Expression Profiling along the Murine Intestine: Different Mucosal Protection Systems and Alterations in Tff1-Deficient Animals

Tff1 is a typical gastric peptide secreted together with the mucin, Muc5ac. Tff1-deficient (Tff1KO) mice are well known for their prominent gastric phenotype and represent a recognized model for antral tumorigenesis. Notably, intestinal abnormalities have also been reported in the past in these animals. Here, we have compared the expression of selected genes in Tff1KO mice and their corresponding wild-type littermates (RT-PCR analyses), focusing on different mucosal protection systems along the murine intestine. As hallmarks, genes were identified with maximum expression in the proximal colon and/or the duodenum: Agr2, Muc6/A4gnt/Tff2, Tff1, Fut2, Gkn2, Gkn3, Duox2/Lpo, Nox1. This is indicative of different protection systems such as Tff2/Muc6, Tff1-Fcgbp, gastrokines, fucosylation, and reactive oxygen species (ROS) in the proximal colon and/or duodenum. Few significant transcriptional changes were observed in the intestine of Tff1KO mice when compared with wild-type littermates, Clca1 (Gob5), Gkn1, Gkn2, Nox1, Tff2. We also analyzed the expression of Tff1, Tff2, and Tff3 in the pancreas, liver, and lung of Tff1KO and wild-type animals, indicating a cross-regulation of Tff gene expression. Furthermore, on the protein level, heteromeric Tff1-Fcgbp and various monomeric Tff1 forms were identified in the duodenum and a high-molecular-mass Tff2/Muc6 complex was identified in the proximal colon (FPLC, proteomics).


Introduction
The intestinal tract consists of two major segments, i.e., the small intestine (duodenum, jejunum, and ileum) and the large intestine (caecum, colon, and rectum), which differ in their morphology [1].The lumen is lined by a delicate mucous epithelium, which is protected by different mechanisms, one being the continuous self-renewal from stem and precursor cells [2].The various parts of the intestine have different physiological functions concerning the digestion of food, the absorption of nutrients, and the excretion of fecal pellets.The number of bacteria drastically increases towards the colon [3,4].For example, the distal ileum contains about 10 8 bacteria per milliliter of luminal content and the colon contains about 10 11 [5].The mucosa-associated microbiota differ along the intestinal tract, including at least three different bacterial ecosystems with significant differences between the distal ileum and caecum, and also between the ascending colon and the transverse colon [6].The large number of bacteria in the colon is likely the reason why it is protected by a two-layered mucus barrier, the inner layer not being penetrable for bacteria [5,7].In contrast, the small intestine is covered by a single mucus layer [5].The predominant mucin in the murine intestine is Muc2, which is a typical secretory product of different types of goblet cells [8].Mucin glycosylation is an important element in the regulation of the intestinal microbiota.In mice, the small intestine is dominated by sialylated glycans, whereas in the colon, fucosylation dominates [9].Other typical secretory products of intestinal goblet cells are the trefoil factor family (TFF) peptide, Tff3; IgG Fc binding protein (Fcgbp); and the calcium-activated chloride channel regulator 1 and metalloprotease Clca1 (previously: Gob5) [10][11][12][13].Remarkably, at least in humans, TFF3 and FCGBP form disulfide-linked heteromers [10] and there are multiple indications that FCGBP and TFF3-FCGBP play a key role in the innate immune defense of mucous epithelia [12,14,15].Furthermore, the protein disulfide isomerase, Agr2, is essential for the production of mucus [16].It is located in the endoplasmic reticulum and also occurs in a secreted form [17].
Another source of intestinal mucous protection are the Brunner glands, which are localized in the proximal duodenum only, and are usually not found beyond the entrance of the pancreatic duct [18].As a hallmark, they secrete the mucin Muc6, which contains the unusual terminal carbohydrate moiety GlcNAcα1→4Galβ1→R (review: [19]).The key enzyme for the synthesis of the αGlcNAc residue is α1,4-N-acetylglucosaminyltransferase encoded by the A4gnt gene [19].The αGlcNAc residue is recognized by the lectin GSA-II from Griffonia simplicifolia [20].Of particular note, the TFF peptide Tff2 is a lectin, which binds to GlcNAcα1→4Galβ1→R and physically stabilizes the mucus barrier by crosslinking Muc6 (review: [21]).The combined expression of the Muc6/A4gnt/Tff2 system is not restricted to Brunner glands, but is also observed in gastric mucous neck and antral gland cells (MNCs, AGCs), and is conserved from frog to human [19][20][21][22].This explains why Tff2 and Muc6 are co-localized in the gastric mucus [23].
TFF peptides are evolutionary old lectins with important roles in mucosal protection and repair (reviews: [33][34][35]).They even occur in the skin and gastrointestinal (GI) tract of the frog Xenopus laevis [36,37].The most prominent phenotype in mice has been observed after inactivation of the Tff1 gene (Tff1 KO mice) [38].These animals obligatorily develop antral/pyloric adenoma and carcinomas have been detected in about 30% [38].Thus, Tff1 is considered as an antral tumor suppressor (reviews: [39,40]).Expression profiling of the stomach revealed significant differences in Tff1 KO mice when compared with the corresponding wild-type animals [41].Remarkably, also intestinal abnormalities have been reported for Tff1 KO mice, i.e., enlarged villi and abnormal infiltration of lymphoid cells [38,39].Thus, we expanded our previous studies [41] to the intestine.Notably, Tff1 expression has not been detected in the adult murine intestine in the past [42].However, delivery of Tff1 via engineered Lactococcus lactis or via the transgenic expression of TFF1 was found to increase resistance to intestinal damage in mice [43,44].Here, we present the systematic expression profiling of six different regions of the murine intestine and compare Tff1 KO mice with the corresponding wild-type littermates at the age of six weeks.The focus is on genes involved in various mucosal protection systems.Furthermore, we present protein data concerning Tff1 in the duodenum and Tff2 in the caecum/colon.genes involved in various mucosal protection systems.Furthermore, we present protein data concerning Tff1 in the duodenum and Tff2 in the caecum/colon.
The most significant differences between wild-type and Tff1 KO mice were observed for the following genes (other than Tff1): Gkn1, Gkn2, Clca1 (previously: Gob5), Lgr5, Nox1, Spdef, and Tff2.Here, differences were considered as being relevant when significance (*) was observed in at least two different regions or high significance (**) was observed in at least one region.

