OLR1 Is a Pan-Cancer Prognostic and Immunotherapeutic Predictor Associated with EMT and Cuproptosis in HNSCC

Metabolism plays a critical role in cancer. OLR1 has been implicated in cardiovascular and metabolic disorders, while its association with tumorigenesis and tumor immunity remains poorly defined in the literature. We conducted comprehensive pan-cancer analyses based on the TCGA database to examine OLR1 expression and its prognostic implications. Correlations between OLR1 expression level and tumor immunity and immunotherapy were investigated by immune infiltration, enrichment, and TIDE analysis methods. Immunohistochemistry detected OLR1 expression in HNSCC. We used the GSEA method to explore the potential signaling pathways in which OLR1 is involved, and a correlation analysis to investigate the relationships between OLR1 and epithelial–mesenchymal transition (EMT) and cuproptosis. In addition, the effects of OLR1 knockdown on the EMT process, invasion, stemness, and cuproptosis of HNSCC cells were examined by scratch, Transwell, CCK8, sphere formation, and flow cytometry, while changes in related proteins were detected using the immunoblotting method. OLR1 is highly expressed in most cancers, and it is associated with patient prognosis. OLR1 expression positively correlates with immunosuppressive cell infiltration and immune checkpoint molecules, while being negatively associated with effector T cells. Moreover, significant correlations are observed between OLR1 expression and tumor mutation burden (TMB) and microsatellite instability (MSI) in some cancers. In HNSCC, OLR1 expression is related to advanced clinicopathological factors and unfavorable outcomes. Patients with high OLR1 expression levels are prone to experience immune escape and benefit less from immune checkpoint inhibitor (ICI) therapy. Moreover, OLR1 expression may affect EMT, stemness, and cuproptosis resistance outcomes. OLR1 is an immune-related prognostic biomarker with potential as a prognostic indicator for immunotherapy, and it may also be involved in regulating the EMT process and cuproptosis in HNSCC.


Introduction
In recent decades, cancer has become the leading cause of death, with an estimated 19.3 million new cancer cases and nearly 10.0 million cancer deaths occurring in 2020, and cancer is a significant barrier to improving life expectancy worldwide [1].Although the number of cancer survivors continues to increase in developed countries, mainly due to advances in early prevention and treatment modalities, the mortality rate of cancer patients worldwide still shows a remarkable increasing trend [2,3].Immunotherapy, exemplified by immune checkpoint inhibitor (ICI) therapy, is gradually becoming a therapeutic standard in the field of cancer treatment planning, in addition to radiotherapy and chemotherapy [4,5].Nonetheless, only a minority of patients benefit from it, and low treatment response rates and drug resistance are the greatest obstacles to the development of such therapies [6].Therefore, further research is urgently required to verify the efficacy of cancer immunotherapy in diverse cancer types [7].The epithelial-mesenchymal transition (EMT) process and cancer stem cells are widely regarded in the literature to be the main culprits in tumor initiation, recurrence, and metastasis [8], exerting a substantial influence on the efficacy of ICI therapy [9].Cuproptosis is a recently identified form of programmed cell death that is closely related to cellular metabolism [10,11].However, in the field of tumor immunity, fewer reports exist concerning the role of cuproptosis.Consequently, further empirical evidence is required to substantiate its implications in this domain.
As the research field evolves, scientific attention has been increasingly focusing on the common characteristics present across different human malignancies to better understand the underlying mechanisms of tumor development [12].A pan-cancer analysis, which examines the genes of multiple cancers and compares the differences in gene expression, has been widely used in the field to identify tumor molecular markers and signaling pathways.This, in turn, has improved our understanding of the molecular mechanisms underlying tumor initiation and development processes [13,14].
Emerging research has revealed a strong connection between metabolic disorders and tumor development processes [15].Oxidized low-density lipoprotein receptor 1 (OLR1), the receptor of oxidized low-density lipoprotein (ox-LDL), is mainly expressed in endothelial cells, macrophages, smooth muscle cells, platelets, fibroblasts, and neurons [16].The effects of OLR1 have been studied in metabolic and cardiovascular diseases [17]; however, it is also overexpressed in some cancers and involved in various tumorigenic processes.In breast cancer, upregulated OLR1 predicts poor prognosis and promotes migration [18,19].In pancreatic cancer, OLR1 promotes metastasis through c-MYC and HMGA2 [20].Additionally, in colon cancer, OLR1 overexpression drives glycolytic metabolism, leading to proliferation and chemoresistance [21].In addition, OLR1 has been implicated in the progression of esophageal cancer through autophagy [22,23].Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), a translated protein of OLR1, has also been implicated in cancer [24].In pancreatic cancer, LOX-1 is associated with EMT and metastasis processes [25], whereas LOX-1 knockdown can suppress colorectal cancer progression and metastasis [26].Moreover, LOX-1 is one of the most overexpressed proteins in PMN-MDSC, and LOX-1 + PMN-MDSC presents potent immunosuppressive activity [27,28].However, the precise role of OLR1 in different tumor types, including HNSCC, and its relevance to tumor immunity and immunotherapy remain unclear in the research.
In this study, we used multiple public databases to evaluate OLR1 expression and its relationship with prognosis in 33 cancer types, and to explore correlations between OLR1 expression levels and immune infiltration and immune checkpoint expression.Finally, a validation was performed in HNSCC, and the correlations of OLR1 expression levels with the EMT process, stemness, and cuproptosis were investigated.The present study aims to evaluate the potential of OLR1 as a promising biomarker for predicting the prognosis and treatment efficacy of ICI, while also highlighting its association with the EMT process and cuproptosis resistance in HNSCC.This study provides new insights to improve immunotherapy response rates.

