Protective Effects of Currants (Vitis vinifera) on Corticolimbic Serotoninergic Alterations and Anxiety-like Comorbidity in a Rat Model of Parkinson’s Disease

Parkinson’s disease (PD) is a progressive neurodegenerative disorder characterized by the loss of nigral dopaminergic neurons. Increasing evidence supports that PD is not simply a motor disorder but a systemic disease leading to motor and non-motor symptoms, including memory loss and neuropsychiatric conditions, with poor management of the non-motor deficits by the existing dopaminergic medication. Oxidative stress is considered a contributing factor for nigrostriatal degeneration, while antioxidant/anti-inflammatory properties of natural phyto-polyphenols have been suggested to have beneficial effects. The present study aimed to determine the contribution of monoaminergic neurotransmission on the anxiety-like phenotype in a rat rotenone PD model and evaluate the possible neuroprotective effects of black Corinthian currant, Vitis vinifera, consisting of antioxidant polyphenols. Rotenone-treated rats showed anxiety-like behavior and exploratory deficits, accompanied by changes in 5-HT, SERT and β2-ARs expression in the prefrontal cortices, hippocampus and basolateral amygdala. Importantly, the motor and non-motor behavior, as well as 5-HT, SERT and β2-ARs expression patterns of the PD-like phenotype were partially recovered by a supplementary diet with currants. Overall, our results suggest that the neuroprotective effects of Corinthian currants in rotenone-induced anxiety-like behavior may be mediated via corticolimbic serotonergic transmission.


Introduction
Parkinson's disease (PD) represents the second most common neurodegenerative disorder, affecting up to ten million people worldwide, with a predicted increase of more than two-fold by 2030 [1]. A major neuropathological hallmark of PD is the degeneration and subsequent loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc), resulting in a profound depletion of dopamine in striatal terminals that leads to clinical manifestations of resting tremor, bradykinesia, postural instability and rigidity. Increasing evidence suggests that PD is not simply a motor disorder but rather a disease with multiple non-motor symptoms, including neuropsychiatric and autonomic dysfunctions [2], occurring at early stages of PD [3]. Therapies currently available do not successfully address the non-motor manifestations, and studies on the underlying mechanisms would be of importance for early intervention strategies that would improve patients' outcomes.
In PD, the neurodegenerative process extends beyond the dopaminergic system with extensive extra-striatal pathology, involving neurotransmitters such as serotonin (5-HT) and norepinephrine [4]. Moreover, a high incidence of depression and anxiety characterizes PD psychopathology [5] suggesting a complex dysfunction of noradrenergic and serotonergic

Effects of Rotenone Treatment and Currant Consumption on Thigmotaxic Behavior Using the Open Field Test (OFT)
We used the OFT to evaluate the exploratory behavior of new environments and the innate fear in rats of open spaces that trigger anxiety. Their preference to remain longer in the periphery of the OFT in comparison to the central areas is considered a thigmotaxic response, and this was used as an index of anxiety in rats treated with vehicle and rotenone with or without a complementary diet with currants ( Figure 1A). Two-way repeated measures ANOVA indicated significant effects of treatment (F(1.44) = 239.848, p < 0.001) and interaction (treatment × currant, F(1.44) = 5.425, p = 0.025) but not a significant currant (F(1.44) = 0.04, p = 0.953) effect. Specifically, the duration of visits to the center zone of the OFT (during a period of 10 min) was significantly decreased in rotenonetreated rats (p < 0.001). In line with this, rotenone treatment with or without currant consumption resulted in a statistically significant reduction in the duration of the visits to the center zone of the OFT, when measured at 10, 24 and 38 (p < 0.001) days. Overall, thigmotaxis, the preference to the periphery and walls, was increased in rotenone-treated rats, as indicated by the reduced time spent in the center zone. Importantly, at day 38, a statistically significant decrease in thigmotaxis was observed in the rotenone group who consumed currants (p = 0.006) when compared with the rotenone group who consumed conventional food ( Figure 1A). reduced time spent in the center zone. Importantly, at day 38, a statistically significant decrease in thigmotaxis was observed in the rotenone group who consumed currants (p = 0.006) when compared with the rotenone group who consumed conventional food ( Figure  1A).

