A Novel Triple-Action Inhibitor Targeting B-Cell Receptor Signaling and BRD4 Demonstrates Preclinical Activity in Chronic Lymphocytic Leukemia

B-cell chronic lymphocytic leukemia (CLL) results from intrinsic genetic defects and complex microenvironment stimuli that fuel CLL cell growth through an array of survival signaling pathways. Novel small-molecule agents targeting the B-cell receptor pathway and anti-apoptotic proteins alone or in combination have revolutionized the management of CLL, yet combination therapy carries significant toxicity and CLL remains incurable due to residual disease and relapse. Single-molecule inhibitors that can target multiple disease-driving factors are thus an attractive approach to combat both drug resistance and combination-therapy-related toxicities. We demonstrate that SRX3305, a novel small-molecule BTK/PI3K/BRD4 inhibitor that targets three distinctive facets of CLL biology, attenuates CLL cell proliferation and promotes apoptosis in a dose-dependent fashion. SRX3305 also inhibits the activation-induced proliferation of primary CLL cells in vitro and effectively blocks microenvironment-mediated survival signals, including stromal cell contact. Furthermore, SRX3305 blocks CLL cell migration toward CXCL-12 and CXCL-13, which are major chemokines involved in CLL cell homing and retention in microenvironment niches. Importantly, SRX3305 maintains its anti-tumor effects in ibrutinib-resistant CLL cells. Collectively, this study establishes the preclinical efficacy of SRX3305 in CLL, providing significant rationale for its development as a therapeutic agent for CLL and related disorders.


Introduction
Chronic lymphocytic leukemia (CLL) is characterized by the expansion of mature B-cells that accumulate in the blood, bone marrow (BM), and secondary lymphoid organs such as the lymph node (LN) and spleen. Hallmark features of CLL include amplified B-cell receptor (BCR) signaling, defective apoptosis, profound tumor microenvironment (TME) dependency, and immune dysfunction [1,2]. Novel therapies targeting BCR signaling (e.g., ibrutinib and idelalisib) and B-cell leukemia/lymphoma-2 (BCL-2) family proteins (e.g., venetoclax) provide significantly improved outcomes for both previously untreated idelalisib in primary CLL/nurse-like cell co-cultures reflective of the LN TME in CLL [32]. Simultaneous targeting of different components of BCR signaling and BET proteins may permit less opportunity for resistance mechanisms to develop, which could translate into more durable patient responses and disease control.
We recently introduced thienopyranone (TP) scaffold-based chemotypes [36] for the combinatorial inhibition of BTK, PI3K-AKT, and BRD4-MYC in a single compound (i.e., SRX3262 and SRX3305) and demonstrate impressive preclinical activity in mantle cell lymphoma (MCL) [37,38]. BTK/PI3K/BRD4 triple-inhibitors present a potential solution to the toxicity that often plagues combination therapeutic strategies that are necessary to overcome CLL progression and drug resistance. Importantly, our studies demonstrated that these novel multi-action inhibitors yield potent anti-tumor effects but spare healthy bystander cells and are less toxic to healthy donor B-cells than the combination of single-target drugs required to effectively inhibit the same three targets. In this study, we extend our evaluation of SRX3305 to include preclinical models of CLL and DLBCL, including ibrutinibresistant CLL, further validating the efficacy of a multi-target single-molecule approach to overcome TME-and drug-induced resistance mechanisms in B-cell malignancies.

