BZR1 Physically Interacts with SPL9 to Regulate the Vegetative Phase Change and Cell Elongation in Arabidopsis

As sessile organisms, the precise development phase transitions are very important for the success of plant adaptability, survival and reproduction. The transition from juvenile to the adult phase—referred to as the vegetative phase change—is significantly influenced by numbers of endogenous and environmental signals. Here, we showed that brassinosteroid (BR), a major growth-promoting steroid hormone, positively regulates the vegetative phase change in Arabidopsis thaliana. The BR-deficient mutant det2-1 and BR-insensitive mutant bri1-301 displayed the increased ratio of leaf width to length and reduced blade base angle. The plant specific transcription factors SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) are key masters for the vegetative phase transition in plants. The expression levels of SPL9, SPL10 and SPL15 were significantly induced by BR treatment, but reduced in bri1-116 mutant compared to wild-type plants. The gain-of-function pSPL9:rSPL9 transgenic plants displayed the BR hypersensitivity on hypocotyl elongation and partially suppressed the delayed vegetative phase change of det2-1 and bri1-301. Furthermore, we showed that BRASSINAZOLE-RESISTANT 1 (BZR1), the master transcription factor of BR signaling pathway, interacted with SPL9 to cooperatively regulate the expression of downstream genes. Our findings reveal an important role for BRs in promoting vegetative phase transition through regulating the activity of SPL9 at transcriptional and post-transcriptional levels.


Introduction
The transition from the juvenile to adult stage is referred to as the vegetative phase change and is crucial for the reproductive success and survival of higher plants [1]. In Arabidopsis, the change of vegetative stage can be characterized by the appearance of abaxial trichomes, increasing the complexity of leaf shape and the ratio of leaf length to width [2,3]. The microRNA 156 (miR156) and its target transcription factors SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) are the main regulatory factors of the vegetative phase transition in plants [4,5]. A wide range of signals, including sugar, gibberellin acid (GA), abscisic acid (ABA), auxin and endogenous epigenetic factors, regulate the activity of the miR156-SPL module to modulate the vegetative phase change [3,[6][7][8]. These interconnected signal transduction networks integrate multiple developmental, environmental and hormonal signals to precisely optimize the vegetative developmental phase transition in the changing environmental conditions. MicroRNA156 (miR156) is an evolutionarily conserved miRNA, which determines the juvenile-to-adult transition and regulates diverse aspects of plant growth and development [9][10][11][12]. MiR156 is enriched in the juvenile-stage leaves and gradually declines as the shoot develops, while the expression levels of SPLs are reversed, which are abundant etative growth of plants by increasing the activity of SPL9 at both transcriptional and post-transcription levels.

BZR1 Interacts with SPL9 In Vitro and In Vivo
To further investigate the function of BZR1 in plant growth and development, we screened the proteins interacting with BZR1 by Yeast two-hybrid (Y2H) system. SPL9 was identified from the putative BZR1-interacting candidates. Additional Y2H assays showed that SPL9 interacted with BZR1 and BES1 in yeast ( Figure 1A). BZR1 contains the amino-terminal DNA binding domain, PEST domain, which is responsible for the interaction with PP2A, and the carboxy-terminal domain, which itself is critical for the association with BIN2. The Y2H assays showed that SPL9 had the high binding ability with BZR1-C, weak binding ability with PEST domain, and did not interact with BZR1-N ( Figure 1B,C). To confirm the interaction between BZR1 and SPL9, protein-protein pull down assay was performed using purified proteins fused glutathione S transferase (GST) or maltose binding protein (MBP) expressed from Escherichia coli. The results showed that GST-SPL9 protein pulled down MBP-fused BZR1, but not MBP alone ( Figure 1D). To determine whether BZR1 interacts with SPL9 in plants, transient bimolecular fluorescence complementation (BiFC) assays were carried out in the tobacco leaves. The results showed that the strong fluorescent signals were detected in the nucleus of the epidermal cells of tobacco leaves when BZR1-cYFP was co-transformed with SPL9-nYFP, but not with control x-nYFP ( Figure 1E). Furthermore, we performed Co-IP assay using YFP-trap beads and Arabidopsis protoplast, in which co-expressing 35S:GFP and 35S:bzr1-1D-MYC or coexpressing 35S:SPL9-GFP and 35S:bzr1-1D-MYC. The results showed that BZR1 interacted with SPL9 in plants ( Figure 1F). All these results proved that BZR1 interacts with SPL9 in vitro and in vivo.