Expression Profiling of Tffs in the Murine Pancreas, Liver, and Lung (RT-PCR Analysis)
In the past, the expression of Tff genes was repeatedly documented to be changed in Tff1 KO mice as shown for the stomach [38,41] and the intestine (Figure 1).In order to complete these studies concerning other organs known for their Tff expression, we also investigated the pancreas, liver, and lung (Figure 2).
The most significant differences between wild-type and Tff1 KO mice were observed for the following genes (other than Tff1): Gkn1, Gkn2, Clca1 (previously: Gob5), Lgr5, Nox1, Spdef, and Tff2.Here, differences were considered as being relevant when significance (*) was observed in at least two different regions or high significance (**) was observed in at least one region.

Expression Profiling of Tffs in the Murine Pancreas, Liver, and Lung (RT-PCR Analysis)
In the past, the expression of Tff genes was repeatedly documented to be changed in Tff1 KO mice as shown for the stomach [38,41] and the intestine (Figure 1).In order to complete these studies concerning other organs known for their Tff expression, we also investigated the pancreas, liver, and lung (Figure 2).In the pancreas, both Tff2 and Tff3 expression were significantly reduced in Tff1 KO mice.In contrast, in the liver of Tff1 KO mice, only Tff3 expression was significantly decreased, whereas in the lung of Tff1 KO mice, only Tff2 expression was significantly down-regulated.

Protein Analysis of the Murine Duodenum
As Tff1 transcripts were not detected in the small or large intestine in the past [42], we checked the positive RT-PCR results concerning Tff1 (Figure 1) on the protein level.Here, a duodenal extract was separated via SEC, as reported previously [22], and analyzed for its Tff1 content (Figure 3).As a positive control, the Tff3 content was also determined.
In the pancreas, both Tff2 and Tff3 expression were significantly reduced in Tff1 KO mice.In contrast, in the liver of Tff1 KO mice, only Tff3 expression was significantly decreased, whereas in the lung of Tff1 KO mice, only Tff2 expression was significantly downregulated.

Protein Analysis of the Murine Duodenum
As Tff1 transcripts were not detected in the small or large intestine in the past [42], we checked the positive RT-PCR results concerning Tff1 (Figure 1) on the protein level.Here, a duodenal extract was separated via SEC, as reported previously [22], and analyzed for its Tff1 content (Figure 3).As a positive control, the Tff3 content was also determined.
Figure 3. Analysis of a murine duodenal extract (complete duodena from four animals).The elution profile after SEC on a Superdex 75 HL column as well as the distribution of Tff2 have been reported previously [22].(A) Distribution of the relative Tff1 (black) and Tff3 contents (green) as determined via Western blot analysis under reducing conditions and semi-quantitative analysis of monomeric band intensities.For Tff1, a regular band (black drawn line) and a somewhat shortened band (black dashed line) were analyzed separately.For comparison, the fractions were analyzed for their mucin content using the PAS reaction (pink); (B) 15% SDS-PAGE under reducing (R) and non-reducing (NR) conditions (post-in-gel reduction), respectively, and Western blot analysis of the high-molecular-mass fraction B8 and the low-molecular-mass fractions D3 and D5 concerning Tff1.As a control, fraction D1 from a murine stomach extract (St; [22]) was analyzed.(C) Analysis of the highmolecular-mass fractions B8-B10 concerning Tff3; (D) 1% AgGE and Western blot analysis of the high-molecular-mass fractions B8 and B9 concerning Tff3, Fcgbp, and Tff1, respectively (D, duodenal extract; Cae+C, extract from caecum plus total colon).Relative standard: DNA ladder (base pairs).Two Tff1 forms were detectable.In the high-molecular-mass region, it was mainly a slightly shortened Tff1 band that was found under reducing conditions (Figure 3A,B), which is barely detectable under non-reducing conditions, indicative of a disulfide-linked heterodimer (Figure 3B).In contrast, in the low-molecular-mass region, a regular and a shortened band were present (Figure 3A,B), which were both detectable also under nonreducing conditions, indicative of monomeric Tff1 (Figure 3B).
Tff3 appeared mainly in a high-molecular-mass form, and only minute amounts of a low-molecular-mass form were present (Figure 3A).From the high-molecular-mass form, Tff3 could be released after reduction (Figure 3C), and this band was partially shifted after non-reducing SDS-PAGE (arrow in Figure 3C).Two Tff1 forms were detectable.In the high-molecular-mass region, it was mainly a slightly shortened Tff1 band that was found under reducing conditions (Figure 3A,B), which is barely detectable under non-reducing conditions, indicative of a disulfide-linked heterodimer (Figure 3B).In contrast, in the low-molecular-mass region, a regular and a shortened band were present (Figure 3A,B), which were both detectable also under non-reducing conditions, indicative of monomeric Tff1 (Figure 3B).
Tff3 appeared mainly in a high-molecular-mass form, and only minute amounts of a low-molecular-mass form were present (Figure 3A).From the high-molecular-mass form, Tff3 could be released after reduction (Figure 3C), and this band was partially shifted after non-reducing SDS-PAGE (arrow in Figure 3C).
In order to verify the different Tff1 immunoreactive bands in the high-and lowmolecular-mass regions (Figure 3A,B), the corresponding bands from fractions B8, D1, D3, and D5 (Figure 4A,B) were eluted and Tff1 was identified via bottom-up proteomics (Figure 4C).As a reference for the Tff1 sequence, Tff1 was isolated from the high-molecularmass region of a murine stomach extract described previously [22] and analyzed in parallel (Figure 4C).For comparison, Tff3 was also identified in band B8 (Figure 4C).
In order to verify the different Tff1 immunoreactive bands in the high-and low-molecular-mass regions (Figure 3A,B), the corresponding bands from fractions B8, D1, D3, and D5 (Figure 4A,B) were eluted and Tff1 was identified via bottom-up proteomics (Figure 4C).As a reference for the Tff1 sequence, Tff1 was isolated from the high-molecularmass region of a murine stomach extract described previously [22] and analyzed in parallel (Figure 4C).For comparison, Tff3 was also identified in band B8 (Figure 4C).

