An Engineered Thermostable Laccase with Great Ability to Decolorize and Detoxify Malachite Green

Laccases can catalyze the remediation of hazardous synthetic dyes in an eco-friendly manner, and thermostable laccases are advantageous to treat high-temperature dyeing wastewater. A novel laccase from Geothermobacter hydrogeniphilus (Ghlac) was cloned and expressed in Escherichia coli. Ghlac containing 263 residues was characterized as a functional laccase of the DUF152 family. By structural and biochemical analyses, the conserved residues H78, C119, and H136 were identified to bind with one copper atom to fulfill the laccase activity. In order to make it more suitable for industrial use, Ghlac variant Mut2 with enhanced thermostability was designed. The half-lives of Mut2 at 50 °C and 60 °C were 80.6 h and 9.8 h, respectively. Mut2 was stable at pH values ranging from 4.0 to 8.0 and showed a high tolerance for organic solvents such as ethanol, acetone, and dimethyl sulfoxide. In addition, Mut2 decolorized approximately 100% of 100 mg/L of malachite green dye in 3 h at 70 °C. Furthermore, Mut2 eliminated the toxicity of malachite green to bacteria and Zea mays. In summary, the thermostable laccase Ghlac Mut2 could effectively decolorize and detoxify malachite green at high temperatures, showing great potential to remediate the dyeing wastewater.


Introduction
Laccases (benzenediol: oxygen oxidoreductase, EC 1.10.3.2), a member of polyphenol oxidases, can oxidize a wide range of phenolic and nonphenolic compounds [1]. Laccases typically contain four copper atoms in their active center [1]. Type 1 copper, which exhibits strong electronic absorbance at 610 nm, can abstract one electron from the substrate. During the subsequent electron transferring, oxygen is reduced to water at the trinuclear center formed by Type 2 and 3 copper [2]. Thanks to their broad substrate specificity and the green reaction only requiring oxygen and releasing water as the sole by-product, laccases have been applied for industrial use such as delignification to improve biomass saccharification [3], biobleaching [4], degradation of environmental pollutants [5], and decolorization and detoxification of dyes [6,7].
Laccases are widely distributed in fungi, plants, insects, and bacteria. Bacterial laccases exhibit rather low redox potential about 400 mV as compared with fungal laccases with higher redox potential between 470 and 810 mV [8]. Because of the high redox potential, the laccases from fungi have been the focus of research, and their applications have been extensively exploited [9]. However, the long production cycle, poor thermostability, and low tolerance for the alkaline condition hinder the practical application of fungal The open reading frame of Ghlac encoding an uncharacterized protein containing the consensus sequences of DUF152 laccases was found in the thermophile G. hydrogeniphilus. Ghlac contains 263 residues with a theoretical molecular weight of 29.0 kDa. Multiple sequence alignment showed that Ghlac shares 22.6%, 30.2%, and 34.0% identities to LaclK, RL5, and Tfu1114, respectively ( Figure 1A). The putative copper binding sites (N41, H78, C119, and H136) were conserved in Ghlac [22]. The structure model indicated that Ghlac has a similar structural fold to the DUF152 member GsYlmD ( Figure 1B).
The half-life (t 1/2 ) of Ghlac wild type (WT) at 50 • C was less than 24 h ( Figure 3D), which could hardly satisfy the requirement for industrial application. In order to improve the thermostability, Ghlac variants Mut1, Mut2, and Mut3 were designed by PROSS and characterized ( Figure S1) [26]. Among these variants, Mut2 showed increased ther-