Enhancing the Activity of SPL9 Led to the BR Hypersensitivity
In Arabidopsis, several members of the SPL family are regulated post-transcriptionally by miR156. To determine whether the miR156 and SPL9 are involved in the BR signaling pathway, we studied the BR response of wild type, miR156B-Ox, in which miR156B was expressly driven by 35S promoter, the miR156 target mimics line (MIM156) that reduces miR156 activity, and pSPL9:rSPL9 plants where the resistant SPL9 (rSPL9) nontargeted by miR156 was expressed under its native promoter. The results showed that BR promoted the hypocotyl elongation of wild-type plants in a dose-dependent manner under the consistent light condition. The miR156B-Ox and spl9/spl15 displayed the BR response similar to that of wild-type plants, but the MIM156 and pSPL9:rSPL9 displayed hypersensitivity to BR compared to wild-type plants (Figure 2A-C and Figure S1A-C). To determine the BR response of miR156/SPL9 module, we grew wild type, miR156B-Ox and pSPL9:rSPL9 on the 1 2 MS medium containing different concentrations of BR biosynthesis inhibitor propiconazole (PPZ). We found miR156B-Ox displayed much smaller rosette leaves than wild-type plants, and pSPL9:rSPL9 showed significantly increased rosette leaf sizes ( Figure S2). Furthermore, the bzr1-1D gain-of-function mutant displayed the PPZ-resistant phenotypes, but such PPZ resistance of bzr1-1D were significantly reduced by miR156 overexpression ( Figure S3). These results indicated that the plants with high activity of SPL9 are hypersensitive to BR. (F) BZR1 interacts with SPL9 in vivo. Immunoprecipitation (IP) was performed using Arabidopsis protoplast co-expressing 35S:GFP and 35S:bzr1-1D-MYC or co-expressing 35S:SPL9-GFP and 35S:bzr1-1D-MYC. The coimmunoprecipitation experiments were performed using GFP-Trap agarose beads, and the immunoblots were probed with anti-Myc or anti-YFP antibodies.

Enhancing the Activity of SPL9 Led to the BR Hypersensitivity
In Arabidopsis, several members of the SPL family are regulated post-transcriptionally by miR156. To determine whether the miR156 and SPL9 are involved in the BR signaling pathway, we studied the BR response of wild type, miR156B-Ox, in which miR156B was expressly driven by 35S promoter, the miR156 target mimics line (MIM156) that reduces miR156 activity, and pSPL9:rSPL9 plants where the resistant SPL9 (rSPL9) nontargeted by miR156 was expressed under its native promoter. The results showed that BR promoted the hypocotyl elongation of wild-type plants in a dose-dependent manner under the consistent light condition. The miR156B-Ox and spl9/spl15 displayed the BR response similar to that of wild-type plants, but the MIM156 and pSPL9:rSPL9 displayed hypersensitivity to BR compared to wild-type plants ( Figure  2A-C and Figure S1A-C). To determine the BR response of miR156/SPL9 module, we grew wild type, miR156B-Ox and pSPL9:rSPL9 on the ½ MS medium containing different
concentrations of BR biosynthesis inhibitor propiconazole (PPZ). We found miR156B-Ox displayed much smaller rosette leaves than wild-type plants, and pSPL9:rSPL9 showed significantly increased rosette leaf sizes ( Figure S2). Furthermore, the bzr1-1D gain-of-function mutant displayed the PPZ-resistant phenotypes, but such PPZ resistance of bzr1-1D were significantly reduced by miR156 overexpression ( Figure S3). These results indicated that the plants with high activity of SPL9 are hypersensitive to BR.

BR Ppromotes the Change of Vegetative Phase in Arabidopsis
The miR156-SPL9 module has been reported to play a critical role in the progression from the juvenile phase to the adult phase in plants. Considering that BZR1 interacts with SPL9 to regulate cell elongation, we want to determine whether BR and BZR1 participate in the vegetative phase change in plants. The BR-deficient mutants det2-1, and BR-insensitive mutants bri1-301, and the constitutively active forms bin2-1 all displayed the increased ratio of leaf width to length, and the decreased blade base angle ( Figure 4A-C). Meanwhile the BR signal enhanced materials such as BRI1 overexpression (BRI1-Ox), DWF4 overexpression (DWF4-Ox), and bzr1-1D overexpression (bzr1-1D-Ox), showed the decreased ratio of leaf width to length and the increased blade base angle ( Figure 4A-C). These results indicated that BR promotes the vegetative phase change. Error bars represent standard deviation. Different letters above the bars indicated statistically significant differences between the samples (ANOVA analysis followed by Uncorrected Fisher's LSD (Least-significant difference) multiple comparisons test, p < 0.05). (C,D) Seedlings of wild type and indicated plants were grown in the dark for 7 days. Hypocotyl lengths were measured from at least 30 plants. Error bars represent standard deviation. Different letters above the bars indicated statistically significant differences between the samples (ANOVA analysis followed by Uncorrected Fisher's LSD multiple comparisons test, p < 0.05).