Effects of Rotenone Treatment and Currant Consumption on Behavioral Parameters in the Elevated Plus-Maze (EPM)
The EPM is a widely used behavioral assay for assessing the anxiety responses of rodents. To determine whether a supplementary diet with currant improved the anxietylike behavior of rotenone-treated rats, on day 38, rats were subjected to the elevated plus-maze test ( Figure 1B-D). Rotenone-treated animals showed significantly higher levels of anxiety-like behavior. Considering the time animals spent in open arms, two-way ANOVA indicated a significant effect of treatment (F(1.47) = 21.650, p < 0.001) but not of currant consumption (F(1.47) = 0.583, p = 0.449) nor interaction (treatment × currant, F(1.47) = 3.368, p = 0.073). Thus, the duration of visits to the open arms of the EPM (during a period of 5 min) was significantly decreased in rotenone-treated rats with (p = 0.027) or without (p = 0.001) currant intake ( Figure 1B). In line with this result, considering the duration of visits in closed arms, two-way ANOVA demonstrated significant effects of treatment (F(1.47) = 52.969, p < 0.001) and currant consumption (F(1.47) = 12.935, p = 0.001) but not significant interaction (treatment × currant, F(1.47) = 0.614, p = 0.437) ( Figure 1C). An independent t-Test revealed a significantly increased duration of visits in closed arms of both rotenone (p < 0.001), and rotenone-currant (p < 0.001) groups indicating their higher levels of anxiety. For the anxiety index, two-way ANOVA revealed a significant effect of treatment (F(1.47) = 24.334, p < 0.001) but not of currant consumption (F(1.47) = 0.008, p = 0.929) nor interaction (treatment × currant, F(1.47) = 0.264, p = 0.610). Overall, the anxiety index, calculated based on the number of visits and duration in open and closed arms was different in the four groups of rats with higher values in the rotenone-treated rats with (p = 0.016) or without (p < 0.001) currant intake ( Figure 1D). The latency to the first entry in the open arms was significantly prolonged in the rotenone group with (p = 0.046) or without (p < 0.001) a currant diet compared to the equivalent control group ( Figure 1E), with a statistically significant effect of treatment (F(1.47) = 25.382, p < 0.001) but not of currant consumption (F(1.47) = 1.897, p = 0.175) nor interaction (treatment × currant, F(1.47) = 3.299, p = 0.076).