Effects of pH and Temperature on the Activity and Stability of Ghlac
The optimal pH for Ghlac WT and Mut2 against ABTS was 4.0 ( Figure 3A), which is in accordance with the acidic pH preference of fungal and bacterial laccases [9,19]. Ghlac lost more than 60% of its original activity after incubation at pH 3.0 for 6 h, while Mut2 retained more than 95% of the original activity over the pH range of 4.0 to 8.0 ( Figure 3C), similar to the characterized DUF152 laccases [22][23][24]. The bacterial laccases from B. stratosphericus, γ-proteobacterium, and a marine microbial metagenomic library showed high tolerance for alkaline conditions, which is an advantageous property of laccases from bacteria [27][28][29]. By contrast, most of the laccases from fungi are unstable under alkaline conditions [5,30].
Ghlac WT and Mut2 showed their maximal activity at 60 • C ( Figure 3B). Their thermostability was tested to evaluate the potential for industrial application. Mut2 retained 100% of its original activity after incubation at 50 • C for 6 h, whereas Ghlac WT lost 20% of its activity. Furthermore, t 1/2 of Ghlac was calculated ( Figure 3D). t 1/2 of Mut2 at 50 • C was 80.6 h, 3.7 times longer than that of WT. t 1/2 of Mut2 at 60 • C was increased to 9.6 h, compared with that of WT. Additionally, T m of Mut2 was 6.9 • C higher than that of WT ( Figure 4A), which is consistent with the results of t 1/2 measurements. Most of the fungal laccases could not retain 50% of the original activity after incubation at 60 • C for 6 h [30]. rLac from Klebsiella pneumoniae only retained 60% and 50% of the activity for 5 h at 50 • C and 60 • C, respectively [31]. The spore-coat laccase FNTL from Bacillus sp. lost 80% of the activity at 60 • C for 5 h [19]. Therefore, Mut2 is highly thermostable. Based on the principle of PROSS and the structural mapping of the mutated residues of Mut2 ( Figures S1 and S3), the introduced mutations increased the surface polarity (such as S58E and G197E) and rigidified the flexible elements (such as A35P and V68P), significantly enhancing the thermostability of Mut2 [26,32,33].
of the activity at 60 °C for 5 h [19]. Therefore, Mut2 is highly thermostable. Based on the principle of PROSS and the structural mapping of the mutated residues of Mut2 (Figures S1 and S3), the introduced mutations increased the surface polarity (such as S58E and G197E) and rigidified the flexible elements (such as A35P and V68P), significantly enhancing the thermostability of Mut2 [26,32,33].  The determined K m and k cat of Mut2 were comparable to those of Ghlac WT ( Figure 2A). Mut2 was able to oxidize ABTS, 2,6-dimethoxyphenol (DMP), and guaiacol, and the substrate preference decreased in the order of DMP > guaiacol > ABTS ( Figure 2B). However, syringaldazine (SGZ) could not be catalyzed by Mut2.
Therefore, Mut2 is a functional polyphenol oxidase of the DUF152 family, although it was cloned from an anaerobe. Berini et al. also reported a functional laccase from the anaerobe Geobacter metallireducens and discussed the possible mechanism using small amounts of oxygen in the environment or other chemical (such as N 2 O) as the electron acceptor [34]. The physiological role of Ghlac in G. hydrogeniphilus needs to be further studied by thorough in vivo experiments. Overall, the excellent thermostability makes Mut2 a potential catalyst for industrial application.