BR Ppromotes the Change of Vegetative Phase in Arabidopsis
The miR156-SPL9 module has been reported to play a critical role in the progression from the juvenile phase to the adult phase in plants. Considering that BZR1 interacts with SPL9 to regulate cell elongation, we want to determine whether BR and BZR1 participate in the vegetative phase change in plants. The BR-deficient mutants det2-1, and BR-insensitive mutants bri1-301, and the constitutively active forms bin2-1 all displayed the increased ratio of leaf width to length, and the decreased blade base angle ( Figure  4A-C). Meanwhile the BR signal enhanced materials such as BRI1 overexpression (BRI1-Ox), DWF4 overexpression (DWF4-Ox), and bzr1-1D overexpression (bzr1-1D-Ox), showed the decreased ratio of leaf width to length and the increased blade base angle ( Figure 4A-C). These results indicated that BR promotes the vegetative phase change. To determine the role of SPL9 in BR-mediated vegetative phase change, we described the growth phenotypes of wild type, det2-1, bri1-301, pSPL9:rSPL9, pSPL9:rSPL9/det2-1 and pSPL9:rSPL9/bri1-301. The results showed of det2-1 and bri1-301 have a bigger ratio of leaf width to length and smaller blade base angle, while pSPL9:rSPL9 have a smaller ratio of leaf width to length and bigger blade base angle (Figures 5A-F and S4A-F). What's more, the rounder leaves of det2-1 and bri1-301 were suppressed by pSPL9:rSPL9 ( Figures 5C,  and S4C). In addition, the pSPL9:rSPL9 can partially affect the rosette leaf numbers and leaf growth rate of det2-1 and bri1-301 ( Figures 5B,D and S4B,D). Furthermore, we found that Col-0 appeared the abaxial trichome in the 7th rosette leaf, while in bri1-301 there appeared the abaxial trichome in the 9th leaf, significantly delaying the appearance of abaxial trichomes in the pSPL9:rSPL9 plant ( Figure S5). These results indicated that SPL9 promotes vegetative phase change downstream of BR.  pSPL9:rSPL9 have a smaller ratio of leaf width to length and bigger blade base angle ( Figure 5A-F, Figure S4A-F). What's more, the rounder leaves of det2-1 and bri1-301 were suppressed by pSPL9:rSPL9 ( Figure 5C, Figure S4C). In addition, the pSPL9:rSPL9 can partially affect the rosette leaf numbers and leaf growth rate of det2-1 and bri1-301 ( Figure  5B,D, Figure S4B,D). Furthermore, we found that Col-0 appeared the abaxial trichome in the 7th rosette leaf, while in bri1-301 there appeared the abaxial trichome in the 9th leaf, significantly delaying the appearance of abaxial trichomes in the pSPL9:rSPL9 plant ( Figure S5). These results indicated that SPL9 promotes vegetative phase change downstream of BR.

BR Induces the Expression of Several SPL Genes
Given the important roles of BR on the progression from the juvenile phase to the adult phase, we speculated that BR regulate the expression of miR156 and SPL genes. To test this hypothesis, we analyzed the expression levels of SPL genes in wild type and BR-insensitive mutant bri1-116. Quantitative RT-PCR analysis showed that the expression levels of SPL9, SPL10 and SPL15 were significantly reduced in bri1-116 mutant ( Figure 6A). To further analyze the effects of BR on the expression of SPL genes, we analyzed the transcript levels of SPL genes in wild type with or without BR treatment. The results showed that BR treatment induced the expression of SPL9, but had no significant effects on the expression of SPL10 and SPL15 in wild type plants ( Figure 6B). In order to eliminate the effects of miR156 on the expression of SPL genes, we analyzed the regulation of BR on the expression of SPLs in MIM156 plants. The expression levels of SPL9, SPL10 and SPL15 were significantly increased in MIM156 compared to that in wild-type plants ( Figure 6B). These results revealed that BR induces the expression of SPL9, SPL10 and SPL15. ted plants. The leaf width and leaf length were measure from at least 30 plants. Error bars represent standard deviation. ) The base angle of full expanded rosette leaves of wild type and indicated plants. The leaf base angle were measure om at least 30 plants. Error bars represent standard deviation.

