Genome-Wide Identification of Cassava Glyoxalase I Genes and the Potential Function of MeGLYⅠ-13 in Iron Toxicity Tolerance

Glyoxalase I (GLYI) is a key enzyme in the pathway of the glyoxalase system that degrades the toxic substance methylglyoxal, which plays a crucial part in plant growth, development, and stress response. A total of 19 GLYI genes were identified from the cassava genome, which distributed randomly on 11 chromosomes. These genes were named MeGLYI-1–19 and were systematically characterized. Transcriptome data analysis showed that MeGLYIs gene expression is tissue-specific, and MeGLYI-13 is the dominant gene expressed in young tissues, while MeGLYI-19 is the dominant gene expressed in mature tissues and organs. qRT-PCR analysis showed that MeGLYI-13 is upregulated under 2 h excess iron stress, but downregulated under 6, 12, and 20 h iron stress. Overexpression of MeGLYI-13 enhanced the growth ability of transgenic yeast under iron stress. The root growth of transgenic Arabidopsis seedlings was less inhibited by iron toxicity than that of the wild type (WT). Potted transgenic Arabidopsis blossomed and podded under iron stress, but flowering of the WT was significantly delayed. The GLYI activity in transgenic Arabidopsis was improved under both non-iron stress and iron stress conditions compared to the WT. The SOD activity in transgenic plants was increased under iron stress, while the POD and CAT activity and MDA content were decreased compared to that in the WT. These results provide a basis for the selection of candidate genes for iron toxicity tolerance in cassava, and lay a theoretical foundation for further studies on the functions of these MeGLYI genes.