Identification of the Putative Copper Binding Site
The typical laccase contains four copper atoms, and the copper is required to transfer electrons during catalysis [5]. When the concentration of Cu 2+ increased, the activity of Mut2 also increased accordingly, indicating that Mut2 is Cu 2+ -dependent ( Figure 4C). Moreover, the Thermofluor assay showed that T m of Mut2 was increased from 56.4 • C to 60.9 • C in the presence of Cu 2+ ( Figure 4A), implying that Cu 2+ helps to stabilize Mut2 by interaction [35]. However, the UV/visible spectra of Mut2 ( Figure 4B), Tfu1114, and LaclK lacked the absorption peak around 610 nm, a characteristic of the typical laccases [23,24], showing that DUF152 laccases might interact with the copper in a different way. Furthermore, ICP-MS detected 1.1 ± 0.1 mol/mol of copper in Mut2 ( Figure 4F), which corresponds to the copper content of Tfu1114 and LaclK, but differs from that of RL5 (4.0 ± 0.2 mol/mol) [22][23][24]. Among the 12 identified residues forming the putative copper binding sites in RL5 [22], only N41, H78, C119, and H136 are conserved in the characterized DUF152 laccases ( Figure 1A). Thus, could the four conserved residues constitute the putative copper binding site of Mut2? Structural analysis showed that N41 of Mut2 is far from the cluster formed by H78, C119, and H136 ( Figure 1B), and the cluster is bound with one Zn 2+ in the crystal structure of GsYlmd ( Figure 4D). Hence, variants H78A, C119A, H136A, and 3A were constructed to confirm whether the putative binding site is formed by H78, C119, and H136. Variants H78A, C119A, and H136A showed significantly decreased laccase activity, while variant 3A almost abolished its activity ( Figure 4E). Meanwhile, no significant difference was detected between the T m values of Mut2 and the variant 3A ( Figure 4A), indicating that the mutation of H78, C119, and H136 did not change the native structure of Mut2. Furthermore, no copper was detected in the variant 3A ( Figure 4F). Correspondingly, the addition of Cu 2+ did not increase T m of 3A ( Figure 4A).
Taken together, the conserved residues H78, C119, and H136 constitute the putative copper binding site of Mut2 and bind with one copper atom to exert the laccase activity. However, the substrate binding site and the catalytic mechanism of Mut2 are yet to be elucidated by crystal structures of Ghlac complexed with substrates.

Effects of Metal Ions and Organic Solvents on the Activity of Mut2
As shown in Figure 5A, Mut2 maintained over 82% residual activity in the presence of 10 mM Na + , Mg 2+ , Ca 2+ , Mn 2+ , and Ni 2+ . Although the activity of Mut2 was not strongly affected by 1 mM Zn 2+ and Ba 2+ , it was dramatically reduced to 47% and 0, respectively, when the metal ions concentration increased to 10 mM. This inhibitory effects of high-concentration Zn 2+ or Ba 2+ were generally reported in laccases such as BaCotA, PvL, SN4LAC, and MSKLAC [27,[36][37][38]. As the essential factor for the activity of Mut2, 10 mM Cu 2+ did not show any inhibitory effect on Mut2 ( Figure 4C). With the addition of 1 mM chelating agent EDTA, Mut2 completely lost its activity, indicating the importance of Cu 2+ during catalysis. The effects of organic solvents on the activity of Mut2 were also assessed ( Figure 5B). Mut2 retained more than 90% and 80% of its original activity in the presence of 10% and 20% methanol, ethanol, acetone, and isopropanol, respectively. In the presence of 10% dimethyl sulfoxide, the activity of Mut2 was boosted to 120%. This kind of boosted laccase activity was also reported in laccases from Cerrena sp. RSD1, T. versicolor, and a marine metagenomic library [39,40], whereas laccases from B. stratosphericus, Ganoderma lucidum, and Sporothrix carnis were strongly inhibited by 10% dimethyl sulfoxide [27,41,42]. Moreover, Mut2 could retain 90% of its activity in the presence of 10% formaldehyde, which is a powerful protein denaturant, and could strongly inhibit the laccase from B. amyloliquefaciens [43]. These results indicated that Mut2 is tolerant to the organic solvents.
Overall, Mut2 is quite resistant to common environmental pollutants, including metal ions and organic solvents.

