New Bis-Cyclometalated Iridium(III) Complexes with β-Substituted Porphyrin-Arylbipyridine as the Ancillary Ligand: Electrochemical and Photophysical Insights

An efficient synthetic access to new cationic porphyrin-bipyridine iridium(III) bis-cyclometalated complexes was developed. These porphyrins bearing arylbipyridine moieties at β-pyrrolic positions coordinated with iridium(III), and the corresponding Zn(II) porphyrin complexes were spectroscopically, electrochemically, and electronically characterized. The features displayed by the new cyclometalated porphyrin-bipyridine iridium(III) complexes, namely photoinduced electron transfer process (PET), and a remarkable efficiency to generate 1O2, allowing us to envisage new challenges and opportunities for their applications in several fields, such as photo(catalysis) and photodynamic therapies.

Although the exploitation of organo-Ir(III) based complexes in medicine is still in an early stage of development, the studies already done showed their potential as biological probes, protein inhibitors, antimicrobial, and anticancer drugs [19][20][21][22][23][24]. Compared with other transition-metal complexes, cyclometalated iridium(III) complexes with d 6 electronic structure have attractive photophysical features, namely tunable excitation and emission wavelengths, high Stokes shift, strong spin-orbit coupling of the iridium ion, high luminescence quantum yields, and relatively long phosphorescence lifetimes [1,2,[21][22][23]25]. The electronic structure have attractive photophysical features, namely tunable excitation and emission wavelengths, high Stokes shift, strong spin-orbit coupling of the iridium ion, high luminescence quantum yields, and relatively long phosphorescence lifetimes [1,2,[21][22][23]25]. The relative inertness of Ir(III) complexes, due to the stability induced by the coordinative bonds involving the metal, is also an advantage in drug design, allowing the active molecule to reach the desired target [26,27].
Our interest is centered on the functionalization of β-pyrrolic positions of mesotetraarylporphyrins aiming to prepare new porphyrinic derivatives with adequate properties to be used in different fields [57], namely as photosensitizers in Photodynamic Therapy (PDT) or antimicrobial PDT [13,[58][59][60][61][62][63][64]. We then report here an efficient synthetic approach to obtain a novel series of mononuclear heteroleptic porphyrin-iridium(III) complexes 4 and 5 via porphyrin derivatives 2 bearing a bipyridine unit (see Schemes 1 and 2). The free base and Zn(II) complexes of porphyrin-iridium(III) derivatives were electrochemically, spectroscopically and electronically characterized, and their features were compared with the corresponding porphyrin-arylbipyridine derivatives 2. The Zn(II) porphyrin-iridium(III) complexes showed thermodynamically favorable photoinduced electron transfer process (PET) and a remarkable ability to generate singlet oxygen (ФΔ). Scheme 2. Synthetic route leading to porphyrinic iridium(III) complexes 4 and 5.

Synthesis and Structural Characterization
In a previous work we reported an efficient access to a porphyrin-chalcone type derivative 1 from reaction of 2-formyl-tetraphenylporphyrin (TPP-CHO) with 2acetylpyridine (see Supplementary Information, Scheme S1) [65]. Additionally, we had verified that, in the presence of ammonium acetate and catalytic amounts of lanthanum triflate, the chalcone moieties reacted further, affording terpyridine units via Kröhnke type reaction [66].
These facts prompted us to envisage an easy synthetic approach to porphyrinic ligands of type 2 bearing a bipyridine unit (Scheme 1) that could be further used to replace the 2,20-bipyridine (bpy) in the archetypal [(ppy)2Ir(bpy)][PF6] complex.
In a typical experiment, a toluene solution of porphyrin 1, acetophenone (5.0 equiv.), ammonium acetate (6.0 equiv.), and La(OTf)3 (20 mol%), was heated at reflux for 3 h. A TLC of the reaction mixture revealed the total consumption of the starting porphyrin and the formation of two new products. After the workup, the two new compounds were separated by column chromatography (silica gel). The minor and less polar compound isolated was identified by NMR and mass spectrometry as being the benzoporphyrin derivative 3a (24%); the major compound isolated in 72% yield was identified as the desired porphyrin-bipyridine derivative 2a. The formation of the porphyrin-phenyl pyridine derivatives follows the Kröhnke-type condensation, while the benzoporphyrinic side-products were obtained via an 1,6-Michael addition followed by intramolecular cyclization (see Scheme S2 at Supplementary Information). Briefly, a diketone intermediate is obtained by 1,6-Michael addition reaction of a carbanion to the beta-Scheme 2. Synthetic route leading to porphyrinic iridium(III) complexes 4 and 5.

