Harnessing the Role of Foliar Applied Salicylic Acid in Decreasing Chlorophyll Content to Reassess Photosystem II Photoprotection in Crop Plants

Salicylic acid (SA), an essential plant hormone, has received much attention due to its role in modulating the adverse effects of biotic and abiotic stresses, acting as an antioxidant and plant growth regulator. However, its role in photosynthesis under non stress conditions is controversial. By chlorophyll fluorescence imaging analysis, we evaluated the consequences of foliar applied 1 mM SA on photosystem II (PSII) efficiency of tomato (Solanum lycopersicum L.) plants and estimated the reactive oxygen species (ROS) generation. Tomato leaves sprayed with 1 mM SA displayed lower chlorophyll content, but the absorbed light energy was preferentially converted into photochemical energy rather than dissipated as thermal energy by non-photochemical quenching (NPQ), indicating photoprotective effects provided by the foliar applied SA. This decreased NPQ, after 72 h treatment by 1 mM SA, resulted in an increased electron transport rate (ETR). The molecular mechanism by which the absorbed light energy was more efficiently directed to photochemistry in the SA treated leaves was the increased fraction of the open PSII reaction centers (qp), and the increased efficiency of open reaction centers (Fv’/Fm’). SA induced a decrease in chlorophyll content, resulting in a decrease in non-regulated energy dissipated in PSII (ΦNO) under high light (HL) treatment, suggesting a lower amount of triplet excited state chlorophyll (3Chl*) molecules available to produce singlet oxygen (1O2). Yet, the increased efficiency, compared to the control, of the oxygen evolving complex (OEC) on the donor side of PSII, associated with lower formation of hydrogen peroxide (H2O2), also contributed to less creation of ROS. We conclude that under non stress conditions, foliar applied SA decreased chlorophyll content and suppressed phototoxicity, offering PSII photoprotection; thus, it can be regarded as a mechanism that reduces photoinhibition and photodamage, improving PSII efficiency in crop plants.


Introduction
The world's crop productivity is decaying while population growth is rising, placing increasing demands on agriculture [1]. The challenge of optimizing crop performance by increasing the efficiency and productivity of photosynthesis in crop plants is an essential Measurements of chlorophyll a fluorescence have been extensively used to probe the functions of photosynthetic machinery, especially of PSII [15,[26][27][28]. Chlorophyll a fluorescence measurements can provide information about the direct or indirect effects of SA on the light energy utilization in PSII [24]. The light energy absorbed by PSII antennae can be either utilized via photochemistry or competitively dissipated via various thermal processes [29][30][31][32]. The conversion efficiency of absorbed light energy to photochemical energy is critical in describing plant productivity over time [33]. The light reactions of photosynthesis involve a set of redox reactions that are the source of reducing power and energy for producing organic compounds [34]. When the light energy absorbed by photosystem II (PSII) and photosystem I (PSI) exceeds the amount that can be used for photochemistry, the consequence is the increased formation of reactive oxygen species (ROS), such as singlet oxygen ( 1 O 2 ), superoxide anion radical (O 2 • −), and hydrogen peroxide (H 2 O 2 ) [35][36][37][38][39]. Under excess light conditions, the overexcitation of PSII increases the probability of the formation of the triplet chlorophyll state ( 3 Chl*) from the singlet excited states ( 1 Chl*) through the intersystem crossing, producing single oxygen ( 1 O 2 ) [39][40][41][42][43]. Electron leakage to O 2 at PSI results in the superoxide anion radical (O 2 • −) that via a disproportionation reaction is catalyzed by superoxide dismutase (SOD) and is converted to hydrogen peroxide (H 2 O 2 ) [44,45], which is the most stable and least reactive ROS with the longest lifetime, as it is able to easily diffuse through the membranes [46]. To prevent the formation of ROS and photoinhibition, the absorbed light energy by the light-harvesting complexes must match the rate of electron transport from PSII to PSI [47,48].
In the present study, we evaluated under optimal growth conditions of tomato plants the consequences of foliar applied 1 mM SA for 72 h on photosystem II (PSII) function and estimated the effects of SA application on reactive oxygen species (ROS) generation and chlorophyll content. Our hypothesis was that foliar application of SA will increase the quantum yield of photosystem II (PSII) (Φ PSII ) via decreasing the photoprotective energy loss (NPQ). Thus, through improving light energy distribution, SA will optimize tomato crop performance by increasing the efficiency and productivity of photosynthesis. Consequently, foliar application of SA can be regarded as a mechanism that reduces photoinhibition and photodamage, improving PSII efficiency in crop plants.

