Inhibition of BET Proteins during Adolescence Affects Prefrontal Cortical Development: Relevance to Schizophrenia

Background: The present study investigated the role of proteins from the bromodomain and extra-terminal (BET) family in schizophrenia-like abnormalities in a neurodevelopmental model of schizophrenia induced by prenatal methylazoxymethanol (MAM) administration (MAM-E17). Methods: An inhibitor of BET proteins, JQ1, was administered during adolescence on postnatal days (P) 23–P29, and behavioural responses (sensorimotor gating, recognition memory) and prefrontal cortical (mPFC) function (long-term potentiation (LTP), molecular and proteomic analyses) studies were performed in adult males and females. Results: Deficits in sensorimotor gating and recognition memory were observed only in MAM-treated males. However, adolescent JQ1 treatment affected animals of both sexes in the control but not MAM-treated groups and reduced behavioural responses in both sexes. An electrophysiological study showed LTP impairments only in male MAM-treated animals, and JQ1 did not affect LTP in the mPFC. In contrast, MAM did not affect activity-dependent gene expression, but JQ1 altered gene expression in both sexes. A proteomic study revealed alterations in MAM-treated groups mainly in males, while JQ1 affected both sexes. Conclusions: MAM-induced schizophrenia-like abnormalities were observed only in males, while adolescent JQ1 treatment affected memory recognition and altered the molecular and proteomic landscape in the mPFC of both sexes. Thus, transient adolescent inhibition of the BET family might prompt permanent alterations in the mPFC.


Introduction
Schizophrenia is a neurodevelopmental disorder whose first symptoms are usually diagnosed in late adolescence or early adulthood [1]. However, structural and functional abnormalities observed in individuals with schizophrenia are considered the result of early-life developmental disruption, and the neuromaturational process during adolescence appears to be important for the clinical aspects of the illness (reviewed in [1,2]). The latter observation is supported by several findings indicating that environmental or pharmacological interventions during childhood or adolescence might alter the course of schizophrenia (reviewed in [3,4]). The apparent lack of consistently replicated major genetic defects and the increasing evidence of changes in gene expression after exposure to environmental risk factors suggest that epigenetic regulation might be involved in the aetiology of schizophrenia (reviewed in [5]). However, limited data related to epigenetic regulation, especially in adolescence, of alterations in the developmental trajectory of the brain predisposed genetically or environmentally to schizophrenia symptom appearance are available.
The histone acetylation pattern is one of the epigenetic modifications that might be critical to gene transcription and involved in the brain maturation process [6]. Moreover, 2 of 29 previous data from our [7] and other [8] studies using animal neurodevelopmental models of schizophrenia indicated that the regulation of histone acetylation in the medial prefrontal cortex (mPFC) during early adolescence might be an important mechanism involved in the development of schizophrenia-like abnormalities in adulthood. The pattern of acetylation marks is recognised by acetyl-lysine residues such as bromodomain (epigenetic readers) functioning as a scaffold for assembly of macromolecular complexes that alter chromatin accessibility to transcription factors and allow the recruitment or activation of RNA polymerases [9]. These complexes then initiate transcriptional programmes that result in altered gene expression. Epigenetic readers of acetylation marks from the bromodomain and extra-terminal domain (BET) family have been shown to be key regulators of chromatin dynamics and the disease-associated acetylome [9,10]. The BET family is comprised of four proteins: BRD2, BRD3, and BRD4, which are expressed in most cells and tissues, and BRDT that is primarily localised in the testes [11]. Acetyl-lysine mimic compounds, namely, BET inhibitors (JQ1, I-BET, and MS417) with high affinity and specificity to BET bromodomains, function by blocking BET proteins from binding acetylated chromatin. Compounds regulating the function of BET proteins might alter epigenetic status and affect transcriptional activity in cells [9,10].
Some evidence indicates the involvement of BET proteins in neuroplasticity regulation and memory formation, as well as their potential roles in the pathomechanisms of neurodevelopmental disorders [12][13][14]. Findings from animal studies showed a selective upregulation of BRD4 protein in an animal model of Fragile X Syndrome (FXS), a neurodevelopmental disorder that might cause intellectual disability or autism spectrum disorder [13]. An inhibition of BRD4 function diminished transcriptional disruption and reversed behavioural deficits observed in an FXS animal model [13]. On the other hand, pharmacological suppression of BET protein during mice adolescence led to selective repression of neuronal gene expression and development of autism-like syndrome [15]. However, BET protein involvement in pathogenesis of other neurodevelopmental disorders, i.e., schizophrenia, remains unknown. Therefore, in the present study, we investigated the role of the BET protein family in early adolescence, the period of proper neurodevelopment and emergence of schizophrenia-like abnormalities, in a neurodevelopmental animal model of schizophrenia induced by prenatal administration of an antimitotic agent, methylazoxymethanol (MAM), at embryonic day 17 (MAM-E17 model) [16]. We determined whether the administration of the BET family inhibitor JQ1 during early adolescence (postnatal day (P) 23-P29) may affect the behavioural response in adult rats and mPFC neurodevelopment. Our previous study using the MAM-E17 model showed that the chosen period is a critical window for epigenetically driven mPFC maturation and the development of behavioural and neurochemical schizophrenia-like abnormalities in adult MAM-E17 rats [7,17,18]. Therefore, in the present study, we analysed behavioural responses related to cognitive function: sensorimotor gating [19,20] and recognition memory [21,22]. An electrophysiological study measuring long-term potentiation (LTP) was also performed to determine adult mPFC function at the cellular level. Moreover, the expression of genes in the BET family (BRD2, BRD3, and BRD4) was measured to verify an effect of adolescent BET protein inhibition on the function of the BET family in the adult mPFC. In addition, neuronal activation in the mPFC was examined by measuring the expression of immediate early genes (IEGs: c-Fos, activity-regulated cytoskeletal (Arc), and neuronal PAS domain protein 4 (Npas4)) [23] that might be regulated by BET proteins [12].
The cortical dysfunction of GABAergic transmission observed in the prefrontal cortex of individuals with schizophrenia might result from developmental abnormalities [24], which was also confirmed in our previous study showing impaired transcription of genes that are markers of the GABA system, such as glutamic acid decarboxylase (GAD)67 and parvalbumin (PV), in the adult mPFC of MAM-E17 animals [17,25]. Thus, we verified that the expression of GAD67 and PV genes in the adult mPFC was related to dysfunction of BET proteins during adolescence. In addition to investigating the functional and molecular aspects of adolescent inhibition of BET proteins in the adult mPFC, we also designed a proteomic study to analyse the pattern of protein alterations in the adult mPFC induced by adolescent disruption of BET protein function.
The experiments were designed to assess both sexes (males and females) because of findings showing sex differences in psychiatric disorders, i.e., schizophrenia [26]. Moreover, in the MAM model of schizophrenia, different results related to the development of schizophrenia-like abnormalities are observed in males and females [27][28][29]. Thus, in our study, we also investigated whether adolescent inhibition of the BET family might exert a sex-dependent effect on the development of schizophrenia-like dysfunction.