Synthesis and Structural Characterization
In a previous work we reported an efficient access to a porphyrin-chalcone type derivative 1 from reaction of 2-formyl-tetraphenylporphyrin (TPP-CHO) with 2-acetylpyridine (see Supplementary Information, Scheme S1) [65]. Additionally, we had verified that, in the presence of ammonium acetate and catalytic amounts of lanthanum triflate, the chalcone moieties reacted further, affording terpyridine units via Kröhnke type reaction [66].
These facts prompted us to envisage an easy synthetic approach to porphyrinic ligands of type 2 bearing a bipyridine unit (Scheme 1) that could be further used to replace the 2,20-bipyridine (bpy) in the archetypal [(ppy) 2 Ir(bpy)][PF 6 ] complex.
In a typical experiment, a toluene solution of porphyrin 1, acetophenone (5.0 equiv.), ammonium acetate (6.0 equiv.), and La(OTf) 3 (20 mol%), was heated at reflux for 3 h. A TLC of the reaction mixture revealed the total consumption of the starting porphyrin and the formation of two new products. After the workup, the two new compounds were separated by column chromatography (silica gel). The minor and less polar compound isolated was identified by NMR and mass spectrometry as being the benzoporphyrin derivative 3a (24%); the major compound isolated in 72% yield was identified as the desired porphyrin-bipyridine derivative 2a. The formation of the porphyrin-phenyl pyridine derivatives follows the Kröhnke-type condensation, while the benzoporphyrinic sideproducts were obtained via an 1,6-Michael addition followed by intramolecular cyclization (see Scheme S2 at Supplementary Information). Briefly, a diketone intermediate is obtained by 1,6-Michael addition reaction of a carbanion to the beta-pyrrolic 3-position nearest the chalcone-type unit of derivative 1, followed by an intramolecular aldol-type condensation and aromatization with benzaldehyde elimination.
The structures of derivatives 2 were unambiguously established by spectroscopic data, namely NMR spectroscopy ( 1 H, 13 C, and COSY) and mass spectrometry techniques (Figures S1-S14 in Supplementary Information). The asymmetry of the bipyridine moiety was confirmed by the 1 H NMR spectra with two distinguishing duplets at ca. δ 7.5 and δ 8.5 ppm with a coupling constant of 1.2 Hz due to the resonance of the protons 3 and 5 from the tri-substituted pyridine. The 1 H NMR spectra also show, in the low field region, signals corresponding to the resonance of β-pyrrolic protons and of protons 3 and 6 from the mono-substituted pyridine moiety; the resonances corresponding to the two remaining protons from this moiety appear for proton 4 together with the resonances due to ortho protons from meso-phenyl groups as a multiplet at ca. δ 8.0-7.8 ppm and for proton 5 as a multiplet at ca. δ 7.4-7.3 ppm.
The desired iridium(III) complexes 4a-c were obtained by heating a suspension of porphyrin-bipyridine derivatives 2a-c in methanol with [Ir 2 (ppy) 4 Cl 2 ] (1.1 equiv.) in a sealed tube at 120 • C (Scheme 2). The monitorization of the reaction progress by TLC showed, after 2 h, the total consumption of the starting porphyrin-bipyridine derivatives 2, with the formation of a more polar product. After the work-up, chromatographic purification, and crystallization (see details in Materials and Methods), compounds 4a-c were isolated in excellent yields (93-96%) and their structures were confirmed by NMR spectroscopy ( 1 H, 13 C, and COSY) and mass spectrometry techniques (Figures S16-S39 in Supplementary Information).
Although the introduction of the [Ir(ppy) 2 ] moiety induced a higher complexity in the NMR spectra, a characteristic pattern in the aromatic region, from ca. δ 9.2 to δ 5.4 ppm, assigned to the highly asymmetrical phenylpyridine moieties can be visualized (see Supplementary  Complexes 5a-c were obtained in quantitative yields by metalation of the respective free base derivatives 4a-c with zinc(II) acetate according to conventional procedures [67]. The 1 H NMR of compounds 5a-c showed the disappearance of the resonances of the inner NH at high fields but maintain the same profile observed for the proton resonances of the porphyrin-bipyridine groups and [Ir(ppy)] + moieties (Supplementary Information, Figures S40-S51).