Chlorophyll Content and Maximum Efficiency of Photosystem II of Control and Salycilic Acid Treated Tomato Leaves
In each independent experiment of foliar sprayed tomato leaves by either distilled water (control) or 1 mM SA, chlorophyll contents were assessed after 72 h of treatment ( Figure 1a). The chlorophyll content, expressed in relative units, decreased significantly after 72 h of treatment with 1 mM SA (Figure 1a). In contrast to this, the maximum efficiency of PSII photochemistry (Fv/Fm) of tomato leaves increased compared to the control after 72 h of SA treatment (Figure 1b).

Light Energy Utilization in Photosystem II of Control and Salycilic Acid Treated Tomato Leaves
The changes in the light energy utilization in PSII of foliar sprayed tomato leaves, by either distilled water (control) or 1 mM salicylic acid (SA), were estimated by measuring the amount of light energy allocated for photochemistry in PSII (ΦPSII) for regulated non-photochemical energy loss; that is, for photoprotective heat dissipation (ΦNPQ) and for non-regulated energy loss in PSII (ΦNO), the sum of them to be equal to 1 [49].
The effective quantum yield of PSII photochemistry (Φ PSII ), after 72 h of treatment by 1 mM SA, increased by 11% at low light (LL) and 7.5% at high light (HL), compared to the controls (Figure 1c). At the same time, the quantum yield of regulated non-photochemical energy loss (Φ NPQ ) decreased by 1 mM SA treatment at LL without any change at HL (Figure 1d), while the quantum yield of non-regulated energy (Φ NO ) did not change by SA treatment at LL and decreased at HL compared to their controls (Figure 1e).   energy loss (ΦNPQ) decreased by 1 mM SA treatment at LL without any change at HL (Figure 1d), while the quantum yield of non-regulated energy (ΦNO) did not change by SA treatment at LL and decreased at HL compared to their controls (Figure 1e).

Changes in the Electron Transport Rate and the Excitation Pressure after Salycilic Acid Treatment
The electron transport rate (ETR) increased by 1 mM SA treatment at both LL and HL (Figure 2c), with a parallel decrease in excitation pressure at PSII (1 − qp) at both actinic light (AL) illuminations ( Figure 2d).

Changes in the Excess Excitation Energy in Photosystem II and the Efficiency of the Oxygen Evolving Complex after Salycilic Acid Treatment
The excess excitation energy (EXC), which is calculated as (Fv/Fm − Φ PSII )/Fv/Fm, decreased after 72 h of treatment by 1 mM SA at both actinic light (AL) illuminations ( Figure 3a). The efficiency of the oxygen evolving complex on the donor side of PSII (Fv/Fo) in tomato leaves after 72 h of treatment by 1 mM SA increased compared to the control plants (sprayed by distilled water) (Figure 3b).
The electron transport rate (ETR) increased by 1 mM SA treatment at both LL and HL (Figure 2c), with a parallel decrease in excitation pressure at PSII (1 − qp) at both actinic light (AL) illuminations ( Figure 2d).

Changes in the Excess Excitation Energy in Photosystem II and the Efficiency of the Oxygen Evolving Complex after Salycilic Acid Treatment
The excess excitation energy (EXC), which is calculated as (Fv/Fm − ΦPSII)/Fv/Fm, decreased after 72 h of treatment by 1 mM SA at both actinic light (AL) illuminations ( Figure  3a). The efficiency of the oxygen evolving complex on the donor side of PSII (Fv/Fo) in tomato leaves after 72 h of treatment by 1 mM SA increased compared to the control plants (sprayed by distilled water) (Figure 3b).

