Oxathiapiprolin, a Novel Chemical Inducer Activates the Plant Disease Resistance

Oxathiapiprolin was developed as a specific plant pathogenic oomycete inhibitor, previously shown to have highly curative and protective activities against the pepper Phytophthora blight disease under field and greenhouse tests. Therefore, it was hypothesized that oxathiapiprolin might potentially activate the plant disease resistance against pathogen infections. This study investigated the potential and related mechanism of oxathiapiprolin to activate the plant disease resistance using the bacterium Pseudomonas syringae pv tomato (Pst) and plant Arabidopsis interaction as the targeted system. Our results showed that oxathiapiprolin could activate the plant disease resistance against Pst DC3000, a non-target pathogen of oxathiapiprolin, in Arabidopsis, tobacco, and tomato plants. Our results also showed the enhanced callose deposition and H2O2 accumulation in the oxathiapiprolin-treated Arabidopsis under the induction of flg22 as the pathogen-associated molecular pattern (PAMP) treatment. Furthermore, increased levels of free salicylic acid (SA) and jasmonic acid (JA) were detected in the oxathiapiprolin-treated Arabidopsis plants compared to the mock-treated ones under the challenge of Pst DC3000. Besides, the gene expression results confirmed that at 24 h after the infiltration with Pst DC3000, the oxathiapiprolin-treated Arabidopsis plants had upregulated expression levels of the respiratory burst oxidase homolog D (RBOHD), JA-responsive gene (PDF1.2), and SA-responsive genes (PR1, PR2, and PR5) compared to the control. Taken together, oxathiapiprolin is identified as a novel chemical inducer which activates the plant disease resistance against Pst DC3000 by enhancing the callose deposition, H2O2 accumulation, and hormone SA and JA production.


Introduction
Plants encounter numerous biotic and abiotic factors during their growth and development. Among the biotic factors, plant pathogens are the ones that cause many destructive plant diseases which result in substantial yield and economic losses [1]. However, plants have evolved a complex innate defense system to prevent the infections of diverse pathogens [2,3]. Two levels of immune responses, including the pathogen/microbe-associated molecular patterns (PAMPs/MAMPs) triggered immunity blight under field and greenhouse tests [37]. Also, the protective activity of OX was better under the greenhouse conditions [37]. Because OX has better protective activity, we hypothesized that OX might have the potential to activate the plant disease resistance against the other pathogen infections in addition to oomycetes. To evaluate the potential function and investigate the related underlying mechanisms of OX in inducing plant disease resistance, the related experiments were conducted by mainly using the plant Arabidopsis and bacterium Pst DC3000 (a non-target bacterial pathogen of OX) interaction system. In addition, Nicotiana benthamiana (tobacco) and Solanum lycopersicum (tomato) were also utilized in the related study. For the outcome of the study, we identified that OX was a novel chemical inducer, which could activate the Arabidopsis plant disease resistance against Pst DC3000 through enhancing the callose deposition, H 2 O 2 accumulation, and SA and JA accumulation.

OX Induced the Plant Disease Resistance against Pst DC3000 Infection
To evaluate the potential role of OX in activating the plant disease resistance against the bacterial pathogen Pst DC3000, two types of treatments were designed and conducted. Firstly, the Arabidopsis plants were sprayed with OX (40 µg/mL) or mock (water with DMSO) to the leaves of the whole plants.
Then the Arabidopsis leaves were challenged with the Pst DC3000 by the infiltration using a 1 mL syringe at two days after the pre-treatment of OX. The bacterial growth was examined at three days post infiltration (dpi). Compared to the mock plants, the Pst DC3000 growth was reduced significantly in the OX-treated plant leaves ( Figure 1A). Furthermore, the lower three leaves of another set of Arabidopsis plants were firstly injected with OX (40 µg/mL) or mock (water with DMSO). Two days after the OX injection, the upper three or four Arabidopsis leaves were infiltrated with the Pst DC3000 with a 1 mL syringe. The bacterial growth was examined at 3 dpi. Compared to the mock plants, the Pst DC3000 growth was reduced significantly in the OX-treated plants ( Figure 1B).