Introduction
Glyoxalase I (GLYI) is well-known for its functions as one of the main detoxifying agents of methylglyoxal (MG) produced by plants under stress [1]. Plant GLYIs are encoded by a multi-gene family, and the numbers of family members vary with species [1][2][3][4][5]. Four VtGLYI genes have been identified in the grape (Vitis vinifera) genome [2], 16 BrGLYI genes have been identified in the Chinese cabbage (Brassica rapa) genome [3], 24 GmGLYI genes have been identified in the soybean (Glycine max) genome [4], and 11 OsGLYI genes in rice (Oryza sativa) and 11 AtGLYI genes in Arabidopsis thaliana have been reported [5].
Plant GLYIs play essential roles in both biotic and abiotic stress tolerance [2,[6][7][8][9]. In grapes, the VvGLYI-1 gene is highly induced 48 h after downy mildew inoculation [2]. Overexpression of a glyoxalase-I gene (PdGLX1) from date palm (Phoenix dactylifera) in Escherichia coli can enhance its growth and MG detoxification ability. The PdGLX1 gene can also supplement the functional deletion of the MG-hypersensitive glyoxalase 1 gene -GLO1 (YML004C) yeast mutant, enhance the detoxification of MG, reduce the amassing of reactive oxygen species (ROS) under stress conditions, and promote yeast growth [9]. Several studies have reported the function of GLYIs in response and resistance to metal stress. In aluminum-treated tomatoes, a GLYI was recognized as a differentially expressed protein [10]. Overexpressing this gene can improve the tolerance of transgenic tomato plants to aluminum toxicity [7]. In Chinese cabbage, the expressions of BrGLYI genes in different heavy metal stresses have been analyzed, showing that the BrGLYI1 gene was significantly induced by ZnD, BrGLYI13 by ZnE, BrGLYI15 by FeD, BrGLYI8 by Cd, and BrGLYI3, BrGLYI6, and BrGLYI11 by Pb [3]. In wheat, TaGLYI has been shown to be induced by ZnCl 2 , and its function in tolerance to ZnCl 2 has also been verified by overexpression in tobacco [11].
Iron (Fe) is an important micro-metal element involved in various important processes of plant cells. However, excessive iron in the environment can hinder the growth and development of plants, affect the normal operation of carbon metabolism, enzyme activity, respiration, and photosynthesis, and produce ROS that cause oxidative toxicity [12,13]. The most obvious effects of iron toxicity on plant phenotypes are the inhibition of seed germination, the inhibition of the growth of roots and stems, and the reduction in yield. An amount of Fe higher than 4 mg/L reduces the germination of wheat seeds [14]. while the seed germination rate of weeds (Echinochloa crusgalli) is suppressed by 100 mg/L of Fe [15]. Apical roots are highly sensitive to iron and are the main area of iron toxicity, and excessive Fe attenuates root growth by increasing nitric oxide (NO) in the region of apical roots [16]. Nevertheless, when exposed to the same iron toxicity, tolerant rice cultivars can grow new lateral roots to rebuild their nutrient and water absorption systems, but sensitive varieties cannot [13]. Iron toxicity can reduce crop yields dramatically, such as that of rice, by up to 78% in West Africa [17]. Iron toxicity can also cause a reduction in crop spike number, spikelet sterility, and flowering delay or even failure [12,18]. In addition, under iron toxicity conditions, the activities of superoxide dismutase (SOD), peroxidase (POX), ascorbate peroxidase (APX), and glutathione reductase (GR) in the roots and shoots of iron-tolerant rice cultivars are significantly increased, while the activities of APX and catalase (CAT) in the roots of sensitive cultivars are significantly increased [19]. To regulate plant resistance to iron toxicity, some genetic data related to iron toxicity stress have been studied and obtained. Transcriptome analysis has revealed some genes related to iron homeostasis differentially expressed by iron toxicity in rice, such as OsNAS3, OsVIT2, OsFer1, and OsFer2 [12,20]. HRZ ubiquitin ligase in rice is essential for protecting cells from the iron toxicity caused by excess iron [21].
Cassava (Manihot esculenta. Crantz) is a perennial woody shrub crop with starchy storage roots in the Euphorbiaceae family, planted in tropical and subtropical areas. Cassava is important both as food and for the production of bioenergy [22]. Cassava has a certain resistance to stress, such as tolerance to drought and barren soil [23,24]. More recently, cassava has been recognized for its role in rehabilitating land contaminated with heavy metals [25]. Cassava improves soil pH and absorbs excess soluble metals such as Cd, Cu, Pb, and Zn. More importantly, the heavy metals absorbed by cassava are mainly accumulated in leaves, fibrous roots, and stems, while the accumulation is the lowest in storage roots [25]. This feature makes cassava a suitable energy crop for the remediation of metal-contaminated land.
The intention of this study was to examine the hypothesis of whether the GLYI gene has a potential function in regulating the response of cassava to iron toxicity stress. First, we systematically identified and characterized cassava's MeGLYI genes. Based on the expression patterns of said MeGLYI genes in cassava's tissues/organs, MeGLYI-13 was found to be mainly expressed in young tissues. Its expression profile in response to iron toxicity was then determined. The function of MeGLYI-13 in excessive iron toxicity tolerance was further verified by transgenic yeast and Arabidopsis. This study is expected to provide important resources for further study of the functional characteristics of MeGLYI genes and their utilization for improving the stress response of this crop.
The 19 MeGLYI genes were randomly located on 11 chromosomes of cassava ( Figure 1). There was only one MeGLYI gene on Chr1, Chr3, Chr10, Chr12, Chr14, and Chr17; two on Chr4, Chr7, and Chr11; three on Chr2; and four on Chr16. The evolution process of the MeGLYI genes was explored through collinear analysis in the genome, and a total of four collinear relationships were found ( Figure 1). The Ka/Ks value of the genes was calculated, which ranged from 0.09 to 0.79, meaning that negative selection was key to the evolution of the MeGLYI family (Table S2). to be mainly expressed in young tissues. Its expression profile in response to iron toxicity was then determined. The function of MeGLYI-13 in excessive iron toxicity tolerance was further verified by transgenic yeast and Arabidopsis. This study is expected to provide important resources for further study of the functional characteristics of MeGLYI genes and their utilization for improving the stress response of this crop.
The 19 MeGLYI genes were randomly located on 11 chromosomes of cassava ( Figure  1). There was only one MeGLYI gene on Chr1, Chr3, Chr10, Chr12, Chr14, and Chr17; two on Chr4, Chr7, and Chr11; three on Chr2; and four on Chr16. The evolution process of the MeGLYI genes was explored through collinear analysis in the genome, and a total of four collinear relationships were found ( Figure 1). The Ka/Ks value of the genes was calculated, which ranged from 0.09 to 0.79, meaning that negative selection was key to the evolution of the MeGLYI family (Table S2).  To learn the evolutionary relationships, the 19 MeGLYIs and GLYI protein sequences of 12 other plant species were aligned ( Figure 2). The 19 MeGLYIs could be grouped into four subfamilies (A-D), of which subfamily D possessed the majority of the MeGLYI members (eight), followed subfamily C containing six members, subfamily A containing three members, and the subfamily B containing two members. Gene structure analysis showed that the number of exons of the MeGLYIs ranged from two to nine, among which the maximum number of exons of MeGLYI-15, MeGLYI-16, MeGLYI-18, and MeGLYI-19 was nine ( Figure 3). The motif compositions of the 19 MeGLYI proteins were analyzed, and 10 distinct motifs were revealed ( Figure 3). In accordance with the phylogenetic analysis, the closer the evolutionary relationship between the MeGLYIs, the more similar of the motif components. Gene structure analysis showed that the number of exons of the MeGLYIs ranged from two to nine, among which the maximum number of exons of MeGLYI-15, MeGLYI-16, MeGLYI-18, and MeGLYI-19 was nine ( Figure 3). The motif compositions of the 19 MeGLYI proteins were analyzed, and 10 distinct motifs were revealed ( Figure 3). In accordance with the phylogenetic analysis, the closer the evolutionary relationship between the MeGLYIs, the more similar of the motif components.