The Spatial Pattern of Photosystem II Activity of Control and Salycilic Acid Treated Tomato Leaves
The whole tomato leaf pattern after 72 h of treatment by 1 mM SA is shown in Figure  4, with the parameter of the minimum chlorophyll a fluorescence in the dark (Fo) to decrease, while the maximum chlorophyll a fluorescence in the light (Fm') to increase ( Figure  4).

The Spatial Pattern of Photosystem II Activity of Control and Salycilic Acid Treated Tomato Leaves
The whole tomato leaf pattern after 72 h of treatment by 1 mM SA is shown in Figure 4, with the parameter of the minimum chlorophyll a fluorescence in the dark (Fo) to decrease, while the maximum chlorophyll a fluorescence in the light (Fm') to increase ( Figure 4).  The maximum efficiency of PSII photochemistry (Fv/Fm) increased significantly after 72 h of treatment by 1 mM SA, while the non-photochemical quenching (NPQ), depicted as NPQ/4, decreased compared to tomato leaflets sprayed with distilled water (control) ( Figure 4). The effective quantum yield of photochemistry (ΦPSII) increased significantly after 72 h of treatment by 1 mM SA, while the quantum yield of regulated non-photochemical energy loss (ΦNPQ) decreased to such an altitude that the quantum yield of non- The maximum efficiency of PSII photochemistry (Fv/Fm) increased significantly after 72 h of treatment by 1 mM SA, while the non-photochemical quenching (NPQ), depicted as NPQ/4, decreased compared to tomato leaflets sprayed with distilled water (control) (Figure 4). The effective quantum yield of photochemistry (Φ PSII ) increased significantly after 72 h of treatment by 1 mM SA, while the quantum yield of regulated non-photochemical energy loss (Φ NPQ ) decreased to such an altitude that the quantum yield of non-regulated energy (Φ NO ) remained constant compared to tomato leaflets sprayed with distilled water (control) ( Figure 5). The fraction of open PSII reaction centers (qp) increased significantly after 72 h of treatment by 1 mM SA compared to the control ( Figure 5). Representative color-coded images of the minimum chlorophyll a fluorescence in the dark (Fo), the maximum chlorophyll a fluorescence in the light (Fm'), the maximum efficiency of PSII photochemistry (Fv/Fm), and the non-photochemical quenching (NPQ), depicted as NPQ/4, of tomato leaflets, after 72 h of foliar spray by either distilled water (control) or 1 mM salicylic acid (SA). The areas of interest (AOI) are shown in circles and the resulting values for each chlorophyll fluorescence parameter for the whole leaflet (average ± SD) are given for the dark adaptive state or under 205 μmol photons m −2 s −1 corresponding to low light intensity (LL). The color code showed at the bottom ranges from values 0.0 to 1.0. An asterisk indicates statistically significant difference (p < 0.05) between the control and 1 mM salicylic acid (SA) treatment.
The maximum efficiency of PSII photochemistry (Fv/Fm) increased significantly after 72 h of treatment by 1 mM SA, while the non-photochemical quenching (NPQ), depicted as NPQ/4, decreased compared to tomato leaflets sprayed with distilled water (control) (Figure 4). The effective quantum yield of photochemistry (ΦPSII) increased significantly after 72 h of treatment by 1 mM SA, while the quantum yield of regulated non-photochemical energy loss (ΦNPQ) decreased to such an altitude that the quantum yield of nonregulated energy (ΦNO) remained constant compared to tomato leaflets sprayed with distilled water (control) ( Figure 5). The fraction of open PSII reaction centers (qp) increased significantly after 72 h of treatment by 1 mM SA compared to the control ( Figure 5).

Reactive Oxygen Species Imaging of Control and Salicylic Acid Treated Tomato Leaves
Reactive oxygen species imaging, which was performed with 25 µM 2', 7'-dichlorofluorescein diacetates in the dark, revealed a decrease in ROS generation in 1 mM SA of tomato leaflets after 72 h of spray (Figure 6b) compared to tomato leaflets sprayed by distilled water (control) (Figure 6a). A higher ROS generation was visible in control leaves, as green fluorescence localized mainly in leaf veins and leaf hairs (Figure 6a).