other pathogen infections in addition to oomycetes. To evaluate the potential function and investigate the related underlying mechanisms of OX in inducing plant disease resistance, the related experiments were conducted by mainly using the plant Arabidopsis and bacterium Pst DC3000 (a nontarget bacterial pathogen of OX) interaction system. In addition, Nicotiana benthamiana (tobacco) and Solanum lycopersicum (tomato) were also utilized in the related study. For the outcome of the study, we identified that OX was a novel chemical inducer, which could activate the Arabidopsis plant disease resistance against Pst DC3000 through enhancing the callose deposition, H2O2 accumulation, and SA and JA accumulation.

OX Induced the Plant Disease Resistance against Pst DC3000 Infection
To evaluate the potential role of OX in activating the plant disease resistance against the bacterial pathogen Pst DC3000, two types of treatments were designed and conducted. Firstly, the Arabidopsis plants were sprayed with OX (40 μg/mL) or mock (water with DMSO) to the leaves of the whole plants. Then the Arabidopsis leaves were challenged with the Pst DC3000 by the infiltration using a 1 mL syringe at two days after the pre-treatment of OX. The bacterial growth was examined at three days post infiltration (dpi). Compared to the mock plants, the Pst DC3000 growth was reduced significantly in the OX-treated plant leaves ( Figure 1A). Furthermore, the lower three leaves of another set of Arabidopsis plants were firstly injected with OX (40 μg/mL) or mock (water with DMSO). Two days after the OX injection, the upper three or four Arabidopsis leaves were infiltrated with the Pst DC3000 with a 1 mL syringe. The bacterial growth was examined at 3 dpi. Compared to the mock plants, the Pst DC3000 growth was reduced significantly in the OX-treated plants ( Figure  1B).
Besides, OX-induced plant disease resistance against the bacterial pathogen Pst DC3000 was evaluated in tobacco and tomato plants. The tobacco and tomato plants were sprayed with OX (40 μg/mL) or mock (water with DMSO) on the leaves of the whole plants. Two days after the pretreatment, the tomato or tobacco leaves were challenged with the Pst DC3000 by the infiltration with a 1 mL syringe. The bacterial growth was examined at 3 dpi. Compared to the mock plants, the Pst DC3000 growth was reduced significantly in the OX-treated tobacco or tomato leaves ( Figure S1 A and B), which was consistent with the results in Arabidopsis. The Arabidopsis plants (Col-0) were firstly sprayed with OX (40 μg/mL) or mock (water with DMSO). Then the Arabidopsis leaves were infiltrated with the Pst DC3000 (1 × 10 5 Colony Forming Unit (CFU)/mL, OD600 = 0.0002) using a 1 mL syringe two days after the spray treatment. Three days after pathogen inoculation, the bacterial growth was quantified using the plate assay. (B) OX activated the plant disease resistance against the Pst DC3000 infection via pre-infiltration in local leaves. The lower leaves were pre-infiltrated with OX (40 μg/mL) or mock (water with DMSO) two days before the pathogen Pst DC3000 inoculation. The upper leaves were infiltrated with the Pst DC3000 (1 × 10 5 CFU/mL, OD600 = 0.0002) using a 1 mL syringe. Three days after the pathogen inoculation, the bacterial growth was quantified using the plate assay. Significant differences between the OX-treated plants The Arabidopsis plants (Col-0) were firstly sprayed with OX (40 µg/mL) or mock (water with DMSO). Then the Arabidopsis leaves were infiltrated with the Pst DC3000 (1 × 10 5 Colony Forming Unit (CFU)/mL, OD 600 = 0.0002) using a 1 mL syringe two days after the spray treatment. Three days after pathogen inoculation, the bacterial growth was quantified using the plate assay. (B) OX activated the plant disease resistance against the Pst DC3000 infection via pre-infiltration in local leaves. The lower leaves were pre-infiltrated with OX (40 µg/mL) or mock (water with DMSO) two days before the pathogen Pst DC3000 inoculation. The upper leaves were infiltrated with the Pst DC3000 (1 × 10 5 CFU/mL, OD 600 = 0.0002) using a 1 mL syringe. Three days after the pathogen inoculation, the bacterial growth was quantified using the plate assay. Significant differences between the OX-treated plants and the mock were indicated by the asterisks determined from the Student's t-test (p < 0.05). Results shown (means ± SD) are from one of the three independent repeats with the consistent results.