Electrochemistry
The cyclic voltammograms of the synthesized porphyrins were performed using DMF as solvent ( Figure S52). As an example, Figure 1 shows a typical cyclic voltammetry of TPP and of compounds 2c and 4c. The studied systems show multiple redox processes, and the peak potentials are dependent on the nature of the macrocycle substituents as well as of the presence of the metal ion in the macrocycle core (Table 1). It is known that the redox potentials are influenced by the π-extension of the aromatic ring [41].  Two reversible reduction processes are observed in TPP, at E 1/2 = −1.41 VFc+/Fc and at E 1/2 = −1.86 VFc+/Fc, corresponding to the reduction of the porphyrinic π-system [41]. An oxidation process at 0.76 VFc+/Fc was also observed and was attributed to the oxidation of the porphyrin ring, leading to the formation of a π-radical cation [41]. In the TPP Zn(II) derivative (ZnTPP), the oxidation with the formation of a porphyrin π-radical cation occurs at lower potential, 0.51 VFc+/Fc. A second one-electron oxidation process occurs on the porphyrin ring at higher potential. The coordination of Zn 2+ on the TPP site shifts the reduction peaks to more negative values, respectively, −1.69 and −2.07 VFc+/Fc. The change in the electrochemical potential of porphyrins with the Zn(II) metalation is consistent with the UV-visible spectrum change. The insertion of electroactive moieties on the porphyrins generates more complex cyclic voltammograms with an increased number of peaks. As an example, the cyclic voltammogram of 2c shows an extra reduction at −2.07 VFc+/Fc that can be attributed to the process centered on the bipyridine ligand.
For the binuclear species 4 and 5, the changes in the oxidation and reduction peaks are consistent with the electroactivity of the introduced iridium(III) moiety (Table 1 presents the potentials of the first oxidation and first reduction peaks). The introduction of the iridium(III) counterpart in the β-pyrrolic position causes noticeable changes in the redox processes, which are localized on porphyrin centers (TPP and ZnTPP nucleus for compounds 4a-c and 5a-c, respectively). The first oxidation potentials of porphyrin  Two reversible reduction processes are observed in TPP, at E 1/2 = −1.41 V Fc+/Fc and at E 1/2 = −1.86 V Fc+/Fc , corresponding to the reduction of the porphyrinic π-system [41]. An oxidation process at 0.76 V Fc+/Fc was also observed and was attributed to the oxidation of the porphyrin ring, leading to the formation of a π-radical cation [41]. In the TPP Zn(II) derivative (ZnTPP), the oxidation with the formation of a porphyrin π-radical cation occurs at lower potential, 0.51 V Fc+/Fc . A second one-electron oxidation process occurs on the porphyrin ring at higher potential. The coordination of Zn 2+ on the TPP site shifts the reduction peaks to more negative values, respectively, −1.69 and −2.07 V Fc+/Fc . The change in the electrochemical potential of porphyrins with the Zn(II) metalation is consistent with the UV-visible spectrum change. The insertion of electroactive moieties on the porphyrins generates more complex cyclic voltammograms with an increased number of peaks. As an example, the cyclic voltammogram of 2c shows an extra reduction at −2.07 V Fc+/Fc that can be attributed to the process centered on the bipyridine ligand.
For the binuclear species 4 and 5, the changes in the oxidation and reduction peaks are consistent with the electroactivity of the introduced iridium(III) moiety ( Table 1 presents the potentials of the first oxidation and first reduction peaks). The introduction of the iridium(III) counterpart in the β-pyrrolic position causes noticeable changes in the redox processes, which are localized on porphyrin centers (TPP and ZnTPP nucleus for compounds 4a-c and 5a-c, respectively). The first oxidation potentials of porphyrin nucleus in 4a-c are similar to that found for TPP (0.72 V Fc+/Fc (4a), 0.74 V Fc+/Fc (4b), 0.76 V Fc+/Fc (4c), and 0.76 V Fc+/Fc (TPP)), while their first reduction potentials are less negative (−1.26 V Fc+/Fc (4a), −1.27 V Fc+/Fc (4b), and −1.28 V Fc+/Fc (4c), vs −1.41 V Fc+/Fc (TPP)). The same trend is observed in the CV of the zinc(II) complexes (first reduction potentials of −1.34 V Fc+/Fc (5a), −1.11 V Fc+/Fc (b) and −1.10 V Fc+/Fc (5c) compared to −1.69 V Fc+/Fc (ZnTPP)). This means that the porphyrinic core is more easily reduced in the porphyrin-iridium(III) dyads. The energy gap is also narrower in the new synthesized porphyrins.

