A Fucosylated Lactose-Presenting Tetravalent Glycocluster Acting as a Mutual Ligand of Pseudomonas aeruginosa Lectins A (PA-IL) and B (PA-IIL)—Synthesis and Interaction Studies

The Gram-negative bacterium Pseudomonas aeruginosa is an important opportunistic human pathogen associated with cystic fibrosis. P. aeruginosa produces two soluble lectins, the d-galactose-specific lectin PA-IL (LecA) and the l-fucose-specific lectin PA-IIL (LecB), among other virulence factors. These lectins play an important role in the adhesion to host cells and biofilm formation. Moreover, PA-IL is cytotoxic to respiratory cells in the primary culture. Therefore, these lectins are promising therapeutic targets. Specifically, carbohydrate-based compounds could inhibit their activity. In the present work, a 3-O-fucosyl lactose-containing tetravalent glycocluster was synthesized and utilized as a mutual ligand of galactophilic and fucophilic lectins. Pentaerythritol equipped with azido ethylene glycol-linkers was chosen as a multivalent scaffold and the glycocluster was constructed by coupling the scaffold with propargyl 3-O-fucosyl lactoside using an azide-alkyne 1,3-dipolar cycloaddition reaction. The interactions between the glycocluster and PA-IL or PA-IIL were investigated by isothermal titration microcalorimetry and saturation transfer difference NMR spectroscopy. These results may assist in the development of efficient anti-adhesion therapy for the treatment of a P. aeruginosa infection.


