Development of Novel Nano-Sized Imine Complexes Using Coriandrum sativum Extract: Structural Elucidation, Non-Isothermal Kinetic Study, Theoretical Investigation and Pharmaceutical Applications

A new Schiff base (H2L) generated from sulfamethazine (SMT), as well as its novel micro- and nanocomplexes with Ni(II) and Cd(II) metal ions, have been synthesized. The proposed structures of all isolated solid compounds were identified with physicochemical, spectral, and thermal techniques. Molar conductance studies confirmed that the metal complexes are not electrolytic. The molecular geometry located at the central metal ion was found to be square planar for the NiL2 and tetrahedral for the CdL2 complexes. The kinetic and thermal parameters were obtained using the Coats and Redfern approach. Coriandrum sativum (CS) in ethanol was used to create the eco-friendly Ni and Cd nanocomplexes. The size of the obtained nanoparticles was examined using PXRD and TEM, and found to be in the sub-nano range (3.07–4.61 nm). Furthermore, the TEM micrograph demonstrated a uniform and homogeneous surface morphology. The chemistry of the prepared nanocomplexes was studied using TGA and TEM techniques. The effect of temperature on the prepared nanocomplexes’ size revealed a decrease in size by heating. Furthermore, the nanocomplexes’ antimicrobial and anticancer properties were evaluated. The outcomes demonstrated that the nanocomplexes exhibited better antimicrobial properties. Moreover, the antitumor results showed that after heating, the Ni nanocomplex exhibited a substantial antitumor activity (IC50 = 1.280 g/mL), which was higher than the activity of cis-platin (IC50 = 1.714 g/mL). Finally, molecular-docking studies were performed to understand the evaluated compounds’ ability to bind to methionine adenosyl-transferases (PDB ID: 5A19) in liver cancer and COVID-19 main protease (PDB ID: 6lu7) cell-proteins. The findings reveal that [NiL2]·1.5H2O2 has a higher binding energy of −37.5 kcal/mol with (PDB ID: 5A19) cell protein.


Introduction
Schiff bases are formed through the condensation of primary amines and carbonyl compounds to generate imines or azomethine groups [1].Schiff bases are important in the synthesis of Schiff base complexes because these ligands have the ability to form stable compounds with metal ions [2].In addition to this, Schiff bases and their complexes have a broad range of biological activities [3,4].
On the other hand, sulfonamides, generally referred to as sulfa medicines, were the first routinely used chemotherapeutics for treating and preventing bacterial infections in humans [5].Due to their uses, a wide variety of families of organic and inorganic compounds are now being studied.One of the most common compounds is sulfonamide and its N-derivatives [6].Numerous Schiff bases derived from sulfonamide have been synthesized and used as chelates in the synthesis of strong metal chelates.Sulfamethazine is a sulfa-based antibacterial drug used for treating livestock diseases [7].Like other sulfonamides, sulfamethazine has been modified through the creation of Schiff bases [8] and metal complexes [9].The creation of nano-sized composites results in the synthesis of new compounds with distinctive physical, chemical, and biological characteristics [10].
Because of the huge number of surface atoms relative to atoms, nanoparticles (1-100 nm) generated by nanotechnological processes exhibit various novel physicochemical features in comparison to macro-sized structures [11,12].Many studies on the creation of nanoparticles, which have a wide range of uses, have lately been carried out, and significant findings have been obtained.Although the physical and chemical technologies employed in nanoparticle synthesis enable the manufacture of nanoparticles of any size in a short amount of time, their toxicity is high [13].In order to obtain harmless types of nanoparticles, it is necessary to employ newer and more practical techniques The green synthesis of nano-metal-complexes offers an alternative, effective, low-cost, and eco-friendly way to create nanoparticles with defined geometries [14,15].Researchers have succeeded in creating different nanoparticles for this purpose through the use of plant extracts and microorganisms [16].The manufacture of nanoparticles using plants has not received much attention, according to a review of the relevant scientific literature.The production of nanoparticles from plant extracts is faster than the microbial synthesis, and the resulting nanoparticles have a more stable structure [17].
In this article, we have synthesized two novel micro-and nanocomposites with a ligand derived from sulfamethazine and 2-hydroxy-1-naphthaldehyde.Numerous methods, including physicochemical and analytical tools, were applied to describe the produced Schiff base and its micro-and nanocomposites.The chemistry of the generated nano-composites was studied using TGA and TEM techniques.Also, computational investigations, such as DFT and molecular docking, were carried out on the prepared compounds.The kinetics and thermodynamic parameters for some thermal decomposition steps have been critically studied.Moreover, the in vitro antibacterial, antifungal, and anticancer activities of the prepared compounds were examined.