Aberrant Expression and Prognostic Value of OLR1 in Pan-Cancer
To determine the significance of OLR1 in malignancies, OLR1 mRNA expression levels were initially scrutinized in 33 cancer types, using the comprehensive dataset obtained from The Cancer Genome Atlas (TCGA).The results show that OLR1 is markedly upregulated in the majority of cancers, with a few exceptions.Intriguingly, OLR1 expression was downregulated in DLBC, LUAD, LUSC, SARC, and TGCT compared to normal tissues.(Figure 1A).Furthermore, the paired comparative analysis showed that OLR1 expression was significantly higher in BRCA, CHOL, COAD, ESCA, HNSCC, KIRC, KIRP, PRAD, STAD, and THCA compared to adjacent normal tissues.In contrast, a reduced expression of OLR1 was observed in tumor tissues of LUAD and LUSC (Figure 1B).PRAD, STAD, and THCA compared to adjacent normal tissues.In contrast, a reduced expression of OLR1 was observed in tumor tissues of LUAD and LUSC (Figure 1B).To investigate the prognostic significance of OLR1 expression across 33 different types of cancer, we performed a survival analysis using a Cox proportional hazards model.Our results indicated that patients with elevated OLR1 expression levels experienced worse OS in CESC, COAD, HNSCC, LGG, SARC, STAD, THCA, and THYM.Conversely, in CHOL, LUAD, and SKCM, patients with high OLR1 expression levels had a better prognosis (Figure 2A).This observation was further validated by the Kaplan-Meier survival analysis (Figure 2B-K).Subsequently, we examined the correlations between OLR1 expression and PFI and DSS.Our analysis demonstrated that elevated OLR1 expression was closely associated with poor PFI in ACC, COAD, GBM, HNSCC, LGG, LUSC, PAAD, and PRAD; however, in BLCA, CHOL, DLBC, and SKCM, a high OLR1 expression level represented a better PFI outcome (Figure S1A).Similarly, the univariate Cox regression analysis of DSS outcomes indicated that high OLR1 expression was a risk factor for DSS in patients with ACC, COAD, ESCA, HNSCC, LGG, LUSC, PAAD, and UCEC; however, the opposite result was observed in CHOL, KIRP, LUAD, PCPG, and SKCM (Figure S1B).Collectively, our results suggest that OLR1 expression levels are significantly elevated in the progression of various cancers and have the potential to serve as a prospective pan-cancer prognostic biomarker, particularly for COAD, HNSCC, LGG, and CHOL.To investigate the prognostic significance of OLR1 expression across 33 different types of cancer, we performed a survival analysis using a Cox proportional hazards model.Our results indicated that patients with elevated OLR1 expression levels experienced worse OS in CESC, COAD, HNSCC, LGG, SARC, STAD, THCA, and THYM.Conversely, in CHOL, LUAD, and SKCM, patients with high OLR1 expression levels had a better prognosis (Figure 2A).This observation was further validated by the Kaplan-Meier survival analysis (Figure 2B-K).Subsequently, we examined the correlations between OLR1 expression and PFI and DSS.Our analysis demonstrated that elevated OLR1 expression was closely associated with poor PFI in ACC, COAD, GBM, HNSCC, LGG, LUSC, PAAD, and PRAD; however, in BLCA, CHOL, DLBC, and SKCM, a high OLR1 expression level represented a better PFI outcome (Figure S1A).Similarly, the univariate Cox regression analysis of DSS outcomes indicated that high OLR1 expression was a risk factor for DSS in patients with ACC, COAD, ESCA, HNSCC, LGG, LUSC, PAAD, and UCEC; however, the opposite result was observed in CHOL, KIRP, LUAD, PCPG, and SKCM (Figure S1B).Collectively, our results suggest that OLR1 expression levels are significantly elevated in the progression of various cancers and have the potential to serve as a prospective pan-cancer prognostic biomarker, particularly for COAD, HNSCC, LGG, and CHOL.

Immune Infiltration Analysis of OLR1 in Pan-Cancer
Increasing evidence suggests that cancer metabolism is not only involved in regulating tumor initiation and development, but also leads to the reprogramming of immune cell metabolic pathways, resulting in impaired anti-tumor immune responses [29].At present, targeting cancer metabolism has been shown in the research to synergistically enhance immunotherapy [30].Thus, we investigated the association between the metabolic gene OLR1 and tumor immunity in our study, aiming to ascertain its potential as a therapeutic target in cancer immunotherapy.The tumor microenvironment (TME) plays a crucial role in tumorigenesis and progression [31].In an immunosuppressed TME, tumor- show that overall survival is decreased in CESC, COAD, HNSCC, LGG, SARC, STAD, and THYM patients with higher OLR1 expression levels, whereas the opposite result is observed in CHOL, LUAD, and SKCM patients.

Immune Infiltration Analysis of OLR1 in Pan-Cancer
Increasing evidence suggests that cancer metabolism is not only involved in regulating tumor initiation and development, but also leads to the reprogramming of immune cell metabolic pathways, resulting in impaired anti-tumor immune responses [29].At present, targeting cancer metabolism has been shown in the research to synergistically enhance immunotherapy [30].Thus, we investigated the association between the metabolic gene OLR1 and tumor immunity in our study, aiming to ascertain its potential as a therapeutic target in cancer immunotherapy.The tumor microenvironment (TME) plays a crucial role in tumorigenesis and progression [31].In an immunosuppressed TME, tumor-infiltrating immune cells are dysfunctional, resulting in the evasion of immune surveillance and the unbridled proliferation of malignant cells [32].A series of algorithms, namely, CIBERSORT, EPIC, TIMER, XCELL, MCPCOUNTER, and QUANTISEQ, were used to assess the correlation between OLR1 expression and the level of immune cell infiltration.The results of these algorithms show that OLR1 expression is positively correlated with several cell types, particularly macrophages, myeloid dendritic cells, regulatory T cells, and cancer-associated fibroblasts, while it is negatively correlated with some specific T-cell subtypes, such as naive and Th1 CD4+ T cells (Figures 3 and S2A-C).
Int. J. Mol.Sci.2023, 24, x FOR PEER REVIEW 5 of 22 infiltrating immune cells are dysfunctional, resulting in the evasion of immune surveillance and the unbridled proliferation of malignant cells [32].A series of algorithms, namely, CIBERSORT, EPIC, TIMER, XCELL, MCPCOUNTER, and QUANTISEQ, were used to assess the correlation between OLR1 expression and the level of immune cell infiltration.The results of these algorithms show that OLR1 expression is positively correlated with several cell types, particularly macrophages, myeloid dendritic cells, regulatory T cells, and cancer-associated fibroblasts, while it is negatively correlated with some specific T-cell subtypes, such as naive and Th1 CD4+ T cells (Figures 3 and S2A-C).Immune checkpoints have emerged as a vital mechanism that is exploited by tumor cells to evade recognition and attack by T cells.ICI therapy has, to date, proven to be a clinically effective intervention for various cancers [4,5].To this end, we investigated the correlations between OLR1 and 17 common immune checkpoint expressions in pan-cancer.Notably, except for DLBC and TGCT, OLR1 exhibited positive correlations with most immune checkpoint molecules in almost all cancers, with a particularly strong correlation with HAVCR2 (Figure 4A).TMB and MSI are closely correlated with the efficacy of ICI Immune checkpoints have emerged as a vital mechanism that is exploited by tumor cells to evade recognition and attack by T cells.ICI therapy has, to date, proven to be a clinically effective intervention for various cancers [4,5].To this end, we investigated the correlations between OLR1 and 17 common immune checkpoint expressions in pan-cancer.Notably, except for DLBC and TGCT, OLR1 exhibited positive correlations with most immune checkpoint molecules in almost all cancers, with a particularly strong correlation with HAVCR2 (Figure 4A).TMB and MSI are closely correlated with the efficacy of ICI therapy [33].It was found that the expression level of OLR1 displayed strong correlations with MSI and TMB in diverse cancers (Figure 4B,C).In particular, OLR1 exhibited a positive correlation with TMB in BLCA, COAD, PRAD, SARC, and THYM, but a negative correlation in LIHC, LUAD, LUSC, and PAAD.In addition, OLR1 showed a positive correlation with MSI in COAD and READ; but conversely, it exhibited a negative correlation with MSI in HNSCC, LGG, LUSC, OV, SKCM, and STAD.Together, the expression level of OLR1 in various cancers is closely associated with the infiltration of multiple immune cells and can be used as a marker of suppressive tumor immune response, and it is expected to serve as a predictor of ICI response.
therapy [33].It was found that the expression level of OLR1 displayed strong correlations with MSI and TMB in diverse cancers (Figure 4B,C).In particular, OLR1 exhibited a positive correlation with TMB in BLCA, COAD, PRAD, SARC, and THYM, but a negative correlation in LIHC, LUAD, LUSC, and PAAD.In addition, OLR1 showed a positive correlation with MSI in COAD and READ; but conversely, it exhibited a negative correlation with MSI in HNSCC, LGG, LUSC, OV, SKCM, and STAD.Together, the expression level of OLR1 in various cancers is closely associated with the infiltration of multiple immune cells and can be used as a marker of suppressive tumor immune response, and it is expected to serve as a predictor of ICI response.