Introduction
Lectins are specific carbohydrate-binding proteins of non-immune origins. A common role of lectins of pathogens is an involvement in the recognition and adhesion between pathogens and hosts which are crucial processes in the development of infections [1]. Therefore, lectins from pathogens may be virulence factors and thus interesting therapeutic targets [2]. Lectins are often multivalent proteins, forming oligomers and/or containing several binding sites for interaction with other molecules. They frequently display an avidity effect resulting in a significantly increased affinity towards their native ligands, glycosylated surfaces. Thus, multivalent carbohydrate-based compounds can be suitable inhibitors of their effect [3].
Lectins are considered to be involved in the pathogenesis of the chronic pulmonary infections associated with cystic fibrosis (CF). Infection and colonization of the lungs by 2 of 14 opportunistic pathogens is essentially the main cause of mortality among individuals suffering from this hereditary disease [4]. The most widespread pathogen associated with CF is Pseudomonas aeruginosa [5]. Multidrug-resistant P. aeruginosa (PA) is included in the WHO list of pathogens as a critical priority bacterium that poses a particular threat in hospitals and causes serious and often fatal infections, such as bloodstream infections and pneumonia. Therefore, there is an urgent need to discover novel antimicrobial agents and develop therapeutic methods based on anti-adhesion targeting the lectins of PA bacteria. This bacterium produces, among other virulence factors, two soluble lectins: PA-IL (LecA) [6,7] and PA-IIL (LecB) [8]. Both proteins are tetrameric, calcium-dependent and have one carbohydrate binding site per monomer, however they differ in sequence, structure and binding specificity. PA-IL is a D-galactose-specific lectin containing one calcium ion in the binding site [9]. PA-IIL is an L-fucose-specific protein with two calcium ions in the binding site [10]. This lectin is also involved in adhesion and biofilm formation as well as its ability to block ciliary beating of epithelial cells was observed [11]. The supposed functions of PA-IL include adhesion to host cells, biofilm formation, cytotoxicity and cellular invasion [11][12][13][14].
Various types of multivalent galactose-containing inhibitors were synthesized and tested against PA-IL, including glycoconjugates with different scaffolds, modified nanoparticles and micelles [15][16][17][18][19][20][21][22][23][24]. Diverse carbohydrate-based inhibitors have also been designed against PA-IIL [21,[24][25][26][27][28][29][30]. Based on our state of knowledge, the effective inhibition of P. aeruginosa infection in vivo requires the development of novel glycomimetic for anti-adhesion therapy binding simultaneously to lectins PA-IL and PA-IIL. This could be achieved by the administration of two types of inhibitors optimized either for PA-IL or PA-IIL. A promising alternative approach is the utilization of heteroglycoclusters, i.e., compounds containing diverse carbohydrates, which could interact with both lectins. Glycosylated peptide [31] and cyclopeptide [32] dendrimers carrying mixed terminal carbohydrates and gold nanorods decorated with two types of glycomimetic polymers [33] were proposed and examined as dual inhibitors of P. aeruginosa lectins. Moreover as mutual ligands, [2] rotaxane heteroglycoclusters combining galactose and fucose subunits [34], mannosyl-centered homo-and heteroglycocluster bearing four α-L-fucoses and four β-D-galactoses and their oligonucleotide conjugates [35], homo-and hetero-bifunctional glycodendrimers ending with up to 16 fucoside and/or galactoside residues have also been prepared [36]. Another important issue of this area is related to the effect of multivalency. It is known from the literature that PA-IL is sensitive to multivalent effect [26], but in contrast, binding studies with glycofullerenes containing up to 24 fucose residue proved that lectin PA-IIL is not sensitive to cluster effect [27].
Over decades, synthesis of potential ligands of lectins from bacterial or fungal origin has been the main priority of our research. In previous studies, we demonstrated that tetravalent D-galactoside 1 [37] (Scheme 1) and its Sor Se-glycoside analogues [38] inhibited galactophilic PA-IL and we proved that compound 1 decreased P. aeruginosa adhesion to bronchial cells ex vivo. We also found that L-fucoside 2 [39,40] was a potent inhibitor of fucophilic lectin PA-IIL and has anti-adhesion properties on cellular level. The most promising anti-adhesion properties and efficacy were achieved when compound 1 was applied in combination with a tetravalent fucoside [37]. This mixture was also able to decrease adhesion of P. aeruginosa cells to bronchial human cells in the ex vivo adhesion assay.
In order to obtain an even more effective inhibitor of P. aeruginosa, we decided to synthesize a dual ligand using the same tetravalent architecture, which contains both D-galactose and L-fucose units (Scheme 1). However, unlike heteroglycoclusters, which alternately contain either fucose or galactose units on a multivalent core, we envisioned the synthesis of a combined glycocluster that contains both galactose and fucose units at each end of the multivalent scaffold. This structure can easily be synthesized if Lfucose is coupled to the natural disaccharide lactose and the trisaccharide thus obtained is attached to the multivalent scaffold (Scheme 1B). The added value of this approach is the easy and efficient synthesis of a mutual ligand of both lectins of P. aeruginosa. A tetravalent oligoglycocluster containing four D-galactoside and four L-fucoside units, such as I (Scheme 1B), may provide an excellent opportunity to test the viability of the above idea. In order to characterize the potencies of the newly synthesized glycocluster and to disclose the potential of cluster effect, if any, in the carbohydrate-protein interaction, we followed the strategy used in our previous studies, where the interaction of compounds 1 and 2 with galactophilic [37] and fucophilic lectin [39,40] were investigated by isothermal titration microcalorimetry (ITC) and saturation transfer difference NMR spectroscopy (STD-NMR) methods. In order to obtain an even more effective inhibitor of P. aeruginosa, we decided to synthesize a dual ligand using the same tetravalent architecture, which contains both Dgalactose and L-fucose units (Scheme 1). However, unlike heteroglycoclusters, which alternately contain either fucose or galactose units on a multivalent core, we envisioned the synthesis of a combined glycocluster that contains both galactose and fucose units at each end of the multivalent scaffold. This structure can easily be synthesized if L-fucose is coupled to the natural disaccharide lactose and the trisaccharide thus obtained is attached to the multivalent scaffold (Scheme 1B). The added value of this approach is the easy and efficient synthesis of a mutual ligand of both lectins of P. aeruginosa. A tetravalent oligoglycocluster containing four D-galactoside and four L-fucoside units, such as I (Scheme 1B), may provide an excellent opportunity to test the viability of the above idea. In order to characterize the potencies of the newly synthesized glycocluster and to disclose the potential of cluster effect, if any, in the carbohydrate-protein interaction, we followed the strategy used in our previous studies, where the interaction of compounds 1 and 2 with galactophilic [37] and fucophilic lectin [39,40] were investigated by isothermal titration microcalorimetry (ITC) and saturation transfer difference NMR spectroscopy (STD-NMR) methods.