Physical, Microanalytical, and Molar Conductance Data
According to the complex's molar conductance measurement, which was carried out in 10 −3 M DMF solvent and was between 0.7 and 12 ohm −1 cm 2 mol −1 , the complexes under examination have a non-electrolytic nature.The microanalytical data revealed that all metal chelates were synthesized with a 1:2 (M:L) stoichiometry (Table 1).

1 H-NMR
The 1 H NMR spectrum of the newly prepared H 2 L ligand was recorded in DMSO-d6 (Figure S1).The peaks appear at δ 2.21, δ 9.64, and δ 2.51 ppm, which may be assigned to the CH 3 , OH, and DMSO-d6 [18] protons.The proton of azomethine was detected at δ 8.58 ppm [18], confirming the production of the Schiff base ligand.A signal at δ 10.61 ppm is due to the proton of the NH group [19].The signals recorded between δ 7.24 and δ 7.91 ppm are characteristic of the aromatic protons [20].The chelation mode between the ligand and the metal ions may be established by comparing the complex IR spectra to those of the free ligand (Figure S2).The finding proves that the ligand behaves in a bidentate manner.The characteristic vibrational bands were identified at 3200, 3457, 1384, and 1156 cm −1 for the free H 2 L ligand, assigned to υ(OH), υ(NH), υ as (SO 2 ), and υ sy (SO 2 ) groups, respectively.The (HC=N) vibration of the ligand at 1633 cm −1 indicates the synthesis of the H 2 L ligand; however, in the complexes of Ni(II) and Cd(II), this peak migrated to 1602 and 1624 cm −1 , due to its coordination [21].In the Ni(II) and Cd(II) complexes, the phenolic (O-H) of the H 2 L vanished, demonstrating proton transfer during complex formation.The persistence of the υ(NH) at 3457, υ as (SO 2 ) at 1384 cm −1 , and υ sy (SO 2 ) at 1156 cm −1 in the spectra of the complexes and their free ligand rules out the coordination through these groups [22].Finally, the peaks observed for the metal chelates at 544-549 and 566-585 cm −1 were assigned to (metal-nitrogen) and (metal-oxygen), respectively.Moreover, the band at 970 in the spectrum of the Ni(II) chelate could be ascribed to the (H 2 O) [23] (Table S1).

IR Spectroscopy of Nano-Complexes
The binding modes of the Ni and Cd nanocomposites produced in CS/EtOH media (Figure S3) are revealed by the FTIR spectra.This is corroborated by a shift in the peak location of the nanocomposites as compared to the free ligand.

Mass Spectra
Mass spectrometry has been used successfully to confirm the H 2 L Schiff base's molecular ion peaks and its metal complexes.The observed mass spectrum of the H 2 L ligand indicates a molecular ion peak (M + ) at 432.50, which strongly supports the proposed formula.The fragment pattern of the H 2 L ligand provides an impression of the possible deterioration, with a sequence of peaks corresponding to different fragments.The suggested fragmentation pattern of the H 2 L is given in a supplementary data file (Scheme S1).The MS of Ni(II) and Cd(II) complexes showed molecular ion peaks at 948.73 and 975.79, respectively, which suited the molecular weights of these complexes.They also showed that Ni and Cd isotope peaks were present at m/z 60 and 114, respectively (Figure 1).