MG Decolorization Catalyzed by Mut2
The dark color and strong toxicity of MG discharged from the dyeing industry severely threaten the environment and human health [13]. However, conventional physical The effects of organic solvents on the activity of Mut2 were also assessed ( Figure 5B). Mut2 retained more than 90% and 80% of its original activity in the presence of 10% and 20% methanol, ethanol, acetone, and isopropanol, respectively. In the presence of 10% dimethyl sulfoxide, the activity of Mut2 was boosted to 120%. This kind of boosted laccase activity was also reported in laccases from Cerrena sp. RSD1, T. versicolor, and a marine metagenomic library [39,40], whereas laccases from B. stratosphericus, Ganoderma lucidum, and Sporothrix carnis were strongly inhibited by 10% dimethyl sulfoxide [27,41,42]. Moreover, Mut2 could retain 90% of its activity in the presence of 10% formaldehyde, which is a powerful protein denaturant, and could strongly inhibit the laccase from B. amyloliquefaciens [43]. These results indicated that Mut2 is tolerant to the organic solvents.
Overall, Mut2 is quite resistant to common environmental pollutants, including metal ions and organic solvents.

MG Decolorization Catalyzed by Mut2
The dark color and strong toxicity of MG discharged from the dyeing industry severely threaten the environment and human health [13]. However, conventional physical and chemical treatment processes usually produce secondary sludge and hazardous byproducts, resulting in serious environmental pollution [44,45]. Laccase, as a green catalyst, has drawn increasing attention, being a potential alternative to tackle dye pollution in an environmentally friendly manner [27,45,46].
Thanks to the reasonably good thermostability of Mut2 and the high temperature of the dyeing wastewater [47,48], the capability of MG decolorization by Mut2 was investigated at high temperatures. Mut2 alone decolorized only 10% of 100 mg/L of MG at 60 • C in 2 h. Redox mediators have been used to improve dye decolorization [11]. Among the tested mediators, ABTS was the most effective one ( Figure 6A). With the help of 0.1 mM ABTS, the MG decolorization rate by Mut2 was improved to 90% ( Figure 6B). The improvement by ABTS is in accordance with the study of laccases BaCotA and rLAC [27,46]. The optimal pH and temperature for MG decolorization were 3.5-4.0 and 70 • C, respectively ( Figure 6C,D). The decolorization rate was more than 90% in the temperature range of 60-75 • C, and the highest rate of 98% was reached at 70 • C in 2 h. Decolorization was almost completed by Mut2 in 3 h, whereas only 78% of MG was decolorized by Ghlac WT, showing the advantage of thermostable laccases to treat high-temperature dyeing wastewater ( Figures 6E and S4). The disappearance of the absorption peak at 617 nm indicated the destruction of the conjugated chromophore structure of MG, which showed that Ghlac Mut2 may decolorize MG by the oxidation and cleavage of the chromophore of MG in a similar way to the typical laccases [15,16]. showed that Ghlac Mut2 may decolorize MG by the oxidation and cleavage of the chromophore of MG in a similar way to the typical laccases [15,16]. The textile effluent usually has a relatively high temperature, above 50 °C and even up to 70-80 °C [20,47], and a high temperature would benefit the decolorization velocity [21]. In order to avoid the extra cooling process and maximize the decolorization rate, more and more studies focus on MG decolorization by laccases at high temperatures ( Table 1). LaclK of the DUF152 family was highly thermostable, but its ability to decolorize MG was poor [24]. Although BaCotA, CueO-p, rLAC, and rLac could effectively decolorize MG, the relatively low thermostability would result in incomplete decolori- The textile effluent usually has a relatively high temperature, above 50 • C and even up to 70-80 • C [20,47], and a high temperature would benefit the decolorization velocity [21]. In order to avoid the extra cooling process and maximize the decolorization rate, more and more studies focus on MG decolorization by laccases at high temperatures ( Table 1).
LaclK of the DUF152 family was highly thermostable, but its ability to decolorize MG was poor [24]. Although BaCotA, CueO-p, rLAC, and rLac could effectively decolorize MG, the relatively low thermostability would result in incomplete decolorization, even with an extended incubation time [27,31,46,49]. Thus, the remaining MG would still threaten public health. Compared with these laccases, the identified Mut2 herein is superior in both thermostability and the completeness of decolorization and, consequently, more suitable for industrial application.

