Anti-Inflammatory Activity of Cajanin, an Isoflavonoid Derivative Isolated from Canavalia lineata Pods

The bioactive components of Canavalia lineata (Thunb.) DC pods were investigated using bioactivity-guided isolation, and the chemical structures of flavonoids 1–3, isoflavonoid derivatives 4–11, and phenolic compounds 12 and 13 were identified by comparing NMR, MS, and CD spectral data with previously reported spectroscopic data. Compounds 1–13 were evaluated for their anti-inflammatory effects on LPS-stimulated RAW264.7 macrophages. Among these compounds, the isoflavonoid derivative cajanin (7) exhibited the most potent anti-inflammatory activity (IC50 of NO = 19.38 ± 0.05 µM; IC50 of IL-6 = 7.78 ± 0.04 µM; IC50 of TNF-α = 26.82 ± 0.11 µM), exerting its anti-inflammatory effects by suppressing the activation and nuclear translocation of the transcription factor NF-κB by phosphorylating IκB and p65. These results suggested that cajanin (7) may be a potential candidate for improving the treatment of inflammatory diseases.


Introduction
Canavalia lineata (Thunb.) DC. belongs to the genus Canavalia, which comprises approximately 51 species in the family Fabaceae, representing sword beans (Canavalia gladiata) and jack beans (Canavalia ensiformis) [1,2]. Canavalia lineata is a vine-type perennial plant that inhabits China, Japan, Taiwan, and the coastal region of Jeju Island in Korea. The mature beans are known to contain beneficial proteins and bioactive compounds [3], and the brown seeds grow to approximately 1.5 cm in pods 5-10 cm long and 3.0-3.5 cm wide. Canavalia lineata has been known to be a risk factor for fetal death in pregnant women and is traditionally used on Jeju Island to prevent pregnancy. The Korean name "Haenyeo-kong", which means female diver bean, originates from its traditional use involving contraception [4,5].
The term homeostasis is usually defined as a physiologically balanced condition. In contrast, the term inflammation is often used to describe a response to an infection or injury caused by an extrinsic or intrinsic stimulus, which leads to an abnormal pathological condition due to a loss of homeostatic capacity [6]. Although the inflammatory response leads to a temporary disruption in homeostasis, the inflammatory process ultimately restores physiological homeostasis by eliminating pathogens such as microbes, fungi, and viruses [6,7]. However, dysregulated inflammation is the cause of inflammatory diseases, including allergies, asthma [8], rheumatoid arthritis [9], and sepsis [10]. Inflammatory signals are transferred indirectly or directly, depending on the particular cell type, to initiate or suppress certain core functions. For instance, macrophages contribute to a variety of roles, including wound healing, angiogenesis, tissue development, and vital innate immune responses to infectious pathogens [11,12]. However, the production of large amounts of inflammatory cytokines and mediators, such as nitric oxide (NO), tumor necrosis factor α (TNF-α), and interleukin-6 (IL-6), is induced by macrophage cells in response to adverse signs and can contribute to pathological inflammatory processes through signal transduction pathways, including the nuclear factor kappa-light-chainenhancer of activated B cells (NF-κB) pathway [13,14]. Thus, restoring homeostasis from unregulated inflammatory processes by regulating these core pathways should be an effective approach for the treatment of inflammatory diseases.
C. lineata has been reported to be used not only as a female natural contraceptive, but also as livestock feed [4,5]; however, few studies have reported on the metabolites of C. lineata. Therefore, a phytochemical study on the composition of C. lineata may be applicable to the utilization of these natural resources as industrial crops. In the present study, the phytochemical components of the C. lineata pod methanol extract were purified by bioactivity-guided chromatographic separation to produce 13 compounds, and their chemical structures were elucidated based on NMR and MS spectroscopic data. Among these compounds 1-13, cajanin (7), an isoflavonoid compound, exhibits a higher antiinflammatory effect than that of other isolates by effectively inhibiting the inflammatory signaling mechanism and NF-κB pathways.
. 2022, 23, x FOR PEER REVIEW   The aglycone (3) and sugar of compound 2 were produced by the hydrolysis of compound 2 with 10% HCl, and the sugar derivative of compound 2 and D-, L-glucose and D-, L-galactose standards were analyzed using UPLC-QTOF-MS. The identity of the sugar of compound 2 was determined by comparing its retention time with those of the authentic sugar derivative peaks, which was verified by the mass fragmentation pattern of hexose derivatives, namely, m/z 455, 433, 320, 298, 262, 178, 160, and 136 [26]. The retention time (t R : 15.9 min) of the sugar derivative obtained from compound 2 corresponded to that of D-glucose (t R : 15.8 min, Figure S10). Therefore, the sugar of compound 2 was determined to be D-glucose, and the structure of compound 2 was elucidated as (2R,3R)-3-hydroxy 7-O-β-D-glucopyranoside-6-methoxyflavanone.