Reactive Oxygen Species Imaging of Control and Salicylic Acid Treated Tomato Leaves
Reactive oxygen species imaging, which was performed with 25 µ M 2', 7'-dichlorofluorescein diacetates in the dark, revealed a decrease in ROS generation in 1 mM SA of tomato leaflets after 72 h of spray (Figure 6b) compared to tomato leaflets sprayed by distilled water (control) (Figure 6a). A higher ROS generation was visible in control leaves, as green fluorescence localized mainly in leaf veins and leaf hairs (Figure 6a).

Discussion
Salicylic acid (SA), introduced as the "sixth" principal phytohormone only in the early 1990s [19], is one of the key hormones of plant disease resistance [50,51] and can also directly or indirectly affect various physiological processes, including photosynthesis [20].

Discussion
Salicylic acid (SA), introduced as the "sixth" principal phytohormone only in the early 1990s [19], is one of the key hormones of plant disease resistance [50,51] and can also directly or indirectly affect various physiological processes, including photosynthesis [20]. Experimental evidence showed that SA exerts inhibitory effects on chlorophyll content, stomatal function, photosynthetic parameters, and carboxylating enzymes, playing a regulatory role associated with photosynthetic reactions [52]. SA takes part in plant responses to many abiotic stresses such as drought, salinity, chilling, heat, and heavy metal toxicity [10,11,[53][54][55][56][57][58]. However, since its mode of action depends significantly on numerous aspects, such as the plant species, the environmental conditions, the concentration used, the duration of exposure, and the concentrations of other external agents [4,20,59], data on exogenously applied SA on plant physiological processes under stress or non-stress conditions remain controversial [20].
The fundamental organelles in photosynthesis are the chloroplasts that are composed of thylakoids, in which reside the antenna of the light-harvesting complexes (LHCI, LHCII) that capture the light energy by the photosynthetic pigments, including chlorophylls [60]. Chlorophyll molecules are the main pigments in absorbing light quanta and transferring the energy to the reaction centers where charge separation happens and electron transport begins [40,61]. In case the absorbed light energy exceeds the capacity of photosynthesis to use it for assimilation, generation of high levels of ROS occur [61][62][63]. Although excess light is potentially detrimental, plants have developed several mechanisms to cope with the excess absorbed energy to prevent photooxidative stress [61][62][63]. Photooxidative stress results in photodamage, either at the acceptor side through triplet excited state chlorophylls ( 3 Chl*), resulting in singlet oxygen ( 1 O 2 ) formation, or at the donor side through inactivation of the oxygen evolving complex (OEC) [64]. Plants possess limited capability to regulate the amount of absorbed sunlight through changes in leaf angle, leaf area, chloroplast movement, and, on a molecular level, through acclimatory adjustments of antenna size [2].
Plants with higher chlorophyll content have larger antenna size and can capture and absorb more light energy [65]. On the contrary, plants with reduced chlorophyll content have smaller chlorophyll antenna size, representing reduced NPQ and ROS generation [65]. Decreasing leaf chlorophyll concentration has been suggested as a potential method to reduce excess absorption of sunlight and improve photosynthetic efficiency [1,2,[65][66][67]. Improved photosynthetic efficiency is achieved via a better allocation of absorbed light energy that also reduces photooxidative stress [68].