Prefrontal Cortex, Hippocampus and Basolateral Amygdala 5-HT Immunoreactivity Following Rotenone Treatment and Complementary Diet with Currants
Prefrontal cortices: The rat medial prefrontal cortex (mPFC), located at anteroposterior (AP) +4.68 to +3.50 mm from bregma, mediolateral (ML) ± 0.7 mm from midline and dorsoventral (DV) −3.5 mm from dura, is also called the prelimbic cortex (PrL). The mPFC contains layer I, layer II/III (associative layers) and layer V/VI (output layers). mPFC layers II/III 5-HT + cell density showed a significant simple main effect of rotenone treatment (F(1.23) = 18.159, p < 0.001), but no significant currant consumption (F(1.23) = 2.380, p = 0.139) nor interaction (F(1.23) = 0.177, p = 0.678) effects were indicated. Specifically, a reduction in 5-HT + cells in mPFC layers II/III of the rotenone-treated (p = 0.014) and rotenone-currant (p = 0.011) groups was observed compared to the equivalent controls ( Figure 2B and Supplementary Figure S1). In the output layer V of mPFC, two-way ANOVA revealed a significant interaction of rotenone treatment × currant consumption (F(1.23) = 10.955, p = 0.003), with significant simple main effect of rotenone treatment (F(1.23) = 6.377, p = 0.020) but there was no difference between the groups consuming currants (F(1.23) = 0.174, p = 0.681). Rotenone treatment significantly decreased layer V 5-HT + cell density (p = 0.004) compared to the control group ( Figure 2C and Supplementary Figure S1). Subsequently, we examined the effects of rotenone and black Corinthian currant consumption on 5-HT + cell density in rat ventral and lateral orbitofrontal cortexes (vOFC, Subsequently, we examined the effects of rotenone and black Corinthian currant consumption on 5-HT + cell density in rat ventral and lateral orbitofrontal cortexes (vOFC, lOFC), located at anteroposterior (AP) +4.68 to +3.50 mm from bregma. No significant interaction between rotenone treatment and supplementary nutrition with currants in layers II/III of vOFC (F(1.23) = 0.120, p = 0.732) and lOFC (F(1.23) = 0.365, p = 0.553) was determined. However, rotenone treatment resulted in a significant simple main effect in layers II/III of vOFC (F(1.23) = 167.546, p < 0.001) ( Figure 2D and Supplementary Figure S2 Figure 3E). Decreased 5-HT + cell densities were observed in the rotenone group without currant intake compared to their controls (p < 0.001). A slight but significant recovery of 5-HT + cells was observed in the BLA of the rotenone-treated group who consumed currants compared with the rotenone group consuming conventional food (p = 0.002).  Next, we questioned whether the BDNF neurotrophic factor is colocalized in SERT expressing cells since it has been suggested to have a role in the pathogenesis of neurodegenerative conditions and mood-related behaviors. Interestingly, a population of hippocampal SERT immunopositive neurons ( Figure 5) also expressed BDNF, highlighting its potential to influence the serotonergic function.  Amygdaloid Complex: SERT + cell density in BLA showed a significant interaction of rotenone treatment × currant consumption (F(1.23) = 8.738, p = 0.008) and significant simple main effect of currant consumption (F(1.23) = 32.565, p < 0.001), but no significant effect of rotenone treatment (F(1.23) = 0.015, p = 0.903). Further analysis with the independent T-test revealed a significant increase in the control group consuming currants compared to control group (p < 0.001) ( Figure 4K).  Figure 6G). Similarly, CA3 β 2 -AR immunodensity showed no statistically significant interaction (F(1.23) = 1.122, p = 0.302) as well as for the simple main effect of currant intake (F(1.23) = 3.071, p = 0.095). Notably, twoway ANOVA indicated that rotenone treatment caused a significant simple main effect (F(1.23) = 37.944, p < 0.001) and particularly a reduction in β 2 -AR immunodensity of the rotenone-treated groups with (p < 0.001) or without (p = 0.005) a currant diet compared to their matching controls ( Figure 6I). In CA2, β 2 -AR + cell density showed a significant simple main effect of rotenone treatment (F(1.23) = 8.558, p = 0.008), but no significant currant intake (F(1.23) = 0.336, p = 0.568) or interaction (F(1.23) = 3.638, p = 0.071) effects. Further analysis with the independent T-test revealed a significant decrease in β 2 -AR immunodensity of rotenone-treated rats without a currant diet (p = 0.005) compared to their controls ( Figure 6H).  Amygdaloid Complex: Two-way ANOVA indicated a significant interaction between the effects of rotenone treatment and currant consumption (F(1.23) = 10.651, p = 0.004). Similarly, the simple main effect of currant consumption (F(1.23) = 19.701, p < 0.001) was significant but not of rotenone treatment (F(1.23) = 2.062, p = 0.166). A significant increase in β 2 -AR + cells was observed in the rotenone group (p = 0.017) compared to controls. Decreased β 2 -AR + cell density was observed in the BLA of the rotenone-currant group compared with the rotenone group (p = 0.002) ( Figure 6J).