XRD of Nanocomplexes
The obtained Ni and Cd nanocomplexes in CS/EtOH media were examined by th powder XRD method (Figure 2).XRD patterns suggested that the prepared nanocom plexes lie between amorphous and crystalline characters.As a result, it was found tha nanocomplexes were unsuitable for single-crystal XRD.The peak broadening indicated that the prepared compounds are nanoscale in size [24].The XRD pattern shows that th crystal size can be calculated according to the Debye-Scherrer formula [18,25].The av erage crystal sizes of Ni and Cd nanocomplexes are 33.1 and 36.4 nm, respectively.

XRD and Morphological Studies 2.5.1. XRD of Nanocomplexes
The obtained Ni and Cd nanocomplexes in CS/EtOH media were examined by the powder XRD method (Figure 2).XRD patterns suggested that the prepared nanocomplexes lie between amorphous and crystalline characters.As a result, it was found that nanocomplexes were unsuitable for single-crystal XRD.The peak broadening indicated that the prepared compounds are nanoscale in size [24].The XRD pattern shows that the crystal size can be calculated according to the Debye-Scherrer formula [18,25]

XRD of Nanocomplexes
The obtained Ni and Cd nanocomplexes in CS/EtOH media were examined by the powder XRD method (Figure 2).XRD patterns suggested that the prepared nanocomplexes lie between amorphous and crystalline characters.As a result, it was found that nanocomplexes were unsuitable for single-crystal XRD.The peak broadening indicated that the prepared compounds are nanoscale in size [24].The XRD pattern shows that the crystal size can be calculated according to the Debye-Scherrer formula [18,25].The average crystal sizes of Ni and Cd nanocomplexes are 33.1 and 36.4 nm, respectively.

TEM Analysis
The morphology and size of Ni and Cd nanoparticles created in Coriandrum sativum (CS) media were studied, as well as the impact of heat on the prepared nanocomplexes after heating them at 200 • C for two hours using the TEM technique (Figure 3).The images demonstrate particle morphologies to be homogeneous and close together, which is evidence that there are identical matrices.The mean particle size of Ni and Cd nanocomplexes before heating was about 24.55 and 33.89 nm, respectively.However, after heating, the Ni and Cd nanocomposites at 200 • C were 4.61 and 3.07 nm (sub-nano), respectively, indicating that heating reduces the particle size of the formed nanocomplexes.These findings accord well with the estimated crystallite size values from the XRD technique.

TEM Analysis
The morphology and size of Ni and Cd nanoparticles created in Coriandrum sativum (CS) media were studied, as well as the impact of heat on the prepared nanocomplexes after heating them at 200 °C for two hours using the TEM technique (Figure 3).The images demonstrate particle morphologies to be homogeneous and close together, which is evidence that there are identical matrices.The mean particle size of Ni and Cd nanocomplexes before heating was about 24.55 and 33.89 nm, respectively.However, after heating, the Ni and Cd nanocomposites at 200 °C were 4.61 and 3.07 nm (sub-nano), respectively, indicating that heating reduces the particle size of the formed nanocomplexes.These findings accord well with the estimated crystallite size values from the XRD technique.The TG-DTG thermograms of Ni(II) and Cd(II) chelates are shown in Figure 4.The TG curve of the Ni(II) chelate exposed four decomposition steps.The first step from 52 to 111 • C with an estimated mass loss of 2.32% (calc.2.84%) corresponds to the removal of the non-coordinated H 2 O molecules.The second step, from 265 to 321 • C with a mass loss of 20.93% (calc.22.58%), corresponds to the removal of 2C 6 H 8 N 2 .The third decomposition step occurred at temperatures ranging from 404 to 566 • C with a mass loss of 21.70% (calc.22.79%), corresponding to the loss of SO 2 and 2C 6 H 6 .At a higher temperature of 727-767 • C, a mass loss of 30.01%(calc.30.18%) is attributed to the elimination of 2C 10 H 7 O.The TG curve of the Cd(II) chelate exposed a three-step decomposition.The Cd(II) complex showed no mass loss up to 271 • C, confirming the absence of coordinated water and the complex's high thermal stability.The mass losses in temperature from 271 to 318 • C with a mass loss of 21.69% (calc.21.96%) may be attributed to the decomposition of 2C 6 H 8 N 2 followed by the loss of 2C 6 H 6 , SO 2 and 2HCN with a mass loss of 26.19% (calc.27.71%) within the temperature from 420 to 481 • C.After this decomposition, the mass loss of the third decomposition stage was within the range from 772 to 811 • C with a mass loss of 30.42% (calc.29.14%), corresponding to the removal of the rest of 2C 10 H 7 O.In Ni(II) and Cd(II) chelates, metal oxides were left as a residue.

