Enhancing the Physiochemical Properties of Puerarin via L-Proline Co-Crystallization: Synthesis, Characterization, and Dissolution Studies of Two Phases of Pharmaceutical Co-Crystals

Puerarin (PUE) is a Chinese traditional medicine known to enhance glucose uptake into the insulin cells to downregulate the blood glucose levels in the treatment of type II diabetes. Nevertheless, the bioavailability of pristine PUE is limited due to its poor solubility and low intestinal permeability. In this work, we demonstrate that the solubility of PUE can be significantly enhanced via its co-crystallization with L-Proline (PRO). Two crystalline phases, namely, the solvate-free form [PUE][PRO] (I) and the solvated form [PUE]2[PRO]∙EtOH∙(H2O)2 (II) are isolated. These two phases are characterized by single-crystal X-ray diffraction (SCXRD), powder X-ray diffraction (PXRD), Fourier-transformed infrared (FT-IR) spectra, nuclear magnetic resonance (NMR), and thermogravimetric analysis in association with differential scanning calorimetry (TGA-DSC). The solubility and dissolution rate of both I and II in water, gastrointestinal tract at pH 1.2, and phosphate buffer at pH 6.8 indicates a nearly doubled increase as compared to the pristine PUE. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay of pristine PUE, I and II against murine colon cancer cell lines CT-26 and human kidney cell lines HEK-293 indicated that neither compound exhibits obvious cytotoxicity after 24 h. This work showcases that the readily available and biocompatible PRO can be a promising adjuvant to enhance the physicochemical properties of PUE toward orally administered drug formulation with improved pharmacokinetics.


Introduction
One of the primary goals in pharmaceutical crystallization is to increase the solubility of the otherwise less soluble drugs to improve the bioavailability of the active pharmaceutical ingredients (APIs) toward the better pharmacokinetic outcome [1][2][3]. The core of such endeavor underlies the exploitation of different forms of APIs, such as co-crystals, salts, solvates (including hydrates), and polymorphs, followed by their physicochemical properties optimization [4]. Among these forms, co-crystallization is widely used to optimize the physicochemical properties of APIs due to the retention of its intrinsic pharmaceutical properties [5][6][7][8][9][10][11][12]. The pharmacokinetic properties can be regulated through shaping the co-crystals to improve its solubility and dissolution rate [13][14][15][16], mechanical stability [17], hygroscopicity [18], and ultimately bioavailability [10]. In this work, we demonstrate that the solubility of PUE can be readily improved via its co-crystallization with L-proline (PRO, Scheme 1). PRO is well-known to exhibit good potential for co-crystal formation due to the presence of both hydrogen-bonding donor and acceptor sites in the molecule [32][33][34]. Two different phases of PUE-PRO co-crystals, namely the solvate-free form [PUE][PRO] (I) and the solvated form [PUE]2[PRO]•EtOH•(H2O)2 (II), have been prepared under different crystallization conditions and characterized spectroscopically. Compounds I and II represent the first cases of PUE-based co-crystals. As compared to those of the pristine PUE, the solubility and dissolution rates of I and II in water (pH 7.0), gastrointestinal tract at pH 1.2, and phosphate buffer at pH 6.8 indicated a nearly doubled increase. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay of pristine PUE, I and II against murine colon cancer cell lines CT-26 and human kidney cell lines HEK-293 indicated that neither compound exhibits obvious cytotoxicity upon culturing the cells for an extended time of 24 h and 48 h, respectively. This work demonstrates that PUE co-crystal forms of I and II with the presence of readily available and biocompatible PRO can be a promising alternative to pristine PUE with better pharmacokinetic outcomes. In this work, we demonstrate that the solubility of PUE can be readily improved via its co-crystallization with L-proline (PRO, Scheme 1). PRO is well-known to exhibit good potential for co-crystal formation due to the presence of both hydrogen-bonding donor and acceptor sites in the molecule [32][33][34]. Two different phases of PUE-PRO co-crystals, namely the solvate-free form [PUE][PRO] (I) and the solvated form [PUE] 2 [PRO]·EtOH·(H 2 O) 2 (II), have been prepared under different crystallization conditions and characterized spectroscopically. Compounds I and II represent the first cases of PUE-based co-crystals. As compared to those of the pristine PUE, the solubility and dissolution rates of I and II in water (pH 7.0), gastrointestinal tract at pH 1.2, and phosphate buffer at pH 6.8 indicated a nearly doubled increase. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay of pristine PUE, I and II against murine colon cancer cell lines CT-26 and human kidney cell lines HEK-293 indicated that neither compound exhibits obvious cytotoxicity upon culturing the cells for an extended time of 24 h and 48 h, respectively. This work demonstrates that PUE co-crystal forms of I and II with the presence of readily available and biocompatible PRO can be a promising alternative to pristine PUE with better pharmacokinetic outcomes.