MG Detoxification Catalyzed by Mut2
The toxicity of MG before and after Mut2 treatment was estimated to evaluate whether Mut2 could detoxify MG.
As Figure 7A,B illustrated, Escherichia coli and B. subtilis did not grow in LB containing untreated MG, indicating that MG is toxic to bacteria. As expected, in both the control group and the treated MG group, bacteria grew in almost the same manner, indicating that the toxicity of MG to bacteria was eliminated by Mut2. A phytotoxicity test of MG before and after Mut2 treatment was also carried out with Zea mays by recording seed germination and the elongation of radical and plumule ( Figure 7C-E). Compared with the control group, MG inhibited the germination of Z. mays seeds by 55%, and the length of radical and plumule was only about 38% of that in the control group. In the treated MG group, the growth of Z. mays seeds showed no difference from that in the control group ( Figure 7C-E). However, MG treated by the laccase LacA from Cerrena sp. still inhibited the root elongation of Nicotiana tabacum and Lactuca sativa [15]. These results revealed that Mut2 could eliminate the toxicity of MG to bacteria and Z. mays.

Cloning, Expression, and Purification of Ghlac
The gene encoding Ghlac WT (NCBI accession No.: ORJ60343.1, https://www.ncbi.nlm.nih.gov/protein/ORJ60343.1, accessed on 15 June 2019) was codon-optimized, synthesized, and cloned in the pET-28a (+) vector using the Nco I and Xho I restriction sites. The obtained recombinant vector pET28a-Ghlac-WT was transformed into E. coli BL21 (DE3). The cells containing the recombinant vector were grown in Luria-Bertani (LB) medium supplemented with 25 mg/L of kanamycin. When OD600 reached 0.6, 0.5 mM IPTG and 0.5 mM CuSO4 were added to induce the expression of Ghlac, and then the cells continued to grow at 16 °C for 16 h. The cells were collected by centrifugation at 4000× g for 30 min and homogenized using a JN-Mini homogenizer (JNBio, Guangzhou, China). The recombinant Ghlac in the supernatant was purified using Ni-NTA resin according to the reported method [50]. The purified Ghlac in 20 mM phosphate buffer (pH 7.

Cloning, Expression, and Purification of Ghlac
The gene encoding Ghlac WT (NCBI accession No.: ORJ60343.1, https://www.ncbi. nlm.nih.gov/protein/ORJ60343.1, accessed on 15 June 2019) was codon-optimized, synthesized, and cloned in the pET-28a (+) vector using the Nco I and Xho I restriction sites. The obtained recombinant vector pET28a-Ghlac-WT was transformed into E. coli BL21 (DE3). The cells containing the recombinant vector were grown in Luria-Bertani (LB) medium supplemented with 25 mg/L of kanamycin. When OD 600 reached 0.6, 0.5 mM IPTG and 0.5 mM CuSO 4 were added to induce the expression of Ghlac, and then the cells continued to grow at 16 • C for 16 h. The cells were collected by centrifugation at 4000× g for 30 min and homogenized using a JN-Mini homogenizer (JNBio, Guangzhou, China). The recombinant Ghlac in the supernatant was purified using Ni-NTA resin according to the reported method [50]. The purified Ghlac in 20 mM phosphate buffer (pH 7.4) was stored at −80 • C. The purity and molecular mass of Ghlac were assessed by SDS-PAGE. The UV/visible absorption spectrum of Ghlac was scanned in the range of 200-800 nm using a SpectraMax M2e Microplate Reader (Molecular Devices, Sunnyvale, CA, USA). The copper content of Ghlac was analyzed with an iCAP Qc inductively coupled plasma mass spectrometry (ICP-MS) (ThermoFisher Scientific, Waltham, MA, USA) [22].

