CRISPR-Based Genome Editing and Its Applications in Woody Plants

CRISPR/Cas-based genome editing technology provides straightforward, proficient, and multifunctional ways for the site-directed modification of organism genomes and genes. The application of CRISPR-based technology in plants has a vast potential value in gene function research, germplasm innovation, and genetic improvement. The complexity of woody plants genome may pose significant challenges in the application and expansion of various new editing techniques, such as Cas9, 12, 13, and 14 effectors, base editing, particularly for timberland species with a long life span, huge genome, and ploidy. Therefore, many novel optimisms have been drawn to molecular breeding research based on woody plants. This review summarizes the recent development of CRISPR/Cas applications for essential traits, including wood properties, flowering, biological stress, abiotic stress, growth, and development in woody plants. We outlined the current problems and future development trends of this technology in germplasm and the improvement of products in woody plants.


Introduction
Woody plants pertain to long-term plants with highly lignified stems, many lignin cells, and moderately complex and upright stems. They are structurally defined as macrophanerophytes, bushes, and/or subshrubs appearing in different shapes. It has been proved that woody plants are assets for human health, building structure, energy, industry, landscape, food, and pharmaceuticals. Woody plants are vital in biodiversity, surface morphology, and worldwide climate [1]. Therefore, it is critical to improve the environmental resistance, production, and other traits of woody plants through genetic engineering technology to cultivate new higher-quality varieties.
Compared with seasonal herbs, woody plants have many interesting natural characteristics: Long life span, extended growth period, vast and complex genome, indefinite hereditary basis, and complex regeneration capacity [2]. Previously, woody plant breeding methods were carried out through cross-breeding techniques [3]. However, the process was slow and lengthened the production cycle. Up-to-date, modern genetic engineering breeding methods are efficient and shorten the breeding and production cycles through DNA manipulation [4,5]. Therefore, these methods are essential for modern breeding and research work in woody plants. There are remarkable advances in woody plant genetic engineering, such as biotic and abiotic stress resistance [6] and trait improvement [7]. As a core of conventional genetic engineering for plant breeding technology, transgenic technology has improved germplasm and allowed gene regulation expression analyses of target genes in plants [8,9].
Nonetheless, precise target genome editing cannot be achieved in woody plant breeding due to the problems discussed below [10]. Recently, scientists overcame the barriers of precise genome editing in poplar using optimized transformation efficiency to replace exogenous BleoR [11].
plants [29][30][31], and new gene editors have been developed based on the CRISPR/Cas technique for genome editing promotion.

New Editors Based on CRISPR/Cas 2.2.1. CRISPR/Cas12
The type V CRISPR/Cas12 system is a comprehensive defense system with up to 11 identified subtypes. Cas12a, also known as Cpf1, is a type V-A CRISPR/Cas system that is closely similar to Cas9. Xu et al. [32] first used the CRISPR/Cas12a in rice to edit the OsPDS and OsBEL genes, successfully mutating these two targets. Subsequently, Cpf1 has been widely used in plant genome editing since it does not depend on remote DNA and maintains a strategic distance from genome contamination by foreign DNA. The Cas12b CRISPR system protein (C2c1) is a double-RNA-directed DNA endonuclease of the type V-B CRISPR/Cas system, with amino acids ranging from 1100 to 1500 and smaller than Cpf1 and Cas9 proteins [33]. However, Cas12b was unsuitable for genome editing due to its thermophilic characteristics. Cas12b requires a combination of crRNA and tracrRNA to form sgRNA targeting DNA and is more responsive to modification than Cas12a [34]. Recently, Wu et al. [35] have used two endonucleases, BvCas12b and BhCas12b v4, to generate mutant Arabidopsis through multiple gene editing and produced large deletions at multiple sites. However, Cas12b has a shallow off-target rate and the potential for more precise genome editing than Cas9 [36].

CRISPR/Cas13
The type VI CRISPR/Cas system (CRISPR/Cas13) has been utilized for RNA cleavage, and Cas13a was the first RNA nuclease to be applied [37]. Abudayyeh et al. [38] found that the RNA targeting system could inhibit endogenous genes in protoplasts of Oryza sativa cultured in vitro. However, Cas13a has a low off-target rate compared with the traditional RNAi technology, which both have a similar RNA knockout strain to target the RNA in the nucleus. Cas13a has been applied recently in plants against viruses, such as Turnip Mosaic Virus (TuMV) and Potato virus Y (PVY) [39,40]. Moreover, the non-specific RNA restriction enzyme activity attached to the activation of Cas13a is applied to the rapid detection of nucleic acid and is used in agribusiness, character detection, problem monitoring, and distinguishing between pathogens in crop breeding [41]. The CRISPR/Cas13 may be summarized as a novel CRISPR system targeting RNA, with high targeting efficiency, involved in RNA detection, edition, knockout, tracking, and imaging [37]. The CRISPR/Cas13 system is beneficial because of its reduced operational genomic damages, regulating RNA to change the expression effect of the target gene. Therefore, it is more manageable and efficient than the traditional CRISPR gene editing techniques, such as RNAi. Cas13, as an RNA editing tool, has broad potential applications and prospects.

