Synthesis of Glycolysis Inhibitor PFK15 and Its Synergistic Action with an Approved Multikinase Antiangiogenic Drug on Human Endothelial Cell Migration and Proliferation

Activated endothelial, immune, and cancer cells prefer glycolysis to obtain energy for their proliferation and migration. Therefore, the blocking of glycolysis can be a promising strategy against cancer and autoimmune disease progression. Inactivation of the glycolytic enzyme PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase) suppresses glycolysis level and contributes to decreased proliferation and migration of cancer (tumorigenesis) and endothelial (angiogenesis) cells. Recently, several glycolysis inhibitors have been developed, among them (E)-1-(pyridin-4-yl)-3-(quinolin-2-yl)prop-2-en-1-one (PFK15) that is considered as one of the most promising. It is known that PFK15 decreases glucose uptake into the endothelial cells and efficiently blocks pathological angiogenesis. However, no study has described sufficiently PFK15 synthesis enabling its general availability. In this paper we provide all necessary details for PFK15 preparation and its advanced characterization. On the other hand, there are known tyrosine kinase inhibitors (e.g., sunitinib), that affect additional molecular targets and efficiently block angiogenesis. From a biological point of view, we have studied and proved the synergistic inhibitory effect by simultaneous administration of glycolysis inhibitor PFK15 and multikinase inhibitor sunitinib on the proliferation and migration of HUVEC. Our results suggest that suppressing the glycolytic activity of endothelial cells in combination with growth factor receptor blocking can be a promising antiangiogenic treatment.


Introduction
The alteration of metabolism and increased level of glucose metabolism in activated endothelial cells points to glycolysis as a possible target to inhibit pathological angiogenesis. Endothelial and tumor cells utilize glycolysis to metabolize glucose to lactate instead of oxidative phosphorylation, a phenomenon known as the Warburg effect, which provides an anabolic substrate to cover the demand for rapid cell proliferation and migration.

Effect of PFK15 and sunitinib on HUVEC Migration (Wound Healing Assay)
Cell migration was quantified after the cell treatment with PFK15 and sunitinib alone or in combination. The combined action of PFK15 (at 5 or 10 µM) with sunitinib (at 0.1 or 1 µM) more efficiently inhibited HUVEC migration compared to PFK15 or sunitinib alone. Simultaneous administration of PFK15 and sunitinib at specific concentrations clearly showed a synergistic effect on the inhibition of HUVEC migration (Figure 2; representative photos illustrating cell migration are available in Supplementary Material, Figure S8).

Effect of PFK15 and sunitinib on HUVEC Proliferation
In the next experiments, we studied changes in HUVEC proliferation in the presence of PFK15 and sunitinib in order to confirm their inhibitory and synergic effect. In the preliminary experiment, we applied PFK15 at concentrations of 5 and 10 µM and sunitinib at 0.1 and 1 µM (similarly to the previous wound healing assay), alone or in combination, but in this case, the effects of inhibitors applied alone were too strong to see any combined effects (see the pictures in Supplementary Materials, Figure S9).
Therefore, in the next experiments we selected lower concentrations of PFK15 (2, 3, and 4 µM) and sunitinib (4, 5, and 6 µM) alone or in combinations. In these trials, both PFK15 and sunitinib alone reduced cell proliferation compared to the control. Moreover, simultaneous administration of both inhibitors at specific concentrations showed a synergistic effect on cell proliferation compared to application of the inhibitors alone. PFK15 at

Effect of PFK15 and Sunitinib on HUVEC Migration (Wound Healing Assay)
Cell migration was quantified after the cell treatment with PFK15 and sunitinib alone or in combination. The combined action of PFK15 (at 5 or 10 µM) with sunitinib (at 0.1 or 1 µM) more efficiently inhibited HUVEC migration compared to PFK15 or sunitinib alone. Simultaneous administration of PFK15 and sunitinib at specific concentrations clearly showed a synergistic effect on the inhibition of HUVEC migration ( Figure 2; representative photos illustrating cell migration are available in Supplementary Material, Figure S8).

