From Fruit Waste to Medical Insight: The Comprehensive Role of Watermelon Rind Extract on Renal Adenocarcinoma Cellular and Transcriptomic Dynamics

Cancer researchers are fascinated by the chemistry of diverse natural products that show exciting potential as anticancer agents. In this study, we aimed to investigate the anticancer properties of watermelon rind extract (WRE) by examining its effects on cell proliferation, apoptosis, senescence, and global gene expression in human renal cell adenocarcinoma cells (HRAC-769-P) in vitro. Our metabolome data analysis of WRE exhibited untargeted phyto-constituents and targeted citrulline (22.29 µg/mg). HRAC-769-P cells were cultured in RPMI-1640 media and treated with 22.4, 44.8, 67.2, 88.6, 112, 134.4, and 156.8 mg·mL−1 for 24, 48, and 72 h. At 24 h after treatment, (88.6 mg·mL−1 of WRE) cell proliferation significantly reduced, more than 34% compared with the control. Cell viability decreased 48 and 72 h after treatment to 45% and 37%, respectively. We also examined poly caspase, SA-beta-galactosidase (SA-beta-gal), and wound healing activities using WRE. All treatments induced an early poly caspase response and a significant reduction in cell migration. Further, we analyzed the transcript profile of the cells grown at 44.8 mg·mL−1 of WRE after 6 h using RNA sequencing (RNAseq) analysis. We identified 186 differentially expressed genes (DEGs), including 149 upregulated genes and 37 downregulated genes, in cells treated with WRE compared with the control. The differentially expressed genes were associated with NF-Kappa B signaling and TNF pathways. Crucial apoptosis-related genes such as BMF, NPTX1, NFKBIA, NFKBIE, and NFKBID might induce intrinsic and extrinsic apoptosis. Another possible mechanism is a high quantity of citrulline may lead to induction of apoptosis by the production of increased nitric oxide. Hence, our study suggests the potential anticancer properties of WRE and provides insights into its effects on cellular processes and gene expression in HRAC-769-P cells.


Introduction
Cancer is a pressing global public health concern and ranks notably as the second most common cause of death in the United States [1,2].The year 2023 has presented alarming statistics, as the US National Center for Health Statistics, in collaboration with the Center for Disease Control and Prevention, projected that a staggering 81,800 new cases of kidney cancer have surfaced, culminating in approximately 14,890 deaths [3].An unsettling aspect of cancer treatment lies in the severe adverse effects of numerous anticancer drugs.These effects primarily stem from the drugs' inability to discern between regular and cancerous cells [4].Nevertheless, there is a silver lining.By adopting simple lifestyle modifications such as dietary changes, maintaining optimal body weight, and regular physical exercise, a significant portion of cancers (ranging from 30% to 40%) can be potentially thwarted [5].An encouraging revelation is that an increased consumption of vegetables and fruits might hold the key to averting approximately 20% of annual cancer-related mortalities.The rationale lies in their intrinsic safety, minimal toxicity, potent antioxidant properties, and widespread acceptance as dietary supplements.Thus, in the current era of cancer research, the potential benefits of fruits and vegetables have become a focal point of scientific inquiry [1].This has spurred medical professionals worldwide to intensively explore novel anticancer compounds sourced naturally and to delve into complementary herbal therapies [6,7].
One such fruit garnering attention is the watermelon.Hailing from the Cucurbitaceae family, watermelon is a prominent horticultural crop cherished for its succulent fruits [8].While it is a natural diuretic and finds its way into various culinary delights, its rind (WR) is often discarded.Extracting bioactive compounds from this waste could revolutionize the agricultural food chain and present sustainable solutions [7].Previous research has illuminated the myriad of benefits WR holds, attributing its antioxidant, free radical scavenging, and anti-microbial capabilities to the array of phenolic compounds it houses, such as quercetin, myricetin, and more [9][10][11].Intriguingly, citrulline, a non-essential amino acid often likened to Viagra, is found in greater abundance in WR than in the pulp, further underscoring its potential health benefits [7,12,13].