SRX3305 Inhibits Proliferation and Induces Apoptosis of Malignant B-Cell Lines
The anti-proliferative properties of SRX3305 or SRX3262 (first-generation BTK/PI3K/BRD4 triple-inhibitor) were first evaluated in a panel of B-cell non-Hodgkin lymphoma (B-NHL) cell lines representative of CLL (OSU-CLL, HG-3, MEC-1, and MEC-2) and DLBCL (OCI-LY3 and SU-DHL-6). Key cell line characteristics/genetic aberrations are summarized in Supplementary Table S1 [39][40][41][42][43][44][45][46][47]. SRX3305 and SRX3262 significantly inhibited CLL cell proliferation in a dose-dependent manner ( Figure 1A-D). We report an average IC 50 of 1.38 µM for SRX3305 and 2.98 µM for SRX3262 across the CLL lines evaluated. SRX3305 appeared to be superior to SRX3262 (on average 2.5-fold more potent, p < 0.05) and hence was used for all further studies. SRX3305 is at least equipotent to common single-target inhibitors (ibrutinib, idelalisib, JQ1, or OTX015) evaluated on this panel of B-NHL cells. Similarly, SRX3305 reduced cell proliferation in DLBCL cells (Supplementary Figure S1) with IC 50 values of~290 nM in OCI-LY3 cells (activated B-cell DLBCL subtype) and 920 nM in SU-DHL-6 cells (germinal center DLBCL subtype). We next demonstrate that SRX3305 induces apoptosis in HG-3 and OSU-CLL cells in a dose-dependent manner at 24 h ( Figure 1E,F), and a similar trend was witnessed at 48 h (data not shown). This indicates that SRX3305 not only induces a cytostatic effect, but also a cytotoxic effect in CLL cells.  . Results are given as mean ± SEM. Asterisks denote significant inhibitor-induced apoptosis vs. control vehicle. * p < 0.05, ** p < 0.01, *** p < 0.001.

SRX3305 Inhibits Critical BCR Survival Signaling in CLL
Two major targets of SRX3305, BTK and PI3K, are vital to BCR survival and proliferation signaling [48]. The third, BRD4, is critical for the transcriptional regulation of numerous factors driving CLL pathogenesis, including MYC [23,30]. We show that SRX3305 effectively inhibits the phosphorylation of BTK and PRAS40 (indicative of PI3K/AKT signaling) and reduces MYC expression in OSU-CLL, MEC-1 and MEC-2 cells (Figure 2A). Single-target inhibitors (ibrutinib, idelalisib, JQ1) were used as controls.
Next, we sought to examine the effects of SRX3305 under activation-induced BCR signaling. Following inhibitor treatment for 4 h, OSU-CLL and MEC-1 cells were stimulated with anti-IgM in the last 15 min. In parallel, inhibitor washout experiments were conducted to further evaluate whether inhibitor-induced target modulation could be maintained. CLL cells were subject to inhibitor treatment for 1 h prior to inhibitor washout. Three hours later, the cells were stimulated with anti-IgM (last 15 min) to elicit BCR crosslinking. In these studies, ibrutinib, a covalent irreversible BTK inhibitor, and the noncovalent reversible BTK inhibitor BMS-935177 were used as controls. As expected, continuous inhibitor treatment resulted in decreased phosphorylation of BTK (p-BTK) and PRAS40 (p-PRAS40) and reduced MYC expression in BCR-activated CLL cells. CLL cells treated with SRX3305 or ibrutinib (covalent BTK inhibitor) also retained the inhibition of p-BTK following treatment washout, while cells treated with the reversible inhibitor BMS935177 regained BTK phosphorylation. Notably, SRX3305-treated cells maintained marked inhibition of p-PRAS40 and MYC expression after treatment washout ( Figure 2B), whereas these effects were lost in ibrutinib-treated cells. In comparison to OSU-CLL cells, MEC-1 cells that harbor del(17p) (Supplementary Table S1) appeared less sensitive to maintained SRX3305-mediated downregulation of p-PRAS40 in the washout condition. This may be due to reduced sensitivity of del(17p) CLL cells to PI3K/AKT inhibition as previously reported [49]. In spite of the modest modulation of p-PRAS40 in the MEC-1 washout conditions, SRX3305 still maintained prominent modulation of other oncogenic factors (p-BTK, MYC), indicating the therapeutic benefit of this triple-inhibition in high risk del(17p) CLL. irreversible BTK inhibitor), or 1 µM BMS-986142 (BMS; reversible BTK inhibitor). Cells were either incubated with treatment continuously for 4 h (Continuous Tx), or treatment was washed out after 1 h and replaced with fresh media for the remaining 3 h of incubation (Wash-out Tx). The stimulation of BCR signaling was performed via the addition of 10 µg/mL anti-IgM for the last 15 min (+ɑ-IgM). Whole-cell lysates were then analyzed for expression or phosphorylation of the indicated proteins (n = 3 independent experiments per cell line). β-Tubulin serves as a loading control. Representative immunoblots are shown for each cell line. Numbers below the bands represent densitometric quantification relative to loading control, total protein (for phosphorylated proteins), and normalized to Veh (for (A)) or +α-IgM Veh (for (B)).
Next, we sought to examine the effects of SRX3305 under activation-induced BCR signaling. Following inhibitor treatment for 4 h, OSU-CLL and MEC-1 cells were stimulated with anti-IgM in the last 15 min. In parallel, inhibitor washout experiments were conducted to further evaluate whether inhibitor-induced target modulation could be maintained. CLL cells were subject to inhibitor treatment for 1 h prior to inhibitor washout. . Cells were either incubated with treatment continuously for 4 h (Continuous Tx), or treatment was washed out after 1 h and replaced with fresh media for the remaining 3 h of incubation (Wash-out Tx). The stimulation of BCR signaling was performed via the addition of 10 µg/mL anti-IgM for the last 15 min (+α-IgM). Whole-cell lysates were then analyzed for expression or phosphorylation of the indicated proteins (n = 3 independent experiments per cell line). β-Tubulin serves as a loading control. Representative immunoblots are shown for each cell line. Numbers below the bands represent densitometric quantification relative to loading control, total protein (for phosphorylated proteins), and normalized to Veh (for (A)) or +α-IgM Veh (for (B)).