TG-DTG of Nanocomplexes
The thermogram of nickel and cadmium nanodomain metal chelates prepared in Coriandrum sativum (CS) extract in 20% ethanol aims to discover the chemistry of nanodomain metal chelates by evaluating the size of the nanodomain metal chelates at each step of thermal heating to ascertain how the heat affects the size of the nanodomain metal chelates (Figure 5).The thermograms of Ni and Cd nanodomain were studied before and after heating the nanodomain chelates at 200 • C for 2 h.The mean particle size of Ni and Cd nano-chelates before heating was about 10.11-21.39 and 12.90-31.96nm, respectively.However, after heating, the Ni and Cd nano-chelates at 200 • C were 2.05-39.6nm (sub-nano) and 1.08-2.78nm (sub-nano), respectively, indicating that heating reduces the particle size of the formed nano-chelates (Tables 2 and 3).

Kinetic Calculations
In the present work, the kinetic and thermodynamic parameters, the energy of activation (Ea), the activation enthalpy (∆H*), the entropy of activation (∆S*), the Gibbs energy change (∆G*), the order of the reaction (n), the correlation coefficient (r), and the pre-exponential factor (Z) for the non-isothermal decomposition of the prepared metal complexes were determined by the integral method proposed by Coats and Redfern [26].The obtained data are listed in Table 4. Using the following equations, the activation enthalpy (∆H*), the activation entropy (∆S*), and the free energy (∆G*) were determined.
The high E a values illustrate that the H 2 L is powerfully bonded to the Ni(II) and Cd(II) ions.The existence of negative ∆S* values in NiL 2 (first, second, and third steps) and CdL 2 (first step) demonstrates that the activated complexes are more ordered and the reactants are slower [27].The positive activation enthalpy ∆H* data refer to the endothermic decomposition process.The positive values of ∆G* mean that the decomposition reaction is not spontaneous [28].Figure 6 shows the plots of ln vs. 1/T for different models of Ni(II) and Cd(II) chelates, respectively, demonstrating that all models exhibit a linear trend with a good correlation coefficient.

UV-Visible Absorption Study
The electronic spectra of the prepared compounds were noted in DMF ( 10

UV-Visible Absorption Study
The electronic spectra of the prepared compounds were noted in DMF (10 −4 M) at room temperature.The free ligand demonstrated that absorption bands appear at 386, 445, and 468 nm due to the π→π* transition of the aromatic ring, n→π* of azomethine (HC=N), and n→π* of the phenolic group, respectively.Additionally, the band appearing at 539 nm may be due the charge-transfer (CT).The Ni(II) chelates exhibit three distinct bands at 365, 408, and 542 nm, attributed, respectively, to the transitions n→π* of azomethine (HC=N), 1 A 1g → 1 B 1g , and 1 A 1g → 1 A 2g for the square planar Ni(II) chelate [29].The electronic spectrum of the Cd(II) chelate demonstrated bands due to n→π* transition of azomethine (HC=N), which appeared at 456 nm.The second band is observed at 525 nm, which may be due to the CT transition [18,30].The electronic spectrum of green-synthesized Ni and Cd nanocomplexes displayed a band related to the surface plasmon resonance (SPR) [31], proving the formation of nanocomposites.The Ni nanocomplexes exhibit bands at 363, 399, and 535 nm, attributed, respectively, to the transitions n→π* of imine (C=N), 1 A 1g → 1 B 1g , and 1 A 1g → 1 A 2g for the square planar Ni nanocomplex.The spectra of the Cd nanocomplex show absorption bands at 406 nm which can be assigned to the imine (C=N) group.Finally, there is an absorption band at 513 nm, which may refer to a charge-transfer band caused by a change in electron distribution between the metal and a ligand.The spectra of Ni(II) and Cd(II) chelates and their nanocomplexes are shown in the supporting information (Figure S4), and the electronic spectral data are shown in Table 5.The most appropriate structural configuration of the H 2 L ligand is shown in Figure 7.The charges from the natural bond orbital (NBO) analysis are (−0.693),(−0.953), (−0.924), (−0.531), (−0.528), (−0.891), (−0.427), and (2.357) for O1, O2, O3, N1, N2, N3, N4, and S, respectively.This supports the coordination of metal ions with O3 and N4 in a bidentate manner.

