Comparison of the Biochemical Properties and Roles in the Xyloglucan-Rich Biomass Degradation of a GH74 Xyloglucanase and Its CBM-Deleted Variant from Thielavia terrestris

Xyloglucan is closely associated with cellulose and still retained with some modification in pretreated lignocellulose; however, its influence on lignocellulose biodegradation is less understood. TtGH74 from Thielavia terrestris displayed much higher catalytic activity than previously characterized fungal GH74 xyloglucanases. The carbohydrate-binding module 1 (CBM1) deleted variant (TtGH74ΔCBM) had the same optimum temperature and pH but an elevated thermostability. TtGH74 displayed a high binding affinity on xyloglucan and cellulose, while TtGH74ΔCBM completely lost the adsorption capability on cellulose. Their hydrolysis action alone or in combination with other glycoside hydrolases on the free xyloglucan, xyloglucan-coated phosphoric acid-swollen cellulose or pretreated corn bran and apple pomace was compared. CBM1 might not be essential for the hydrolysis of free xyloglucan but still effective for the associated xyloglucan to an extent. TtGH74 alone or synergistically acting with the CBH1/EG1 mixture was more effective in the hydrolysis of xyloglucan in corn bran, while TtGH74ΔCBM showed relatively higher catalytic activity on apple pomace, indicating that the role and significance of CBM1 are substrate-specific. The degrees of synergy for TtGH74 or TtGH74ΔCBM with the CBH1/EG1 mixture reached 1.22–2.02. The addition of GH10 xylanase in TtGH74 or the TtGH74ΔCBM/CBH1/EG1 mixture further improved the overall hydrolysis efficiency, and the degrees of synergy were up to 1.50–2.16.

Hemicelluloses are closely associated with the cellulose fibrils and lignin, which limits the accessibility of cellulose to cellulase, hence, greatly hindering the hydrolysis efficiency of cellulase. Hemicelluloses can be divided into four groups, xylan, mannan, xyloglucan and mixed linkage β-glucan [5]. Among them, xyloglucan (XG) is a major complex polysaccharide in the primary cell walls of most higher plants, and it is closely associated with cellulose and covers part of the cellulose surface [6,7].
In addition, XG not only has hydrogen bonds with cellulose but also has ester bonds to the-COOH groups of pectin and benzyl-sugar ether bonds to ferulic acid [7]. XG has a pretreated biomass (including corn stover, corn fiber, sweet sorghum bagasse, barley straw, reed canary grass and willow) could be improved [29,30]. However, compared to the extensive studies of the influence of xylan and mannan in the lignocellulose hydrolysis [31][32][33][34], the effects of XG on the overall lignocellulose hydrolysis efficiency is still less understood, and the role of xyloglucanases needs to be elucidated in depth.
Thielavia terrestris is a thermophilic filamentous fungus and can grow normally at 40-50 • C. It contains a variety of heat-resistant cellulose/hemicellulose hydrolases and can hydrolyze a variety of major polysaccharides in biomass [35]. This strain is a potential source of enzymes with scientific and commercial interests [36]. In this study, one GH74 xyloglucanase from T. terrestris (TtGH74) and its CBM1-deleted variant (TtGH74∆CBM) are expressed in Pichia pastoris, and their characteristics are comprehensively compared.
In addition, their hydrolytic action alone or in combination with cellulase and xylanase on free-XG, xyloglucan-coated phosphoric acid-swollen cellulose (PASC) and different pretreated natural xyloglucan-rich biomasses (corn bran and apple pomace) were also investigated with the aims to deepen our understanding of the effects of the associated XG on the overall hydrolysis efficiency and the role of GH74 xyloglucanases in the degradation of XG-rich biomasses.

Expression and Purification of TtGH74 and TtGH74∆CBM
The structural diagram of TtGH74 and TtGH74∆CBM is shown in Figure 1a. After codon optimization, the codon adaptation index of the TtGH74 was 0.98, and the percentage of GC content was 40.93%. The codon-optimized genes encoding GH74 xyloglucanase from T. terrestris (TtGH74) and CBM-deleted variant TtGH74∆CBM were successfully expressed in P. pastoris KM71H. The purified enzymes were analyzed by SDS-PAGE, and the molecular masses of TtGH74 and TtGH74∆CBM were about 90 and 85 kDa, respectively. After digestion by N-glycosidase Endo H, SDS-PAGE analysis showed that their molecular masses decreased closely to theoretical values of 87.89 and 83.09 kDa, respectively (Figure 1b). cellulase and xylanase for the efficient hydrolysis of lignocellulose biomass. Recently, it was reported that the deletion of xyloglucanase gene (cel74a) significantly affected the expression of holocellulolytic genes and reduced the degradation efficiency of sugarcane bagasse [28].
Previous experiments showed that added the so-called accessory enzyme xyloglucanase to the cellulase mixture, the hydrolytic performance of the cellulase mixture on pretreated biomass (including corn stover, corn fiber, sweet sorghum bagasse, barley straw, reed canary grass and willow) could be improved [29,30]. However, compared to the extensive studies of the influence of xylan and mannan in the lignocellulose hydrolysis [31][32][33][34], the effects of XG on the overall lignocellulose hydrolysis efficiency is still less understood, and the role of xyloglucanases needs to be elucidated in depth.
Thielavia terrestris is a thermophilic filamentous fungus and can grow normally at 40-50 °C. It contains a variety of heat-resistant cellulose/hemicellulose hydrolases and can hydrolyze a variety of major polysaccharides in biomass [35]. This strain is a potential source of enzymes with scientific and commercial interests [36]. In this study, one GH74 xyloglucanase from T. terrestris (TtGH74) and its CBM1-deleted variant (TtGH74ΔCBM) are expressed in Pichia pastoris, and their characteristics are comprehensively compared.
In addition, their hydrolytic action alone or in combination with cellulase and xylanase on free-XG, xyloglucan-coated phosphoric acid-swollen cellulose (PASC) and different pretreated natural xyloglucan-rich biomasses (corn bran and apple pomace) were also investigated with the aims to deepen our understanding of the effects of the associated XG on the overall hydrolysis efficiency and the role of GH74 xyloglucanases in the degradation of XG-rich biomasses.

