Genome-Wide Identification and Phylogenetic and Expression Analyses of the PLATZ Gene Family in Medicago sativa L.

The PLATZ family is a novel class of plant-specific zinc finger transcription factors with important roles in plant growth and development and abiotic stress responses. PLATZ members have been identified in many plants, including Oryza sativa, Zea mays, Triticum aestivum, Fagopyrum tataricum, and Arabidopsis thaliana; however, due to the complexity of the alfalfa reference genome, the members of the PLATZ gene family in alfalfa (Medicago sativa L.) have not been systematically identified and analyzed. In this study, 55 Medicago sativa PLATZ genes (MsPLATZs) were identified in the alfalfa “Xinjiangdaye” reference genome. Basic bioinformatic analysis was performed, including the characterization of sequence lengths, protein molecular weights, genomic positions, and conserved motifs. Expression analysis reveals that 7 MsPLATZs are tissue-specifically expressed, and 10 MsPLATZs are expressed in all examined tissues. The transcriptomic expression of these genes is obvious, indicating that these MsPLATZs have different functions in the growth and development of alfalfa. Based on transcriptome data analysis and real-time quantitative PCR (RT-qPCR), we identified 22, 22, and 21 MsPLATZ genes that responded to salt, cold, and drought stress, respectively, with 20 MsPLATZs responding to all three stresses. This study lays a foundation for further exploring the functions of MsPLATZs, and provides ideas for the improvement of alfalfa varieties and germplasm innovation.


Introduction
Transcription factor is a protein that binds to specific DNA sequences, often containing one or more DNA-binding domains, and regulates the expression of target genes by identifying cis DNA sequences in the promoters of target genes [1,2]. Transcription factors play an important role in improving stress resistance in plants. Therefore, it is important to study the biological functions and regulatory mechanisms of important transcription factors under abiotic stress conditions. The typical DNA-binding regions in plant transcription factors include the AP2/EREBP domain [3], MYB domain [4], WRKY domain [5], zinc finger domain [6], and bZIP domain [7]. The PLATZ family is a novel class of plant-specific zinc finger transcription factors initially isolated from peas (first member named plant AT-rich sequence and zinc-binding protein 1, PLATZ 1), whose members contain two highly conserved protein zinc finger motifs (C-x 2 -H-x 11 -C-x 2 -C-x (4)(5) -C-x 2 -C-x (3)(4)(5)(6)(7) -H-x 2 -H and C-x 2 -C-x (10)(11) -C-x 3 -C) that show nonspecific binding to A/T-rich sequences and repress transcription [8]. PLATZ proteins play important roles in plant growth and development and abiotic stress responses.
Previous studies show that PLATZ proteins play important roles in multiple biological processes in plants, including regulating seed germination, leaf cell proliferation, and Int. J. Mol. Sci. 2023, 24, 2388 2 of 15 senescence. For example, the Oryza sativa PLATZ proteins can regulate the expression of genes involved in rice grain development. The Oryza sativa GL6 (SG6) PLATZ protein knockout mutant gl6/sg6 shows grain length shortening and grain weight reduction, while after GL6/SG6 overexpression, the grains become significantly larger and heavier, and plant height and plant fresh weight are significantly increased [9,10]. The protein encoded by the Floury3 (FL3) gene in Zea mays also belongs to the PLATZ superfamily and is specifically expressed in endosperm cells and can affect endosperm development and nutrient storage [11]. A dominant Arabidopsis thaliana mutant of the PLATZ family transcription factor ORESARA15 shows increases in the leaf life span and leaf size, suggesting that ORE15 could regulate leaf growth [12]. In transcriptome comparison studies of normal villous (FL) and syngenic nonvillous (Fl) diploid cotton fibers, it was found that a PLATZ transcription factor was downregulated after 10 d of flowering in Fl compared with FL, suggesting that the PLATZ zinc finger transcription factor may play a role in the cotton fiber initiation and elongation stage [13]. A PLATZ protein plays an important role in the transition from primary growth to secondary growth during Populus stem development [14]. PLATZ gene expression is very different in mature and immature sugarcane tissues of sugarcane high-fiber genotypes, and it is speculated that PLATZ may be a transcriptional regulator of secondary cell wall synthesis [15].
Many studies show that PLATZ transcription factors are not only widely involved in plant growth and development, but can also respond to multiple abiotic stresses. At-PLATZ1 plays an important role in seed dehydration tolerance. AtPLATZ1 overexpression in wild-type Arabidopsis thaliana results in partial seed dehydration tolerance in vegetative tissues, contributing to its ability to improve low water utilization and survive in water-deficient environments, and AtPLATZ1 regulates drought resistance in vegetative tissues [16]. The expression of Zea mays PLATZ1 homologs is strongly induced by drought stress [14][15][16], indicating that PLATZ proteins are key factors regulating drought stress in Zea mays [17]. The heterologous expression of GhPLATZ1 in Arabidopsis thaliana can inhibit ABA biosynthesis during seed development and regulate the Arabidopsis thaliana response to salt and osmotic stress during seed germination and seedling establishment. Therefore, it is speculated that GhPLATZ1 may regulate osmotic stress and salt stress responses during cotton seed germination and seedling establishment [18].
Alfalfa (Medicago sativa L.) is a perennial legume plant widely planted worldwide as an economic forage plant [19]. It has the advantages of high adaptability, high protein content, and rich nutritional value [20,21]. Alfalfa has a long history of cultivation in China. With the continuous development of animal husbandry in China in recent years, the demand for alfalfa has also greatly increased, and research on alfalfa is intensifying [22]. Mining the excellent genes of Medicago sativa is of great significance to improve researching alfalfa at the theoretical level and to better guide the production of alfalfa. Therefore, the identification of PLATZ proteins in Medicago sativa is important based on the identified regulatory roles of PLATZ proteins in plant seed growth and development and the functional potential associated with plant stress resistance [9,12].
In this study, 55 PLATZ proteins were identified in the alfalfa genome. The chromosomal localizations, gene structures, conserved motifs, and cis-acting elements in the promoter regions of 55 MsPLATZs were comprehensively studied, and the evolutionary relationships of Medicago sativa PLATZs with those of three representative species, Arabidopsis thaliana, Medicago truncatula, and Glycine max, were analyzed. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to determine the different tissue expression profiles of MsPLATZs in alfalfa. In addition, the expression profiles of MsPLATZs under three abiotic stresses (salt, drought, and cold stress) were also studied to determine the roles of MsPLATZs in different biological processes in alfalfa. These results provide valuable information for the identification of candidate MsPLATZ genes involved in various abiotic stress responses in alfalfa.