Objectives
Given the potential benefits and therapeutic properties associated with watermelon, especially its rind, our study seeks to analyze watermelon rind waste using LC-MS to discern and comprehensively document the composition of watermelon rind extract (WRE) to investigate the biological effects of WRE with a specific focus on its implications for cell proliferation, apoptosis, cell migration, and the global transcriptomic profile in HRAC-769-P cells, and to establish a comparative understanding between existing research findings on WRE's inhibitory effects on human cancer cell proliferation and its effects on HRAC-769-P cells.Through our research, we endeavor to bridge existing knowledge gaps, shed light on the implications of WRE on HRAC-769-P cells, and provide a comprehensive understanding of the therapeutic potential of watermelon rind.

Metabolite Analysis of Phytoconstituents of Watermelon Rind Extraction
WR metabolome analysis identified targeted citrulline (22.29 µg/mg) and other untargeted metabolites in our study, including amino acid derivatives, organic acid derivatives, sugar derivatives, and hydroxycinnamic acid derivatives presented in Table 1.Various concentrations of WREs' effects on HRAC-769-P cell proliferation showed dose-dependent cell viability (Figure 1).WRE chemical compounds could affect cell proliferation via L-Citrullin and other chemical constituents.In previous studies, it was observed that the anticancer effects of WRE in vitro were dependent on both the dose and time of exposure [7].The longer duration of exposure was found to increase the potency of WRE and enhance its stability under specific experimental conditions.The percentage of HRAC-769-P cells decreased as the concentration of WRE increased 24, 48, and 72 h after treatment.(Figure 1).At 24 h after treatment, the proportion decreased to 15%, 33%, 40%, 47%, 53%, 59%, and 66%, respectively, compared with controls.Moreover, further cell growth was inhibited significantly at 48 and 72 h post treatment, to 28%, 54%, 69%, 83%, 88%, 93%, and 95% and 39%, 62%, 76%, 91%, 93%, 95%, and 96%, respectively, compared with controls at various concentrations (Figure 1).

HRAC-769-P Cell Proliferation Was Affected by Concentration and Dura
Various concentrations of WREs' effects on HRAC-769-P cell pro dose-dependent cell viability (Figure 1).WRE chemical compounds co liferation via L-Citrullin and other chemical constituents.In previous served that the anticancer effects of WRE in vitro were dependent on time of exposure [7].The longer duration of exposure was found to in of WRE and enhance its stability under specific experimental condition of HRAC-769-P cells decreased as the concentration of WRE increase after treatment.(Figure 1).At 24 h after treatment, the proportion decre 40%, 47%, 53%, 59%, and 66%, respectively, compared with controls.cell growth was inhibited significantly at 48 and 72 h post treatment, 83%, 88%, 93%, and 95% and 39%, 62%, 76%, 91%, 93%, 95%, and 96%, pared with controls at various concentrations (Figure 1).

Apoptosis Versus Senescence
Poly caspase activity was noticed from 0.5 h compared with the 769-P cells (Figure 2).This trend continued up to 2 to 4 h.Compared w concentrations of WRE (44.8 and 88.6 mg) exhibited caspase activity.C (ethanol-0.96%) control, peak poly caspase activity occurred with WRE h, followed by 1 h, respectively.The early induction of caspase activity it began to decrease at 4 h.SA-beta-gal activity with WRE 88.6 mg•mL ited similarity to control(s) activity at 0.5 and 1 h, followed by a declin estingly, the activity was significantly lower in other treatments between

Apoptosis versus Senescence
Poly caspase activity was noticed from 0.5 h compared with the control in HRAC-769-P cells (Figure 2).This trend continued up to 2 to 4 h.Compared with the control, all concentrations of WRE (44.8 and 88.6 mg) exhibited caspase activity.Compared with the (ethanol-0.96%)control, peak poly caspase activity occurred with WRE (88 mg•mL −1 ) at 2 h, followed by 1 h, respectively.The early induction of caspase activity was noticed, and it began to decrease at 4 h.SA-beta-gal activity with WRE 88.6 mg•mL −1 treatment exhibited similarity to control(s) activity at 0.5 and 1 h, followed by a decline (Figure 3).Interestingly, the activity was significantly lower in other treatments between 0.5 and 2 h.Overall, SA-beta-gal activity began to decrease from 2 h in all the treatments.