Expression and Purification of TtGH74 and TtGH74ΔCBM
The structural diagram of TtGH74 and TtGH74ΔCBM is shown in Figure 1a. After codon optimization, the codon adaptation index of the TtGH74 was 0.98, and the percentage of GC content was 40.93%. The codon-optimized genes encoding GH74 xyloglucanase from T. terrestris (TtGH74) and CBM-deleted variant TtGH74ΔCBM were successfully expressed in P. pastoris KM71H. The purified enzymes were analyzed by SDS-PAGE, and the molecular masses of TtGH74 and TtGH74ΔCBM were about 90 and 85 kDa, respectively. After digestion by N-glycosidase Endo H, SDS-PAGE analysis showed that their molecular masses decreased closely to theoretical values of 87.89 and 83.09 kDa, respectively ( Figure 1b).  The analysis of the amino acid sequences of TtGH74 and TtGH74∆CBM by NetNGlyc 1.0 Server (https://services.healthtech.dtu.dk/service.php?NetNGlyc-1.0; accessed on 9 March 2022) confirmed that they have three putative N-glycosylation sites (N212, N325 and N409), thus, indicating that the recombinant TtGH74 and TtGH74∆CBM could partially be N-glycosylated.
The protein expression level of TtGH74 was only 60% of that of TtGH74∆CBM, therefore, the expression conditions of TtGH74 were optimized. The methanol-regulated pro-moter of the alcohol oxidase 1 gene (AOX1) is the key to the high expression level of recombinant protein in P. pastoris [37], which was significantly influenced by the methanol concentration. In addition, the expression level was also affected by the medium's pH, induction temperature and time. Under the optimized condition, the enzyme activity of TtGH74 secreted in the supernatant reached 94.65 U/mL after 5 days of induction with 1.5% methanol at pH 6.0 and 28 • C ( Figure S1).

Properties of Recombinant TtGH74 and TtGH74∆CBM from P. pastoris
Among all tested substrates, TtGH74 showed ultra-high activity against tamarind seed XG. TtGH74 also showed activities toward barley β-glucan, konjac glucomannan, lichenan and PASC; however, their corresponding activities were only approximately 5% and 1% of that on XG. No activities were detected towards other polysaccharides, such as laminarin, starch, xylan, pectin and chitin. (Table 1). TtGH74 and TtGH74∆CBM displayed the maximum activity toward tamarind seed XG at 75 • C and pH 5.5 (Figure 2a,b). However, the enzymes were unstable at 70 • C. After pre-incubation at 70 • C for 30 min, they completely lost their activities ( Figure 2c). This result indicated that the enzymes had more thermostability in the presence of the substrate, which may be attributed to the stabilizing effects of the substrate [38]. After pre-incubation at 65 • C for 12 h, both enzymes lost their activities completely. Notably, the deletion of CBM1 significantly improved the thermostability of TtGH74∆CBM when the pre-incubation of enzymes was at temperatures below 65 • C.
With the pre-incubation of enzymes at 60 • C to 55 • C for 12 h, the residual activity of TtGH74∆CBM was 6% to 11% higher than that of TtGH74. With the pre-incubation of enzymes at 50 • C for 12 h, the activity of TtGH74 remained above 77%, while the activity of TtGH74∆CBM remained above 89% (Figure 2c). The data differences regarding TtGH74 and TtGH74∆CBM thermostability were statistically significant (p < 0.05). Conversely, the thermostability of some glycoside hydrolases from fungi was decreased after the removal of CBM1 [39,40]. The inconsistent observation about CBM1 suggests that its role in thermostability might be related to the kinds of enzymes. The enzymes were stable in a pH range from 3 to 8 (Figure 2d). of CBM1 [39,40]. The inconsistent observation about CBM1 suggests that its role in thermostability might be related to the kinds of enzymes. The enzymes were stable in a pH range from 3 to 8 (Figure 2d). The effects of metal ions (1 and 5 mM) on the enzyme activities was investigated, and the results are shown in Figure S2. The activities of TtGH74 and TtGH74ΔCBM were increased by 2-25% in the presence of 5 mM K + , Ca 2+ , Ba 2+ and Ni 2+ ions. At the same time, the enzyme activities were decreased by 5-75% in the presence of (1 and 5 mM) EDTA, NH4 + , Li + , Mg 2+ , Pb 2+ , Zn 2+ , Cu 2+ , Mn 2+ , Co 2+ , Fe 3+ and Al 3+ . We found that 5 mM Pb 2+ and Fe 3+ could dramatically reduce the activities of TtGH74 and TtGH74ΔCBM by 90%.
The kinetic values were determined in the concentration ranges of 0.2-6.0 mg/mL of tamarind seed XG (Table 2). It can be seen from Table 2 that the catalytic activity of TtGH74 is much higher than the previously characterized GH74 xyloglucanases. TtGH74ΔCBM has a slightly smaller Km and Kcat value than TtGH74 (Table 2). Research reported that the Kcat of GH74 xyloglucanase from Phanerochaete chrysosporium was increased slightly after the deletion of CBM1 [41]. These results suggested that CBM1 in GH74 xyloglucanases slightly affected its catalytic activity for the soluble XG. The effects of metal ions (1 and 5 mM) on the enzyme activities was investigated, and the results are shown in Figure S2. The activities of TtGH74 and TtGH74∆CBM were increased by 2-25% in the presence of 5 mM K + , Ca 2+ , Ba 2+ and Ni 2+ ions. At the same time, the enzyme activities were decreased by 5-75% in the presence of (1 and 5 mM) EDTA, NH 4 + , Li + , Mg 2+ , Pb 2+ , Zn 2+ , Cu 2+ , Mn 2+ , Co 2+ , Fe 3+ and Al 3+ . We found that 5 mM Pb 2+ and Fe 3+ could dramatically reduce the activities of TtGH74 and TtGH74∆CBM by 90%.
The kinetic values were determined in the concentration ranges of 0.2-6.0 mg/mL of tamarind seed XG (Table 2). It can be seen from Table 2 that the catalytic activity of TtGH74 is much higher than the previously characterized GH74 xyloglucanases. TtGH74∆CBM has a slightly smaller K m and K cat value than TtGH74 (Table 2). Research reported that the K cat of GH74 xyloglucanase from Phanerochaete chrysosporium was increased slightly after the deletion of CBM1 [41]. These results suggested that CBM1 in GH74 xyloglucanases slightly affected its catalytic activity for the soluble XG. The end-products generated from the tamarind seed XG after TtGH74 hydrolysis were identified using matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). A series of sodium adducts of the hydrolyzed products were detected ( Figure 3). The m/z values of the major peaks were 1085, 1247 and 1409 corresponding to molecular masses of the reduced oligosaccharide ions [XXXG + Na] + , [XXLG + Na] + and [XLLG + Na] + , respectively.