Effect of PFK15 and sunitinib on HUVEC Migration (Wound Healing Assay)
Cell migration was quantified after the cell treatment with PFK15 and sunitinib alone or in combination. The combined action of PFK15 (at 5 or 10 µM) with sunitinib (at 0.1 or 1 µM) more efficiently inhibited HUVEC migration compared to PFK15 or sunitinib alone. Simultaneous administration of PFK15 and sunitinib at specific concentrations clearly showed a synergistic effect on the inhibition of HUVEC migration ( Figure 2; representative photos illustrating cell migration are available in Supplementary Material, Figure S8).

Effect of PFK15 and sunitinib on HUVEC Proliferation
In the next experiments, we studied changes in HUVEC proliferation in the presence of PFK15 and sunitinib in order to confirm their inhibitory and synergic effect. In the preliminary experiment, we applied PFK15 at concentrations of 5 and 10 µM and sunitinib at 0.1 and 1 µM (similarly to the previous wound healing assay), alone or in combination, but in this case, the effects of inhibitors applied alone were too strong to see any combined effects (see the pictures in Supplementary Materials, Figure S9). Therefore, in the next experiments we selected lower concentrations of PFK15 (2, 3, and 4 µM) and sunitinib (4, 5, and 6 µM) alone or in combinations. In these trials, both PFK15 and sunitinib alone reduced cell proliferation compared to the control. Moreover, simultaneous administration of both inhibitors at specific concentrations showed a synergistic effect on cell proliferation compared to application of the inhibitors alone. PFK15 at Statistical differences among groups were determined by one-way ANOVA followed by Tukey's post hoc test. Data are presented as the mean ± SEM of three independent experiments performed in quadruplicate (** p < 0.01; *** p < 0.001).

Effect of PFK15 and Sunitinib on HUVEC Proliferation
In the next experiments, we studied changes in HUVEC proliferation in the presence of PFK15 and sunitinib in order to confirm their inhibitory and synergic effect. In the preliminary experiment, we applied PFK15 at concentrations of 5 and 10 µM and sunitinib at 0.1 and 1 µM (similarly to the previous wound healing assay), alone or in combination, but in this case, the effects of inhibitors applied alone were too strong to see any combined effects (see the pictures in Supplementary Materials, Figure S9). Therefore, in the next experiments we selected lower concentrations of PFK15 (2, 3, and 4 µM) and sunitinib (4, 5, and 6 µM) alone or in combinations. In these trials, both PFK15 and sunitinib alone reduced cell proliferation compared to the control. Moreover, simultaneous administration of both inhibitors at specific concentrations showed a synergistic effect on cell proliferation compared to application of the inhibitors alone. PFK15 at 3 and 4 µM in combination with sunitinib at 4, 5, and 6 µM most efficiently inhibited HUVEC proliferation ( Figure 3B,C).