Assessment of Cell Migration Inhibition and Metastasis Using Wound Healing Assay
The effects of WRE on the progression and migration of HRAC-769-P cells wer uated for 48 h.The wound in the control group was healed 48 h after scratching a layer of cells.When cells were treated with WRE IC50, the wound healing of the scr area was significantly delayed compared with untreated and WRE-treated cells ( 4A).The WRE significantly decreased the wound closure by 93.14% (p < 0.00001) i

Assessment of Cell Migration Inhibition and Metastasis Using Wound Healing Assay
The effects of WRE on the progression and migration of HRAC-769-P cells wer uated for 48 h.The wound in the control group was healed 48 h after scratching a layer of cells.When cells were treated with WRE IC50, the wound healing of the scr area was significantly delayed compared with untreated and WRE-treated cells ( 4A).The WRE significantly decreased the wound closure by 93.14% (p < 0.00001) compared with that of the control group (Figure 4B).An in vitro cell migration a

Assessment of Cell Migration Inhibition and Metastasis Using Wound Healing Assay
The effects of WRE on the progression and migration of HRAC-769-P cells were evaluated for 48 h.The wound in the control group was healed 48 h after scratching a monolayer of cells.When cells were treated with WRE IC50, the wound healing of the scratched area was significantly delayed compared with untreated and WRE-treated cells (Figure 4A).The WRE significantly decreased the wound closure by 93.14% (p < 0.00001) in cells compared with that of the control group (Figure 4B).An in vitro cell migration analysis reveals metastatic potential.Cancer metastasis is the foremost reason for cancer death globally [7,14].In our study, the WRE significantly inhibited cell migration in HRAC-769-P cells.Therefore, WRE components may include potent anticancer agents in decreasing cancer metastasis.

RNAseq Assessment of Differentially Expressed Genes Between WRE-Treated and Control Cells
A total of 26,784,649, 27,470,824, and 25,588,547 raw reads were generated from the control condition of HRAC-769-P cells, whereas 32,424,898, 27,827,568, and 31,744,771 reads were generated with WRE treatment (44.8 mg-mL −1 ), respectively.The raw reads were subjected to stringent quality filtering by using a trimmomatic tool, which resulted in 24,702,469, 25,318,621, 23,597,931, 29,939,262, 25,713,431, and 29,270,759 high-quality reads for control and treatment (44.8 mg), respectively.The Q30 percentage of reads in each library was >96%.The reads from the one controlled and two treatment conditions were aligned to the human reference genome (GRCh38.p13)using the STAR universal RNA sequence alignment tool with default parameters.Then, 96.4,96.3, 96.4,96.5, 96.4, and 96.3 percent quality-filtered reads were mapped to the reference genomes for WRC and WRT44, respectively; ~3.6% of the reads remained unmapped (Table 2).The volcano plot in (Figure 5) shows each treatment's total up-and downregulated genes based on −log10 (p-value) and log2 fold change.It includes 149 upregulated and 37 downregulated genes.The top 10 up-and downregulated DEGs shared among the two treatments are presented in Supplementary Table S1.

RNAseq Assessment of Differentially Expressed Genes between WRE-Treated and Control Cells
A total of 26,784,649, 27,470,824, and 25,588,547 raw reads were generated from the control condition of HRAC-769-P cells, whereas 32,424,898, 27,827,568, and 31,744,771 reads were generated with WRE treatment (44.8 mg•mL −1 ), respectively.The raw reads were subjected to stringent quality filtering by using a trimmomatic tool, which resulted in 24,702,469,25,318,621,23,597,931,29,939,262,25,713,431, and 29,270,759 high-quality reads for control and treatment (44.8 mg), respectively.The Q30 percentage of reads in each library was >96%.The reads from the one controlled and two treatment conditions were aligned to the human reference genome (GRCh38.p13)using the STAR universal RNA sequence alignment tool with default parameters.Then, 96.4,96.3, 96.4,96.5, 96.4, and 96.3 percent quality-filtered reads were mapped to the reference genomes for WRC and WRT44, respectively; ~3.6% of the reads remained unmapped (Table 2).The volcano plot in (Figure 5) shows each treatment's total up-and downregulated genes based on −log10 (p-value) and log2 fold change.It includes 149 upregulated and 37 downregulated genes.The top 10 up-and downregulated DEGs shared among the two treatments are presented in Supplementary Table S1.In total, 33 (up) and 24 (down) statistically significant differentially expressed genes (DEGs) were identified for WRET44.8mg•mL −1 , which are known to be involved in tumor suppression and anti-cell proliferation (Table 3).In total, 33 (up) and 24 (down) statistically significant differentially expressed genes (DEGs) were identified for WRET44.8mg•mL −1 , which are known to be involved in tumor suppression and anti-cell proliferation (Table 3).