Discussion
Induction of PD models using neurotoxins is a valuable tool for investigating the molecular basis of the disease and testing new potential therapeutic strategies. Rotenone treatment results in the loss of TH expressing cells in the SNpc, an underlying factor for the development of motor dysfunction in PD [21,25]. In addition to dopaminergic disruptions, noradrenergic and 5-HT monoamine systems have been suggested to be involved in mechanisms of PD pathogenesis. In line with this, the present study continuing our previous work [21], showed that the dopaminergic nigrostriatal dysfunction in the rat rotenone model was accompanied with an anxiety-like phenotype, associated with basolateral amygdala, hippocampus and medial and orbitofrontal prefrontal cortices 5-HT and noradrenergic neurotransmission. Importantly, a partial recovery of these behavioral and monoaminergic dysfunctions was evident following 38 days of black Corinthian currant consumption, a food source rich in polyphenols. Further identification of monoaminergic alterations and their modulation by dietary natural polyphenols could contribute to our understanding of the manifestation of anxiety/depression in PD, and possibly provide novel complementary intervention approaches.
Our previous study showed that rotenone-treated rats exhibited impaired locomotor activity with reduced velocity, distance traveled and increased immobility time in the OFT. Moreover, it has been documented that these motor deficits were accompanied by significant reductions in TH expressing neurons in both subdivisions of SNpc (lateral and medial) and significant decreases in TH immunodensities (ROD) and TH protein levels in the striatum [21] that includes the SNpc dopaminergic terminals. In addition to motor impairment, rotenone-treated rats exhibited anxiety-like behavior in OFT and EPM. Thigmotaxis [26] and anxiogenic-like effects [27] have been previously validated following 6-OHDA lesions in rats, but not in mice MPTP lesions [28]. The present data are in line with the frequent comorbidity of anxiety/depression observed in PD patients [29], supporting that the manifestation of anxiety can be successfully modeled in the rotenone PD rat model.
Monoaminergic systems have a crucial modulatory role in the organization of corticolimbic circuits of the PFC, hippocampus and amygdala, which are known to process goal-directed behavior, emotions and cognitive information [30,31] and have been suggested to contribute to the anxiety accompanying PD [9]. In accordance, noradrenergic and serotonergic transmission is modulated by the dopaminergic deficiency in PD motor pathology [6]. Indeed, serotonergic and noradrenergic signaling are well associated with emotional responses, especially those of fear and anxiety [32]. The present data clearly showed a rotenone-induced decreased serotonergic immunoreactivity in the corticolimbic circuit, in agreement with previous reports on the degeneration of the 5-HT transmission system [33] and decreased serotonin concentrations in the PFC in PD [34], as well as with evidence that selective serotonin reuptake inhibitors can help the depressive symptomatology in PD [35]. Indeed, increased anxiety levels were accompanied by significant decreases in 5-HT + neurons in medial PFC, ventral and lateral OFC superficial (II-III) and deep (V) layers, CA1 and CA3 hippocampal regions and BLA. In support, it has been found that vesicular monoamine transporter-deficient mice with significant reductions in NE and 5-HT levels in the striatum, hippocampus and cortex exhibited anxiety-like symptoms associated with PD [36].
Rodent prefrontal intermediate II/III and deep cell layers V/VI [37] consist of~80% glutamatergic pyramidal projection neurons and~20% GABAergic local interneurons. Serotonin and adrenergic receptors are mainly expressed in the pyramidal neurons of intermediate and deep layers of mPFC, modulating cortico-thalamic pathways [38]. Although we have not precisely documented the neuronal type of 5-HT, SERT and β 2 -ARs expressing cells in layers II/III and V, their size and morphology clearly suggest that they represent pyramidal cells. However, further studies are needed to determine whether serotonin and β 2 -ARs are co-localized, as suggested for α1-adrenoceptors and 5-HT2-R [39].
