Correction: Wu, W., et al. Tip60 Phosphorylation at Ser 99 Is Essential for Autophagy Induction in Bombyx mori. Int. J. Mol. Sci. 2020, 21, 6893
by
Wenmei Wu
1,2, 
Kang Li
3,
Haigang Zhao
3,
Xianying Xu
1,
Jing Xu
1,
Man Luo
1,
Yang Xiao
4 and
Ling Tian
1,* 1
Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding/Guangdong Provincial Sericulture and Mulberry Engineering Research Center, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
2
Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China
3
Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China
4
The Sericultural and Agri-Food Research Institute of the Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(4), 1751; https://doi.org/10.3390/ijms22041751
Submission received: 30 December 2020
/
Accepted: 6 January 2021
/
Published: 10 February 2021
(This article belongs to the Special Issue Molecular Ecology, Physiology and Biochemistry of Insects 2.0)
The author wishes to make the following correction to this paper [1]. Due to an error involving the immunofluorescent staining for BmAtg8 after starvation treatment in Figure 4E, it should be replaced with the correct new figure (Figure 1). Due to a change in the statistical analysis between the three groups as well as the replacements of the merged and magnified pictures for BmTip60WT in Figure 6D, the corrected figure is shown below (Figure 2).
The authors would like to apologize for any inconvenience caused to the readers by these changes.
Conflicts of Interest
The authors declare no conflict of interest.
Reference
- Wu, W.; Li, K.; Zhao, H.; Xu, X.; Xu, J.; Luo, M.; Xiao, Y.; Tian, L. Tip60 Phosphorylation at Ser 99 Is Essential for Autophagy Induction in Bombyx mori. Int. J. Mol. Sci. 2020, 21, 6893. [Google Scholar] [CrossRef] [PubMed]
Figure 1.
Starvation but not 20-hydroxyecdysone (20E) promotes BmTip60 in addition to autophagy in B. mori fat body. (A) mRNA levels of BmE75a after 20E treatment for 24 h. (B) Protein levels of BmSqstm1, BmTip60, and BmAtg8 after 20E treatment. Two repeats are shown here. (C) LysoTracker red staining and BmAtg8 immunofluorescent staining after 20E treatment. Arrow: BmAtg8 puncta. Scale bar: 10 micron. (D) Protein levels of BmSqstm1, BmTip60, p-AMPKα (T172), and BmAtg8 after starvation for 24 h. Two repeats are shown here. (E) LysoTracker red staining and BmAtg8 immunofluorescent staining after starvation. Arrow: Atg8 puncta in the cytoplasm. Scale bar: 10 micron. (E’) Quantification of BmAtg8 puncta in (E). (F) Immunofluorescent staining of BmTip60 after starvation. White solid box: magnified field. White dashed box: original field. Scale bar: 10 micron. (F’) Quantification of BmTip60 density in (F). One-way analysis of variance was performed by SPSS in the whole figure, * p < 0.05; ** p < 0.01; *** p < 0.001, n = 200 cells.
Figure 1.
Starvation but not 20-hydroxyecdysone (20E) promotes BmTip60 in addition to autophagy in B. mori fat body. (A) mRNA levels of BmE75a after 20E treatment for 24 h. (B) Protein levels of BmSqstm1, BmTip60, and BmAtg8 after 20E treatment. Two repeats are shown here. (C) LysoTracker red staining and BmAtg8 immunofluorescent staining after 20E treatment. Arrow: BmAtg8 puncta. Scale bar: 10 micron. (D) Protein levels of BmSqstm1, BmTip60, p-AMPKα (T172), and BmAtg8 after starvation for 24 h. Two repeats are shown here. (E) LysoTracker red staining and BmAtg8 immunofluorescent staining after starvation. Arrow: Atg8 puncta in the cytoplasm. Scale bar: 10 micron. (E’) Quantification of BmAtg8 puncta in (E). (F) Immunofluorescent staining of BmTip60 after starvation. White solid box: magnified field. White dashed box: original field. Scale bar: 10 micron. (F’) Quantification of BmTip60 density in (F). One-way analysis of variance was performed by SPSS in the whole figure, * p < 0.05; ** p < 0.01; *** p < 0.001, n = 200 cells.