Discussion
The limited efficacy of the 1 st generation of antiangiogenic therapy led to the development of selective PFKFB3 inhibitors that showed potential activity against the key glycolysis enzyme in different cell types, as well as in various animal models [2,5,6]. Recently, several glycolytic inhibitors were identified; among them, PFK15 showed important antitumor efficiency. However, until now, no study has described the synthesis of this generally available glycolytic inhibitor in detail. Therefore, we performed an efficient synthesis of PFK15 and confirmed its efficiency on activated HUVEC cells. Additionally, we observed more effective inhibition of HUVEC proliferation and migration using a combination of glycolysis inhibitor PFK15 with multikinase inhibitor sunitinib, targeting different biological pathways and explaining the synergic effect of their inhibition.
3PO is one of the first synthesized blockers of glycolysis [1], and its effective synthesis has already been described by our research group [13]. Although its antiangiogenic potential was described in several studies, the concentration at which 3PO is effective appears to be relatively high. Moreover, this high concentration is difficult to achieve in clinical studies because of the poor solubility of 3PO in water [14]. Therefore, other inhibitors of PFKFB3 have been developed. Substitution of the pyridine-4-yl ring in the inhibitor 3PO for the quinoline-2-yl group results in a more powerful inhibitor PFK15, which is also characterized by higher selectivity for the enzyme PFKFB3 [2]. The antitumor effect of PFK15 was compared with the effect of chemotherapy drugs that are approved by the Food and Drug Administration (FDA) for the treatment of human cancer (e.g., irinotecan, temozolomide, and gemcitabine). The PFK15 inhibitor suppressed pancreatic and colon adenocarcinoma growth, similarly to gemcitabine and irinotecan, while the effect of PFK15 on glioblastoma growth was lower compared to the chemotherapeutic agent temozolomide [2]. The activity of the PFKFB3 enzyme can be regulated by several factors, including the AMPK signaling pathway, which can function as an upstream of the PFKFB3 regulator. It was confirmed that PFK15 is able to inhibit AMPK and Akt-mTORC1 signaling in colorectal cancer cells [15], and thereby reduce tumor cell viability.
Limitations related to low inhibitor water solubility may be solved using nanocarriers or by chemical modification of the inhibitor structure [14]. Moreover, the low cytotoxic effect of glycolysis inhibitors on healthy cells [6], confirmed in several studies [16,17], suggests that the combination of a glycolysis inhibitor with other chemotherapy agents (e.g., multikinase inhibitor or cytostatic drug) may be an important strategy in advanced cancer treatment.

