Three Microbial Musketeers of the Seas: Shewanella baltica, Aliivibrio fischeri and Vibrio harveyi, and Their Adaptation to Different Salinity Probed by a Proteomic Approach

Kloska, Anna; Cech, Grzegorz M.; Nowicki, Dariusz; Maciąg-Dorszyńska, Monika; Bogucka, Aleksandra E.; Markert, Stephanie; Becher, Dörte; Potrykus, Katarzyna; Czaplewska, Paulina; Szalewska-Pałasz, Agnieszka

doi:10.3390/ijms23020619

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Three Microbial Musketeers of the Seas: Shewanella baltica, Aliivibrio fischeri and Vibrio harveyi, and Their Adaptation to Different Salinity Probed by a Proteomic Approach

Anna Kloska

Grzegorz M. Cech

Dariusz Nowicki

Monika Maciąg-Dorszyńska

³,

Aleksandra E. Bogucka

⁴

^5,6,

^6,7

⁴ and

Agnieszka Szalewska-Pałasz

^2,*

Department of Medical Biology and Genetics, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland

Department of Bacterial Molecular Genetics, University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland

Laboratory of Phage Therapy, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Kładki 24, 80-822 Gdansk, Poland

⁴

Laboratory of Mass Spectrometry-Core Facility Laboratories, Intercollegiate Faculty of Biotechnology UG and MUG, University of Gdansk, Antoniego Abrahama 58, 80-307 Gdansk, Poland

⁵

Pharmaceutical Biotechnology, Institute of Pharmacy, University of Greifswald, Felix-Hausdorff-Str. 3, 17489 Greifswald, Germany

⁶

Institute of Marine Biotechnology, Walther-Rathenau-Str. 49, 17489 Greifswald, Germany

⁷

Department Microbial Proteomics, Institute of Microbiology, University of Greifswald, Felix-Hausdorff-Str. 8, 17489 Greifswald, Germany

Author to whom correspondence should be addressed.

Academic Editor: Naglis Malys

Int. J. Mol. Sci. 2022, 23(2), 619; https://doi.org/10.3390/ijms23020619

Received: 27 November 2021 / Revised: 1 January 2022 / Accepted: 4 January 2022 / Published: 6 January 2022

(This article belongs to the Special Issue Gene Expression Regulation in Microorganisms: Molecular Mechanisms, Systems and Synthetic Approaches)

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Abstract

Osmotic changes are common challenges for marine microorganisms. Bacteria have developed numerous ways of dealing with this stress, including reprogramming of global cellular processes. However, specific molecular adaptation mechanisms to osmotic stress have mainly been investigated in terrestrial model bacteria. In this work, we aimed to elucidate the basis of adjustment to prolonged salinity challenges at the proteome level in marine bacteria. The objects of our studies were three representatives of bacteria inhabiting various marine environments, Shewanella baltica, Vibrio harveyi and Aliivibrio fischeri. The proteomic studies were performed with bacteria cultivated in increased and decreased salinity, followed by proteolytic digestion of samples which were then subjected to liquid chromatography with tandem mass spectrometry analysis. We show that bacteria adjust at all levels of their biological processes, from DNA topology through gene expression regulation and proteasome assembly, to transport and cellular metabolism. The finding that many similar adaptation strategies were observed for both low- and high-salinity conditions is particularly striking. The results show that adaptation to salinity challenge involves the accumulation of DNA-binding proteins and increased polyamine uptake. We hypothesize that their function is to coat and protect the nucleoid to counteract adverse changes in DNA topology due to ionic shifts. View Full-Text

Keywords: osmotic stress; proteomic analysis; marine bacteria; Shewanella baltica; Vibrio harveyi; Aliivibrio fischeri

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Kloska, A.; Cech, G.M.; Nowicki, D.; Maciąg-Dorszyńska, M.; Bogucka, A.E.; Markert, S.; Becher, D.; Potrykus, K.; Czaplewska, P.; Szalewska-Pałasz, A. Three Microbial Musketeers of the Seas: Shewanella baltica, Aliivibrio fischeri and Vibrio harveyi, and Their Adaptation to Different Salinity Probed by a Proteomic Approach. Int. J. Mol. Sci. 2022, 23, 619. https://doi.org/10.3390/ijms23020619

AMA Style

Kloska A, Cech GM, Nowicki D, Maciąg-Dorszyńska M, Bogucka AE, Markert S, Becher D, Potrykus K, Czaplewska P, Szalewska-Pałasz A. Three Microbial Musketeers of the Seas: Shewanella baltica, Aliivibrio fischeri and Vibrio harveyi, and Their Adaptation to Different Salinity Probed by a Proteomic Approach. International Journal of Molecular Sciences. 2022; 23(2):619. https://doi.org/10.3390/ijms23020619

Chicago/Turabian Style

Kloska, Anna, Grzegorz M. Cech, Dariusz Nowicki, Monika Maciąg-Dorszyńska, Aleksandra E. Bogucka, Stephanie Markert, Dörte Becher, Katarzyna Potrykus, Paulina Czaplewska, and Agnieszka Szalewska-Pałasz. 2022. "Three Microbial Musketeers of the Seas: Shewanella baltica, Aliivibrio fischeri and Vibrio harveyi, and Their Adaptation to Different Salinity Probed by a Proteomic Approach" International Journal of Molecular Sciences 23, no. 2: 619. https://doi.org/10.3390/ijms23020619

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Three Microbial Musketeers of the Seas: Shewanella baltica, Aliivibrio fischeri and Vibrio harveyi, and Their Adaptation to Different Salinity Probed by a Proteomic Approach

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