Enrichment Analysis of OLR1-Related Genes in Pan-Cancer
To further elucidate the molecular mechanism of OLR1 in tumorigenesis, we performed gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses on the top 100 proteins interacting with OLR1 obtained from the STRING database (Table S3 and Figure S4).The GO analysis was divided into three parts as follows: The biological processes (BP) enriched in this dataset were mainly related to "response to molecule of bacterial", "response to lipopolysaccharide", "regulation of inflammatory" and "regulation of cell-cell adhesion"; the cellular components (CC) enriched were related to "external side of plasma membrane", "endocytic vesicle", and "tertiary granule membrane"; and the enriched molecular functions (MF) were linked to "lipoprotein particle binding", "cargo receptor activity", and "scavenger receptor activity" (Figure 5A).According to the KEGG analysis, we unexpectedly observed that OLR1 was closely associated with "the PD-L1 expression and PD-1 checkpoint pathway in cancer", "the NOD-like receptor signaling pathway", "TNF signaling pathway", and "the Toll-like receptor signaling pathway" (Figure 5B), suggesting that OLR1 may play a role in tumor immune regulation.We also found a close link between OLR1 and HIF-1 as well as the NF-κB signaling pathway, suggesting that OLR1 may play an important role in the processes of tumorigenesis and metastasis.In addition, the GO and KEGG analyses both suggested that OLR1 was associated with intercellular adhesion, suggesting that it may be involved in regulating cell migration.Furthermore, we constructed a protein-protein interaction (PPI) network for OLR1 and the top 10 associated genes using STRING (Figure 5C).Additionally, we found that most of these genes were reported to be associated with tumor progression.We also investigated whether OLR1 affected antitumor drug sensitivity using the CellMiner dataset [34] and found that OLR1 correlated with resistance to drugs, such as vincristine and paclitaxel (Figure 5D, Table S4).These results suggest that OLR1 expression may impact the efficacy of chemotherapy in certain cancers.
related to "response to molecule of bacterial", "response to lipopolysaccharide", "regulation of inflammatory" and "regulation of cell-cell adhesion"; the cellular components (CC) enriched were related to "external side of plasma membrane", "endocytic vesicle", and "tertiary granule membrane"; and the enriched molecular functions (MF) were linked to "lipoprotein particle binding", "cargo receptor activity", and "scavenger receptor activity" (Figure 5A).According to the KEGG analysis, we unexpectedly observed that OLR1 was closely associated with "the PD-L1 expression and PD-1 checkpoint pathway in cancer", "the NOD-like receptor signaling pathway", "TNF signaling pathway", and "the Toll-like receptor signaling pathway" (Figure 5B), suggesting that OLR1 may play a role in tumor immune regulation.We also found a close link between OLR1 and HIF-1 as well as the NF-κB signaling pathway, suggesting that OLR1 may play an important role in the processes of tumorigenesis and metastasis.In addition, the GO and KEGG analyses both suggested that OLR1 was associated with intercellular adhesion, suggesting that it may be involved in regulating cell migration.Furthermore, we constructed a protein-protein interaction (PPI) network for OLR1 and the top 10 associated genes using STRING (Figure 5C).Additionally, we found that most of these genes were reported to be associated with tumor progression.We also investigated whether OLR1 affected antitumor drug sensitivity using the CellMiner dataset [34] and found that OLR1 correlated with resistance to drugs, such as vincristine and paclitaxel (Figure 5D, Table S4).These results suggest that OLR1 expression may impact the efficacy of chemotherapy in certain cancers.

OLR1 Overexpression in HNSCC and Resistance to Immunotherapy
The abovementioned results indicate that OLR1 may play an important role in HNSCC.Considering the fact that immunotherapy exhibits a response rate of less than 20% in HNSCC patients, there is an urgent need to develop novel targets or strategies to enhance the efficacy of immunotherapy [35].Therefore, as a follow-up to our pan-cancer analysis, we further investigated the underlying mechanisms by which OLR1 influences HNSCC progression, the sixth most common cancer worldwide [1].First, we observed a significant increase in OLR1 expression levels in tumor tissues compared to the normal controls in the TCGA-HNSCC dataset (p < 0.001, Figure 6A,B).Then, we analyzed the correlation between OLR1 expression and clinical features, demonstrating that HNSCC patients with higher T stage (TNM classification) had higher OLR1 expression levels than those with lower T stage (T1 vs. T3 and T4, p < 0.05, Figure 6C).Additionally, OLR1 expression was positively correlated with a higher pathological grade (I vs. II, p < 0.01; I vs. III and IV, p < 0.01, Figure 6D).Then, we performed IHC staining and confirmed that LOX-1 was overexpressed in both human and mouse HNSCC (p < 0.05 and p < 0.01, respectively, Figure 6E,F).Notably, both the univariate and multifactorial Cox regression analyses indicated that OLR1 could serve as an independent prognostic factor for OS in HNSCC patients (p = 0.004 and p = 0.013, respectively, Figure 6G).Furthermore, the Kaplan-Meier survival analysis using the log-rank test in the GSE41613 datasets reinforced our findings, suggesting that HNSCC patients with elevated OLR1 expression levels presented worse outcomes (p = 0.01, Figure 6H).Therefore, these results suggest that OLR1 overexpression is closely associated with aggressive cancer behavior and poor prognosis in HNSCC.Then, we used the tumor immune dysfunction and exclusion (TIDE) algorithm to evaluate the potential clinical efficacy of immunotherapy in different OLR1-expressing Then, we used the tumor immune dysfunction and exclusion (TIDE) algorithm to evaluate the potential clinical efficacy of immunotherapy in different OLR1-expressing subgroups.Our results reveal that OLR1 expression is significantly reduced in HNSCC patients exhibiting a high efficacy of immunotherapy responses compared to those who are immunotherapy resistant (Figure 6I).Additionally, the TIDE scores were markedly elevated in the OLR1 high-expression subgroup in contrast to the OLR1 low-expression subgroup, implying that HNSCC patients with higher OLR1 expression levels were more likely to be resistant to ICI therapy than those with lower OLR1 expression levels (p < 0.001, Figure 6J).Moreover, we found that the MSI score was higher in the OLR1 low-expression subgroup (p < 0.001, Figure 6K), while the T-cell exclusion and T-cell dysfunction scores were higher in the OLR1 high-expression subgroup (p < 0.05 and p < 0.001, respectively, Figure 6L,M).These results suggest that OLR1 expression is associated with cancer immune escape and targeting OLR1 may increase tumor T-cell infiltration as well as MSI, thereby improving the response rate to ICI therapy.