Behavioural Response
We studied recognition memory and sensorimotor gating in both males and females to determine whether adolescent BET protein inhibition affected cognitive function in MAM-E17 rats.

Recognition Memory in Males
MAM treatment did not induce any significant differences in the time spent exploring two identical objects presented during the training session (prenatal factor × object: F (1, 44) = 3.78, p = 0.06), and no significant differences were observed between groups (prenatal × postnatal factors × object: F (1, 44) = 0.27; p = 0.61; Figure 1A). that the expression of GAD67 and PV genes in the adult mPFC was related to dysfunction of BET proteins during adolescence. In addition to investigating the functional and molecular aspects of adolescent inhibition of BET proteins in the adult mPFC, we also designed a proteomic study to analyse the pattern of protein alterations in the adult mPFC induced by adolescent disruption of BET protein function. The experiments were designed to assess both sexes (males and females) because of findings showing sex differences in psychiatric disorders, i.e., schizophrenia [26]. Moreover, in the MAM model of schizophrenia, different results related to the development of schizophrenia-like abnormalities are observed in males and females [27][28][29]. Thus, in our study, we also investigated whether adolescent inhibition of the BET family might exert a sex-dependent effect on the development of schizophrenia-like dysfunction.

Behavioural Response
We studied recognition memory and sensorimotor gating in both males and females to determine whether adolescent BET protein inhibition affected cognitive function in MAM-E17 rats.

Recognition Memory in Males
MAM treatment did not induce any significant differences in the time spent exploring two identical objects presented during the training session (prenatal factor × object: F (1, 44) = 3.78, p = 0.06), and no significant differences were observed between groups (prenatal × postnatal factors × object: F (1, 44) = 0.27; p = 0.61; Figure 1A). in the retention test conducted 1 h following the acquisition session, (C,F) discrimination index. The rats were (males: A-C and females: D-F) exposed to JQ1 or vehicle (CON) in early adolescence (P23-P29), and the analyses were performed in rats at P60. Each data point represents the mean ± SEM; n = 12 per group. B-* p textless 0.05 vs. familiar object (A), C-* p textless 0.05 vs. VEH-CON, # p textless 0.05 vs. MAM-CON (two-way repeated measures ANOVA (A,B,D,E) or two-way ANOVA (C,F) followed by a Tukey test).
However, in the retention session, MAM exerted statistically significant effects on discrimination of the novel object from the familiar object (prenatal factor × object: F (1, 44) = 14.93; p textless 0.0004), and an interaction between MAM administration and adolescent JQ1 treatment was observed (prenatal × postnatal factors × object: F (1, 44) = 51.13; p textless 0.000001). VEH-CON rats spent significantly more time exploring the novel object than the familiar object (p textless 0.003, Figure 1B). However, MAM-CON and MAM-JQ1 rats did not discriminate between the objects and spent similar amounts of time exploring each object (p = 0.065, Figure 1B). Adolescent JQ1 administration affected the exploration of the novel object by rats in the VEH group, and these animals did not discriminate between the objects and spent similar amounts of time exploring each object (p = 0.99, Figure 1B).
A significant effect of MAM treatment on the discrimination index was observed (prenatal factor: F (1, 44) = 7.7; p textless 0.009), and a significant interaction between MAM and JQ1 was identified (prenatal × postnatal factors: F (1, 44) = 27.44; p textless 0.000005). A similar decrease in the discrimination index was observed in all groups when compared to the VEH-CON group (p textless 0.0002); however, the strongest effect was observed in the VEH-JQ1 group ( Figure 1C).

Recognition Memory in Females
MAM treatment did not induce any significant differences in time spent exploring two identical objects presented during the training session (prenatal factor × object: F (1, 44) = 0.73; p = 0.4), and significant differences were not observed between groups (prenatal × postnatal factors × object: F (1, 44) = 0.14; p = 0.71; Figure 1D).
In the retention session, MAM did not affect the discrimination of the novel object from the familiar object (prenatal factor × object: F (1, 44) = 0.72; p = 0.4); however, an interaction between MAM administration and adolescent JQ1 treatment was observed (prenatal × postnatal factors × object: F (1, 44) = 17.23; p textless 0.0002). All groups spent significantly more time exploring the novel object than the familiar object (p textless 0.0002 for the VEH-CON group, p textless 0.0006 for the MAM-CON group, p textless 0.02 for the VEH-JQ1 group, and p textless 0.0002 for the MAM-JQ1 group; Figure 1E).
MAM also did not alter the discrimination index (prenatal factor: F (1, 44) = 0.68; p = 0.41); however, an interaction between MAM administration and adolescent JQ1 treatment was observed (prenatal × postnatal factors: F (1, 44) = 14.69; p textless 0.0005). MAM alone did not change the discrimination index (p = 0.18 compared with the VEH-CON group). However, JQ1 decreased the discrimination index in the VEH group (p textless 0.002 compared with the VEH-CON group and p textless 0.02 compared with the MAM-JQ1 group), and that effect was not observed in the MAM-JQ1 group (p = 0.91 compared with the VEH-CON group, Figure 1F).
The results showed a deficit in recognition memory only in the female VEH-JQ1 group.
The results revealed that either MAM or JQ1 administration affected recognition memory in males.
MAM induced a statistically significant decrease in PPI only at the lowest pre-pulse intensity used (p < 0.04 at 70 dB compared with the VEH-CON group). JQ1 administration decreased PPI in the VEH group at all applied pre-pulse intensities (p < 0.03 at 70 dB, p < 0.05 at 75 dB, and p < 0.005 at 80 dB for the comparison of the VEH-CON group with the appropriate group); however, JQ1 did not alter PPI in the MAM-treated group ( Figure  2A).
The results revealed a deficit in sensorimotor gating in all analysed male groups, however the strongest effect was observed in the VEH-JQ1 group. MAM induced a statistically significant decrease in PPI only at the lowest pre-pulse intensity used (p textless 0.04 at 70 dB compared with the VEH-CON group). JQ1 administration decreased PPI in the VEH group at all applied pre-pulse intensities (p textless 0.03 at 70 dB, p textless 0.05 at 75 dB, and p textless 0.005 at 80 dB for the comparison of the VEH-CON group with the appropriate group); however, JQ1 did not alter PPI in the MAM-treated group ( Figure 2A).
The results revealed a deficit in sensorimotor gating in all analysed male groups, however the strongest effect was observed in the VEH-JQ1 group.
Neither MAM nor JQ1 administration affected sensorimotor gating in females, however adolescent JQ1 treatment increased the average startle amplitude in both the VEHand MAM-treated groups.