Molecular Orbitals (MO) and MEP Maps
The computed energies of formation, HOMO (eV), LUMO (eV), as well as other chemical parameters connected to HOMO and LUMO energies, were determined for the H2L ligand and its metal chelates (Table 7).As reported elsewhere, HOMO energy measures the electron donation character, while LUMO energy measures the electron affinity [32].Higher stability compared to free ligand is shown by more negative metal chelate formation energy values [33].The chelating agent's computed (Eg) is greater than that of the Ni(II) and Cd(II) chelates, demonstrating its affinity for binding to the Ni(II) and Cd(II) ions [34].The extraordinarily low dipole moment value of the Ni(II) complex

Molecular Orbitals (MO) and MEP Maps
The computed energies of formation, HOMO (eV), LUMO (eV), as well as other chemical parameters connected to HOMO and LUMO energies, were determined for the H 2 L ligand and its metal chelates (Table 7).As reported elsewhere, HOMO energy measures the electron donation character, while LUMO energy measures the electron affinity [32].Higher stability compared to free ligand is shown by more negative metal chelate formation energy values [33].The chelating agent's computed (E g ) is greater than that of the Ni(II) and Cd(II) chelates, demonstrating its affinity for binding to the Ni(II) and Cd(II) ions [34].The extraordinarily low dipole moment value of the Ni(II) complex may be caused by the two ligands canceling each other out due to their trans-orientation (Figures 9 and 10).On the other hand, MEP maps (Figure 11) were created to distinguish the electrostatic potential of positive, negative, and neutral zones using the colors blue, red, and green, respectively.The MEP contains positive zones around hydrogen atoms and negative zones over electronegative atoms (oxygen and nitrogen).may be caused by the two ligands canceling each other out due to their trans-orientation (Figures 9 and 10).On the other hand, MEP maps (Figure 11) were created to distinguish the electrostatic potential of positive, negative, and neutral zones using the colors blue, red, and green, respectively.The MEP contains positive zones around hydrogen atoms and negative zones over electronegative atoms (oxygen and nitrogen).

Antitumor Activity
The anticancer activity of the ligand H 2 L and its Ni and Cd nano-chelates generated in CS/EtOH (before and after) heating them was examined against the HepG-2 human cancer cell line.Cis-platin (IC 50 = 1.714 µg/mL) was used as the standard.Compounds with IC50 values of less than 5.00, 5.00-10.00,and 10.00-25.00µg/mL are categorized as strong, moderate, and weak antitumor agents, respectively [35].The growth of the HepG-2 cancer cells was generally inhibited by the investigated compounds in a concentrationdependent manner.Moreover, the examined nano metal chelates were more cytotoxic than the comparable free ligand.This could be attributed to the metal's redox-active center [36].Interestingly, the nano-sized Ni complex and Cd complex, after heating, displayed strong cytotoxicity compared with other compounds, whereas the Ni nanocomplex after heating exhibited antitumor activity (IC50 = 1.280 g/mL), which was higher than the activity of cisplatin (IC50 = 1.714 g/mL), and the Cd nanocomplex (before heating) displayed moderate antitumor activity (Figures 12 and 13).