SRX3305 Inhibits Primary Malignant B-Cell Survival and Proliferation
To mimic conditions within pseudo-proliferation centers where CLL expands [50], we evaluated SRX3305 in primary CLL cells stimulated with CpG oligodeoxynucleotides (CpG ODN), a well-known Toll-like receptor 9 agonist that promotes ex vivo CLL cell proliferation [51]. Characteristics of the patients' samples used are summarized in Supplementary Table S2. SRX3305 markedly decreased CpG ODN-induced proliferation of primary CLL cells in a dose-dependent manner ( Figure 3A). Immunoblot analysis showed that SRX3305 reversed CpG ODN-mediated proliferation reflected by reduced MYC levels in primary CLL cells ( Figure 3B). In parallel, SRX3305 induced the accumulation of P21 (cyclin-dependent kinase inhibitor), indicative of cell cycle arrest [52] ( Figure 3B). SRX3305 did not influence the proliferation of patient-derived CLL cells under unstimulated/basal conditions (data not shown).   We next investigated SRX3305-induced cytotoxicity on tumor cells derived from murine models of aggressive B-cell leukemia and lymphoma. We utilized malignant B-cells isolated from Eµ-TCL1 [53,54] (CLL model) and Eµ-Myc/TCL1 mice [55,56] (concurrent CLL and B-cell lymphoma model). Primary murine spleen-derived tumor samples (comprising >90% malignant B-cells) were cultured ex vivo under mitogenic stimulation (PMA/ionomycin). SRX3305 treatment induced significant cytotoxicity in Eµ-TCL1 and Eµ-Myc/TCL1-derived malignant cells in a dose-dependent manner ( Figure 3C), suggesting promising therapeutic benefits in aggressive CLL and associated lymphomas (e.g., DLBCL-RT). As expected, SRX3305 was more effective that the single-target inhibitors (ibrutinib, idelalisib, JQ1) in primary malignant B-cells.