Electrophysiological Recordings in the Adult Medial Prefrontal Cortex
We studied long-term potentiation in both males and females to investigate whether prenatal MAM administration and/or adolescent exposure to JQ1 affects functional synaptic plasticity in the mPFC.
An analysis of the magnitude of potentiation revealed that the MAM groups generally displayed a smaller potentiation than the VEH-CON rats (p textless 0.006 for the MAM-CON group and p textless 0.00005 for the MAM-JQ1 group). However, no differences in LTP were observed in VEH-JQ1 rats (p = 0.24 compared with the VEH-CON group, Figure 3). The rats were exposed to JQ1 or vehicle (CON) in early adolescence (P23-P29), and the analyses were performed in rats at P60-P110. The data are presented as group mean ± SEM, n = 12-17 (the number of brain sections from 6 to 9 animals). The data are expressed as percentage of the baseline amplitude values for each time point (A-C), C-the average of LTP during the last 15 min of recording. * p < 0.05 compared with the last 15 min of pre-tetanisation recording (for values averaged over the last 15 min of recording); # p < 0.05 compared with VEH-CON animals (for values averaged over the last 15 min of recording); two-way repeated measures ANOVA (A,B) or two-way ANOVA (C) followed by a Tukey test. Arrows indicate time of delivery of tetanic stimulation.

BET Family Genes
We analysed the mRNA levels of BRD2, BRD3, and BRD4 in the mPFC of both males and females to determine whether adolescent JQ1 administration affected the transcription of the BET family.  Figure 5A). The rats were exposed to JQ1 in early adolescence (P23-P29) or vehicle (CON), and the analyses were performed in rats at P60-P110. The data are presented as group mean ± SEM, n = 12-17 (the number of brain sections from 6 to 9 animals). The data are expressed as percentage of the baseline amplitude values Neither prenatal MAM nor postnatal JQ1 administration affected LTP formation in the female adult mPFC.

BET Family Genes
We analysed the mRNA levels of BRD2, BRD3, and BRD4 in the mPFC of both males and females to determine whether adolescent JQ1 administration affected the transcription of the BET family. The rats (males: A-C and females: D-F) were exposed to JQ1 or vehicle (CON) in early adolescence (P23-P29), and the analyses were performed in rats at P110. (A-C) BRD2, BRD3, and BRD4 expression in males, respectively. (D-F) BRD2, BRD3, and BRD4 expression in females, respectively. The data are expressed as percentage of VEH-CON. Each data point represents the mean ± SEM; n = 6 per group; * p < 0.05 vs. VEH-CON, # p < 0.05 vs. MAM-CON (two-way ANOVA followed by a Tukey test).
The lack of an effect of MAM on BRD2 expression was also observed in the female mPFC (prenatal factor: F (1, 20) = 0.04; p = 0.84), and no interaction was observed between the two analysed factors (prenatal × postnatal factors: F (1, 20) = 1.28; p = 0.27, Figure 5D). A decrease in BRD4 mRNA levels was observed in the MAM-CON group (p < 0.001 compared with the VEH-CON group), and this effect was abolished by adolescent JQ1 administration (p = 0.99 compared with the VEH-CON group and p < 0.02 compared with the MAM-CON group, Figure 5C). The rats (males: A-C and females: D-F) were exposed to JQ1 or vehicle (CON) in early adolescence (P23-P29), and the analyses were performed in rats at P110. (A-C) BRD2, BRD3, and BRD4 expression in males, respectively. (D-F) BRD2, BRD3, and BRD4 expression in females, respectively. The data are expressed as percentage of VEH-CON. Each data point represents the mean ± SEM; n = 6 per group; * p textless 0.05 vs. VEH-CON, # p textless 0.05 vs. MAM-CON (two-way ANOVA followed by a Tukey test).
The lack of an effect of MAM on BRD2 expression was also observed in the female mPFC (prenatal factor: F (1, 20) = 0.04; p = 0.84), and no interaction was observed between the two analysed factors (prenatal × postnatal factors: F (1, 20) = 1.28; p = 0.27, Figure 5D). A decrease in BRD4 mRNA levels was observed in the MAM-CON group (p textless 0.001 compared with the VEH-CON group), and this effect was abolished by adolescent JQ1 administration (p = 0.99 compared with the VEH-CON group and p textless 0.02 compared with the MAM-CON group, Figure 5C).
Thus, prenatal MAM administration decreased BRD4 expression only in males, and this effect was reversed by adolescent JQ1 treatment. However, neither MAM nor JQ1 administration affected BRD2 and BRD3 expression in the adult mPFC of either sex.

Immediate Early Genes
We analysed levels of the c-Fos, Npas4, and Arc mRNAs as markers of cellular activity in the mPFC of both males and females to determine whether adolescent JQ1 administration affected neuronal and synaptic activity at the molecular level.   Figure 6F).
Thus, prenatal MAM administration did not alter expression of the analysed immediate early genes in the adult mPFC of both sexes. However, JQ1 treatment repressed transcription of c-Fos and Npas4 genes in both the VEH and MAM groups of males, but in females, the Npas4 mRNA level was decreased only in the VEH group. In contrast, JQ1 administration increased Arc expression in the MAM-treated groups of both sexes.

GABAergic Markers
The expression of the GAD67 and PV mRNAs was examined to determine whether adolescent inhibition of BET proteins affected GABAergic interneurons.
• GAD67 expression MAM administration altered GAD67 expression in the male mPFC (prenatal factor: F (1, 20) = 7.14; p < 0.02). However, JQ1 treatment did not change the level of the GAD67 Figure 6. The effects of an adolescent JQ1 administration on early gene expression (c-Fos, Arc, Npas4) in the adult medial prefrontal cortex in the MAM-E17 model of schizophrenia. The rats (males: A-C and females: D-F) were exposed to JQ1 or vehicle (CON) in early adolescence (P23-P29), and the analyses were performed in rats at P110. (A-C) c-Fos, Arc, and Npas4 expression in males, respectively. (D-F) c-Fos, Arc, and Npas4 expression in females, respectively. The data are expressed as percentage of VEH-CON. Each data point represents the mean ± SEM; n = 6 per group * p textless 0.05 vs. VEH-CON (two-way ANOVA followed by a Tukey test).
Thus, prenatal MAM administration did not alter expression of the analysed immediate early genes in the adult mPFC of both sexes. However, JQ1 treatment repressed transcription of c-Fos and Npas4 genes in both the VEH and MAM groups of males, but in females, the Npas4 mRNA level was decreased only in the VEH group. In contrast, JQ1 administration increased Arc expression in the MAM-treated groups of both sexes.

GABAergic Markers
The expression of the GAD67 and PV mRNAs was examined to determine whether adolescent inhibition of BET proteins affected GABAergic interneurons. A significant decrease in GAD67 mRNA levels was detected in the MAM-CON group (p < 0.03 compared with the VEH-CON group, Figure 7A).  Figure 7D).
Thus, the prenatal MAM factor decreased both GAD67 and PV expression only in the male mPFC of the MAM-treated group. Postnatal JQ1 administration did not affect either GAD67 or PV expression in either sex.  Figure 7D).
Thus, the prenatal MAM factor decreased both GAD67 and PV expression only in the male mPFC of the MAM-treated group. Postnatal JQ1 administration did not affect either GAD67 or PV expression in either sex.

Correlation Analysis of Gene Expression and Behavioural Response
The behavioural tests and gene expression analyses were completed on the same animals. Thus, a correlation analysis was performed to determine the associations between behavioural responses and changes in gene expression in the adult mPFC ( Figure 8).