Anti-Inflammatory Effects of Compounds 4 and 7 through Inhibition of NF-κB Activat
NO is catalyzed by the activation of nitric oxide synthase (NOS), which has th lowing isoforms: endothelial NOS (eNOS), neuronal NOS (nNOS), and inducible (iNOS) [29]. eNOS and nNOS are constitutively expressed by endothelial cells in the culature and neurons in the nervous system, respectively, and relatively small amo of NO are secreted by both eNOS and nNOS enzymes. However, extensive amoun NO produced by iNOS, which is only activated in response to specific inflammator nals, such as microbial or inflammatory mediators, are implicated in a variety of in matory diseases [30]. A Western blot analysis was performed to evaluate the inhib effects of prunetin (4) and cajanin (7) on iNOS protein expression in LPS-activated m  (7) on the inflammatory mediators NO (A), IL-6 (B), and TNF-α (C) in LPS-stimulated RAW264.7 macrophages. NO, IL-6, and TNF-α secretion was measured by NO and ELISAs as described in the Materials and Methods. Cytotoxicity was not observed at the IC 50 concentrations of prunetin (4) and cajanin (7) (D), and all data are expressed as the mean ± SEM of three independent experiments. Statistical significance was considered at * p < 0.05 compared to the LPS-only treatment group. 2-Amino-4-methylpyridine (AMP) and dexamethasone (Dex) were employed as positive controls.

Anti-Inflammatory Effects of Compounds 4 and 7 through Inhibition of NF-κB Activation
NO is catalyzed by the activation of nitric oxide synthase (NOS), which has the following isoforms: endothelial NOS (eNOS), neuronal NOS (nNOS), and inducible NOS (iNOS) [29]. eNOS and nNOS are constitutively expressed by endothelial cells in the vasculature and neurons in the nervous system, respectively, and relatively small amounts of NO are secreted by both eNOS and nNOS enzymes. However, extensive amounts of NO produced by iNOS, which is only activated in response to specific inflammatory signals, such as microbial or inflammatory mediators, are implicated in a variety of inflammatory diseases [30]. A Western blot analysis was performed to evaluate the inhibitory effects of prunetin (4) and cajanin (7) on iNOS protein expression in LPS-activated murine macrophages. As shown in Figure 4, cajanin (7) decreased the expression levels of iNOS in a dose-dependent manner, whereas prunetin (4) showed levels that were not significantly different from the comparison group treated with LPS alone. Therefore, compared to prunetin, cajanin (7) is a more effective than compound (4) for inhibiting the protein levels of iNOS that are stimulated by LPS-treated macrophages. These data were consistent with the inhibitory effect of prunetin (4) and cajanin (7) on NO production ( Figure 3). levels of iNOS that are stimulated by LPS-treated macroph sistent with the inhibitory effect of prunetin (4) and cajanin (7 3). The inflammatory molecules iNOS, NO, TNF-α, and ILscription factor NF-κB, a key mediator of inflammatory r whether the activity of NF-κB was suppressed by cajanin (7) phorylation of IκB (p-IκB) and p-p65 in whole-cell lysates wa analysis. As shown in Figure 5A, compared to prunetin (4), ca the expression of p-IκB and p-p65 at 12.5 and 25 µM compare LPS alone. In addition, an immunofluorescence analysis was blockade effect of nuclear transcription factor (NF-κB) affected (4). As shown in Figure 5B, cajanin (7) dramatically diminish κB (p65) to the nucleus at 25 µM, whereas prunetin (4) failed to location of NF-κB (p65) to the nucleus at this same concentra sults suggested that the inhibitory effects of cajanin (7) on LPS  (7) on LPS-activated iNOS protein expression in macrophages. Western blot analysis was performed to measure iNOS protein levels normalized to that of β-actin as the loading control. The ratio of the iNOS/β-actin band intensities was calculated using the ImageJ program. The results are shown as the mean ± SEM of three independent experiments. Asterisks indicate a significant difference compared to the LPS alone group (* p < 0.05).
The inflammatory molecules iNOS, NO, TNF-α, and IL-6 are regulated by the transcription factor NF-κB, a key mediator of inflammatory responses [31]. To examine whether the activity of NF-κB was suppressed by cajanin (7) and prunetin (4), the phosphorylation of IκB (p-IκB) and p-p65 in whole-cell lysates was measured by Western blot analysis. As shown in Figure 5A, compared to prunetin (4), cajanin (7) markedly inhibited the expression of p-IκB and p-p65 at 12.5 and 25 µM compared to the group treated with LPS alone. In addition, an immunofluorescence analysis was performed to investigate the blockade effect of nuclear transcription factor (NF-κB) affected by cajanin (7) and prunetin (4). As shown in Figure 5B, cajanin (7) dramatically diminished the translocation of NF-κB (p65) to the nucleus at 25 µM, whereas prunetin (4) failed to completely block the translocation of NF-κB (p65) to the nucleus at this same concentration ( Figure S18). These results suggested that the inhibitory effects of cajanin (7) on LPS-induced inflammatory mediators, such as NO, IL-6, TNF-α, and iNOS, were attributed to the blockade of NF-κB transcription factor activity via the suppression of p-p65 and p-IκB.
x FOR PEER REVIEW 7 of 13 Figure 5. Effect of prunetin (4) and cajanin (7) on the phosphorylation of NF-κB (p65) and IκBα and nuclear translocation of NF-κB (p65) in LPS-induced macrophage cells. (A) Whole-cell lysates were prepared from RAW264.7 cells treated with or without LPS (0.5 µg/mL) and were subjected to Western blot analysis to investigate the phosphorylation of NF-κB (p65) and IκBα. The band intensities of phosphorylation of IκBα (p-IκBα) and NF-κB (p-p65) are represented as fold-change values using ImageJ software compared to total-IκBα (t-IκBα) and total-p65 (t-p65), which was used as a loading control. Data are indicated as the mean ± SEM of three independent experiments. Significant differences are expressed as asterisks between the LPS only and experimental groups (* p < 0.05). (B) Immunocytochemical analysis was performed to explore the nuclear localization of NF-κB (p65) using the fluorescence of Alexa Fluor 488 and Hoechst 33342. Dexamethasone (Dex) served as a positive control.