Spectroscopic and Photophysical Properties
The absorption spectra of free base porphyrins 2a-c, porphyrin-iridium(III) complexes 4a-c and zinc(II) porphyrin-iridium(III) complexes 5a-c were recorded in DMF at room temperature. A comparison of the UV-Vis spectra of porphyrin-iridium(III) complexes 4b and 5b, along with their parent porphyrin-bipyridine derivatives 2b, is shown in Figure 2A (Table S1 for all compounds). These spectra were chosen as representatives of each type of compounds prepared. The absorption spectra of derivatives 2a-c in DMF (room temperature) show the typical features of free base porphyrins-the highly intense Soret band at ca. 423 nm (due to the allowed π-π* transitions from S 0 -S 2 ) and the four weak Q bands (from S 0 -S 1 transitions). As expected, the UV-Vis spectra of porphyrin-iridium(III) complexes 4a-c confirm porphyrin functionalization with an external [Ir(ppy) 3 ] metal center as revealed by the presence of the typical bands of both allies; the intense band in the UV region 250-320 nm is assigned to the singlet-singlet ligand-centered 1 LC band and the weaker absorption band 320-400 nm to metal-to-ligand charge transfer transitions (MLCT) in accordance with the well stablished absorption spectra of (Ir(CˆN)2(NˆN)) type complexes [42]. In addition, a Soret band with a maximum at 423 nm and four weaker Q bands account for the presence of porphyrin moieties in 4a-c. The absorption profile of Zn(II)-porphyrin-iridium(III) complexes 5a-c is very similar to that of 4a-c. Nonetheless, as a result of symmetry changes owing to Zn(II) coordination on porphyrin inner core, red shift Soret (431 nm) and two Q bands were found as porphyrin moiety fingerprint [68]. The absorption spectra of porphyrins 4a-c and 5a-c are almost identical to the sum of the absorption spectra of the two separated moieties, supporting the idea that ground state inter-or intramolecular interaction between porphyrin and Ir(III) centers are absent under these experimental conditions, in line with other porphyrin-iridium(III) external complexes linked via axial coordination of the iridium complex pyridine to the porphyrinic zinc center [69].
Upon photoexcitation, derivatives 2a-c, 4a-c, and 5a-c yield important NIR emission ranging from 600 to 800 nm ( Figure 2B). In order to investigate the emission profiles of the new systems and to check for the possibility of iridium/porphyrin communication through photoinduced energy/electron transfer, derivatives 4a-c and 5a-c were studied by steady state spectroscopy upon excitation into the iridium(III) 1 CT band (λ exc = 360 nm) and also into porphyrin Q bands (λ exc = 565 nm). The same measurements were performed for parent compounds 2a-c ( Figure 2B,C). The emission spectra of porphyrin-Ir(III) complexes 4a-c obtained after excitation at ca. 565 nm present two bands centered at ca. 675 and ca. 727 nm ( Figure 2B and Figure S53 in Supplementary Information). Incorporation of Zn(II) in the porphyrin core induced the expected blue shift in the emission bands of 5a-c to 641 and 667 nm. Interestingly, upon excitation at 360 nm, where the absorption spectra of the porphyrin-iridium(III) complex is mainly due to the iridium moiety, the emission spectra of 4a-c show a fairly strong emission at approximately 671 and 724 nm, which is characteristic of porphyrin units. A close inspection of the superposition of the fluorescence excitation spectra of compounds 4a-c and 5a-c with their correspondent absorption spectra (measured in diluted DMF solutions and normalized to the absorption spectra in the Q bands region where the iridium moiety does not absorb) reveals that it lacks Ir(ppy) 3 typical bands ( Figure 2D for 4a). It might be worth noting that excitation spectra of 4a is very similar to excitation spectra measured for porphyrin-bipyridine parent 2a ( Figure 2D), foreseeing that the absorption of light from the Ir(III) β-substituent does not contribute significantly for the observed fluorescence. Based on our experimental data, there is no clear evidence of Ir(III) to porphyrin singlet-singlet