Correlation Analysis of Gene Expression and Behavioural Response
The behavioural tests and gene expression analyses were completed on the same animals. Thus, a correlation analysis was performed to determine the associations between behavioural responses and changes in gene expression in the adult mPFC ( Figure 8). The correlation matrix constructed for sensorimotor gating results (PPIs) in males revealed a positive correlation with object recognition data (DI) ( Figure 8A). This effect was not observed in females ( Figure 8B).
In males, PPI and DI showed positive correlations with the expression of the GAD67 mRNA (Gad1 gene), but they were negatively correlated with Arc expression. In addition, the expression of the BRD3 mRNA exhibited a negative correlation with the results of the novel object recognition test. The level of the BRD2 mRNA was proportional to the expression of the c-Fos and Npas4 mRNAs, while BRD3 expression was inversely proportional to the levels of these mRNAs. In contrast, BRD3 expression was positively correlated with Arc expression. Moreover, c-Fos expression was positively correlated with Npas4 expression, while Arc levels exhibited a negative correlation with the GAD67 mRNA ( Figure 8A). Thus, deficits in recognition memory and sensorimotor gating in males appear to be related to GAD67 and Arc expression. Moreover, recognition memory formation appears to be correlated with BRD3 and immediate early genes' (c-Fos, Npas4, Arc) transcription activity.
In females, PPI negatively correlated with immediate early gene expression (c-Fos, Npas4, and Arc), and DI was negatively correlated with the level of the BRD4 mRNA. However, BRD4 expression exhibited a positive correlation with the BRD3 mRNA level. In addition, c-Fos expression was positively correlated with Npas4 and Arc gene transcription ( Figure 8B). Thus, a deficit in sensorimotor gating in females appears to be related to immediate early gene expression (c-Fos, Npas4, and Arc), while recognition memory formation is rather correlated with transcription of BRD genes (BRD4, BRD3).

Proteomic Analysis of the Adult Medial Prefrontal Cortex
A proteomic study was performed to determine whether adolescent BET protein inhibition altered the protein landscape in the adult mPFC.

Proteomic Analysis of Protein Expression in Males
iTRAQ identified 313 proteins and 1049 unique peptides. Among them, 48 proteins were identified as differentially expressed (Table 1). The correlation matrix constructed for sensorimotor gating results (PPIs) in males revealed a positive correlation with object recognition data (DI) ( Figure 8A). This effect was not observed in females ( Figure 8B).
In males, PPI and DI showed positive correlations with the expression of the GAD67 mRNA (Gad1 gene), but they were negatively correlated with Arc expression. In addition, the expression of the BRD3 mRNA exhibited a negative correlation with the results of the novel object recognition test. The level of the BRD2 mRNA was proportional to the expression of the c-Fos and Npas4 mRNAs, while BRD3 expression was inversely proportional to the levels of these mRNAs. In contrast, BRD3 expression was positively correlated with Arc expression. Moreover, c-Fos expression was positively correlated with Npas4 expression, while Arc levels exhibited a negative correlation with the GAD67 mRNA ( Figure 8A). Thus, deficits in recognition memory and sensorimotor gating in males appear to be related to GAD67 and Arc expression. Moreover, recognition memory formation appears to be correlated with BRD3 and immediate early genes' (c-Fos, Npas4, Arc) transcription activity.
In females, PPI negatively correlated with immediate early gene expression (c-Fos, Npas4, and Arc), and DI was negatively correlated with the level of the BRD4 mRNA. However, BRD4 expression exhibited a positive correlation with the BRD3 mRNA level. In addition, c-Fos expression was positively correlated with Npas4 and Arc gene transcription ( Figure 8B). Thus, a deficit in sensorimotor gating in females appears to be related to immediate early gene expression (c-Fos, Npas4, and Arc), while recognition memory formation is rather correlated with transcription of BRD genes (BRD4, BRD3).

Proteomic Analysis of the Adult Medial Prefrontal Cortex
A proteomic study was performed to determine whether adolescent BET protein inhibition altered the protein landscape in the adult mPFC.

Proteomic Analysis of Protein Expression in Males
iTRAQ identified 313 proteins and 1049 unique peptides. Among them, 48 proteins were identified as differentially expressed (Table 1). The lowest Mascot score of the identified proteins was 35, with molecular masses ranging from 2 to 532 kDa (median 48 kDa). The heatmap in Figure 9A shows the protein expression levels in the analysed groups and a functional clustering analysis of the identified proteins. Proteins with similar expression patterns have relatively shorter Euclidean distances. Hierarchical clustering analysis with the Euclidean distance of proteins was used to identify patterns of protein expression following prenatal and postnatal treatment.  PCA was conducted to identify unique components related to the interaction between both treatments: prenatal (MAM) and adolescent (JQ1). The first three components (Dim1, Dim2, Dim3) defined 70% of the total variance in our experiment ( Figure 9B). An examination of the scatter plots of the proteins' principal components revealed that the first component was linked to clusters II and III from the previous classification, namely, proteins regulated by adolescent JQ1 treatment ( Figure 9B). The second component, reflecting reciprocally affected clusters I and IV from the previous analysis, identified proteins affected by prenatal MAM administration ( Figure 9B). The third component corresponded to proteins classified in different defined clusters from a previous analysis; however, it comprised proteins functionally related to synapse and cytoskeleton organisation (AP2B1, CAZA2, DPYL2, MYPR, NEUM, SYUB, and VGLU1) or energy transfer processes (AT1A2, ATPB, KCRU, and VATL) ( Figure 9B).
The two first components (Dim1, Dim2) differentiate all experimental groups ( Figure 9C). The proteomic analysis in the male adult mPFC identified distinctive molecular signatures related to either prenatal factor (MAM administration) or postnatal factor (adolescent JQ1 treatment) action.

Proteomic Analysis of Protein Expression in Females
iTRAQ identified 348 proteins and 1190 unique peptides. Among them, 26 proteins were identified as differentially expressed (Table 2).  Table 1. The lowest Mascot score of the identified proteins was 26, with molecular masses ranging from 2 to 532 kDa (median 48 kDa). The heatmap in Figure 10A shows the protein expression levels in the analysed groups and a functional clustering analysis of the identified proteins. Proteins with similar expression patterns have relatively shorter Euclidean distances. Hierarchical clustering analysis with the Euclidean distance of proteins was used to identify patterns of protein expression following prenatal and postnatal treatment. nent, reflecting reciprocally affected clusters I and II (synaptic proteins: CLH1, DCLK1, and EAA1) from the previous analysis, identified proteins that were altered by adolescent JQ1 administration ( Figure 10B). The third component corresponded to proteins classified in cluster I in the previous analysis ( Figure 10B). The two first components (Dim1, Dim2) separate JQ1 groups from CON groups ( Figure 10C).
Thus, proteomic analysis showed that changes in protein level in the female adult mPFC were related only to postnatal factor (adolescent JQ1 treatment).  PCA was used to identify unique components related to the interaction between both treatments: prenatal MAM and adolescent JQ1. The first three components (Dim1, Dim2, Dim3) defined 70% of the total variance in our experiment ( Figure 10B). An examination of the scatter plots of the proteins' principal components revealed that the first component (Dim1) was linked to clusters I and II from the previous classification, indicating proteins regulated by adolescent JQ1 treatment ( Figure 10B). Similar to Dim1, the second component, reflecting reciprocally affected clusters I and II (synaptic proteins: CLH1, DCLK1, and EAA1) from the previous analysis, identified proteins that were altered by adolescent JQ1 administration ( Figure 10B). The third component corresponded to proteins classified in cluster I in the previous analysis ( Figure 10B). The two first components (Dim1, Dim2) separate JQ1 groups from CON groups ( Figure 10C).
Thus, proteomic analysis showed that changes in protein level in the female adult mPFC were related only to postnatal factor (adolescent JQ1 treatment).