Discussion
Except for only a few bioactivities, such as antioxidant, anti-inflammatory, and antimelanogenesis effects, the biological activities of C. lineata, which naturally inhabits Jeju  (7) on the phosphorylation of NF-κB (p65) and IκBα and nuclear translocation of NF-κB (p65) in LPS-induced macrophage cells. (A) Whole-cell lysates were prepared from RAW264.7 cells treated with or without LPS (0.5 µg/mL) and were subjected to Western blot analysis to investigate the phosphorylation of NF-κB (p65) and IκBα. The band intensities of phosphorylation of IκBα (p-IκBα) and NF-κB (p-p65) are represented as fold-change values using ImageJ software compared to total-IκBα (t-IκBα) and total-p65 (t-p65), which was used as a loading control. Data are indicated as the mean ± SEM of three independent experiments. Significant differences are expressed as asterisks between the LPS only and experimental groups (* p < 0.05). (B) Immunocytochemical analysis was performed to explore the nuclear localization of NF-κB (p65) using the fluorescence of Alexa Fluor 488 and Hoechst 33342. Dexamethasone (Dex) served as a positive control.

Discussion
Except for only a few bioactivities, such as antioxidant, anti-inflammatory, and antimelanogenesis effects, the biological activities of C. lineata, which naturally inhabits Jeju Island, Korea, have not been fully demonstrated [4,5]. Moreover, few studies have been conducted with the metabolites of C. lineata, with the exception of canavanine, one of the major nonproteinaceous amino acids of the legume family, which is isolated from C. lineata leaves and roots [32,33]. In the current study, the secondary metabolites of C. lineata pods were investigated by chromatographic purification and interpretation of spectroscopic data to generate 1 new flavonoid (2) and 12 known compounds, and their anti-inflammatory activities were explored on LPS-induced inflammatory molecules in RAW264.7 macrophages.
NO has a very short biological half-life in mammalian cells; however, an overproduction of NO is associated with many inflammatory diseases, such as asthma and rheumatoid arthritis. In the field of inflammation studies, this diatomic molecule has been used as a biomarker for bioactive plant extracts or compounds from many natural products [34]. Furthermore, proinflammatory cytokines, including IL-6 and TNF-α, are involved in inflammation-associated diseases and act as inflammatory mediators or activators of inflammatory pathways, such as NF-κB, mitogen-activated protein kinase (MAPK), and the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) cascades [35].
In the search for bioactive metabolites that can reduce the inflammatory molecules that are enhanced by LPS stimulation, the methanol extract (1 g) of C. lineata pods was separated by an MPLC instrument equipped with a C 18 column to yield 14 fractions ( Figure S11). Of the fractions from which water-soluble substances were removed, MPLC fractions 8 and 9 showed an inhibitory effect against LPS-activated NO production without causing cytotoxicity in RAW264.7 cells. (Figures S12 and S13). Based on the UPLC chromatogram (280 nm) of MPLC fractions 8 and 9, in which compounds 3 (t R : 7.45 min), 7 (t R : 8.62 min), 9 (t R : 9.45 min), 4 (t R : 9.98 min), and 8 (t R : 10.18 min) were detected, it was inferred that flavonoid derivatives 3, 4, and 7-9 may contribute to the inhibitory effect of fractions 8 and 9 ( Figure S14). As hypothesized for the biologically active components above, compounds 4, 7, and 9 exhibited a significant inhibitory effect on LPS-activated NO, IL-6, and TNF-α expression (Figures S15 and S16). From the NO bioassay and ELISA IC 50 value results, cajanin (7) showed a 1.9-to 5.2-fold higher inhibitory activity than prunetin (4) and medicarpin (9) (Figure 3 and Figure S17). Based on the differences in the chemical structures of prunetin (4) and cajanin (7), the presence of the OH-2 group in the B ring of cajanin may contribute to its anti-inflammatory activity. In previous studies, prunetin and medicarpin were reported to inhibit LPS-stimulated inflammatory mediator expression by suppressing the activation of the NF-κB pathway [36,37]; however, no studies have reported the anti-inflammatory effects of cajanin (7), which has shown a greater potency than prunetin (4) and medicarpin (9).
In the canonical NF-κB pathway, the NF-κB dimer, composed mainly of p65 (RelA) and p50 subunits, is inactivated by binding to the inhibitor of κB (IκB) proteins, including IκBα and IκBβ, and is sequestered in the cytoplasm. The IκB protein is phosphorylated by the multimeric IκB kinase complex (IKK) and is subsequently degraded by the 26S proteasome. Then, the released NF-κB dimer rapidly relocates from the cytoplasm into the nucleus, leading to the upregulation of the transcriptional activity of inflammatory genes, such as adhesion molecules, inflammatory cytokines, and chemokines [31]. The sustained activation of NF-κB is considered a powerful pathogenic factor that is involved in acute or chronic inflammatory disorders, including chronic obstructive pulmonary disease (COPD), asthma, multiple sclerosis, atherosclerosis, rheumatoid arthritis, and inflammatory bowel disease (IBD) [38]. Therefore, targeting NF-κB signal transduction cascades, including IKK kinase activity, proteasome activity, nuclear translocation, and DNA-binding activity, may be an effective therapeutic strategy for treating inflammatory diseases.
In this study, cajanin, an isoflavonoid isolated from C. lineata pods, significantly suppressed the phosphorylation and nuclear relocation of the transcription factor NF-κB, resulting in a reduction in the production of LPS-stimulated inflammatory molecules such as NO, IL-6, TNF-α, and iNOS. In addition to the NF-κB signaling pathway, our future studies will investigate the anti-inflammatory mechanism of cajanin in more detail.

UPLC-QTOF-MS Analysis for Determining the Sugar Content
In previous studies, the UPLC analysis method coupled with a QTOF-MS detector was employed to determine the absolute configuration of monosaccharide isomers, including hexose, glucose, galactose, allose and pentose, and xylose and arabinose [26]. Depending on our laboratory conditions, the method was modified to analyze the free sugar produced by acid hydrolysis of compound 2. In short, compound 2 (2 mg) was dissolved in 70% EtOH that contained 10% HCl and was refluxed at 90 • C for 2 h. After acid hydrolysis was achieved, the reaction mixture was evaporated in vacuo. The sugar-containing aqueous layer was partitioned with an EtOAc layer and concentrated in a water bath. The dried residue was redissolved in anhydrous pyridine (1 mL, Sigma-Aldrich, St. Louis, MO, USA) and reacted with cysteine methyl ester (6 mg, TCI, Tokyo, Japan) at 60 • C for 1 h. Then, the reaction mixture was incubated with phenyl isothiocyanate (0.1 mL, Sigma-Aldrich) at 60 • C for 1 h, and the final product was analyzed by UPLC-QTOF-MS (Acquity UPLC system, Waters). The UPLC and ESI source conditions were configured according to a previously reported reference [26]. The D-and L-glucose and D-and L-galactose standards (1 mg each, TCI) were analyzed and prepared using the same protocol.

