Novel Dopamine Transporter Inhibitor, CE-123, Ameliorates Spatial Memory Deficits Induced by Maternal Separation in Adolescent Rats: Impact of Sex

Maternal separation (MS) is a key contributor to neurodevelopmental disorders, including learning disabilities. To test the hypothesis that dopamine signaling is a major factor in this, an atypical new dopamine transporter (DAT) inhibitor, CE-123, was assessed for its potential to counteract the MS-induced spatial learning and memory deficit in male and female rats. Hence, neonatal rats (postnatal day (PND)1 to 21) were exposed to MS (180 min/day). Next, the acquisition of spatial learning and memory (Barnes maze task) and the expression of dopamine D1 receptor, dopamine transporter (DAT), and the neuronal GTPase, RIT2, which binds DAT in the vehicle-treated rats were evaluated in the prefrontal cortex and hippocampus in the adolescent animals. The results show that MS impairs the acquisition of spatial learning and memory in rats, with a more severe effect in females. Moreover, the MS induced upregulation of DAT and dopamine D1 receptors expression in the prefrontal cortex and hippocampus in adolescent rats. Regarding RIT2, the expression was decreased in the hippocampus for both the males and females, however, in the prefrontal cortex, reduction was found only in the females, suggesting that there are region-specific differences in DAT endocytic trafficking. CE-123 ameliorated the behavioral deficits associated with MS. Furthermore, it decreased the MS-induced upregulation of D1 receptor expression level in the hippocampus. These effects were more noted in females. Overall, CE-123, an atypical DAT inhibitor, is able to restore cognitive impairment and dopamine signaling in adolescent rats exposed to MS—with more evident effect in females than males.


Introduction
Early life experiences are thought to play a key role in brain function and behavior [1]. Experimental and clinical studies have shown that the immaturity and plasticity of the central nervous system (CNS) during childhood make it particularly sensitive to stress at a young age, which may cause significant changes in brain structure and function [1]. In humans, exposure to early life stress has been linked to a variety of affective and addictive disorders in later life [2][3][4][5], and to cognitive disorders such as learning and memory deficits [6,7].
The maternal separation (MS) protocol is a well-known animal model that resembles the stress of early life negative experiences and is considered an analog of childhood important role in intracellular signaling and interacts with DAT in lipid raft microdomains: this interaction is required for protein kinase C (PKC)-regulated DAT trafficking in the nervous system [51]. Additionally, published data described the region-and sex-dependent differences in DAT trafficking regulation by RIT2 [52].
The aim of the present study was to determine whether MS (PND1-21, 180 min/day) affects the development of spatial memory (Barnes maze task) in rats and whether this effect is sex dependent. Using ELISA assays, we evaluated MS-induced changes in the DAT, dopamine D1 receptor expression and RIT2 level in the prefrontal cortex and hippocampus. Finally, we determined whether CE-123, a novel atypical DAT inhibitor, has an impact on the learning acquisition impaired by MS in adolescent rats.