Difference in the Expression of the MeGLYI Genes in Different Tissues/Organs of Cassava
To further detect the biological function of MeGLYIs, the expression patterns of the 19 MeGLYI genes were studied in the 11 tissues/organs of cassava, which were sourced from publicly available transcriptomic data. The MeGLYI genes were expressed in the 11 studied tissues/organs of cassava to varying degrees. In young tissues/organs such as or-

Difference in the Expression of the MeGLYI Genes in Different Tissues/Organs of Cassava
To further detect the biological function of MeGLYIs, the expression patterns of the 19 MeGLYI genes were studied in the 11 tissues/organs of cassava, which were sourced from publicly available transcriptomic data. The MeGLYI genes were expressed in the 11 studied tissues/organs of cassava to varying degrees. In young tissues/organs such as organized embryogenic structures, friable embryo callus, root apical meristems, shoot apical meristems, and lateral buds, the expression level of MeGLYI-13 was the highest. In the mature tissues/organs of storage roots, stems, petioles, midveins, and leaves, the expression level of MeGLYI-19 was the highest. Meanwhile, in fibrous roots, the expression level of MeGLYI-11 was the highest (Figure 4).

Difference in the Expression of the MeGLYI Genes in Different Tissues/Organ
To further detect the biological function of MeGLYIs, the expression p 19 MeGLYI genes were studied in the 11 tissues/organs of cassava, which from publicly available transcriptomic data. The MeGLYI genes were expre studied tissues/organs of cassava to varying degrees. In young tissues/orga ganized embryogenic structures, friable embryo callus, root apical meristem meristems, and lateral buds, the expression level of MeGLYI-13 was the h mature tissues/organs of storage roots, stems, petioles, midveins, and leav sion level of MeGLYI-19 was the highest. Meanwhile, in fibrous roots, the ex of MeGLYI-11 was the highest (Figure 4).