Discussion
As shown in the present study, the administration of JQ1, an inhibitor of BET family proteins, in a defined period of adolescence affected behavioural responses and molecular and proteomic landscapes in the adult mPFC. The effects of JQ1 action were observed in animals of both sexes prenatally treated with VEH and MAM.

Behavioural Response
Behavioural tests were performed to analyse deficits in sensorimotor gating and recognition memory that are commonly observed in the MAM-E17 rat model of schizophrenia [30][31][32][33]. These behavioural impairments are considered models of the cognitive symptoms of schizophrenia. Sensorimotor gating is a pre-attentive process that is measured by PPI, and abnormalities in pre-attentive information processing may predict or lead to complex cognitive deficits [19]. PPI deficits were originally identified in patients with schizophrenia, and in preclinical studies, PPI was also used to investigate sensorimotor gating deficits in animal models of schizophrenia [19,20]. The memory impairment was examined using the novel object recognition test, which is known to have translational relevance to the domain of visual learning and memory [21,22], among the cognitive deficits observed in patients with schizophrenia [34]. The aforementioned task is a useful tool to evaluate recognition memory in animal models; however, some limitations related to the preference of a novel object should be considered, and the mechanisms involved in visual recognition memory of humans and rodents are not identical [21].
In the present study, we observed impairments in sensorimotor gating and memory recognition in male but not female MAM-E17 rats. Our previous studies also showed a decrease in PPI and memory impairments in the novel object recognition test in MAM-treated male rats [17,18,35]. However, in these studies, we did not analyse female rats. Literature data indicate some deficits in sensorimotor gating [36] or a reduction in discrimination index in novel object recognition in MAM-E17 females [29]. However, these studies were performed in Sprague-Dawley rats rather than in the Wistar Han rats used in our study. On the other hand, some clinical studies reported that healthy women showed lower levels of PPI than men [37,38], and a similar effect was reported in our study, where the PPI of males was higher than that of females. Moreover, the results from patients with schizophrenia are inconsistent, showing a decrease or unaffected sensorimotor gating in females [37,39], similar to those noted in the MAM-E17 model. Thus, female rats might not display all of the schizophrenia-like behavioural deficits induced by prenatal MAM treatment that were observed in male rats.
Adolescent JQ1 treatment affected the analysed behavioural parameters. In males, JQ1 impaired either memory recognition or sensorimotor gating in control rats (VEH-JQ1 group) but did not alter these parameters in the MAM group. In females, adolescent BET protein inhibition induced a reduction in novel object recognition in control rats; however, prenatal MAM treatment prevented this effect. On the other hand, JQ1 did not alter PPI but increased the startle amplitude in the VEH-and MAM-treated groups of female rats. To the best of our knowledge, the effect of JQ1 treatment has not yet been studied in acoustic startle response tests; thus, we are unable to easily compare our data with others. However, the role of BET proteins was determined in a novel object recognition test, and JQ1-treated adult animals exhibited impaired memory formation [12] and the blockade of an enhancement of recognition of a new object induced by an HDAC3 inhibitor [40]. Thus, our results add new knowledge of the role of BET proteins in the development of some behavioural responses, such as sensorimotor gating and recognition memory. Moreover, our data imply that the inhibition of BET family function during adolescence might disrupt these processes in adulthood, especially in males.

LTP in the Adult mPFC
The prefrontal cortex functionally and structurally transforms during the transition from adolescence to adulthood, which makes it very vulnerable to any environmental changes. Abnormal prefrontal cortical development might affect cognitive function in adulthood [41]. Thus, we analysed LTP formation in the adult mPFC to examine cortical cellular function, and we observed deficits in LTP only in the MAM-E17 male but not in the female mPFC. Our results are consistent with previous findings [27], showing a decrease in LTP only in the mPFC of MAM-treated adult male, not female mice. Thus, the available data suggest that male mPFC development might be more vulnerable to prenatal MAM treatment than female mPFC development.
Adolescent JQ1 administration only exerted a slight insignificant effect on LTP formation in the mPFC of control adult animals of both sexes and did not affect the MAM groups. On the other hand, JQ1 might significantly increase LTP in mouse hippocampal slices [42]. Thus, the role of the BET protein in LTP formation might depend on the brain region and neurodevelopmental period, and our results did not indicate sex differences in the effect of JQ1 on LTP in the mPFC.