In the present study, decreased expression of SERT in the hippocampal regions and superficial layers of vOFC of rotenone-treated rats was coexistent with decreases in 5-HT + cell densities, possibly representing a compensating mechanism to counteract decreased 5-HT levels. That is, SERT down-regulation could support limited 5-HT uptake, resulting in higher synaptic 5-HT levels, while decreasing cytoplasmatic levels. In agreement, 5-HT and SERT levels in striatum and PFC have been shown to decrease [40]. In addition, neuroimaging studies have shown a neocortical reduction in SERT density in animal models [41,42] and SERT down-regulation in the frontal cortices of patients with early PD without pharmacological treatment [43]. In contrast to the hippocampus and vOFC, decreased 5-HT immunoreactivity in BLA was accompanied by an increased SERT immunoreactivity, possibly leading to excessive clearance of synaptic serotonin further adding to the reduced levels of 5-HT. This could contribute to an exaggerated response of the fear circuit, resulting in anxiety-like behavior.
Interestingly, preclinical studies have shown correlations between depressive behavior and decreases in the hippocampal BDNF levels, as well as enhanced expression of BDNF following antidepressant treatment [44]. Indeed, double immunofluorescence of BDNF with SERT indicated the localization of the neurotrophic factor within a population of hippocampal serotoninergic cells. In vivo studies support that BDNF has a neuroprotective role in serotonergic neurons, for example, BDNF increased axon density in 5-HT neurotoxin lesioned rats [45]. Importantly, BDNF decreases in the dopaminergic nigrostriatal pathway were suggested to participate in the motor deficits of a rat rotenone PD-like model [21]. Overall, our data support that serotonergic deficits within the corticolimbic nodes may represent an underlying mechanism of the anxiety-like phenotype; however, further studies are necessary to determine the exact region-specific role of BDNF in animal PD models.
In addition, the PFC of rotenone-treated rats showed complex alterations in the expression of β 2 -ARs. Specifically, β 2 -AR expressing cells were decreased in vOFC, lOFC and hippocampal CA2 and CA3 in rotenone-treated rats, favoring the presence of a regulatory mechanism counteracting a possible increased noradrenaline release. These results are consistent with previous evidence demonstrating that elevated stress and norepinephrine levels are associated with decreased β 2 -AR expression in the hippocampus [46]. In contrast, β 2 -AR immunoreactivity was increased in BLA, probably reflecting the modulatory role of β 2 -ARs in amygdala, mediating the anxiety responses by increased amygdala activity in PD. A previous study, evaluating the monoaminergic dysregulation in corticolimbic domains of macaques, showed MPTP-induced NA depletion in the amygdala [47]. In agreement, reduction in noradrenaline concentration in the CSF of PD patients is significantly correlated with parkinsonian symptoms [48]. These data may indicate the significance of BLA region-specific β 2 noradrenergic receptor up-regulation to the anxiety phenotype, possibly contributing to the decreased serotonergic neurotransmission in the rotenone PD model. Currant supplementation, possibly due to its high phenolic content, reduced thigmotaxic behavior and partly improved the anxiety-like behavior induced by rotenone. Indeed, quantitatively determined phenolic content of Corinthian currants showed higher concentrations of isoquercetin, quercetin, kaempferol, resveratrol and rutin, as well as cinnamic and benzoic acid derivatives. Most of these were detected in the striatum, mesencephalon and hippocampus of rats fed a 3% Corinthian currant supplemented diet for 38 days [15,21]. In agreement with this, quercetin post-treatment of 6-OHDA injection resulted in a decrease in the apomorphine-induced rotational behavior [49]. Similarly, quercetin pre-treatment resulted in anti-inflammatory, antioxidant and neuroprotective actions, together with an improvement in motor performance [50,51]. In addition, resveratrol evoked antidepressant-like effects in chronic unpredictable mild stress model rats [52].
Importantly, parallel to the behavioral effects, currant supplementation significantly attenuated rotenone-induced BLA β 2 -AR increases and partly recovered serotonin cell density in deep layers of mPFC, vOFC, lOFC and BLA, possibly mediating anxiolytic-like effects. In agreement, using the tail-suspension test, a polyphenol rich intake has been previously established to exert anxiolytic-like effects in mice, possibly by increasing the availability of 5-HT and NA in the synaptic cleft [53]. Altogether, our study provides clear evidence of the beneficial effects of black Corinthian currants on the anxiety phenotype of rotenone-treated rats, possibly by rescuing 5-HT in corticolimbic areas.