Expression of MeGLYI-13 in Response to Iron Stress
Since MeGLYI-13 is preferentially expressed in young tissues and organs, we were curious to analyze whether it is involved in the stress response to iron. Cassava tissue culture seedlings at the one-month stage were subjected to treatment of 450 µmol/L of FeCl 3 for 0, 2, 4, 6, 8, 12, and 24 h, separately. The mRNA levels of MeGLYI-13 expressed in the roots, shoots, and leaves were analyzed by qRT-PCR. Under high-iron treatment, the expression of MeGLYI-13 in the roots increased significantly at 2 h, but decreased significantly upon longer treatment (6, 12, and 24 h), and did not return to the normal level even after 24 h treatment ( Figure 5). In the shoots, the expression of MeGLYI-13 was significantly upregulated upon iron stress, in which the peak value was reached after 12 h of treatment. In the leaves, the expression peak value also appeared after 2 h of treatment ( Figure 5). the expression of MeGLYI-13 in the roots increased significantly at 2 h, but decreased significantly upon longer treatment (6, 12, and 24 h), and did not return to the normal level even after 24 h treatment ( Figure 5). In the shoots, the expression of MeGLYI-13 was significantly upregulated upon iron stress, in which the peak value was reached after 12 h of treatment. In the leaves, the expression peak value also appeared after 2 h of treatment ( Figure 5).

Overexpression of MeGLYI-13 in Yeast Enhanced the Tolerance to Iron Stress
To investigate the function of MeGLYI-13 in response to iron stress, the gene was expressed in INVSc1 yeast. Under normal conditions, no significant difference in growth ability was observed between the pYES2-MeGLYI-13 and pYES2 transgenic yeasts. After treatment with 20 mmol/L of FeCl3, the pYES2-MeGLYI-13 transgenic yeast grew better than the empty pYES2 vector transgenic yeast ( Figure 6). These results indicate that overexpression of MeGLYI-13 in yeast enhances the tolerance to iron stress.

Overexpression of MeGLYI-13 in Yeast Enhanced the Tolerance to Iron Stress
To investigate the function of MeGLYI-13 in response to iron stress, the gene was expressed in INVSc1 yeast. Under normal conditions, no significant difference in growth ability was observed between the pYES2-MeGLYI-13 and pYES2 transgenic yeasts. After treatment with 20 mmol/L of FeCl 3 , the pYES2-MeGLYI-13 transgenic yeast grew better than the empty pYES2 vector transgenic yeast ( Figure 6). These results indicate that overexpression of MeGLYI-13 in yeast enhances the tolerance to iron stress.

Overexpression of MeGLYI-13 in Arabidopsis Raised the Tolerance to Iron Stress
To further verify the function of MeGLYI-13 in coping with iron stress, the MeGLYI-13-overexpressing Arabidopsis was generated to study whether the expression of MeGLYI-13 could enhance the iron tolerance (Figure 7). Three out of six independent transgenic lines (OE1, OE2, and OE5) were selected for iron treatment. Both the transgenic lines and the WT lines grew well on the ½ MS medium. The inhibition of a high level of FeCl3 (400 μmol/L) on the root growth of transgenic Arabidopsis seedlings was weaker than that of the wild type (Figure 7).
To further understand the tolerance of transgenic Arabidopsis to iron stress, transgenic Arabidopsis and WT cultured in flowerpots were irrigated with 2 mmol/L of FeCl3 aqueous solution, and the growth changes were observed after 20 days of treatment (Figure 8). Under iron stress, the flowering and setting of the three transgenic Arabidopsis lines occurred earlier than those of the WT. The purple degree of old leaves in the transgenic