Adsorption of TtGH74 and TtGH74ΔCBM on Different Substrates
The binding affinity of TtGH74 and TtGH74ΔCBM on different substrates (10 mg in 0.5 mL) was compared by measuring the unbound protein in the supernatant. The results are shown in Figure 4a. All TtGH74 protein bound onto PASC, while only approximately 35% and 23% of TtGH74 bound onto Avicel and Whatman filter paper, respectively. For pretreated lignocellulosic substrates, the amounts of bound protein in ascending order are 15%, 20% and 30% for deep eutectic solvents (DES)-pretreated corn directly proportional to the galactose content in the substrates (Table 3).
Without CBM1, the adsorption capacity of TtGH74ΔCBM for insoluble carbohydrates is almost completely lost. We further measured the adsorption of TtGH74 under the condition of low cellulose content (1 mg in 0.5 mL) with additional different proportions of XG (Figure 4b). Due to the relative excess amount of enzyme, the unbound protein was detected in supernatant; however, with the increase of XG proportion, the amount of the unbound protein gradually decreased, which implied that the TtGH74 could bind onto XG. To further verify the adsorption of CBM1 on XG, we compared the mobility of the CBM-deleted variant TtGH74ΔCBM with the TtGH74 in xyloglucan-containing native gels.
We observed that the mobility of TtGH74ΔCBM increased significantly compared to TtGH74, which further clarified the binding affinity of CBM1 in TtGH74 onto XG ( Figure  4c,d). The binding affinity of CBM1 on cellulose and the role of CBM1 in cellulase functionality were well demonstrated in the literature [47,48]. CBM1 is type A CBM and was previously recognized as a cellulose-binding domain due to the first discovery in fungal cellulases [49]. However, the effects of CBM1 on XG binding and the activity of fungal Apart from the main XG building blocks, various low molecular products were generated during the hydrolysis and the m/z values detected by mass spectrometry may potentially correspond to XX, XXG, GXX, XGX, XL, XLG, GXL, LL, LG, XXL, LLG, GXXXG, GXLLG and XLLGX oligosaccharides, respectively; however, no X, G and L were detected. Although TtGH74 randomly hydrolyzed the XG backbone, it did not cleave at the reducing end side of L units in XLXG and XLLG [46] as shown in the schematic diagram.
TtGH74 released oligo-xyloglucans, such as XXXG, XXLG and XLLG, which were the typical final products of endo-type xyloglucanases. In addition, the sequence alignments of TtGH74 with other fungal GH74 xyloglucanases ( Figure S3) revealed that TtGH74 has four conserved tryptophan residues that were regarded as the key amino acid residues for the endo-processive activity of GH74 xyloglucanases [17]. Thus, this indicates that TtGH74 is an endo-processive-type xyloglucanase.

Adsorption of TtGH74 and TtGH74∆CBM on Different Substrates
The binding affinity of TtGH74 and TtGH74∆CBM on different substrates (10 mg in 0.5 mL) was compared by measuring the unbound protein in the supernatant. The results are shown in Figure 4a. All TtGH74 protein bound onto PASC, while only approximately 35% and 23% of TtGH74 bound onto Avicel and Whatman filter paper, respectively. For pretreated lignocellulosic substrates, the amounts of bound protein in ascending order are 15%, 20% and 30% for deep eutectic solvents (DES)-pretreated corn directly proportional to the galactose content in the substrates (Table 3).

Hydrolysis Action of TtGH74 and TtGH74ΔCBM on XG and XG-Coated PASC
Hydrolysis action of TtGH74 and TtGH74ΔCBM on free XG was performed in the concentration ranges of 25-600 μg XG (Figure 5a). When the amount of XG was low (less than 250 μg), TtGH74ΔCBM exhibited a slightly lower hydrolysis yield than the intact enzyme due to its low affinity to the substrate. However, as the amount of XG increased, the yield of reducing sugars by TtGH74ΔCBM hydrolysis increased significantly and surpassed the yield by TtGH74 as the content of XG was over 250 µg.  Without CBM1, the adsorption capacity of TtGH74∆CBM for insoluble carbohydrates is almost completely lost. We further measured the adsorption of TtGH74 under the condition of low cellulose content (1 mg in 0.5 mL) with additional different proportions of XG ( Figure 4b). Due to the relative excess amount of enzyme, the unbound protein was detected in supernatant; however, with the increase of XG proportion, the amount of the unbound protein gradually decreased, which implied that the TtGH74 could bind onto XG. To further verify the adsorption of CBM1 on XG, we compared the mobility of the CBM-deleted variant TtGH74∆CBM with the TtGH74 in xyloglucan-containing native gels.
We observed that the mobility of TtGH74∆CBM increased significantly compared to TtGH74, which further clarified the binding affinity of CBM1 in TtGH74 onto XG (Figure 4c,d). The binding affinity of CBM1 on cellulose and the role of CBM1 in cellulase functionality were well demonstrated in the literature [47,48]. CBM1 is type A CBM and was previously recognized as a cellulose-binding domain due to the first discovery in fungal cellulases [49]. However, the effects of CBM1 on XG binding and the activity of fungal GH74 xyloglucanases have been little reported. Our results suggested that CBM1 in TtGH74 displayed a high binding affinity for both cellulose and XG.