Protein Analysis of the Murine Large Intestine
In contrast to the positive RT-PCR analysis presented in Figure 1, Tff2 mRNA was not observed in the murine large intestine in the past [42].Thus, we checked Tff2 synthesis in the large intestine on protein level (Figure 5).As a positive control, the Tff3 content was analyzed.
Tff2 was exclusively detectable in a high-molecular-mass form (Figure 5A), which can be released under reducing as well as non-reducing conditions (Figure 5B).This highmolecular-mass region also contains Muc6, as detected via staining with the lectin GSA-II (Figure 5C).Furthermore, the presence of Tff2 was verified via bottom-up proteomics in the high-molecular-mass fraction B8 after reducing SDS-PAGE (Figure 5D,E).

Protein Analysis of the Murine Large Intestine
In contrast to the positive RT-PCR analysis presented in Figure 1, Tff2 mRNA was not observed in the murine large intestine in the past [42].Thus, we checked Tff2 synthesis in the large intestine on protein level (Figure 5).As a positive control, the Tff3 content was analyzed.
Tff2 was exclusively detectable in a high-molecular-mass form (Figure 5A), which can be released under reducing as well as non-reducing conditions (Figure 5B).This highmolecular-mass region also contains Muc6, as detected via staining with the lectin GSA-II (Figure 5C).Furthermore, the presence of Tff2 was verified via bottom-up proteomics in the high-molecular-mass fraction B8 after reducing SDS-PAGE (Figure 5D,E).
Tff3 exists in a high-molecular-mass form (Figure 5A).After AgGE, this form was mainly identified as Tff3-Fcgbp (Figure 3D).However, there was also a second Tff3-positive band with a somewhat lower molecular mass, which was not positive with the anti-Fcgbp antiserum used (Figure 3D).
Tff3 exists in a high-molecular-mass form (Figure 5A).After AgGE, this form was mainly identified as Tff3-Fcgbp (Figure 3D).However, there was also a second Tff3-positive band with a somewhat lower molecular mass, which was not positive with the anti-Fcgbp antiserum used (Figure 3D).

Expression Profiling along the Murine Intestinal Tract: Indications for Different Mucosal Protection Systems
As controls, expression profiles of the genes encoding the transcription factors Cdx1, Cdx2, and Pdx1 were monitored (Figure 1).Expression of the intestine-specific genes Cdx1 and Cdx2 is rather uniform with a slight upregulation in the colon.In contrast, the transcription factor Pdx1 is known to regulate, in particular, endocrine differentiation in the gastric antrum, pancreas, and duodenum [46], which is in agreement with its expression in all three regions of the duodenum.Furthermore, expression of the hormone gastrin, particularly in the proximal duodenum, is as expected.
Generally, the expression profiling of genes encoding proteins known to play a role in the mucosal innate immune defense, such as Tff peptides, gastrokines, mucins, Fcgbp,

Expression Profiling along the Murine Intestinal Tract: Indications for Different Mucosal Protection Systems
As controls, expression profiles of the genes encoding the transcription factors Cdx1, Cdx2, and Pdx1 were monitored (Figure 1).Expression of the intestine-specific genes Cdx1 and Cdx2 is rather uniform with a slight upregulation in the colon.In contrast, the transcription factor Pdx1 is known to regulate, in particular, endocrine differentiation in the gastric antrum, pancreas, and duodenum [46], which is in agreement with its expression in all three regions of the duodenum.Furthermore, expression of the hormone gastrin, particularly in the proximal duodenum, is as expected.
Generally, the expression profiling of genes encoding proteins known to play a role in the mucosal innate immune defense, such as Tff peptides, gastrokines, mucins, Fcgbp, and enzymes involved in the metabolism of ROS, point to different mucosal protection systems along the murine intestinal tract.On the one hand, secretory products of goblet cells protect the entire intestine (basic protection system).On the other hand, there are additional, specific protection systems, particularly in the proximal duodenum as well as the proximal colon.Genes encoding typical secretory proteins of goblet cells, i.e., Clca1 (previously: Gob5), Fcgbp, Muc2, Tff3, and Zg16, show a rather uniform expression profile along the murine intestine (Figure 1).This result is not surprising as it reflects the common view that the complete intestine is protected by a mucus barrier produced by goblet cells, Muc2 being the predominant mucin.Another known component is the Tff3-Fcgbp heteromer, which has been demonstrated, e.g., in duodenal and colonic extracts (Figure 3D), and presumably plays a role for the mucosal innate immune defense [14,47].
For comparison, Tff3 was identified via proteomics in the high-molecular-mass Tff3-Fcgbp complex of the duodenum after reduction (Figure 4C).Because of its abundance, the complete Tff3 sequence could be determined, indicating for the first time, unambigously cleavage of the signal peptidase after Ala-23 of the precursor.
Notably, the expression of Spdef increases towards the colon with an additional peak in the proximal duodenum (Figure 1).This might be due to the increasing percentage of goblet cells relative to the total number of epithelial cells from the duodenum to the colon, as the transcription factor Spdef regulates terminal differentiation of goblet cells [48].The peak in the proximal duodenum is caused by additional Spdef expression in Brunner glands [48].