Overexpression of MeGLYI-13 in Arabidopsis Raised the Tolerance to Iron Stress
To further verify the function of MeGLYI-13 in coping with iron stress, the MeGLYI-13overexpressing Arabidopsis was generated to study whether the expression of MeGLYI-13 could enhance the iron tolerance (Figure 7). Three out of six independent transgenic lines (OE1, OE2, and OE5) were selected for iron treatment. Both the transgenic lines and the WT lines grew well on the 1 /2 MS medium. The inhibition of a high level of FeCl 3 (400 µmol/L) on the root growth of transgenic Arabidopsis seedlings was weaker than that of the wild type (Figure 7). plants was higher than that of the WT. The GLYI activity of the transgenic plants was significantly increased compared to that of the WT under non-or iron treatment conditions. The SOD activity of the transgenic Arabidopsis was significantly increased compared to that of the WT under iron stress conditions, while the MDA content and POD and CAT activity were lower than those of the WT, indicating that transgenic Arabidopsis suffered less stress damage. These results suggest that MeGLYI-13 could indeed play a role in resistance to excessive iron stress.  To further understand the tolerance of transgenic Arabidopsis to iron stress, transgenic Arabidopsis and WT cultured in flowerpots were irrigated with 2 mmol/L of FeCl 3 aqueous solution, and the growth changes were observed after 20 days of treatment ( Figure 8). Under iron stress, the flowering and setting of the three transgenic Arabidopsis lines occurred earlier than those of the WT. The purple degree of old leaves in the transgenic plants was higher than that of the WT. The GLYI activity of the transgenic plants was significantly increased compared to that of the WT under non-or iron treatment conditions. The SOD activity of the transgenic Arabidopsis was significantly increased compared to that of the WT under iron stress conditions, while the MDA content and POD and CAT activity were lower than those of the WT, indicating that transgenic Arabidopsis suffered less stress damage. These results suggest that MeGLYI-13 could indeed play a role in resistance to excessive iron stress.

Discussion
The GLYI family was recognized in cassava for the first time in this work, and the number (19) of gene members was more than that of grapes (4), Arabidopsis (11), rice (11), and Chinese cabbage (15) and less than that in soybean (24) and Medicago truncatula (29) [2][3][4][5]26]. Thus, species differences exist in terms of the size of the GLYI gene family.

Discussion
The GLYI family was recognized in cassava for the first time in this work, and the number (19) of gene members was more than that of grapes (4), Arabidopsis (11), rice (11), and Chinese cabbage (15) and less than that in soybean (24) and Medicago truncatula (29) [2][3][4][5]26]. Thus, species differences exist in terms of the size of the GLYI gene family. MeGLYI members can be divided into four subfamilies. The genes grouped into the same subfamily may have similar functions. In this study, the closer the MeGLYIs were to one another, the more similar their motif composition was, which further verified the reliability of the MeGLYI classification.
In previous studies, GLYI gene expression was found to be tissue-or developmental stage-specific [2][3][4]26]. In grapes, the expression level of VvGLYI-1 in leaves, VvGLYI-2 in leaves and tendrils, VvGLYI-3 in tendrils, and VvGLYI-4 in young ovules (20 days after flowering) was the highest [2]. In Chinese cabbage, the expression of BrGLYI6 and BrGLYI11 was higher in siliques, BrGLYI9 was higher in silique walls, BrGLYI5 and BrGLYII2 were higher in roots, BrGLYI8 and BrGLYI11 were higher in flower buds, and BrGLYI1, -7, -10, -14, and -15 were higher in callus [3]. In M. truncatula, the expression level of MtGLYI-4 was the highest in all 17 tissues analyzed, while the expression level of MtGLYI-3, MtGLYI-18, and MtGLYI-20 was low in all tissues. The expression of MtGLYI-4, MtGLYI-12, MtGLYI-13, MtGLYI-14, MtGLYI-15, MtGLYI-23, and MtGLYI-24 was higher in young vegetative buds [26]. Previous studies have shown that GLYI activity is related to the cell division of plant callus [3]. In our work, the expression of cassava MeGLYIs also showed different tissue specificity. More interestingly, the expression level of MeGLYI-13 was the highest in young tissues/organs such as organized embryogenic structure, friable embryo callus, root apical meristems, shoot apical meristems, and lateral buds. These results indicate that MeGLYI-13 may participate in the process of cell division.
In recent years, enhanced GLYI activity has been associated with improved plant adaption to metal stress. The synergistic application of potassium and melatonin alleviates the toxic effect of Cd stress by increasing the activity of glyoxalase I in tomato seedlings [27]. In sugarcanes, a novel glyoxalase I gene (SoGloI) has been identified, which is upregulated by CuCl 2 , NaCl, CdCl 2 , and ZnSO 4 [28]. The recombinant Escherichia coli expressing SoGloI has been shown to increase tolerance to high concentrations of CuCl 2 , CdCl 2 , NaCl, and ZnSO 4 [28]. In wheat, TaGLYI has been isolated and shown to be induced by a high concentration of ZnCl 2 [11]. After the overexpression of TaGLYI in tobacco leaves, the tolerance of transgenic tobacco to ZnCl 2 stress has been shown to be significantly enhanced [11]. Meanwhile, NaCl and ZnCl 2 stress induces OsGLYI expression in rice seedlings. The overexpression of the OsGLYI gene increases glyoxalase activity, decreases the MG level, and enhances the tolerance to NaCl and ZnCl 2 , while the seed setting rate and yield were shown to be higher than those of CK [29]. In another study, SlGLYIoverexpressing tomatoes showed significantly increased glyoxalase activity in their root tips under both Al and non-Al treatments, and the root fresh and dry weight were higher than those of the control [7]. In Chinese cabbage, the expression of BrGLYI8 and BrGLYI6 has been shown to be induced by Pb and Cd treatments [3]. However, the role of the GLYI gene in excessive iron stress has not been reported.
Previous studies have shown that apical roots are a major area of iron (Fe) toxicity and are highly sensitive to iron [16]. In this study, the MeGLYI-13 gene from cassava was found to be the most highly expressed in the root tips of cassava. Therefore, MeGLYI-13 was selected for further study in terms of the iron toxicity response. Consistent with our hypothesis, its expression was significantly induced by FeCl 3 stress after 2 h of treatment. Its function in regulating iron stress tolerance was demonstrated by expression in yeast and Arabidopsis. Further experiments will be needed to reveal the molecular mechanism of MeGLYI-13 in regulating the tolerance to iron toxicity.