Discussion
The limited efficacy of the 1st generation of antiangiogenic therapy led to the development of selective PFKFB3 inhibitors that showed potential activity against the key glycolysis enzyme in different cell types, as well as in various animal models [2,5,6]. Recently, several glycolytic inhibitors were identified; among them, PFK15 showed important antitumor efficiency. However, until now, no study has described the synthesis of this generally available glycolytic inhibitor in detail. Therefore, we performed an efficient synthesis of PFK15 and confirmed its efficiency on activated HUVEC cells. Additionally, we observed more effective inhibition of HUVEC proliferation and migration using a combination of glycolysis inhibitor PFK15 with multikinase inhibitor sunitinib, targeting different biological pathways and explaining the synergic effect of their inhibition.
3PO is one of the first synthesized blockers of glycolysis [1], and its effective synthesis has already been described by our research group [13]. Although its antiangiogenic potential was described in several studies, the concentration at which 3PO is effective appears to be relatively high. Moreover, this high concentration is difficult to achieve in clinical studies because of the poor solubility of 3PO in water [14]. Therefore, other inhibitors of PFKFB3 have been developed. Substitution of the pyridine-4-yl ring in the inhibitor 3PO for the quinoline-2-yl group results in a more powerful inhibitor PFK15, which is also characterized by higher selectivity for the enzyme PFKFB3 [2]. The antitumor effect of PFK15 was compared with the effect of chemotherapy drugs that are approved by the Food and Drug Administration (FDA) for the treatment of human cancer (e.g., irinotecan, temozolomide, and gemcitabine). The PFK15 inhibitor suppressed pancreatic and colon adenocarcinoma growth, similarly to gemcitabine and irinotecan, while the effect of PFK15 on glioblastoma growth was lower compared to the chemotherapeutic agent temozolomide [2]. The activity of the PFKFB3 enzyme can be regulated by several factors, including the AMPK signaling pathway, which can function as an upstream of the PFKFB3 regulator. It was confirmed that PFK15 is able to inhibit AMPK and Akt-mTORC1 signaling in colorectal cancer cells [15], and thereby reduce tumor cell viability.
Limitations related to low inhibitor water solubility may be solved using nanocarriers or by chemical modification of the inhibitor structure [14]. Moreover, the low cytotoxic effect of glycolysis inhibitors on healthy cells [6], confirmed in several studies [16,17], suggests that the combination of a glycolysis inhibitor with other chemotherapy agents (e.g., multikinase inhibitor or cytostatic drug) may be an important strategy in advanced cancer treatment.
The additive inhibitory effect of the simultaneous administration of 3PO and sunitinib on angiogenesis was demonstrated under in vivo conditions on a zebrafish embryo model, in which the formation of intersomitic vessels was evaluated [16]. Our previously published data [4] also confirmed that cells treated with the glycolysis inhibitor 3PO at 10 µM and sunitinib at 1 µM, as well as 20 µM 3PO in combination with sunitinib in a concentration range of 0.1-10 µM, significantly reduced cell proliferation compared to the inhibitors applied alone.
We determined the IC 50 value for both PFK15 and sunitinib ( Figure 1) and have observed a significant decrease of HUVECs migration and proliferation after cell treatment with PFK15 and sunitinib (Figures 2 and 3). To confirm the synergic effect of the combined administration of both inhibitors, PFK15 and sunitinib were applied at different concentrations. PFK15 applied at 3 and 4 µM and sunitinib at 4, 5, and 6 µM more efficiently lowered cell proliferation compared to the inhibitors alone ( Figure 3).
In addition to the simultaneous inhibition of the dominant metabolic pathway (e.g., by PFK15) and the activity on human kinase receptors (e.g., by sunitinib), there are also other possibilities to reduce cell metabolism and other physiological processes in cells. The inhibition of glycolysis in tumor cells may lead to the cells showing the increased preference for another metabolic pathway for ATP production, e.g., oxidative phosphorylation. The simultaneous inhibition of glycolysis and oxidative phosphorylation using metformin and 2-DG (2-deoxyglucose) showed an additive effect compared to the application of these inhibitors alone [18]. Another possibility is the use of phenformin, which was clinically tested in the treatment of diabetes, and oxamate, which acts as an inhibitor of lactate dehydrogenase enzyme activity. A synergistic effect was observed in the combined effect of phenformin and oxamate, where a significant antitumor effect was confirmed by simultaneous inhibition of complex I in mitochondria and LDH activity in the cytosol of the cells [17].
We used the IC 50 value, which expresses the concentration of tested substance causing inhibition of cell growth by 50%, to determine the toxicity of sunitinib and the glycolysis blocker PFK15. The measured values show that HUVEC are more sensitive to sunitinib compared to PFK15. Similar results were obtained with bovine aortic endothelial cells (BAEC) at the IC 50 concentration of 2.2 µM of sunitinib and 1.6 µM for HUVEC, respectively (Table 1). This reflects the multi-targeted tyrosine kinase inhibitory action of sunitinib compared with PFK15. The multikinase inhibitor sunitinib is used in the clinical treatment of several types of cancer and its effect has been documented by many published data [19,20]. The mechanism of action of sunitinib includes several signaling pathways, possessing receptor as well as non-receptor tyrosine kinases (e.g., VEGF-R, PDGF-R, CSFR, c-KIT, etc.) [21].
Consistent with our results, the different effectivity of 3PO and PFK15 was shown in a model of an immortalized human T-lymphocyte line (Jurkat cells) and non-small cell lung carcinoma cells (H522) after 48 h of treatment, with PFK15 having a more pronounced effect than 3PO [2]. Previously published data suggest that the effect of these inhibitors varies significantly depending on the cell type and origin (human vs. animal cells), degree of differentiation, inhibitor concentrations, and time of incubation [1,2,12,22]. Our previous study showed that PFK15 reduced HUVEC and colorectal adenocarcinoma DLD1 cell proliferation at the same level, indicating that the same molecular targets are affected by this treatment [8]. However, compared to an animal model of BAEC, the effectivity of PFK15 was quite different, as the IC 50 value for BAEC was determined to be 7.4 µM. 3PO showed similar results to PFK15, with an IC 50 value of 6.9 µM, indicating that the effectivity of glycolysis inhibition depends on several additional factors.
The simultaneous administration of PFK15 and the chemotherapeutic drug paclitaxel (PTX) showed a synergistic effect and may be an effective strategy in cancer treatment. Both drugs were carried to the cells by solid lipid nanoparticles encapsulated in membranes formulated by fusing breast cancer cell and activated fibroblast membranes [23]. The cytotoxic effect of PTX/PFK15 in solid lipid nanoparticles was confirmed in vitro, as well as in vivo. In tumor-bearing mice, the monotherapy of nanoparticles with PTX or PFK15 did not induce a significant therapeutic effect; however, mice treated with PTX/PFK15 nanoparticles showed enhanced tumor inhibition. These data suggest that the dual targeting of cancer cell and cancer-associated fibroblasts (CAF) is more effective, as the energy supply from CAF to cancer cells is blocked by glycolysis inhibition [23].
In summary, we developed the missing synthesis of PFK15 with the aim of making this glycolysis inhibitor broadly available in large amounts for in vitro and in vivo biological studies. Additionally, PFK15 was also thoroughly physicochemically characterized. From a biological point of view, we proved sufficient sensitivity of HUVEC proliferation and migration to both inhibitors alone. More importantly, we have proven the synergistic effect on HUVEC proliferation and migration with the combination of PFK15 and sunitinib at their appropriate concentrations. In future research, the pharmacokinetic properties of PFK15 should be improved by developing more biologically active form of inhibitor, which can be tested either alone and/or in combination with other clinically used drugs in different pathophysiological conditions.