Specific Protection of the Proximal Duodenum and the Colon by the Tff2/Muc6 Complex
In the intestine, Tff2, Muc6, and A4gnt are predominantly expressed in the proximal duodenum (Figure 1).This is in agreement with their known synthesis in Brunner glands [19,22,42], which are located in the proximal duodenum only and are usually not found beyond the entrance of the pancreatic duct [18,49].Thus, co-expression of Tff2, Muc6, and A4gnt in Brunner glands allows formation of a lectin-mediated, high-molecular mass Tff2/Muc6 complex, as already demonstrated on the protein level in murine duodenal extracts [22].When compared with the murine stomach [41], the expression of Tff2, Muc6, and A4gnt in the intestine is much lower.
Of particular note, the expression of Tff2, Muc6, and A4gnt was also detectable in the proximal colon (Figure 1).In the past, there were contradictory reports concerning Tff2 transcripts in the murine colon.They were either not detected [42] or described as being expressed in colonic epithelial cells [50], which might reflect differences between different mouse strains.However, immunohistochemistry of the proximal colon of rats localized Tff2 strongly in the lower parts of the crypts [51].Thus, we tested an extract from caecum plus total colon for the presence of Tff2 (Figure 5).We could clearly identify a highmolecular-mass Tff2/Muc6 complex (Figure 5A), which was positive for GSA-II (Figure 5C), indicative of terminal GlcNAc residues in Muc6 due to A4gnt activity (Figure 5C).Tff2 was also identified via proteomics (Figure 5E).Notably, we could identify Tff2 not only in band 1, which is equivalent to the band strongly immunoreactive for Tff2 (Figure 5D), but Tff2 was also clearly identified in a band below with only weak immunoreactivity (designated as band 2: Figure 5D).This band can also be seen in Figure 5B and probably represents a shortened variant, maybe missing a few amino acid residues at the N-or C-terminal.However, the question of the cellular origin of this Tff2/Muc6 complex arises, as goblet cells are not known for the synthesis of Tff2.
In the past, the unusual GlcNAc-residue typical of Muc6 was recognized in gastric MNCs and AGCs as well as in Brunner glands, but also in the deep crypt cells of the rat colon [52,53].These cells were first described by Altmann as "deep crypt secretory (DCS)" cells, particularly in the rat ascending and transverse colon, which originate from precursor cells and typically secrete mucus [54].Later on, these cells were recognized again, due to their expression of Agr2 (previously termed Gob4) and typical staining with Alcian blue [16,55].Notably, these Alcian blue positive DCS cells can be clearly distinguished from goblet cells, the latter being characterized by the synthesis of Tff3 [16].Alcian blue is known to stain acidic mucins, such as Muc6 in gastric MNCs and AGCs, which are the characteristic Agr2-expressing cells in the murine stomach [56].This implies that the disulfide isomerase Agr2, probably together with the disulfide isomerases Pdia3 and Pdia6 [57], plays a major role in the folding of Muc6, particularly in gastric MNCs and AGCs, as well as in duodenal Brunner glands and colonic DCS cells.This assumption is supported by the RT-PCR analysis concerning Agr2, which peaks in the proximal duodenum and proximal colon (Figure 1).It is also in line with a recent report describing Agr2 as a marker of colonic DCS cells, whose expansion is regulated by interleukin (IL)-13 originating from type 2 innate lymphoid cells (ILC2) [58].However, Agr2 has been reported to occur in all intestinal secretory cell types [16], but reaches its highest level in the proximal colon [17].Taken together, the synthesis of a Tff2/Muc6 complex in the DCS cells of the colon is comparable with the situation in the gastric antrum (review: [40]).Of particular note, Lgr5 + stem cells are located at the base of both the colonic crypts and antral glands [59].Thus, it is tempting to speculate that the Tff2/Muc6 complex protects these basal stem cells in the colon from microbial colonization via a highly viscous mucous plug.This is in agreement with the view that DCS cells are important components of the colonic stem cell niche [60].In contrast, the Lgr5 + stem cells in the small intestine are protected by secretory products of the neighboring Paneth cells, which are lacking in the colon [59].