Identification of PLATZ Genes in the Alfalfa Genome
A total of 55 PLATZ genes were identified in the genome of Chinese landraces (Medicago sativa L. XinJiangDaYe) on the basis of HMM and domain analysis [23]. Basic information about the members of the alfalfa PLATZ gene family is listed in Tables 1 and S1. As shown in Table 1, the full-length coding sequences (CDS), predicted protein products, molecular weights (Mw), and isoelectric points (pI) of the MsPLATZ genes are quite different, ranging from 156 (

Phylogenetic Analysis and Classification of MsPLATZ Proteins
To elucidate the evolutionary relationship between the MsPLATZ proteins and the model plant AtPLATZ proteins, a neighbor-joining tree containing 55 identified MsPLATZs and 12 AtPLATZs was constructed with MEGA software (Figure 2). The 67 PLATZ proteins were divided into five groups (I-V), and the MsPLATZ proteins were distributed in all five groups (I-V). Among the five groups, group V contains the most PLATZ proteins, with 21 members, including 15 MsPLATZs and 6 AtPLATZs, and group IV has the fewest PLATZ members, including 4 MsPLAYTZs and 1 AtPLATZ. Group II contains 3 AtPLATZs and 15 MsPLATZs, and the number of MsPLATZs is the same as that in group V. Group III contains only 10 MsPLATZ members. Group I has a total of 13 PLATZ members, including 11 MsPLATZs and 2 AtPLATZs. In addition, the phylogenetic tree of MsPLATZs was constructed based on the grouping in the phylogenetic tree and analyzed for gene structure and motif components among the groups ( Figure 3A).

Figure 2.
Phylogenetic tree of the PLATZ genes in Medicago sativa and Arabidopsis thaliana. I-V: PLATZ genes were divided into five groups and were represented using different colors; Neighbor-joining (NJ) was used to generate the unrooted phylogenetic tree with the maximum likelihood method (1000 bootstrap replicates).