Effect of CE-123 on Acquisition of Spatial Learning of the Barnes Maze in Young Male and Female Rats with Prior MS
Male: We examined the deleterious effect of MS on spatial memory acquisition in adolescent male rats. Acquisition of spatial learning in the training phase was evaluated by the decrease in latency time to reach the escape box for four days (PND30-33). A two-way ANOVA with repeated measures showed the significant effect of day of acquisition learning (F(3,216) = 101.0; p < 0.001) and the group of rat effect (F(5,216) = 7.157; p < 0.001), but no interaction between these factors (F(15,216) = 0.9264; p > 0.05) in young male rats (PND30-33). Moreover, the post-hoc Tukey's test demonstrated that maternal separation increased primary latency at days 3rd and 4th. Moreover, pre-treatment with CE-123 at the dose of 5 and 10 mg/kg decreased the primary latency on the 3rd (p < 0.05) day of the acquisition learning trial, while the day 4th only dose 10 mg/kg decreased the primary latency in comparison to the MS group (p < 0.05) ( Figure 1A). In the number of primary errors committed by the rats, a two-way ANOVA with repeated measures indicated the significant effect of day of acquisition learning (F (3,216) = 214.8; p < 0.001) and group of rats (F(5,216) = 7.69; p < 0.001), but no interaction between these two factors in acquisition learning (F(15,216) = 1.177; p > 0.05) in young male rats (PND30-33). The post-hoc Tukey's test demonstrated significant increase in number of errors in the MS group at days 3rd and 4th. Furthermore, pre-treatment with CE-123 at the dose of 10 mg/kg significantly decreased the number of primary errors on the 3rd (p < 0.01) and 4th day (p < 0.05) of the acquisition learning trial ( Figure 1B). Thus, CE-123 decreased the deleterious effect of MS on the acquisition of spatial memory in adolescent male rats.
Female: We studied the deleterious effect of MS on spatial memory acquisition in adolescent female rats. A two-way ANOVA with repeated measures showed the significant effect of day of acquisition learning (F(3,216) = 78.78; p < 0.001) and group of rat effect (F(5,216) = 12.17; p < 0.001), but no interaction between these factors (F(15,216) = 0.5267; p > 0.05) in young female rats (PND30-33). Moreover, the post-hoc Tukey's test demonstrated that MS increased the primary latency in the Barnes maze task acquisition at days 2, 3 (p < 0.01), and 4 (p < 0.05). In addition, pre-treatment with CE-123 at the dose of 10 mg/kg decreased the primary latency on the 2nd (p < 0.01), 3rd, and 4th day (p < 0.05) of the acquisition learning trial ( Figure 1C). In the number of primary errors committed by the rats, a two-way ANOVA with repeated measures indicated significant effect of day of acquisition learning (F(3,216) = 94.22; p < 0.001) and group of rats (F(5,216) = 13.82; p < 0.001), but no interaction between these two factors in acquisition learning (F(15,216) = 0.8899; p > 0.05) in young female rats (PND30-33). The post-hoc Tukey's test demonstrated that MS increased the number of errors at days 2 (p < 0.001), 3 (p < 0.01), and 4 (p < 0.05). Moreover, pre-treatment with CE-123 at the dose of 5 mg/kg significantly decreased the number of primary errors on the 2nd day (p < 0.05) of the acquisition learning trial ( Figure 1D). In addition, the post-hoc Tukey's test demonstrated that pre-treatment with CE-123 at the dose of 10 mg/kg significantly decreased the number of primary errors on the 2nd (p < 0.01), 3rd (p < 0.01) and 4th days (p < 0.05) of the acquisition learning trial ( Figure 1D). Overall, CE-123 strongly ameliorated the MS-induced deficits on the acquisition of spatial memory in adolescent female rats.
Female: We studied the deleterious effect of MS on spatial memory acquisit adolescent female rats. A two-way ANOVA with repeated measures showed the s cant effect of day of acquisition learning (F(3,216) = 78.78; p < 0.001) and group of rat (F(5,216) = 12.17; p < 0.001), but no interaction between these factors (F(15,216) = 0.5 > 0.05) in young female rats (PND30-33). Moreover, the post-hoc Tukey's test d strated that MS increased the primary latency in the Barnes maze task acquisition a 2, 3 (p < 0.01), and 4 (p < 0.05). In addition, pre-treatment with CE-123 at the dose mg/kg decreased the primary latency on the 2nd (p < 0.01), 3rd, and 4th day (p < 0 the acquisition learning trial ( Figure 1C). In the number of primary errors commit the rats, a two-way ANOVA with repeated measures indicated significant effect of acquisition learning (F(3,216) = 94.22; p < 0.001) and group of rats (F(5,216) = 13.8 0.001), but no interaction between these two factors in acquisition learning (F(15 0.8899; p > 0.05) in young female rats (PND30-33). The post-hoc Tukey's test demons that MS increased the number of errors at days 2 (p < 0.001), 3 (p < 0.01), and 4 (p < Moreover, pre-treatment with CE-123 at the dose of 5 mg/kg significantly decreas Figure 1. Effect of CE-123 (5 mg/kg and 10 mg/kg) or vehicle given each day before the acquisition session on the primary latency for males (A) and females (C) and number of errors for males (B) and females (D) committed during 4 days of the acquisition of spatial learning of the Barnes maze task in adolescent (PND30-33) rats. Wistar rats were subjected to MS for 180 min during PND1-21. Data represent mean ± SEM (N = 10 rats/group). * p < 0.05, ** p < 0.01, *** p < 0.001 Stressed/Vehicle vs. Nonstressed/Vehicle; # p < 0.05 Stressed/CE-123 5 mg/kg vs. Stressed/Vehicle; $ p < 0.05, $$ p < 0.01; Stressed/CE-123 10 mg/kg vs. Stressed/Vehicle; PND-postnatal day.