Antitumor Activity
The anticancer activity of the ligand H2L and its Ni and Cd nano-chelates generated in CS/EtOH (before and after) heating them was examined against the HepG-2 human cancer cell line.Cis-platin (IC50 = 1.714 μg/mL) was used as the standard.Compounds with IC50 values of less than 5.00, 5.00-10.00,and 10.00-25.00μg/mL are categorized as strong, moderate, and weak antitumor agents, respectively [35].The growth of the HepG-2 cancer cells was generally inhibited by the investigated compounds in a concentration-dependent manner.Moreover, the examined nano metal chelates were more cytotoxic than the comparable free ligand.This could be attributed to the metal's redox-active center [36].Interestingly, the nano-sized Ni complex and Cd complex, after heating, displayed strong cytotoxicity compared with other compounds, whereas the Ni nanocomplex after heating exhibited antitumor activity (IC50 = 1.280 g/mL), which was higher than the activity of cis-platin (IC50 = 1.714 g/mL), and the Cd nanocomplex (before heating) displayed moderate antitumor activity (Figures 12 and 13).

In Vitro Antimicrobial Results
The newly synthesized Schiff base ligand (H2L) and its Ni and Cd nanocomplexes (before and after) heating were investigated for their inhibitory effects on the growth of Bacillus cereus (G+ve), E. coli (G−ve), Micrococcus luteus (G+ve), Pseudomonas aeruginosa (G−ve), Serratia marcescens (G−ve), and Staphylococcus aureus (G+ve) bacteria, and Aspergillus flavus, Candida albicans, Fusarium oxysporum, Geotrichum candidum, Scopulariopsis brevicaulis, and Trichophyton rubrum fungi.The antimicrobial activity was tested using the disk

In Vitro Antimicrobial Results
The newly synthesized Schiff base ligand (H 2 L) and its Ni and Cd nanocomplexes (before and after) heating were investigated for their inhibitory effects on the growth of Bacillus cereus (G+ve), E. coli (G−ve), Micrococcus luteus (G+ve), Pseudomonas aeruginosa (G−ve), Serratia marcescens (G−ve), and Staphylococcus aureus (G+ve) bacteria, and Aspergillus flavus, Candida albicans, Fusarium oxysporum, Geotrichum candidum, Scopulariopsis brevicaulis, and Trichophyton rubrum fungi.The antimicrobial activity was tested using the disk diffusion method; the clear zone of inhibition around each disk was measured (in mm) and compared to the known sensitive drugs: chloramphenicol (CHL) as an antibacterial drug and clotrimazole (CLO) as an antifungal drug.The findings suggest that the nanocomplexes exhibit greater activity as compared to the H 2 L ligand under the same experimental conditions.This would indicate that the chelation could improve the ability of a nanocomplex to cross a cell membrane, which can be described by Tweedy's chelation theory [37].From the data in Table 8, the nano-range complexes after heating displayed higher influences on the tested bacteria compared with other compounds, indicating that the decrease in particle size caused by heating increases the activities.In case of antifungal activity, Cd nanocomplexes after heating showed good antifungal results against A. flavus and F. oxysporum within the inhibition zone, at 28 and 20 mm, which are greater than those of the standard clotrimazole (24 mm) and (18 mm), respectively (Figures 14 and 15).The growth of bacterial pathogens on each concentration was checked to determine the minimum concentration that inhibits the growth of the organism.It is evident from Table 9 that the MIC value for the Ni nanocomplex after heating was 0.625 mg/mL for B. cereus, E. coli (G−ve), and Serratia marcescens (G−ve).Furthermore, the Ni nanocomplex after heating demonstrates antifungal activity against the tested fungi, but Trichophyton rubrum appeared as the most sensitive fungus among all fungi involved in this study (Table 9).

Molecular Docking Studies
Molecular docking is a potent method for analyzing the biological activity of target molecules and producing distinguishing structural features for the creation of novel therapeutics.Moreover, it is a common computational technique for determining binding sites with appropriate conformations and estimating binding affinity.To predict the possible binding modes at the active pockets, all prepared compounds were docked with methionine adenosyl-transferases (PDB ID: 5A19) and COVID-19 main protease viral protein (PDB ID: 6lu7) (Figures 16 and 17).The results demonstrated that the ligand's dominant interaction force was the H-acceptor, although other binding interactions, such as the H donor, also took place in complexes.A stronger interaction between the investigated compounds and receptors reflects more negative energy.As a result, the interaction ability towards the receptor was arranged as follows: in the case of (PDB ID: 5A19), NiL 2 > CdL 2 > H 2 L, whereas in the case of (PDB ID: 6lu7), CdL 2 > NiL 2 > H 2 L (Tables 10 and 11).