Gene structure and Conserved Motif Analysis of MsPLATZ Genes
To understand the structural composition of the MsPLATZ genes, we investigated the exon and intron structure of the MsPLATZ genes based on the Medicago sativa genomic DNA sequence ( Figure 3B). The results show that the gene structure of the MsPLATZ genes presents some differences in the five groups, but also exhibits some similarity between the groups. The vast majority of genes (51 of 55, 92.73%) have five exons or fewer, with the greatest percentage containing four exons (40 of 55, 72.73%); only four genes, MsPLATZ50/10/17/34, have more than 5 exons, and MsPLATZ34 has 13 exons. Among the genes in group I, MsPLATZ1 has only one exon, and all other genes contain four exons. In group III, similar to group I, only MsPLATZ10 has seven exons, and all other genes contain four exons. Group IV includes only four members, all of which contain four exons. Group V induces the gene with the largest number of exons, MsPLATZ34, with 13 exons. The number of exons varies among the members of group II, ranging from 1-8, but most of these genes contain four exons. To identify the conserved motifs of the MsPLATZs, we performed an assay analysis with the MEME website. A total of 10 conserved motifs were detected in the MsPLATZ genes, which were designated Motifs 1-10 ( Figure 3C). Motif 3, motif 7, motif 1, motif 2, and motif 6 represent 100%, 98.18%, 94.55%, 81.82%, and 80%, respectively, of the five most abundant pairs of the 10 motifs, indicating that these motifs are the most important components of MsPLATZ proteins. Motif 7 is not present in MsPLATZ19, indicating possible sequence loss in the course of evolution. The members within each group present similar gene structures and motifs, with some motifs only being present in specific groups, such as motif 10, which is only present in group IV, and motif 9, which is only present in group V. Thus, the MsPLATZ genes of the same group have similar gene structures and motif compositions, while MsPLATZ genes from different groups may have specific structures, indicating that the conservation and diversity of MsPLATZ function have evolved during long-term evolution.

Gene Duplication Events and Synteny Analysis of MsPLATZ Genes
To further investigate possible gene duplication events among the MsPLATZ genes, we mapped the predicted MsPLATZs using the genome database of Medicago sativa (XinJiang DaYe) [23]. The results show possible tandem duplication events among genes such as MsPLATZ18/19/20 on chromosome chr2.4 (Figure 1). The analysis of segmental duplication events in the MsPLATZ family reveal a total of 94 pairs of fragment duplications, as found in MsPLATZ21 and MsPLATZ1, located on different chromosomes (Figure 4). These results suggest that duplication events have been widely involved in the evolution of MsPLATZs.  Figure 5). The collinearity identified between Medicago sativa, Medicago truncatula, and Glycine max is significantly higher than that between Medicago sativa and Arabidopsis thaliana, indicating that the distribution of PLATZ genes is relatively conserved among the three legume species.

Expression Patterns of MsPLATZ Genes in Different Tissues of Medicago sativa
Genome-wide gene expression data for some tissues of alfalfa, such as roots, elongated stems, pre-elongated stems, leaves, flowers, and nodules, are publicly available. As shown in Figure 6A, only 23 of the 55 MsPLATZ genes are expressed in the six tissues studied, and other unexpressed genes may be expressed in other specific tissues or

Expression Patterns of MsPLATZ Genes in Different Tissues of Medicago sativa
Genome-wide gene expression data for some tissues of alfalfa, such as roots, elongated stems, pre-elongated stems, leaves, flowers, and nodules, are publicly available. As shown in Figure 6A, only 23 of the 55 MsPLATZ genes are expressed in the six tissues studied, and other unexpressed genes may be expressed in other specific tissues or specifically expressed under certain biotic or abiotic stresses. The transcript abundance of the 23 expressed MsPLATZ genes also varies in the six tissues, indicating that the functions of each gene are also significantly different. A total of 7 out of 23 MsPLATZ genes are detected in only one tissue, indicating tissue expression specificity. The seven genes are MsPLATZ47, expressed in roots; MsPLATZ31, expressed in pre-elongated stems; and MsPLATZ26/43/38/39/40, expressed in flowers. The only gene expressed in three tissues is MsPLATZ6, which is expressed in flowers, leaves, and nodules. Two genes, MsPLATZ1 and MsPLATZ54, are expressed in two tissues: MsPLATZ1 is expressed in flowers and elongated stems, and MsPLATZ54 is expressed in nodules and roots. MsPLATZ32 is expressed in five of the tissues with the exception of leaves, and MsPLATZ33/51 is expressed five of the tissues with the exception of pre-elongated stems. Ten genes are expressed in six tissues, including MsPLATZ41/37/36, and although they are expressed in six tissues, their expression is significantly higher in flowers than in other tissues. These results indicate significant functional differences between MsPLATZ genes, and suggest that they play important roles in regulating the growth and development of M. sativa.