CRISPR/Cas14
CRISPR/Cas14a is a compact nucleic corrosive protease that targets single-stranded-DNA (ssDNA) cleavage. It mainly binds and cleaves the target ssDNA under the direction of sgRNA. The Cas14 protein has a small amino acid length (400-700 aa) and does not require a PAM sequence. Similar to the Cas12 protein, Cas14a binds to target nucleic acid, resulting in a trans-cleavage activity. Contrary to the Cas12 protein, CRISPR/Cas14a only binds to ssDNA targets. Therefore, amplified and enriched target nucleic acids need to be treated with T7 nucleic acid exonuclease, such as amplification with phosphothiolation cation primers to ensure that only one strand of the T7 exonuclease is cleaved. CRISPR/Cas14a is an ideal tool for combating plant ssDNA viruses [42].

Base Editing (BE)
Base editing combines the targeting function of specific DNA sequences recognized by the CRISPR/Cas system with the biochemical functions of adenine-and-cytosine deaminase to replace a single base in a target DNA sequence. Depending on the type of deaminase combined, base editing systems can be classified as cytosine base editors (CBE) and adenine base editors (ABE) [43]. The CBE ( Figure 1E) system completes the single base replacement of the target site C-T (G-A): The ABE ( Figure 1F) system completes a single base substitution of A-G (T-C) at the target site. Plant base editor (PBE) is a base editing system in which BE3 is applied for successful plant genome editing. Zong et al. [44] used the PBE system to edit the bases of protoplasts of crops, and the new single base editing system A3A-PBE enabled more efficient C-T single base editing in wheat, rice, and potatoes PBE [45]. The base editing system targets the desired genomic DNA (gDNA) site with no double-stranded DNA break. It uses deaminase to the target site to realize accurate single-base substitution. However, this system has disadvantages, such as PAM sequence limitation, grouping foundation reliance, and off-target impact [46]. with phosphothiolation cation primers to ensure that only one strand of the T7 exonuclease is cleaved. CRISPR/Cas14a is an ideal tool for combating plant ssDNA viruses [42].

Base Editing (BE)
Base editing combines the targeting function of specific DNA sequences recognized by the CRISPR/Cas system with the biochemical functions of adenine-and-cytosine deaminase to replace a single base in a target DNA sequence. Depending on the type of deaminase combined, base editing systems can be classified as cytosine base editors (CBE) and adenine base editors (ABE) [43]. The CBE ( Figure 1E) system completes the single base replacement of the target site C-T (G-A): The ABE ( Figure 1F) system completes a single base substitution of A-G (T-C) at the target site. Plant base editor (PBE) is a base editing system in which BE3 is applied for successful plant genome editing. Zong et al. [44] used the PBE system to edit the bases of protoplasts of crops, and the new single base editing system A3A-PBE enabled more efficient C-T single base editing in wheat, rice, and potatoes PBE [45]. The base editing system targets the desired genomic DNA (gDNA) site with no double-stranded DNA break. It uses deaminase to the target site to realize accurate single-base substitution. However, this system has disadvantages, such as PAM sequence limitation, grouping foundation reliance, and off-target impact [46].

CRISPR-Based Delivery Systems in Woody Plants
The CRISPR genome editing system requires an efficient delivery system to achieve satisfactory results [47]. One of the biggest obstacles in applying CRISPR-based gene editing in woody plants is the lack of an efficient delivery system. The current delivery methods mainly rely on agrobacterium-mediated transformation ( Figure 2; Table 1). Agrobacteriummediated transformations pose many advantages, such as low cost, developing stable transgenic plants by integrating the desired foreign DNAs, and carrying multiple binary vectors into the plant cells. However, Agrobacterium tumefaciens can contaminate many plant tissues, damaging the plant materials during the transformation process. ment, leaving a staggering end in dsDNA (Provided by BioRender.com; accessed on 14 March 2022).