When mustard leaves were sprayed with 0.01 mM SA, the chlorophyll content was significantly enhanced, whereas higher SA concentrations lessened chlorophyll content [69,70]. In barley and black gram plants pre-treated with SA, a decrease in chlorophyll content was detected [71,72]. In our experiment, the higher chlorophyll content observed in control leaves (Figure 1a) was correlated with lower values of maximal fluorescence yield at the light-adapted state (Fm') ( Figure 4), as has also been pointed out previously [73]. These lower values of Fm' were corelated with higher photoprotective energy dissipation as heat (NPQ) in control leaves compared to SA treated (Figure 4), indicating photoprotective effects provided by the foliar application of SA. Photoprotection by NPQ is important for optimal growth and development [74,75]. A lower value of minimal fluorescence yield at the dark-adapted state (Fo), as we noticed in SA treated tomato leaves (Figure 4), was shown to indicate photoprotective effects provided by anthocyanins in the red leaf parts [73]. In accordance with this, tomato plants, grown in non-stressed conditions and water sprayed (control), reached an NPQ value of~0.7 at 205 µmol photons m −2 s −1 , while 1 mM SA sprayed reached a lower amplitude (~0.5) (Figure 1f). This indicates that in SA sprayed leaves there was lesser photoprotective need. Still, the decreased NPQ in SA sprayed leaves indicates that its action minimizes the potential for photooxidative damage. Additionally, the lower value of minimal fluorescence yield at the dark-adapted state (Fo) in SA sprayed leaves has been shown to be correlated with photoprotection [73].
Salicylic acid was shown to slow down PSII electron transport rate (ETR) [76] but according to our results, tomato plants sprayed with SA showed an enhancement of ETR and increased effective quantum yield of photosystem II (PSII) photochemistry (Φ PSII ) at both LL and HL (Figure 1c). The increased quantum yield of PSII photochemistry (Φ PSII ) according to Genty et al. [77] can be attributed either to the increased fraction of open PSII reaction centers (qp), which is a measure of the redox state of quinone A (Q A ), or to the increased efficiency of these centers (Fv'/Fm'). In our case, the increased Φ PSII was due to both the increased fraction of open PSII reaction centers (Figure 2a) and to the increased efficiency of these centers (Figure 2b). In SA sprayed leaves, a larger amount of absorbed light energy was distributed to PSII photochemistry, as revealed by the significant increase in Φ PSII (Figure 1c) and ETR (Figure 2c).
Salicylic acid (1 mM), supplied in the nutrient solution of tomato plants for 24 h, decreased the maximum efficiency of PSII (Fv/Fm), and the effective quantum yield of PSII (Φ PSII ) [78], but in our experiment on SA sprayed leaves, we observed the reverse effects, with SA resulting in increased Fv/Fm and Φ PSII values (Figure 1b,c). Lower Fv/Fm values, as observed in distilled water (control) sprayed tomato leaves (Figure 1b), indicate a higher degree of photoinhibition [12,14]. It seems that most results obtained with exogenous SA cannot be generalized, since the effect may differ not only with plant species, but it may vary on the method of SA application (foliar spray, growth medium addition, seed pre-soaking, etc.), as well as on the experimental duration time [76].
Despite the lower chlorophyll content of SA-treated leaves (Figure 1a), the absorbed light energy was preferentially converted into photochemical energy (Figure 1c) rather than dissipated as thermal energy by NPQ (Figure 1f). The on-photochemical quenching (NPQ) mechanism can reduce the energy transfer to reaction centers [30] and represents mainly the thermal energy dissipation from LHCII via the zeaxanthin quencher [40,79,80]. The increased heat dissipation by de-excitation (NPQ) in control plants (Figure 1f) decreases the efficiency of photochemical reactions of photosynthesis (Figure 1c) (downregulation of PSII) [30,44,74,[81][82][83]. Thus, the decreased non-photochemical quenching (NPQ) (Figure 1f) and relative excess energy