Figure 2.
Phosphorylation at Ser99 is required for BmTip60-mediated autophagy. (A) Amino acid sequences of Tip60 homologs in B. mori and H. sapiens are aligned by DNAMAN software. Dark blue: conserved amino acid sequence, light blue: nonconservative amino acid sequence. Red box: the conserved phosphorylation site. (B) Phosphorylation level of immunoprecipitated BmTip60 after overexpression of BmTip60-V5, BmTip6099SA-V5, and BmTip6099SD-V5 in BmN cells for 48 h, respectively. WT: wild-type; 99SA: Serine99 mutated to Alanine99; 99SD: Serine99 mutated to Aspartic99. (C) Protein levels of BmSqstm1, BmTip60, and BmAtg8 after overexpression of BmTip60-V5, BmTip6099SA-V5, or BmTip6099SD-V5 in BmN cells for 48 h. (D) Observation of EGFP-BmAtg8 and immunostained BmTip60-V5/BmTip6099SA-V5/BmTip6099SD-V5 under nutrient-rich conditions in BmN cells. Scale bar: 10 micron. White solid box: magnified field. White dashed box: original field. (D’) Quantification of EGFP-BmAtg8 puncta in (D). One-way analysis of variance was performed by SPSS in the whole figure; different lowercase letters mean p < 0.05, n = 50 cells.
Figure 2.
Phosphorylation at Ser99 is required for BmTip60-mediated autophagy. (A) Amino acid sequences of Tip60 homologs in B. mori and H. sapiens are aligned by DNAMAN software. Dark blue: conserved amino acid sequence, light blue: nonconservative amino acid sequence. Red box: the conserved phosphorylation site. (B) Phosphorylation level of immunoprecipitated BmTip60 after overexpression of BmTip60-V5, BmTip6099SA-V5, and BmTip6099SD-V5 in BmN cells for 48 h, respectively. WT: wild-type; 99SA: Serine99 mutated to Alanine99; 99SD: Serine99 mutated to Aspartic99. (C) Protein levels of BmSqstm1, BmTip60, and BmAtg8 after overexpression of BmTip60-V5, BmTip6099SA-V5, or BmTip6099SD-V5 in BmN cells for 48 h. (D) Observation of EGFP-BmAtg8 and immunostained BmTip60-V5/BmTip6099SA-V5/BmTip6099SD-V5 under nutrient-rich conditions in BmN cells. Scale bar: 10 micron. White solid box: magnified field. White dashed box: original field. (D’) Quantification of EGFP-BmAtg8 puncta in (D). One-way analysis of variance was performed by SPSS in the whole figure; different lowercase letters mean p < 0.05, n = 50 cells.
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Wu, W.; Li, K.; Zhao, H.; Xu, X.; Xu, J.; Luo, M.; Xiao, Y.; Tian, L. Correction: Wu, W., et al. Tip60 Phosphorylation at Ser 99 Is Essential for Autophagy Induction in Bombyx mori. Int. J. Mol. Sci. 2020, 21, 6893. Int. J. Mol. Sci. 2021, 22, 1751. https://doi.org/10.3390/ijms22041751
AMA Style
Wu W, Li K, Zhao H, Xu X, Xu J, Luo M, Xiao Y, Tian L. Correction: Wu, W., et al. Tip60 Phosphorylation at Ser 99 Is Essential for Autophagy Induction in Bombyx mori. Int. J. Mol. Sci. 2020, 21, 6893. International Journal of Molecular Sciences. 2021; 22(4):1751. https://doi.org/10.3390/ijms22041751
Chicago/Turabian StyleWu, Wenmei, Kang Li, Haigang Zhao, Xianying Xu, Jing Xu, Man Luo, Yang Xiao, and Ling Tian. 2021. "Correction: Wu, W., et al. Tip60 Phosphorylation at Ser 99 Is Essential for Autophagy Induction in Bombyx mori. Int. J. Mol. Sci. 2020, 21, 6893" International Journal of Molecular Sciences 22, no. 4: 1751. https://doi.org/10.3390/ijms22041751
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