Besides, OX-induced plant disease resistance against the bacterial pathogen Pst DC3000 was evaluated in tobacco and tomato plants. The tobacco and tomato plants were sprayed with OX (40 µg/mL) or mock (water with DMSO) on the leaves of the whole plants. Two days after the pre-treatment, the tomato or tobacco leaves were challenged with the Pst DC3000 by the infiltration with a 1 mL syringe. The bacterial growth was examined at 3 dpi. Compared to the mock plants, the Pst DC3000 growth was reduced significantly in the OX-treated tobacco or tomato leaves ( Figure S1A,B), which was consistent with the results in Arabidopsis.
To further confirm that the reduced growth of bacteria was due to the OX-induced plant disease resistance, the direct inhibition effect of OX against the Pst DC3000 was tested by the in-vitro plate assay. The results showed that OX had no direct inhibition effect against the Pst DC3000 (Figure 2A,B). All these results indicated that OX could induce the plant disease resistance against the Pst DC3000 in Arabidopsis, tomato, and tobacco plants. and the mock were indicated by the asterisks determined from the Student's t-test (p < 0.05). Results shown (means ± SD) are from one of the three independent repeats with the consistent results.
To further confirm that the reduced growth of bacteria was due to the OX-induced plant disease resistance, the direct inhibition effect of OX against the Pst DC3000 was tested by the in-vitro plate assay. The results showed that OX had no direct inhibition effect against the Pst DC3000 ( Figure  2A,B). All these results indicated that OX could induce the plant disease resistance against the Pst DC3000 in Arabidopsis, tomato, and tobacco plants. OX had no direct inhibition effect against the Pst DC3000 via the in-vitro plate assay. The direct inhibition effect of OX against the Pst DC3000 was performed by using King's B agar amended with mock (DMSO) or OX (40 μg/mL). About 100 μL bacterial suspension (5 × 10 3 CFU/mL) was uniformly plated on the surface of the agar plate. The photos of the plates (A) were taken, and the colony number (B) was counted after 2 days of darkness-incubation at 28 °C. Three replicates were prepared for each treatment. Results shown (means ± SD) are from one of the three independent repeats with the consistent results.

OX Activated the Plant Disease Resistance by Enhancing the H2O2 Accumulation in Arabidopsis Plants
To investigate the underlying mechanism of OX-induced plant disease resistance, the H2O2 accumulation was compared between the mock and OX-treated Arabidopsis plants. For both real-time and total quantification of H2O2, there were no significant differences in the chemiluminescence accumulation between the mock-and OX-treated Arabidopsis without the flg22 treatment (a peptide corresponding to the most conserved domain of the bacterial flagellin, acting as a common and potent elicitor of PTI). However, the chemiluminescence accumulation was significantly increased in the OX-treated Arabidopsis plants with the flg22 treatment compared to the mock-treated ones ( Figure  3A,B). The result indicated the enhanced ROS burst in the OX-treated Arabidopsis plants. Further experiments were conducted to quantify the gene expression level of the ROS related gene, such as the respiratory burst oxidase homolog D (RBOHD), which plays a crucial role in the accumulation of ROS in apoplast [38]. The qPCR result showed that the transcription level of RBOHD was not significantly changed between the mock-and OX-treated Arabidopsis plants before the Pst DC3000 treatment. However, the OX-treated Arabidopsis plants had significantly higher expression level of RBOHD than that of the mock-treated ones at 24-h post infiltration (hpi) with the Pst DC3000 ( Figure  3C), which was consistent with the increased H2O2 accumulation in response to the flg22 treatment. OX had no direct inhibition effect against the Pst DC3000 via the in-vitro plate assay. The direct inhibition effect of OX against the Pst DC3000 was performed by using King's B agar amended with mock (DMSO) or OX (40 µg/mL). About 100 µL bacterial suspension (5 × 10 3 CFU/mL) was uniformly plated on the surface of the agar plate. The photos of the plates (A) were taken, and the colony number (B) was counted after 2 days of darkness-incubation at 28 • C. Three replicates were prepared for each treatment. Results shown (means ± SD) are from one of the three independent repeats with the consistent results.