General Conditions
The starting chemicals for syntheses were purchased from Sigma-Aldrich (St. Louis, MO, USA). Other chemicals and solvents were purchased from local commercial sources and were of analytical grade quality. The melting point was measured by a digital melting point apparatus Barnstead Electrothermal IA9200 and is uncorrected. 1 H-and 13 C-NMR spectra were recorded on a Varian Gemini (600 and 150 MHz, respectively); chemical shifts are given in parts per million (ppm); tetramethylsilane was used as an internal standard; and DMSO-d 6 was used as the solvent. Infrared (IR) spectra were acquired by Fourier transform infrared spectroscopy (FT-IR) attenuated total reflectance REACT IR 1000 (ASI Applied Systems) with a diamond probe and MTS detector. Mass spectra were performed on a liquid chromatography mass spectrometer LC-MS using an Agilent Technologies 1200 Series equipped with mass spectrometer Agilent Technologies 6100 Quadrupole LC-MS. The course of the reactions was followed by thin-layer chromatography (TLC) (Merck Silica gel 60 F254), and a UV lamp (254 nm), and iodine vapors were used for visualization of the TLC spots.

Synthesis of 2-Bromo-1-(pyridin-4-yl)ethan-1-one hydrobromide (2)
A solution of 32.0 g (264 mmol, 1.00 mol eq) 1-(pyridin-4-yl)ethan-1-one (1) in 250 mL glacial acetic acid (100%) was cooled in an ice bath, and 150 mL conc. HBr (48 w/w %) was carefully added. Subsequently, a solution of 42.2 g (264 mmol, 1.00 mol eq) Br2 in 20 mL glacial acetic acid was added dropwise to the reaction mixture. The reaction was stirred overnight at rt while a white precipitate formed. Afterwards, 250 mL Et2O was added, and the mixture was stirred for 30 min at rt and filtered. The obtained solid material was washed with 150 mL Et2O, dried to yield 70.0 g (249 mmol, 94%) of 2 in the form of a white solid powder. Salt 2 was used in the next step without further purification. Its m.p., 1 H-NMR [24]and MS [25] spectra are described in the literature.