at PSII (EXC) (Figure 3a) in SA treated leaves indicated improvements related to PSII efficiency. Photosystem II responses triggered by the NPQ mechanism is an approach to protect the photosynthetic apparatus from photo-oxidative damage by dissipating excess light energy such as heat at PSII [48,[84][85][86], regulating photosynthetic ETR [87,88] and avoiding detrimental ROS creation [48,81]. The downregulation of PSII is a reversible decrease in the quantum yield of PSII under excess light conditions. Photoinhibition, also called photoinactivation, lowers the number of photochemically active PSII centers and recovery occurs only via degradation and resynthesis of the D1 protein of the photoinhibited PSII centers [16]. SA has been previously reported to act as a signal molecule to activate chlorophyll catabolic genes [32]. Our results point out that an SA stimulated decrease in chlorophyll content can be regarded as a mechanism that can lower photoinhibition and photodamage of PSII, while the blockage of a decrease in chlorophyll content intensifies PSII photoinhibition and photodamage [32].
Increasing concentrations of SA (0.1 mM, 0.5 mM and 1 mM), imposed through the root medium for a period of 7 day on barley (Hordeum vulgare L.), caused a concentration dependent inhibitory effect on PSII function, by inhibiting the electron donation from the oxygen evolving complex (OEC) to PSII reaction centers, as a result of some structural changes in the catalytic Mn cluster at protein level, resulting in a decrease in the fraction of open of PSII centers [52]. In contrast, in our experiment, a lower efficiency of the oxygen evolving complex (OEC) was noticed in the untreated (control) tomato leaves, while foliar application of SA resulted to enhanced efficiency of the OEC (Figure 3b) and to an increased fraction of open of PSII reaction centers (Figure 2a). Thus, in addition to the concentration of SA used; the choice of application method is critical for its impact on a plant's physiology. The main site of NPQ is the marginal PSII antenna [86] but quenching by the reaction centers is also probable [89], especially under a low OEC efficiency [83], possibly explaining the increased NPQ at LL in the untreated (control) tomato leaves (Figure 1f). The decreased efficiency of the OEC is associated with the formation of H 2 O 2 , which can be further oxidized to the superoxide radical (O 2 • −) [90]. This could explain the increased ROS formation in control leaves compared to SA-treated leaves ( Figure 6).
When the rate of excitation energy reaching PSII is higher than can be used in photochemistry, this leads to increased "excitation pressure" (1 − qp) on PSII, which can be measured non-invasively using the saturating light pulse method of chlorophyll fluorescence [90]. Foliar application of 1 mM SA on tomato leaves decreased the excitation pressure at PSII (1 − qp), compared to the untreated one (Figure 2d), also decreasing the excess excitation energy (EXC) (Figure 3a). The conversion efficiency of absorbed light energy to photochemical energy is critical in describing plant productivity over time [33].
When plants are under a HL environment, avoiding photosynthetic yield decline is considered critical [91]. Photosystem II over-excitation increases the conversion of unquenched singlet excited state chlorophylls ( 1 Chl*) into triplet excited state chlorophylls ( 3 Chl*), accompanied by 1 O 2 generation that damages and inhibits PSII [29,39,40,64,[91][92][93]. The role of 1 O 2 is ambiguous; under moderate stress, it acts as a retrograde signal, whereas under severe stress, it causes oxidative damage [94]. Under HL, both control and SA treated plants showed an equal maximum value in the quantum efficiency of thermal dissipation (Φ NPQ ) (Figure 1d), and a minimum value in Φ PSII , which was even lower in the control than SA-treated plants (Figure 1c). As a