Identification and Analysis of GLYI Genes in Cassava
In the cassava genome database (Manihot esculenta V6.1), GLYI family genes in cassava were searched and identified in multiple ways, by searching the keywords glyoxalase, PF00903, PF12681 and PF18029, and BLAST based on the known GLYI protein sequences in Arabidopsis thaliana. The identified genes were named in ascending order according to their position on the chromosome. The physical and chemical properties of the genes were predicted using the ProParam online website. The chromosome position and collinearity of cassava's MeGLYI genes were visualized by TBtools software v.1.089. The Ka/Ks values were calculated by KaKs_Calculator software v.2.0. The GLYI protein sequences in cassava, Arabidopsis, rice, castor bean (Ricinus communis), flax (Linum usitatissimum), grape (Vitis vinifera), Chinese cabbage, poplar (Populus trichocarpa), banana (Musa acuminata), corn (Zea mays), tomato (Solanum lycopersicum), cocoa (Theobroma cacao), and rubber tree (Hevea brasiliensis) were collected for phylogenetic analysis. MEGA 11.0 software was used to perform a multi-sequence comparison on the proteins. The neighbor-joining method was used to construct a phylogenetic tree. The test of phylogeny was used Bootstrap method with the number of bootstrap replications was 1000. The substitution model was Poisson model. The Gaps/Missing data treatment was Pairwise deletion. The GSDS website was used to illustrate a diagram of the structure of the MeGLYI genes. MEME was made to find and illustrate the conservative motifs.

Expression Analysis of MeGLYIs in Different Tissues and Organs
Transcriptome data from 11 tissues/organs (fibrous roots, storage roots, shoots, leaves, petioles, medial veins, lateral buds, root apical meristems, shoot apical meristems, organized embryogenic structure, and friable embryo callus) of cassava were used in this study (Bioproject ID PRJNA324539) [24]. The FPKM values of all of the MeGLYIs were selected and converted into log2-fold, and then heat maps were draw by TBtools software [30].