energy transfer. Nonetheless, fluorescence measured for 4a-c and 5a-c from excitation at 360 nm should be mainly due to the residual absorption of the porphyrin moiety and is also observed for reference TPP (see Figure S54 in Supplementary Information for details). does not contribute significantly for the observed fluorescence. Based on our experimental data, there is no clear evidence of Ir(III) to porphyrin singlet-singlet energy transfer. Nonetheless, fluorescence measured for 4a-c and 5a-c from excitation at 360 nm should be mainly due to the residual absorption of the porphyrin moiety and is also observed for reference TPP (see Figure S54 in Supplementary Information for details). Fluorescence recorded for all compounds 2a-c, 4a-c, and 5a-c (non-degassed DMF solutions) shows that porphyrin-iridium(III) and Zn(II)porphyrin-iridium(III) fluorescence is significantly quenched with respect to reference TPP. Fluorescence quantum yields were estimated by a comparative method with a reference compound (TPP in DMF, [ΦFlu] = 0.11) [70] and are reported in Table 2. Fluorescence from S1 is reduced to 45% for the porphyrin-iridium dyads 4a-c and to only 27% for the corresponding zinc complexes 5a,b (18% for 5c) by comparison with model porphyrin TPP (See Table 1). Fluorescence lifetime measurements also confirm that porphyrins fluorescence is efficiently quenched by the β appended [Ir(ppy)2] moiety ( Table 1).
The introduction of bipyridine and iridium(III) complexes as β-pyrrolic substituents increases the complexity of fluorescence lifetime decays. While a monoexponential function can be successfully used to fit experimental TPP and ZnTPP decays (11.0 ns and 2.0 ns, in accordance with literature data), monoexponential decays were never observed for  Supplementary Information (Figure S55).
Fluorescence recorded for all compounds 2a-c, 4a-c, and 5a-c (non-degassed DMF solutions) shows that porphyrin-iridium(III) and Zn(II)porphyrin-iridium(III) fluorescence is significantly quenched with respect to reference TPP. Fluorescence quantum yields were estimated by a comparative method with a reference compound (TPP in DMF, [Φ Flu ] = 0.11) [70] and are reported in Table 2. Fluorescence from S1 is reduced to 45% for the porphyrin-iridium dyads 4a-c and to only 27% for the corresponding zinc complexes 5a,b (18% for 5c) by comparison with model porphyrin TPP (See Table 1). Fluorescence lifetime measurements also confirm that porphyrins fluorescence is efficiently quenched by the β appended [Ir(ppy) 2 ] moiety (Table 1). Table 2. Fluorescence lifetimes (τ i ), respective pre-exponential factors (A i ), and fluorescence quantum yields (Φ F ) measured for porphyrins 2, 4, and 5 a-c in DMF (air equilibrated samples). The introduction of bipyridine and iridium(III) complexes as β-pyrrolic substituents increases the complexity of fluorescence lifetime decays. While a monoexponential function can be successfully used to fit experimental TPP and ZnTPP decays (11.0 ns and 2.0 ns, in accordance with literature data), monoexponential decays were never observed for 2a-c, 4a-c, and 5a-c derivatives. In fact, all the decays were best fitted with a sum of two exponentials (Table 1) The interpretation of these complex decays is not straightforward. With basis on NMR data, the existence of a mixture of β-pyrrolic substituted/non-substituted porphyrins, concerning bipyridine and iridium(III) complexes counterparts, was refuted. A probable explanation for the observed multiexponential decay may arise from the possibility of these porphyrins to exist in solution as a mixture of tautomers, [71] different conformers, or may likely reflect the existence of Ir(III) structural isomers (∆ and Λ).
For compounds 5a-c, the shorter-lived component (0.6-0.8 ns) now has a higher contribution (88-93%). A residual component with fluorescence lifetime resembling the one of ZnTPP was observed (2.0 ns). Time evolution of the fluorescence investigated by means of direct absorption spectroscopy (DAS) spectroscopy can be found in Supplemtary  Information (Figure S56).