OX Activated the Plant Disease Resistance by Enhancing the H 2 O 2 Accumulation in Arabidopsis Plants
To investigate the underlying mechanism of OX-induced plant disease resistance, the H 2 O 2 accumulation was compared between the mock and OX-treated Arabidopsis plants. For both real-time and total quantification of H 2 O 2 , there were no significant differences in the chemiluminescence accumulation between the mock-and OX-treated Arabidopsis without the flg22 treatment (a peptide corresponding to the most conserved domain of the bacterial flagellin, acting as a common and potent elicitor of PTI). However, the chemiluminescence accumulation was significantly increased in the OX-treated Arabidopsis plants with the flg22 treatment compared to the mock-treated ones ( Figure 3A,B). The result indicated the enhanced ROS burst in the OX-treated Arabidopsis plants. Further experiments were conducted to quantify the gene expression level of the ROS related gene, such as the respiratory burst oxidase homolog D (RBOHD), which plays a crucial role in the accumulation of ROS in apoplast [38]. The qPCR result showed that the transcription level of RBOHD was not significantly changed between the mock-and OX-treated Arabidopsis plants before the Pst DC3000 treatment. However, the OX-treated Arabidopsis plants had significantly higher expression level of RBOHD than that of the mock-treated ones at 24-h post infiltration (hpi) with the Pst DC3000 ( Figure 3C), which was consistent with the increased H 2 O 2 accumulation in response to the flg22 treatment. All these results indicated that OX induced the plant disease resistance potentially by enhancing the H 2 O 2 accumulation in Arabidopsis plants upon the pathogen Pst DC3000 inoculation.
All these results indicated that OX induced the plant disease resistance potentially by enhancing the H2O2 accumulation in Arabidopsis plants upon the pathogen Pst DC3000 inoculation. The relative expression levels of RBOHD in mock-and OX-treated Arabidopsis leaves before and after the Pst DC3000 inoculation. ACTIN was used as the internal reference gene. Significant differences between the OX-treated plants and the mock were indicated by the asterisks determined from the one-way analysis of variance (ANOVA) statistical test using SPSS ver. 21 (p < 0.05). Results shown (means ± SD) are from one of the two independent repeats with the consistent results.

OX Activated the Plant Disease Resistance by Increasing the Callose Deposition in Arabidopsis Plants
To further investigate the underlying mechanism of OX-induced plant disease resistance, the callose deposition, which is an indicator of cell wall defense [39,40], was compared between the mockand OX-treated Arabidopsis plants ( Figure 4A,B). The callose deposition levels were low and similar between the mock-and OX-treated Arabidopsis plants with the water treatment. While the callose deposition was significantly increased in both the mock-and OX-treated Arabidopsis plants with the flg22 treatment. However, the callose deposition was significantly higher in the OX-treated Arabidopsis plants than the mock-treated ones after the flg22 treatment, indicating an enhanced cell wall-related defense response in the OX-treated Arabidopsis plants during the interactions with the bacterial pathogen. The relative expression levels of RBOHD in mock-and OX-treated Arabidopsis leaves before and after the Pst DC3000 inoculation. ACTIN was used as the internal reference gene. Significant differences between the OX-treated plants and the mock were indicated by the asterisks determined from the one-way analysis of variance (ANOVA) statistical test using SPSS ver. 21 (p < 0.05). Results shown (means ± SD) are from one of the two independent repeats with the consistent results.