Materials and Equipment
In-depth information about the chemicals, tools, and processes used for the structural verification and applications can be found in Section S1.

Procedure of Schiff Base Synthesis
The tested H 2 L ligand was prepared by adding a solution of (2.7833 g, 10 mmol) Sulfamethazine sodium salt to a solution of 2-hydroxy-1-naphthaldehyde (1.7218 g, 10 mmol) in 20 mL EtOH.The reaction mixture was left under reflux for 4 h at 70 • C. The resulting yellow solid was then mixed with dilute hydrochloric acid (5%) to form a neutral solution.The yellow precipitate was recrystallized from EtOH and then dried in a vacuum (Scheme 1).

Procedure of Schiff Base Synthesis
The tested H2L ligand was prepared by adding a solution of (2.7833 g, 10 mmo Sulfamethazine sodium salt to a solution of 2-hydroxy-1-naphthaldehyde (1.7218 g, mmol) in 20 mL EtOH.The reaction mixture was left under reflux for 4 h at 70 °C.Th resulting yellow solid was then mixed with dilute hydrochloric acid (5%) to form a ne tral solution.The yellow precipitate was recrystallized from EtOH and then dried in vacuum (Scheme 1).Scheme 1.General route for the synthesis of the Schiff base ligand (H2L) and its metal complexes

Procedure of Metal Complex Synthesis
The metal chelates were prepared by refluxing a mixture of the H2L ligand in 20 m hot ethanol with a solution of NiCl2 .6H2O or CdCl2 .2.5H2O in a 1:1 molar ratio.The rea

Procedure of Metal Complex Synthesis
The metal chelates were prepared by refluxing a mixture of the H 2 L ligand in 20 mL hot ethanol with a solution of NiCl 2 •6H 2 O or CdCl 2 •2.5H 2 O in a 1:1 molar ratio.The reaction mixtures were kept under reflux for 4-6 h at 70 • C, filtered, washed with ethanol, and dried at room temperature (Scheme 1).

Procedure of Nanocomplex Synthesis
Nano domain compounds of Ni(II) and Cd(II) were created in a 1:1 molar ratio by adding Coriandrum sativum (CS) media extract in 20% ethanol, as explained previously in our publications [32,38].

DFT Studies
The Schiff base ligand and its Ni(II) and Cd(II) chelates were optimized with the Gaussian 09 program [39], by implementing the LANL2DZ basis set for Ni and Cd metals, while the 6-311++G basis set was used for the rest of the atoms [40].

Cytotoxicity Assay
The antitumor property of the examined compounds was measured on a microplate reader (Sunrise, Tecan, Inc., East Lyme, CT, USA) using 490 nm filters against the Hepatocel-lular carcinoma cell line, HepG-2 cells (ATCC No. HB-8064), and compared to the cis-platin (cis-diamminedichloroplatinium) as reference drug.The experiments were conducted in the tissue culture section of Al-Azhar University's Regional Institute for Mycology and Biotechnology in Cairo, Egypt.The IC 50 values were calculated using the graphed prism version [41].

Molecular Docking (MD)
Molecular docking studies were performed using the MOE2019 software.The output file of the Gaussian 09 software was used to convert the structures of the H 2 L ligand and its complexes into a PDB format file.The crystal structures of the target proteins for (PDB ID: 5A19) in liver cancer [43] and COVID-19 main protease (PDB ID: 6lu7) [44] were downloaded from the Protein Data Bank (https://www.rcsb.orgaccessed on 1 January 2020).For protein preparation, the bounded water molecules, the co-crystallized ligand, and the other cofactors were removed.In order to assign the charges and the parameters, an MMFF94x force field was used.The MOE site finder was used to produce the most probable protein binding sites.The energy of the protein molecules and the prepared compounds was reduced utilizing the energy minimization algorithm of the Molecular Operating Environment (MOE2019 software).The binding affinity between the protein and the generated compounds was determined through analyzing the binding energies of the top ten poses.By comparing the values of the binding-free energy and hydrogen bond lengths, the best scoring binding affinity could be determined.The estimated hydrogen bond lengths should not exceed 3.5 Å.