CRISPR-Based Delivery Systems in Woody Plants
The CRISPR genome editing system requires an efficient delivery system to achieve satisfactory results [47]. One of the biggest obstacles in applying CRISPR-based gene editing in woody plants is the lack of an efficient delivery system. The current delivery methods mainly rely on agrobacterium-mediated transformation ( Figure 2; Table 1). Agrobacterium-mediated transformations pose many advantages, such as low cost, developing stable transgenic plants by integrating the desired foreign DNAs, and carrying multiple binary vectors into the plant cells. However, Agrobacterium tumefaciens can contaminate many plant tissues, damaging the plant materials during the transformation process.  Table 1). In Hevea brasiliensis, the PEG was used in a delivery-mediated system of Cas protein and sgRNA pre-assembled into a dynamic CRISPR/Cas ribonucleoprotein complex (RNP) in vitro into plant protoplasts with no exogenous DNA expansions [48]. Moreover, this method has been applied to apples and grapes [49,50]. However, it has not been extensively used in woody plants because of the limitations in protoplast preparation, transformation, and regeneration techniques. Similar to the PEG method, liposomes could mediate protoplast transformation by encapsulating RNP and delivering it to the protoplasts through endocytosis, resulting in a more efficient process than PEG [51]. Therefore, establishing and improving the protoplast regeneration system of woody plants will accelerate the genome-editing-mediated breeding process. Both delivery methods require a tissue culture system, as mentioned above. The polyethylene glycol (PEG)-mediated delivery method has been applied to the woody plant genome editing system, which can realize exogenous DNA-free cells ( Figure 2; Table 1). In Hevea brasiliensis, the PEG was used in a delivery-mediated system of Cas protein and sgRNA pre-assembled into a dynamic CRISPR/Cas ribonucleoprotein complex (RNP) in vitro into plant protoplasts with no exogenous DNA expansions [48]. Moreover, this method has been applied to apples and grapes [49,50]. However, it has not been extensively used in woody plants because of the limitations in protoplast preparation, transformation, and regeneration techniques. Similar to the PEG method, liposomes could mediate protoplast transformation by encapsulating RNP and delivering it to the protoplasts through endocytosis, resulting in a more efficient process than PEG [51]. Therefore, establishing and improving the protoplast regeneration system of woody plants will accelerate the genome-editing-mediated breeding process. Both delivery methods require a tissue culture system, as mentioned above.
Plant genome editing can be achieved by de novo induction of meristem. Maher et al. [52] utilized the fast-processed agrobacterium coculture (Fast-TrACC) to deliver the developmental regulators WUS2, IPT, STM, and sgRNA into tobacco somatic cells to produce shoots with targeted gene modifications. It is known that growth hormones induce the formation of new meristem development, leading to the formation of buds with targeted DNA modifications, which produces transgenic sprouts in plants, including tomatoes, potatoes, and woody grapes. Research has evidenced that de novo induction of the meristem sidesteps the tissue culture process to perform plant gene editing.
First, particle bombardment is commonly used in plant gene editing systems, transmitting genetic material through rigid cell walls by a mechanical force [53] (Figure 2; Table 1). However, it is less efficient than agrobacterium-mediated methods and may also cause the rupture of foreign genes and the destruction of genomic sequences during bombardment [54][55][56]. However, this method is yet to be reported in woody plants.
Plant virus vectors have also been utilized for in vivo delivery and transient expression of foreign genes ( Figure 2). Hu et al. [57] designed a barley stripe mosaic virus (BSMV)based gRNA delivery system for CRISPR/Cas9-mediated targeted mutagenesis in wheat and corn with efficiencies of 78% and 48%, respectively. Ma et al. [58] used an engineered plant minus-strand RNA elastomeric vector to deliver efficient, stable, heritable targeted gene editing CRISPR/Cas9 endonuclease in heterologous tetraploid tobacco. This technique is advantageous since the edited plants will not contain exogenous DNA fragments because the RNA virus genome is not integrated into the host plant genome.
Nanomaterials have also been used to deliver CRISPR vectors into plants ( Figure 2). Doyle et al. [59] coated GFP, Cas9, and gRNA with carbon dots (CDs) and sprayed the CDs carrying the Cas9 plasmid on wheat leaves to target the SPO11 gene. Presumably, the carbon nanotubes as new nanomaterials can deliver DNA to plant cells, such as tobacco, wheat, and cotton, and transient gene expression. This method is expected to be applied in gene editing and avoid vector fragmentation [60].