result, under HL, control plants displayed higher values of non-regulated energy dissipated in PSII (Φ NO ) than SA-treated plants (Figure 1e). A decreased Φ NO at HL by the SA treatment compared to controls (Figure 1e) implies a better photoprotection and is indicative of lower 1 O 2 production [95]. The inability of PSII to utilize the absorbed light energy for photochemistry (Φ PSII ), or to safely dissipate it as heat (Φ NPQ ), can lead to the formation of a triplet chlorophyll state ( 3 Chl*) that can react with O 2 to produce the very reactive 1 O 2 [93,95]. The probability of formation of 3 Chl* can be calculated by Φ NO [96]. An increased Φ NO reflects the inability of a plant to protect itself against damage by excess illumination, which will eventually lead to photodamage [97,98], indicating that the plant has problems coping with the incident HL [95].
Singlet oxygen ( 1 O 2 ) produced by the triplet excited state chlorophylls ( 3 Chl*) can further produce the other ROS, e.g., O 2 • − and H 2 O 2 [32,99]. However, electron leakage to O 2 at PSI, resulting in O 2 • −, which is converted to H 2 O 2 , is the main pathway of H 2 O 2 generation [39,44,45]. Increased 1 O 2 generation is considered to be harmful, since it can inhibit the repair of PSII reaction centers and/or contribute directly in oxidation and PSII damage, or even more activate programmed cell death [39,63,87,93,98,99]. Singlet oxygen production is considered to act as the agent of photo-inhibitory damage [16].
Among the ROS, 1 O 2 and H 2 O 2 initiate diverse signaling networks when photosynthesis is disturbed [100]. Singlet oxygen, since it is very reactive, initiates but does not transduce signaling. On the other hand, H 2 O 2 , due to its lower reactivity, is a mobile messenger in a spatially defined signaling pathway [100]. Foliar produced chloroplastic H 2 O 2 shows preferential accumulation in leaf bundle sheath cells [100][101][102][103] (Figure 6). Hydrogen peroxide can be transmitted throughout the leaf veins to act as a long-distance signaling molecule [99,103].
Tomato leaves sprayed with 1 mM SA with a lower chlorophyll content compared to water sprayed (Figure 1a) are characterized by decreased non-regulated energy dissipated in PSII (Φ NO ) under high light treatment (Figure 1e), suggesting a lower amount of triplet excited state chlorophyll ( 3 Chl*) molecules available to produce singlet oxygen ( 1 O 2 ). The decreased chlorophyll content in tomato plants stimulated by SA treatment can be regarded as a mechanism that can lower the photoinhibition and photodamage of PSII [1,2], and has been regarded as a way to suppress phototoxicity in plants [32,104]. Block of chlorophyll catabolism exacerbates PSII photoinhibition and photodamage [32]. The molecular mechanism by which, in SA-treated leaves with lower chlorophyll content, the absorbed light energy is more efficiently directed to photochemistry, was the increased efficiency of the reaction centers (Fv'/Fm') (Figure 2b), and the increased fraction of open PSII reaction centers (qp) (Figure 2a), which enhanced ETR (Figure 2c).
Tomato leaves sprayed with 1 mM SA presented a decrease in chlorophyll content associated with higher PSII photochemistry (Φ PSII ) and electron transport rate (ETR), but also with lower excitation pressure (1 − qp) and lower excess excitation energy (EXC). 1 mM SA sprayed tomato leaves also possessed a higher capacity to maintain low Φ NO under HL (Figure 1e), reflecting the ability of SA sprayed tomato leaves to be protected against damage from excess illumination.
The molecular mechanism by which SA can induce the decrease in chlorophyll content under optimum growth conditions may be a field worth further investigation. How this chlorophyll loss occurred may differ from the pathway of chlorophyll catabolism during leaf senescence in essential aspects of gene regulation and biochemistry.