Pathway Enrichment Analysis
A KEGG pathway enrichment analysis of the DEGs from each ecotype showed different pathways enriched across the treatments.DEGs with WRE treatment revealed several enriched pathways such as the NF-Kappa B signaling pathway, the TNF signaling pathway, the neuroactive ligand-receptor interaction, Leukocyte transendothelial migration, the IL-17 signaling pathway, the cytokine-cytokine receptor interaction, the metabolic pathway, and the calcium signaling pathway (Supplementary Figure S1).

Role of Nutraceutical Compounds
Watermelon, notably its rind, is rich in bioactive compounds like lycopene and Lcitrulline, which are reported to exhibit antioxidant, anti-diabetic, and anticancer activities [8].Specifically, WRE has a high concentration of L-citrulline, an amino acid serving as an L-arginine precursor.L-citrulline is an important factor in nitric oxide (NO) synthesis, contributing to various physiological effects, including anticancer activities [7,15].Our study corroborates these anti-proliferative effects and reveals a 22.29 µg/mg•citrullin concentration in WRE as quantified by LC-MS.

Differential Impact on Apoptosis and Senescence
In the present study, we focused on elucidating the effects of watermelon rind extract (WRE) on various cellular processes, including cell proliferation, apoptosis, senescence, and global transcriptomic alterations in HRAC-769-P cells in vitro.Our findings predominantly indicate that WRE initiates apoptosis rather than senescence in these cells.The poly caspase FLICA probe, FAM-VAD-FMK, utilized in our study, is instrumental in detecting the early stages of apoptosis by identifying activated caspases.Conversely, senescence-associated beta-galactosidase (SA-beta-gal) is an established marker for cell senescence [16].Our results showed that SA-beta-gal activity remained unchanged or decreased upon treatment with WRE, underscoring a limited role for senescence in this context.These findings confirm that apoptosis and senescence are exclusive cellular outcomes [17].

The Intricacies of Nitric Oxide Signaling
NO is a versatile signaling molecule that induces apoptosis in cancer cells through various mechanisms, including the caspase cascade [18].Additionally, NO can interact with reactive oxygen species (ROS) to produce peroxynitrite, which has been shown to inflict cellular damage and promote apoptosis [19].In our study, elevated citrulline levels could be postulated to enhance NO production, thereby triggering apoptosis in HRAC-769-P cells (Figure 6).However, the influence of NO in cellular mechanisms can be complex and context-dependent, necessitating further investigation.Apart from citrulline, alternative mechanisms might involve the inhibition of mitochondrial respiration, changes in cytochrome c levels, or the suppression of anti-apoptotic genes like bcl−2.In summary, while our study establishes a promising link between WRE and induced apoptosis in HRAC-769-P cells, the precise mechanisms and the potential for therapeutic applications remain to be fully explored.The implications of citrulline and NO on different cancer types and cellular contexts also warrant comprehensive investigation.

Watermelon Rind Extract Modulates a Complex Network of Genes
Our study offers valuable insights into the transcriptomic landscape modulated by watermelon rind extract (WRE) in HRAC-769-P cells.The data illuminate how WRE affects various genes that could influence cell proliferation and apoptosis.This complexity underscores the necessity for a systems biology approach to understand the multifaceted gene networks regulated by WRE.