Hydrolysis Action of TtGH74 and TtGH74∆CBM on XG and XG-Coated PASC
Hydrolysis action of TtGH74 and TtGH74∆CBM on free XG was performed in the concentration ranges of 25-600 µg XG (Figure 5a). When the amount of XG was low (less than 250 µg), TtGH74∆CBM exhibited a slightly lower hydrolysis yield than the intact enzyme due to its low affinity to the substrate. However, as the amount of XG increased, the yield of reducing sugars by TtGH74∆CBM hydrolysis increased significantly and surpassed the yield by TtGH74 as the content of XG was over 250 µg. XG-coated PASC with different proportions of XG were used as the substrates to investigate the influence of CBM1 in enzyme functionality. XG can coat onto the surface of PASC once mixture, however, the association pattern between XG with PASC relies on the proportion ratio of XG/PASC. The previous research revealed that at a low XG/cellulose concentration ratio (25 μg/mg), all XG is tightly bound to the cellulose surface, and XG is not easy to be hydrolyzed by the enzyme. However, with the increase of XG/cellulose ratio, XG forms accessible "loops" and "tails" on the cellulose surface, and the accessible XG gradually increases to a constant value [50]. The schematic diagram of the association pattern of XG and PASC is shown in Figure 5b. In addition, when the XG/PASC ratio was over 400 μg/mg, free XG could be detected in the liquid fraction ( Figure S4).
When XG-coated PASC was reacted with TtGH74 or TtGH74ΔCBM (Figure 5a), XG was more difficult to be hydrolyzed than free XG by TtGH74 and TtGH74ΔCBM because of the close association of XG with PASC. Similar as free XG, once the proportion of XG increased over 400 μg/mg, TtGH74ΔCBM released more reducing sugars from XG-coated PASC than TtGH74. Since free XG was detected in the supernatant of XG-coated PASC solution with a high XG/cellulose ratio; thus, the higher catalytic performance of TtGH74ΔCBM than TtGH74 in the hydrolysis of XG-coated PASC solution as the proportion of XG over 400 µg/mg might be partially due to the presence of free XG in solution.
However, relatively higher reducing sugars were released from XG-coated PASC by TtGH74 than from TtGH74ΔCBM as the proportional ratio of XG/PASC was below 300 μg/mg. These results indicated that CBM1 might not be essential for the hydrolysis of free XG but was effective for the associated XG to some degree. The coverage of XG on PASC even at low concentrations resulted in a significant decrease in the catalytic activity of endoglucanase 1 (EG1); in comparison, a smaller reduction in cellobiohydrolase 1 (CBH1) XG-coated PASC with different proportions of XG were used as the substrates to investigate the influence of CBM1 in enzyme functionality. XG can coat onto the surface of PASC once mixture, however, the association pattern between XG with PASC relies on the proportion ratio of XG/PASC. The previous research revealed that at a low XG/cellulose concentration ratio (25 µg/mg), all XG is tightly bound to the cellulose surface, and XG is not easy to be hydrolyzed by the enzyme. However, with the increase of XG/cellulose ratio, XG forms accessible "loops" and "tails" on the cellulose surface, and the accessible XG gradually increases to a constant value [50]. The schematic diagram of the association pattern of XG and PASC is shown in Figure 5b. In addition, when the XG/PASC ratio was over 400 µg/mg, free XG could be detected in the liquid fraction ( Figure S4).
When XG-coated PASC was reacted with TtGH74 or TtGH74∆CBM (Figure 5a), XG was more difficult to be hydrolyzed than free XG by TtGH74 and TtGH74∆CBM because of the close association of XG with PASC. Similar as free XG, once the proportion of XG increased over 400 µg/mg, TtGH74∆CBM released more reducing sugars from XGcoated PASC than TtGH74. Since free XG was detected in the supernatant of XG-coated PASC solution with a high XG/cellulose ratio; thus, the higher catalytic performance of TtGH74∆CBM than TtGH74 in the hydrolysis of XG-coated PASC solution as the proportion of XG over 400 µg/mg might be partially due to the presence of free XG in solution.
However, relatively higher reducing sugars were released from XG-coated PASC by TtGH74 than from TtGH74∆CBM as the proportional ratio of XG/PASC was below 300 µg/mg. These results indicated that CBM1 might not be essential for the hydrolysis of free XG but was effective for the associated XG to some degree. The coverage of XG on PASC even at low concentrations resulted in a significant decrease in the catalytic activity of endoglucanase 1 (EG1); in comparison, a smaller reduction in cellobiohydrolase 1 (CBH1) activity was observed (Figure 5a).
These differences might be attributed to the different catalytic patterns between EG1 and CBH1. EG1 is a GH5 endo-glucanase, which randomly attacks on internal sites in the cellulose chain [51], while CBH1 is an exo-cellulase, which processively hydrolyzes the cellulose chain from reducing end [52].

Evaluation of the Presence of XG and Hydrolysis Action of TtGH74 and TtGH74∆CBM on Pretreated Corn Bran and Apple Pomace
To evaluate the presence of XG in solid residues after pretreatment in corn bran and apple pomace, two substrates were pretreated at 90 • C by 1% sulfuric acid or DES at different times (3, 6, 9 and 12 h). The residues were then hydrolyzed by TtGH74 or TtGH74∆CBM. As shown in Figure 6, a large amount of reducing sugars was released from all pretreated residues, indicating that XG was still retained after pretreatment. Interestingly, no matter what kinds of pretreatment methods and times used, TtGH74 produced more reducing sugars than TtGH74∆CBM from pretreated corn bran after four days of reaction, while TtGH74∆CBM had better hydrolysis performance on DES-pretreated apple pomace than TtGH74 (Figure 6).
To evaluate the presence of XG in solid residues after pretreatment in corn bran and apple pomace, two substrates were pretreated at 90 °C by 1% sulfuric acid or DES at different times (3, 6, 9 and 12 h). The residues were then hydrolyzed by TtGH74 or TtGH74ΔCBM. As shown in Figure 6, a large amount of reducing sugars was released from all pretreated residues, indicating that XG was still retained after pretreatment. Interestingly, no matter what kinds of pretreatment methods and times used, TtGH74 produced more reducing sugars than TtGH74ΔCBM from pretreated corn bran after four days of reaction, while TtGH74ΔCBM had better hydrolysis performance on DES-pretreated apple pomace than TtGH74 ( Figure 6).
Finally, corn bran pretreated with sulfuric acid for 6 h, corn bran and apple pomace pretreated with DES for 9 h were used as substrates for further exploration. As shown in Table 3, three pretreated residues differed in chemical compositions due to different sources and pretreatment methods. DES pretreatment was more effective in the removal of lignin and hemicelluloses than sulfuric-acid pretreatment for corn bran (p < 0.01). Thus, in this study, only DES was used to pretreat apple pomace. The DES-pretreated apple pomace had the highest content of galactose among the three substrates (p < 0.01). Galactose is a specific substituent in the side branch of XG.
Galactose may also come from the main chain of residual pectin; however, galactose in the pectin of apple accounts for approximately 1% of total pectin according to the literature [53], and thus the galactose in pretreated apple pomace mainly come from the side chain of XG. Therefore, the higher content of galactose represented the higher amount of XG. This might explain the higher adsorption capacity of TtGH74 on DES-pretreated apple pomace than DES-pretreated and sulfuric-acid-pretreated corn bran (Figure 4a).
This might also explain the much higher reducing sugars released from DES-pretreated apple pomace by either TtGH74 or TtGH74ΔCBM (Figure 6c). The content of lignin in DES-pretreated corn bran is much lower than sulfuric-acid-pretreated corn bran (Table 3). This might contribute to the more reducing sugars generated from DES-pretreated corn bran than sulfuric-acid-pretreated corn bran, although the content of XG is relatively lower in DES-pretreated corn bran.

