Tceal5 and Tceal7 Function in C2C12 Myogenic Differentiation via Exosomes in Fetal Bovine Serum

The proliferation and differentiation of skeletal muscle cells are usually controlled by serum components. Myogenic differentiation is induced by a reduction of serum components in vitro. It has been recently reported that serum contains not only various growth factors with specific actions on the proliferation and differentiation of myogenic cells, but also exogenous exosomes, the function of which is poorly understood in myogenesis. We have found that exosomes in fetal bovine serum are capable of exerting an inhibitive effect on the differentiation of C2C12 myogenic cells in vitro. In this process of inhibition, the downregulation of Tceal5 and Tceal7 genes was observed. Expression of these genes is specifically increased in direct proportion to myogenic differentiation. Loss- or gain- of function studies with Tceal5 and Tceal7 indicated that they have the potential to regulate myogenic differentiation via exosomes in fetal bovine serum.


Introduction
Growth arrest and terminal differentiation of skeletal muscle cells to postmitotic myotubes occur when the level of serum is decreased in the cell-culture medium. Thus, in vitro studies with myogenic cells indicate that myogenic differentiation is controlled by serum depletion [1]. Myogenic differentiation in cell culture is usually inhibited by placing myoblasts into a mitogen-rich medium, in contrast to a mitogen-poor medium that promotes differentiation. These effects are due to several growth factors such as insulin-like growth factors, fibroblast growth factors, transforming growth factor-beta, platelet-derived growth factors, and so on [2][3][4][5][6].
It has recently been reported that serum contains not only various hormones and growth factors with specific actions on the proliferation and differentiation of myogenic cells, but also exogenous exosomes that are extracellular membrane micro-vesicles derived from many different types of cells and released into body fluids including blood [7,8]. Exosomes originate from endosome and carry a complex cargo of proteins, lipid, and nucleic acids, including DNA, mRNAs, and non-coding RNAs, functioning in intercellular communication [9]. Skeletal muscle cells also release extracellular micro-vesicles into the extracellular positions which represent new paracrine or endocrine signals to communicate to other organs [10,11]. However, little is known about the function of exosomes derived from non-muscle tissues to myogenic cells. It is important to investigate whether cultures of C2C12 myogenic cells could receive exosomes as target cells. Hence, we have analyzed the effect of micro-vesicles in serum to C2C12 cell cultures. 2 of 11 In this paper, we report that exosomes in fetal bovine serum are capable of exerting an inhibitive effect on the differentiation of C2C12 myogenic cells in vitro, and Tceal5 and Tceal7 genes, encoding members of the transcription elongation factor A (Sll)-like gene family [12,13], are expressed during myogenic differentiation and are dynamically regulated by exosomes in serum. It has been reported that members of this family, with a zinc finger-like motif, are ubiquitously expressed in normal tissues (Tceal1 or Tceal4), and function as nuclear phosphoproteins to modulate the transcription in a promoter context-dependent manner. It exerts its effects via protein-protein interactions with other transcriptional regulators. Multiple family members are located on the X chromosome [12].
Our studies show that exosomes function as upstream regulators of Tceal5 and Tceal7 gene expression and indicate the role of both genes during muscle differentiation. These results define an exosome-Tceal cascade, which is important in the regulation of muscle differentiation.