OLR1 Knockdown Inhibits the EMT Process and Stem Cell Properties via the STAT3 Pathway in HNSCC Cells
Given the association between OLR1 expression and clinical outcomes in HNSCC, we subsequently sought to explore the potential mechanisms by which OLR1 contributes to HNSCC progression.A gene set enrichment analysis (GSEA) of OLR1 was performed using the TCGA-HNSCC dataset and we found that OLR1 may be involved in multiple oncogenic signaling pathways; notably, there was a strong positive correlation between OLR1 and the EMT process (Figure 7A).In addition, OLR1 showed a significant positive correlation with mesenchymal markers (FN1, VIM, and MMP9) and EMT-TFs (SNAI1 and ZEB1, Figure 7B).The EMT process has been widely demonstrated to be associated with invasion, metastasis, immunosuppression, and therapy resistance in a variety of tumors [8].Thus, to further verify whether OLR1 affected the EMT process in HNSCC, we utilized siRNA to reduce OLR1 expression in the HNSCC cell lines Cal27 and SCC4.The Western blotting results show that the "mesenchymal" characteristics (N-cadherin and Vimentin) of Cal27 and SCC4 diminish following OLR1 deletion, while the "epithelial" characteristic (E-cadherin) is significantly enhanced (Figure 7C).This result suggests that OLR1 silencing restricts epithelial-mesenchymal plasticity in HNSCC.Subsequently, through the cell scratch assay, we found that the healing ability of OLR1 knockdown cells was significantly inhibited (p < 0.001, Figure 7D).Moreover, we used the Transwell experiment to verify that the invasive ability of Cal27 cells was markedly suppressed following the OLR1 knockdown (p < 0.01, Figure 7E).
Next, we investigated the regulatory mechanism of OLR1 in HNSCC.The results of the GSEA analysis suggest that OLR1 is associated with STAT3 signaling (Figure 7A), which is an important signaling pathway for invasion and the maintenance of stemness properties [36].Consistent with this result, we observed that OLR1 knockdown significantly reduced STAT3 activation by tyrosine phosphorylation and the expression of stemness marker (CD44) (Figure 7F).Furthermore, our study also showed that the knockdown of OLR1 inhibited the proliferative capacity and sphere-forming ability of HNSCC cells (p < 0.01 and p < 0.05, respectively, Figure 8A,B).Furthermore, the ALDEFLUOR assay revealed that the proportion of ALDH high cells was decreased in the siOLR1 group, suggesting the critical role of OLR1 in regulating stemness properties in HNSCC (p < 0.01 and p < 0.05, respectively, Figure 8C,D).Taken together, these data suggest roles for OLR1 in the maintenance of the EMT process and stemness characteristics through the STAT3 pathway in HNSCC.Next, we investigated the regulatory mechanism of OLR1 in HNSCC.The results of the GSEA analysis suggest that OLR1 is associated with STAT3 signaling (Figure 7A), which is an important signaling pathway for invasion and the maintenance of stemness properties [36].Consistent with this result, we observed that OLR1 knockdown significantly reduced STAT3 activation by tyrosine phosphorylation and the expression of stemness marker (CD44) (Figure 7F).Furthermore, our study also showed that the knockdown of OLR1 inhibited the proliferative capacity and sphere-forming ability of HNSCC cells (p < 0.01 and p < 0.05, respectively, Figure 8A,B).Furthermore, the ALDEFLUOR assay revealed that the proportion of ALDH high cells was decreased in the siOLR1 group, suggesting the critical role of OLR1 in regulating stemness properties in HNSCC (p < 0.01 and p < 0.05, respectively, Figure 8C,D).Taken together, these data suggest roles for OLR1 in the maintenance of the EMT process and stemness characteristics through the STAT3 pathway in HNSCC.

OLR1 Acts as a Repressor of Cuproptosis in HNSCC Cells
Cuproptosis, which is a unique mode of cell death, is highly correlated with cellular metabolism [11].Cells dependent on glycolysis tend to be more resistant to cuproptosis than cells dependent on mitochondrial respiration, suggesting that targeting glycolysis may be effective in enhancing the efficacy of cuproptosis [37].Considering that OLR1 can drive glycolytic metabolism [21], we decided to further explore the relationship between OLR1 and cuproptosis sensitivity.Therefore, we subsequently integrated the TCGA-HNSCC database and observed correlations between OLR1 and known cuproptosis-related genes [10] (FDX1, DLAT, ATP7A, ATP7B, CDKN2A, and GLS) (Figure 9A).Therefore, we decided to explore the relationship between OLR1 and cuproptosis.Elesclomol acts as a copper ionophore and exerts antitumor effects by effectively inducing the onset of cuproptosis [38].In our study, we found that elesclomol-Cu promoted the death of HNSCC cells in a concentration-dependent manner (Figure 9B).Importantly, the knockdown of OLR1 significantly enhanced elesclomol-Cu-induced cell death (Figure 9B).This finding was further verified using the annexin V/PI assay.In addition, tetrathiomolybdate (TTM) prevented cell death (p < 0.001, Figure 9C,D).In the process of cuproptosis, copper binds to lipoylated DLAT and induces the oligomerization of DLAT.The increase in insoluble DLAT leads to cytotoxicity and induces cuproptosis [10].We found that, following elesclomol-Cu treatment, the OLR1 silencing group presented a more pronounced DLAT