Analysis of Promoter Cis-Acting Elements of MsPLATZ Genes
To further explore the functional potential of the MsPLATZ genes, we analyzed the cisacting elements withing the 2 kb upstream region of the MsPLATZ start codon (ATG) using the PlantCARE database (http://bioinformatics.psb.ugent.be/webtools/plantcare/html/, accessed on 16 August 2022). For example (Table S2), various cis-acting elements were identified. Photoreaction elements such as Box 4 and the GT1 motif are widely distributed in the promoter regions of MsPLATZ genes. In addition to photo-responsive elements, hormone response elements such as ABREs, cell-cycle-related elements such as circadian elements, stress-related elements such as MBSs, and growth and development elements such as CAT boxes are found. Notably, the number of hormone response elements varies greatly among the numerous MsPLATZ genes compared to the other elements. As shown in Figure 7, MsPLATZ42 contains only two auxin-related response elements. In addition to the two auxin-related response elements, MsPLATZ27 has four elements related to the abscisic acid response, four elements related to the methyl jasmonate response, and one element related to the gibberellin response. These results suggest that different MsPLATZ genes may function under different hormonal stimuli.

Differential Expression of MsPLATZ Genes under Different Abiotic Stress Treatments
To understand the responses of the 55 MsPLATZ genes involved in abiotic stress responses, we further analyzed the MsPLATZ genes that were differentially expressed under three abiotic stresses (salt, cold, and drought). As shown in Figure 8A Table S3. The results show that MsPLATZ49/51/54 expression significantly increases with prolonged salt and drought stress treatment times ( Figure 8D-I). This further indicates that the MsPLATZ49/51/54 genes may play an important role in stress resistance. Finally, it is concluded that the results obtained for the different genes under the different stress treatments differ significantly, which also indicates that the genes have different functions and specificities. All candidate genes selected in this study are listed in Table S4.

Discussion
PLATZ transcription factors are a class of plant-specific zinc-dependent DNA-binding proteins that have been shown by multiple studies to be widely involved in plant growth and development and to play an important role in the response to abiotic stresses [24]. The identification of PLATZ gene family members at the genome-wide level has been confirmed in many plants. For example, 12 PLATZ genes were identified in Arabidopsis thaliana [25], 15 PLATZ genes in Oryza sativa [25], 17 PLATZ genes in Zea mays [25], 14 PLATZ genes in Fagopyrum tataricum [26], 11 PLATZ genes in Ginkgo biloba [27], and 62 PLATZ genes in Triticum aestivum [28]. However, systematic analyses and in-depth studies at the genome-wide level are still lacking for Medicago sativa PLATZ gene families. In this study, we identified a total of 55 PLATZ genes in the alfalfa reference genome, all of which contain a conserved PLATZ domain. Sequence analysis, basic bioinformatics analysis, evolutionary analysis, and expression analysis were also performed. The results indicate that the MsPLATZ genes play an important role in the growth and development of M. sativa and may cause its resistance to abiotic stresses such as cold, drought, and salinity.
Phylogenetic tree analysis was used to help predict gene function. PLATZ transcription factors regulate root growth in Arabidopsis thaliana. For example, in Arabidopsis thaliana, RITF1 (PLATZ7), which acts as a receptor of the RGF1 small peptide, is involved in regulating ROS signaling and then in regulating the development of the root meristem [29]. In this study, we find that MsPLATZ52/47/50/48/46/22/30/26/17/14/55/45/43/44/42 cluster into the same group (group II) as AtRGFI (At2G12646, Figure 2). Among these MsPLATZ genes, only MsPLATZ47 is specifically expressed in alfalfa roots ( Figure 5). Therefore, through the phylogenetic analysis and expression analysis of the MsPLATZ genes, we infer that MsPLATZ47 may also play an important role in alfalfa root development.
Plants can respond to and coordinate growth and stress resistance by regulating hormone production, distribution, and signaling to promote survival under abiotic and biotic stresses [30]. Zinc finger transcription factors are a relatively large family of plant transcription factors (approximately 15% of the total family) that are involved in regulating the expression of multiple genes in response to cold, salt, drought, osmotic stress, and oxidative stress [31,32]. AtPLATZ1 and AtPLATZ12 were identified as major genes positively regulating access to dehydration tolerance in Arabidopsis thaliana seeds and vegetative tissues [13]. Transgenic plants overexpressing AtPLATZ2 show higher sensitivity to salt stress, which inhibits CBL4/SOS3 promoter activity by directly binding to A/T-rich sequences in the CBL4/SOS3 and CBL10/SCaBP8 promoters in vitro and in vivo [24]. In this study, we find 22, 21, and 22 MsPLATZ genes responding to cold, drought, and salt stress, respectively ( Figure 8A-C). Phylogenetic analysis shows that both AtPLATZ2 (AT1G76590) and 15 MsPLATZ members (MsPLATZ34/23/31/27/35/38/36/40/37/41/39/54/51/49/53) belong to group V (Figure 2). Under salt stress, the 11 MsPLATZ members are differentially expressed, among which the expression of MsPLATZ49/51/54 is significantly upregulated (Figure 8D-I). Therefore, these genes may be important candidates for the salt stress response in alfalfa.
Alfalfa is one of the most important feed crops in the world and is known as the "king of forage grass" [20]. It shows high adaptability, high yields, and a nutrient-rich composition and is considered a high-quality diet for herbivores [21]. As alfalfa is often subjected to abiotic stresses such as cold, drought, and salinity during growth, its quality and production decrease [33,34]. Therefore, it is important to identify good alfalfa stress resistance genes to guide alfalfa production. PLATZ transcription factors are zinc finger transcription factors, which constitute a relatively large family of plant transcription factors that are widely involved in plant growth and development and play an important role in the response to abiotic stresses [24]. The results of this study provide useful information for the MsPLATZ gene family, and the functions of MsPLATZs in response to various abiotic stresses need to be confirmed by more experiments in the future.