In DMF degassed solutions, the emission spectra of porphyrin-iridium(III) complexes 4a-c and 5a-c shows an additional broad and unresolved blue shifted band with a maximum between 498-506 nm, which is absent in the emission spectra of iridium free compounds 2a-c. This band disappears in non-degassed solutions, as stated in Figure S57. Additionally, the solution emission lifetimes are in the microsecond range (ca. τ (5a) = 2.6 µs, Figure S58 and Table S2), which are substantially higher than those obtained in nondegassed solutions. Emission data are in accordance with the ones reported previously for Ir(ppy) 3 complexes in degassed toluene and acetonitrile and are attributed to the phosphorescence of the Ir(III) peripheral subunit [72,73]. The porphyrin typical fluorescence bands observed between 600-800 nm are unchanged.
To understand the feasibility of photoinduced electron transfer process (PET), the free energy of this process (∆G PET ) was estimated using the following equation [74] ∆G PET = E CT (D-A) -E 0-0 (D) (1) where E CT (D-A) is the energy of the charge transfer state of donor and acceptor obtained from the first oxidation and first reduction potentials determined by cyclic voltammetry (E gap in Table 1) and E 0-0 (D) is the zero-to-zero transition of porphyrin donor determined by the intersection of the normalized absorption and emission spectra. The results are summarized in Table 3.  (1) obtained from the optical gap, (2) determined by Equation (1).
The calculated values predict that PET is thermodynamically favorable for Zn(II) complexes 5a-c. Other photoinduced processes might be in the origin of porphyrin fluorescence quenching observed: (i) an increase in the porphyrin radiative rate constant; (ii) porphyrin enhanced non radiative return to the ground state; (iii) porphyrin improved intersystem crossing to the triplet state.
Singlet oxygen luminescence measurements at 1270 nm were performed for all compounds in order to determine Φ ∆ by a reference methodology, which uses TPP as standard in DMF (Φ ∆ = 0.65) [76,77]. The optical density of all the solutions was adjusted to 0.1 at 420 nm. As an example, the phosphorescence spectrum and a decay profile for complex 4b is shown in Figure 3. The experimental results obtained for all compounds are reported in Table 4.
The experimental data clearly demonstrate that porphyrin-bipyridine iridium(III) 4a-c and 5a-c complexes are better 1 O 2 generators than the respective precursors 2a-c (and also than reference TPP). For porphyrins 4a-c, singlet oxygen quantum yields were found to be 9-14% higher than those determined for porphyrin free-bases 2a-c. It is well known from the literature that the presence of a metal ion in the porphyrinic inner core of a porphyrin increases the spin orbital coupling and the intersystem, crossing to the triplet state in a classical phenomenon known as heavy atom effect [78]. Our results indicate that the presence of an external heavy iridium(III) moiety also shows a similar effect to that extent. An additional increase of 12-21% singlet oxygen quantum yield was found for Zn(II) porphyrins 5a-c when compared to their precursors 4a-c. This high ability to photogenerate 1 O 2 allows us to consider this series of compounds as potential candidates for light-driven therapy. Considering such an important increase in singlet oxygen quantum yield, porphyrin fluorescence quenching observed for porphyrin-bipyridine iridium(III) complexes 4a-c and for the corresponding zinc(II) complexes 5a-c is attributed to the presence of external and/or internal metal Ir/Zn centers, respectively. intersystem crossing to the triplet state.
Singlet oxygen luminescence measurements at 1270 nm were performed for all compounds in order to determine ΦΔ by a reference methodology, which uses TPP as standard in DMF (ΦΔ = 0.65) [76,77]. The optical density of all the solutions was adjusted to 0.1 at 420 nm. As an example, the phosphorescence spectrum and a decay profile for complex 4b is shown in Figure 3. The experimental results obtained for all compounds are reported in Table 4. The experimental data clearly demonstrate that porphyrin-bipyridine iridium(III) 4a-c and 5a-c complexes are better 1 O2 generators than the respective precursors 2a-c (and also than reference TPP). For porphyrins 4a-c, singlet oxygen quantum yields were found to be 9-14% higher than those determined for porphyrin free-bases 2a-c. It is well known from the literature that the presence of a metal ion in the porphyrinic inner core of a

Materials and Methods
3.1. General Remarks 1 H and 13 C solution NMR spectra were recorded on Bruker Avance 300 (300.13 and 75.47 MHz, respectively), 500 (500.13 and 125.76 MHz, respectively), and 700 (700.13 MHz) spectrometers. CDCl 3 was used as solvent and tetramethylsilane (TMS) as internal reference; the chemical shifts are expressed in δ (ppm) and the coupling constants (J) in Hertz (Hz).