Myogenic Cell Growth without Exosomes in Serum
The proliferation and differentiation of skeletal muscle cells in culture are usually controlled by serum components. To define the appropriate serum condition for the growth of C2C12 myoblast cells, we grew these cells in culture medium supplemented with different concentrations of serum. Cells cultured in 10% of FBS (fetal bovine serum) in DMEM reached confluence within a few days (Figure 1a, upper left panel), not observed with less than 10% of FBS in DMEM, as mentioned in many protocols (Figure 1a). It has been reported that many growth factors in serum can stimulate myogenic proliferation and differentiation, and recently exosomes, as well as growth factors, have been identified in serum. To evaluate the effect of exosomes in serum on myogenic cell growth, we prepared exosome-depleted serum, which is available as a commercial product, for proliferating C2C12 cells (Figure 1b, middle panels). There was no apparent morphological difference with or without exosomes in these cultures (Figure 1b, left and middle panels). C2C12 cells did not grow in non-serum conditions although the same number of cells were initially plated ( Figure 1b, right panels). Under conditions where exosomes were or were not present in the serum, the number of growing cells was similar, and the level of transcripts for markers of myogenic stem cells, myogenic commitment to differentiation, and the cell cycle showed no difference (Figure 1c,d). In this paper, we report that exosomes in fetal bovine serum are capable of exerting an inhibitive effect on the differentiation of C2C12 myogenic cells in vitro, and Tceal5 and Tceal7 genes, encoding members of the transcription elongation factor A (Sll)-like gene family [12,13], are expressed during myogenic differentiation and are dynamically regulated by exosomes in serum. It has been reported that members of this family, with a zinc finger-like motif, are ubiquitously expressed in normal tissues (Tceal1 or Tceal4), and function as nuclear phosphoproteins to modulate the transcription in a promoter contextdependent manner. It exerts its effects via protein-protein interactions with other transcriptional regulators. Multiple family members are located on the X chromosome [12].
Our studies show that exosomes function as upstream regulators of Tceal5 and Tceal7 gene expression and indicate the role of both genes during muscle differentiation. These results define an exosome-Tceal cascade, which is important in the regulation of muscle differentiation.

Myogenic Cell Growth without Exosomes in Serum
The proliferation and differentiation of skeletal muscle cells in culture are usually controlled by serum components. To define the appropriate serum condition for the growth of C2C12 myoblast cells, we grew these cells in culture medium supplemented with different concentrations of serum. Cells cultured in 10% of FBS (fetal bovine serum) in DMEM reached confluence within a few days (Figure 1a, upper left panel), not observed with less than 10% of FBS in DMEM, as mentioned in many protocols (Figure 1a). It has been reported that many growth factors in serum can stimulate myogenic proliferation and differentiation, and recently exosomes, as well as growth factors, have been identified in serum. To evaluate the effect of exosomes in serum on myogenic cell growth, we prepared exosome-depleted serum, which is available as a commercial product, for proliferating C2C12 cells (Figure 1b

Myogenic Differentiation with Exosomes from Serum
The incubation of C2C12 cells in low-serum conditions can promote myogenic differentiation and we next investigated whether this is impacted by exogenous exosomes from serum impact. Cells cultured in 10% of FBS in DMEM reached semi-confluence around 80% within a few days (Figure 2a Figure S2). These data suggest that exosomes in fetal bovine serum function to myogenic differentiation, not to cell growth. (c) C2C12 cells grow in the condition with or without exosomes (sample1-3); (d) relative mRNA expression of myogenic stem cell (Pax7), myogenic commitment to differentiation (MyoD), and cell cycle (Ccnd1, Cdkn1a, cdkn2a, Cdkn1b) markers in C2C12 cells cultured with or without exosomes in FBS. All Scale bar = 100 µm. Data represent means ± SEM (n = 3). The statistical analysis was performed by Mann-Whitney U test.

Myogenic Differentiation with Exosomes from Serum
The incubation of C2C12 cells in low-serum conditions can promote myogenic differentiation and we next investigated whether this is impacted by exogenous exosomes from serum impact. Cells cultured in 10% of FBS in DMEM reached semi-confluence around 80% within a few days (Figure 2a

Tceal5 and Tceal7 in Differentiated C2C12 Cells
To examine the effect of exosome in serum on myogenic differentiation, we performed next-generation sequencing analyses (NGS) with C2C12 cells in growing conditions with 10% of FBS, or in differentiating conditions with 0.2% of FBS or 2% of horse serum with or without added exosomes (Figure 3a,b, Supplementary Table S1). Among downregulated mRNAs expressed in differentiating C2C12 cells with the addition of exosomes, the expression levels of Tceal5 and Tceal7 were significantly lower in both NGS and RT-qPCR analyses (Figure 3c-e). These data suggest that Tceal5 and Tceal7 are upregulated in differentiating myogenic cells and that exogenous exosomes in fetal bovine serum downregulated less than half of these transcripts. Addition of exosomes derived from serum affects myogenic differentiation. (a) Approximately 80% of semi-confluent C2C12 cells with 10% or 0.2% of fetal bovine serum (FBS) with or without additional exosomes; (b) differentiated C2C12 cells (day4) were immunostained with anti-MF20 (MyHC, green). All nuclei were stained with 4 6-diamidino-2-phenylindole (DAPI, blue); (c) the ratio of DAPI-positive multinuclei staining present in single MyHC-positive myofibers with or without additional exosomes; (d) relative expression of myogenic differentiation (Myog), fusion (Mymk), and differentiated markers (Myh1, Ckm) in C2C12 cells cultured in FBS with or without additional exosomes. All Scale bar = 100 µm. Data represent means ± SEM (n = 3). The statistical analysis was performed with Mann-Whitney U test. * p < 0.05, ** p < 0.01.

Tceal5 and Tceal7 in Differentiated C2C12 Cells
To examine the effect of exosome in serum on myogenic differentiation, we performed next-generation sequencing analyses (NGS) with C2C12 cells in growing conditions with 10% of FBS, or in differentiating conditions with 0.2% of FBS or 2% of horse serum with or without added exosomes (Figure 3a,b, Supplementary Table S1). Among downregulated mRNAs expressed in differentiating C2C12 cells with the addition of exosomes, the expression levels of Tceal5 and Tceal7 were significantly lower in both NGS and RT-qPCR analyses (Figure 3c-e). These data suggest that Tceal5 and Tceal7 are upregulated in differentiating myogenic cells and that exogenous exosomes in fetal bovine serum downregulated less than half of these transcripts. serum with or without added exosomes (Figure 3a,b, Supplementary Table S1). Among downregulated mRNAs expressed in differentiating C2C12 cells with the addition of exosomes, the expression levels of Tceal5 and Tceal7 were significantly lower in both NGS and RT-qPCR analyses (Figure 3c-e). These data suggest that Tceal5 and Tceal7 are upregulated in differentiating myogenic cells and that exogenous exosomes in fetal bovine serum downregulated less than half of these transcripts.

Attenuated Tceal5 and Tceal7 Expression Effects to Myogenic Differentiation
We next examined whether the knockdown of Tceal5/7 mRNAs with small interfering RNAs (siRNAs) affected differentiating C2C12 cells in a similar manner to the addition of exosomes. The locations of target positions of siRNAs in the coding sequence (CDS) and 3′UTR of Tceal5 and Tceal7 genes are indicated in Figure 4a. Treatment with each siRNA showed the repression of the targeted transcripts specifically (Figure 4b). C2C12 cells grown with these siRNAs were morphologically normal (Figure 4c), and their level of transcripts related to myogenesis, or the cell cycle was almost identical to that of control C2C12 cells (Figure 4d). Downregulation of single Tceal5 or Tceal7 did not show significant reduction of myogenic differentiation (Supplementary Figure S3). In contrast, the treatment with both Tceal5 and Tceal7 siRNAs had an impact on differentiated myofibers in C2C12 cultures, shown as lower staining efficiency with MyHC antibodies and a reduction in transcripts of myogenic maturation markers compared to non-treated cells (Figure 4e,f). These results suggest that the attenuation of Tceal5 and Tceal7 gene expression had an effect on myogenic differentiation.

Attenuated Tceal5 and Tceal7 Expression Effects to Myogenic Differentiation
We next examined whether the knockdown of Tceal5/7 mRNAs with small interfering RNAs (siRNAs) affected differentiating C2C12 cells in a similar manner to the addition of exosomes. The locations of target positions of siRNAs in the coding sequence (CDS) and 3 UTR of Tceal5 and Tceal7 genes are indicated in Figure 4a. Treatment with each siRNA showed the repression of the targeted transcripts specifically (Figure 4b). C2C12 cells grown with these siRNAs were morphologically normal (Figure 4c), and their level of transcripts related to myogenesis, or the cell cycle was almost identical to that of control C2C12 cells (Figure 4d). Downregulation of single Tceal5 or Tceal7 did not show significant reduction of myogenic differentiation (Supplementary Figure S3). In contrast, the treatment with both Tceal5 and Tceal7 siRNAs had an impact on differentiated myofibers in C2C12 cultures, shown as lower staining efficiency with MyHC antibodies and a reduction in transcripts of myogenic maturation markers compared to non-treated cells (Figure 4e,f). These results suggest that the attenuation of Tceal5 and Tceal7 gene expression had an effect on myogenic differentiation. . The statistical analysis was performed by Mann-Whitney U test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Increased Expression of Tceal5 and Tceal7 Rescues Myogenic Differentiation from the Negative Effects of Exosomes
These studies suggest that Tceal5 and Tceal7 genes are downregulated by exosomes. To investigate further their functional role in myogenic differentiation, we examined the effects of Tceal5/7 overexpression, using vectors producing, Tceal5, HA-tagged Tceal7, and 2A peptide-based bicistronic Tceal5 and Tceal7 proteins, driven by a CAG promoter (Figure 5a). Tceal5 and Tceal7 overexpression were confirmed by RT-qPCR (Figure 5b), cleaved Tceal7-HA-tag protein was detected by Western blot analyses (Figure 5c) and Tceal7-HA was localized in the nucleus by immunostaining with anti-HA antibody (Supplementary Figure S5). To further investigate their functional roles in myogenic differentiation, C2C12 cells were seeded in growth condition with or without additional expression of Tceal5/7 factors and then exposed to myogenic differentiating condition with or without additional exosomes as shown in Figure 2a. Differentiating medium with added exosomes suppressed myogenic differentiation, however this effect was partially rescued (d) Relative RT-qPCR analyses of myogenic determination (MyoD, Myf5) and cell cycle genes (Ccnd1, Cdkn1a, Cdkn2a) in growing C2C12 cells with or without both siRNAs; (e) differentiated C2C12 cells with siRNAs were immunostained with anti-MF20 (MyHC, green). All nuclei were stained with 4 6-diamidino-2-phenylindole (DAPI, blue); (f) relative cell numbers of differentiated C2C12 cells with or without Tceal5/7 siRNAs, counted with anti-MyHC antibody and DAPI in (e); (g) relative RT-qPCR of mature myogenic transcripts (Myh1, Myh3, Ckm) in differentiating muscle cells with or without Tceal5/7 siRNAs; All Scale bar = 100 µm. Data represent means ± SEM (n = 3). The statistical analysis was performed by Mann-Whitney U test. * p < 0.05, ** p < 0.01, *** p < 0.001. Downregulation of single Tceal5 or Tceal7 did not show significant reduction of myogenic differentiation (Supplementary Figure S3). In contrast, the treatment with both Tceal5 and Tceal7 siRNAs had an impact on differentiated myofibers in C2C12 cultures, shown as lower staining efficiency with MyHC antibodies and a reduction in transcripts of myogenic maturation markers compared to non-treated cells (Figure 4e,f). These results suggest that the attenuation of Tceal5 and Tceal7 gene expression had an effect on myogenic differentiation.

Increased Expression of Tceal5 and Tceal7 Rescues Myogenic Differentiation from the Negative Effects of Exosomes
These studies suggest that Tceal5 and Tceal7 genes are downregulated by exosomes. To investigate further their functional role in myogenic differentiation, we examined the effects of Tceal5/7 overexpression, using vectors producing, Tceal5, HA-tagged Tceal7, and 2A peptide-based bicistronic Tceal5 and Tceal7 proteins, driven by a CAG promoter (Figure 5a). Tceal5 and Tceal7 overexpression were confirmed by RT-qPCR (Figure 5b), cleaved Tceal7-HA-tag protein was detected by Western blot analyses (Figure 5c) and Tceal7-HA was localized in the nucleus by immunostaining with anti-HA antibody (Supplementary Figure S5). To further investigate their functional roles in myogenic differentiation, C2C12 cells were seeded in growth condition with or without additional expression of Tceal5/7 factors and then exposed to myogenic differentiating condition with or without additional exosomes as shown in Figure 2a. Differentiating medium with added exosomes suppressed myogenic differentiation, however this effect was partially rescued by Tceal5/7-overexpression as shown by morphological elongation of myogenic cells and MyHC staining (Figure 5d-f). Transcripts of differentiated myogenic markers were upregulated in cells grown under the condition of Tceal5/7 overexpression (Figure 5g). The upregulation of single Tceal5 or Tceal7 did not rescue the phenotype that exosomes repressed myogenic differentiation (Supplementary Figure S4). These results support the hypothesis that exosomes in fetal bovine serum downregulate the expression of Tceal5 and Tceal7, which are necessary for myogenic differentiation.  (Figure 5g). The upregulation of single Tceal5 or Tceal7 did not rescue the phenotype that exosomes repressed myogenic differentiation (Supplementary Figure S4). These results support the hypothesis that exosomes in fetal bovine serum downregulate the expression of Tceal5 and Tceal7, which are necessary for myogenic differentiation.