Timber Properties of Woody Plants
As a renewable biological material, wood is a necessity for human life. The formation and thickening of the secondary cell wall (lignin and cellulose deposition) are critical steps in wood formation and growth. Wood has many excellent properties and various uses. The CRISPR/Cas system has provided the functional study of genes relation and wood properties of woody plants [107].
The content of lignin accounts for about 25% of woody plants and is important in forming cell walls, providing strength and hydrophobicity to woody tissues. The CRISPR/Cas9 system was utilized to knock out the 4CL1 and 4CL2 genes from Populus with up to 100% mutation efficacy [61]. The mutant plants had a 23% reduction in lignin content, resulting in decreased lignin monophenol content. Therefore, the mutant material with a reddish-brown stem was obtained, and these results showed that the CRISPR/Cas9 system is efficient in Populus. In the monolignol synthesis pathway, CAFFEOYL SHIKIMATE ESTERASE (CSE) converts caffeoyl shikimate into caffeic acid. De Vries et al. [62] knocked out the CSE1 and CSE2 genes of poplar (Populus tremula × P. alba) by CRISPR/Cas9 and obtained the cse1 cse2 double mutants, whose lignin content decreased by 35%, accompanied by growth restriction. The results showed that the CSE gene was an important target for improving the wood properties of woody plants. The R2R3 MYB transcription factor regulatory network can affect the formation of a secondary wall in plants. Yang et al. [63] applied CRISPR/Cas9 to knock out PtoMYB156, which encoded the R2R3-MYB transcription factor in Populus tomentosa. Their results demonstrated that lignin, xylan, and cellulose had ectopic deposition during the formation of the secondary cell wall. It was found that Pto-MYB156 negatively regulated the formation of the secondary cell wall of Populus tomentosa.
Tension wood (TW) is a particular xylem tissue developed and formed under mechanical or tensile stress. Its development involves the trans-regulation of secondary cell wall genes to alter wood properties and environmental adaptability. Liu et al. [64] induced TW in the stems of black cottonwood (Populus trichocarpa, Nisqually-1) and identified two significantly repressed transcription factor (TF) genes: Class B3 heat-shock TF (HSFB3-1) and MYB092. They knocked out PtrHSFB3-1 and PtrMYB092 genes by CRISPR/Cas9, which condensed the mutant lignin and high cellulose content. These results verified that these TFs played a regulatory role in the biosynthesis of cell wall components during TW formation.
Brassinosteroids (BRs), as plant steroid hormones, play a regulatory role in xylem development. DWF4 gene is a related gene in brassinosteroid biosynthesis. Shen et al. [65] knocked out the PtoDWF4 gene of Populus tomentosa using CRISPR/Cas9, which reduced the lignin content by 8.79-11.67%. Their results demonstrated that PtowDWF4 positively regulates xylem development during wood formation. The study analyzed the critical gene for xylem development in poplar, laying a solid foundation for cultivating poplar varieties with high-yield wood in the future. Moreover, DET2 is an important rate-limiting enzyme in the BR biosynthesis pathway. Fan et al. [66] improved the bioethanol yield of lignocellulose from Populus tomentosa by regulating brassinosteroid biosynthesis. The secondary cell wall of the PtoDET2 mutant produced by CRISPR/Cas9 became thinner, and the ratio of cellulose crystallinity index (CRI), degree of polymerization (DP), and hemicellulose increased. These findings showed that PtoDET2 had a positive regulatory effect on xylem development, cell wall biosynthesis, and modification of poplar. The VNS gene family plays a critical regulatory role in secondary wall synthesis. Takata et al. [67] knocked out four VNS genes of Populus tremula L. × Populus tremuloides Michx. using the CRISPR/Cas9, and secondary cell walls were not formed in xylem wood fibers, xylem ray parenchyma cells, and phloem fibers in the four mutants of vns09/vns10/vns11/vns12, but only some wood fibers near the vessel element showed secondary cell wall deposition, indicating that VNS genes in Populus coordinated the regulation of the formation of secondary cell walls.