Conclusions
In this study, two types of nano-sized complexes have been synthesized that are characterized by various physicochemical and spectral analyses.The ligand H 2 L acts as a bidentate chelate by coordinating to the metal ions through NO donor atoms, forming square planar and tetrahedral complexes.Furthermore, the prepared complexes' kinetic and thermodynamic properties are evaluated.The particles of the complexes under investigation were sub-nanoscale, as revealed by the XRD and TEM results.The findings reveal a decrease in the size of nano metal chelates after heating.The DFT calculations of the prepared compounds confirm the experimental results.The antimicrobial activities of the ligand, H 2 L, and its nano metal chelates showed that the metal ion in the nanocomposites enhanced the antimicrobial activities in comparison to the free ligand.Also, all compounds under examination demonstrated a reduction in HEPG-2 cell growth, and heating the nanocomplexes increased their cytotoxic effects.The Ni nanocomplex exhibited substantial antitumor activity (IC50 = 1.280 g/mL), which was higher than the activity of cis-platin (IC50 = 1.714 g/mL).Additionally, a molecular docking study suggests that [NiL 2 ]•1.5H 2 O and CdL 2 have the most activity with (PDB ID: 5A19) and (PDB ID: 6lu7) cell-proteins, respectively.It is worth mentioning that there is a limitation for using the investigated compounds as drugs due to their lack of stability.Also, there is a need for further investigation of the toxicity of the prepared complexes towards normal cancer cells.

Figure 3 .
Figure 3. TEM images and histogram of particle size distribution for Ni and Cd nanocomplexes before heating (a,b) and after heating at 200 °C (c,d).

Figure 3 .
Figure 3. TEM images and histogram of particle size distribution for Ni and Cd nanocomplexes before heating (a,b) and after heating at 200 • C (c,d).

Figure 7 .
Figure 7. Geometry-optimized structure, dipole moment, and the charges on active centers of the H 2 L ligand.

Figure 9 .
Figure 9. Surface plots of HOMO − LUMO of the H2L ligand.

Figure 12 .
Figure 12.The cell viability of H2L and its nano metal chelates versus cis-platin drug.

Figure 13 .
Figure 13.In vitro antitumor activity (IC 50 ) of H 2 L and its nano metal chelates (before and after) heating against HepG-2 cells.

Figure 14 .
Figure 14.Antibacterial activity of H2L and its nanocomplexes.Figure 14.Antibacterial activity of H 2 L and its nanocomplexes.

Scheme 1 .
Scheme 1.General route for the synthesis of the Schiff base ligand (H 2 L) and its metal complexes.

Table 1 .
Analytical and physical data of the compounds studied.

Table 2 .
Thermoanalytical results and the particle size of Ni and Cd nanocomplexes before heating.

Table 3 .
Thermoanalytical results and the particle size of Ni and Cd nanocomplexes after heating.

Table 4 .
The kinetic and thermodynamic data of the thermal decomposition of complexes.

Table 5 .
UV-visible spectral data of H 2 L and its micro-and nanocomplexes.

Table 7 .
Computed chemical parameters of the ligand and its complexes.

Table 8 .
Antibacterial and antifungal activity (inhibition zone in mm) of chemical compounds.

Table 9 .
Antibacterial and antifungal activity (inhibition zone and MIC) of chemical compounds (mg/mL).

Table 9 .
Antibacterial and antifungal activity (inhibition zone and MIC) of chemical compounds (mg/mL).

Table 10 .
Docking interaction data for the ligand (H 2 L) and its complexes towards liver cancer protein (PDB ID: 5A19).
* The lengths of H-bonds are in brackets.

Table 11 .
Docking interaction data for the ligand (H 2 L) and its complexes towards COVID-19 main protease viral protein (PDB ID: 6lu7).
* The lengths of H-bonds are in brackets.