Synthesis and Co-Crystal Characterizations
The preferred route for the uptake of drugs, including Chinese traditional medicine (CTM), is the oral administration because of its convenience, low cost, and high persevering acquiescence compared to other routes. However, many of the bioactive ingredients of CTM are poorly soluble, leading to low oral bioavailability and delivery problems. Specifically for PUE, the oral bioavailability is only about 7% [32][33][34][35], and the dominant drug uptake at present is through intravenous injection. Thus, in the current study, the co-crystallization method using L-proline (PRO) as the partner excipient is employed to improve the water solubility of PUE. PRO was employed as a model excipient due to its good biocompatibility, its presence of both donor and acceptor sites for hydrogen-bonding, and its good solubility in water to guarantee the overall solubility of the product [36,37].
Both I and II were prepared as single crystals from equivalent molar of PUE and PRO in EtOH/H 2 O (9:1, v/v) under different precipitation temperatures. In the 1 H nuclear magnetic resonance (NMR) spectra of I and II, peaks in the down-field area (δ 6.79-8.35 ppm) and the up-field area (δ 1.65-2.07 ppm) are diagnostic of the presence of PUE and PRO (Supplementary Figure S1). The triplet at δ 1.05 ppm in II further indicates the inclusion of EtOH solvate within the crystalline sample. The experimental powder X-ray diffraction (PXRD) patterns of I and II agree well with those simulated from single-crystal X-ray diffraction data (Figure 1), indicating highly phase purity of both complexes. In the Fouriertransform infrared (FT-IR) spectra (Supplementary Figure S2), peaks at 1605/1419 cm −1 (I) and 1590/1398 cm −1 (II) were assignable as the asymmetric/symmetric vibrations of COO − in PRO [38][39][40]. Notably, the FT-IR spectra for both I and II feature no peak at around 1700 cm −1 , indicating that the carboxylate remains deprotonated and the proton migrates to the -NH-to give a quaternary amine-carboxylate zwitterionic pair [41], as typically found in proline-based compounds [42,43].

Synthesis and Co-Crystal Characterizations
The preferred route for the uptake of drugs, including Chinese traditional medicine (CTM), is the oral administration because of its convenience, low cost, and high persevering acquiescence compared to other routes. However, many of the bioactive ingredients of CTM are poorly soluble, leading to low oral bioavailability and delivery problems. Specifically for PUE, the oral bioavailability is only about 7% [32][33][34][35], and the dominant drug uptake at present is through intravenous injection. Thus, in the current study, the co-crystallization method using L-proline (PRO) as the partner excipient is employed to improve the water solubility of PUE. PRO was employed as a model excipient due to its good biocompatibility, its presence of both donor and acceptor sites for hydrogen-bonding, and its good solubility in water to guarantee the overall solubility of the product [36,37].
Both I and II were prepared as single crystals from equivalent molar of PUE and PRO in EtOH/H2O (9:1, v/v) under different precipitation temperatures. In the 1 H nuclear magnetic resonance (NMR) spectra of I and II, peaks in the down-field area (δ 6.79-8.35 ppm) and the up-field area (δ 1.65-2.07 ppm) are diagnostic of the presence of PUE and PRO (Supplementary Figure S1). The triplet at δ 1.05 ppm in II further indicates the inclusion of EtOH solvate within the crystalline sample. The experimental powder X-ray diffraction (PXRD) patterns of I and II agree well with those simulated from single-crystal X-ray diffraction data (Figure 1), indicating highly phase purity of both complexes. In the Fouriertransform infrared (FT-IR) spectra (Supplementary Figure S2), peaks at 1605/1419 cm −1 (I) and 1590/1398 cm −1 (II) were assignable as the asymmetric/symmetric vibrations of COO − in PRO [38][39][40]. Notably, the FT-IR spectra for both I and II feature no peak at around 1700 cm −1 , indicating that the carboxylate remains deprotonated and the proton migrates to the -NH-to give a quaternary amine-carboxylate zwitterionic pair [41], as typically found in proline-based compounds [42,43].  The thermogravimetric analysis coupled with differential scanning calorimetry (TGA-DSC) reveals that I exhibit no obvious solvate loss until 224 • C (endothermic), followed by dramatic decomposition (Supplementary Figure S3a). This strongly suggests that I crystallizes as a solvate-free form. Meanwhile, the TGA-DSC curves of II show smooth and continuous solvate loss before 150 • C (Supplementary Figure S3b). This is followed by a plateau until 211 • C, at which point the PUE and PRO pair start to decompose. The total weight loss of 5.8% before 211 • C corresponds to the loss of two aqua and one EtOH molecules (calculated 7.8%), and the slight deviation might due to part of the solvate loss (mainly EtOH) during sample preparation.