Flower Development of Woody Plants
Flowering is necessary to regulate the reproductive and genetic abilities of higher plants. This trait is delimited by genes and a complex molecular genetic network. To reach the flowering stage, woody plants undergo a long juvenile period. The CRISPR-based gene editing technology can be used to study the genes related to woody flowering, flowering traits, nutrition regulations, and the reproductive growth of woody plants to improve yield or shorten the breeding cycle.
The FLOWERING LOCUS T (FT) protein is the flowering factor signal for flowering initiation, while the TERMINAL FLOWER1 (TFL1) protein acts as an anti-flowering pigment to inhibit flowering. Fan et al. [48] knocked out the HbFT and HbTFL1 genes in Hevea brasiliensis by CRISPR/Cas9 and obtained plants with delayed and early flowers. Charrier et al. [68] knocked out the TFL1 gene of pear and apple by CRISPR/Cas9. Their re-sults disclosed that 93% and 9% of the apple and pear mutant lines, respectively, show early flowering. Kiwifruit is dioecious during a long juvenile period. CENTRORADIALIS (CEN)like genes have been identified as potential flowering repressors. Varkonyi-Gasic et al. [69] knocked out the AcCEN4 and AcCEN genes of kiwifruit A. chinensis by CRISPR/Cas9, leading to early mutant blooming and the production of more compact fruit. The above research results based on the CRISPR/Cas9 gene editing system enable the creation of new forest germplasm with diverse flower development cycles and the shortening of breeding cycles of perennial woody plants.

Improvement of Stress Resistance
Biotic and abiotic factors reduce the development and growth of woody plants. The CRISPR-based gene editing system enabled the breeding of resistant varieties of woody plants.

Biotic Stress
Melampsora can cause poplar rust disease, one of the most widely distributed and most harmful diseases of poplar. Salicylic acid-mediated pathways are essential for plant defense against pathogens. WRKY is one of the salicylic acid response transcription factors. Jiang et al. [70] used the CRISPR/Cas9 system to knock out the genes of PtrWRKY18 and PtrWRKY35 in P. trichocarpa and then inoculated Melampsora. Severe symptoms appeared on the leaves of the mutant plants. The results support the SA-mediated signal participation in poplar Melampsora resistance. Cassava brown streak virus is an RNA virus that causes the cassava brown streak disease, and it restricts the growth of plants. Diseases caused by this virus require the interaction between viral genome-linked protein (VPg) and host eukaryotic translation initiation factor 4E (eIF4E). Gomez et al. [71] used CRISPR/Cas9 to edit cassava eIF4E isoforms ncbp-1 and ncbp-2 genes and obtained ncbp-1 and ncbp-2 single and double mutants. After inoculation with the cassava brown streak virus, it was found that the incidence of cassava brown streak disease was significantly reduced. The disease symptoms were weakened in the double mutant plants. Phytophthora is a type of plant pathogenic bacteria, mostly a facultative parasite. Fister et al. [72] used CRISPR/Cas9 to knock out the TcNPR3 gene of the Theobroma cacao, and the resistance of the mutant to the Phytophthora tropicalis infection was enhanced.
Botrytis cinerea is a fungal disease that can attack flowers, fruits, leaves, and stems. VvWRKY52 gene has been proved to play a role in biological stress response. Wang et al. [73] used CRISPR/Cas9 technology to knock out the VvWRKY52 gene of grape (Vitis vinifera). The inoculation experiment of Botrytis cinerea indicated that the mutant strain remarkably improved the resistance to Botrytis cinerea, and the double allele mutant strain had increased resistance than the single allele mutant strain. Grapevine downy mildew is a significant oomycete disease caused by Plasmopara viticola and is harmful to grape production. Pathogenesis-related (PR) proteins comprise a large class of plant disease defense proteins. Li et al. [74] used CRISPR/Cas9 to knock out VvPR4b gene in grapes. The mutant strain was more sensitive to Plasmopara viticola, which proved that VvPR4b has the disease resistance to Plasmopara viticola in grapes.
Xanthomonas citri subspecies citri (Xcc) is the main pathogenic factor of citrus canker, and CsLOB1 is the susceptible gene of citrus canker. Jia et al. [75] used CRISPR/Cas9 to edit the PthA4 effect binding element in the type I CsLOB1 gene promoter in Duncan grapefruit, and the mutant showed slight relief from citrus canker symptoms. Peng et al. [76] edited the CsLOB1 gene promoter in Wanjincheng orange (Citrus sinensis Osbeck) using the CRISPR/Cas9 technique. Moreover, mutant lines showed high resistance to citrus canker. The results prove that the CRISPR/Cas9-mediated promoter editing of CsLOB1 is an efficient strategy for generating canker-resistant citrus cultivars. Jia et al. [77] used the newly developed CRISPR/Cas12a editor in the CsLOB1 gene of Duncan grapefruit. Here, mutant plants with resistance to citrus canker were obtained, and the resulting biallelic mutant probability was lower than CRISPR/Cas9 editing. In addition to editing the CsLOB1 gene, Wang et al. [78] used CRISPR/Cas9 to knock out the CsWRKY22 gene in Wanjincheng orange (Citrus sinensis (L.) Osbeck), reducing the susceptibility to canker disease. All the above results are helpful in cultivating new woody varieties with disease resistance.
Herbicides are potentially harmful to woody plants when used to control the weed. Therefore, the prime needs to develop herbicide-resistant assortments. Acetolactate synthase (ALS) confers resistance to the herbicide chlorsulfuron. Malabarba et al. [79] used base editing to create an amino-acid substitution on ALS genes in apple and pear, which resulted in the non-binding of the herbicide chlorsulfuron. Thus, the mutant obtained resistance to the herbicide chlorsulfuron.