Synthesis of (2-Oxo-2-(pyridin-4-yl)ethyl)triphenylphosphonium bromide (3)
First of all, 65.4 g (249 mmol, 1.00 mol eq) PPh3 and 34.7 mL (25.2 g, 249 mmol, 1.00 mol eq) Et3N were sequentially added to a solution of 70.0 g (249 mmol, 1.00 mol eq) 2 in 400 mL of THF. The reaction mixture was refluxed overnight while an orange precipitate formed. After cooling the mixture to rt, the solid material was filtered off, washed with 150 mL Et2O, and dried under reduced pressure to yield 95.9 g (207 mmol, 83%) of an orange product 3, which was used without further purification in the next reaction.

Synthesis of 1-(Pyridin-4-yl)-2-(triphenyl-λ 5 -phosphanylidene)ethan-1-one (4)
Within 30 min, 10.6 g (265 mmol, 3.00 mol eq) NaOH in 303 mL H2O was added to a solution of 40.9 g (88.5 mmol, 1.00 mol eq) 3 in a mixture of solvents (82 mL of MeOH and 151 mL of H2O). The mixture was stirred overnight at rt. The brown precipitate was filtered off, and washed sequentially with 100 mL H2O and 100 mL Et2O and dried under reduced pressure. A yield of 20.11 g (52.7 mmol, 60%) of 4 was obtained and used without further purification in the next step. Its m.p., 1 H-NMR, and IR spectra are described in the literature [26]

Synthesis of 2-Bromo-1-(pyridin-4-yl)ethan-1-one hydrobromide (2)
A solution of 32.0 g (264 mmol, 1.00 mol eq) 1-(pyridin-4-yl)ethan-1-one (1) in 250 mL glacial acetic acid (100%) was cooled in an ice bath, and 150 mL conc. HBr (48 w/w %) was carefully added. Subsequently, a solution of 42.2 g (264 mmol, 1.00 mol eq) Br 2 in 20 mL glacial acetic acid was added dropwise to the reaction mixture. The reaction was stirred overnight at rt while a white precipitate formed. Afterwards, 250 mL Et 2 O was added, and the mixture was stirred for 30 min at rt and filtered. The obtained solid material was washed with 150 mL Et 2 O, dried to yield 70.0 g (249 mmol, 94%) of 2 in the form of a white solid powder. Salt 2 was used in the next step without further purification. Its m.p., 1 H-NMR [24] and MS [25] spectra are described in the literature.

Synthesis of (2-Oxo-2-(pyridin-4-yl)ethyl)triphenylphosphonium bromide (3)
First of all, 65.4 g (249 mmol, 1.00 mol eq) PPh 3 and 34.7 mL (25.2 g, 249 mmol, 1.00 mol eq) Et 3 N were sequentially added to a solution of 70.0 g (249 mmol, 1.00 mol eq) 2 in 400 mL of THF. The reaction mixture was refluxed overnight while an orange precipitate formed. After cooling the mixture to rt, the solid material was filtered off, washed with 150 mL Et 2 O, and dried under reduced pressure to yield 95.9 g (207 mmol, 83%) of an orange product 3, which was used without further purification in the next reaction.

Synthesis of 1-(Pyridin-4-yl)-2-(triphenyl-λ 5 -phosphanylidene)ethan-1-one (4)
Within 30 min, 10.6 g (265 mmol, 3.00 mol eq) NaOH in 303 mL H 2 O was added to a solution of 40.9 g (88.5 mmol, 1.00 mol eq) 3 in a mixture of solvents (82 mL of MeOH and 151 mL of H 2 O). The mixture was stirred overnight at rt. The brown precipitate was filtered off, and washed sequentially with 100 mL H 2 O and 100 mL Et 2 O and dried under reduced pressure. A yield of 20.11 g (52.7 mmol, 60%) of 4 was obtained and used without further purification in the next step. Its m.p., 1 H-NMR, and IR spectra are described in the literature [26]