Promising Effects of Upregulated Transcripts on Tumor Growth Inhibition
Our transcriptomic data align with existing studies that have identified genes like NPTX1, TRIM31, and CD82/KAI1 as potential therapeutic targets in various cancers [25][26][27][28][29][30][31][32][33].NPTX1 has been implicated in inhibiting tumor growth across multiple types of cancer, acting through distinct signaling pathways.Similarly, TRIM31 is a tumor suppressor gene in breast and ovarian cancer.ZC3H12A exhibits tumor-suppressive effects in colorectal cancer (CRC) by inducing apoptosis, inhibiting angiogenesis, and EMT signaling.Its expression is associated with chemokine ligands, indicating a potential role of immune

Watermelon Rind Extract Modulates a Complex Network of Genes
Our study offers valuable insights into the transcriptomic landscape modulated by watermelon rind extract (WRE) in HRAC-769-P cells.The data illuminate how WRE affects various genes that could influence cell proliferation and apoptosis.This complexity underscores the necessity for a systems biology approach to understand the multifaceted gene networks regulated by WRE.

Multiple Gene Targets and Potential Therapeutic Avenues
Beyond the well-studied genes, our data indicated the potential roles of several other genes, including PAQR5, TRIM16, and ZC3H12A, in modulating cancer cell behavior [34,35,[63][64][65][66].These genes showed significant changes in expression upon WRE treatment, indicating their role in the anticancer effects of WRE.However, their specific anticancer mechanisms require further investigation.

Plant Material and Extraction Process
A fresh fruit of watermelon (Citrullus lanatus) was used in the study.The fruit rind or mesocarp was separated and dried.One kg of the dried rind was then finely powdered using a blender, and 100 g of it was dissolved in 70% ethanol in four separate 500 mL aliquots.The derived mixture was filtered using a 0.22 µm bottle top filter (500 mL-CELLTREAT Scientific Products).The filtrate was subjected to evaporation at 50 • C for a few hours to remove the ethanol.The extracted powder was initially dissolved in 0.1% ethanol, and the remaining volume was made up with water, sterilized by filtration, and preserved at −20 • C. The obtained samples were used for various biological and chemical investigations.

Chemical Characterization of Watermelon Rind Extract Using LC-MS
The phytoconstituents of WRE were identified using LC-MS.One mL methanol and 5 µL internal standard (4-Chloro-DL-phenylalanine, 25 µg/mL) were added to the 100 mg•WR sample.This was followed by vortexing for 1 min, centrifugation for 15 min at 20,000 rcf, and 100 µL of liquid fraction was transferred into a glass vial for subsequent analysis.For LC-MS metabolite profiling, a 5 µL sample was injected into the instrument.Samples were analyzed utilizing a Dionex Ultimate 3000 series UHPLC system (Thermo Scientific, Waltham, MA, USA) with a Q-Exactive MS system (Thermo Scientific, Waltham, MA, USA), as described previously [72].Metabolite assignments were made with citrulline as the target and also as an untargeted LC-MS metabolite profiling assay.
When the cells reached around 90% confluence, the cells were sub-cultured by splitting them at ratios ranging from 1:4 to 1:12.To achieve this, a 0.25% Trypsin/0.53mM EDTA solution in Hank's balanced salt solution (HBSS) without Ca 2+ and Mg 2+ (ATCC) was used for 10 min at 37 • C.

Treatments
Different concentrations of stock solutions of WRE were prepared in nuclease and microbial-free water, filter-sterilized, and stored at −20 • C. A stock media for various assays was prepared by adding RPMI-1640 media without phenol red (ATCC) along with FBS (10%), L-glutamine (0.3 g L −1 ; Gibco), and antibiotic-antimycotic solution (1%).The 50% inhibitory concentration (IC50) was considered from dose-response curves using GraphPad Prism 8.4.2 (Supplementary Figure S2).The IC50 value (88.6 mg•mL −1 ) and another concentration, 44.8 mg•mL −1 , were combined with the stock media for assays.Corresponding controls were also prepared by mixing similar volumes with the stock media.