Discussions
Proliferation and terminal differentiation of myogenic cells are usually mutually exclusive. When growing myogenic cells are induced to differentiate by being placed in a low-serum condition, they withdraw from the proliferative cell cycle because of the mitogen-poor condition. We initially tested whether exosomes, reported as novel serum components, might act as regulators of cell growth and differentiation in myogenesis. To isolate intact exosomes from FBS, we used commercial kits involving a simple and quick precipitation method, allowing them to be collected by a short, low-speed centrifugation easily applicable in most research laboratories [14]. In our approach, FBS-derived exosomes, from two different batch preparations had a significantly negative effect on myogenic differentiation, although exosome-depleted serum did not have any effect to cell growth of C2C12 cells.
The results of screening differentiated C2C12 cells, after administration of exosomes in the culture medium, showed that myogenic differentiation was compromised, with changes in gene expression. We particularly focused on Tceal5 and Tceal7 which were highly downregulated in the presence of exosomes in differentiating C2C12 cells. The Tceal, transcription elongation factor A (SII)-like family is characterized by a TFA (transcription elongation factor A) domain. This gene family includes nine members in the human genome and seven members in the mouse genome. Tceal1, the prototypical member of this family, participates in RSV (Rous sarcoma virus) long terminal repeat repression [15]. Tceal7 has been associated with tumor suppression and shown to be negatively regulated by miR-182 [16,17]. No function for Tceal5 has been reported in myogenesis, however Tceal7 was identified as a gene expressed in muscle cells and directly controlled by myogenic regulatory factors and androgen receptor [18,19]. Its overexpression negatively affected proliferation and promoted myogenic differentiation [18]. We now show that both Tceal5 and Tceal7 affect the progression of myogenic differentiation ant that their expression in differentiating muscle cells is negatively controlled by exosomes present in fetal bovine serum. The next important challenges are to identify the molecules, including microRNAs, present in exosomes derived from fetal bovine serum that regulate the expression of these factors and to determine by what mechanism these factors directly or indirectly affect myogenic differentiation, and analyze exosomes derived from murine and human sera.
Skeletal muscle tissues have a remarkable capacity to repair, however these processes, including myogenic differentiation, fail and ultimately result in the collapse of the muscles in muscular diseases such as Duchenne muscular dystrophy. The understanding of myogenic regulatory cascades is important to elucidate the molecular mechanisms of myogenic differentiation and regeneration with a view to developing innovative therapies for these patients. The presence of Tceal7 has been reported in regenerating skeletal muscle after cardiotoxin injury [18], and it will be important to follow up on this observation, as well as on the possible presence and role of Tceal5 during muscle regeneration. Our results now also add the dimension of exosome regulation of these understudied factors in the context of muscle differentiation and repair of injured muscle.
C2C12 myogenic cells were cultured in DMEM containing 10% of FBS with or without exosomes. After a few days of cell culture at 70-80% of confluency, these cells were differentiated into myotubes in DMEM supplemented with 2% horse serum (Thermo Fisher Scientific) or 0.2% FBS with or without additional exosomes derived from FBS. For example, extracted exosomal fraction derived from 5 mL of FBS were resuspended into 1ml of elution buffer, and added to 25 mL, 50 mL, 100 mL, and 250 mL of 0.2% FBS in DMEM culture medium, nearly equals the number of exosomes in 20%, 10%, 5%, and 2.5% FBS, respectively. All experiments with exosomes (0.2% FBS or 2% HS + exosome) were performed as the number of exosomes involved in 10% FBS.

