PLC) activation, inositol 1,4,5-trisphosphate (IP 3 )-induced Ca 2+ -release from intracellular stores [1], modulation of ion currents [1,2], and activation of phosphorylation cascades [1].
In summary, a mechanism suggesting that the affinity of muscarinic receptors for their cholinergic agonists is modulated by membrane depolarization due to a voltage-induced charge movement in the muscarinic receptor is inconsistent with findings indicating the indispensable role of the VDSC gating in the depolarization-induced modulation of the muscarinic receptor's affinity for cholinergic agonists [12][13][14].