Cell Proliferation Assay
Cell proliferation was noted with the WST-8 cell proliferation assay kit (CCK8, Dojindo, Kumamoto, Kyushu, Japan) by using an orange fluorescent dye.WST-8 will be reduced by dehydrogenases abundant in viable cells and transformed to formazan, an orange-colored dye soluble in the culture medium.The amount of formazan dye produced by the activity of dehydrogenases in cells is directly correlated with the number of viable cells.Five hundred live cells were supplemented to each well, holding 100 µL culture media in a 96-well flat black-bottomed plate (Greiner, Monroe, NC, USA).After 24 h of treatment, culture media was substituted with freshly prepared control or treatment media.Plates were collected by gently removing the culture media after 24, 48, and 72 h of treatment and kept at −80 • C before further analysis.Plates were allowed to thaw for 0.5 h at room temperature before starting the assay.In each well, 10 µL of WST-8 (1×) solution was added and thoroughly mixed using a multichannel pipette.The absorbance was then measured at 450 nm (excitation/emission) using a microplate reader (SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA).

Poly Caspase Assay
The process of apoptosis was performed using the Poly caspase assay kit (FAM FLICA, ImmunoChemistry Technologies, Davis, CA, USA).The probe FAM-VAD-FMK green fluorescent inhibitor binds to active cell caspase enzymes.Cells were grown in T25 flasks with phenol red-free media.Further cells were tested with different treatments when they reached 80% confluence.This study used staurosporine (6 µM) (ImmunoChemistry Technologies, Bloomington, MN, USA.) as a positive control.At various time intervals (0.5, 1, 2, and 4 h), floating cells were collected with media in a 15 mL centrifuge tube at 5000× g for 5 min at room temperature.The supernatant was dispensed; cells were suspended with 600 µL of 1× apoptosis wash buffer.Leftover adhered cells on the flask were spooled with trypsin and added to the cell suspension after removing the supernatant.The poly caspase inhibitor FAM-FLICA (1×) was added to the 500 µL cell suspension and allowed for incubation at 37 • C for one hour with intermittent shaking.Simultaneously, some of the remaining cells were utilized to count cell numbers with a hemacytometer using trypan blue (0.04%).Subsequently to the incubation, cells were washed with 2 mL of wash buffer, centrifuged, and the supernatant was eliminated.Following the washing step, the cells were incubated at 37 • C for 12 min to remove any excess FAM-FLICA reagent.Subsequently, the cells were collected through centrifugation, suspended in 500 µL of wash buffer, and kept on ice.Ultimately, 100 µL of cell suspension was utilized for assessing polycaspase activity in 96-well flat black bottom plates.The optical density (fluorescence) was measured in the microplate reader at 488/520 nm (excitation/emission), and the resulting RFU (Relative Fluorescence Units) values were normalized.

SA-Beta-Gal Assay
Senescence assay was carried out with SA-beta-gal activity with the 96-well cell senescence assay kit (Cell Biolabs, San Diego, CA, USA).The procedure is almost similar to poly-caspase assay except for a few modifications.The activity readings were measured at 0.5, 1, and 2 h.Following the cell collection, they were washed with PBS (phosphatebuffered saline) after the treatment.Subsequently, the cells were suspended in 400 µL of cell lysis buffer (1×) and kept on ice for 10 min.Further, the cell suspension was dissolved thoroughly by vertex, and 100 µL solution was taken out from this and frozen at −80 • C.Moreover, the leftover solution was utilized for the WST-8 cell proliferation assay.A 200 µL solution was mixed with one volume of 2× reaction buffer containing SA-beta-gal substrate and grown at 37 • C for 60 min in the dark.The incubated solution was adequately mixed, and a 200 µL solution was combined with an 800 µL stop solution.A 200 µL solution detected the SA-beta-gal activity in 96-well flat black-bottom plates.The optical density based on fluorescence was measured using a microplate reader at 360/465 nm (excitation/emission), and the resulting RFU (Relative Fluorescence Units) values were normalized or standardized to those obtained from the WST-8 cell proliferation assay.

Wound Healing Assay
The wound healing assay evaluated the effects of cell migration inhibition and metastasis.HRAC-769-P cells were cultured in 6-well plates until reaching 80-90% confluence.Subsequently, uniform scratches/wounds were created in each well using a 20 µL pipette tip.Afterward, the cells were carefully washed with sterile PBS to remove debris and treated with the WRE.The progress of wound closure was observed immediately (0 h) and after 48 h using an inverted microscope (DFC290, Leica, Wetzlar, Germany).Cells were stained with equal volumes of 1% toluidine blue and 1% borax (LabChem Inc., Zelienople, PA, USA) for photography.The experiments were conducted in triplicate to ensure reliability and reproducibility, as described by [73].