Unequivocal 1 H assignments were made using 2D COSY ( 1 H/ 1 H), while 13 C assignments were made based on 2D HSQC ( 1 H/ 13 C) and HMBC (delay for long-range J C/H couplings were optimized for 7 Hz) experiments. Mass spectra were recorded using MALDI TOF/TOF 4800 Analyzer, Applied Biosystems MDS Sciex, with CHCl 3 as solvent and without matrix. Electrospray ionization mass spectra were acquired with a Micromass Q-Tof 2 (Micromass, Manchester, UK), operating in the positive ion mode, equipped with a Z-spray source, an electrospray probe, and a syringe pump. Source and desolvation temperatures were 80 • C and 150 • C, respectively. Capillary voltage was 3000 V. The spectra were acquired at a nominal resolution of 9000 and at cone voltages of 30 V. Nebulization and collision gases were N 2 and Ar, respectively. Porphyrin solutions in methanol were introduced at a 10 µL/min flow rate. Mass spectra HRMS-ESI(+) were recorded on a LTQ Orbitrap XL mass spectrometer (Thermo Fischer Scientific, Bremen, Germany) using CHCl 3 as solvent. The UV-Vis spectra were recorded on an UV-2501PC Shimadzu spectrophotometer using DMF as solvent. Preparative thin-layer chromatography was carried out on 20 × 20 cm glass plates coated with silica gel (0.5 mm thick). Column chromatography was carried out using silica gel (35-70 mesh, Merck, Darmstadt, Germany). Analytical TLC was carried out on precoated sheets with silica gel (Merck 60, 0.2 mm thick).
All the chemicals were used as supplied. Solvents were purified or dried according to the literature procedures [79].

Synthesis of the 2-[3-oxo-3-(pyridin-2-yl)prop-1-en-1-yl]-5,10,15,20tetraphenylporphyrin, 1
To a solution of 2-acetylpyridine (1.2 equiv.) in dry toluene (1 mL), piperidine (1.5 equiv.) was added, and the mixture was stirred for 30 min at room temperature. After this time, 2-formyl-5,10,15,20-tetraphenylporphyrin (TPP-CHO) and La(OTf) 3 (20 mol%) were added and the resulting mixture was heated at reflux for 24 h. After cooling, the reaction mixture was washed with water and extracted with chloroform. The organic layer was separated, dried under Na 2 SO 4 , and the solvent was evaporated under reduced pressure. The crude mixture was purified by column chromatography (silica gel) using toluene-light petroleum (1:1) and toluene as the eluent. The compound isolated was then crystallized from CH 2 Cl 2 -hexane and fully characterized by NMR, mass and UV-Vis techniques. The structure of compound 1 is in accordance with the literature [65].

Synthesis of the Porphyrin-Bipyridine Derivatives 2a-c: General Procedure
To a solution of the appropriate acetophenone (5.0 equiv.) in dry toluene (1 mL), ammonium acetate was added (6.0 equiv.), and the mixture was stirred for 30 min at room temperature. After this time, porphyrin-chalcone type derivative 1 and La(OTf) 3 (20 mol%) were added to the mixture and heated at reflux for 3 h. After cooling, the reaction mixture was washed with water and extracted with dichloromethane. The organic phase was dried (Na 2 SO 4 ) and the solvent was evaporated under reduced pressure. The crude mixture was submitted to column chromatography (silica gel) using CH 2 Cl 2 as eluent. The fractions obtained were fully characterized by NMR, mass, UV-Vis, and fluorescence techniques. The reactional conditions and yields are summarized in Scheme 1.

Synthesis of Compounds 4a-c: General Procedure
In a sealed tube, the dichloro-bridged diiridium complex [Ir(ppy) 4 Cl] 2 (1.1 equiv.) was added to the appropriate porphyrin-bipyridine 2a-c in methanol (1 mL). Then, the resulting suspension was heated at 120 • C for 2 h. After cooling, an excess of an aqueous solution of KPF 6 was added to the mixture and the resulting precipitate was filtered, washed several times with water, and then with diethyl ether. After being dried under vacuum, the crude mixture was submitted to column chromatography (silica gel) using CH 2 Cl 2 /MeOH (97:3) as eluent. Compounds 4 were obtained after crystallization from CH 2 Cl 2 /MeOH in 93% (4a and 4b) and 96% (4c) (see Scheme 2). The compounds obtained were fully characterized by NMR, mass, UV-Vis, and fluorescence techniques.        (1.5 equiv.) for 10 min at 50 • C. The reaction was followed by TLC and UV-Vis until total consumption of the starting porphyrin. After cooling, the reaction mixture was washed with water and extracted with dichloromethane. The organic phase was dried (Na 2 SO 4 ), and the solvent was evaporated under reduced pressure. The resulting residues were crystallized from CH 2 Cl 2 /hexane, and the desired compounds 5a-c were obtained in almost quantitative yields (see Scheme 2).