Specific Protection of the Duodenum by Tff1 and Gastrokines
Tff1 and the gastrokine genes Gkn1, Gkn2, and Gkn3 are also expressed selectively in the duodenum (Figure 1), but at much lower levels when compared with the stomach [41].The intestinal expression of Tff1 was somewhat surprising, as Tff1 transcripts were neither detected in the small nor the large intestine in the past [42].As the expression of Tff1 and gastrokines is not confined to the proximal duodenum, expression in goblet cells might be possible.This view is supported by the observation that Tff1 exists in a high-molecularmass form (Figure 3A), which has been identified as Tff1-Fcgbp heterodimer (Figure 3D), Fcgbp being typically secreted by goblet cells.Tff1-Fcgbp has already been described as occurring in the murine as well as the human stomach [41,61].Thus far, it is not clear why Tff1-Fcgbp is mainly present in the duodenum and hardly detectable in the colon (Figure 3D); a possible reason may be different types of goblet cells, which differ in their Tff1 synthesis.
Proteomics clearly verified the existence of different Tff1 entities in the duodenum in both the high-and low-molecular-mass range (Figure 4).The high-molecular-mass form mainly exists in a shortened variant (Figure 3A,B and Figure 4A).The low-molecularmass forms consist of a normal and shortened forms, the latter missing up to seven amino acid residues at least at the N-terminal (bands D3b and D5; Figure 4C,D) when compared with the longest Tff1 form from the murine stomach as a reference (Figure 4C,D).The heterogeneities at the N-terminal of Tff1 in the duodenum as well the stomach are remarkable (Figure 4D).In the stomach, the Tff1 precursor is preferentially cleaved by signal peptidase after Ala-21 or after Ala-23, liberating an unusual N-terminal repetitive sequence starting with a pyro-Glu residue (qAQAQAQAQE. . .and qAQAQAQE. .., respectively, Figure 4D), due to cyclization of an N-terminal Gln residue with the help of glutaminyl cyclase.Currently, it is not clear how the multiple N-terminally-truncated Tff1 forms in the duodenum are generated (Figure 4D); it is possible that alternative cleavages by signal peptidase occur after various Gln or Ala residues and degradation by aminopeptidases.Notably, two forms were identified also starting with a pyro-Glu residue (qAQAQE. .., qAQE. .., Figure 4D), indicating cleavage by signal peptidase after Ala-25 and Ala-27, respectively.However, artificial cyclization in the electrospray ionization source cannot be excluded [62].
However, only Gkn3 expression is significantly higher in the proximal duodenum when compared with the medial and distal parts, which is in line with its documented expression in Brunner glands [63].As Gkn3 is characteristically co-expressed with Tff2 and Muc6, not only in Brunner glands but also in gastric MNCs and AGCs [30,63], it might be possible that Gkn3 supports the protective Tff2/Muc6 complex via a yet unknown mechanism.

Specific Protection of the Proximal Duodenum and the Colon by Epithelial Fucosylation
As a hallmark, Fut2 is predominantly expressed in the proximal duodenum and the proximal colon (Figure 1).Fut2 regulates fucosylation of intestinal epithelial cells.On the one hand, epithelial L-fucose is used as a dietary carbohydrate for many bacteria.On the other hand, fucosylation inhibits infection, e.g., from Salmonella typhimurium [64].Of particular note, microbiota induce intestinal epithelial fucosylation by triggering Fut2 expression [65].For example, Bacteroides have been shown to induce epithelial fucosylation by direct interaction [66][67][68][69].In addition, Fut2 expression can also be mediated indirectly by interleukin (IL)-22 and lymphotoxin α originating from type 3 innate lymphoid cells (ILC3) [64].Fucosylation can, e.g., change the signaling of receptors such as TLR4 in the murine colon, which is essential for recovery from mucosal injury in vivo [65].Furthermore, goblet cells can be distinguished according to their fucosylation pattern, such as intercrypt goblet cells in the colon [70].In contrast, fucosylation deficiency in mice leads to colitis and adenocarcinoma [71].
Bacteroides have been shown to accumulate in the murine proximal duodenum and also the colon [6].Starting with the caecum, anaerobic genera appear in the murine lower alimentary tract and there is an increase in the richness (amount of different phylotypes) at this point [6].These could be the reasons why Fut2 expression peaks in the proximal duodenum and the proximal colon (Figure 1).

Specific Protection of the Proximal Duodenum and Particularly the Colon by ROS-Generating Enzymes
Extracellular ROS such as the relatively stable (10 −2 -10 −3 s) but weak oxidant H 2 O 2 and the instable (10 −5 s) superoxide are not only used by immune cells but also by mucous epithelia for innate immune defense against microorganisms [24][25][26].H 2 O 2 is diffusible and preferentially reacts with thiols from cysteine residues, whereas the superoxide anion cannot diffuse through membranes [25].In the gastrointestinal tract, Duox2 typically generates extracellular H 2 O 2 [25,26,72], which is used by Lpo to produce the highly microbicidal hypothiocyanite (DUOX/H 2 O 2 /LPO/SCN − system) [28].Of particular note, Duox2 and Lpo are differentially expressed in the colonic crypts of mice, i.e., Duox2 expression is located at the upper crypt quintile, whereas Lpo transcripts are present in the basal quintile, where stem cells reside [73].
Expression of Lpo in the proximal colon (Figure 1) is an indication for a need for specific protection of these locations, controlling non-invasive pathogen colonization of the mucus, and might complement other protection systems such as fucosylation.The appearance of anaerobic genera and the increasing richness, particularly in the colon [6], would easily explain the large amounts of both Duox2 and Lpo needed in order to generate sufficient microbicidal SCNO − .Furthermore, maximal expression of Nox1 in the proximal colon (Figure 1) is in line with the drastically increasing number of bacteria in this region and fits with previous reports [25,26].Notably, Nox1 is most highly expressed in the lower two thirds of colon crypts [74] and thus might be specifically suited to protect the stem and precursor cells and/or enhance regeneration processes.However, the superoxide generated by Nox1 can be used to produce SCNO − only in the presence of Sod3 and Lpo; the latter is also expressed at the basal quintile of the crypts [73].
Sod3 is typically present in luminal fluids as well as in the extracellular matrix and protects against oxidative stress-induced injury.Thus, it is not surprising that the entire intestine is protected by Sod3 (Figure 1).For comparison, the expression of Sod1 and Sod2 were also determined (Figure 1), encoding intracellular superoxide dismutases.These transcripts are far more abundant than that of Sod3 and were detectable all along the intestine.