SRX3305 Disrupts Stroma Survival Support and Chemokine-Induced Migration in CLL
Given the importance of the TME in CLL pathogenesis and therapy resistance [57,59,59], we sought to evaluate the efficacy of SRX3305 in the presence of stroma protection. The clinical efficacy of inhibitors targeting BCR signaling is partially attributed to disrupting CLL-cell interactions with its TME [60]. As expected, the co-culture of primary CLL cells on BM-derived stromal cells protects them from spontaneous apoptosis [59], resulting in a significant increase in CLL cell viability. Notably, treatment with SRX3305 reduced CLL cell viability in a dose-dependent manner despite stroma protection ( Figure 4A). This was comparable to CLL therapeutics, ibrutinib, or idelalisib. Of note, SRX3305 was not toxic to the stromal cells (Supplementary Figure S2), indicating that SRX3305-induced CLL cell cytotoxicity is not a function of reduced stroma viability.
CLL cell migration, homing to supportive TME niches, and retention therein have been reported to support leukemic cell survival and disease progression [61,62]. We therefore investigated if SRX3305 could inhibit CLL cell migration towards key chemokines secreted by protective stromal cells in the BM (CXCL-12) and secondary lymphoid tissues (CXCL-13) [63][64][65]. Using a trans-well assay system, we demonstrate that SRX3305 reduced the migration of MEC-1 cells towards CXCL-12 or CXCL-13 ( Figure 4B,C), further indicating that SRX3305 can disrupt CLL cell trafficking to supportive niches. Similar findings were seen in preclinical studies of the highly active BCR-targeting CLL therapeutic ibrutinib [4,66]. These findings suggest that SRX3305 can overcome TME-induced survival signaling, an established mediator in resistance to therapy in CLL.
duced CLL cell viability in a dose-dependent manner despite stroma protection 4A). This was comparable to CLL therapeutics, ibrutinib, or idelalisib. Of note, S was not toxic to the stromal cells (Supplementary Figure S2), indicating that S induced CLL cell cytotoxicity is not a function of reduced stroma viability. CLL cell migration, homing to supportive TME niches, and retention there been reported to support leukemic cell survival and disease progression [62,63]. W fore investigated if SRX3305 could inhibit CLL cell migration towards key che secreted by protective stromal cells in the BM (CXCL-12) and secondary lymphoi (CXCL-13) [64][65][66]. Using a trans-well assay system, we demonstrate that SRX duced the migration of MEC-1 cells towards CXCL-12 or CXCL-13 ( Figure 4B,C) indicating that SRX3305 can disrupt CLL cell trafficking to supportive niches.

SRX3305 Is Active in Ibrutinib-Resistant CLL
We hypothesized that because SRX3305 selectively targets PI3K and BRD4 in addition to BTK, this inhibitor would remain effective against ibrutinib-resistant CLL. To evaluate this in the context of non-genetic acquired drug resistance, we generated an ibrutinibresistant CLL cell line model by prolonged culture of HG-3 cells with increasing amounts of ibrutinib (Supplementary Figure S3). Remarkably, SRX3305 decreased cell proliferation in ibrutinib-resistant HG3 (IR-HG3) cells ( Figure 5A), while the anti-proliferative effects of ibrutinib were attenuated in IR-HG3 cells. These data suggest that SRX3305 has the potential to overcome acquired ibrutinib resistance. Immunoblot analysis revealed that SRX3305 consistently decreased MYC expression and p-PRAS40 (PI3K target) and increased P21 levels in IR-HG3 cells, while ibrutinib demonstrated a partial response and failed to reduce p-PRAS40 ( Figure 5B). Overall, BTK activation (phosphorylation) is reduced in the IR-HG3 cell line, suggesting that adaptive kinome reprogramming bypasses the effect of ibrutinib and an increased reliance on alternative survival mechanisms such as PI3K/AKT/ERK [7,67]. Hence, as expected, we observed minimal decrease in BTK phosphorylation upon treatment with SRX3305 or ibrutinib. creased P21 levels in IR-HG3 cells, while ibrutinib demonstrated a partial response and failed to reduce p-PRAS40 ( Figure 5B). Overall, BTK activation (phosphorylation) is reduced in the IR-HG3 cell line, suggesting that adaptive kinome reprogramming bypasses the effect of ibrutinib and an increased reliance on alternative survival mechanisms such as PI3K/AKT/ERK [7,68]. Hence, as expected, we observed minimal decrease in BTK phosphorylation upon treatment with SRX3305 or ibrutinib. or GAPDH were used as loading controls. Numbers below the bands represent densitometric quantification relative to loading control, total protein (for phosphorylated proteins), and normalized to +α-IgM Veh. Representative immunoblots are shown for the phosphorylation and expression of the indicated proteins. HSP90 or GAPDH were used as loading controls. Numbers below the bands represent densitometric quantification relative to loading control, total protein (for phosphorylated proteins), and normalized to +α-IgM Veh.