Cyclic Voltammetry Measurements
Cyclic voltammetry was performed using an AUTOLAB PGSTAT 30 potentiostat with a three-electrode cell consisting of a Pt counter electrode, an Ag/AgCl reference electrode, and a glassy carbon working electrode (3 mm diameter). Solutions with 1.0 mM porphyrins were prepared in DMF with 0.1 M Bu 4 NPF 6 (TBAP) as supporting electrolyte. The voltammograms were obtained with a 0.1 V s −1 scan rate. Ferrocene in DMF (+0.39 V versus Ag/AgCl) was employed as internal standard.

Spectrophotometric and Spectrofluorimetric Measurements
Absorption spectra were recorded on a PerkinElmer Lambda spectrophotometer. Corrected fluorescence measurements were recorded in a SPEX Fluorolog spectrophotometer (Horiba Jobin Yvon). Excitation at 445 nm was achieved using a NanoLED (fwhm < 1.0 ns) at a repetition rate of 1 MHz. The spectrophotometric characterizations were performed by preparing a stock solution of the compound in DMF (ca. 10 −4 M) in a 5 mL volumetric flask. The studied solutions were prepared by appropriate dilution of the stock solution to 6 × 10 −6 M. Luminescence quantum yields of the studied compounds 2a-c, 4a-c, and 5a-c were measured in 1 × 1 cm quartz optical cells on a F4500-Hitachi spectrofluorimeter using a solution of 5, 10, 15, 20-tetraphenylporphyrin (TPP) in DMF as standard ([Φ Flu ] = 0.11) [70,82,83]. All the measurements were performed at 298 K.

Singlet Oxygen Generation
Singlet oxygen measurements were performed in a specially designed Edinburgh F900 instrument (Edinburgh, UK) consisting of a Rainbow OPO (Quantel Laser-France) 10 Hz, 2 mJ/pulse, which was pumped by a Brilliant NdYAG laser (Quantel Laser-France) and equipped with a cuvette holder, a silicon filter, monochoromator, a liquid nitrogencooled NIR PMT (R5509) (Hamamatsu Co., Bridgewater, NJ, USA), and a fast multiscaler analyzer card with 5 ns/channel (MSA-300; Becker and Hickl, Berlin, Germany). All samples have the same OD (about 0.1) at the excitation wavelength, as revealed by an UV-Vis Spectrometer (Shimadzu 2400, Kioto, Japan). A volume of 3 mL of each solution was put into quartz cells (1 cm × 1 cm) and stored in the dark. The samples were irradiated at 420 nm inside a fluorescence quartz cuvette. Photosensitized steady-state singlet oxygen luminescence was measured at 1270 nm. The intensities of emission peak at 1270 nm are correlated with the amount of 1 O 2 generated. Kinetic curves of the 1 O 2 luminescence were measured using the method of time-correlated single photon counting. To calculate the singlet oxygen quantum yield (ϕ ∆ ), TPP in DMF was used as reference (ϕ ∆ = 0.65) [76].

Conclusions
In summary, this study allowed us to develop a straightforward synthetic pathway to prepare, for the first time, a new series of porphyrins bearing arylbipyridine units coordinated with iridium(III) at a β-pyrrolic position. The free base porphyrin-iridium(III) derivatives, as well as the corresponding Zn(II) complexes, were successfully synthesized with excellent yields (≥93%).
The photophysical/photochemical characterization showed that the introduction of the cyclometaled [Ir(ppy) 2 ] units at the porphyrin-arylbipyridine led to conjugates with superior and outstanding ability to generate singlet oxygen compared to TPP. The overall structural, electrochemical, and photophysical properties of 4 and 5 are encouraging and further studies will be performed to investigate these complexes in photoinduced and photodynamic processes, namely as photocatalysts and as photosensitizers agents.