Molecular Changes in the Adult mPFC
We examined the effect of adolescent functional blockade of BET proteins on BRD gene expression in the adult mPFC. Prenatal MAM administration decreased BRD4 expression only in male rats, and it had no effect on BRD2 and BRD3 mRNA levels in either sex. To the best of our knowledge, this report is the first to show changes in BRD expression in the mPFC, which might suggest the involvement of the BET family in prefrontal cortical maturation and the development of schizophrenia-like abnormalities in male MAM-E17 rats. Adolescent JQ1 treatment did not affect BRD expression; however, it abolished the MAM-induced decrease in BRD4 mRNA levels in the male mPFC. Thus, JQ1 administration during adolescence might normalise BRD cortical dysfunction.
In the present study, levels of IEGs (c-Fos, Arc, and Npas4) were examined as indirect markers of neuronal activity related to mPFC function. c-Fos and Npas4 are transcription factors that regulate downstream gene expression, and Arc encodes an effector protein that directly modulates cellular function [43]. c-Fos is expressed in an activity-dependent manner and is involved in complex learning and memory processes [23]. Arc is known to encode a synaptic protein that is involved in the generation of new synapses, and maintenance of the old synapses requires plasticity mechanisms (i.e., LTP) [23]. Npas4 plays an important role in adolescent maturation of the prefrontal inhibitory network [44] and regulates the excitatory and inhibitory balance at synaptic connections [45]. Thus, these IEGs are important for synaptic and neuronal plasticity, and they are considered involved in the development of psychiatric disorders (i.e., schizophrenia and autism) [23,46]. Prenatal MAM treatment did not alter the expression of any of the analysed IEGs in either sex. However, a gene-specific effect of adolescent JQ1 administration was observed. The effect of JQ1 on c-Fos expression was sex-specific, as it decreased the c-Fos mRNA level only in the male control and MAM groups. In contrast, adolescent inhibition of BET proteins induced an increase in Arc expression only in the MAM-treated group in both sexes. Npas4 transcription was also altered by JQ1 treatment, and similar to the regulation of c-Fos mRNA expression, a reduction in the level of the Npas4 mRNA was observed in male rats in the VEH and MAM groups. However, in females, a decrease in the Npas4 mRNA level was detected only in the control group. Nonetheless, correlation data showed a positive association between c-Fos and Npas4 expression in both sexes that might suggest an important role of both genes in JQ1's effect on neuronal activity in the mPFC. Recent evidence reported that JQ1 regulates c-Fos and Arc gene expression in vitro [12]. Thus, our results confirm previous findings related to c-Fos and Arc regulation, but also suggest that Npas4 transcription might be regulated by JQ1. The direction of changes in c-Fos and Npas4 expression observed in the present study were related to decreases in behavioural response (sensorimotor gating and novel object recognition) induced by JQ1 treatment in males. In females, only decreased levels of the Npas4 transcript in the mPFC might be related to an effect of JQ1 on the behavioural response of the control group (reduction in DI in the novel object recognition task and lack of effect on sensorimotor gating), as well as some slight effect on LTP observed in the female cortex. Thus, adolescent JQ1 administration modified neuronal and synaptic activity in the adult mPFC by altering expression of IEGs, and males might be more vulnerable to adolescent JQ1 treatment than females.
Our previous study showed a decrease in the mRNA levels of both GAD67 and PV induced by prenatal MAM treatment in the adult male mPFC [17,18,25]. In the present study, we also observed a decrease in GAD67 and PV expression in the mPFC of males but not females. Adolescent JQ1 treatment did not affect the expression of either GAD67 or PV in the mPFC of either sex. Thus, the dysfunction of GABAergic interneurons in the male mPFC might be involved in the deficits in sensorimotor gating, memory recognition, and LTP observed in MAM-treated males, but not female rats.
A correlation study of behavioural responses and gene expression also suggested sex-specific relationships in the association between these factors. Behavioural responses correlated with the expression of different genes in males (Gad1) and females (activitydependent genes: c-Fos and Npas4); however, Arc expression appears to be important in both sexes. The correlations between the analysed genes were also different in males and females, and the correlations between the expression of BRD genes and IEGs were stronger in males than in females. The latter observation suggests that alterations in BRD gene transcription might exert a stronger effect on IEG gene expression in males than in females. Therefore, adolescent treatment with JQ1 might exert a stronger effect on developmental abnormalities in males than females.

Proteomic Alterations in the Adult mPFC
Our results revealed functional and molecular changes in the adult mPFC of both sexes induced by both MAM and JQ1 administration, but the main changes were detected in males. Thus, we performed a proteomic study to investigate proteins that might be affected by either prenatal or postnatal treatment in both sexes.
Administration of either the prenatal risk factor MAM or JQ1 during adolescence altered protein levels in the male mPFC, and based on the direction of changes, proteins were divided into 4 categories. The first category of proteins showed decreased expression in the MAM-CON group. Two are related to synapse function (NEUM and SYUB), and one is involved in energy transfer processes (ATPD). The second category is a large group of proteins showing increased levels in the JQ1 groups (VEH-JQ1 and MAM-JQ1). They are related to synapse function and signalling pathways (CNTN1, CALM, CTNB1, AP2B1, AT1A2, NCAM1, CPLX1, KPCG, TENR, SYN2, NSF, VGLU1, GNAO, and VDAC1), cytoskeletal organisation (CAZA2, MYPR, MTAP2, GDIA, and DPYL4), metabolic processes (GLNA, PGK1, and KPYM), and energy transfer processes (MDHC, SUCA, ADT1, and ADT2). The levels of proteins in the third category were decreased in the JQ1 groups (VEH-JQ1 and MAM-JQ1), and they are mainly involved in synapse function (DCKL1 and RAP1B), cytoskeletal organisation (DPYL2, GMFB, NCALD, and ARP3), and metabolic and energy transfer processes (ENOA, ATPB, and KCRU). Levels of proteins in the fourth category were increased mainly in the MAM-CON group, and they are related to synapse function and signalling processes (AP2A2, CLH1, GABT, and KAPCB) or metabolic and energy transfer processes (CISY, SDHA VATL, VATA, and NDUBA).
Proteins identified in the female mPFC were divided mainly into 2 categories based on the effects of the JQ1 treatment. Levels of proteins in the first category were decreased in both the VEH-JQ1 and MAM-JQ1 groups, and they are related to synapse function or cytoskeletal organisation (AT1A1, TENR, EAA1, CAND1, TBB4A, CLH1, and DCLK1) or are involved in metabolic and energy transfer processes (NDUV2 and TCPB). The levels of proteins in the second category increased in the VEH-JQ1 and MAM-JQ1 groups, and they are involved in synaptic (FKB1A, CNTN1, SYN1, and VDAC1) and cytoskeletal organisation (SPTN1, DPYL4, SYUB, and SPTN2), as well as metabolic (LDHC, ENOG, PGK1, KPYM, and HINT1) and energy transfer processes (EF1A, EFTU, COX5B, and VATG2).
Our previous proteomic study of the male adult mPFC showed a MAM-induced decrease in the levels of proteins related to synapse and cytoskeletal organisation and a MAM-evoked increase in the levels of proteins related to metabolic and energy transfer processes [47]. The present study also showed a decrease in the levels of synaptic proteins in MAM-treated males, which might suggest changes in neuropil reorganisation in the adult mPFC and might explain the reduction in the adult mPFC volume of MAM-E17 males reported in our previous study [18]. In female mPFC, the levels of proteins related to synapse function and signalling pathways, as well as metabolic and energetic processes, were not changed after MAM treatment. The proteomic analysis identified 85% more altered proteins in MAM-and JQ1-treated males than in females, where only 15% of proteins were altered. Thus, MAM administration mainly altered protein levels in the male mPFC. Sex differences in the proteomic landscape of the MAM-treated adult mPFC were also observed by other researchers [27]. A proteomic study performed in the human prefrontal cortex indicated alterations in proteins in patients with schizophrenia. These proteins are related to dysfunctional metabolism, cytoskeleton-related abnormalities, and cellular connections [48]. Thus, the proteins that were altered in our study might be involved in schizophrenia-like abnormalities observed mainly in male MAM-treated rats.
The robust effect of adolescent JQ1 treatment on protein levels in the mPFC of both VEH-and MAM-treated rats was observed in synaptic and cytoskeletal proteins, as well as proteins involved in metabolic and energy transfer processes, in both sexes. The levels of synaptic proteins were altered in both sexes (CLH1, CNTN1, DCLK1, SYUB, TENR, and VDAC1); however, the TENR protein was regulated in a sex-specific manner, and CLH1 and SYUB were regulated only in females by JQ1. Our results showing changes induced by JQ1 in synaptic proteins are consistent with RNA-sequencing data showing JQ1-induced alterations in the transcription of synaptic proteins and receptors in neurons [12].