Results
In the present paper, the efficient synthesis of a 3-O-fucosyl lactose-presenting tetravalent glycocluster is described and testing as an inhibitor of lectins PA-IL and PA-IIL is probed. The interactions of this molecule with both lectins were quantified by isothermal titration microcalorimetry and also characterized by saturation transfer

Results
In the present paper, the efficient synthesis of a 3-O-fucosyl lactose-presenting tetravalent glycocluster is described and testing as an inhibitor of lectins PA-IL and PA-IIL is probed. The interactions of this molecule with both lectins were quantified by isothermal titration microcalorimetry and also characterized by saturation transfer difference NMR spectroscopy.

Synthesis of Tetravalent Fucosylated Lactoside
In order to prepare an appropriate building block to the glycocluster, 3-O-α-L-fucosyl lactose (3) was synthesized according to the literature [41] and then peracetylated to obtain 4. Trisaccharide 4 was converted into propargyl glycoside using propargyl alcohol and boron trifluoride diethyl etherate as promoter to result in compound 5 with a yield of 70% (Scheme 2.). The acetyl esters were removed by Zemplén deacetylation to provide the unprotected propargyl glycoside 6, ready for azide-alkyne click reaction.

Thermodynamics of the Interactions
The results of isothermal titration calorimetry measurements providing thermodynamic characterization of the interaction of compound 8 with PA-IL and PA-IIL lectins ( Figure 1) and compound 6 with PA-IL are summarized in Tables 1 and 2, Similarly to our recent works [37][38][39][40], a pentaerythritol core 7 [40] equipped with azido ethylene glycol linkers was chosen as a multivalent scaffold. The oligoglycocluster was constructed by copper(I)-mediated 1,3-dipolar azide-alkyne cycloaddition (CuAAC) click reaction. Coupling of the tetravalent scaffold 7 with trisaccharide 6 resulted in the final glycocluster 8 with excellent yield (Scheme 3). Compounds 6 and 8 were tested as potential mutual ligands of the galactophilic PA-IL and the fucophilic PA-IIL lectins.

Thermodynamics of the Interactions
The results of isothermal titration calorimetry measurements providing thermodynamic characterization of the interaction of compound 8 with PA-IL and PA-IIL lectins ( Figure 1) and compound 6 with PA-IL are summarized in Tables 1 and 2, respectively. The corresponding data of the relevant monomeric ligands (D-Gal, Me ß-D-Gal, L-Fuc and Me ß-L-Fuc), together with those of compounds 1 and 2 are given for comparison. Compound 6 showed only slightly better affinity to PA-IL than the monomers (D-Gal or Me ß-D-Gal). On the contrary, the tetravalent glycocluster 8 was able to bind to PA-IL with two orders of magnitude higher affinity than the relevant monomers (Table 1), and its potency is commensurate with tetragalactocluster 1. These data unequivocally confirm the presence of a multivalent effect in the binding of the tetravalent