BR Induces the Expression of Several SPL Genes
Given the important roles of BR on the progression from the juvenile phase to the adult phase, we speculated that BR regulate the expression of miR156 and SPL genes. To test this hypothesis, we analyzed the expression levels of SPL genes in wild type and BR-insensitive mutant bri1-116. Quantitative RT-PCR analysis showed that the expression levels of SPL9, SPL10 and SPL15 were significantly reduced in bri1-116 mutant ( Figure 6A). To further analyze the effects of BR on the expression of SPL genes, we analyzed the transcript levels of SPL genes in wild type with or without BR treatment. The results showed that BR treatment induced the expression of SPL9, but had no significant effects on the expression of SPL10 and SPL15 in wild type plants ( Figure 6B). In order to eliminate the effects of miR156 on the expression of SPL genes, we analyzed the regulation of BR on the expression of SPLs in MIM156 plants. The expression levels of SPL9, SPL10 and SPL15 were significantly increased in MIM156 compared to that in wild-type plants ( Figure 6B). These results revealed that BR induces the expression of SPL9, SPL10 and SPL15. Asterisk between bars indicated statistically significant differences between the samples (Student t test, * p < 0.05). (B) BR increased the transcript of SPL9, SPL10 and SPL15. Seedlings of wild type and MIM156 were grown on ½ MS medium for 7 days, and then treated with 100 nM BL for 3 h. PP2A gene was analyzed as an internal control. Error bars represent standard deviation of three independent experiments. Different letters above the bars indicated statistically significant differences between the samples (ANOVA analysis followed by Uncorrected Fisher's LSD multiple comparisons test, p < 0.05).

BZR1 and SPL9 Coordinately Regulate the Expression of Downstream Genes
The HLH transcription factors PRE promote cell elongation participating in various hormones and environmental signals. Our previous study showed that BZR1 directly binds to the promoters of PREs to induce their expression. To examine whether SPL9 promotes cell elongation by regulating the expression of PREs, we performed the quantitative RT-PCR analysis in wild type, det2-1, spl9/spl15, pSPL9:rSPL9 and pSPL9:rSPL9/det2-1. The results showed the expression levels of PRE1, PRE5 and PRE6  1 2 MS medium for 7 days, and then treated with 100 nM BL for 3 h. PP2A gene was analyzed as an internal control. Error bars represent standard deviation of three independent experiments. Different letters above the bars indicated statistically significant differences between the samples (ANOVA analysis followed by Uncorrected Fisher's LSD multiple comparisons test, p < 0.05).

BZR1 and SPL9 Coordinately Regulate the Expression of Downstream Genes
The HLH transcription factors PRE promote cell elongation participating in various hormones and environmental signals. Our previous study showed that BZR1 directly binds to the promoters of PREs to induce their expression. To examine whether SPL9 promotes cell elongation by regulating the expression of PREs, we performed the quantitative RT-PCR analysis in wild type, det2-1, spl9/spl15, pSPL9:rSPL9 and pSPL9:rSPL9/det2-1. The results showed the expression levels of PRE1, PRE5 and PRE6 were similar in wild type and spl9/spl15, but much higher in pSPL9:rSPL9 lines than that in wild-type plants, suggesting SPL9 induces the expression of PRE1, PRE5 and PRE6 (Figures 7A and S1D). Consistent with previous results, the transcript levels of PRE1, PRE5 and PRE6 were decreased in det2-1 mutants, but these decreased expression levels of these genes were partially suppressed by pSPL9:rSPL9 ( Figure 7A). To test whether BZR1 and SPL9 cooperatively regulate the expression of PREs, we performed transient gene expression analysis by generating promoter-luciferase (LUC) reporter construct with PRE5 promoter in mesophyll protoplasts of Arabidopsis leaves. We observed that the luciferase activity derived from the pPRE5:LUC increased when BZR1 and SPL9 were transfected alone, and significantly induced when BZR1 and SPL9 were co-expressed ( Figure 7B). These results indicated that BZR1 and SPL9 cooperatively induce the expression of PRE5.
whether BZR1 and SPL9 cooperatively regulate the expression of PREs, we performed transient gene expression analysis by generating promoter-luciferase (LUC) reporter construct with PRE5 promoter in mesophyll protoplasts of Arabidopsis leaves. We observed that the luciferase activity derived from the pPRE5:LUC increased when BZR1 and SPL9 were transfected alone, and significantly induced when BZR1 and SPL9 were co-expressed ( Figure 7B). These results indicated that BZR1 and SPL9 cooperatively induce the expression of PRE5. Seedlings of wild type, det2-1, pSPL9:rSPL9 and pSPL9:rSPL9/det2-1 were grown on ½ MS medium under constant light for 7 days. PP2A gene was analyzed as an internal control. Error bars represent standard deviation of three independent experiments. Different letters above the bars indicated statistically significant differences between the samples (ANOVA analysis followed by Uncorrected Fisher's LSD multiple comparisons test, p < 0.05). (B) Transient assays show BZR1 and SPL9 cooperative activation of the pPRE5:LUC reporter gene. The construct containing pPRE5:LUC (luciferase) and 35S:REN (renilla luciferase) and constructs overexpressing the indicated effecters were transfected to Arabidopsis protoplasts simultaneously. The LUC activity was normalized to REN. Error bars indicate SD of three biological repeats. Different letters above the bars indicated statistically significant differences between the samples (ANOVA analysis followed by Uncorrected Fisher's LSD multiple comparisons test, p < 0.05).