Figure 6.
TtGH74 and TtGH74ΔCBM enzymatic hydrolysis of sulfuric-acid-pretreated corn bran (a), DES-pretreated corn bran (b) and DES-pretreated apple pomace (c) with different pretreatment times. The product yield was expressed in grams of reducing sugar produced by per kilogram of dry material (Reducing Sugars g/kg Dry Material, RS g/kg DM). The statistical differences between two groups of TtGH74 and TtGH74ΔCBM were determined using Student's t-test analysis. Statistical significance is defined as *p < 0.05 and ** p < 0.01.
The presence of XG in three pretreated substrates was confirmed by detecting the TtGH74 hydrolysis end-products using high performance anion exchange chromatog- Figure 6. TtGH74 and TtGH74∆CBM enzymatic hydrolysis of sulfuric-acid-pretreated corn bran (a), DES-pretreated corn bran (b) and DES-pretreated apple pomace (c) with different pretreatment times. The product yield was expressed in grams of reducing sugar produced by per kilogram of dry material (Reducing Sugars g/kg Dry Material, RS g/kg DM). The statistical differences between two groups of TtGH74 and TtGH74∆CBM were determined using Student's t-test analysis. Statistical significance is defined as *p < 0.05 and ** p < 0.01.
Finally, corn bran pretreated with sulfuric acid for 6 h, corn bran and apple pomace pretreated with DES for 9 h were used as substrates for further exploration. As shown in Table 3, three pretreated residues differed in chemical compositions due to different sources and pretreatment methods. DES pretreatment was more effective in the removal of lignin and hemicelluloses than sulfuric-acid pretreatment for corn bran (p < 0.01). Thus, in this study, only DES was used to pretreat apple pomace. The DES-pretreated apple pomace had the highest content of galactose among the three substrates (p < 0.01). Galactose is a specific substituent in the side branch of XG.
Galactose may also come from the main chain of residual pectin; however, galactose in the pectin of apple accounts for approximately 1% of total pectin according to the literature [53], and thus the galactose in pretreated apple pomace mainly come from the side chain of XG. Therefore, the higher content of galactose represented the higher amount of XG. This might explain the higher adsorption capacity of TtGH74 on DES-pretreated apple pomace than DES-pretreated and sulfuric-acid-pretreated corn bran (Figure 4a). This might also explain the much higher reducing sugars released from DES-pretreated apple pomace by either TtGH74 or TtGH74∆CBM (Figure 6c). The content of lignin in DES-pretreated corn bran is much lower than sulfuric-acid-pretreated corn bran (Table 3). This might contribute to the more reducing sugars generated from DES-pretreated corn bran than sulfuric-acid-pretreated corn bran, although the content of XG is relatively lower in DES-pretreated corn bran.
The presence of XG in three pretreated substrates was confirmed by detecting the TtGH74 hydrolysis end-products using high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), and the formation of oligosaccharides produced by TtGH74 hydrolysis of three pretreated substrates were further confirmed by MALDI-TOF MS.
Comparing Figure 7a,c, the profiles of the peak of oligosaccharides produced by TtGH74 hydrolysis of corn bran with different pretreatment methods were similar in HPAEC-PAD analysis; however, the size of the peaks were different (the special peaks were numbered according to the elution time). For example, the proportion of peak 4 in Figure 7c was significantly higher than that in Figure 7a. However, for different substrates with the same pretreatment method, the profiles of the overall peak were different but the proportion of peak 4 was the same (Figure 7c,e).
It could be inferred that xyloglucan in sulfuric-acid-pretreated corn bran contains more and longer branch chains than that in DES-pretreated corn bran. Like DES pretreated corn bran, more oligosaccharides of XG, XX, XL, XXG and XGX were released from DES-pretreated apple pomace (Figure 7d,f); however, many XLG and GXL were also released from DES-pretreated apple pomace, which was due to the difference between the composition of xyloglucan in apple pomace and corn bran.
In summary, different pretreatment methods might have various fractionalization and modification effects on xyloglucan in lignocellulosic biomass, resulting in the content change and structural modification of xyloglucan in different pretreated lignocellulosic biomasses; therefore, the end-product profiles in hydrolysates are different.
The time courses of enzymatic hydrolysis of three pretreated substrates in different concentrations (from 10 to 80 mg) were performed, and the results are shown in Figure 8. In general, TtGH74 produced more reducing sugars than TtGH74∆CBM from sulfuric-acidpretreated corn bran and DES-pretreated corn bran after four days of hydrolysis; however, the increase ranges varied depending on the concentration of substrates. For the two pretreated corn brans, at low substrate concentration (10 mg), the yields of reducing sugar by TtGH74 were 1.23 (p < 0.05) and 1.39 (p < 0.05)-times higher than TtGH74∆CBM. The yields of reducing sugar by TtGH74 were 1.36 (p < 0.05) and 1.13 (p < 0.05)-times higher than TtGH74∆CBM when the substrate concentration was 20 mg.

Figure 7. HPAEC-PAD (left) and MALDI-TOF MS (right) analysis of end-products generated by
TtGH74 from sulfuric-acid-pretreated corn bran (a,b), DES-pretreated corn bran (c,d) and apple pomace (e,f). The blank in the HPAEC-PAD represents a control experiment without TtGH74.
In summary, different pretreatment methods might have various fractionalization and modification effects on xyloglucan in lignocellulosic biomass, resulting in the content change and structural modification of xyloglucan in different pretreated lignocellulosic biomasses; therefore, the end-product profiles in hydrolysates are different.
The time courses of enzymatic hydrolysis of three pretreated substrates in different concentrations (from 10 to 80 mg) were performed, and the results are shown in Figure 8. In general, TtGH74 produced more reducing sugars than TtGH74ΔCBM from sulfuricacid-pretreated corn bran and DES-pretreated corn bran after four days of hydrolysis; however, the increase ranges varied depending on the concentration of substrates. For the two pretreated corn brans, at low substrate concentration (10 mg), the yields of reducing sugar by TtGH74 were 1.23 (p < 0.05) and 1.39 (p < 0.05)-times higher than TtGH74ΔCBM. The yields of reducing sugar by TtGH74 were 1.36 (p < 0.05) and 1.13 (p < 0.05)-times higher than TtGH74ΔCBM when the substrate concentration was 20 mg. At concentrations of 40-80 mg, the yield of reducing sugar by TtGH74 was about 1.12 (p < 0.05)-times higher than that of TtGH74∆CBM. On the contrary, TtGH74∆CBM produced more reducing sugars than TtGH74 from DES-pretreated apple pomace, no matter what concentration of substrate was used. The yields of reducing sugar by TtGH74∆CBM were 1.68-2.06 (p < 0.03)-times higher than that of TtGH74 at concentrations of 10-80 mg. From the time course experiment, we concluded that the presence of CBM1 is conducive to enzymatic hydrolysis; however, its role and significance are substrate-specific because of the differences in the contents and structure of XG in different biomasses.