OLR1 Acts as a Repressor of Cuproptosis in HNSCC Cells
Cuproptosis, which is a unique mode of cell death, is highly correlated with cellular metabolism [11].Cells dependent on glycolysis tend to be more resistant to cuproptosis than cells dependent on mitochondrial respiration, suggesting that targeting glycolysis may be effective in enhancing the efficacy of cuproptosis [37].Considering that OLR1 can drive glycolytic metabolism [21], we decided to further explore the relationship between OLR1 and cuproptosis sensitivity.Therefore, we subsequently integrated the TCGA-HNSCC database and observed correlations between OLR1 and known cuproptosis-related genes [10] (FDX1, DLAT, ATP7A, ATP7B, CDKN2A, and GLS) (Figure 9A).Therefore, we decided to explore the relationship between OLR1 and cuproptosis.Elesclomol acts as a copper ionophore and exerts antitumor effects by effectively inducing the onset of cuproptosis [38].In our study, we found that elesclomol-Cu promoted the death of HNSCC cells in a concentration-dependent manner (Figure 9B).Importantly, the knockdown of OLR1 significantly enhanced elesclomol-Cu-induced cell death (Figure 9B).This finding was further verified using the annexin V/PI assay.In addition, tetrathiomolybdate (TTM) prevented cell death (p < 0.001, Figure 9C,D).In the process of cuproptosis, copper binds to lipoylated DLAT and induces the oligomerization of DLAT.The increase in insoluble DLAT leads to cytotoxicity and induces cuproptosis [10].We found that, following elesclomol-Cu treatment, the OLR1 silencing group presented a more pronounced DLAT oligomerization compared with the control group (Figure 9C,D).Together, these results suggest that OLR1 may inhibit the occurrence of cuproptosis by inhibiting the oligomerization of DLAT.In summary, OLR1 is associated with poor prognosis and may serve as an emerging target for cancer therapy.

Discussion
The role of OLR1 in tumorigenesis has only recently come to light, with previous studies primarily focusing on cardiovascular and metabolic diseases.Recent reports in the field have identified its overexpression in diverse malignancies, including breast [18,19],

Discussion
The role of OLR1 in tumorigenesis has only recently come to light, with previous studies primarily focusing on cardiovascular and metabolic diseases.Recent reports in the field have identified its overexpression in diverse malignancies, including breast [18,19], colon [21], esophageal [22,23], and pancreatic cancers [20,25].These results are consistent with our results.We validated this outcome in HNSCC and found that OLR1 expression was significantly higher in HNSCC and correlated with advanced clinicopathological factors in the TCGA dataset.In addition, OLR1 has been closely correlated with clinical outcomes in a few cancer types [19,20,25], which was also observed in our results.Specifically, both univariate and multifactorial survival analyses indicated that OLR1 was a poor prognostic indicator for HNSCC patients.These results suggest that OLR1 may be a valuable prognostic biomarker across different cancer types.
The TME consists of diverse immune cell types that can play a crucial role in either tumor cell clearance or tumor immune escape [39,40].Previous studies have revealed that the degree of immune cell infiltration in the TME is related to tumor development and prognosis [41].This study determined that OLR1 expression was positively correlated with several cell types in a variety of tumors, particularly macrophages, myeloid dendritic cells, regulatory T cells, and cancer-associated fibroblasts, which play major roles in promoting angiogenesis, immunosuppression, metastasis formation, and therapy-resistance behaviors.There is evidence that the aberrant activation of OLR1 in PMN-MDSCs is associated with tumor immunosuppression [27,28].Therefore, we preliminarily speculated that OLR1 might promote tumor development by recruiting these immunosuppressive cells to infiltrate the TME.
In recent years, immunotherapies based on ICI have developed rapidly.They have achieved impressive results in the treatment of several types of cancer.However, only a small percentage of patients have experienced clinical benefits [4].Therefore, there is a need for the development of biomarkers that can predict whether patients will benefit from these therapies.Immune checkpoints, such as CTLA-4, PD-1, and TIM-3, play important roles in regulating T-cell responses and have been shown to be effective targets for cancer therapy [42,43].The expression of constitutive co-suppressor receptors on T cells inhibits effector T-cell responses.Notably, our correlation analysis results showed that OLR1 was positively correlated with most immune checkpoint molecules in the majority of cancer types, with a particularly strong correlation with HAVCR2.The KEGG analysis also revealed a significant correlation between OLR1 and the PD-1/PD-L1 pathway.To further investigate the relationship between OLR1 and immunotherapy, we correlated OLR1 expression with TMB and MSI, which were evaluated as potential biomarkers for predicting ICI therapy responses [33].Additionally, we found that OLR1 was correlated with both TMB and MSI in specific cancer types.Moreover, TIDE integrates expression signatures of T-cell dysfunction and T-cell exclusion to mimic tumor immune evasion, and thus predict clinical responses to ICI [44].An elevated TIDE score correlates with an increased tendency for immune evasion and a reduced response to ICI therapy.Consequently, the TIDE algorithm was used to validate the relevance of OLR1 to immunotherapy.Interestingly, the results show that HNSCC patients with high OLR1 expression levels have higher TIDE, T-cell exclusion, and dysfunction scores, but lower MSI scores.These results suggest that patients with high OLR1 expression levels may be resistant to treatment with ICI.Taken together, OLR1 may serve as a potential predictor for immunotherapy.
EMT is an important process of normal embryonic development and tissue regeneration processes [45].However, abnormal reactivation of the EMT process during cancer progression and metastasis is associated with malignant properties of tumor cells, including promoting migration and aggressiveness, as well as increasing tumor stemness [8].Recent studies have revealed that the EMT process can modulate the tumor microenvironment and facilitate immune escape, resulting in immunotherapy resistance [46].OLR1 has been demonstrated to participate in modulation of the EMT process, thereby instigating the metastasis of pancreatic cancer [20,25] and osteosarcomas [47].In line with these findings, we observed a robust positive correlation between OLR1 and common EMT-related genes in pan-cancer (Figure S3).Moreover, the GSEA results showed that OLR1 exhibited the strongest correlation with the EMT hallmark in HNSCC.The correlation analysis results further verified that OLR1 was positively correlated with mesenchymal markers and EMT-TFs.Thus, to investigate whether OLR1 affected the EMT phenotype, we knocked down the expression of LOX-1 in vitro.The Western blotting results showed that the mesenchymal properties of HNSCC cells weakened after OLR1 depletion.Additionally, the knockdown of OLR1 significantly inhibited the invasive ability of HNSCC cells.The EMT process was closely related to stem cell-like properties, and we also found suppressed stemness and proliferation abilities in the siOLR1 group.The STAT3 signaling pathway was implicated in the regulation of proliferation, survival, invasion, stem cells, and immunosuppression [36].Furthermore, its interaction with the EMT process has been identified as a crucial regulator of tumor metastasis [48].Notably, our study revealed that inhibiting OLR1 impeded activation of the STAT3 pathway.These results suggest that OLR1 may play a pivotal role in modulating the EMT process via the STAT3 pathway in HNSCC.
Copper nanoparticle-based therapeutics have demonstrated the ability to induce cuproptosis in cancer cells, thereby notably enhancing dendritic cell maturation and promoting infiltration by antitumor CD8+ T cells [49].An increasing number of researchers, by employing bioinformatics analyses, have demonstrated that cuproptosis was implicated in tumorigenesis, further contributing to the intricate complexities of tumor immune evasion [50].This evidence suggests a potential association of cuproptosis with immunogenic phenotypes.Exploring cuproptosis holds the potential to improve our understanding of tumorigenesis and tumor microenvironment remodeling behaviors.At present, limited research exists regarding a correlation between HNSCC and cuproptosis, with only one bioinformatics study indicating a potential correlation between cuproptosis and prognosis, as well as immune responses in HNSCC [51].Considering the important role of OLR1 in driving tumor glycolysis and its relevance to the immunosuppressive tumor microenvironment and tumor metastasis, we further investigated the relationship between OLR1 and cuproptosis.Our results showed that OLR1 silencing significantly enhanced elesclomol-induced cuproptosis.Copper directly binds to DLAT, an enzyme involved in the regulation of the mitochondrial tricarboxylic acid (TCA) cycle, promoting the disulfide-bond-dependent aggregation of lipoylated DLAT, leading to cellular cuproptosis.Consistent with this notion, OLR1 knockdown enhanced DLAT oligomerization following elesclomol treatment.These results suggest that OLR1 may negatively regulate elesclomol by regulating the oligomerization of DLAT.
Our study results revealed the potential significance of OLR1 in tumor immunity and prognosis; however, there are still some limitations worth considering.Most of the conclusions of this study were based on bioinformatics analysis, and while we conducted in vitro experiments to support our hypothesis, further in vivo experiments on mice would provide a more complete understanding of the immunomodulatory effects of OLR1.Our results suggest a potential association between OLR1 and pro-immunogenic tumor phenotypes; however, further mechanistic studies are required to elucidate the underlying molecular pathways, as well as clinical validations to determine its utility in guiding treatment decisions.Another limitation of this study was the relatively small sample size of the clinical cohort that we used, which may have affected the statistical power of our analysis.Despite these limitations, our study sets the stage for future research in this area and provides valuable insights into the potential role of OLR1 in tumor therapy.