Discussion
Plant vegetative phase change is regulated by various of environmental and endogenous signals largely through influencing the activity of miR156-SPL module. Here, we showed that BR play critical roles for the transition from the juvenile to adult stage. BR-deficient mutant det2-1 and BR-insensitive mutant bri1-301 both displayed the round leaves and the increased ratio of leaf width to length, while overexpression of BRI1 or DWF4 exhibited the decreased ratio of leaf width to length. BR treatment significantly increased the expression of SPL9. Activated SPL9 by overexpression of MIM156 or miR156-resistant form rSPL9 resulted in the hypersensitivity to BR and partially suppressed the dwarf phenotypes of det2-1 and bri1-301. Furthermore, we showed that BZR1 interacts with SPL9 to coordinately regulate the expression of downstream genes. Our work reveals that age and BR pathways are integrated to regulate plant growth and development through the direct physical interaction between SPL9 and BZR1.
BRs function as one type of growth-promoting hormones and are biosynthesized in the young tissues to promote plant growth and development [32]. BR biosynthesis defect Seedlings of wild type, det2-1, pSPL9:rSPL9 and pSPL9:rSPL9/det2-1 were grown on 1 2 MS medium under constant light for 7 days. PP2A gene was analyzed as an internal control. Error bars represent standard deviation of three independent experiments. Different letters above the bars indicated statistically significant differences between the samples (ANOVA analysis followed by Uncorrected Fisher's LSD multiple comparisons test, p < 0.05). (B) Transient assays show BZR1 and SPL9 cooperative activation of the pPRE5:LUC reporter gene. The construct containing pPRE5:LUC (luciferase) and 35S:REN (renilla luciferase) and constructs overexpressing the indicated effecters were transfected to Arabidopsis protoplasts simultaneously. The LUC activity was normalized to REN. Error bars indicate SD of three biological repeats. Different letters above the bars indicated statistically significant differences between the samples (ANOVA analysis followed by Uncorrected Fisher's LSD multiple comparisons test, p < 0.05).

Discussion
Plant vegetative phase change is regulated by various of environmental and endogenous signals largely through influencing the activity of miR156-SPL module. Here, we showed that BR play critical roles for the transition from the juvenile to adult stage. BRdeficient mutant det2-1 and BR-insensitive mutant bri1-301 both displayed the round leaves and the increased ratio of leaf width to length, while overexpression of BRI1 or DWF4 exhibited the decreased ratio of leaf width to length. BR treatment significantly increased the expression of SPL9. Activated SPL9 by overexpression of MIM156 or miR156-resistant form rSPL9 resulted in the hypersensitivity to BR and partially suppressed the dwarf phenotypes of det2-1 and bri1-301. Furthermore, we showed that BZR1 interacts with SPL9 to coordinately regulate the expression of downstream genes. Our work reveals that age and BR pathways are integrated to regulate plant growth and development through the direct physical interaction between SPL9 and BZR1.
BRs function as one type of growth-promoting hormones and are biosynthesized in the young tissues to promote plant growth and development [32]. BR biosynthesis defect resulted in delayed plant growth, rounded rosette leaves and the increased the blade base angle, suggesting BR is involved in the vegetative phase change of plants [34,36,50]. However, the molecular mechanism by which BR regulates the vegetative phase change remains unclear. In this study, we showed that BZR1 interacted with SPL9 to integrate the age and BR signaling pathways to regulate the vegetative phase change. Mutation of BR receptor BRI1 resulted in the significant reduced expression levels of SPL9, SPL10 and SPL15, which prolonged the vegetative phase. The round rosette leaves of BR-deficient mutant det2-1 was suppressed by the pSPL9:rSPL9, suggesting SPL9 regulate the vegetative growth of plants downstream of BR signaling. In addition, SPL9 directly interacted with BZR1 to promote the downstream gene expression. These results indicated that BR promotes the vegetative phase change through dual regulation of activity of SPL9 at the transcriptional and post-transcriptional levels.
MiR156 and its target gene SPL transcription factors are reported to regulate a wide range of plant growth and development by modulating the biosynthesis and signal transduction of plant hormones [9,10]. DELLA proteins, the key repressors of Gibberellin acid (GA) signaling pathway, interact with SPL9 to interfere SPL9 transcriptional activity and consequently delay floral transition and inhibit axillary meristem initiation [30,51]. In common wheat (Triticum aestivum), TaSPL8 knock-out mutants displayed the erect leaves and increased spike number in high planting density. TaSPL8 directly binds to the promoters of BR biosynthesis gene CYP90D2 and AUXIN RESPONSE FACTOR to induce their expression, suggesting TaSPL8 might increase lamina joint through auxin signaling and BR biosynthesis [52]. Here, in this study, we showed that the dwarf phenotypes of det2-1 and bri1-301 were partially suppressed by the pSPL9:rSPL9 transgenic plants. The activation by expression rSPL9 or MIM156 resulted in the increased BR sensitivity and overexpression of miR156B partially suppressed the PPZ resistance of bzr1-1D. The spl9/spl15 mutants displayed the BR response similar to that of wild type, which may be due to the functional redundancy of SPL gene family. BR induces the expression of several SPL genes. BZR1, the master regulator of BR signaling pathway, interacts with SPL9 to coordinately regulate the downstream gene expression. These results indicated that SPL9 is a positive regulator of BR signaling pathway, and work together with BZR1 to promote cell elongation and vegetative phase change in Arabidopsis.

Bimolecular Fluorescence Complementation Assays
Full-length coding sequences of SPL9 and BZR1 were fused in-frame with the Nterminal of YFP and C-terminal of YFP, respectively. Agrobacterial suspensions containing SPL9-nYFP or BZR1-cYFP constructs were injected into tobacco (Nicotiana tabacum) leaves simultaneously. The transfected plants were cultivated in the greenhouse for at least 36 h at 22 • C, and then were used to analyze fluorescent signals using an LSM700 laser scanning confocal microscope (Zeiss, Oberkochen, Germany).

RNA Extraction, Reverse Transcription and Real-Time PCR
Total RNA was extracted from wild type and various mutants grown on 1 2 MS medium with 1% sucrose under constant light for 7 days using Trizol RNA extraction kit (TransGen Biotech, Beijing, China). First-strand cDNA were synthesized using RevertAid reverse transcriptase (Thermo Fisher, Waltham, MA, USA). Quantitative PCR analyses were performed on a CFX connect real-time PCR detection system (Bio-Rad, Hercules, CA, USA) using a SYBR green reagent (Roche, Basel, Switzerland) with gene-specific primers.

Transient Gene Expression Assays
Protoplast isolation and PEG transformation was carried out as described previously [56,57]. Protoplasts were harvested by centrifugation and lysed in 100 µL of passive lysis buffer (Promega, Madison, WI, USA). Firefly and Renilla (as internal standard) luciferase activities were measured using a dual-luciferase reporter kit (Promega, Madison, WI, USA).

Conclusions
In summary, we found that BR promotes the vegetative phase change by regulating SPL9 activity at both transcriptional and post-transcriptional levels. BR-deficient orinsensitive mutants displayed the round leaf and reduced leaf blade base angle, whereas BRI1-Ox and DWF4-Ox showed the slender leaf and increased leaf base angle. BR induces the expression of SPLs. SPL9 interacted with BZR1 to coordinately regulate the downstream gene expression. These results demonstrated that SPL9 regulates cell elongation and vegetative phase change downstream of developmental and hormonal signals.