Expression Analysis of MeGLYI-13 in Response to Iron Stress
To explore the function of the MeGLYI-13 gene in iron stress, 40-day-old SC8 cassava seedlings were treated with 450 µmol/L of FeCl 3 to induce excessive iron stress. Meanwhile, 40-day-old SC8 cassava seedlings without treatment were used as the control. Root, shoot, and leaf samples were harvested at 0, 2, 6, 12, and 24 h after the iron treatment, separately. In this experiment, two plants and three biological replicates were set for each treatment. Then, all of the samples were frozen by liquid nitrogen for isolating the total RNA using a Plant Total RNA Isolation Kit Plus (Foregene). The MonScript™ RTIII Super Mix with dsDNase (Two-Step) Kit was used to remove the remaining DNA from the RNA and then for reverse transcription of the RNA into cDNA. The qRT-PCR data were reacted by SYBR ® Premix Ex TaqTM II reagent (Takara) and detected by the ABI 7900 HT Fast Real-Time PCR System. The relative expression levels of MeGLYI-13 at different times after treatment were calculated and analyzed by the popular 2 −∆∆CT method. The primers used in this experiment are listed in Table S3.

Cloning of MeGLYI-13 and Functional Analysis in Yeast and Arabidopsis
The full-length sequence of MeGLYI-13 was amplified from SC8 cassava using the primers MeGLYI-13 F: GCTAGGGCTTCTCTGCTCTG and MeGLYI-13 R: TCCCACAATCT-GACACCTGC. PCR reaction was performed using Prime STAR H5 premix (Takara). The target fragment of the cloned MeGLYI-13 gene was purified and recovered using a Gel Extraction Kit (Omega), and then sequenced.
A KpnI restriction site was designed upstream of MeGLYI-13 gene CDS, and an EcoRI restriction site was designed downstream of it. Then, the MeGLYI-13 gene was inserted into the pYES2 vector by double enzyme digestion. The pYES2-MeGLYI-13 vector was then transferred into the INVSc1 yeast strain via the PEG/LiAc transformation method. To investigate the role of MeGLYI-13 in response to excessive iron stress, the pYES2-MeGLYI-13 and control pYES2 transgenic yeasts were treated by SC/Ura liquid medium containing 20 mmol/L of FeCl 3 for 6 h. After treatment, the bacterial solution was diluted in six gradients: 10 −1 , 10 −2 , 10 −3 , 10 −4 , 10 −5 , and 10 −6 . Then, 5 µL of the diluted bacterial solution was taken from each sample, and the sample was spotted on SC-Ura medium (containing 2% galactose). After incubation at 28 • C for two days, the survival differences between the colonies were photographed and compared.
To further study the function of MeGLYI-13 in resistance to excessive iron stress, the full-length cDNA sequence of the MeGLYI-13 gene without the termination codon was connected to the plant expression vector pCAMBIA1300-35S-GFP by the homologous recombination method. The obtained vector pCAMBIA1300-35S-MeGLYI-13:GFP was transferred into Arabidopsis by the agrobacterium-mediated pollen tube immersion method. Three positive lines (OE1, OE2, and OE5) were selected for subsequent treatment. The seeds of transgenic and wild-type Arabidopsis were disinfected and germinated on 1/2 MS solid medium, vernalized at 4 • C for two days, and then kept at 22 • C under 12 h light/12 h dark conditions for one week. The seedlings were then transplanted onto 1/2 MS containing different concentrations of FeCl 3 (0, 200, 300, or 400 µmol/L) for excessive iron stress treatment, and three biological replicates were set. Twelve days later, the phenotypic changes and root length were measured. To further understand the tolerance of MeGLYI-13 transgenic Arabidopsis to FeCl 3 stress, WT and MeGLYI-13 transgenic Arabidopsis were transplanted into pots for 20 days and then watered with 2 mmol/L of FeCl 3 for another 20 days. The changes in phenotype and physiological indexes such as superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, catalase (CAT) activity, glyoxalase I activity, and peroxidase (POD) activity were then measured.