OX Activated the Plant Disease Resistance by Increasing the Callose Deposition in Arabidopsis Plants
To further investigate the underlying mechanism of OX-induced plant disease resistance, the callose deposition, which is an indicator of cell wall defense [39,40], was compared between the mock-and OX-treated Arabidopsis plants ( Figure 4A,B). The callose deposition levels were low and similar between the mock-and OX-treated Arabidopsis plants with the water treatment. While the callose deposition was significantly increased in both the mock-and OX-treated Arabidopsis plants with the flg22 treatment. However, the callose deposition was significantly higher in the OX-treated Arabidopsis plants than the mock-treated ones after the flg22 treatment, indicating an enhanced cell wall-related defense response in the OX-treated Arabidopsis plants during the interactions with the bacterial pathogen. . Then the Arabidopsis leaves were treated with water or 100 μM flg22 using the infiltration with a 1 mL syringe two days after the spray treatment. The leaves were then stained using 0.01% aniline blue for the callose deposition after 14-16 hpi of flg22 treatment. (A) The images of callose deposition in Arabidopsis leaves were taken by a Nikon Eclipse 80i epi-fluorescent microscope (Nikon, Tokyo, Japan). (B) The number of callose deposition points was counted by the Image J software. Significant differences between the OXtreated plants and the mock-treated plants were indicated by the asterisks determined from the Student's t-test (p < 0.05). Results shown (means ± SD) are from one of the two independent repeats with consistent results.

OX Activated the Plant Disease Resistance by Enhancing both the SA and JA Related Pathways in Arabidopsis Plants
To further investigate the underlying mechanism of OX-induced plant disease resistance, the levels of several plant hormones in the mock-and OX-treated Arabidopsis plants before and after the Pst DC3000 inoculation were quantified ( Figure 5). The levels of free SA and JA did not show significant differences in the mock-and OX-treated Arabidopsis plants without the challenge of Pst DC3000. However, compared to the mock-treated ones, both free SA and free JA levels were significantly higher in the OX-treated Arabidopsis plants under the challenge of the Pst DC3000 ( Figure  5A,C). The levels of SA-Gly (glycosylated derivatives of salicylic acid), JA-Ile/Leu, and ABA had no significant differences in the mock-and OX-treated Arabidopsis plants with or without the Pst DC3000 inoculation ( Figure 5B,D,E). The hormone results indicated that OX activated the plant disease resistance by enhancing the accumulation of both SA and JA.
Further, the transcription levels of several typical genes in SA and JA related pathways were quantified. First, the transcription levels of SA-responsive genes were analyzed, including PR1, PR2, and PR5. Although SA accumulation did not differ significantly without the Pst DC3000 inoculation, were firstly sprayed with OX (40 µg/mL) or mock (water with DMSO). Then the Arabidopsis leaves were treated with water or 100 µM flg22 using the infiltration with a 1 mL syringe two days after the spray treatment. The leaves were then stained using 0.01% aniline blue for the callose deposition after 14-16 hpi of flg22 treatment. (A) The images of callose deposition in Arabidopsis leaves were taken by a Nikon Eclipse 80i epi-fluorescent microscope (Nikon, Tokyo, Japan). (B) The number of callose deposition points was counted by the Image J software. Significant differences between the OX-treated plants and the mock-treated plants were indicated by the asterisks determined from the Student's t-test (p < 0.05). Results shown (means ± SD) are from one of the two independent repeats with consistent results.