Ethics Statement
The study was performed in accordance with the EU Directive 2010/63/EU for laboratory animal care and use and was approved by the Ethics committee of Patras University and by the Veterinary Administration of the Prefecture of Achaia, Greece (Protocol number: 187526/625/26-06-2018). All animal experiments were conducted and reported in accordance with ARRIVE guidelines and efforts were made to minimize animal suffering and to reduce the number of animals used.

Animals
Forty-eight adult male Wistar rats, weighing 250-300 g (postnatal days 60 to 70) were used. The rats were kept under standard conditions of temperature (22 ± 2 • C) and relative humidity (55 ± 5%) with a 12-light/12-dark cycle. Rats were handled for a 10-day period to be familiarized with the researchers.

Experimental Design
Rotenone (Sigma, St. Louis, MO, USA) was suspended in vehicle solution containing 1% dimethylsulfoxide (Sigma, St. Louis, MO, USA) in sunflower oil and injected subcutaneously (s.c.) in a volume of 2.5 mg/kg of body weight daily for 28 days [54]. Rotenone was vortexed thoroughly just before injection to ensure a uniform suspension. Currant supplementation was provided as independent food and the determination of daily consumption was based on previous evidence [21]. Forty-eight rats were randomly divided into 4 groups, each containing 12 rats as follows: Control (CTR): Vehicle-injected rats (1 mL/kg/day, s.c.) containing 1% dimethylsulfoxide in sunflower oil for 28 days, following 10 days handling.

Behavioral Testing
Rats were tested in a battery of tasks to determine exploratory and anxiety-like behavior. Prior to behavioral tests, the subjects were allowed to adjust to the new conditions of the testing room for 2 h. Behavior was recorded using a video-tracking system and quantified with the Noldus Ethovision XT7 software. The experimental protocol is shown in Figure 8.

Behavioral Testing
Rats were tested in a battery of tasks to determine exploratory and anxiety-like behavior. Prior to behavioral tests, the subjects were allowed to adjust to the new conditions of the testing room for 2 h. Behavior was recorded using a video-tracking system and quantified with the Noldus Ethovision XT7 software. The experimental protocol is shown in Figure 8.

Open Field
The OFT was used to estimate rat thigmotaxic behavior, i.e., the tendency to stay close to the wall. Rats were placed in a clear Plexiglas open field (100 × 100 × 50 cm) and a

Open Field
The OFT was used to estimate rat thigmotaxic behavior, i.e., the tendency to stay close to the wall. Rats were placed in a clear Plexiglas open field (100 × 100 × 50 cm) and a digital video camera (SONY/HDR-CX625) was installed above the apparatus. On days 10 (2 h after the first injection), 24 and 38 rats were individually placed in the center of the open field apparatus and allowed to explore the field for 10 min.

Elevated Plus Maze
The EPM test consisted of two opposite open (50 cm long × 10 cm wide) and two enclosed arms (50 × 10 × 40 cm) which emerged from a central platform (10 × 10 cm) elevated 50 cm above floor level, based on a design validated by Lister [55].
At the end of the experiment, 1 h after the OFT, each rat was placed in the center of the apparatus facing a closed arm and allowed to explore the maze for 5 min. The time spent on each arm and the latency to the first entry in open arms were recorded. The anxiety index, an index that integrates the EPM behavioral measures, was calculated as follows: anxiety index = 1 − [(Open arm time/Test duration) + (Open arms entries/Total number of entries)/2]. The anxiety index values range from 0 to 1 where an increase in the index expresses increased anxiety-like behavior [56]. The surface of the EPM was cleaned with 70% alcohol before each test.
Immunofluorescence experiments were performed to determine the expression pattern of 5-HT expressing cells and fibers. Briefly, following blocking, sections were incubated overnight at 4 • C with rabbit anti-5-HT (1:400). After washing with PBS three times, the sections were incubated for 2.5 h at room temperature with Alexa-488 conjugated donkey anti-rabbit IgG (1:400, Molecular Probes, Leiden, the Netherlands) in 0.5% PBS-T. Slices were washed five times in PBS for 10 min each and were counterstained with 4 ,6-diamidino-2-phenylindole (DAPI, Molecular Probes, Leiden, the Netherlands).
Double immunofluorescence experiments were performed to determine the co-localization of BDNF on SERT expressing cells and fibers. Briefly, following blocking, sections were incubated with a mixture of primary antibodies diluted in blocking solution for 40 h at 6-7 • C. Antibodies used included monoclonal mouse anti-SERT (1:50) and polyclonal rabbit anti-BDNF (1:300, Novus Biologicals, Englewood, CO, USA). Then, sections were washed in PBS three times, followed by incubation with designated secondary antibodies (Alexa-555 conjugated donkey anti-mouse IgG and Alexa-488 conjugated donkey anti-rabbit IgG (1:400), Molecular Probes, Leiden, the Netherlands) in 0.5% PBS-T for 2.5 h. Slices were washed five times in PBS for 10 min each and coverslipped with fluorescent hard medium (H-1400, Vector Laboratories, Newark, CA, USA).
Details on the antibodies used are shown in Supplementary Table S1.