Salicylic Acid Treatment
Tomato (Lycopersicon esculentum Mill. cv. Meteor) plants were sprayed either with distilled water (control) or with 1 mM salicylic acid (SA). All treatments were performed with four independent biological replicates.

Chlorophyll Content
The relative chlorophyll content of the control (sprayed with distilled water) or 1 mM SA-treated leaves for 72 h was measured photometrically with a portable Chlorophyll Content Meter (Model Cl-01, Hansatech Instruments Ltd., Norfolk, UK), using dual wavelength optical absorbance (620 and 920 nm) and expressed in relative units [73].

Chlorophyll Fluorescence Analysis
Chlorophyll fluorescence measurements were performed in dark-adapted (20 min) tomato control and SA sprayed plants using an Imaging-PAM Fluorometer M-Series MINI-Version (Heinz Walz GmbH, Effeltrich, Germany). Chlorophyll fluorescence analysis was performed as described in detail previously [105]. Measurements were conducted on tomato leaves after 72 h of foliar spray, either by distilled water (control) or 1 mM SA. Six to nine areas of interest (AOI) were selected in each leaflet. For each AOI, we measured the minimum (Fo) and the maximum (Fm) chlorophyll a fluorescence in the dark. The maximum chlorophyll a fluorescence in the light (Fm'), with saturating pulses (SPs was computed every 20 s for 5 min after application of the actinic light (AL), while the minimum chlorophyll a fluorescence in the light (Fo') was computed as Fo' = Fo/(Fv/Fm + Fo/Fm') [106], where Fv is the variable chlorophyll a fluorescence (in the dark-adapted leaves), calculated as Fm − Fo. Steady-state photosynthesis (Fs) was measured after 5 min of illumination time, either with AL of 205 µmol photons m −2 s −1 corresponding to low light intensity (LL), or with 1000 µmol photons m −2 s −1 corresponding to high light intensity (HL). By using Win software (Heinz Walz GmbH, Effeltrich, Germany), we estimated the maximum efficiency of PSII photochemistry (Fv/Fm), the effective quantum yield of PSII photochemistry (Φ PSII ), the quantum yield of regulated non-photochemical energy loss (Φ NPQ ), and the quantum yield of non-regulated energy (Φ NO ), according to Krammer et al. [49]. The efficiency of the oxygen evolving complex (OEC) on the donor side of PSII (Fv/Fo) [30,48,107,108], the efficiency of excitation energy capture by open PSII centers (Fv'/Fm') [77], the redox state of quinone A (Q A ), representing the fraction of open PSII reaction centers qp = (Fm'− Fs)/(Fm' − Fo') [77], and the non-photochemical quenching (NPQ), reflecting the dissipation of excitation energy as heat were calculated as (Fm − Fm')/Fm' [109]. The electron transport rate (ETR), calculated as Φ PSII × PAR × c × abs, where PAR is the pho-tosynthetically active radiation, c is 0.5, and abs is the total light absorption of the leaf taken as 0.84 [110], the excitation pressure (1 − qp), and the excess excitation energy (EXC) as (Fv/Fm − Φ PSII )/(Fv/Fm) [111] were also calculated.
Representative results as color-coded images of the minimum chlorophyll a fluorescence in the dark (Fo), the maximum chlorophyll a fluorescence in the light (Fm'), the maximum efficiency of PSII photochemistry (Fv/Fm), the non-photochemical quenching (NPQ), depicted as NPQ/4, the effective quantum yield of PSII photochemistry (Φ PSII ), the quantum yield of regulated non-photochemical energy loss (Φ NPQ ), the quantum yield of non-regulated energy (Φ NO ), and the fraction of open PSII reaction centers (qp), are also shown.

Imaging of Reactive Oxygen Species
Imaging of ROS in tomato leaflets was performed after 72 h of foliar spray, either by distilled water (control) or 1 mM SA, as described previously [112]. ROS specific fluorescence in tomato leaflets were observed with a Zeiss AxioImager Z2 epi-fluorescence microscope, equipped with an AxioCam MRc5 digital camera [113]. Leaves were previously incubated with 25 µM 2 , 7 -dichlorofluorescein diacetate (DCF-DA, Sigma Aldrich, Chemie GmbH, Schnelldorf, Germany) for 30 min in the dark [114].

Statistics
Pairwise differences in the control and SA treatments were analyzed with independent samples t-test for unequal variances, using the IBM SPSS Statistics for Windows version 28 at a p < 0.05 level.

Conclusions
We conclude that foliar applied SA induced a decrease in chlorophyll content, lowering ROS generation and enhancing PSII efficiency. However, the impact of SA on plant growth and physiology relies highly on the study design strategy and depends significantly on numerous aspects, such as the plant species or the genotype, the environmental conditions, the concentration used, the duration of exposure, and, mainly, the method of application (foliar, rooting medium, e.g., soil or hydroponic). According to our experimental data, foliar application of 1 mM SA by spray, compared to other application methods, has the best beneficial effect, alleviating photoinhibition and photodamage and improving PSII efficiency in the crop plant, tomato.
This study sheds light on the role that chlorophyll molecules play in the regulation of PSII efficiency, as well as on ROS generation. The results extend our mechanistic understanding of SA-induced enhancement of PSII efficiency through a decreased chlorophyll content that suppressed phototoxicity, thus it can be regarded as a mechanism that lowers photoinhibition and photodamage of PSII in the important horticultural crop, tomato.