Effect of CE-123 Given during Acquisition Session on Spatial Memory Retention in the Probe-Trial of the Barnes Maze in Young Male and Female Rats with Prior MS
Male: The probe-trial was conducted 24 h after the last acquisition session. Two-way ANOVA with repeated measures indicated the significant effect of MS (F(1,54) = 10.65; p < 0.01), CE-123 administration (F(2,54) = 7.218; p < 0.01) and interaction between these factors (F(2,54) = 9.436; p < 0.001). Tukey's test revealed that MS increased primary latency as compared to nonstressed rats (p < 0.001) and CE-123 given each day before acquisition sessions at the dose of 10 mg/kg decreased this effect (p < 0.001) ( Figure 2A). Additionally, these factors (MS (F(1,54) = 12.01; p < 0.01); CE-123 (F(2,54) = 4.622; p < 0.05); interaction (F(2,54) = 3.761; p < 0.05)) had significant effects on the number of errors committed by rats during the probe-trial. Post-hoc test showed that MS increased the number of errors (p < 0.01) when compared to nonstressed rats. CE-123 given before every acquisition session at the dose of 10 mg/kg counteracted this MS-induced effect (p < 0.01) ( Figure 2B), suggesting that CE-123 increased memory retention in MS male rats.

Hippocampus
Two-way ANOVA with repeated measures indicated a significant effect of MS

The Influence of CE-123 Administration before Each Acquisition Session on D1 Receptor Expression Level in the Hippocampus of MS Male and Female Adolescent Rats
Two-way ANOVA with repeated measures indicated the significant effect of CE-123 at the dose of 10 mg/kg administered before each acquisition session (F(1,16) = 45.75; p < 0.001), but not sex factor (F(1,16) = 1.049; p > 0.05), on D1 receptor expression in the hippocampus of MS male and female rats. Interaction between these two factors was significant (F(1,16) = 6.427; p < 0.05). Tukey's post-hoc test revealed that CE-123 administration decreased D1 receptor expression in both male (p < 0.05) and female (p < 0.001) ( Table 1) MS rats, with a greater effect upon the females. Table 1. Effect of CE-123 administration (10 mg/kg) before each acquisition session on D1 receptor expression in the hippocampus of MS male and female adolescent (PND34) rats. Data represent mean ± SEM (N = 5 rats/group). The concentration of proteins was calculated from a standard curve and expressed as ng/mg of protein. * p < 0.05; *** p< 0.001 vs. stressed rats.