Bioinformatics Data and Resources
RNA-seq data for 546 HNSCC cases including 502 HNSCC tumor samples and 44 normal tissue samples, were obtained from The Cancer Genome Atlas (TCGA) database (https://portal.gdc.cancer.gov/,accessed on 7 March 2023).RNA expression data and survival information for 97 HNSCC samples (GSE41613) were obtained from the Gene Expression Omnibus (GEO; https://www.ncbi.nlm.nih.gov/geo/,accessed on 10 March 2023) [52].All raw data are provided in the Supplementary Materials.
In order to assess OLR1 expression patterns across various cancer types, we retrieved RNA-Seq data from the TCGA database.The TCGA data portal was searched to obtain transcripts per million (TPM) values for OLR1 in different cancer types.The TPM value is a normalization method used for RNA-seq, which considers both sequencing depth and gene length.In our study, we focused on tumor samples and utilized Log2(TPM+1) values.A differential expression analysis was performed using t-tests or ANOVA statistical tests based on the distribution of the data.The significance threshold was set at p < 0.05 after correction for multiple testing.To visualize the expression pattern, boxplots and heatmaps were generated using R-based data visualization packages.

Survival Analysis
Kaplan-Meier and Cox regression analyses were conducted to explore the influence of OLR1 on patient prognosis using the R packages "survminer" and "survival".Overall survival (OS), progression-free interval (PFI), and disease-specific survival (DSS) values were also evaluated.The hazard ratio (HR) for deaths associated with OLR1 expression was estimated by using the univariate Cox proportional hazards regression model.Then, a multivariate Cox model was constructed to estimate the adjusted HR for OLR1 expression; p-values less than 0.05 were considered to be statistically significant.

PPI, GO, and KEGG Analyses
The protein-protein interaction (PPI) network and enrichment analysis and the STRING (https://string-db.org/,accessed on 8 August 2023) database were used to construct the PPI of OLR1.The minimum required interaction score was set at 0.4.The gene oncology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of OLR1-related molecules were performed using clusterProfiler packages.

Immune Landscape Analysis
The TIMER2.0 database (http://timer.comp-genomics.org/,accessed on 27 March 2023) provided 6 different immune infiltrating cell algorithms: CIBERSORT, EPIC, TIMER, XCELL, MCPCOUNTER, and QUANTISEQ [53].The scoring data of the 6 tumor-infiltrating immune cells were downloaded from the TIMER2.0database.Spearman's correlation test was used to investigate the relationship between OLR1 expression and the 6 types of immune cells in pan-cancer.In addition, the TIMER2.0database was used to assess the correlations between immune checkpoint-related and EMT-related gene expressions and OLR1 expression.
To predict the immunotherapy response in different OLR1 subgroups, tumor immune dysfunction and exclusion (TIDE) scores for 502 HNSCC samples were calculated using an algorithm provided by Jiang et al. [44].The TIDE score evaluated two distinct tumor immune evasion mechanisms: the dysfunction of tumor-infiltrating cytotoxic T lymphocytes (CTLs) and the exclusion of CTLs by immunosuppressive factors.
Level 4 simple nucleoside variation data processed with MuTect2 in GATK V4.1.8.1 software (Broad Institute, Cambridge, MA, USA) were acquired from the TCGA database, and the TMB was calculated [54].The microsatellite instability signatures for 33 tumors were obtained from reported studies [55].Correlations between OLR1 mRNA expression and MSI or TMB were investigated by Spearman's correlation test.

Gene Set Enrichment Analysis (GSEA)
For the signaling pathway analysis, the HNSCC patients were initially divided into two subgroups based on the median expression levels of OLR1.Then, a differential expression analysis was performed between the high-and low-expression subgroups.The input genes of GSEA were sorted by their log fold change (logFC) values.Then, an enrichment analysis was performed using the GSEA method with the cluster Profiler package of R to identify the signaling pathways in which the differentially expressed genes were involved [56].