Abiotic Stress
Drought, high salt, and other environmental factors affect the growth and yield of woody plants. WRKY gene plays a vital role in regulating the response of woody plants to salt stress. Jiang et al. [80] used CRISPR/Cas9 to mutate the PalWRKY77 gene of Populus alba var. pyramidalis, which increased the salt tolerance of poplar plants. Suggesting that Populus can recruit the abscisic acid (ABA) signaling pathway to adapt to a saline environment. Additional research revealed that PalWRKY77 negatively regulates the ABA-responsive gene and relieves ABA-mediated growth inhibition, which causes Populus to be more sensitive to salt stress. AREB1-ADA2b-GCN5 ternary protein complex can be used for drought response and tolerance of Populus species by coordinating histone acetylation and gene activation, which is mediated by transcription factors. Li et al. [81] used CRISPR/Cas9 to knock out the PtrADA2b-3 gene of Populus trichocarpa, significantly reducing drought tolerance in mutant plants. Nuclear factor Y (NF-Y) transcription factor is important in regulating plant growth, development, and stress response. Zhou et al. [82] used CRISPR/Cas9 to knock out the PdNF-YB21 gene of Populus, decreasing the root growth of the mutant plants and drought tolerance. In contrast, overexpression of PdNF-YB21 in Populus promoted root growth and improved drought resistance, evidencing that the NF-Y transcription factor plays an essential role in the drought resistance of poplar.

Secondary Metabolism
Plants create abundant secondary metabolites that regulate growth and development, increase resistance, enhance regeneration components, and increase nutritional ability.
Carotenoids are involved in the light-harvesting stage of photosynthesis and antioxidant functions in woody plants and provide excellent color to stems, leaves, flowers, fruits, and other organs [108]. Phytoene dehydrogenase (PDS) is the first rate-limiting enzyme in the carotenoid synthesis pathway. It has been reported that a mutant with an albino phenotype was obtained by knocking out the PDS gene using the CRISPR/Cas9 and Cas12 systems, demonstrating the vital role of the PDS gene in the carotenoid synthesis pathway. The CRISPR/Cas9 technology was applied to knock out the PDS gene to produce albino plants in sweet orange [83], poplar [84], apple [85], grape [86], cassava [87], citrus [88], coffee [89], cotton [90], green bamboo [91], walnut [92], and other species. Moreover, An et al. [93] used the CRISPR/Cas12 to knock out the PDS gene of poplar and obtained albino phenotypic plants.
Anthocyanin, a water-soluble natural pigment in plants, participates in the development and defense of plants. Proanthocyanins are polyphenols, and anthocyanins are flavonoids. During heat, the procyanidins produce anthocyanins in an acidic medium. Wang et al. [94] knocked out the poplar MYB115 gene by CRISPR/Cas9, and the accumulation of procyanidins in mutants decreased. The results indicated that the MYB115 gene was involved in the positive regulation of procyanidins biosynthesis. Wan et al. [95] knocked out the PtrMYB57 gene of Populus tomentosa with CRISPR/Cas9 system, resulting in increased anthocyanin and proanthocyanidin in the plant. JMJ25 is a histone H3K9 demethylase gene. Fan et al. [96] knocked out the JMJ25 gene of Populus by CRISPR/Cas9 system, which encodes histone H3K9 demethylase. The mutant strain produced ectopic anthocyanin accumulation and increased the anthocyanin biosynthesis gene expression.
The salicylate is broadly covered within the bark and removes different varieties of salix in poplar plants, which may play a crucial part in resisting the herbivores. UDPglucose-dependent glycosyltransferase (UGT) is a widespread enzyme in plants that plays several roles in secondary plant metabolism, hormonal, and other small molecule detoxification. Fellenberg et al. [97] knocked out the UGT71L1 gene related to the synthesis of salicylate from poplar by CRISPR/Cas9, resulting in a partial reduction in salicylate content. Pomegranate is rich in phenolic metabolites. Chang et al. [98] used CRISPR/Cas9 to knock out the two UDP-glycosyltransferase genes PgUGT84A23 and PgUGT84A24 in pomegranate (Punica granatum L.), thus reducing the content of phenolic substance angoranin in pomegranate. Ren et al. [99] knocked out the tartaric acid (TA) synthesis key gene IdnDH in grapes using CRISPR/Cas9, which remarkably reduced the TA content in the mutant and confirmed the feasibility of applying the technology to grape genome transformation.
Caffeine is a plant alkaloid, and its anabolism in tea is regulated by the N-methyltransferase gene (NMT), an essential enzyme gene. Ma et al. [100] cloned CsHB1 from tea plant (Camellia sinensis (L.) o. Kuntze) by a single yeast hybrid using yhNMT1 as bait. Using CRISPR/Cas9 technology, the CsHB1 gene was effectively mutated in transgenic tea callus. This reduced the expression of CsHB1 by 65%, downregulating the expression of N-methyltransferase gene yhNMT1, which catalyzes caffeine synthesis in tea. Consequently, this reduced the caffeine content in callus and its caffeine accumulation by 97.5%. This study enhanced the understanding of the regulatory mechanism of caffeine biosynthesis in tea.