Cell Culture and Viability
RAW264.7 cells were purchased from American Type Culture Collection (ATCC No. TIB-71, Manassas, VA, USA) and cultured in Dulbecco's modified Eagle's medium (DMEM; Welgene, Daegu, South Korea) supplemented with penicillin-streptomycin (100 U/mL) and 10% heat-inactivated fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) at 37 • C in a 5% CO 2 humidified incubator. Cell viability was assayed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reagent, which is reduced by metabolically active cells. The cells were adhered at 1 × 10 4 cells/well in 96-well plates and were treated with compound for 20 h. After further incubation for 4 h with MTT solution (0.25 mg/mL), the medium was aspirated, and the formazan was dissolved in DMSO. The optical density was measured at a wavelength of 570 nm (Spark 10 M, Tecan, Switzerland) and the cell viability was calculated by comparison with the control group [39]. Lipopolysaccharide (LPS from Salmonella enterica serotype typhimurium, L6511) and dexamethasone were purchased from Sigma-Aldrich, St. Louis, MO, USA.

Measurement of Nitric Oxide (NO) and Cytokine Production
The cells were plated at a density of 1 × 10 5 cells/well in 96-well plates and were then stimulated with LPS (0.5 µg/mL) in various concentrations of compound for 20 h. The cell supernatants were collected for measurements of NO and cytokines, such as TNF-α and IL-6. NO was measured by the detection of its stable oxidative metabolite, nitrite. In brief, the cell supernatant (100 µL) was mixed with the same volume of Griess reagent. After reaction for 10 min at RT, the optical density was measured at 540 nm by a microplate reader (Spark10M, Tecan Science and Technology) and the nitrite concentration was determined using a sodium nitrite standard curve. The levels of TNF-α and IL-6 were measured using enzyme-linked immunosorbent assay (ELISA) kits (BD Biosciences, Santa Clara, CA, USA) according to the manufacturer's protocols [40]. 2-Amino-4-methylpyridine (AMP) and dexamethasone (Dex) were used as positive controls.

Immunocytochemistry
Cells were adhered to 8-chamber slides, and the media was changed. After incubating with compounds 4 and 7 for 1 h and more, they were incubated with or without LPS (0.5 µg/mL). The cells were fixed with 4% formaldehyde and washed with phosphatebuffered saline (PBS). Then, the cells were permeabilized with Triton X-100 (0.2% in PBS) and incubated with primary antibody at 4 • C overnight. After washing, the membranes were incubated with a secondary antibody (Alexa Fluor 488, Thermo Fisher) for 1 h at RT. After further washing, the cells were incubated with Hoechst 33,342 to stain nuclei and measured under a fluorescence microscope (Axio Observer Z1, Carl Zeiss, Germany). The fluorescence intensity of p65 translocated to the nucleus of each cell was calculated by ImageJ software (ver. 1.53a, National Institutes of Health, Bethesda, MD, USA) and all fluorescence intensity values for each experimental group were normalized to the LPS-only treatment group.

Statistical Analyses
Data are indicated as the means ± standard deviation of the mean (S. D), and all experiments were carried out in triplicate. The statistical analysis was performed using Dunnett's test in Prism 5 software (GraphPad software, San Diego, CA, USA). A p < 0.05 was considered statistically significant and is expressed as an asterisk.

Conclusions
In the current study, phytochemicals derived from C. lineata pods, a flavonol (1), flavanonols (2 and 3), isoflavonoid derivatives (4)(5)(6)(7)(8)(9)(10)(11), and phenolic compounds (12 and 13), were obtained using bioactivity-guided isolation, and their chemical structures were identified by spectroscopic techniques, including NMR, QTOF-MS, and CD. To the best of our knowledge, the present study is the first to report that compounds 1-13 are phytochemical components of C. lineata pods. All isolates were screened for their inhibitory effect on LPS-enhanced inflammatory mediators, such as NO, IL-6, and TNF-α, in macrophages. Among compounds 1-13, isoflavonoid derivatives prunetin (4) and cajanin (7) exhibited potent anti-inflammatory activity, and cajanin (7) proved to be an effective bioactive component that compared to prunetin more greatly inhibits NF-κB activity (4). These results suggested that differences in the chemical structure of the B ring of isoflavonoids may be associated with anti-inflammatory effects. Therefore, cajanin (7), which is elicited from an unused natural resource, C. lineata pods, could be a useful candidate for the treatment of inflammatory diseases.