HNSCC Samples
The human tissue samples analyzed in our study included 6 primary HNSCC and 5 normal oral mucosae collected from the School and Hospital of Stomatology, Wuhan University, Department of Oral Maxillofacial-Head Neck Oncology, in 2022, with informed consent obtained from the patients.All human tissue samples were collected with reference to our previous studies [57].Age, sex, tumor grading, and TNM staging were obtained from patient medical records (Table S1).The 4-nitroquinoline-1-oxide (4NQO)-induced HNSCC specimens used in this study were collected and preserved as paraffin-embedded blocks in our previous study [58].

Immunohistochemistry
Immunohistochemical staining was conducted as previously described [59].Briefly, human and mouse HNSCC samples were paraffin embedded and sectioned at 4 µm.Following xylene deparaffinization and gradient ethanol soaking rehydration, the sections were subjected to high-temperature antigen retrieval with a citrate antigen retrieval solution and endogenous peroxidase inhibition with 3% hydrogen peroxide.Then, the non-specific binding was blocked using 5% goat serum (Mxb Biotechnologies, Fuzhou, China, KIT-9710).The processed sections were then incubated with primary antibodies (Table S2) at 4 • C overnight, washed with PBS, and incubated with horseradish peroxidase-conjugated secondary antibodies (Mxb Biotechnologies, KIT-9710) at 37 • C for 30 min.A DAB substrate kit (Mxb Biotechnologies, DAB-0031) and hematoxylin were used for staining.Images were visualized using a Pannoramic MIDI scanner (3DHISTECH) and quantified using the Aperio ScanScope software (v11.1.2.752, Aperio, San Diego, CA, USA).IgG was used as a negative control.

Cell Lines
The Cal27 and SCC4 cells were obtained from the China Center for Type Culture Collection (Shanghai, China) and were grown for no more than 15 generations.The Cal27 cells were cultured in high glucose DMEM, while the SCC4 cells were cultured in F12/DMEM.Mycoplasma contamination assays were performed annually by PCR.

Western Blotting
The experiments were performed as previously described [59].The extraction of total protein was performed with RIPA buffer supplemented with protease and phosphatase inhibitors (Roche, P7626; P2850).A BCA protein assay kit (Beyotime, Shanghai, China, P0012S) was used to determine the protein concentrations.The protein samples were separated on a 10% SDS-PAGE gel and transferred onto a polyvinylidene difluoride membrane (Roche, Basel, Switzerland, 3010040001).Then, the membrane was blocked in 5% nonfat milk for 1 h at room temperature, incubated with primary antibodies (Table S2) overnight at 4 • C, washed with TBST, and incubated with horseradish peroxidase-conjugated secondary antibodies (Proteintech, Rosemont, IL, USA, SA00001-1/2) for 1 h at room temperature.An ECL-enhanced chemiluminescent substrate kit (Advansta, K-12045-D50) and Odyssey system (Li-Cor Biosciences, Lincoln, NE, USA) were used for visualization and quantification.

Transwell Invasion Assay
The 24-well Transwell chambers (8 um pore, Corning, Corning, NY, USA) were prepared overnight with 60 µL matrix gel (BD Biosciences, NJ, USA, 256234).The transfected HNSCC cells (5 × 10 4 cells) were inoculated in the upper chamber with 200 µL of serumfree DMEM, and 600 µL of DMEM containing 10% FBS was added to the lower chamber.After 48 h of incubation at 37 • C, the cells were fixed with 4% paraformaldehyde and stained with crystal violet.The uninvaded upper cells were erased.Cell invasion was captured under an inverted microscope.

Wound Healing Assay
The transfected HNSCC cells were inoculated in 6-well plates (1 × 10 6 cells); when the density reached approximately 95%, the cells were scratched with a 200 µL pipette tip.After cleaning the cell surface with PBS, the medium was replaced with fresh serum-free medium.The healing dynamics of the affected area were subsequently monitored using high-powered microscopic visualization at 0-and 24-hour intervals.

Cell Proliferation
The experiments were performed according to the protocol of our previous study [59].Briefly, the proliferation rates of the Cal27 and SCC4 cells were measured using the CCK-8 assay (Dojindo, Japan).HNSCC cells at a density of 2000 cells per well in the corresponding medium were inoculated into 96-well plates for the CCK-8 assay.Then, the absorbance was measured at 450 nm using a BioTek plate reader (BioTek, Winooski, VT, USA).

Flow Cytometry
To detect cell stemness, an ALDEFLUOR TM kit (STEMCELL Technologies, Vancouver, BC, Canada) was used to detect aldehyde dehydrogenase (ALDH) activity as previously described [59].Briefly, OLR1 knockdown and control cells were incubated with ALDEFLUOR reagent, and diethylaminobenzaldehyde (DEAB) was used as a negative control.For the cell death detection assay, cells were digested with EDTA-free trypsin and washed with PBS buffer followed by detection with an Annexin V-FITC/PI apoptosis kit (EBioscience, San Diego, CA, USA).The data were acquired using CytoFLEX S (Beckman Coulter, Brea, CA, USA) and analyzed by the FlowJo software (V7.6.2,Treestar, Woodburn, OR, USA).PI-positive cells were counted to assess the occurrence of cuproptosis.

Statistical Analysis
All statistical analyses were performed using the GraphPad Prism 8.0 and R 3.6.3software.Two-tailed Student's t-test was used to perform comparisons between two groups, and one-way ANOVA and Tukey's multiple comparison tests were used for multiple comparisons.A Spearman's correlation analysis was used for the correlation analysis.TIDE scores were compared using the Wilcoxon test.All results were calculated in at least three independent experiments.All error bar values represent standard deviations (SD);

Figure 2 .
Figure 2. Prognostic analysis of OLR1 in different cancer types: (A) Forest plot showing the correlation between OLR1 expression and OS in various cancers; (B-K) Kaplan-Meier survival analysis results show that overall survival is decreased in CESC, COAD, HNSCC, LGG, SARC, STAD, and THYM patients with higher OLR1 expression levels, whereas the opposite result is observed in CHOL, LUAD, and SKCM patients.

Figure 2 .
Figure 2. Prognostic analysis of OLR1 in different cancer types: (A) Forest plot showing the correlation between OLR1 expression and OS in various cancers; (B-K) Kaplan-Meier survival analysis resultsshow that overall survival is decreased in CESC, COAD, HNSCC, LGG, SARC, STAD, and THYM patients with higher OLR1 expression levels, whereas the opposite result is observed in CHOL, LUAD, and SKCM patients.

Figure 3 .
Figure 3. Correlation between OLR1 expression and immune cell infiltration in various pan-cancer types using: (A) The CIBERSORT algorithm; (B) the EPIC algorithm; (C) the TIMER algorithm.

Figure 3 .
Figure 3. Correlation between OLR1 expression and immune cell infiltration in various pan-cancer types using: (A) The CIBERSORT algorithm; (B) the EPIC algorithm; (C) the TIMER algorithm.