Growth and Development
Plant hormones can affect physiological processes, such as germination, rooting, and fruiting, and play an important role in regulating the growth and development of plants. Common hormones include auxin, gibberellin, cytokinin, abscisic acid, ethylene, brassinosteroids, and strigolactone. Cai et al. [101] knocked out the cytokine synthesisrelated gene JcCYP735A of Jatropha curcas by CRISPR/Cas9, decreasing the cytokinin content in leaves. The plants indicated apparent growth retardation, and the height was only a quarter of the wild plants. Ye et al. [102] knocked out a gibberellin response gene GRG1 in hexaploid Ma bamboo (Dendrocalamus latiflorus Munro) by CRISPR/Cas9, resulting in a significant increase in plant height. It is of great significance to study the rapid elongation and growth of Gramineae bamboo.
Tropical tree Parasponia andersonii has the ability of nodulation and nitrogen fixation. Zeijl et al. [103] knocked out PanHK4, PanEIN2, PanNSP1, and PanNSP2 genes of Parasponia andersonii using the CRISPR/Cas9, which control cytokinin, ethylene, or strigolactone hormone networks, respectively. They found that the PanNSP1 and PanNSP2 genes are crucial for nodule formation, while PanHK4 mutation leads to a decrease in the cambium activity of stem, and PanEIN2 mutation leads to gender differentiation.
Strigolactone (SLs) is a newly discovered plant hormone that can inhibit branching in woody plants. CCD7 and CCD8 are SL biosynthesis genes. Ren et al. [104] edited VvCCD7 and VvCCD8 genes in Vitis vinifera using CRISPR/Cas9. The ccd8 mutant had more branches than the corresponding wild-type plant, and the results emphasized the critical role of VvCCD8 in controlling grape stem branches. The TCP-type transcription factors BRANCHED1 and BRANCHED2 shape the plant architecture by suppressing bud outgrowth. Muhr et al. [105] used the CRISPR/Cas9 system to knock out the transcription factors PcBRC1-1 and PcBRC2-1 of Populus. The branches in the mutant plant significantly increased, and the pcbrc2-1 demonstrated a higher bud growth rate. Arginine in plants has the function of storing nitrogen nutrition. Wang et al. [106] knocked out two GhARG homologous genes in upland cotton by CRISPR/Cas9, which inhibited the arginase activity. Nonetheless, they activated nitric oxide synthase activity, inducing arginine to synthesize more nitric oxide (NO) and promoting the formation of lateral roots, thereby potentially promoting the growth of the whole cotton plant and finally increasing the fiber yield. To summarize the manipulations of the CRISPR/Cas system in woody plants discussed above, Figure 3 below sheds light on the key features of the CRISPR/Cas gene editing.
factors PcBRC1-1 and PcBRC2-1 of Populus. The branches in the mutant plant signifi-cantly increased, and the pcbrc2-1 demonstrated a higher bud growth rate. Arginine in plants has the function of storing nitrogen nutrition. Wang et al. [106] knocked out two GhARG homologous genes in upland cotton by CRISPR/Cas9, which inhibited the arginase activity. Nonetheless, they activated nitric oxide synthase activity, inducing arginine to synthesize more nitric oxide (NO) and promoting the formation of lateral roots, thereby potentially promoting the growth of the whole cotton plant and finally increasing the fiber yield. To summarize the manipulations of the CRISPR/Cas system in woody plants discussed above, Figure 3 below sheds light on the key features of the CRISPR/Cas gene editing.