Animals and Treatment
Pregnant dams (Wistar Han rats) were obtained from an animal provider (Charles River, Sulzfeld, Germany) when the foetuses were aged embryonic day 15 (E15) and housed individually in polycarbonate cages (26.5 × 18 × 42 cm). The rats were randomly assigned to experimental groups; at E17, the pregnant females were intraperitoneally (i.p.) injected with 22 mg/kg of methylazoxymethanol acetate (MAM, MRIGlobal, Kansas City, MO, USA) or saline vehicle (0.9% NaCl) [7,17,18,35]. The offspring were weaned 21 days after birth, and males and females were used in our experiments. The rats were housed in large polycarbonate boxes (38 × 20 × 60 cm) in groups of 4 in the same sex on an artificial 12/12 h light/dark cycle (lights on at 7 A.M.) with ad libitum access to food and water. The experimental groups consisted of animals chosen randomly from different litters (total 16) to avoid litter effects (maximum 2 rats from a single litter per group). The BET inhibitor (+)JQ1 ((6S)-4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno [3,2-f ] [1,2,4]triazolo [4,3-a] [1,4]diazepine-6-acetic acid 1,1-dimethylethyl ester; STI, Poznan, Poland) was administered at a dose of 25 mg/kg i.p. once a day for 7 consecutive days in early adolescence (23rd-29th days of life). JQ1 was dissolved in 10% DMSO and then diluted in corn oil. All experiments (behavioural and biochemical) were performed on four experimental groups (VEH-CON, VEH-JQ1, MAM-CON, and MAM-JQ1) using the same set of animals, but electrophysiological studies were performed using different sets of rats ( Figure 11). The behavioural experiments were performed in adult rats as described below. Rats performed a novel object recognition test at P60 followed by an acoustic startle response test a week later. For the behavioural studies, each experimental group consisted of 12 rats per group. The animals were sacrificed a month after the last test, and brain tissue was stored until use in biochemical experiments. Biochemical studies (qRT-PCR and proteomics) were performed on the same mPFC samples obtained from adult rats at P110, and each experimental group consisted of 4-6 animals. A separate set of animals was used for the electrophysiological study performed on the mPFC of adult animals aged P60-P110, and each group consisted of 6-9 animals.  [1,4]diazepine-6-acetic acid 1,1-dimethylethyl ester; STI, Poznan, Poland) was administered at a dose of 25 mg/kg i.p. once a day for 7 consecutive days in early adolescence (23rd-29th days of life). JQ1 was dissolved in 10% DMSO and then diluted in corn oil. All experiments (behavioural and biochemical) were performed on four experimental groups (VEH-CON, VEH-JQ1, MAM-CON, and MAM-JQ1) using the same set of animals, but electrophysiological studies were performed using different sets of rats ( Figure 11). The behavioural experiments were performed in adult rats as described below. Rats performed a novel object recognition test at P60 followed by an acoustic startle response test a week later. For the behavioural studies, each experimental group consisted of 12 rats per group. The animals were sacrificed a month after the last test, and brain tissue was stored until use in biochemical experiments. Biochemical studies (qRT-PCR and proteomics) were performed on the same mPFC samples obtained from adult rats at P110, and each experimental group consisted of 4-6 animals. A separate set of animals was used for the electrophysiological study performed on the mPFC of adult animals aged P60-P110, and each group consisted of 6-9 animals. The study was carried out in strict accordance with the recommendations in the European Council Guide for the Care and Use of Laboratory Animals (86/609/EEC) and revised by Directive 2010/63/UE for the protection of animals used for scientific purposes. The protocols (permission number 138/2020; data of approval 4 June 2020) were approved by the Committee for Laboratory Animal Welfare and the Ethics of the Maj Institute of Pharmacology, Polish Academy of Sciences in Kraków, and were conducted in accordance with the current version of the Polish Law on the Protection of Animals (266/2015).

Novel Object Recognition Test
The study was performed as described previously with some modifications [17,35]. Rats were tested for memory deficits in dimly lit (18 lx) cages made of black Plexiglas (60 × 60 × 30 cm). After each measurement, the floor and walls were cleaned and dried. Twenty-four hours before training, the animals were habituated to the arena (without any objects) for 10 min. Objects used for the discrimination task included a black glass cylinder and a white plastic cuboid filled with water. The objects were placed in two opposite corners of the arena, approximately 10 cm from the walls. The rats were placed in the middle of the arena, and during the training session (acquisition), two identical objects (A and A1) were presented for 5 min (half of the animals were presented with two black glass

Novel Object Recognition Test
The study was performed as described previously with some modifications [17,35]. Rats were tested for memory deficits in dimly lit (18 lx) cages made of black Plexiglas (60 × 60 × 30 cm). After each measurement, the floor and walls were cleaned and dried. Twenty-four hours before training, the animals were habituated to the arena (without any objects) for 10 min. Objects used for the discrimination task included a black glass cylinder and a white plastic cuboid filled with water. The objects were placed in two opposite corners of the arena, approximately 10 cm from the walls. The rats were placed in the middle of the arena, and during the training session (acquisition), two identical objects (A and A1) were presented for 5 min (half of the animals were presented with two black glass cylinders, and the other half with two white plastic cuboids). After a 1 h rest in the home cage, the rats were again placed in the same Plexiglas cage as in the previous test and presented with two objects for 3 min, but one of the objects was replaced by a novel object (A-familiar and B-novel) (recognition session). The objects were cleaned with a 70% ethanol solution between sessions. Exploration was defined as sniffing the object and/or touching it with the nose or forepaws. The animal behaviour was automatically recorded by cameras (SJ8Pro SJCAM, China) and next, videos were analysed using the Solomon Coder program (https://solomon.andraspeter.com, accessed on 5 August 2021). Animals showing less than 5 s of exploration time were excluded from the experiment. Based on the time spent exploring (E) each object, a discrimination index (DI) was calculated: DI = (E B − E A )/(E A + E B ).

Acoustic Startle Response Test
The efficacy of sensorimotor gating was measured by recording pre-pulse inhibition (PPI) of the acoustic startle response, and reactivity was measured using a startle apparatus (SR-LAB, San Diego Instruments, San Diego, CA, USA), as described previously [7,17,18,35]. Briefly, after a 5 min habituation period that included playing background white noise at 65 dB, two types of acoustic stimuli were presented in a random order: an acoustic stimulus alone ((P), duration 40 ms, intensity 120 dB) and an acoustic stimulus preceded by an acoustic pre-pulse ((PP), duration 20 ms, intensities: 70, 75, and 80) applied 80 ms before the stimulus (P). During each experimental session, 20 trials of each type were presented with randomised interstimulus intervals ranging from 5 to 20 s in length. The amplitudes were averaged separately for each animal and for both types of trials (stimulus alone, P; stimulus preceded by the pre-pulse, PP). The degree of pre-pulse inhibition was calculated as the percentage of inhibition (%PPI) using the following formula: [(P − PP)/P] × 100.