RNA Isolation and RNA-Seq Library Preparation
Total ribonucleic acid of control and treatment was extracted from the tissues of biological replicates using RNeasy Plus Mini Kit (QIAGEN, Germantown, MD, USA).The RNA's quality and quantity were assessed using the bioanalyzer Agilent 2100 and Qubit 4 Fluorometer (Invitrogen, Waltham, MA, USA), respectively.The RNA sequencing libraries were developed using the NEBNext Ultra™ II RNA Library Prep Kit, following the manufacturer's protocol provided by NEB, USA.The mRNAs were enriched using Oligo (dT) beads, and subsequently, they were fragmented into shorter fragments using fragmentation buffer.The first-strand cDNA was synthesized from the fragmented mRNA using random hexamer primers and later converted into double-strand cDNA.The resulting double-strand cDNAs were end-repaired and added with Illumina sequencing adapters.The adapter-ligated libraries were amplified using sequencing primers for enrichment.The library's quality and insert size were determined using a bioanalyzer (Invitrogen, Waltham, MA, USA), and the library was estimated using a Qubit fluorometer (Invitrogen, Waltham, MA, USA).The library was diluted to 4 nM concentration and sequenced using Illumina's NextSeq 500 platform with paired-end sequencing chemistry.The resulting image files in the BCL format were converted to FASTQ with 2 × 150 bp reads using the bcl2fastq tool (Illumina, San Diego, CA, USA).

RNA-Seq Analysis
In the analysis, sequencing adapters and low-quality reads (Phred score QV < 30) were removed using Trimmomatic v. 0.39 [74].The quality-filtered reads were then mapped to the Human (GRCh38.p13)reference genome (https://www.gencodegenes.org/human/;accessed on 15 April 2023) using STAR RNA-Seq aligner v. 2.7.11a [75] to produce BAM alignment.A read count table was created from the BAM alignment file and genome annotation in GFF format using the HTSeq R package [76].Differential gene expression (DEG) analysis was carried out using DESeq2 [77], filtering DEGs based on a minimum log2FoldChange of 1 and a false discovery rate (FDR) of 0.05.Pathway Enrichment analyses were conducted using KOBAS (http://bioinfo.org/kobas;accessed on 24 April 2023).

Conclusions
Our study reveals that watermelon rind extract (WRE) holds high proportions of nutraceutical components and amino acid derivatives like citrulline and arginine.It demonstrates a dose-and time-dependent reduction in HRAC-769-P cell proliferation in vitro.The decrease in cell proliferation is primarily attributed to apoptosis, supported by the upregulation of early poly-caspase activities and either normal or suppressed SA-betagal activity.Transcriptomic analyses suggest that the apoptotic effects may be mediated through both intrinsic and extrinsic pathways involving various key genes and molecular mechanisms.While our study presents a detailed overview, further in-depth research is required to validate these findings and explore clinical applications.

Figure 4 .
Figure 4. Effect of WRE (88.6 mg•mL −1 ) on cell migration in cells (A) Scratching was performed with a 20 µL pipette tip.Quantitative representation of the migration of kidney cancer cells by the wound healing assay.(B) The data are presented as the mean and standard deviation.The one-way ANOVA test was used to examine statistical differences.* Significant at p < 0.05, ** significant at p < 0.001, *** significant at p < 0.0001.

Figure 4 .
Figure 4. Effect of WRE (88.6 mg•mL −1 ) on cell migration in cells (A) Scratching was performed with a 20 µL pipette tip.Quantitative representation of the migration of kidney cancer cells by the wound healing assay.(B) The data are presented as the mean and standard deviation.The one-way ANOVA test was used to examine statistical differences.* Significant at p < 0.05, ** significant at p < 0.001, *** significant at p < 0.0001.