Discussion
Our results indicate that MS impairs the acquisition of spatial learning and memory in both male and female adolescent rats. The MS animals needed more time than the controls and made more errors in reaching the escape box during the acquisition phase of spatial learning in the Barnes maze task. These deleterious effects of MS on spatial learning and memory were more severe in female than male rats. CE-123, a DAT inhibitor, was found to improve learning in the MS rats. Notably, it was found to reduce latency and number of errors, with more evident effect in females. The proteomic analysis further supports the behavioral results, suggesting that MS upregulated DAT and dopamine D1 receptors expression in the prefrontal cortex and hippocampus. Regarding RIT2, the expression was decreased in the hippocampus for both the males and females, however, in the prefrontal cortex, reduction of expression was found only in the females, with no changes in the RIT2 expression in males. Overall, our data indicate that the deleterious effect of MS on learning and memory in adolescent rats may be due to hypofunction of dopamine signaling.
Evidence suggests that chronic early life stress has harmful effects on cognitive functions such as learning and memory in humans [7] and animals [53,54] in later life. Preclinical data reveal that, for example, MS [55] during the neonatal period (PND2-14, 180 min/day) reduces spatial memory formation in adult male rats (40 and 70 weeks old), indicating long term disruptive effects on brain structure and behavior. Another study shows that MS (PND2-5, 240 min/day, and then PND6-16, 480 min/day) with early weaning (PND17) slightly impairs working memory during non-stressful situations in female rats, but does not change spatial reference memory or associative learning under stressful circumstances in either sex [56]. In turn, our previous study demonstrated that MS (PND1-21, 180 min/day) induced deficits in the acquisition of spatial memory in the Barnes maze task in adolescent rats (PND30-33), with no significant differences between males and females [57]. However, in that study, data were pooled over 4 days of the memory development.
In the current study, when learning was analyzed each day of training, we found that the daily MS (PND1-21, 180 min/day) schedule indeed impaired spatial memory development in adolescent rats of both sexes (PND30-33), however, the female rats were more vulnerable to MS than males. Herein, the female MS rats (but not male MS rats) made significantly more errors and required longer time intervals to reach the escape box during the Day 2 acquisition phase than the controls. Published data confirmed that MS (24 h at PND3) affects hippocampal structural plasticity in adolescent (PND21) rats in a sex-dependent manner, and previous work demonstrated that neurogenesis is significantly increased in male, but decreased in female offspring after MS [58,59]. Accordingly, female rats (exposed to 24 h of MS at PND3) exhibited a lower total number of mature granule cells in adulthood [58], potently limiting the number of synaptic contacts that can be established in this region. Other research [60] indicates that the deleterious effects of MS on learning and memory are clearly age-and sex-dependent, but also rely on duration of the manipulation. However, we found that not only female, but also male rats showed deficits in learning procedure. In our study, a significant primary latency delay was observed in these animals on the 3rd and 4th day of the acquisition phase of the Barnes maze task. Such effects may suggest that male rats with MS experience exhibited significant impairment in retention of spatial information.
Studies in a variety of species have revealed that the development of the dopaminergic system is particularly sensitive towards prenatal stress [61,62] and neonatal stress experience [62][63][64][65][66][67]. Dopamine D1-like receptors in the hippocampus are especially noted for their critical role in spatial memory [27,28], and activating these receptors could enhance spatial learning and memory [25]. More recently, it has been reported that D1/D5 receptor signaling is associated with long-term memory consolidation [29] and working memory [30]. Thus, dopamine D1 receptor activation in the hippocampal regions can enhance memory and modulate memory consolidation or retrieval [31][32][33]68] through, for instance, modulation of excitatory amino acids (EAAs) release [68] and induction of long-term potentiation (LTP).
Our present study showed that the expression of dopamine D1 receptors was upregulated in the hippocampus and prefrontal cortex of MS rats. Such effects were more pronounced in female than in male rats. Thus, our results suggest reduced dopamine transmission at the dopamine D1 receptors in adolescent rats induced by prolonged neonatal MS. Additionally, in previous work, the upregulation of D1 receptors has been observed in the hippocampus of adult rats after 15 min MS [4]. Other authors also have demonstrated that MS (24 h on PND9) increases the expression of dopamine D1 (and dopamine D2) receptors [69] in such brain structures as the prefrontal cortex and striatum in rats. However, further studies have indicated that chronic stress produces an increase in D1 receptor density in the prefrontal cortex, and drugs with dopamine-related actions improve spatial working memory in these subjects [22,70]. These findings suggest that an early life MS may alter the developmental trajectory of dopamine receptors, and this may have functional consequences on behavior in later life.
DAT is a membrane-bound presynaptic protein that rapidly clears dopamine that has been released into the extracellular space, and thereby limits the amplitude and duration of dopamine signaling. Previous studies have revealed reduced DAT expression in the midbrain and striatum of prenatally stressed mice [71] or upregulation of striatal DAT in rhesus monkeys [72]. Our study indicated upregulation of DAT in MS animals in the hippocampus and prefrontal cortex. This effect was most evident in the hippocampus of female rats. Our findings support a body of literature suggesting that prefrontal and hippocampal dopamine dysfunction might contribute to the cognitive and behavioral impairments reported in children from prenatally stressed pregnancies. An example of prenatal stress in children is attention deficit hyperactivity disorder (ADHD) [73]. Although results are mixed [74,75], ADHD patients are commonly treated with the DAT inhibitor, methylphenidate [76], although other DAT inhibitors, such as modafinil [77], were also clinically tested in ADHD.
CE-123, as we previously stated, is a modafinil analog with increased affinity and selectivity for DAT [46,47]. In the current study, CE-123 (5 and 10 mg/kg) enhanced the acquisition of memory in the Barnes maze task in male and female adolescent rats (PND30-33) exposed to MS (PND1-21, 180 min/day) during the neonatal period. The effects of CE-123 were more pronounced in females than in males. The most effective dose was 10 mg/kg CE-123. This dose decreased latency and number of errors in both sexes of rats. Furthermore, CE-123 given during the memory acquisition phase had a beneficial effect on memory retention in the probe-trial test. The effects were more significant in females than in males. Together, our results support a functional link between dopaminergic neurotransmission and learning disabilities in rats with MS in the neonatal period, especially in females.
DAT surface availability is dynamically regulated by endocytic trafficking, and direct PKC activation acutely diminishes DAT surface expression by accelerating DAT internalization. Previous cell line studies demonstrated that PKC-stimulated DAT endocytosis requires both Ack1 (a non-receptor tyrosine kinase) inactivation, which releases a DATspecific endocytic brake, and the neuronal GTPase, RIT2, which binds DAT. However, it is unknown whether RIT2 is required for PKC-stimulated DAT endocytosis in dopaminergic terminals or whether there is region-and/or sex-dependent differences in PKC-stimulated DAT trafficking. Moreover, the mechanisms by which RIT2 controls PKC-stimulated DAT endocytosis are unknown [52].
Our results revealed that MS much more strongly decreased RIT2 expression in the hippocampus in female than in male rats. However, in the prefrontal cortex, MS decreased RIT2 only in the females, whereas RIT2 protein levels were unaffected in males. Thus, lack of changes in RIT2 expression in the prefrontal cortex in male rats can suggest sex and region-specific differences in DAT endocytic trafficking. Considering our outcomes, we hypothesize that the downregulation of RIT2 expression implies a lower control under PKC-stimulated DAT endocytosis in these brain structures.
However, our experiments have a limitation. Since DAT blockers can be considered as indirect dopamine receptor agonists and published data have shown that CE-123 administration in rats increased total DAT levels and D1 proteins levels in the synaptosomal fraction of hippocampal subregions CA1 and CA3 [47], we evaluated only the impact of CE-123 on upregulation of D1 receptors (but not DAT) in the hippocampus of MS rats. Herein, our results showed that CE-123 decreased the D1 receptor upregulation, as we revealed in Table 1. Furthermore, although published data indicated close relationships between RIT2 and D1 receptors in male striatal function, as well as dopamine-dependent behavior [78], we did not assess the influence of CE-123 on RIT2 expression. Thus, our data only partially supports the parallelism between the behavior and the biomarkers. This subject will be addressed in a future study.
Collectively, our experiments indicate that MS induced deficits in the acquisition of learning and memory in the Barnes maze test in both groups of adolescent rats: male and female. The effects were more pronounced in female than in male rats. Thus, we suggest that females are more vulnerable to cognitive impairments that are induced by mother neglect during the neonatal period. Moreover, neonatal MS induces hypofunction of dopaminergic transmission in adolescent rats (with DAT overexpression and upregulation of D1 receptors) that is decreased by CE-123, an atypical DAT inhibitor (as indicated in our preliminary study in the hippocampus), leading to amelioration of the spatial memory impairment in MS rats. Hence, CE-123 administration could inhibit DAT upregulation, normalize dopamine signaling at the D1 receptor, and effectively improve learning and memory in MS rats. This is the first study that reveals the potential usefulness of DAT inhibitors, such as CE-123, in restoring spatial memory deficits induced by MS. However, more research is needed.