Crystal Structure Analysis
Phase I crystallizes in the monoclinic space group P2 1 (Table 1) with its asymmetric unit featuring one PUE and one PRO molecules (Figure 2a). In the PRO moiety of I, the two C-O distances are nearly identical (1.249(3) Å and 1.255(3) Å), indicating that the carboxylate is in a conjugated and uncoordinated fashion, and the proton on the -COOH migrates to the -NH to give a zwitterion, corroborating that observed in the FT-IR spectrum.
Such an averaged C-O distances are similar to those zwitterions based on metal-carboxylate pairs, such as trans-[Zn(iso-hmnH) 2 where n is the number of reflections, and p the total number of parameters refined.
Compound I is rich hydrogen-bonding interactions (Supplementary Table S1), and as a result, the PUE molecules are arranged to give a layered structure within the crystallographic ab plane (Figure 2b), and these layers are further stacked along the c direction in such a way that one-dimensional channels along the b direction is generated to accommodate PRO molecules.
Crystal structure analysis revealed that the solvated form of II belongs to the triclinic P1 space group (Table 1), and its asymmetric unit consists of two PUE, one PRO, one EtOH, and two aqua molecules ( Figure 3a). In II, the PRO moiety also remains in its zwitterionic form similar to that identified in I. The two PUE molecules are in a head-to-tail arrangement as supported by hydrogen-bonding. With the additional presence of one PUE, and crystalline solvates EtOH and H 2 O, the hydrogen-bonding interactions in II are more complex than that of I (Supplementary Table S1). Similar to that found in I, PUE is also found to stack within the crystallographic ab plane (Figure 3b). Nevertheless, the presence of EtOH and aqua solvates marginally increased the separations between these layers to finally yield a sandwich-type structure in which the PRO and its hydrogenbonded solvates reside in-between these layers. It is notable that EtOH is associated with PRO via a single hydrogen bond and can be readily extruded from the network without disturbing the overall structural integrity of II. This may serve to explain the slight deviation of experimental weight loss (5.8%) from that of the calculated (7.8%) observed in the TGA-DSC diagram (Supplementary Figure S3b). Compound I is rich hydrogen-bonding interactions (Supplementary Table S1), as a result, the PUE molecules are arranged to give a layered structure within the cry lographic ab plane (Figure 2b), and these layers are further stacked along the c direc in such a way that one-dimensional channels along the b direction is generated to acc modate PRO molecules.
Crystal structure analysis revealed that the solvated form of II belongs to the tric P1 space group (Table 1), and its asymmetric unit consists of two PUE, one PRO, EtOH, and two aqua molecules (Figure 3a). In II, the PRO moiety also remains in its z terionic form similar to that identified in I. The two PUE molecules are in a head-to arrangement as supported by hydrogen-bonding. With the additional presence of PUE, and crystalline solvates EtOH and H2O, the hydrogen-bonding interactions in II more complex than that of I (Supplementary Table S1). Similar to that found in I, PU also found to stack within the crystallographic ab plane (Figure 3b). Nevertheless, the p ence of EtOH and aqua solvates marginally increased the separations between these la to finally yield a sandwich-type structure in which the PRO and its hydrogen-bon solvates reside in-between these layers. It is notable that EtOH is associated with PRO a single hydrogen bond and can be readily extruded from the network without disturb the overall structural integrity of II. This may serve to explain the slight deviation o perimental weight loss (5.8%) from that of the calculated (7.8%) observed in the TGA-D