Identification of MsPLATZ Genes in the Medicago sativa Genome
The alfalfa genome comes from the Alfalfa Genome Project (https://fgshare.com/ projects/whole_genome_sequencing_and_assembly_of_Medicago_sativa/66380, accessed on 5 August 2022). Arabidopsis thaliana protein sequences and Medicago truncatula genome were obtained from the Arabidopsis thaliana Information Resource (TAIR) (https://www. arabidopsis.org/, accessed on 9 August 2022), and (http://www.medicagogenome.org/, accessed on 9 August 2022). The largest number of PLATZ genes was screened from the alfalfa genome by BLASTp methods, and the hidden MarKov model (HMM) profiles corresponding to the PLATZ domain (PF04640) were downloaded from the Pfam protein family database (https://pfam.xfam.org/, accessed on 13 August 2022). In total, 55 MsPLATZ genes were identified in the alfalfa genome using BLAST with a cutoff Evalue > 1e −10 . All putative MsPLATZ genes integrating the results of the HMM retrieval and BLASTP operations were submitted to the NCBI Conserved Domain Database (CDD, https://www.ncbi.nlm.nih.gov/cdd, accessed on 19 August 2022), SMART (http://smart. embl-heidelberg.de/, accessed on 19 August 2022), and Pfam to examine the existence of the conserved PLATZ domain. The ExPASy website (https://web.expasy.org/compute_pi/, accessed on 8 September 2022) was used to analyze the MsPLATZ gene sequences to obtain the theoretical isoelectric points (pIs) and molecular weights (MWs).

Phylogenetic Analysis and Intron-Exon Structure Determination
The PLATZ protein sequences used to generate the phylogenetic tree were obtained from the UniProt database (https://www.UniProt.org, accessed on 20 August 2022). The PLATZ protein sequences from Medicago sativa and Arabidopsis thaliana were also aligned using the Clustalx2.0 program before the phylogenetic tree was constructed. Phylogenetic trees comparing MsPLATZs and AtPLATZs were constructed with the NJ method, and the specific parameters were the Poisson model and 1000 bootstrap replications in MEGA software (v11.0, Tamura, K., Tokyo, Japan). The classification of MsPLATZs based on the phylogenetic tree was performed according to the method described by Wang et al. [25]. The determination of the conserved motifs in the MsPLATZ proteins was conducted by the MEME online program (http:/meme.nbcr.net/meme/intro.html, accessed on 11 August 2022), and the parameters were set to the optimum mode width of 6 to 200 and the maximum number of motifs of 10.