Next-Generation Sequencing and RT-qPCR Analyses
Total RNAs from cultured C2C12 or 293T cells were extracted using RNeasy Micro Kit (Qiagen, Hilden, Germany). The 500 ng of total RNAs were used as starting materials to generate RNA-seq libraries with the TruSeq Stranded mRNA LT sample prep kit (Illumina, San Diego, CA, USA). The obtained libraries were sequenced on a NextSeq500 (Illumina) as 75 bp single-end reads. After trimming adaptor sequences and low-quality bases with cutadapt-1.9.1 [20], the sequenced reads were mapped to the mouse reference genome (mm10) with TopHat2 v2.1.0 [21], and expression levels were calculated as FPKM using Cufflinks v2.2.1 [22]. FPKM values are imported into GeneSpring GX software version 14.9.1 (Agilent Technologies), and differentially expressed genes were identified by with log2FC > 1 or < −1. The raw data has been deposited as GSE193702.
For quantitative PCR analyses, synthesized cDNA was prepared using the SuperScript VILO kit (Invitrogen, Waltham, MA, USA) from mRNAs. All RT-qPCR reactions were carried out in triplicate using THUNDERBIRD SYBR qPCR Mix (Toyobo, Osaka, Japan), Thermal Cycler Dice Realtime System (Takara, Shiga, Japan) and normalized to the mRNA expression level of ribosomal protein L13A (Rpl13a) as a control. Primer sequences (5 to 3 ) are listed in Supplemental Table S2.

Immunofluorescence
Cultured cells were fixed with 4% paraformaldehyde (Nacalai, Kyoto, Japan) in PBS for 10 min at 4 • C. Fixed samples were incubated with anti-MyHC antibody (MF20, R&D Systems, Minneapolis, MN, USA; diluted 1/200), anti-HA (Cell Signaling Technology; diluted 1/200) in 5% of BlockingOne (Nacalai) overnight at 4 • C. After three washes with 0.1% of Tween20 in PBS, cells were incubated with Alexa488-conjugated secondary antibodies (Molecular Probes, Eugene, OR, USA; diluted 1/500). Cells were washed and mounted in SlowFade Diamond Antifade Mountant with DAPI (Molecular Probes). Images were collected and processed to change original fluorescent colors, and count cell numbers when appropriate on the software of BZX-710 (Keyence, Osaka, Japan). For quantitation of cultured cells, the numbers of dishes were analyzed from triplicate experiments.

Tceal Overexpression and siRNA Constructs
For overexpression in C2C12 or 293T cells, pCAGGS plasmid vector (RIKEN RDB08938) was used. Tceal5 and Tceal7 coding sequences were amplified by RT-PCR with KOD FX Neo DNA polymerase (Toyobo) and single strand cDNA synthesized by SuperScript4 Reverse Transcriptase (Invitrogen) from C2C12 cells. These cDNAs were subsequently subcloned into the pTA2 vector (Toyobo) to confirm the whole sequences, and then ligated into pCAGGS vector (Tceal5 OE, Tceal7 OE). The P2A sequence was amplified by synthesized oligos with P2A-nanolantern vector [23] and cloned into the position after the deleted stop codon of Tceal5, ligated into Tceal7 CDS (Tceal5-2A-Tceal7 OE). Plasmid DNA was introduced into cells with ViaFect reagent following the manufacturer's instruction (Promega, Madison, WI, USA).

Statistics
We report the statistical data, including the results of at least three biological replicates. Statistical analyses were performed with StatPlus software (AnalystSoft, mac LE, Walnut, CA, USA) to determine significant differences with Mann-Whitney U test for two-sample comparison. p-values indicated on each figure as * p < 0.05, ** p < 0.01, and *** p < 0.001. All error bars are indicated as means ± SEM (n = 3). Funding: This research has been supported by grants-in-aid from Japan Society for the Promotion of Science (JSPS) 17K01859, Japan Agency for Medical Research and Development (AMED) JP20gm0810009, and a grant from the Nakatomi Memorial Foundation.