Hirshfeld Surface Analysis of TAF, I and II
Hirshfeld surface (HS) analysis serves as a powerful tool to figure out the packing modes and intermolecular interactions in molecular crystals [47]. To evaluate and compare the nature of close interactions between the different lattice components that contributed to the supramolecular assembly of I and II, the three-dimensional (3D) HS and the twodimensional (2D) fingerprint maps were generated using the Crystal Explorer program [48]. As illustrated in Figure 4

Hirshfeld Surface Analysis of TAF, I and II
Hirshfeld surface (HS) analysis serves as a powerful tool to figure out the pack modes and intermolecular interactions in molecular crystals [47]. To evaluate and c pare the nature of close interactions between the different lattice components that cont uted to the supramolecular assembly of I and II, the three-dimensional (3D) HS and two-dimensional (2D) fingerprint maps were generated using the Crystal Explorer p gram [48]. As illustrated in Figure 4

Hirshfeld Surface Analysis of TAF, I and II
Hirshfeld surface (HS) analysis serves as a powerful tool to figure out the pa modes and intermolecular interactions in molecular crystals [47]. To evaluate and pare the nature of close interactions between the different lattice components that co uted to the supramolecular assembly of I and II, the three-dimensional (3D) HS an two-dimensional (2D) fingerprint maps were generated using the Crystal Explore gram [48]. As illustrated in Figure 4   A summary of intermolecular contacts for PUE and PRO are illustrated with decomposed fingerprint plots ( Figure 5) to showcase detailed atom-pair close contacts, including H···H, O···H/H···O, and C···H/H···C contacts. Intermolecular interactions appear as discrete spikes in the 2D fingerprint plots. The quantitative analysis ( Figure 6) shows that the H···H and O···H/H···O interactions are the largest contributors to the total HS for I and II as compared to C···H/H···C and other contacts, represented by the large surfaces (blue) in the fingerprint plots.

Solubility and Dissolution Rates of PUE, I and II
It is well-established that the solid form of APIs has extensive influence on the solubility and dissolution rate of the drug. Consequently, it is vital to choose a suitable API solid form for successful drug expansion. According to the biopharmaceutics classification system (BCS) system, PUE is categorized as a class IV drug, meaning low solubility and low intestinal permeability. The supersaturation solid form of API may increase the bioavailability and permeability.
The comprehensive solubility of PUE, I, and II in the gastrointestinal tract at pH 1.2, phosphate buffer at pH 6.8, and H2O at pH 7.0 are depicted in Figure 7 as well as in Tables S2 and S3 (Supplementary Material). Both I and II exhibit nearly doubled solubility as compared to PUE with the solvated-free form I been comparable to the solvated form of II. In contrast, II also exhibits higher dissolution rate than both I and PUE. The exceptional solubility of I and II, and dissolution rate of II as compared to I and PUE might due to the presence of rich hydrogen-bonding interactions that enables simultaneously more competitive interactions with the solution media to facilitate the dissociation of the subcomponents of the co-crystal. Besides, the sandwich-type structure of II with solvate-accessible layer separations (Figure 3b) as compared to the channel structure of I may also result in the quick entrance of solution media into the crystal lattice to accelerate the dissolution of II. Thus, the increase of dissolution can be observed in the order II > I > PUE, and the

Solubility and Dissolution Rates of PUE, I and II
It is well-established that the solid form of APIs has extensive influence on th bility and dissolution rate of the drug. Consequently, it is vital to choose a suitab solid form for successful drug expansion. According to the biopharmaceutics clas tion system (BCS) system, PUE is categorized as a class IV drug, meaning low sol and low intestinal permeability. The supersaturation solid form of API may increa bioavailability and permeability.
The comprehensive solubility of PUE, I, and II in the gastrointestinal tract at p phosphate buffer at pH 6.8, and H2O at pH 7.0 are depicted in Figure 7 as well as in S2 and S3 (Supplementary Material). Both I and II exhibit nearly doubled solubi compared to PUE with the solvated-free form I been comparable to the solvated f II. In contrast, II also exhibits higher dissolution rate than both I and PUE. The excep solubility of I and II, and dissolution rate of II as compared to I and PUE might due presence of rich hydrogen-bonding interactions that enables simultaneously more petitive interactions with the solution media to facilitate the dissociation of the su ponents of the co-crystal. Besides, the sandwich-type structure of II with solvate-a ble layer separations (Figure 3b) as compared to the channel structure of I may also in the quick entrance of solution media into the crystal lattice to accelerate the disso of II. Thus, the increase of dissolution can be observed in the order II > I > PUE, a  II-A and II-B), showing that the presence of water molecules in II raises the collective contribution of O···H/H···O amongst these interactions.