Quantifications and Photomicrograph Production
Single immunofluorescent images were captured using a colored digital camera CFW-1308C (Scion Corp., Chicago, IL, USA) attached to a Nikon Eclipse E800 optical and fluorescent microscope (Nikon, Tokyo, Japan) and connected to a PC. Double immunofluorescence images were captured using a Leica SP8 confocal microscope. Microscopic images from immunohistochemistry experiments were captured using the Nikon/Optiphot 2 microscope connected to a PC via a color CCD SONY camera (DXC-950P) at X100 and X400 magnification. The immunostained cell bodies for either 5-HT, SERT or β2-ARs were analyzed manually in higher magnification images of PFC, hippocampus and basolateral amygdala. Specifically, cell densities were determined within orbitofrontal and medial prefrontal cortical layers II/III and V. Positive neuronal cell bodies were quantified in three sections (distances of 100 µm) per region per animal, using a graduated frame of 100 µm 2 (×3 graduated frames per section). The nomenclature used was based on the topological rat brain atlas of Paxinos and Watson [24].

Statistical Analysis
The statistical program SPSS (SPSS Statistics 27.0) was employed throughout. Statistical analysis for OFT was evaluated using two-way repeated-measures ANOVA followed by an independent t-Test. For EPM behavioral tests and neurochemical studies, the analysis was performed using a two-way analysis of variance (ANOVA) followed by an independent t-Test. A probability level of 5% (p < 0.05) was considered statistically significant. Data were expressed as mean ± standard error of measurement (SEM) and graphs were constructed in GraphPad Prism 6.0 (GraphPad Software Inc., San Diego, CA, USA). Correlation analysis was evaluated by Pearson product-moment correlation analysis in SPSS and visualized using Origin 2022 (OriginLab Corporation, Northampton, MA, USA).

Conclusions
Rotenone-treated rats exhibited increased anxiety levels, in agreement with the depression and anxiety symptoms in PD patients. Our results revealed complex changes in adrenergic and serotonergic transmissions across the corticolimbic circuit, accompanying the behavioral phenotype. Supplementation with black Corinthian currants, a dried fruit rich in polyphenols, attenuated rotenone-induced anxiety-like behavior and partly reversed the rotenone-induced alterations in the monoaminergic markers studied. Taken all together, the regulation of 5-HT, SERT and β 2 -ARs expression in PFC and BLA may underly the alleviated rotenone-induced anxiety-like behaviors caused by currant consumption. Future research is needed to better understand the specific role of serotonergic transmission in PFC and BLA in anxiety and to determine the underlying mechanisms mediating the polyphenols' neuroprotective actions in serotonergic transmission in PD.

Institutional Review Board Statement:
The study was performed in accordance with the EU Directive 2010/63/EU for laboratory animal care and use and was approved by the Ethics committee of Patras University and by the Veterinary Administration of the Prefecture of Achaia, Greece (Protocol number: 187526/625/26-06-2018). All animal experiments were conducted and reported in accordance with ARRIVE guidelines and efforts were made to minimize animal suffering and to reduce the number of animals used.

Data Availability Statement:
The raw data supporting the data presented in this study are readily available on request from the corresponding author.

Conflicts of Interest:
The authors have no conflict of interest to declare.