Conclusions
Forest trees have significant financial and environmental benefits within human society; therefore, it is crucial to carry out germplasm development and gene editing on woody plants. The CRISPR/Cas system has been manipulated to investigate crucial characteristics of woody plants, such as wood properties, blooming, natural product quality, and resistance. These are prime focal points in forestry breeding, trait development and alteration, and quality enhancement. With the improvement and innovation of CRISPR/Cas gene editing technology, some other proficient and accurate gene editing systems are constantly emerging. The above-discussed researches illustrate the application of the CRISPR/Cas9 technology, using current gene editing techniques in woody plants. Certain newly developed editors, such as single base editing and CRISPR/Cas12, are also applied, and further novel editors will be applied in woody plants in the future, such as CRISPR/Cas13, CRISPR/Cas14, and prime editor. However, there is still a need to improve the application of these tools in woody plants. Broadening the application of these modern advances in woody plants will encourage the acceleration of the qualitative research process and advancement of assorted woody plants. CRISPR/Cas system knocks out plant genome and creates gene additions and deletions at the specific site. The effectiveness of target inclusion and substitution primarily involves constraining its application in plant genome research and breeding.

Conclusions
Forest trees have significant financial and environmental benefits within human society; therefore, it is crucial to carry out germplasm development and gene editing on woody plants. The CRISPR/Cas system has been manipulated to investigate crucial characteristics of woody plants, such as wood properties, blooming, natural product quality, and resistance. These are prime focal points in forestry breeding, trait development and alteration, and quality enhancement. With the improvement and innovation of CRISPR/Cas gene editing technology, some other proficient and accurate gene editing systems are constantly emerging. The above-discussed researches illustrate the application of the CRISPR/Cas9 technology, using current gene editing techniques in woody plants. Certain newly developed editors, such as single base editing and CRISPR/Cas12, are also applied, and further novel editors will be applied in woody plants in the future, such as CRISPR/Cas13, CRISPR/Cas14, and prime editor. However, there is still a need to improve the application of these tools in woody plants. Broadening the application of these modern advances in woody plants will encourage the acceleration of the qualitative research process and advancement of assorted woody plants. CRISPR/Cas system knocks out plant genome and creates gene additions and deletions at the specific site. The effectiveness of target inclusion and substitution primarily involves constraining its application in plant genome research and breeding.
Establishing a more efficient technical system for inserting and replacing plant genome fragments is urgent. CRISPR/Cas9 technology has a broad application prospect, but there are still particular shortcomings, such as PAM sequence dependence and distance and editing efficiency. The off-target phenomenon can be reduced using the appropriate Cas protein or improving the specificity of the sgRNA sequence.
In woody plants, gene editing innovation inquiries primarily employ agrobacteriummediated or PEG-mediated change innovation as a delivery system. Due to the confinement of the natural characteristics of different woody plants, these delivery systems are limited to a few plant species or tissues. The strategy of inducing meristem from scratch based on Fast-TrACC has comprehended the editing of plant genes in grapes, avoiding tissue culture.
It is expected to develop further into a new strategy to overcome the bottleneck of editing plant genes. At the same time, the established gene gun method and virus vector delivery method have exposed their high efficiency and convenience in the gene editing system of model plants and herbs. Nonetheless, a mature technical system must be established in woody plants, especially forest trees. The nanomaterial-based delivery system can combine CRISPR/Cas systems to create genetically engineered plants without transgene integration, while selecting and realizing efficient delivery according to the characteristics of nanomaterials. The above-discussed delivery strategies are predicted to be the future development trend of plant gene editing, but whether they are equally applied to woody plants remains to be confirmed by research.
Compared with herbaceous plants, CRISPR/Cas system is still in the primary stage of establishing a gene editing system in most woody plants. There are still many difficulties and challenges in applying CRISPR/Cas system to woody plants. The main reasons are as follows: First, woody plant genomes are huge, highly heterozygous, and repetitive. Second, there are few woody plants with completed genome sequencing at present, and the genomic data of forest species recorded in the target site design and analysis platform is not perfect, which leads to problems, such as the inability to design gRNA. Finally, most woody plants have not yet established a stable, efficient, and time-consuming genetic transformation system. Developing protoplasts of woody plants to plan tissue culture recovery systems and progressing the effectiveness of single-cell change will help accelerate the era of highquality strains by gene editing. It is expected that with the research of genes related to imperative characteristics and infections of woody plants, gene editing innovation based on CRISPR/Cas will be an essential device for genetic engineering research in woody plants.