Thermodynamics of the Interactions
The results of isothermal titration calorimetry measurements providing thermodynamic characterization of the interaction of compound 8 with PA-IL and PA-IIL lectins ( Figure 1) and compound 6 with PA-IL are summarized in Tables 1 and 2, respectively. The corresponding data of the relevant monomeric ligands (D-Gal, Me ß-D-Gal, L-Fuc and Me ß-L-Fuc), together with those of compounds 1 and 2 are given for comparison. Compound 6 showed only slightly better affinity to PA-IL than the monomers (D-Gal or Me ß-D-Gal). On the contrary, the tetravalent glycocluster 8 was able to bind to PA-IL with two orders of magnitude higher affinity than the relevant monomers (Table 1), and its potency is commensurate with tetragalactocluster 1. These data unequivocally confirm the presence of a multivalent effect in the binding of the tetravalent compound 8 to PA-IL. The glycocluster 8 was able to bind to PA-IIL as well with affinity similar to tetrafucocluster 2, monovalent compound 6 and Me α-L-Fuc, but showed significantly higher affinity than L-fucose. It should be also noted that the K a value of monovalent propargyl glycoside 6 is significantly higher than those of the corresponding natural ligands in case of both lectins due to interaction of the aglycon propargyl group with the lectin binding sites. This interaction was already observed in previous studies between PA-IL and propargyl Se-ß-D-galactopyranoside [38] and PA-IIL and propargyl α-L-fucopyranoside [39], respectively. The stoichiometries (n value) of interaction in the case of tetravalent compound 8 was close to 1 in both cases (Tables 1 and 2, Figure 1), suggesting that one molecule of inhibitor is recognized by all binding sites of tetravalent PA-IL and PA-IIL. No aggregation was observed during or after calorimetry measurement implying that the binding is either intramolecular (within the same PA tetramer) or the particles resulting from intermolecular binding are too small to form visually observable aggregates in the concentration range of the measurements.
Int. J. Mol. Sci. 2022, 23, x FOR PEER REVIEW 5 of compound 8 to PA-IL. The glycocluster 8 was able to bind to PA-IIL as well with affin similar to tetrafucocluster 2, monovalent compound 6 and Me α-L-Fuc, but show significantly higher affinity than L-fucose. It should be also noted that the Ka value monovalent propargyl glycoside 6 is significantly higher than those of the correspondi natural ligands in case of both lectins due to interaction of the aglycon propargyl grou with the lectin binding sites. This interaction was already observed in previous studi between PA-IL and propargyl Se-ß-D-galactopyranoside [38] and PA-IIL and proparg α-L-fucopyranoside [39], respectively. The stoichiometries (n value) of interaction in t case of tetravalent compound 8 was close to 1 in both cases (Tables 1 and 2, Figure  suggesting that one molecule of inhibitor is recognized by all binding sites of tetravale PA-IL and PA-IIL. No aggregation was observed during or after calorimet measurement implying that the binding is either intramolecular (within the same P tetramer) or the particles resulting from intermolecular binding are too small to for visually observable aggregates in the concentration range of the measurements.