Gene Duplication, Synteny Analysis and Cis-Element Analysis
MCScanX software [35,36] (Hu, Y., Herndon, USA) was used to analyze the MsPLATZ replication events and detect collinear regions between MsPLATZ and collinear blocks of MsPLATZ genes with Arabidopsis thaliana, Medicago truncatula, and Glycine max. All function and chromosomal location information was obtained by TBtools software [37] (v1.108, Chen, C., GZ, China). The upstream 2 kb sequence was extracted as the promoter region for the prediction of cis-acting elements. The homeopathy components of the promoter sequence were predicted by the online tool PlantCARE [38], and the predicted results were drawn by GSDS online software (v2.0, Hu, B., BJ, China).

Plant Materials and Treatments
The alfalfa seeds (Cultivar Zhongmu No. 1) were preserved in our laboratory at the Institute of Animal Science of the Chinese Academy of Agricultural Sciences. The seedlings were placed in a greenhouse at 24 • C (day)/20 • C (night) with a 16 h light/8 h dark photoperiod in the hydroponic culture medium for two weeks. For cold stress, drought stress and salt stress, refer to He et al. [30]. Three replicates with five single seedlings young leaves in each replicate were collected for each condition. The samples were stored at −80 • C for further RT-PCR experiments.

Transcriptome Data Collection and Analysis
Transcriptomic data from six Medicago sativa tissues (flower, leaf, elongated stem, preelongated stem, nodule, and root) were collected from the NCBI database (SRP055547) [39]. Transcriptomic data for MsPLATZ genes exposed to cold, drought, and salt treatments were collected from the NCBI database (SRR7091780-SRR7091794 and SRR7160313-SRR7160357) [40]. TopHat2 was used to map the obtained clean reads to the reference genome (Xinjiangdaye) [41]. In addition, we applied fragments per kilobase of transcript per million fragments mapped (FPKM) to calculate the gene expression level according to the number of reads mapped to the reference sequence. TBtools software (v1.108, Chen, C., GZ, China) was used for data visualization [37].

Expression Analyses of MsPLATZ Genes by qRT-PCR
Total RNA was extracted from all samples in this study by using TRIzol reagent according to the manufacturer's instructions. Then, the corresponding cDNA was obtained by using the EasyScript First-Strand cDNA Synthesis kit. The RT-PCR primers for these MsPLATZ genes were designed by using Primer 5.0 software. The RT-PCR experiments were conducted by using SYBR Premix Ex Taq (Takara, Maebashi, Japan) on a 7500 Real-Time PCR system (Applied Biosystems, Foster City, CA, USA). Three replicates were designed for each sample, and alfalfa actin gene expression was used for data normalization. The data were quantified by the 2 −∆∆CT method [42].

Conclusions
In this study, the PLATZ gene family members of Medicago sativa were systematically identified and characterized. A total of 55 MsPLATZ proteins were identified that were unevenly distributed on 24 out of the 32 chromosomes in Medicago sativa, and one was not spliced to the chromosomes. Based on the phylogenetic analysis, the MsPLATZ proteins were divided into five groups, each with similar gene structure and motif compositions. In addition, in regard to gene duplications, gene segment duplication rather than gene tandem duplication is the main driving force of MsPLATZ gene evolution. We analyzed the expression levels of 55 MsPLATZ genes in different tissues of Medicago sativa and their responses to three abiotic stresses (salt, cold, and drought). Expression analysis shows that 7 MsPLATZ genes have tissue-specific expression, and 10 MsPLATZ genes are expressed in all tissues. The transcriptomic profiles of these genes vary greatly, indicating that these MsPLATZ genes have different functions in the growth and development of alfalfa. Through transcriptome data analysis and real-time quantitative PCR (RT-qPCR), we identify 22, 21, and 22 MsPLATZ genes that are responsive to cold, drought, and salt stress, respectively. Moreover, the expression of MsPLATZ49/51/54 under salt and drought stress is significant, indicating that MsPLATZ49/51/54 may be related to the regulation of salt and drought stress. The results of this study lay a foundation for the further exploration of the MsPLATZ function and provide ideas for the improvement of alfalfa varieties and the innovation of germplasm resources.