Next Article in Journal
Thirty Years’ History since the Discovery of Pax6: From Central Nervous System Development to Neurodevelopmental Disorders
Next Article in Special Issue
Curcumin Induces Apoptosis of Chemoresistant Lung Cancer Cells via ROS-Regulated p38 MAPK Phosphorylation
Previous Article in Journal
MicroRNA-365a/b-3p as a Potential Biomarker for Hypertrophic Scars
 
 
Article

APTC-C-SA01: A Novel Bacteriophage Cocktail Targeting Staphylococcus aureus and MRSA Biofilms

1
Department of Surgery-Otolaryngology Head and Neck Surgery, Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, Woodville, SA 5011, Australia
2
Adelaide Medical School, The University of Adelaide, Adelaide, SA 5000, Australia
3
School of Biological Sciences, The University of Adelaide, Adelaide, SA 5000, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Maria José Umbelino Ferreira
Int. J. Mol. Sci. 2022, 23(11), 6116; https://doi.org/10.3390/ijms23116116
Received: 26 April 2022 / Revised: 25 May 2022 / Accepted: 26 May 2022 / Published: 30 May 2022
The high infection and mortality rate of methicillin-resistant Staphylococcus aureus (MRSA) necessitates the urgent development of new treatment strategies. Bacteriophages (phages) have several advantages compared to antibiotics for the treatment of multi-drug-resistant bacterial infections, and thus provide a promising alternative to antibiotics. Here, S. aureus phages were isolated from patients and environmental sources. Phages were characterized for stability, morphology and genomic sequence and their bactericidal activity against the biofilm form of methicillin-susceptible Staphylococcus aureus (MSSA) and MRSA was investigated. Four S. aureus phages were isolated and tested against 51 MSSA and MRSA clinical isolates and reference strains. The phages had a broad host range of 82–94% individually and of >98% when combined and could significantly reduce the viability of S. aureus biofilms. The phages had a latent period of ≤20 min and burst size of >11 plaque forming units (PFU)/infected cell. Transmission electron microscopy (TEM) identified phages belonging to the family of Myoviridae. Genomic sequencing indicated the lytic nature of all four phages, with no identified resistance or virulence genes. The 4 phages showed a high complementarity with 49/51 strains (96%) sensitive to at least 2/4 phages tested. Furthermore, the frequency of bacteriophage insensitive mutant (BIM) generation was lower when the phages were combined into the phage cocktail APTC-C-SA01 than for bacteria exposed to each of the phages alone. In conclusion, APTC-C-SA01, containing four lytic S. aureus phages has the potential for further development as a treatment against MSSA and MRSA infections. View Full-Text
Keywords: S. aureus; antimicrobial; bacteriophage; biofilm; phage cocktail S. aureus; antimicrobial; bacteriophage; biofilm; phage cocktail
Show Figures

Figure 1

MDPI and ACS Style

Liu, S.; Hon, K.; Bouras, G.S.; Psaltis, A.J.; Shearwin, K.; Wormald, P.-J.; Vreugde, S. APTC-C-SA01: A Novel Bacteriophage Cocktail Targeting Staphylococcus aureus and MRSA Biofilms. Int. J. Mol. Sci. 2022, 23, 6116. https://doi.org/10.3390/ijms23116116

AMA Style

Liu S, Hon K, Bouras GS, Psaltis AJ, Shearwin K, Wormald P-J, Vreugde S. APTC-C-SA01: A Novel Bacteriophage Cocktail Targeting Staphylococcus aureus and MRSA Biofilms. International Journal of Molecular Sciences. 2022; 23(11):6116. https://doi.org/10.3390/ijms23116116

Chicago/Turabian Style

Liu, Sha, Karen Hon, George Spyro Bouras, Alkis James Psaltis, Keith Shearwin, Peter-John Wormald, and Sarah Vreugde. 2022. "APTC-C-SA01: A Novel Bacteriophage Cocktail Targeting Staphylococcus aureus and MRSA Biofilms" International Journal of Molecular Sciences 23, no. 11: 6116. https://doi.org/10.3390/ijms23116116

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop