Identification of FadT as a Novel Quorum Quenching Enzyme for the Degradation of Diffusible Signal Factor in Cupriavidus pinatubonensis Strain HN-2

Quorum sensing (QS) is a microbial cell–cell communication mechanism and plays an important role in bacterial infections. QS-mediated bacterial infections can be blocked through quorum quenching (QQ), which hampers signal accumulation, recognition, and communication. The pathogenicity of numerous bacteria, including Xanthomonas campestris pv. campestris (Xcc), is regulated by diffusible signal factor (DSF), a well-known fatty acid signaling molecule of QS. Cupriavidus pinatubonensis HN-2 could substantially attenuate the infection of XCC through QQ by degrading DSF. The QQ mechanism in strain HN-2, on the other hand, is yet to be known. To understand the molecular mechanism of QQ in strain HN-2, we used whole-genome sequencing and comparative genomics studies. We discovered that the fadT gene encodes acyl-CoA dehydrogenase as a novel QQ enzyme. The results of site-directed mutagenesis demonstrated the requirement of fadT gene for DSF degradation in strain HN-2. Purified FadT exhibited high enzymatic activity and outstanding stability over a broad pH and temperature range with maximal activity at pH 7.0 and 35 °C. No cofactors were required for FadT enzyme activity. The enzyme showed a strong ability to degrade DSF. Furthermore, the expression of fadT in Xcc results in a significant reduction in the pathogenicity in host plants, such as Chinese cabbage, radish, and pakchoi. Taken together, our results identified a novel DSF-degrading enzyme, FadT, in C. pinatubonensis HN-2, which suggests its potential use in the biological control of DSF-mediated pathogens.


Introduction
Black rot, caused by Xanthomonas campestris pv. campestris (Xcc), is a worldwide threat to plants. This disease mainly affects the aboveground parts of plants at any growth stage, resulting in a heavy loss of yield. All cruciferous vegetables, including pakchoi cabbage, broccoli, Chinese cabbage, radish, cauliflower, kale, asparagus, mustard, and turnip, are prone to suffering from black rot [1].
Quorum sensing (QS) is used by bacteria to interact with one another and coordinate their group activities. Thus, QS controls a variety of pathogen-mediated diseases in humans, animals, and plants [2]. QS systems are widely distributed in Xcc [3]. After infecting host plants, Xcc produces a series of extracellular enzymes that are important in bacterial pathogenesis. This process is linked to the signaling molecule diffusible signal factor (DSF), which was previously identified as cis-11-methyl-2-dodecenoic acid. Accumulated DSF Int. J. Mol. Sci. 2021, 22, 9862 2 of 18 results in the expression of tissue-macerating pathogenicity genes in Xcc [4,5]. DSF is a member of the QS signaling family, which is involved in the regulation of toxic substances produced by Gram-negative bacteria. Apart from being present in all Xanthomonas species, DSF is also found in Pseudomonas aeruginosa, Burkholderia sp., and marine bacteria. [6]. DSF family members' signals are linked to bacterial toxicity, biofilm development, and pathogen antibiotic tolerance [7]. However, the main means of prevention and control of black rot are chemical pesticides and antibiotics. The excessive application of antibiotics and antimicrobials leads to drug resistance, which threatens nontarget organisms, including humans [8]. Therefore, for the consideration of human health and social security, it is becoming particularly important to find a late-model, effective, and safe prevention and control measure.
The discovery of quorum quenching (QQ) provides new avenues for the prevention and biocontrol of plant diseases. QQ disrupts the density-dependent attack of bacterial population by blocking the cell-cell communication. This novel strategy impairs the virulence of pathogens while not killing them, thus reducing the selective pressure on pathogens and delaying the evolution of QS-mediated drug resistance [9]. QQ has been engineered in plants to control pathogens and establish an active defense barrier [10]. The number of known organisms and enzymes that interfere with QS is growing, paving the way for new antimicrobial strategies to be developed [11].
In recent years, N-acyl homoserine lactone (AHL)-degrading bacteria and their degrading enzymes have undergone extensive studies, and their related catalytic mechanisms have been well characterized [12]. However, there is a scarcity of information about DSF-degrading bacteria and their corresponding enzymes. RpfB has been identified as an inactivation enzyme responsible for DSF degradation in Xcc and Xanthomonas oryzae pv. oryzae (Xoo) [13]. RpfB activates a variety of fatty acids into CoA esters in vitro, and furthermore, RpfB can take the place of the FadD of paradigm bacterial acyl-CoA ligase in the Escherichia coli β-oxidation pathway [14]. Thus far, the genome sequence and molecular mechanism of DSF-degrading strains are not clear, which limits the applications of these QQ strains.
In our previous study, Cupriavidus pinatubonensis strain HN-2, isolated from an agricultural field, exhibited superior DSF degradation activity and completely degraded 2 mM of DSF within 24 h. Moreover, strain HN-2 could reduce the black rot disease caused by Xcc. Application of the HN-2 strain as a biocontrol agent could substantially reduce the disease severity [15]. However, the QQ enzymes and related genes responsible for DSF degradation in strain HN-2 have not been explored. In this research, we identified a novel DSF-degrading enzyme FadT in C. pinatubonensis strain HN-2 through whole-genome sequencing (WGS) and comparative genomics. Our study further evaluated the biocontrol effects of recombinant strain E. coli BL21/pGEX fadT carrying FadT in Chinese cabbage (Brassica pekinensis (Lour.) Rupr.), radish (Raphanus sativus L.), and pakchoi (Brassica campestris L. ssp. chinensis Makino) host plants against DSF-mediated pathogens. Our findings revealed the molecular basis of highly efficient DSF-degrading C. pinatubonensis HN-2 and indicated useful genes and agents that show potential for biocontrol of black rot and other infectious diseases caused by DSF-dependent bacterial pathogens.

Whole-Genome Sequencing and Assembly
In this study, genomic data were used to gain a better understanding of the mechanisms underlying bacteria's DSF-degrading activity. As a result, a whole-genome analysis was performed to identify the entire set of genes involved in DSF degradation. The C. pinatubonensis strain HN-2 was isolated from agricultural soil and deposited in Guangdong Microbial Culture Collection Center (GDMCC), China (collection number: GDMCC NO 60432) [15]. The procedure of extracting the HN-2 genome was as follows: HN-2 was first removed from the −80 • C freezer and activated on LB solid medium for two days at 20 • C. Then, the sodium dodecyl sulfate (SDS) method was used to collect the genomic DNA. Additionally, agarose gel electrophoresis was used to detect the extracted DNA, and Qubit was used to quantify it. The genome of HN-2 was sequenced using single-molecule real-time (SMRT) technology, and the sequencing was done by LC-Bio Technology Co., Ltd., Hangzhou, China. The low-quality reads were filtered using SMRT Link v5.0.1. (https://www.pacb.com/support/software-downloads/, accessed on 30 July 2020), and the reads were filtered to create a single configuration with no gaps [17,18]. The original underlying data were saved in an H5 file that included sequencing sequences, base quality values, and other details. The clean reads available for analysis were obtained by managing the quality of the original subordinate data and deleting low-quality sequences. The following information was obtained: clean reads, the total amount of data, quality value assignment, and long reads. SMRT Link V5.0.1 software was utilized to put together the reads, and Illumina short-read sequencing data were used to further polish the reads. (1) In the PacBio sequel platform, single-molecule real-time (SMRT) sequencing libraries were created with a 10 kb insert size using the SMRTbell Template kit (LC-Bio Technology Co., Ltd., Hangzhou, China), version 1.0. The whole procedure was as follows: Firstly, DNA was fragmented and concentrated. Secondly, damaged DNA ends were repaired, and the blunt ligation reaction was prepared. Then, the SMRTbell Templates were purified with 0.45XAMPure PB Beads. Moreover, the BluePippin System was used to select the size, and the DNA damage was repaired. Finally, a Qubit 2.0 Fluorometer (Thermo Scientific, Waltham, MA, USA) was used to assess the library quality, and Agilent 2100 (Agilent Technologies, Santa Clara, CA, USA) was used to detect the insert fragment size. (2) In the Illumina NovaSeq (Illumina, San Diego, CA, USA) platform, a total of 1 µg DNA from each sample was utilized as the input material for preparing the DNA sample. Sequencing libraries were created using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) according to the manufacturer's instructions, and index codes were assigned to each sample's sequences. In brief, sonication was used to fragment the DNA sample to 350 bp; the fragments were then A-tailed, end-polished, and ligated with a full-length adaptor for Illumina sequencing and further PCR amplification. Finally, PCR products were purified (AMPure XP system, Beckman Coulter, Beverly, CA, USA), and the size distribution of libraries was evaluated using an Agilent 2100 Bioanalyzer and quantified using real-time PCR. The wholeness of the genome assembly was evaluated using BUSCO v4.1.2 (https://busco.ezlab.org, accessed on 30 July 2020) and the Burkholderiales_odb10 benchmarking dataset.
The gene functions of HN-2 were predicted using the following databases: the Kyoto Encyclopedia of Genes and Genomes (KEGG) [29,30], Gene Ontology (GO) [31], Clusters of Orthologous Groups (COG) [32], Protein Families (Pfam), Swiss-Prot [33], the Transporter Classification Database (TCDB) [34], and the Non-Redundant Protein Database (NR) [35]. The above databases were subjected to a whole-genome BLAST search (E-value less than 1 × 10 −5 minimal alignment length >40%), and the genes were annotated by comparing them to the genes in the above-mentioned databases. The Carbohydrate-Active Enzymes (CAZy) database was used to predict the carbohydrate-active enzymes [36].
Secretory proteins and type I-VII proteins released by pathogenic bacteria were predicted using SignalP [37]. Additionally, antiSMASH (version 2.0.2) was used to analyze the secondary metabolism gene clusters (http://antismash.secondarymetabolites.org, accessed on 30 July 2020) [38]. Furthermore, the Pathogen Host Interactions (PHI) database, the Antibiotic Resistance Genes Database (ARDB) [39], and the Virulence Factors of Pathogenic Bacteria (VFDB) database [40] were used for the analysis of drug resistance and pathogenicity [41]. Furthermore, the circular genome data visualization was graphed based on Circos software [42].

Construction of an In-Frame Deletion Mutant and Complementation
To produce deletion mutants, C. pinatubonensis HN-2 wild-type was utilized as a parental strain. A 500 bp upstream and downstream fragments of the fadT gene were amplified by PCR and cloned into a HindIII-BamHI-linearized pK18mobsacB vector. Triparental mating with the helper strain HB101 (RK2013) on an LB plate at 30 • C for 10 h mobilized the recombinant plasmid into strain HN-2. Cells were selected with LB medium supplemented with gentamicin and chloramphenicol. Colonies were further selected on LB plates containing 10% sucrose. ∆fadT mutant was verified by PCR and DNA sequencing. Further, to investigate the inhibitory effect on DSF-mediated Xcc pathogenicity of the fadT in-frame ∆fadT deletion mutants, biocontrol efficacy against black rot in Chinese radish (Raphanus sativus L.) was performed. The bacterial suspensions were inoculated on radish root slices containing suspensions of black rot pathogen Xcc (6 × 10 8 CFU·mL −1 ), suspensions of Xcc mixed with HN-2 wild-type (6 × 10 8 CFU·mL −1 ), and suspensions of Xcc mixed with fadT in-frame ∆fadT deletion mutant (6 × 10 8 CFU·mL −1 ). Radish root slices inoculated only with distilled water were used as the control. Three replicates of each treatment were set, and the experiments were carried out at least thrice. The radish root slices were incubated at 30 • C for 2 days in an incubator. Daily records of symptoms were taken after bacterial inoculation. The macerated area was measured to evaluate the disease severity [15,51].
For complementation of ∆fadT, the coding sequence of fadT and its indigenous promoter sequence were amplified by PCR and cloned into HindHIII/BamHI sites of pBBR1MCS-5, generating plasmid pBBR1MCS-5-fadT. pBBR1MCS5-fadT was mobilized into ∆fadT mutant by triparental conjugal mating as above. The transformants were selected on LB medium containing gentamicin and chloramphenicol. Correct transformants were confirmed by PCR and DNA sequencing. The primers used for cloning and detection are all listed in Table 2. Table 2. Primers used in this study.

Purification of FadT
Enzyme purification was performed according to the methods described in previous reports [52,53] with modifications. Cell cultures (1 L) were separated via centrifugation at 8000× g for 10 min. The cell pellets were resuspended in 40 mL binding buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH 7.0) and subjected to 400 pulses of sonication (400 W, 3 s each with a 5 s interval) in an ice-water bath [54]. Following centrifugation (13,000× g) at 4 • C for 30 min, the supernatant was passed through a 0.22 µm filter and applied to a GSTrap (GE Healthcare, Marlborough, MA, USA) FF column of the liquid chromatography system, AKTAPure (GE Healthcare, Marlborough, MA, USA), and GST fusion proteins were eluted via a GSTrap FF column using 10 mM reduced glutathione according to standard affinity chromatography. GST fusion proteins were digested using PreScission protease (GE Healthcare, Marlborough, MA, USA) according to the manufacturer's instructions.

Enzyme Activity Assay
FadT enzyme activity was investigated using a 0.5 mL reaction mixture containing 2 mM DSF, 100 mM sodium phosphate buffer (pH 7.0), and an appropriately diluted FadT preparation. After 30 min of incubation at 35 • C, the reaction was stopped by adding 100 µL of 1 M trichloroacetic acid [55]. Concentrations of DSF residues in the reaction mixture were extracted using ethyl acetate and detected via high-performance liquid chromatography (HPLC) (Waters 2690, Milford, MA, USA) according to the method described by Ye et al. [15]. Each treatment was performed in triplicate with inactivated enzyme as control. One unit of enzyme activity (U) was defined as the amount required to catalyze the formation of 1 µmol of product per minute [52,53].

Virulence Tests
The virulence of the Xanthomonas campestris pv. campestris strain on host plants was tested by the leaf-clipping method as previously described [56,57]. Healthy Chinese cabbage (Brassica pekinensis (Lour.) Rupr.), radish (Raphanus sativus L.), and pakchoi (Brassica campestris L. ssp. chinensis Makino) seeds were cultivated and acclimated in sterile soil under irregular irrigation for 30 days with no application of pesticides. These plants grew in flower pots in corridors and under a ceiling to block some of the rain. The day/night temperature, humidity, and photoperiod were all set to match the natural environment during the experiment. The experimental design included the following five treatments: (1) distilled water, which was used as the control; (2) Xcc at 6 × 10 8 CFU·mL −1 ; (3) Xcc and ∆fadT at 6 × 10 8 CFU·mL −1 ; (4) Xcc and ∆fadT (fadT) at 6 × 10 8 CFU·mL −1 ; (5) Xcc (fadT) at 6 × 10 8 CFU·mL −1 . Three replicates of each treatment were used, and the experiments were carried out at least thrice. Daily records of symptoms were taken after bacterial inoculation [51,58]. The details of the tests are as follows: The strains used in this part were already cultured in 5 mL LB medium and shaken at 30 • C and 200 rpm for 12-16 h. Then, 100 µL of bacterial suspensions were inoculated on each plant. Next, Chinese cabbage and radish were harvested after 8 days, and pakchoi was harvested after 10 days. The macerated tissue was measured to evaluate the disease severity. The percentage of maceration was calculated by comparison with the preinoculation tissue [15,51].

GenBank Accession Number
The 16S rDNA gene sequences of C. pinatubonensis HN-2 were deposited into the NCBI nucleotide sequence database under the accession number MG561941.1. The wholegenome sequence of HN-2 and sequence of fadT were also deposited in the NCBI database with accession numbers CP080766-CP080769 and MZ734614, respectively. The genome annotation and all annotated protein sequences of HN-2 were deposited in Figshare (https://figshare.com/s/eee14ba9310748460cf8, accessed on 22 August 2021).

Sequencing and Analysis of the C. pinatubonensis HN-2 Genome
The whole genome of C. pinatubonensis HN-2 was sequenced by both Illumina shortread and Oxford Nanopore long-read sequencing technologies, generating 4.29 and 1.63 Gb of sequencing data, respectively. A hybrid de novo assembly using both types of sequencing data resulted in four circular replicons with a total size of 7.55 Mb and a GC-content of 64.29%. The C. pinatubonensis HN-2 genome assembly consists of two chromosomes (Chr1: 3.89 Mb; Chr2: 2.38 Mb) and two plasmids (Plas1: 689.0 kb; Plas2: 237.7 kb) ( Figure 1). BUSCO assessment of the C. pinatubonensis HN-2 genome assembly showed that all the 688 Burkholderiales-wide single-copy orthologs were detected as complete genes, indicating that its quality was superior. nucleotide identity (ANI) values between strain HN-2 and its close relatives in the tree. As a result, strain HN-2 was discovered to be the most closely related to C. pinatubonensis JMP134 (Figure 2), with an ANI value of 99.43%, much over the usually recognized prokaryotic species delimitation threshold of 95%. Therefore, we assigned strain HN-2 as a strain of C. pinatubonensis based on genome data analysis.  In total, 7101 protein-coding genes, 65 tRNA genes, 18 rRNA genes, and 1 small RNA gene were predicted in HN-2. The protein-coding genes have a total length of 6,556,812 bp, accounting for 86.86% of the genome size, and an average length of 923 bp (Table S1). Table S2 contains the findings of the functional annotation of protein-coding genes and the prediction of additional genomic characteristics. There were 14 gene islands with total and average lengths of 183,507 and 13,108 bp, respectively, and the statistical map of gene distribution in the gene island is shown in Figure S1. There were also four preprophages with total and average lengths of 269,803 and 67,450.8 bp, respectively.
There were 6747, 655, 5451, 4681, 2669, and 589 coding genes annotated by NR, KEGG, COG, GO, SwissProt, TCDB, PHI, and CAZy. From the GO database, 2544 complete CDSs (coding sequences) were obtained from the coding region prediction of the strain HN-2 genome, accounting for 89.23% of the total genome. There were 2512 genes involved in the metabolic process, representing the largest number of genes, followed by 2326 and 2247 genes involved in the cellular process and catalytic activity, respectively. Figure S2 presents the classification of HN-2 functional genes in the GO database. The COG database functionally classifies 3299 CDSs, accounting for 88.99% of the total number of coding genes, and Figure S3 presents the classification of HN-2 functional genes in the COG database. There were a total of 671 genes belonging to general predicted (R) functions that were not explicitly labeled to a specific group among these functional genes. Next, there were 623, 599, 513, and 499 genes with the functions of energy production and conversion (C), transcription (K), amino acid transport and metabolism (E), and lipid transport and metabolism (I), respectively. The above two databases provide a clearer view of the relevant functional properties of strain HN-2, in which genes with functions in metabolism and catalytic activity are relatively abundant, providing references for subsequent studies on genes that may be closely related to the function of strain HN-2 in DSF degradation. Based on the KEGG database, 223 genes were predicted as xenobiotic biodegradation and metabolism genes. According to the comparison of the database, the quantity and species of biodegradation metabolism pathways were abundant in the genome of strain HN-2, and this result was mutually confirmed with the results annotated by the GO and COG databases. Other KEGG pathway annotations are enumerated in Figure S4. The predicted genes were also annotated against the CAZy database, and the classification annotation results, statistical tables, and individual statistics plots are displayed in Figure S5.
Our previous 16S rRNA-based analysis classified strain HN-2 as a member of the Cupriavidus genus. To further determine the taxonomic assignment of strain HN-2, we analyzed its genome sequence using GTDB-Tk, which first placed strain HN2 into a prebuilt bacterial phylogeny based on a set of 120 proteins and then calculated average nucleotide identity (ANI) values between strain HN-2 and its close relatives in the tree. As a result, strain HN-2 was discovered to be the most closely related to C. pinatubonensis JMP134 (Figure 2), with an ANI value of 99.43%, much over the usually recognized prokaryotic species delimitation threshold of 95%. Therefore, we assigned strain HN-2 as a strain of C. pinatubonensis based on genome data analysis.

Identification and Cloning of the Gene Responsible for the DSF Degradation
DSF is an unsaturated fatty acid, and, therefore, the ability of the strain HN-2 to quench DSF-mediated QS probably depends on enzymes in the fatty acid degradation pathway. For identification of genes in charge of DSF degradation in strain HN-2, we selected eight genes for further investigation based on their functional annotations (domain structures of their protein products are shown in Figure S6); these genes encode four longchain acyl-CoA synthetases (HN.2_GM000231, HN.2_GM003041, HN.2_GM005970, and HN.2_GM000743, which were annotated with the KO term K01897 and are also homologs of rpfB), three 3-hydroxyacyl-CoA dehydrogenases (HN.2_GM000404, HN.2_GM003236, and HN.2_GM004234, which were annotated with the KO term K07516), and one acyl-CoA dehydrogenase (HN.2_GM004237). To investigate whether these genes affected the degradation of DSF in strain HN-2, mutants with deletion of these genes were generated using strain HN-2 as the parental strain. Finally, the 0404 in-frame ∆0404 deletion mutant, the 3236 in-frame ∆3236 deletion mutant, the 4234 in-frame ∆4234 deletion mutant, the 4237 in-frame ∆4237 deletion mutant, the 0231 in-frame ∆0231 deletion mutant, the 3041 in-frame ∆3041 deletion mutant, the 5970 in-frame ∆5970 deletion mutant, and the 0743 in-frame ∆0743 deletion mutant were generated. After exploring their inhibitory effect on the pathogenicity of Xcc, GM004237 was initially identified as a DSFdegradation gene in strain HN-2 and named fadT. The other genes GM000231, GM003041,

Identification and Cloning of the Gene Responsible for the DSF Degradation
DSF is an unsaturated fatty acid, and, therefore, the ability of the strain HN-2 to quench DSF-mediated QS probably depends on enzymes in the fatty acid degradation pathway. For identification of genes in charge of DSF degradation in strain HN-2, we selected eight genes for further investigation based on their functional annotations (domain structures of their protein products are shown in Figure S6); these genes encode four long-chain acyl-CoA synthetases (HN.2_GM000231, HN.2_GM003041,  HN.2_GM005970, and HN.2_GM000743, which were annotated with the KO term K01897 and are also homologs of rpfB), three 3-hydroxyacyl-CoA dehydrogenases (HN.2_GM000404, HN.2_GM003236, and HN.2_GM004234, which were annotated with the KO term K07516), and one acyl-CoA dehydrogenase (HN.2_GM004237). To investigate whether these genes affected the degradation of DSF in strain HN-2, mutants with deletion of these genes were generated using strain HN-2 as the parental strain. Finally, the 0404 in-frame ∆0404 deletion mutant, the 3236 in-frame ∆3236 deletion mutant, the 4234 in-frame ∆4234 deletion mutant, the 4237 in-frame ∆4237 deletion mutant, the 0231 in-frame ∆0231 deletion mutant, the 3041 in-frame ∆3041 deletion mutant, the 5970 The biocontrol experiment showed that strain HN-2 could well decrease DSF-mediated Xcc pathogenicity, whereas the fadT in-frame ∆fadT deletion mutant had no inhibitory effect on DSF-mediated Xcc pathogenicity. Biocontrol efficacy against black rot in Chinese radish of the control (CK), wild-type (strain HN-2), and ∆fadT is shown in Figure 3. It is worth pointing out that the wild-type strain HN-2 exhibited superior DSF degradation activity and completely degraded 2 mM of DSF within 24 h. In addition, ∆fadY could not degrade DSF, and ∆fadT (fadT) regained the ability to degrade DSF, which further indicated that fadT played a key role in the degradation of DSF. The comparative study of DSF-degrading activity between the control (CK), wild-type strain HN-2, ∆fadT, and ∆fadT (fadT) cultured in MSM medium for 24 h is shown in Figure S7. These results identified fadT as the key gene affecting wild HN-2 in the alleviation of bacterial virulence on host plants, and it was thus identified as an important gene responsible for DSF degradation. degradation activity and completely degraded 2 mM of DSF within 24 h. In addition, ∆fadY could not degrade DSF, and ∆fadT (fadT) regained the ability to degrade DSF, which further indicated that fadT played a key role in the degradation of DSF. The comparative study of DSF-degrading activity between the control (CK), wild-type strain HN-2, ∆fadT, and ∆fadT (fadT) cultured in MSM medium for 24 h is shown in Figure S7. These results identified fadT as the key gene affecting wild HN-2 in the alleviation of bacterial virulence on host plants, and it was thus identified as an important gene responsible for DSF degradation.

Codon Optimization, Expression, and Purification of FadT
In order to obtain a higher yield and a pure DSF-degrading enzyme, the gene fadT was codon optimized. After the optimized gene co-fadT was obtained, it was synthesized and ligated into the vector pGEX-6p-1. In addition, pGEX-6p-1-co-fadT was obtained and then transferred into E. coli BL21. Finally, the engineered strain E. coil BL21/pGEX fadT was acquired, and it was abundantly expressed to obtain the crude enzyme extract, followed by affinity purification. As shown in Figure S8, the SDS-PAGE staining showed

Codon Optimization, Expression, and Purification of FadT
In order to obtain a higher yield and a pure DSF-degrading enzyme, the gene fadT was codon optimized. After the optimized gene co-fadT was obtained, it was synthesized and ligated into the vector pGEX-6p-1. In addition, pGEX-6p-1-co-fadT was obtained and then transferred into E. coli BL21. Finally, the engineered strain E. coil BL21/pGEX fadT was acquired, and it was abundantly expressed to obtain the crude enzyme extract, followed by affinity purification. As shown in Figure S8, the SDS-PAGE staining showed that GST-FadT had a molecular weight of about 93 kDa as predicted in silico. The band of GST-FadT was single, indicating the enzymes were of high purity. The purified FadT protein exhibited high enzymatic activity and outstanding stability over a broad pH (6.0-8.0) and temperature (25-45 • C) range with maximal activity at pH 7.0 and 35 • C. No cofactors were required for FadT enzyme activity. Moreover, the FadT enzyme showed a strong ability to degrade DSF ( Figure S9).