H STD Competition NMR Experiments
Saturation transfer difference NMR spectroscopy [44][45][46] is one of the most sensitive and versatile ligand-observed methods for detecting binding by NMR in the low-affinity (K D = µM-mM) range. The utility and applicability of STD method can be further extended if it is combined with competition binding experiments. Particularly, competition STD NMR experiments allows the detection of the binding over a wide affinity range, including both low-and high-affinity ligands, provides quick qualitative information on relative binding affinities and allows to characterize the binding interaction as being of specific or non-specific nature. The only requirement for the competition STD NMR experiment to work is the availability of a known low-affinity reference ligand of the protein, which has at least one STD signal well-separated from the STD resonances of other potential ligands in the mixture. In the present study, in order to characterize the binding and also the binding specificity of the monovalent fucosylated lactoside (compound 6) and the tetravalent fucosylated lactoside (compound 8) with PA-IL and PA-IIL, we performed competition STD NMR experiments using Meα-D-Gal and Me α-D-Man as STD reference ligands with known low-affinity to both lectins. Figure 2A,B show the 1D 1 H and STD NMR spectra of the reference ligand (4 mM) in the presence of PA-IL lectin (40 µM), respectively. The STD signals (highlighted by red ellipsoids) confirm the binding of Meα-D-Gal and indicate the ring protons' and also the methyl group's involvement in the interaction. Upon addition of compound 6, the STD signals of the reference ligand are significantly reduced ( Figure 2C). This result clearly demonstrates that compound 6 occupies the same or at least part of the same binding site on PA-IL as the natural reference ligand. Moreover, considering the 1:1 molar ratio of the reference and competing ligands in the sample, the substantial intensity drop (from 100% to 12%) measured on the methyl signal suggests that compound 6 shows significantly higher affinity towards PA-IL than the reference Me α-D-Gal used in the competition assay.
Interestingly, in the case of the tetravalent compound 8, we discovered a strong aggregation tendency for both PA-IL and PA-IIL lectins, approximately after 12 h, even at a relatively low concentration of the ligand (0.8 mM). The aggregation can most likely be explained by cross-linking induced via the tetravalent ligand. Therefore, in order to avoid the undesired aggregation (precipitation) of PA-IL lectin, compound 8 was introduced at significantly lower concentration into the mixture (reference ligand (4 mM), compound 8 (0.4 mM) and lectin (40 µM), respectively, i.e., in 100:10:1 molar ratio). Figure 3C illustrates the drop (or disappearance) of STD signals of the reference Meα-D-Gal ligand upon addition of compound 8. The fractional reduction (from 100% to 56%) measured on the methyl peak in 1:10 molar mixture of the competitor and reference ligand confirms the binding and higher affinity of compound 8. Interestingly, in the case of the tetravalent compound 8, we discovered a strong aggregation tendency for both PA-IL and PA-IIL lectins, approximately after 12 h, even at a relatively low concentration of the ligand (0.8 mM). The aggregation can most likely be explained by cross-linking induced via the tetravalent ligand. Therefore, in order to avoid the undesired aggregation (precipitation) of PA-IL lectin, compound 8 was introduced at significantly lower concentration into the mixture (reference ligand (4 mM), compound 8 (0.4 mM) and lectin (40 μM), respectively, i.e., in 100:10:1 molar ratio). Figure 3C illustrates the drop (or disappearance) of STD signals of the reference Meα-D-Gal ligand upon addition of compound 8. The fractional reduction (from 100% to 56%) measured on the methyl peak in 1:10 molar mixture of the competitor and reference ligand confirms the binding and higher affinity of compound 8.  Similar competition STD NMR experiments were performed with compounds 6 and 8 in the presence of the other lectin, PA-IIL using Meα-D-Man as the reference ligand. It is known from the literature [47] that this molecule also binds to the fucosyl recognition domain of the lectin but with lower affinity than Me α-L-Fuc. As before, the reduction in STD signal of the reference ligand confirmed the competitive binding of both the monoand tetravalent ligands (representative spectra are shown in Figure S1 and S2 in the Supporting Information). In addition, specific evidence of lectin precipitation due to cross- Similar competition STD NMR experiments were performed with compounds 6 and 8 in the presence of the other lectin, PA-IIL using Meα-D-Man as the reference ligand. It is known from the literature [47] that this molecule also binds to the fucosyl recognition domain of the lectin but with lower affinity than Me α-L-Fuc. As before, the reduction in STD signal of the reference ligand confirmed the competitive binding of both the mono-and tetravalent ligands (representative spectra are shown in Figures S1 and S2 in the Supporting Information). In addition, specific evidence of lectin precipitation due to cross-linking induced by the tetravalent ligand (compound 8) is given in Table S1 of SI, where the relative STD signal intensities measured on the methyl signal of the reference ligand are given as a function of the concentration of compound 8 (Figure 4). Moreover, STD experiments under identical conditions were repeated on the last sample, containing compound 8 in 4 mM concentration and STD signal intensities were monitored as a function of time. As expected, the gradual reduction of the methyl STD intensity of the reference ligand upon adding compound 8 up to 0.4 mM confirmed the competitive binding of the tetravalent ligand. However, adding more ligand to the mixture, the reference methyl STD signal started to increase, which can be most likely due to the aggregation and subsequent precipitation of the lectin, leading to higher reference ligand: lectin molar ratio, whilst the corresponding ratio for the tetravalent ligand (compound 8) is shifted in the opposite direction due to its involvement in cross-linking. Compound 5 (700 mg, 0.77 mmol) was dissolved in MeOH (10 mL), catalytic amo of NaOMe (pH~9) was added and stirred overnight at room temperature. The react mixture was neutralized with Amberlite IR-120 H + ion-exchange resin, filtered a evaporated, then the crude product was purified by flash column chromatography (83 CH3CN:H2O) to give compound 6 (279 mg, 69%) as a colourless syrup.