OX Activated the Plant Disease Resistance by Enhancing both the SA and JA Related Pathways in Arabidopsis Plants
To further investigate the underlying mechanism of OX-induced plant disease resistance, the levels of several plant hormones in the mock-and OX-treated Arabidopsis plants before and after the Pst DC3000 inoculation were quantified ( Figure 5). The levels of free SA and JA did not show significant differences in the mock-and OX-treated Arabidopsis plants without the challenge of Pst DC3000. However, compared to the mock-treated ones, both free SA and free JA levels were significantly higher in the OX-treated Arabidopsis plants under the challenge of the Pst DC3000 ( Figure 5A,C). The levels of SA-Gly (glycosylated derivatives of salicylic acid), JA-Ile/Leu, and ABA had no significant differences in the mock-and OX-treated Arabidopsis plants with or without the Pst DC3000 inoculation ( Figure 5B,D,E). The hormone results indicated that OX activated the plant disease resistance by enhancing the accumulation of both SA and JA. treatment ( Figure 6D), which was consistent with the JA accumulation. Consistent with the similar accumulation of ABA with or without the challenge of Pst DC3000, the transcription levels of two ABA related genes, ABI4 and ABA2, showed the similar levels in both the OX-treated and mocktreated Arabidopsis plants at 24 hpi with the Pst DC3000 inoculation ( Figure 6E,F). Taken together, both SA-and JA-signaling pathways might contribute to the OX-induced plant disease resistance in Arabidopsis, while ABA might not be very important for the OX-induced plant disease resistance. were sprayed with OX (40 μg/mL) or mock (water with DMSO). The Arabidopsis leaves were infiltrated with the Pst DC3000 (5 × 10 5 CFU/mL, OD600 = 0.001) with a 1 mL syringe two days after the spray treatment. The samples were collected for hormone quantification at 2 dpi. Significant differences between the OX-treated plants and the mock were indicated using the asterisks determined from the Student's t-test (p < 0.05). . The Arabidopsis leaves were infiltrated with the Pst DC3000 (5 × 10 5 CFU/mL, OD 600 = 0.001) with a 1 mL syringe two days after the spray treatment. The samples were collected for hormone quantification at 2 dpi. Significant differences between the OX-treated plants and the mock were indicated using the asterisks determined from the Student's t-test (p < 0.05).
Further, the transcription levels of several typical genes in SA and JA related pathways were quantified. First, the transcription levels of SA-responsive genes were analyzed, including PR1, PR2, and PR5. Although SA accumulation did not differ significantly without the Pst DC3000 inoculation, the significantly higher transcription levels of PR genes were shown in the OX-treated Arabidopsis plants compared to the mock-treated ones, except PR2 ( Figure 6A-C). Consistent with the hormone results, the OX-treated Arabidopsis plants had significantly higher expression levels of these three PR genes than the mock-treated ones at 24 hpi with the Pst DC3000 treatment ( Figure 6A-C). Next, a typical JA-responsive gene PDF1.2 was analyzed, which is important for the defense against the necrotrophic pathogens [41]. The result showed that the expression level of PDF 1.2 was significantly higher in the OX-treated Arabidopsis plants than the mock-treated ones at 24 hpi with the Pst DC3000 treatment ( Figure 6D), which was consistent with the JA accumulation. Consistent with the similar accumulation of ABA with or without the challenge of Pst DC3000, the transcription levels of two ABA related genes, ABI4 and ABA2, showed the similar levels in both the OX-treated and mock-treated Arabidopsis plants at 24 hpi with the Pst DC3000 inoculation ( Figure 6E,F). Taken together, both SAand JA-signaling pathways might contribute to the OX-induced plant disease resistance in Arabidopsis, while ABA might not be very important for the OX-induced plant disease resistance. ACTIN was used as the internal reference gene. Significant differences between the OX-treated plants and the mock-treated plants were indicated by the one-way ANOVA statistical test by SPSS ver. 21 (p < 0.05). Results shown (means ± SD) are from one of the two independent repeats with the consistent results.

Discussion
OX was firstly synthesized as a fungicide to control oomycete-associated plant diseases by the DuPont Company in 2007 [32,33]. Previous research indicated that OX exhibited direct and high inhibition activity against the growth of many oomycete pathogens with a low dose. The target protein of OX was the oxysterol binding protein (OSBP) in oomycetes [33][34][35][36][37]. Besides, OX exhibited both curative and protective activity against pepper Phytophthora blight, and the protective activity was better [37]. There is no further study on the potential of OX to induce the plant immunity against the other pathogens in addition to oomycete pathogens. Therefore, in the present study, the related responses and mechanisms of the induced plant disease resistance by OX was investigated mainly using the Pst DC3000 and Arabidopsis interaction system.