Xcc Expressing fadT Loses Virulence in Planta
To explore the function of f adT gene in the biocontrol of Xcc, we performed plant inoculation experiments. Radish (Raphanus sativus L.), Chinese cabbage (Brassica pekinensis (Lour.) Rupr.), and pakchoi (Brassica campestris L. ssp. chinensis Makino), commonly used in the test of biocontrol of black rot disease, were inoculated with Xcc together with wild-type or f adT-deletion strain HN-2. Each tested plant inoculated with Xcc alone presented severe black rot symptoms (Figures 4-6

Discussion
Understanding the genomic diversity of bacterial strain and the relevant bioactive products are of importance for disease control [59,60]. In this study, whole-genome sequencing (WGS) combined with genome annotation was applied to uncover the mechanism of DSF degradation in Cupriavidus pinatubonensis strain HN-2. The genome sequence of strain HN-2 discovered in this study will be useful in the development of

Discussion
Understanding the genomic diversity of bacterial strain and the relevant bioactive products are of importance for disease control [59,60]. In this study, whole-genome sequencing (WGS) combined with genome annotation was applied to uncover the mechanism of DSF degradation in Cupriavidus pinatubonensis strain HN-2. The genome sequence of strain HN-2 discovered in this study will be useful in the development of DSF-degrading bacteria or enzymes in the future. Further studies require a focus on genes related to the pathway that metabolizes DSF.
The emergence of antibiotic-resistant bacteria causes more difficulties in the treatment of bacterial infectious diseases. As for the control of diseases caused by bacteria, the use of QQ is a promising avenue to replace traditional antibiotics. In principle, QQ quenches the QS molecules of targeted bacteria, resulting in the shutdown of the expression of QS-controlled virulence genes [61][62][63][64]. As a result, a variety of bacteria that degrade DSF have been found and characterized, including Staphylococcus, Bacillus, Microbacterium, Paenibacillus, Cupriavidus, Acinetobacter, and Pseudomonas [15,[65][66][67][68][69]. However, the genome sequence and molecular mechanisms of DSF-degrading strains are not clear, which limits the applications of these QQ strains. Since the discovery of the first AHL lactase AiiA isolated from Bacillus cereus [66] and the AHL amidase isolated from Variovorax paradoxus, an increasing amount of AHL-degrading enzymes have been well identified [70][71][72][73][74].
DSF has multiple effects on various cellular processes of bacteria. It has been reported that DSF can block protease secretion from attack-phase cells of Bdellovibrio bacteriovorus 109 J completely at the concentration of 50 mM and negatively regulate the membrane integrity at a concentration of 200 mM [75]. Additionally, DSF inhibits biofilm formation with no impacts on the growth of P. aeruginosa under static and continuous conditions [76]. DSF also regulates intercellular public goods (such as extracellular polymeric substances (EPS) and amino acids) [76]. DSF-based QS systems are classified into three major classes, the first from Xcc, the second from Burkholderia cenocepacia and Cronobacter turicensis, and the third from Pseudomonas aeruginosa [77][78][79][80]. However, limited DSF-degrading bacteria and their corresponding enzymes were identified. This is the first research that we are aware of that describes a novel DSF-degrading enzyme in C. pinatubonensis.
Our findings show that expressing the fadT gene in Xcc significantly reduced black rot symptoms in the tested host plants, indicating FadT as a novel DSF-degrading enzyme with significant efficacy in reducing Xcc pathogenicity. Our study highlights the potential use of FadT in the biocontrol of Xcc and, as a starting point, indicates the significance of the application of FadT in a broader range of DSF-dependent bacterial pathogens.

Conclusions
In summary, our study first reports the genome sequence and identifies a novel DSFdegrading enzyme, FadT, in C. pinatubonensis HN-2. Plant experiments showed that Xcc carrying a functioning FadT markedly suppressed its virulence, resulting in the elimination of the symptoms of black rot in the assayed host plants. Our study demonstrates for the first time that FadT is a powerful tool for the biocontrol of black rot caused by Xcc. These novel findings reveal the biochemical characteristics of a highly efficient DSF-degrading bacterial isolate and provide useful enzymes/genes with great potential for the control of infectious diseases caused by DSF-dependent bacterial pathogens.