Solubility and Dissolution Rates of PUE, I and II
It is well-established that the solid form of APIs has extensive influence on the solubility and dissolution rate of the drug. Consequently, it is vital to choose a suitable API solid form for successful drug expansion. According to the biopharmaceutics classification system (BCS) system, PUE is categorized as a class IV drug, meaning low solubility and low intestinal permeability. The supersaturation solid form of API may increase the bioavailability and permeability.
The comprehensive solubility of PUE, I, and II in the gastrointestinal tract at pH 1.2, phosphate buffer at pH 6.8, and H 2 O at pH 7.0 are depicted in Figure 7 as well as in Tables S2 and S3 (Supplementary Material). Both I and II exhibit nearly doubled solubility as compared to PUE with the solvated-free form I been comparable to the solvated form of II. In contrast, II also exhibits higher dissolution rate than both I and PUE. The exceptional solubility of I and II, and dissolution rate of II as compared to I and PUE might due to the presence of rich hydrogen-bonding interactions that enables simultaneously more competitive interactions with the solution media to facilitate the dissociation of the subcomponents of the co-crystal. Besides, the sandwich-type structure of II with solvate-accessible layer separations (Figure 3b) as compared to the channel structure of I may also result in the quick entrance of solution media into the crystal lattice to accelerate the dissolution of II. Thus, the increase of dissolution can be observed in the order II > I > PUE, and the solubility and dissolution of PUE were successfully improved via co-crystallization with PRO.
solubility and dissolution of PUE were successfully improved via co-crystallization with PRO. Figure 7. A summary of the solubility profile of PUE, I and II in the gastrointestinal tract at pH 1.2, phosphate buffer at pH 6.8, and H2O at pH 7.0 (a), as well as the time-dependent dissolution profile of these compounds under respective conditions: gastrointestinal tract at pH 1.2 (b), phosphate buffer at pH 6.8 (c), and H2O at pH 7.0 (d), reflecting the dissolution rate of each compound. Data are expressed as average values from three replicates.

MTT Assays
PUE is widely reported to inhibit the proliferation of various cancer cells such as colon cancer [23][24][25]. It is reported that PUE is capable of inducing the apoptosis of colon cancer HT-29 cells [49]. To further evaluate the potential of PUE, I, and II as anti-cancer agents, we evaluated the biological activities of the pristine PUE, I, and II against the proliferation of CT-26 cancer cell lines (murine origin) and HEK-293 (human origin) cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. As shown in Figure 8, PUE, I, and II exhibit negligible cytotoxicity toward CT-26 and HEK-293 cell lines upon culturing the cells with the drugs for 24 h. These complexes are also comparatively more toxic toward HEK-293 than CT-26 cell lines, and the CT-26 cells were also well survived upon incubating for additional 24 h (Supplementary Figure S4).

MTT Assays
PUE is widely reported to inhibit the proliferation of various cancer cells such as colon cancer [23][24][25]. It is reported that PUE is capable of inducing the apoptosis of colon cancer HT-29 cells [49]. To further evaluate the potential of PUE, I, and II as anti-cancer agents, we evaluated the biological activities of the pristine PUE, I, and II against the proliferation of CT-26 cancer cell lines (murine origin) and HEK-293 (human origin) cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. As shown in Figure 8, PUE, I, and II exhibit negligible cytotoxicity toward CT-26 and HEK-293 cell lines upon culturing the cells with the drugs for 24 h. These complexes are also comparatively more toxic toward HEK-293 than CT-26 cell lines, and the CT-26 cells were also well survived upon incubating for additional 24 h (Supplementary Figure S4).

General
Puerarin (PUE) and L-Proline (PRO) were respectively purchased from J&K Scientific Ltd (Beijing, China) and Aladdin (Shanghai, China). Roswell Park Memorial Institute (RPMI) 1640 cell culture medium (for CT-26) and Dulbecco's modified Eagle medium  [53]. Crystallographic data for the crystal structures have been deposited in the Cambridge Crystallographic Data Centre (CCDC) with supplementary numbers of 2041676 (I) and 2041677 (II). These data can be obtained free of charge from the CCDC via www.ccdc.cam.ac.uk/data_request/cif. A summary of the key crystallographic data are listed in Table 1.