17 Figure 5 .
Figure 5. Volcano plot of differentially expressed genes (DEGs).The volcano plots from control vs. treatment conditions 44.8 mg•mL −1 .The volcano plot illustrates the association between the fold change (log2) and statistical significance (−log10(p-value)) of DEGs.The log2 fold change is represented along the x-axis, with upregulated genes to the right and downregulated genes to the left.The y-axis represents the −log10(p-value), with more significant DEGs at the top of the graph.Points on the graph represent individual genes, with color-coding indicating the significance level and fold change: red points represent significantly upregulated genes, blue points represent significantly downregulated genes, and gray points represent non-significant genes.

Figure 5 .
Figure 5. Volcano plot of differentially expressed genes (DEGs).The volcano plots from control vs. treatment conditions 44.8 mg•mL −1 .The volcano plot illustrates the association between the fold change (log2) and statistical significance (−log10(p-value)) of DEGs.The log2 fold change is represented along the x-axis, with upregulated genes to the right and downregulated genes to the left.The y-axis represents the −log10(p-value), with more significant DEGs at the top of the graph.Points on the graph represent individual genes, with color-coding indicating the significance level and fold change: red points represent significantly upregulated genes, blue points represent significantly downregulated genes, and gray points represent non-significant genes.

Figure 6 .
Figure 6.Possible mechanisms of WRE-mediated cell death in HRAC-769-P cells.WRE contains a high amount of citrulline and arginine.L-arginine is produced from L-citrulline to L-arginine by arginine succinate synthease and lyase.Meanwhile, cytokine-induced nitric oxide synthase (iNOS or NOS2) converts arginine into nitric oxide while material is recycled.Nitric oxide can influence survival pathways in cancer cells, leading to a shift in the balance between cell survival and cell death signals in favor of apoptosis.WRE induces apoptosis by regulating the expression of critical genes involved in several cellular pathways, molecular mechanisms, and metabolism.Enzymatic assays and transcriptomic analysis suggested that WRE-induced apoptosis in HRAC-769-P cells was mediated through intrinsic and extrinsic apoptotic pathways and by inhibiting the NF Kappa B pathway and induction of BMF, suppressing anti-apoptotic genes.

Figure 6 .
Figure 6.Possible mechanisms of WRE-mediated cell death in HRAC-769-P cells.WRE contains a high amount of citrulline and arginine.L-arginine is produced from L-citrulline to L-arginine by arginine succinate synthease and lyase.Meanwhile, cytokine-induced nitric oxide synthase (iNOS or NOS2) converts arginine into nitric oxide while material is recycled.Nitric oxide can influence survival pathways in cancer cells, leading to a shift in the balance between cell survival and cell death signals in favor of apoptosis.WRE induces apoptosis by regulating the expression of critical genes involved in several cellular pathways, molecular mechanisms, and metabolism.Enzymatic assays and transcriptomic analysis suggested that WRE-induced apoptosis in HRAC-769-P cells was mediated through intrinsic and extrinsic apoptotic pathways and by inhibiting the NF Kappa B pathway and induction of BMF, suppressing anti-apoptotic genes.

Table 1 .
Tentatively identified metabolites via LC-MS from watermelon rind aqueous extract using negative ionization mode.Rt: retention time.

Table 2 .
Watermelon rind extract-treated HRAC-769-P cells induced transcripts (upregulated and downregulated) mapped to human genome mapping percentage of uniquely mapped reads.

Table 2 .
Watermelon rind extract-treated HRAC-769-P cells induced transcripts (upregulated and downregulated) mapped to human genome mapping percentage of uniquely mapped reads.

Table 3 .
Differentially expressed transcripts of HRAC cells treated with WMR.Fold change of a transcript is a ratio of its expression in control and WMR-treated cells (n = 3).The fold change indicates up-(highlighted in blue) or downregulation (highlighted in green) of that transcript, respectively, in WRE-treated cells compared with control.

Table 3 .
Differentially expressed transcripts of HRAC cells treated with WMR.Fold change of a transcript is a ratio of its expression in control and WMR-treated cells (n = 3).The fold change indicates up-(highlighted in blue) or downregulation (highlighted in green) of that transcript, respectively, in WRE-treated cells compared with control.