Mutation Design Using PROSS and Site-Directed Mutagenesis
Goldenzweig et al. developed an automated structure-and sequence-based algorithm, the Protein Repair One Stop Shop (PROSS) webserver, to design protein variants with enhanced stability requiring minimal experimental testing (accessed on 18 May 2020) [26].
Ghlac sequence was submitted to PROSS with N41, H78, C119, and H136 constrained to improve the thermostability. The designed variants with 17, 25, and 31 mutated residues (referred to as Ghlac Mut1, Mut2, and Mut3, respectively; Figure S1) were chosen to test according to the manual of PROSS.
The variants H78A, C119A, and H136A, as well as the combination of H78A, C119A, and H136A (referred to as 3A), of Ghlac Mut2 were constructed using the one step sitedirected mutagenesis method. Briefly, the primers with the desired mutation were designed and synthesized (Table S1). PCR was performed with the plasmid pET28a-Ghlac-Mut2 as the template. The PCR products were digested with Dpn I at 37 • C for 4 h and transformed into DH5α competent cells. The variants were confirmed by sequencing, expressed, and purified according to the method described in Section 3.2.

Enzyme Activity Assay
The activity of Ghlac was determined with ABTS (ε 420 = 38,000 M −1 cm −1 ) as the substrate. The reaction system contained appropriately diluted purified Ghlac, 1 mM ABTS, 1 mM CuSO 4 , and 20 mM acetic acid-sodium acetate buffer (pH 4.0). The reaction samples were incubated at 50 • C for 10 min and then put on ice for 5 min to terminate the reaction. The absorbance at 420 nm was measured. The laccase activity against 1 mM DMP (ε 468 = 35,640 M −1 cm −1 ), 1 mM guaiacol (ε 470 = 26,600 M −1 cm −1 ), and 25 µM SGZ (ε 530 = 64,000 M −1 cm −1 ) was also determined. One unit of enzyme activity (1 U) was defined as the amount of enzyme required to oxidize 1 µmol of substrate per minute at 50 • C. The kinetic parameters (K m and k cat ) of Ghlac toward ABTS were determined at 60 • C according to the standard laccase assay method.

Effects of Temperature and pH on the Laccase Activity and Stability
The effect of pH on the laccase activity was determined using 20 mM acetic acidsodium acetate (pH 3.0-6.0) and 20 mM Tris-HCl (pH 7.0-8.0). To evaluate its pH stability, Ghlac was incubated in different pH buffers (3.0-8.0) at 4 • C for 6 h, and the residual activity was measured according to the standard laccase assay method. The effect of temperature on the laccase activity was analyzed in 20 mM acetic acid-sodium acetate buffer (pH 4.0) at temperatures ranging from 30 to 80 • C. To assess the thermostability, Ghlac was incubated at 50 • C and 60 • C in 20 mM phosphate buffer (pH 7.4) for various time intervals, and the residual activity was measured. The Thermofluor assay was performed using a 96-well Applied Biosystems QuantStudio7Flex qPCR instrument (ThermoFisher Scientific, Waltham, MA, USA). The mixture containing 0.3 mg/mL of Ghlac, 5 × Sypro orange, and 100 mM citrate buffer (pH 4.0) was incubated for 10 min on ice before the heating process. The temperature was programmed to increase from 25 to 95 • C at a rate of 1 • C/min in a step-and-hold manner, and the fluorescence in the x1-m2 channel was recorded. The melting temperature (T m ) was calculated by fitting the fluorescence data to the Boltzmann equation. To examine the effect of copper ions on the thermostability of Ghlac, CuSO 4 was added to the mixture, and T m of Ghlac with Cu 2+ was determined.

Effects of Metal Ions and Organic Solvents on the Activity of Mut2
Different concentrations of metal ions (1 mM and 10 mM) and organic solvents (10% and 20%, v/v) were added to the reaction system to study their effects on the activity of Ghlac Mut2. After pre-incubation of Mut2 with metal ions (Na + , Mg 2+ , Ca 2+ , Mn 2+ , Ni 2+ , Zn 2+ , Ba 2+ , and EDTA) and organic solvents (formaldehyde, methanol, ethanol, acetone, isopropanol, and dimethyl sulfoxide) at 4 • C for 30 min, the laccase activity was determined according to the standard laccase assay method.