Solubility and Dissolution Measurement
The solubility and dissolution studies of pure PUE, I, and II were measured using a Thermo Scientific Evolution 300 UV spectrometer in deionized water at pH 7.0, gastrointestinal tract at pH 1.2, and phosphate buffer at pH 6.8 solution at 37 • C. The concentration was calculated utilizing a standard curve, which was made by measuring the absorbance of different concentrations at their λ max . A superfluity sum of the sample was added to 10 mL of H 2 O medium, pH 1.2 and pH 6.8 phosphate, respectively.
The oversaturated solution was stirred at 500 RPM by magnetic stirring at 37 • C for 24 h and the suspension was filtered through Whatman's 0.45 mm syringe channel, diluted sufficiently and the absorbance was measured at their λ max . For the dissolution rate, the sample was specifically poured into 250 mL pH 7.0 water, pH 1.2 gastrointestinal tract, and pH 6.8 phosphates medium and then stirred at 150 RPM for 2h at 37 • C. Each solution was measured by the absorbance at λ max . At customary interims, the solution was replenished with 3 mL of the respective blank solution to maintain an overall steady volume.

Cytotoxicity Evaluation by MTT Assay
CT-26 and HEK-293 cell line was purchased from American Type Culture Collection (Manassas, VA, USA). The cell lines were cultured in RPMI 1640 (for CT-26) or DMEM (for HEK-293) containing 10% fetal bovine serum (FBS, Zhejiang Tianhang Biotechnology Co. Ltd., Hangzhou, China) and 1% penicillin/streptomycin (Zhejiang Tianhang Biotechnology Co. Ltd., Hangzhou, China). Cells grew as a monolayer and were detached upon confluence using trypsin (0.5% w/v in PBS, Zhejiang Tianhang Biotechnology Co. Ltd., Hangzhou, China). The cells were harvested from the cell culture medium by incubating in trypsin solution for 3 min. The cells were centrifuged, and the supernatant discarded. A 3 mL portion of serum-supplemented cell culture medium was added to neutralize any residual trypsin. The cells were re-suspended in serum-supplemented RPMI 1640 (for CT-26) or DMEM (for HEK-293) at a concentration of 5 × 10 4 cells per 1 mL. Cells were cultured at 37 • C and 5% CO 2 for MTT studies.
The CT-26 or HEK-293 cells were seeded at a density of 1 × 10 4 cells per well in 100 µL of complete RPMI 1640 (CT-26) or DMEM (for HEK-293), and cultured for 16 h for attachment. The culture medium was then replaced by a serum-free medium containing various concentrations of pristine PUE, I, and II. After incubation with a period of 24 h and 48 h, the MTT solution (100 µL, 0.5 mg/mL in serum-free RPMI 1640 medium for CT-26 and DMEM for HEK-293) was added to replace the cell culture medium. After incubating the cells at 37 • C for 4 h, the MTT solution was removed and DMSO (100 µL) added to dissolve the formazan crystals formed, and the microplates were agitated for 5 min at a medium rate before the spectrophotometric measurement at a wavelength of 570 nm on a microplate reader. The untreated cells served as the 100% cell viability control, while the completely dead cells served as the blank. All experiments were carried out with four replicates. The relative cell viability (%) related to control cells was calculated by the formula below: where V% is the percentage of cell viability, [A] experimental is the absorbance of the wells culturing the treated cells, [A] blank is the absorbance of the blank, and [A] control is the absorbance of the wells culturing untreated cells.

Conclusions
Two new forms of PUE have been successfully obtained and structurally authenticated via its co-crystallization with abundant and naturally-occurring PRO. The solubility and dissolution data suggested that the obtained co-crystal forms I and II exhibit marginal enhancement as compared to the pristine PUE. The solvated form of II further outperforms that of I probably due to its rich hydrogen-bonding interactions and sandwich-type structure that facilitate the quick diffusion of solution media. The use of the bio-compatible and non-toxic natural product as a co-delivery reagent may point to new directions in pharmaceutical co-crystallization to produce drug formulations with synergistic functions.