Drug Preparation
Stock solutions of the multikinase inhibitor sunitinib L-malate (Pfizer, New York, NY, USA) (abbreviated to sunitinib or SU in the text for simplification) and the glucose metabolism inhibitor (E)-1-(pyridin-4-yl)-3-(quinolin-2-yl)prop-2-en-1-one (PFK15) were prepared in dimethyl sulfoxide (DMSO; Sigma-Aldrich, USA) at a concentration of 10 mM. The stock solutions were subsequently diluted in an appropriate concentration in ECGM (and finally in test solutions containing 1% DMSO) for the in vitro experiments. Sunitinib was obtained as a gift from a pharmaceutical company (Pfizer Inc., New York, NY, USA). The synthesis and exact structure assignments of PFK15 were performed as described above.

IC 50 Evaluation
Cells were seeded in a 96-well plate with serial dilutions of PFK15 and sunitinib (starting at 100 µM solution containing 1% DMSO) at a density of 3 × 10 3 cells/well. The MTS assay was performed according to the manufacturer's instructions, as previously described [8]. The half maximal inhibitory concentration (IC 50 ) value was calculated using Graph Pad Prism 6 software (Graph Pad, San Diego, CA, USA).

Cell Proliferation Assay
The MTS assay is generally used for the quantification of cell proliferation, viability, or cytotoxicity. Cell proliferation was determined by colorimetric MTS assay, according to the manufacturer s instructions. Briefly, cells were seeded in a 96-well plate at a density of 3 × 10 3 cells/well and incubated with serial dilutions of PFK15 and sunitinib for 3 days. After 3 days, 10 µL of yellow MTS (5 mg/mL) (Promega, Madison, WI, USA, CAS: 138169-43-4) was added to each well and cells were incubated for 2-3 h at 37 • C. The absorbances were measured at a wavelength of 490 nm (green color). All determinations were performed in quadruplicate, with three independent experiments.

Migration Assay (Wound Healing Assay)
Cells were seeded in 24-well plates coated with 1.5% gelatin (Sigma-Aldrich, USA) at a density of 5 × 10 5 cells/well (HUVEC). After reaching a confluent monolayer the medium was replaced with a starvation medium (ECGM supplemented with 2% FBS) and cells were incubated for a further 17 h. The confluent cell monolayer was wounded using pipet tips and washed twice with PBS. Subsequently, cells were treated with different doses of inhibitors diluted in ECGM medium. The migration of HUVEC was observed with an Olympus IMT2 inverted optical microscope (Olympus, Tokyo, Japan) and recorded by a Moticam 1000 camera system (Motic Incorporation, Hong Kong) at 0 h and 8 h after treatment. Changes in cell migration were evaluated using the software Motic Images Plus 2.0 PL (Motic Incorporation, Hong Kong).

Statistical Analysis
The results are expressed as a mean ± SEM. Each value represents the average of at least three independent experiments. Statistical analysis was performed using STATISTICA 7.0 (StatSoft Inc., Tulsa, OK, USA) and GraphPad Prism 6 (GraphPad Software, Inc.). Statistical differences among groups were determined by one-way ANOVA followed by Tukey's post hoc test. The value p < 0.05 was considered as significant.

Conclusions
In conclusion, we described the synthesis of the glycolysis inhibitor PFK15 in detail. Our results confirmed the effectivity of PFK15 in combination with sunitinib. Sunitinib is an approved drug with therapeutic applications against tumor growth and tumor angiogenesis. The glycolysis inhibitor PFK15 reduces glucose uptake and causes starvation of activated endothelial and tumor cells. Both tumor and activated endothelial cells are dependent on a high influx of glucose as an important source of energy and building blocks. Therefore, the synergistic effect of the above inhibitors to block different biological targets and mechanisms of action on HUVEC-based angiogenesis is promising from a therapeutical point of view.