MG Decolorization Catalyzed by Mut2
The primary decolorization mixture contained 40 U/L of Mut2, 100 mg/L of MG, 1 mM CuSO 4 , and 20 mM acetic acid-sodium acetate buffer (pH 4.0). Decolorization was performed at 60 • C for 2 h in the dark without shaking. The absorbance at 617 nm was measured to detect MG decolorization using Formula (1): where A 0 is the absorbance of untreated MG and A is the absorbance of MG treated by Mut2. The optimal condition of MG decolorization catalyzed by Mut2 was determined. The effect of mediators on MG decolorization was analyzed by adding 0.01 mM of different mediators (ABTS, HBT, VA, MeS, and ASG) to the primary decolorization mixture. The concentration of ABTS (0.01-0.4 mM) was then optimized. The optimal pH was determined in different pH buffers (pH 3.0-5.5) with 0.1 mM ABTS. The optimal temperature was determined at 45 to 85 • C in the pH 4.0 buffer containing 0.1 mM ABTS. The time course of MG decolorization catalyzed by Mut2 in the presence of ABTS was recorded in the pH 4.0 buffer at 70 • C for 3 h.

Toxicity Tests
The bacteria (E. coli and B. subtilis) and plant (Z. mays) seeds were used to evaluate the toxicity of 100 mg/L of MG before and after treatment by Mut2 at 70 • C for 3 h. The pH of the samples was adjusted to 7.0 to eliminate the effect of pH on the growth of bacteria and plant seeds.
The toxicity to bacteria was assessed using the gram-negative bacteria E. coli and the gram-positive bacteria B. subtilis [51]. The samples (4.0 mL) before and after Mut2 treatment were mixed with 5 × LB medium (1.0 mL). The bacteria were inoculated to the mixed solution and incubated at 37 • C. The bacteria growing in 1 × LB medium diluted from 5 × LB medium with distilled water were set as the control in parallel. The absorbance at 600 nm was measured to detect the growth of bacteria.
The phytotoxicity assay using Z. mays seeds was performed according to the previous method [52]. The samples before and after Mut2 treatment were added to the Petri dishes containing the double-layered filter paper. The seeds were placed in the Petri dish and incubated at 25 • C for 4 d in the dark. The seeds growing in distilled water were set as the control in parallel. The lengths of the plumule and radicle were recorded, and the germination rate was calculated according to the following Formula (2): Germination rate (%) = n/N × 100 (2) where n is the number of germinated seeds and N is the number of total tested seeds.

Sequence Analysis
The characteristics of Ghlac (molecular weight and pI) were determined using the ProtParam tool available on the Expasy server (https://web.expasy.org/protparam/, accessed on 15 June 2019). Multiple sequence alignment was performed using Clustal Omega (accessed on 18 May 2020) [53]. The structural model of Ghlac was constructed using SWISS-MODEL (accessed on 18 May 2020) [54] and analyzed using Pymol [55].

Statistical Analysis
All experiments were performed at least three times, and the data were presented as mean ± standard deviation (SD). Microsoft Excel version 2016 (Microsoft Corporation, Redmond, WA, USA) and GraphPad Prism 8.0 (San Diego, CA, USA) were used for all statistical analysis.

Conclusions
Ghlac, a novel member of the DUF152 family, was cloned from G. hydrogeniphilus and characterized as a functional laccase. By the structural and biochemical analyses, the conserved residues H78, C119, and H136 were identified to form the putative copper binding site. In addition, the thermostable Ghlac variant Mut2 was highly tolerant to alkaline conditions and organic solvents. Furthermore, Mut2 could efficiently decolorize MG and thoroughly eliminate the toxicity of MG in the presence of ABTS at high temperatures, showing great potential to remediate MG effluent immediately discharged from the dyeing process. However, further studies on the catalytic mechanism of Mut2 and co-immobilization of laccase and mediator need to be done to facilitate its industrial application.