Discussion
Aberrant activities of diverse signaling pathways, including BTK, PI3K-AKT, and MYC-BRD4, contribute to CLL pathogenesis and the persistence of residual disease with treatment. Each of these signaling pathways can be disrupted by the novel single small molecule BTK/PI3K/BRD4 inhibitor, SRX3305. In this study, we evaluated the preclinical efficacy of SRX3305 in CLL. We found that SRX3305 inhibits CLL cell proliferation at significantly lower doses than individual inhibitors of BTK, PI3K, and BRD4. SRX3305 proved to be not only cytostatic but also cytotoxic to CLL cells with marked induction of apoptosis. This response is in part due to irreversible binding of BTK, inhibiting its kinase function and thus significantly impairing BCR signaling. However, malignant Bcells can prioritize other signaling pathways to survive under consistent BTK inhibition. Importantly, SRX3305 also potently and irreversibly impairs phosphorylation of PI3K targets (e.g., PRAS40) and expression of BRD4 targets (e.g., MYC). Remarkably, SRX3305 sustains impressive anti-leukemic properties in ibrutinib-resistant CLL cells. SRX3305 also exhibited potent anti-tumor activity toward primary CLL cells ex vivo. The triple-inhibitor dose-dependently impaired the proliferation of stimulated primary CLL patient-derived B-cells and significantly reduced BTK phosphorylation and MYC expression in CLL cells while inducing P21 expression, indicative of cell cycle arrest. Lastly, SRX3305 significantly inhibited the proliferation of malignant B-cells from Eµ-TCL1 and Eµ-Myc/TCL1 mice ex vivo, suggesting promising therapeutic benefits in aggressive CLL and lymphomas.
CLL cells are highly dependent on diverse supportive stimuli produced by surrounding TME cells, including stromal cells in the BM and secondary lymphoid tissue niches [59]. Crosstalk between malignant and stromal cells in the TME can occur through direct cell-to-cell contact (via adhesion molecules) or indirectly by soluble factors (e.g., CXCL-12 and CXCL-13) [68]. These interactions lead to the reciprocal activation of BCR and NF-κB pathways, gene expression changes (e.g., increase in MYC and anti-apoptotic proteins), and chemotaxis, resulting in sustained growth/proliferation of leukemic cells and resistance therapeutic agents [69]. In our study, primary CLL cells co-cultured with stromal cells maintained significantly greater viability ex vivo, and this was markedly reduced in the presence of SRX3305. Bidirectional signaling networks within the TME are integral to CLL disease progression and drug response. For instance, CXCL-12/CXCR4 and CXCL-13/CXCR5 are key chemokine networks that promote CLL cell homing to protective lymphoid tissues [63][64][65]. Inhibiting the BCR signaling pathway with ibrutinib [70] or idelalisib [71] is known to impair BCR-and chemokine-mediated cell adhesion and migration in CLL. Using a trans-well system, we showed that treatment with SRX3305 significantly impairs CLL cell migration toward both CXCL-12 and CXCL-13. Preclinical studies of BET inhibitors in CLL [30,32] suggest that their efficacy may be partially attributed to interference with key CLL/TME interactions including those mediated by chemokine/cytokine networks. Interestingly, BRD4 was enriched at key CLL cell-trafficking genes (CCR7, CXCR4) in primary CLL cells [30]. Our preclinical findings demonstrate that SRX3305 can overcome TME-mediated survival signals, suggesting its efficacy within TME sanctuaries in CLL.
Selective pressure of ibrutinib monotherapy often leads to the development of drug resistance in CLL. Ibrutinib can promote the mutation of cysteine 481 of BTK required for irreversible binding of ibrutinib to BTK. We previously demonstrated that SRX3262 and SRX3305 exhibited cytotoxic effects in malignant B-cells harboring the BTK-C481S mutation [37,38]. In addition to mutations in drug-targeting proteins, several non-genetic mechanisms rendering CLL cells resistant to ibrutinib have been described and usually manifest as upregulation of alternative survival signaling pathways, such as the PI3K/AKT/ERK [67]. Here, we evaluated SRX3305 in the context of acquired resistance to prolonged ibrutinib treatment. Notably, SRX3305 was almost equipotent in ibrutinib-resistant CLL cells and their parental ibrutinib-sensitive counterpart, whereas ibrutinib was significantly less cytotoxic in ibrutinib-resistant cells. Our ibrutinib-resistant CLL cells downregulate BTK activation, suggesting an increased reliance on alternative survival pathways. The phosphorylation of PI3K target PRAS40 was inhibited in ibrutinib-resistant and parental CLL cells following SRX3305 treatment. Similarly, MYC expression was equally downregulated in ibrutinib-resistant cells with SRX3305 treatment, illustrating the potential for a triple-inhibitor to bypass the reciprocal activation of alternative survival signaling and overcome single-target drug resistance. These results suggest further evaluation of this novel chemotype in patients with CLL where responses to ibrutinib are partial or have relapse/refractory disease.
Numerous combination therapy approaches have been pursued for the treatment of CLL to combat emergence of drug resistance [14,15]. Preclinical studies have demonstrated that repressing MYC by targeting BET proteins enhances lymphoma cell vulnerability to PI3K inhibitors through the upregulation of several PI3K pathways genes and increased GSK3β phosphorylation, resulting in increased β-catenin protein abundance [72,73], suggesting that combinatory targeting of PI3K with SRX3305 would further promote MYC degradation by allowing GSK3β-dependent MYC phosphorylation [74] and stabilization of the MYC antagonist, MAD1 [75]. Our recent preclinical studies with the first-generation BTK/PI3K/BRD4 inhibitor SRX3262 in MCL demonstrated reduced phosphorylation of MYC at Ser62 and Thr58 [37], which are critical mediators of MYC protein stability and degradation in cancer [74]. Furthermore, earlier studies have reported BET inhibition to be synergistically lethal with ibrutinib in MCL and CLL models [32,76]. PI3K and BTK inhibitors have also been combined to intercept constitutive BCR pathway signaling at multiple points [77]. While combination approaches have shown promising preclinical efficacy, the increased risk of toxicity when combining multiple small-molecule-targeted agents remains an evident concern [38,78]. Individual drugs used to inhibit BTK, PI3K, and BET proteins each have unique side-effect profiles, and as such, additive side-effects from simultaneous inhibition of these pathways may occur. BTK inhibitors are associated with platelet effects and bleeding, as well as cardiovascular toxicities [79], and PI3K inhibitors carry the risk of inflammatory pneumonitis, liver toxicity, and inflammatory diarrhea [80]. BET inhibition is associated with anemia, thrombocytopenia, and neutropenia in advanced malignancies [81,82]. Combining multiple agents/drugs presents challenges in tailoring therapeutic regimens based on each drug's unique pharmacokinetic profile (adsorption, distribution, metabolism, and elimination) and additive adverse toxicities [83,84]. A multitarget inhibitor such as SRX3305 provides the advantage of multi-axis inhibition with a single compound that may result in a better toxicity profile and reduced pill burden, which is a major issue impacting the adherence of patients to multi-drug therapeutic regimens. Future studies evaluating the pharmacokinetic and pharmacodynamic effects of this novel class of inhibitors in vivo are needed to delineate the associated toxicities and translational potential of such promising TP-scaffold multi-action inhibitors for the treatment of B-cell malignancies.