Synergistic Action of TtGH74 or TtGH74∆CBM with CBH1/EG1 Mixture and Xylanase on Pretreated Corn Bran and Apple Pomace
As specific xyloglucan degrading enzymes, GH74 xyloglucanases were widely distributed in bacteria and fungi, and they were usually co-expressed with cellulase and xylanase. In view of the presence of XG in pretreated corn bran and apple pomace (Table 3) and its blocking effect on EG1 and CBH1 activity towards XG-coated PASC (Figure 5), it is reasonable to believe that xyloglucanases may be necessary for the efficient enzymatic saccharification of XG-rich biomasses.
Thus, we further investigated the synergy between xyloglucanase with xylanase and cellulase in the hydrolysis of pretreated corn bran and apple pomace. As shown in Figure 9a As shown in Figure 9a,b, for three pretreated substrates, when replacing TtGH74 with TtGH74ΔCBM in the enzymatic hydrolysis, the reducing sugar yields of TtGH74ΔCBM hydrolysis were 9.54, 16.00 and 23.30 (RS g/kg DM) for sulfuric-acid-pretreated corn bran, DES-pretreated corn bran and DES-pretreated apple pomace, respectively. The yields of the combined action of TtGH74ΔCBM with CBH1/EG1 mixture were 121.20, 105.39 and 65.06, and the degrees of synergy were 1.33, 1.22 and 1.38, respectively. Correspondingly, when the enzymes act synergistically, the yields of reducing sugar by the combined action of GH10 xylanase with CBH1/EG1 mixture were 131. 38 19, and the degrees of synergy were 2.16, 1.54 and 1.79 for sulfuric-acid-pretreated corn bran, DES-pretreated corn bran and DES-pretreated apple pomace, respectively.
The above results indicated that either TtGH74 or GH10 xylanase showed a boosting effect on the hydrolysis efficiency of the CBH1/EG1 mixture, in comparison, the synergistic action between TtGH74 and the CBH1/EG1 mixture was significantly higher than that of GH10 xylanase and the CBH1/EG1 mixture. Interestingly, the degrees of synergy between GH10 xylanase and TtGH74 were 0.62, 0.88 and 0.69, respectively, indicating that GH10 xylanase and TtGH74 not only had no synergy but also had an inhibition effect on each other due to the close spatial location of xyloglucan and xylan. However, the quaternary mixture of TtGH74, GH10 xylanase and CBH1/EG1 resulted in more synergistic action than the ternary mixture of TtGH74 with CBH1/EG1.
As shown in Figure 9a,b, for three pretreated substrates, when replacing TtGH74 with TtGH74∆CBM in the enzymatic hydrolysis, the reducing sugar yields of TtGH74∆CBM hydrolysis were 9.54, 16.00 and 23.30 (RS g/kg DM) for sulfuric-acid-pretreated corn bran, DES-pretreated corn bran and DES-pretreated apple pomace, respectively. The yields of the combined action of TtGH74∆CBM with CBH1/EG1 mixture were 121.20, 105.39 and 65.06, and the degrees of synergy were 1.33, 1.22 and 1.38, respectively.
The yields of reducing sugar by the combined action of TtGH74∆CBM with GH10 xylanase and CBH1/EG1 mixture were 195.42, 145.68 and 98.80, and the degrees of synergy reached up to 1.82, 1.50 and 1.88, respectively. Similarly, no synergy but inhibition effect on each other was observed for TtGH74∆CBM and GH10 xylanase, and the degrees of synergy were 0.60, 0.82 and 0.44, respectively.
In contrast, the boosting effects of TtGH74∆CBM was much higher than TtGH74 in terms of the degrees of synergy for pretreated apple pomace (1.24 vs. 1.38 and 1.79 vs. 1.88) (p < 0.05). This is consistent with the bias of their activities when acted on three pretreated substrates alone (Figure 8), i.e., TtGH74 showed higher activity towards pretreated corn bran than TtGH74∆CBM. Conversely, TtGH74∆CBM had much higher activity than TtGH74 towards pretreated apple pomace.
In order to verify whether the above synergistic action was attributed to the presence of XG in the substrates, the associated XG was extracted by strong alkali treatment and XGfree residuals were used as substrates for synergistic experiments. As shown in Figure S5, tiny oligosaccharides could be released from strong alkali-treated residual by TtGH74 hydrolysis based on the analysis of HPAEC-PAD, indicating that most of XG was removed by strong alkali treatment.
In addition, almost no reducing sugar was produced from the strong alkali-treated residual by GH10 xylanase hydrolysis (Figure 9c), indicating that the strong alkali treatment also removed most of the xylan from pretreated lignocelluloses. When XG and xylan-free substrates were hydrolyzed by the mixture of CBH1/EG1 and GH10 xylanase, CBH1/EG1 and TtGH74 and the mixture of TtGH74/CBH1/EG1/GH10 xylanase, no synergistic action was observed.
Their corresponding reducing sugar yields were almost the same as those of the CBH1/EG1 mixture alone, and all degrees of synergy were close to 1 (Figure 9c,d). However, we found that the hydrolysis efficiency of the CBH1/EG1 mixture on the strong alkali treated residues was greatly improved. The reducing sugar yields reached up to 192. 29,114.81 and 93.54 (RS g/kg DM), respectively, which were 136.20%, 63.41% and 290.72% (p < 0.01) higher than the corresponding reducing sugar yields of the CBH1/EG1 mixture on sulfuric-acid-pretreated corn bran, DES-pretreated corn bran and DES-pretreated apple pomace, respectively (Figure 9a). Thus, it further confirmed that the blocks of XG were one of the great significant factors impeding the degradation of xyloglucan-rich lignocellulose.
The structures, activity modes and enzymatic properties of GH74 xyloglucanases have been widely studied in the literature [17,18,[41][42][43][44][45]; however, their roles in lignocellulose biomass degradation have been scarcely reported. Benko [30] et al. studied the contribution of the added GH74 xyloglucanase from Trichoderma reesei (TrGH74) in the cellulase mixture to degradation of steam pretreated willow, barley straw, wheat straw, reed canary grass, corn stover and Solka Floc.They found that the degree of synergy of TrGH74 with the cellulase mixture reached 1.22 for barley straw; however, only 1.07, 1.10 and 1.10 for corn stover, reed canary grass and willow, respectively. No synergistic effect was observed for wheat straw and Solka Floc.
Research suggested that the degree of synergy between xyloglucanase and the cellulase mixture was positively correlated with the ratio of xylose to glucose in the substrates. Overall, our study revealed that the degree of synergy between TtGH74 and the cellulase mixture was much higher for both the pretreated corn bran or apple pomace when compared with the previous reports because of the higher content of XG in these residues.
However, the influence factors involving synergy might be multiple and complicated. Apart from the XG content, the content of lignin and xylan and the structural modification of XG might also have a great influence on the synergy efficiency. The changes of composition and structure of XG in different pretreated residues in turn affected enzyme-substrate interaction. We observed that the degree of synergy between TtGH74 and the cellulase mixture on sulfuric-acid-pretreated corn bran was much higher than that on DES-pretreated corn bran; however, they became close since the degrees of synergy were reduced by 34.16% and 10.29% on sulfuric-acid-pretreated and DES-pretreated corn bran when replacing with TtGH74∆CBM, respectively.
In view of the more and longer branched chains of XG in sulfuric-acid-pretreated compared with DES-pretreated corn bran (Figure 7b,d), it was reasonable to expect that CBM1 is conducive to TtGH74 hydrolysis of the associated XG with more and longer branched chains in biomass. Thus, the yield of reducing sugars by TtGH74 alone was 45.49% higher than TtGH74∆CBM on sulfuric-acid-pretreated corn bran but only 10.88% higher on DES-pretreated corn bran, leading to the degrees of synergy between TtGH74 and cellulase mixture were 51.88% and 11.48% higher than that between TtGH74∆CBM and cellulase mixture on sulfuric-acid-pretreated and DES-pretreated corn bran, respectively (Figure 9a,b).
This further confirmed that the role and significance of CBM1 were substrate-specific because of differences in XG content and structure in various pretreated biomasses.
Corn bran (or corn fiber) is an agricultural by-product obtained from corn processing. It is well-recognized that corn bran polysaccharides are very difficult to decompose through enzymatic hydrolysis [54][55][56][57]. The recalcitrance was regarded as due to the rich content of glucuronoarabinoxylan, which was extensively decorated with variations of both monomeric and oligomeric substitutions [58].
Apple pomace is the by-product from apple processing rich in pectin, cellulose and hemicelluloses [59], and it could be a raw material for biofuel and biochemical production [60]. However, the enzymatic saccharification of apple pomace has not been well investigated.
In this study, we demonstrated that both have rich in XG, which was closely associated with other polysaccharides and remained in solid residues after sulfuric acid or DES pretreatment. The associated XG significantly hindered the enzymatic hydrolysis efficiency. Our results demonstrated that the hydrolysis performance of cellulase mixture on pretreated XG-rich biomasses could be greatly improved by adding the so-called accessory enzyme xyloglucanase to enzyme mixture, suggesting the essential of xyloglucanase in enzyme cocktail for the efficient hydrolysis of XG-rich biomasses.
Corn bran was collected from Nanyang, Henan Province, China. Destarched corn bran was prepared by amylase and papain treatment according to the method used by Rose and Inglett [62] with modifications. Amylase and papain were purchased from Imperial Jade Bio-Technology Co., Ltd. (Ningxia, China). Apple pomace was purchased from Yuanzhi Biotechnology Co., Ltd. (Shaanxi, China).