Discussion
As a result, click-chemistry approach was applied to synthesize tetravalent glycodendrimers bearing four 3-O-α-L-fucosylated lactose units, as potential ligand of lectins PA-IL (LecA) and PA-IIL (LecB) isolated from P. aeruginosa, with fucophilic and galactophilic character, respectively. To the best of our knowledge, no example can be found in the literature where oligosaccharides are coupled to oligovalent structure decorated with terminal D-galactose and L-fucose units. The ability of the synthetized tetravalent glycoclusters to interact with lectins PA-IL (LecA) and PA-IIL (LecB) from Pseudomonas aeruginosa was proved, and the tetravalent compound 8 was found to be suitable ligand of the lectins in vitro, with significantly better binding affinity than simple D-galactose or L-fucose. Although affinity of the novel compound is commensurate with previously synthesized tetravalent galacto-and fucocluster (1 and 2), we expect that a combined synergic effect might evolve ex vivo or cellular level. The ITC measurements established that the tetravalent glycocluster 8 bound to lectin PA-IL with K a value of two orders of magnitude higher than their natural ligands, which confirms the presence of cluster effect for compound 8. On the contrary, the compound 8 binding affinity toward lectin PA-IIL was similar to that of Me α-L-Fuc, propargyl 3-O-fucosyl lactose 6 and only slightly more than 20-times better than that of L-fucose. These results clearly confirm the hypothesis that PA-IL is sensitive to a multivalent effect, while PA-IIL seems to be independent from the cluster effect. Moreover, the stochiometric data shown (n values are around 1 in both cases) implicate, that there are interactions between the ligand and the protein in a one-to-one complex, meaning all binding sites of lectin are saturated by the ligand at the low-concentration range. On the other hand, bindings involving more lectins leading to cross-linking and self-aggregation could be expected under therapeutic conditions, at higher concentration range. In such case the mutual tetravalent glycocluster 8 exposing either the galactose or the fucose unit may allow a simultaneous binding to any of the two lectins on the bacterial surface. Using competition STD-NMR experiments, we have unambiguously confirmed the specific binding of the mono-and tetravalent ligands to both lectins. The reduction (or disappearance) in the STD signals of the low-affinity reference ligand, Meα-D-Gal or Meα-D-Man upon titration proved that both ligands compete with the reference ligand for the same (or at least overlapping) binding site of PA-IL and PA-IIL lectins. In addition, the relative intensity reduction in STD signals of the reference ligand allowed a qualitative rank ordering of the ligands and confirmed, in agreement with the ITC results, the higher affinity of the tetravalent ligand. The unusual concentration and time dependence of STD effects observed on the reference ligand signals upon titrating with compound 8 in the presence of either PA-IL or PA-IIL suggests the occurrence of cross-linking of lectins through the tetravalent ligand leading to the formation of larger aggregates and subsequent partial precipitation of lectins. The anti-adhesion therapy requires extremely high concentrations (up to 0.1 M) of ligand [48], when self-aggregation and cross-linking of lectins caused by a multivalent ligand is probably the most preferable interaction. Application of a mutual multivalent ligand may promote to evolve such intermolecular interactions via the terminal galactose and fucose residues furnished by different arms of the tetravalent architecture, making potentially feasible the simultaneous binding of both lectins on the bacterial surface to prevent colonization. However, further studies at cellular levels are still needed to assess the potential applications of these glycomimetics with great anti-adhesive properties in animal studies [49] or human therapy of PA infections [48].