Cell Lines, Primary Samples, and Inhibitors
Malignant B-cell lines (MEC-1, MEC-2, HG-3, OCI-LY3) were purchased from DSMZ (Braunschweig, Germany), while SU-DHL-6 cells were purchased from ATCC (Gaithersburg, MD, USA). The OSU-CLL cell line [85] was provided by the Human Genetics Sample Bank of The Ohio State University (OSU; Columbus, OH, USA). The murine 9-15c stromal cell line [86] was obtained from RIKEN (Ibaraki, Japan). To generate ibrutinib-resistant cells, HG-3 cells were treated with progressively increasing ibrutinib concentrations (up to 25 µM) over the course of 3 months, as further detailed in the Supplementary Materials. CLL patient samples (peripheral blood mononuclear cells; PBMCs) were obtained from the Leukemia Tissue Bank (OSU) in accordance with the Declaration of Helsinki following informed consent and under a protocol approved by the Institutional Review Board at OSU. In brief, PBMCs from CLL patients were isolated from whole blood through ficoll density gradient centrifugation. Prior to use in experiments, CLL patient-derived samples were confirmed to contain more than 90% CD5 + /CD19 + cells by flow cytometric analysis on a NovoCyte 2060R flow cytometer (ACEA Biosciences Inc., San Diego, CA, USA).
Lymphocytes isolated from Eµ-TCL1 and Eµ-Myc/TCL1 mouse spleens were obtained from the Animal Research Facility of the University of Nebraska Medical Center (UNMC), adhering to institutional animal care guidelines. Spleens were harvested from terminally ill mice (i.e., moribund at humane endpoints), and the percentage of malignant B-cells was confirmed via flow cytometry to exceed 90% CD5 + /CD19 + prior to experimental use. Fluorochrome-conjugated CD19 and CD5 antibodies were ordered from BD Biosciences (Franklin Lakes, NJ, USA).