AOX GACTGGTTCCAATTGACAAG TtGH74∆CBM-R ATAGTTTAGCGGCCGCTTAGTGATGGTGATGGTGATGGTGAGATTGAGTAGCTTGAGG
The sequences encoding signal peptide, linker peptide and CBM1 in selected genes were deleted, and only the catalytic domain (CD) was selected for sequence alignment.
The cells were harvested by centrifugation at 4000 rpm for 5 min and then decanted to 50 mL buffered methanol complex (BMMY) medium (100 mM potassium phosphate, pH 6.0, 1.34% (w/v) YNB, 1% yeast extract, 2% peptone). To induce expression, methanol was added to a final concentration of 1% every 24 h. After seven days, the culture was centrifuged (10,000 rpm, 10 min) and the supernatant was then directly loaded to the Ni-NTA pre-packed gravity column (Sangon Biotech, Shanghai, China).
The purification steps were performed according to the manufacturer's manual. The purified enzyme was dialyzed at 4 • C for 12 h to exclude imidazole. The protein concentration was quantified using a Pierce BCA Protein Assay kit (Thermo Fisher, Rockford, IL, USA). Polyacrylamide (12.5%) gel electrophoresis in 0.1% SDS was performed to determine the molecular mass of the enzyme preparation. Proteins were visualized after staining with Coomassie Brilliant Blue R-250.
The deglycosylation of the recombinant protein was performed by treating the protein with Endoglycosidase H (Endo H, endo-N-acetylglucosaminidase H of Streptomyces plicatus; NEB, Ipswich, MA, USA) according to the manufacturer's instructions.
For optimizing the expression of TtGH74, the effects of temperature (24 • C, 26 • C, 28 • C, 30 • C and 32 • C), methanol concentration (0.5% to 2.0%), pH (5.0, 6.0 and 7.0) and induction time (1 to 7 days) on the TtGH74 expression were investigated step by step. For methanol concentration optimization, the pH was kept at 6.0, whereas for pH optimization, the methanol concentration was maintained at 1.5%, respectively. The culture temperature was 28 • C.

Properties of Recombinant TtGH74 and TtGH74∆CBM from P. pastoris
The enzyme activity on tamarind seed XG was measured in a 0.5 mL reaction mixture containing 2 mg XG, 10 nM TtGH74 or TtGH74∆CBM in 50 mM sodium acetate buffer (pH 5.5) at 75 • C for 15 min. The amount of reducing sugars released from the substrate was determined using the 3,5-dinitrosalicylic acid reagent (DNS) method [64]. One unit of enzyme activity was defined as the amount of enzyme that catalyzed the conversion of substrate to 1 µmol of glucose equivalent per minute.
To determine the substrate specificity of TtGH74, the following polysaccharides were selected: Tamarind seed XG, β-glucan, glucomannan, lichenan, arabinoxylan, laminarin, xylan, pectin, CMC-Na, soluble starch, chitin, Avicel and PASC. The reaction was performed at the same assay condition described above instead of the individually selected substrate. The end-products generated from tamarind seed XG by the TtGH74 hydrolysis were analyzed using matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
The optimal temperatures and pHs, thermal and pH stability of TtGH74 and TtGH74∆CBM were measured on tamarind seed XG. Optimum temperatures were determined at a range of temperature from 35 to 85 • C. Optimal pHs were determined in buffers in a range of pH 3.5-8.0 (50 mM sodium acetate buffer (pH 3.5-6.0), 50 mM sodium phosphate buffer (pH 6.0-8.0)). The maximum enzyme activity was taken as 100% and relative activities were calculated.
The thermostability of the enzymes was determined by measuring the residual enzyme activities at assay condition after preincubating the recombinant enzymes at 50 to 70 • C for a specific time. The activity of TtGH74 and TtGH74∆CBM before incubation was taken as 100% and the relative activities were calculated. The pH stability was tested by preincubating the recombinant enzyme in 50 mM buffer at various pH values (3.0-8.0) at 4 • C overnight, and the residual enzyme activities were measured as above.
To investigate the effects of metal ions on TtGH74 and TtGH74∆CBM activities, the enzyme activities were assayed at the presence of 1 mM or 5 mM metal ions (Li + , K + , Mg 2+ , Pb 2+ , Ca 2+ , Zn 2+ , Ba 2+ , Cu 2+ , Ni 2+ , Mn 2+ , Co 2+ , Fe 3+ , Al 3+ ), NH 4+ and EDTA, the reaction was conducted at 50 • C for 15 min. The K m value against tamarind seed XG was determined at 50 • C for 7.5 min, using a substrate concentration in the ranges of 0.2-6 mg/mL. The K m and other kinetic values were calculated with the GraphPad Prism 8.0 program.