Animals
The study was approved by the Local Ethics Committee

Drugs
S-CE-123 (5-((benzhydrylsulfinyl)methyl) thiazole) (CE-123), a modafinil analog, was synthesized in the Lubec laboratory (University of Vienna, Vienna, Austria) as reported previously [46]. On the day of the experiments, CE-123 was freshly dissolved in a vehicle of 1% DMSO and 3.3% Tween 80 diluted in 0.9% NaCl. CE-123 was administered intraperitoneally (i.p.) at the doses of 5 or 10 mg/kg in a value of 1 mL/kg of body weight 30 min prior to each acquisition session. The CE-123 doses were selected based on previous research [46][47][48]. Controls were administered by vehicle alone.

Maternal Separation
The maternal separation (MS) procedure was carried out from PND1 to PND21. The total number of dams used in the present study was 20 (10 litters for MS stress and 10 litters for control). The procedure of MS was based on the protocol described by Chocyk et al. [79] with minor modifications. On each of PND1-21, the dams and the pups were removed from the maternity cages for 180 min (09:00 to 12:00). The mothers were placed individually in holding cages, while each litter was placed in a cardboard container lined with fresh bedding material. The containers were moved to a bigger cage where adequate ambient temperature (34 • C) was provided by placing hot water bottles on the bottom. After the 180 min separation, the pups and the dams were returned to the maternity cages. Once a week, the maternity cages were cleaned during one of the separation procedures. Control, non-separated (NS) animals were left undisturbed with their mothers, except during the cage cleaning that was performed once a week. During the MS procedure, male and female segregation was not performed. After weaning, offspring at PND 22 were separated according to sex, housed 5 per cage, and assigned to the Barnes maze task (PND29). Male and female offspring were used in the present study (N = 10 rats/group).