General Methods
Optical rotations were measured at room temperature with a Perkin-Elmer 241 automatic polarimeter (PerkinElmer, Waltham, MA, USA). TLC analysis was performed on Kieselgel 60 F 254 (Merck, Kenilworth, NJ, USA) silica gel plates with visualization by immersing in a sulfuric acid solution (5% in EtOH, VWR International Ltd., Radnor, PA, USA) followed by heating. Column chromatography was performed on silica gel 60 (Merck 0.063-0.200 mm), flash column chromatography was performed on silica gel 60 (Merck 0.040-0.063 mm). Gel filtration was performed on Sephadex G-25, using methanol as the eluent. Organic solutions were dried over MgSO 4 and concentrated under vacuum. The 1 H (400 and 500 MHz) and 13  Lectins in recombinant form were produced and purified as previously described [9,10] by Wimmerová group (Brno, Masaryk University).

Isothermal Microcalorimetry
All experiments were performed on MicroCal iTC200 (Malvern Instruments, Malvern, UK) at 25 • C. The freeze-dried lectins were dissolved in 0.1 M Tris/HCl, 500 µM CaCl 2 , pH 7.5, and equilibrated at room temperature for 1 h before ITC measurements. Monoand multivalent compounds 6 and 8 were diluted in the same buffer and used in 0.1 mM concentrations. Aliquots of 2 µL of the compounds were added automatically to the 0.01 mM PA-IL or 0.011 mM PA-IIL in the calorimeter cell. Blank experiments (injections of compounds to the buffer) were performed and heat responses were subtracted. Integrated heat effects were analyzed by global fitting of the data obtained from three independent titrations for each tested compound by non-linear regression using a single site-binding model (Microcal Origin 7, Malvern Instruments, Malvern, UK). The fitted data yielded the association constant (K a ), the enthalpy of binding (∆H) and entropy of binding (∆S). Other thermodynamic parameters, i.e., changes in free energy (∆G), were calculated from the equation: ∆G = ∆H−T∆S = −RTln K a , where T is the absolute temperature and R = 8.314 J mol −1 K −1 .

1 H STD Competition NMR Experiments
All STD NMR experiments were performed on a Bruker Avance Neo 700 MHz spectrometer (Bruker, Billerica, MA, USA) equipped with 5 mm z-gradient TCI inverse detection Prodigy probe. The pseudo 2D pulse program stddiff·3 available in the pulse sequence library of TopSpin 4.0.5 was used for the measuments. Samples were prepared in D 2 O/20 mM deuterated Tris-buffer (150 mM NaCl, 5 mM CaCl 2 , pH = 7,5, T = 283 K). The protein resonances were selectively irradiated using Gauss (90 • ) pulses with a length of 50 ms. The total duration of saturation was set to 3 s. The on-and off-resonance saturation frequency of the selective pulse was switched between −0.5 and −40 ppm after each scan and the corresponding FIDs were collected in separate memories for subsequent processing and generation of STD spectra. A spin-lock pulse with 10 kHz field and 50 ms duration was used to suppress the residual protein signals. Reference experiments were carried out to assure the absence of direct irradiation of the ligand. STD spectra were acquired with 3200 transients and 16k data points and zero filled up to 64k data prior processing. To increase the S/N ratio the FIDs were multiplied with an exponential function (lb = 6 Hz). The samples used for STD measurements contained 40 µM lectin (PA-IL or PA-IIL), 4 mM Meα-D-Gal or Meα-D-Man as references, and 4mM compound 6 (1:100:10 molar ratio) or 0.4 mM compound 8 (1:10:100), respectively.

Conclusions
A highly efficient synthesis of a mutual, 3-O-fucosylated lactose-containing tetravalent ligand of both lectins isolated from Pseudomonas aeruginosa was developed. The potency of the mutual ligand was compared to the potency of mixed ligands, when tetravalent fucoside and galactoside were used in combination. Although the novel mutual tetravalent glycocluster did not show the expected higher potency in vitro, its pertinent binding properties to lectin PA-IL with favorable clustering and cross-linking effects make it a promising candidate for further testing at the cellular level with a potential utilization as a prophylactic agent against bacterial colonization of the lungs.