Adsorption of TtGH74 and TtGH74∆CBM on Different Substrates
Differences in adsorption of TtGH74 and TtGH74∆CBM on different substrates were studied by incubating the TtGH74 or TtGH74∆CBM in the mixture volume 0.5 mL with a final concentration of 300 µg/mL protein and 10 mg substrate in 50 mM sodium acetate buffer (pH 5.5) at 0 • C for 1 h with shaking (100 rpm). The substrates were Whatman filter paper, Avicel, PASC, sulfuric-acid-pretreated corn bran, DES-pretreated corn bran and DES-pretreated apple pomace. After the incubation, the mixture was centrifuged at 10,000 rpm for 10 min.
The unbound protein in the supernatant was quantified using a Pierce BCA Protein Assay kit. The adsorption of TtGH74 on XG coated PASC was determined at the same incubation condition. The 0.5 mL mixture contained a final concentration of 300 µg/mL protein and 1 mg PASC with different proportions of XG. The XG coated PASC was prepared by premixing an aqueous solution of XG (0 to 600 (µg/mg)) with 1 mg PASC at room temperature in total 0.5 mL of 50 mM sodium acetate buffer (pH 5.5) with stirring (rotary disc contactor, 20 rpm) for 18 h [50]. The difference in binding affinity between TtGH74 and TtGH74∆CBM to XG was also determined by affinity electrophoresis (AE). AE was conducted in 7.5% polyacrylamide gels containing 0.005 w/v soluble XG at 0 • C and 150 V for 3 h [65].

Hydrolysis Action of TtGH74 and TtGH74∆CBM on XG and XG-coated PASC
The hydrolysis action of TtGH74 or TtGH74∆CBM on free XG was performed in the reaction mixture 0.5 mL containing 10 nM TtGH74 or TtGH74∆CBM and different amounts (25-600 µg) of XG in 50 mM sodium acetate buffer (pH 5.5) in the shaking incubator at 50 • C and 200 rpm for 1 h. Then the amount of reducing sugar was detected by the DNS method. The hydrolysis action of TtGH74 or TtGH74∆CBM on XG-coated PASC was performed in the same reaction condition but using XG-coated PASC as substrate.
The hydrolytic activity of EG1 or CBH1 towards XG-coated PASC was also performed in the same reaction condition but with 2.5 µg EG1 or 2.5 µg CBH1, respectively. In order to determine the presence of free XG in XG-coated PASC solution, the liquid fraction was separated by centrifugation and the unbound XG in the supernatant was extensively hydrolyzed by overdose TtGH74, and the reducing sugar was measured by the DNS method.

Analysis of the Presence of XG and Hydrolysis Action of TtGH74 or TtGH74∆CBM on Pretreated Residues
Corn bran and apple pomace were pretreated with two methods and different times. The destarched corn bran was pretreated either with 1% sulfuric acid or deep eutectic solvents (DES, lactic acid: ChCl molar ratio was 2:1). Apple pomace was also pretreated with DES as the same solution for destarched corn bran. The pretreatment temperature was 90 • C and reaction times were 3, 6, 9 and 12 h, respectively.
After pretreatment, the residues were washed with distilled water to neutral pH and dried at 105 • C to a constant weight. In order to verify whether XG still exists in the pretreated substrates, the residues with different pretreatment times were hydrolyzed by TtGH74 or TtGH74∆CBM in the 1 mL reaction mixture containing 20.7 nM of TtGH74 (15 U/g substrate) or TtGH74∆CBM and 20 mg substrate with 0.1% antibiotics (zeocin and ampicillin) in 50 mM sodium acetate buffer (pH 5.5) in the shaking incubator at 50 • C and 200 rpm for 96 h. The reducing sugars produced after hydrolysis were detected by the DNS method. The presence of XG in the above three pretreated substrates was further confirmed by analyzing the TtGH74 hydrolysis end-products using HPAEC-PAD and MALDI-TOF MS.
Finally, corn bran pretreated with sulfuric acid for 6 h, corn bran and apple pomace pretreated with DES for 9 h were used as substrates for further exploration. Time course of enzymatic hydrolysis of three pretreated substrates in different concentrations (from 10 to 80 mg) was performed by TtGH74 or TtGH74∆CBM in the same condition described above.

Statistical Analysis
All described experiments were performed in triplicate and all the reported data are means of the three samples. In addition, the Student's t-test analysis was used to determine the statistical difference between the two groups, when p < 0.05, the data difference was statistically significant.

Conclusions
In this study, we characterized and comprehensively compared TtGH74 and TtGH74∆CBM from T. terrestris. The deletion of CBM1 improved the thermostability but decreased the K cat slightly and the sample almost completely lost its adsorption capacity for insoluble carbohydrates. We also compared the hydrolysis of free XG, XG-coated PASC, pretreated corn bran and apple pomace by TtGH74 or TtGH74∆CBM action alone or in combination with other glycoside hydrolases.
Our results indicated that XG was difficult to remove and still retained with some modifications in the residues of corn bran and apple pomace after common pretreatment. CBM1 might not be essential for the hydrolysis of free XG but it is effective for the associated XG to some extent. TtGH74 could remove the bound xyloglucan on cellulose; therefore, it efficiently boosted the enzymatic hydrolysis of the pretreated XG-rich lignocellulosic biomasses.
The presence of CBM1 is conducive to enzymatic hydrolysis; however, its role and significance are substrate-specific due to the differences in the XG content and structure in various pretreated biomasses. This study deepened our understanding of the effects of CBM1 on the enzymatic properties of GH74 xyloglucanases and its necessity in the degradation of various XG-rich biomasses.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author.