Summary of the Different Mucosal Protection Systems along the Murine Intestine
In Figure 6, the different protection systems of the murine intestine are summarized.Clearly, besides a basic protection by goblet cell products along the entire intestinal tract, specific systems have evolved, particularly protecting the proximal duodenum (mainly Brunner glands secretions) and the proximal colon (particularly secretory products of DCS cells).The distal colon was not studied here.
and/or enhance regeneration processes.However, the superoxide generated by Nox1 can be used to produce SCNO − only in the presence of Sod3 and Lpo; the latter is also expressed at the basal quintile of the crypts [73].
Sod3 is typically present in luminal fluids as well as in the extracellular matrix and protects against oxidative stress-induced injury.Thus, it is not surprising that the entire intestine is protected by Sod3 (Figure 1).For comparison, the expression of Sod1 and Sod2 were also determined (Figure 1), encoding intracellular superoxide dismutases.These transcripts are far more abundant than that of Sod3 and were detectable all along the intestine.

Summary of the Different Mucosal Protection Systems along the Murine Intestine
In Figure 6, the different protection systems of the murine intestine are summarized.Clearly, besides a basic protection by goblet cell products along the entire intestinal tract, specific systems have evolved, particularly protecting the proximal duodenum (mainly Brunner glands secretions) and the proximal colon (particularly secretory products of DCS cells).The distal colon was not studied here.

Transcriptional Changes in the Intestine of Tff1 KO Mice
Major changes in Tff1 KO mice were the significantly increased expression of both Gkn1 and Gkn2 in the proximal duodenum (Figure 1).Based on the reported mitogenic activity of a Gkn1 fragment [75], an expected higher Gkn1 concentration might be responsible for the thickened villi of the small intestinal mucosa and the presence of inflammatory cells, as described previously [38].However, macroscopically, we could observe intestinal abnormalities in the proximal duodenum only, i.e., a thickening.The significantly increased expression of Gkn2 in Tff1 KO mice might result in secretion of a disulfide-linked Gkn2 homodimer as being detectable in the gastric antrum of Tff1 KO mice [41].The biological function of such a Gkn2 form, particularly in Tff1 KO mice, is not known currently.
An additional hallmark is the significant down-regulation of Tff2 in the proximal colon of Tff1 KO mice (Figure 1).Theoretically, this could diminish protection of the colonic stem cells by the Tff2/Muc6 complex and could lead to increased susceptibility of Tff1 KO mice to DSS-induced colitis, similarly to what has been reported for Tff2 KO mice [76].However, Tff1 KO

Transcriptional Changes in the Intestine of Tff1 KO Mice
Major changes in Tff1 KO mice were the significantly increased expression of both Gkn1 and Gkn2 in the proximal duodenum (Figure 1).Based on the reported mitogenic activity of a Gkn1 fragment [75], an expected higher Gkn1 concentration might be responsible for the thickened villi of the small intestinal mucosa and the presence of inflammatory cells, as described previously [38].However, macroscopically, we could observe intestinal abnormalities in the proximal duodenum only, i.e., a thickening.The significantly increased expression of Gkn2 in Tff1 KO mice might result in secretion of a disulfide-linked Gkn2 homodimer as being detectable in the gastric antrum of Tff1 KO mice [41].The biological function of such a Gkn2 form, particularly in Tff1 KO mice, is not known currently.
An additional hallmark is the significant down-regulation of Tff2 in the proximal colon of Tff1 KO mice (Figure 1).Theoretically, this could diminish protection of the colonic stem cells by the Tff2/Muc6 complex and could lead to increased susceptibility of Tff1 KO mice to DSS-induced colitis, similarly to what has been reported for Tff2 KO mice [76].However, Tff1 KO mice show the same response in a DSS colitis model as wild-type mice [50].Thus, one might speculate that the significant up-regulation of Nox1 expression, particularly in the proximal colon of Tff1 KO mice (Figure 1), could compensate for the reduced protection from bacterial colonization by the Tff2/Muc6 complex.
There is a tendency for up-regulated Tff3 expression in the duodenum and the proximal colon of Tff1 KO mice (Figure 1), which is in agreement with a previous report [38].Furthermore, also gastrin expression shows a tendency for up-regulation in the duode-num of Tff1 KO mice (Figure 1).This is in contrast to the antrum, where Tff1 KO mice show statistically significant down-regulation of Gast [41].
Notably, there is a generally up-regulated expression of Clca1 (previously Gob5) and Lgr5 in the entire intestine of Tff1 KO mice, which is significant for both genes in the ileum and colon, and additionally for Lgr5 in the proximal duodenum (Figure 1).The upregulation of the stem cell marker, Lgr5, would be in agreement with reports that TFF1 is able to control cell differentiation by regulating the balance between cell proliferation and death (anti-proliferative and anti-apoptotic effects of TFF1) [38,[77][78][79].Furthermore, Agr2 and Sod1 also show a tendency toward up-regulation in Tff1 KO mice (Figure 1).Upregulation of Agr2 might be a response to latent endoplasmic reticulum (ER) stress [80,81], which could be due to the unfolded protein response (UPR) activated in Tff1 KO mice [82].