The Pst DC3000 and Arabidopsis interaction system is widely used in studying the interactions of pathogens and hosts [42]. Here, we found that spraying the Arabidopsis plants with OX at the concentration of 40 μg/mL two days before the pathogen Pst DC3000 inoculation could enhance the Arabidopsis plant defense against the Pst DC3000. A further experiment showed that the infiltrated local leaves of Arabidopsis with OX could enhance the defense of the systemic leaves against Pst DC3000. Besides, spraying treatment of the plants with OX at 40 μg/mL could activate the plant disease resistance against the Pst DC3000 in tobacco and tomato plants. All these results indicated that OX could serve as a common inducer for plant immunity to activate the plant disease resistance against the Pst DC3000 infection in diverse plant species.

Discussion
OX was firstly synthesized as a fungicide to control oomycete-associated plant diseases by the DuPont Company in 2007 [32,33]. Previous research indicated that OX exhibited direct and high inhibition activity against the growth of many oomycete pathogens with a low dose. The target protein of OX was the oxysterol binding protein (OSBP) in oomycetes [33][34][35][36][37]. Besides, OX exhibited both curative and protective activity against pepper Phytophthora blight, and the protective activity was better [37]. There is no further study on the potential of OX to induce the plant immunity against the other pathogens in addition to oomycete pathogens. Therefore, in the present study, the related responses and mechanisms of the induced plant disease resistance by OX was investigated mainly using the Pst DC3000 and Arabidopsis interaction system.
The Pst DC3000 and Arabidopsis interaction system is widely used in studying the interactions of pathogens and hosts [42]. Here, we found that spraying the Arabidopsis plants with OX at the concentration of 40 µg/mL two days before the pathogen Pst DC3000 inoculation could enhance the Arabidopsis plant defense against the Pst DC3000. A further experiment showed that the infiltrated local leaves of Arabidopsis with OX could enhance the defense of the systemic leaves against Pst DC3000. Besides, spraying treatment of the plants with OX at 40 µg/mL could activate the plant disease resistance against the Pst DC3000 in tobacco and tomato plants. All these results indicated that OX could serve as a common inducer for plant immunity to activate the plant disease resistance against the Pst DC3000 infection in diverse plant species.
Reactive oxygen species (ROS) are widely distributed in plants under different conditions, including the challenges of both abiotic and biotic stresses. ROS are important factors to regulate the cellular process and signaling transduction in plants. ROS also play important roles in plant immune response against the pathogen infections [43]. Under the attack of pathogens, plants will activate defense responses, such as the oxidative burst to produce ROS at the infection site, which is one of the earliest events of a plant's defense responses [44]. Various subcellular compartments of plants can produce ROS, including the mitochondria, chloroplasts, cell wall, plasma membrane, and peroxisomes/glyoxysome [43]. However, the apoplastic ROS, mainly produced at the plasma membrane, are considered as the major ones during the interactions of plants and pathogens [43]. The NADPH oxidases, a member of the respiratory burst oxidase homolog (RBOH) family, are very important in ROS production. They can transfer electrons from cytosolic NADPH or NADH to the apoplastic oxygen and finally involve in the production of hydrogen peroxide (H 2 O 2 ) [43][44][45]. In Arabidopsis, there are 10 members of RBOHs, among which RBOHD is the most studied and plays an important role in plant immunity [38,45]. In this study, the levels of H 2 O 2 were quantified using the Glomax 20/20 single-well luminometer (Promega), and the transcription level of RBOHD was quantified using real-time qPCR. Compared to the mock-treated Arabidopsis plants, the H 2 O 2 accumulation and the transcription level of RBOHD were significantly increased in the OX-treated Arabidopsis plants with the flg22 treatment or the Pst DC3000 inoculation. The results indicated that the ROS burst may contribute to the OX-induced plant disease resistance.