BCR Pathway Activation
To induce B-cell receptor crosslinking, OSU-CLL and MEC-1 were treated with 10 µg/mL of goat F(ab')2 anti-human IgM (Jackson ImmunoResearch, West Grove, PA, USA) for the final 15 min of treatment. Cells were then harvested and lysed for immunoblot analyses.

Inhibitor Washout Assay
OSU-CLL and MEC-1 cells were treated with indicated concentrations of inhibitors or vehicle for 1 h, washed three times with PBS, and incubated in complete culture medium for 3 h. For continuous treatment, the inhibitors or vehicle control remained for the entire treatment duration (4 h). In the final 15 min of treatment, cells were treated with anti-IgM to induce BCR crosslinking as described above and lysed for immunoblot analyses.

Ex Vivo Stimulation of Primary Malignant B-Cells
Primary CLL cells were cultured with 3.2 µM CpG 2006 oligodeoxynucleotides (Integrated DNA Technologies) to induce proliferation for the duration of treatment. For murine samples, 1× phorbol 12-myristate 13-acetate (PMA)/ionomycin cell stimulation cocktail (eBioscience, San Diego, CA, USA) was added during treatments.

Immunoblot Analyses
Cells were treated with indicated concentrations of inhibitors or vehicle control for 4 h under different stimuli. Thereafter, lysates were prepared, analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and probed for select proteins. See Supplementary Methods for details.

Migration Assay
Following 1 h pre-treatment with DMSO, SRX3305, or ibrutinib, MEC-1 cells were placed onto 5-micron trans-well inserts (Corning, Tewksbury, MA, USA) resting in wells containing 200 ng/mL CXCL-12 or 1000 ng/mL CXCL-13 (PeproTech, Cranbury, NJ, USA). No chemokine control wells were included for each treatment condition. After incubating for 6 h, trans-well inserts were carefully removed, and the number of cells that migrated through the insert towards the chemokines were counted by flow cytometry. Data were analyzed using NovoExpress software (ACEA Biosciences Inc., San Diego, CA, USA).

Statistical Analyses
Differences in cell viability and apoptosis between conditions of interest were assessed using ANOVA models. Dunnett's test was used to compare conditions and dose levels with vehicle control. A test of linear trend for SRX3305 dosing was performed by employing orthogonal polynomial contrast coefficients. Log-transformations of the data were applied, when necessary, before modeling to stabilize variances specifically for data analyzed as raw absorption. Percent proliferation data or percent viable data were analyzed without transformation. Data are represented as mean ± SEM. Analyses were conducted using SAS/STAT software, version 9.4 of the SAS System for Windows (SAS Institute Inc., Cary, NC, USA). p-values < 0.05 were considered statistically significant.

Conclusions
The novel BTK/PI3K/BRD4 inhibitor, SRX3305, demonstrates marked anti-tumor properties in preclinical models of CLL. Importantly, the pro-survival, proliferative, therapyresistant, and activation effects promoted by TME sanctuaries were abrogated by SRX3305, which furthermore blocked CLL cell chemotaxis. Additionally, SRX3305 sustained impressive anti-tumor effects in ibrutinib-resistant CLL cells and effectively attenuated alternative/downstream survival signaling pathways. Together, these findings provide strong rationale for the clinical development of this distinct triple-action inhibitor for treating B-cell lymphoproliferative diseases, especially in the relapse/refractory setting.   [36] and are not publicly available. All other data will be made available from the authors upon reasonable request.