Tff Expression Is
Cross-Regulated in the Pancreas, Liver, and Lung of Tff1 KO

Mice
In the past, coordinate regulation of Tff genes was observed (review [83]), which is due to the clustered organization of the three Tff genes in a head-to-tail orientation within a 40 kb region on chromosome 17q [84].For example, Tff2 expression is significantly downregulated in the stomach of Tff1 KO mice, particularly in the corpus [38,41,85].Generally, the linear organization of the Tff genes reflects their spatial distribution along the GI tract, strongly suggesting the existence of a locus control region [84].This region may be affected in the Tff1 KO mice, resulting in down-regulation of Tff2 and Tff3, particularly in organs of the GI tract.However, epigenetic mechanisms play a major role in the regulation of Tff expression [84].
Here, we show that Tff2 is also significantly down-regulated in the pancreas and the lung of Tff1 KO animals (Figure 2).In both of these organs, Tff2 is the predominant TFF peptide.The down-regulation of pancreatic Tff2 expression is in agreement with a previous report [38].Loss of pancreatic Tff2 has been shown to promote formation of intraductal papillary mucinous neoplasms in mice [86].Thus, it might be possible that Tff1 KO mice exhibit a similar phenotype due to a secondary effect.Furthermore, as Tff2 KO mice were reported to have compromised lung structure and function [87], it would also be interesting to investigate the lung of Tff1 KO animals in detail.
In the liver of Tff1 KO mice, Tff3 expression is significantly down-regulated (Figure 2).Tff3 is moderately expressed in biliary epithelial cells [88] and dramatic down-regulation of Tff3 expression was observed in a murine model of type II diabetes [89].Tff3 downregulation is also correlated with a fatty-liver phenotype [90].Furthermore, Tff3 KO mice show altered liver lipid metabolism [91].Thus, it would be interesting to check if a similar phenotype occurs in Tff1 KO mice.

Animals
Animal care and experimental procedures were conducted in compliance with the Directive 2010/63/EU of the European parliament and of the council of 22 September 2010 on the protection of animals used for scientific purposes, the German Animal Welfare Act, and the regulations on the welfare of animals used for experiments or for other scientific purposes in their currently valid versions.In the course of these studies, Tff1 KO mice and their corresponding wild-type littermates (mixed 129/Sv and C57BL/6 background) described previously [41,92] were investigated at the age of six weeks (Landesverwaltungsamt Sachsen-Anhalt; license number: 203.m-42502-2-1722UniMD; 18 May 2022).Mice heterozygous for Tff1 were originally obtained from Dr. M.-C.Rio and Dr. C. Tomasetto (IGBMC, Illkirch, France) [38].Furthermore, for protein analysis, adult wild-type animals with a mixed 129/Sv and C57BL/6 background at the age of 16 weeks were used as described previously described [6,93] (Animal Welfare Officer of the Medical Faculty of the Otto-von-Guericke University Magdeburg; license number: IMMC-TWZ-01; 1 January 2015).Semi-quantitative evaluation of the relative expression levels of the selected genes was performed using the GeneTools analysis software (Version 4.3.17.0,Syngene Bioimaging, Cambridge, UK), as previously described in detail [93].Generally, the relative intensities were normalized against the relative intensities of the Actb transcripts (intestine 23 or 24, liver 24, lung 21, and pancreas 27 amplification cycles, respectively) and the highest value (mean) within each series was set to 1.If no robust signal was obtained (Figure 2: Tff1, Tff2/liver), an external signal was used as standard and set to 1.The statistical analysis using Student's t-test was performed with the Excel 2019 software package (Microsoft, Syracuse, NY, USA).Error bars represent ±SEM.Significant differences between the mean values between wild-type and Tff1 KO mice are indicated by asterisks (p ≤ 0.05: significant, *; p ≤ 0.01: highly significant, **; p ≤ 0.001: extremely highly significant, ***).

SDS-PAGE, AgGE, and Western Blot Analysis
Denaturing SDS-PAGE under reducing and non-reducing conditions, respectively, native AgGE, and Western blot analysis were described previously [10,41,98,99].When indicated, gels after non-reducing SDS-PAGE were subjected to post-in-gel reduction with 1% mercaptoethanol at 50 • C for 2 min, according to a previous report [97].As a relative standard for non-denaturing AgGE, a DNA ladder was used as specified previously [15].

Identification of Proteins via Bottom-Up Proteomics
For protein identification, gel bands were excised and subjected to tryptic digestion, followed by liquid chromatography coupled to electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS).The data obtained were processed and analyzed with a search engine, as described in detail previously [15].For N-terminal glutamine residues, cyclization to pyroglutamic acid (pyro-Glu) was also taken into account.This is a posttranslational modification, which is typical of some TFF peptides, but cyclization of free Gln and Glu can also occur in the electrospray ionization source [62].