Electrophysiological Study
The electrophysiological procedure has been described previously [49]. The procedure was performed on adult rats between P60 and P110. The rats from each experimental group were tested in a counterbalanced, alternating order. Rats were anesthetised with isoflurane (Aerrane, Baxter, Warsaw, Poland) and decapitated. The brain was rapidly removed and immersed in an ice-cold artificial cerebrospinal fluid (ACSF) of the following composition (in mM): 130 NaCl, 5 KCl, 2.5 CaCl 2 , 1.3 MgSO 4 , 1.25 NaH 2 PO 4 , 26 NaHCO 3 , and 10 d-glucose, and ACSF was bubbled with a mixture of 95% O 2 and 5% CO 2 (pH 7.4). A block of tissue containing the mPFC was dissected and cut into 410 µm thick sections along the coronal plane using a vibratome at 1 • C (VT 1000S, Leica, Germany). The sections were transferred into a recording chamber with a fluid-gas interface and superfused at a rate of 2 mL/min with warm (32 ± 0.5 • C)-modified ACSF containing (in mM): 130 NaCl, 2 KCl, 2.5 CaCl 2 , 1.3 MgSO 4 , 1.25 NaH 2 PO 4 , 26 NaHCO 3 , and 10 D-glucose. The recordings started 2-4 h after the sections were prepared. The field potentials were evoked using square-wave stimuli (duration 0.2 ms) applied by a constant current isolation unit (WPI, Germany) at 0.016 Hz via a bipolar concentric Pt-Ir electrode (FHC, Bowdoin, ME, USA). The electrode was placed in layer II/III of the mPFC. A recording micropipette filled with ACSF (resistance of approximately 2-4 MΩ) was placed in cortical layer V of the mPFC. The responses were amplified (500×), filtered (0.1 Hz-1.3 kHz), and acquired at a 10 kHz sampling rate (EXT 10-C amplifier, NPI, Germany) using a Micro1401 interface and Signal 2 software (CED, Cambridge, UK).
After stabilisation of the responses, stimulus/response (input-output) curves were determined individually for each section by applying square stimuli of increasing intensities (duration of 0.2 ms; range of 0-50 µA, step 5 µA; range of 50-100 µA, step 10 µA; applied at 15 s intervals). Then, the stimulation intensity was adjusted to evoke a response corresponding to 40% of the maximum amplitude. When the signals became stable over a baseline period of 15 min, LTP was induced in the mPFC with a sequence of five stimulus bursts (15 s apart). A single burst consisted of 5 individual square pulses (duration of 0.3 ms, applied at 100 Hz) that were repeated ten times (0.2 s apart). The recording then continued for an additional 90 min. The response amplitude of the individual sections was normalised to the percent change relative to the baseline values. The amount of LTP was determined by comparing the data from the baseline recording averaged over the last 15 min to the data from the post-tetanic recording averaged over the last 15 min.

Tissue Preparation
After the rats were decapitated, the brains were removed, cooled on ice, and sliced into 1 mm coronal sections using a rodent brain matrix (Ted Pella, Inc., Redding, CA, USA). The mPFC was dissected from coronal sections, frozen in liquid nitrogen, and stored at −20 • C. Simultaneous extraction of proteins and nucleic acids from the same experimental sample was performed using commercially available kits (Nucleospin RNA/Protein, Macherey-Nagel, RNAeasy, Qiagen, Wroclaw, Poland) and columns (Amicon Ultra, Millipore, Warsaw, Poland) with some modifications. RNA was extracted using the RNeasy kit according to the manufacturer's instructions (www1.qiagen.com/literature/handbooks, accessed on 30 June 2021). The flow through was collected, and proteins were extracted using a Nucleospin RNA/Protein kit according to the manufacturer's instruction (www.mn-net.com/media/ pdf, accessed on 30 June 2021) and purified with Amicon Ultra Centrifugal Filter Units (Amicon). Proteins were dissolved in Dissociation Buffer (SCIEX) and quantified using the bicinchoninic acid (BCA) Protein Assay kit (Pierce, Rockford, IL, USA) according to the manufacturer's instructions (www.piercenet.com/files/1296dh4.pdf, accessed on 30 June 2021).
The obtained mRNA and proteins were used for qRT-PCR or proteomic studies, respectively.

qRT-PCR Study
qRT-PCR was performed using the QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems, Warsaw, Poland) as previously described [25]. The gene-specific primers and probes (see details in Table 3) from the TaqMan Gene Expression Assays (Applied Biosystems) were used, and 1 µL of each sample was used for cDNA amplification. Amplification was performed using the TaqMan Universal PCR Master Mix (Applied Biosystems) under the following conditions: 50 • C for 2 min and 95 • C for 10 min, followed by 40 cycles of 95 • C for 15 s and 60 • C for 1 min. The expression of the glyceraldehyde-3-phosphate dehydrogenase (Gapdh) transcript (assay Rn99999916_s1) was quantified to control for variations in cDNA levels. All qRT-PCR experiments were performed in duplicate and included no-template controls. The cycle threshold values were calculated automatically. Relative quantification was performed using the comparative threshold (CT) method, 2−∆CT, where ∆CT = (CT, target gene − CT, reference gene). Differentially expressed proteins identified in the present study were subjected to a pathway analysis. For this purpose, the Swiss-Prot accession numbers were inserted into STRING (Search Tool for the Retrieval of Interacting Genes/Proteins) software (http://string.embl.de, accessed on 15 June 2021).

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Database searching In addition, all the identified proteins were searched using UniProtKB and the Panther Database to establish their molecular functions and interactions.

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Proteomic data depository The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD027050 and 10.6019/PXD027050.

Statistical Analysis
The results are presented as the group means ± the standard errors of the means (SEM). Statistical evaluations were performed using Statistica software and consisted of one-way or two-way repeated measures analyses of variance (ANOVAs), as indicated in the figures, followed by the Tukey post hoc test. The between-(prenatal and postnatal treatment) and within-subjects (pre-pulse intensity for the acoustic startle response test or objects for the novel object recognition test) factors were used for the ANOVAs. Differences were considered significant at p textless 0.05.
All statistical analyses of proteomics data were performed using R software version 3.6.2. Corrections for multiple testing were performed using Bonferroni's method. Proteomic data were subjected to two-way ANOVA with prenatal or postnatal treatment as factors, followed by correction for multiple testing using Bonferroni's method. Differences were considered significant at p textless 0.05.
Associations among gene expression and proteomic and behavioural data were estimated by calculating Pearson's rank-based correlation coefficients, followed by correction for multiple testing using the Benjamini-Hochberg method to decrease the false discovery rate.

Conclusions
In our study, a prenatal MAM treatment exerted a sex-specific effect on the development of schizophrenia-like abnormalities, and only males were affected. Adolescent administration of JQ1 altered behavioural responses in control animals, mainly in males, but not in rats of both sexes in MAM-treated groups. Deficits in LTP in the mPFC were only observed in male MAM-treated animals, and adolescent JQ1 treatment did not affect cellular memory in either sex. The results from the electrophysiological study are consistent with proteomic data showing changes in synaptic proteins only in male MAM-treated rats. On the other hand, protein alterations induced by JQ1 did not affect cortical LTP. Thus, prenatal MAM treatment might change the responsiveness to BET protein functions during adolescence.
JQ1 treatment during adolescence might affect the direction of prefrontal cortical development in both sexes by modulating gene expression and the protein landscape. Although there are some limitations, and caution is required in comparison animal and human studies, the aforementioned results might be important not only for determining the emergence of schizophrenia but also for the administration of JQ1 therapy to this age group, which might be practised in children who are oncology patients [50].