Figure 4 .
Figure 4. Overexpressed OLR1 is positively correlated with immune checkpoint molecules in multiple cancers: (A) Heatmap showing correlations between OLR1 expression and 17 common immune checkpoint genes' RNA levels in the TCGA pan-cancer database by the Spearman's correlation test; (B) radar plot showing the correlation between OLR1 expression and TMB in different cancers; (C) radar plot showing the correlation between OLR1 expression and MSI in different cancers.* p < 0.05, ** p < 0.01, and *** p < 0.001.

Figure 4 .
Figure 4. Overexpressed OLR1 is positively correlated with immune checkpoint molecules in multiple cancers: (A) Heatmap showing correlations between OLR1 expression and 17 common immune checkpoint genes' RNA levels in the TCGA pan-cancer database by the Spearman's correlation test; (B) radar plot showing the correlation between OLR1 expression and TMB in different cancers; (C) radar plot showing the correlation between OLR1 expression and MSI in different cancers.* p < 0.05, ** p < 0.01, and *** p < 0.001.

Figure 5 .
Figure 5. Functional enrichment analysis of OLR1-related genes: (A) GO and (B) KEGG enrichment analyses of the top 100 OLR1-related genes; (C) protein-protein interaction network for OLR1 using the STRING datase; (D) drug sensitivity analysis of OLR1 using the CellMiner database.Dashed line indicates p < 0.05.

Figure 5 .
Figure 5. Functional enrichment analysis of OLR1-related genes: (A) GO and (B) KEGG enrichment analyses of the top 100 OLR1-related genes; (C) protein-protein interaction network for OLR1 using the STRING datase; (D) drug sensitivity analysis of OLR1 using the CellMiner database.Dashed line indicates p < 0.05.

Figure 7 .
Figure 7. Effect of OLR1 knockdown on the EMT process in HNSCC cell lines: (A) GSEA results of OLR1 based on the TCGA-HNSCC dataset; (B) Volcano plot of OLR1-associated genes in the TCGA HNSCC cohort by the Pearson's correlation coefficient test; (C) Western blot analysis of LOX-1, Ncadherin, E-cadherin, and vimentin expressions in OLR1 knockdown or control Cal27 and SCC4 cells; (D) representative images and quantitative analyses of wound healing (20×) and (E) Transwell assay (40×) in Cal27 cells (** p < 0.01 and *** p < 0.001, the red dash indicates the edge of the scratch); (F) expression of STAT3, p-STAT3, and CD44 were analyzed by Western blot.

Figure 7 .
Figure 7. Effect of OLR1 knockdown on the EMT process in HNSCC cell lines: (A) GSEA results of OLR1 based on the TCGA-HNSCC dataset; (B) Volcano plot of OLR1-associated genes in the TCGA HNSCC cohort by the Pearson's correlation coefficient test; (C) Western blot analysis of LOX-1, N-cadherin, E-cadherin, and vimentin expressions in OLR1 knockdown or control Cal27 and SCC4 cells; (D) representative images and quantitative analyses of wound healing (20×) and (E) Transwell assay (40×) in Cal27 cells (** p < 0.01 and *** p < 0.001, the red dash indicates the edge of the scratch); (F) expression of STAT3, p-STAT3, and CD44 were analyzed by Western blot.

Figure 8 .
Figure 8. OLR1 participates in the maintenance of stemness characteristics in HNSCC cells: (A) Effect of OLR1 knockdown on Cal27 and SCC4 cell growth as determined by CCK-8 (* p < 0.05 and ** p < 0.01); (B) the effect of OLR1 knockdown on the stemness properties of Cal27 cells was evaluated using the sphere formation assay (* p < 0.05 and bar, 100 µm), data are mean ± s.d.; (C,D) ALDH activity in OLR1-deficient and control cells was quantified by flow cytometry, *** p < 0.001.

Figure 8 .
Figure 8. OLR1 participates in the maintenance of stemness characteristics in HNSCC cells: (A) Effect of OLR1 knockdown on Cal27 and SCC4 cell growth as determined by CCK-8 (* p < 0.05 and ** p < 0.01); (B) the effect of OLR1 knockdown on the stemness properties of Cal27 cells was evaluated using the sphere formation assay (* p < 0.05 and bar, 100 µm), data are mean ± s.d.; (C,D) ALDH activity in OLR1-deficient and control cells was quantified by flow cytometry, *** p < 0.001.

Figure 9 .
Figure 9. OLR1 acts as a repressor of cuproptosis in HNSCC cells: (A) Correlations of OLR1 and cuproptosis-related genes in the TCGA-HNSCC dataset; (B) cell viability of Cal27 and SCC4 cells following treatment with the indicated concentrations of elesclomol (* p < 0.05); (C) cuproptosis cells were stained with Annexin V and PI for flow cytometry in Cal27 treated with elesclomol (40 nM) and TTM (20 nM); (D) quantitative statistical analysis shows different percentages of cell death in siOLR1 and the control group (*** p < 0.001, ns, no significant difference); (E) DLAT oligomerization was analyzed by Western blot in Cal27 cells that were treated with elesclomol (40 nM); (F) schematic illustration of the role of OLR1 in HNSCC.In brief, the elevated expression of OLR1 activates the STAT3 signaling pathway to regulate metastasis, the EMT process, and stemness characteristics in HNSCC.In addition, OLR1 negatively modulates cuproptosis by inhibiting DLAT oligomerization.In summary, OLR1 is associated with poor prognosis and may serve as an emerging target for cancer therapy.

Figure 9 .
Figure 9. OLR1 acts as a repressor of cuproptosis in HNSCC cells: (A) Correlations of OLR1 and cuproptosis-related genes in the TCGA-HNSCC dataset; (B) cell viability of Cal27 and SCC4 cells following treatment with the indicated concentrations of elesclomol (* p < 0.05); (C) cuproptosis cells were stained with Annexin V and PI for flow cytometry in Cal27 treated with elesclomol (40 nM) and TTM (20 nM); (D) quantitative statistical analysis shows different percentages of cell death in siOLR1 and the control group (*** p < 0.001, ns, no significant difference); (E) DLAT oligomerization was analyzed by Western blot in Cal27 cells that were treated with elesclomol (40 nM); (F) schematic illustration of the role of OLR1 in HNSCC.In brief, the elevated expression of OLR1 activates the STAT3 signaling pathway to regulate metastasis, the EMT process, and stemness characteristics in HNSCC.In addition, OLR1 negatively modulates cuproptosis by inhibiting DLAT oligomerization.In summary, OLR1 is associated with poor prognosis and may serve as an emerging target for cancer therapy.