Conclusions
In this study, different mucosal protection systems were systematically localized along the murine intestine (Figure 6).Remarkably, the evolutionary old Muc6/A4gnt/Tff2 system is not restricted to the stomach and Brunner glands, but also protects the deep crypts, particularly of the proximal colon.In the latter, the expression of Nox1 and of Lpo also culminate.A systematic investigation of the distinct parts of the colon is a future challenge.This might help to increase the understanding of the differences in the carcinogenesis in the distinct colonic regions.Furthermore, we characterized Tff1-Fcgbp heterodimers,

Figure 3 .
Figure3.Analysis of a murine duodenal extract (complete duodena from four animals).The elution profile after SEC on a Superdex 75 HL column as well as the distribution of Tff2 have been reported previously[22].(A) Distribution of the relative Tff1 (black) and Tff3 contents (green) as determined via Western blot analysis under reducing conditions and semi-quantitative analysis of monomeric band intensities.For Tff1, a regular band (black drawn line) and a somewhat shortened band (black dashed line) were analyzed separately.For comparison, the fractions were analyzed for their mucin content using the PAS reaction (pink); (B) 15% SDS-PAGE under reducing (R) and non-reducing (NR) conditions (post-in-gel reduction), respectively, and Western blot analysis of the high-molecular-mass fraction B8 and the low-molecular-mass fractions D3 and D5 concerning Tff1.As a control, fraction D1 from a murine stomach extract (St;[22]) was analyzed.(C) Analysis of the high-molecular-mass fractions B8-B10 concerning Tff3; (D) 1% AgGE and Western blot analysis of the high-molecular-mass fractions B8 and B9 concerning Tff3, Fcgbp, and Tff1, respectively (D, duodenal extract; Cae+C, extract from caecum plus total colon).Relative standard: DNA ladder (base pairs).

Figure 4 .
Figure 4. Proteome analysis of the high-and low-molecular-mass forms of Tff1 in a duodenal extract (fractions B8, and D1, D3, and D5 from Figure 3).(A,B) SDS-PAGE under reducing conditions of the high-molecular mass fractions B7-B10 (A) and the low-molecular-mass fractions C12-D7 (B) and Western blot analysis concerning Tff1.Fractions B8, D1, D3, and D5 were then separated via preparative reducing of SDS-PAGE, and after Coomassie staining, bands termed B8, D1, D3a, D3b, and D5 were excised (marked in red).(C) Results of the proteome analyses after tryptic in-gel digestion of bands B8, D1, D3a, D3b, and D5.Identified regions in Tff1 are shown in red.In B8, Tff3 was also identified.The results of the Tff1 reference (from a stomach extract) are also shown.The longest Nterminal sequences identified are shown.(D) Identification of heterogeneous Tff1 N-terminal sequences in bands D3b and the stomach reference (q indicates a pyro-Glu residue).The predominant sequences are underlined.

Figure 4 .
Figure 4. Proteome analysis of the high-and low-molecular-mass forms of Tff1 in a duodenal extract (fractions B8, and D1, D3, and D5 from Figure 3).(A,B) SDS-PAGE under reducing conditions of the high-molecular mass fractions B7-B10 (A) and the low-molecular-mass fractions C12-D7 (B) and Western blot analysis concerning Tff1.Fractions B8, D1, D3, and D5 were then separated via preparative reducing of SDS-PAGE, and after Coomassie staining, bands termed B8, D1, D3a, D3b, and D5 were excised (marked in red).(C) Results of the proteome analyses after tryptic in-gel digestion of bands B8, D1, D3a, D3b, and D5.Identified regions in Tff1 are shown in red.In B8, Tff3 was also identified.The results of the Tff1 reference (from a stomach extract) are also shown.The longest N-terminal sequences identified are shown.(D) Identification of heterogeneous Tff1 N-terminal sequences in bands D3b and the stomach reference (q indicates a pyro-Glu residue).The predominant sequences are underlined.

Figure 5 .
Figure 5. Analysis of a murine caecum plus total colon extract (single individual).(A) Elution profile after SEC on a Superdex 75 HL column as determined via absorbance at 280 nm (PAS-positive mucin fractions: pink).Underneath: distribution of the relative Tff2 (red) and Tff3 contents (green) as determined via Western blot analysis under reducing conditions and semi-quantitative analysis of the monomeric band intensities; (B) 15% SDS-PAGE under reducing (R) and non-reducing (NR) conditions (post-in-gel reduction), respectively, and Western blot analysis of the high-molecular-mass fractions B8-B10 concerning Tff2; (C) 1% AgGE and Western blot analysis of the fractions B6-C2 concerning Muc6 (lectin GSA-II).Relative standard: DNA ladder (base pairs).(D) SDS-PAGE under reducing conditions of fraction B8.Shown is a Western blot analysis concerning Tff2 and in parallel, Coomassie staining.Bands 1 and 2 were excised for proteome analysis.(E) Results of the proteome analysis after tryptic in-gel digestion of bands 1 and 2. Identified regions in Tff2 are shown in red.

Figure 5 .
Figure 5. Analysis of a murine caecum plus total colon extract (single individual).(A) Elution profile after SEC on a Superdex 75 HL column as determined via absorbance at 280 nm (PAS-positive mucin fractions: pink).Underneath: distribution of the relative Tff2 (red) and Tff3 contents (green) as determined via Western blot analysis under reducing conditions and semi-quantitative analysis of the monomeric band intensities; (B) 15% SDS-PAGE under reducing (R) and non-reducing (NR) conditions (post-in-gel reduction), respectively, and Western blot analysis of the high-molecular-mass fractions B8-B10 concerning Tff2; (C) 1% AgGE and Western blot analysis of the fractions B6-C2 concerning Muc6 (lectin GSA-II).Relative standard: DNA ladder (base pairs).(D) SDS-PAGE under reducing conditions of fraction B8.Shown is a Western blot analysis concerning Tff2 and in parallel, Coomassie staining.Bands 1 and 2 were excised for proteome analysis.(E) Results of the proteome analysis after tryptic in-gel digestion of bands 1 and 2. Identified regions in Tff2 are shown in red.
mice show the same response in a DSS colitis model as wild-type mice [50].

Table 1 .
Oligonucleotides used for RT-PCR analysis and calculated size of the products.