Barnes Maze Task
The Barnes maze task was carried out according to the method described in our earlier study [80]. The Barnes maze apparatus (Stoelting, Dublin, Ireland) consisted of a circular grey metal platform (diameter 122 cm), elevated 100 cm above the floor, with 20 holes (10 cm diameter) located in its periphery. One hole was connected to an escape box of 35 cm × 12 cm × 12 cm of the same material and color as the platform. The other holes were covered underneath with a flat box, also of the same material and color, so that the rats could not discriminate the escape hole from other holes until situated adjacent to it. In addition, numerous visual cues (in the form of large colorful geometric shapes) were placed on the walls of the testing room at 1-2 m distance from the edge of the maze. To evoke the potentiated escape response, the platform was brightly lit (two points of light 1.5 m above the maze: 500 W each) as an aversive stimulus. The Barnes maze task was run in two phases: habituation and acquisition trial.

Habituation
On PND29, one day before the acquisition phase, to reduce anxiety behavior, the rats were habituated to the platform and the escape box. This habituation trial was performed with the lights on.

Acquisition Phase
One day (24 h) after the maze habituation, the same rats were subjected to the acquisition phase (PND30-33). Acquisition involved one training session per day for 4 consecutive days. Each training session consisted of two 180 s trials, with a 5-min inter-trial interval when the animals were returned to their home cages. CE-123 at the doses of 5 or 10 mg/kg was administered once daily 30 min before every first acquisition trial for 4 days of the acquisition phase. The location of the platform and the escape box remained constant over all the acquisition trials. Each trial began by placing the animal at the center of the platform and then it was allowed to freely explore the apparatus. The trial was completed after 180 s or when the animal entered the escape box. Immediately after entering the escape box, the hole was covered for 30 s before the rat was returned to its home cage. If the animal did not enter the goal box within 180 s, it was gently guided there by the experimenter and could explore it for 30 s. To dissipate odor cues and to provide a standard olfactory context for each trial, the platform surface and the escape box were wiped with a 10% (w/v) ethanol solution after each trial [80,81]. All trials were recorded by a trained observer. Since the animals occasionally lacked motivation and merely explored the maze after finding the escape box rather than entering it, following the work of many authors [82][83][84][85], in our experiments, we scored such parameters as the primary latency and primary errors. Primary latency was defined as the time required for the rat to make initial contact with the escape box. Primary errors were defined as the number of holes visited before the first contact with the escape box.

Probe-Trial
To evaluate spatial memory retrieval, 24 h after the acquisition phase, the subjects (PND34) received a probe-trial for 180 s. During this trial, the rats were allowed to explore the maze. The primary latency and primary errors to reach the escape box were counted. On the day of the probe trial, no substances were administered to the animals.

ELISA Assays
Quantitative measurement (PND33) of dopamine receptor 1 (D1), dopamine transporter (DAT) and GTP-binding protein RIT2 in such brain structures as the prefrontal cortex and hippocampus was performed using a Rat D1 receptor ELISA kit (Reddot Biotech, Kelowna, BC, Canada), a Rat Dopamine Transporter (DAT) ELISA kit (E0222Ra; Bioassay Technology Laboratory, Shanghai, China) and Rat RIT2 (GTP-Binding Protein Rit2) ELISA Kit (ELK9277; ELK Biotechnology, Wuhan, China), respectively, following manufacturers' protocols. Firstly, frozen rat brain structures were homogenized in ice-cold PBS pH 7.4 containing cocktails of protease and phosphatase inhibitors (Sigma-Aldrich, Saint Louis, MO, USA) using a homogenizer ball (Bioprep-24, Aosheng, Hangzhou, China) (10 s at 10,000 rpm). Then, homogenates were centrifuged for 5 min at 5000× g and the supernatants were immediately removed. Duplicates of each sample and series of standards were transferred to ELISA plates. The absorbance was measured at a wavelength of λ = 450 nm using a Multiskan Spectrum spectrophotometer (Thermo LabSystems, Philadelphia, PA, USA). The concentration of proteins was calculated from a standard curve and expressed as ng/mg of protein. For protein measurement, the bicinchoninic acid assay (BCA) protein assay kit (Serva, Heidelberg, Germany) was used. ELISA assays were performed after completion of the Barnes maze task in the vehicle-and CE-123-treated animals.

Statistical Analysis
The obtained results were analyzed using Prism v. 8.0.0 for Windows (GraphPad Software, San Diego, CA, USA). The statistical significance of drug effects from behavioral and biochemical tests was analyzed by the two-way analysis of variance (ANOVA) with repeated measures. This was followed by Tukey's post-hoc test. The results were presented as means ± standard errors of means (SEM) of values. p value less than 0.05 was considered statistically significant for all tests.