The plant cell wall is one of the first barriers of plants to prevent themselves from the pathogen infections [46]. Plants can enhance the depositions of several polymers, such as employ the toxic compounds, phenolic complexes, and callose to defend themselves against pathogen infections [47]. Among these polymers, callose is a polymer with a high-molecular weight, and it consists of β-(1,3)-glucan, which can be induced by the PAMPs, such as flg22 [39]. The callose deposition is a judging method widely used to evaluate the plant PTI [39,40]. In this study, the callose deposition was visualized using the aniline blue staining based on the previous report [40]. Compared to the mock-treated Arabidopsis plants, the callose deposition was significantly higher in the OX-treated Arabidopsis plants under the induction of flg22, indicating a strengthened cell wall in the OX-treated Arabidopsis plants, which might contribute to the OX-induced plant disease resistance.
Plant hormones are important for the development and defense of plants. There are nine major plant hormones. Among them, salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) are the main hormones to regulate plant immunity under different biotic and abiotic stresses [48,49]. These three main pathways could cross-talk with the other hormone pathways to confer resistance against diverse pathogen infections [48,49]. Generally, SA is associated with the plant defense against the hemi-biotrophic and biotrophic pathogens. In contrast, JA plays a more important role in plant defense against the necrotrophic pathogens [48]. The functions of SA and JA in plant immunity could be antagonistic [49,50]. However, literature has shown that SA and JA could cooperate with each other and function synergistically in plant immunity [51][52][53]. In general, to defend against the infections of the necrotrophic and biotrophic pathogens, ABA is a negative regulator in plants [49]. However, there is evidence showing that ABA could also be a positive regulator of plant defense, such as Arabidopsis plant resistance against the Pst DC3000 (a hemi-biotrophic pathogen) at the early stage, by inducing plant stomata closure [54]. Therefore, the exact function of ABA in plant defense should be determined by different plant-pathogen interactions at different infection stages [49].
Plant hormones are very important in plant defense. Whether the hormone pathways contributed to the OX-induced plant disease resistance remains unclear. In this study, we found that under the challenge of the Pst DC3000, both the free SA and JA levels were significantly increased in the OX-treated Arabidopsis plants than those of the mock-treated Arabidopsis plants at 48 hpi. The levels of SA-Gly, JA-Ile/Leu, and ABA had no significant differences in the mock-and OX-treated Arabidopsis plants. In addition, the real-time qPCR results showed that the OX-treated Arabidopsis plants had higher transcription levels of three PR genes, including PR1, PR2, and PR. The level of the JA-responsive gene, PDF 1.2, was also significantly enhanced compared to the mock-treated Arabidopsis plants. However, the plants showed similar transcription levels of two ABA signaling genes, ABI4 and ABA2. Taken together, we discovered that both SA and JA pathways were activated in the OX-treated Arabidopsis plants upon the Pst DC3000 inoculation, which indicated that both SA-and JA-signaling pathways might contribute to the OX-induced plant disease resistance in Arabidopsis and these pathways might work synergistically. However, the ABA pathway might not be significantly involved in the related plant disease resistance.
The mechanisms of the OX-induced and the BTH or BABA-induced plant disease resistance have similarities and differences. Literature shows that BTH or BABA can induce the plant broad-spectrum disease resistance [19][20][21][22][23][24][25]. For instance, BTH can induce the plant disease resistance against turnip crinkle virus, Peronospora parasitica, and Pst DC3000 in Arabidopsis [19]. BABA can induce the plant disease resistance against B. cinerea and P. parasitica in Arabidopsis [22,23]. For the present study, we found that OX could induce the plant disease resistance against the Pst DC3000 in Arabidopsis, tobacco and tomato plants. Whether OX could induce the plant broad-spectrum disease resistance against other pathogens needs to be further investigated. Besides, the mechanisms of BTH or BABA-induced plant disease resistance are complicated and dependent on the systems of the plant-pathogen interaction. For example, BTH can induce the plant disease resistance against B. cinerea by modifying the amino acid profile in grape [55]. BABA can induce the plant disease resistance against Plasmopara viticola by enhancing the callose formation and JA signaling or by the accumulation of lignin in grape plants [24,56]. Both BTH and BABA can induce the plant disease resistance against the Pst DC3000, mainly by activating the SA signaling pathway, such as the increased accumulation of SA and PR proteins [19,23]. However, in the present study, we found that OX activated the plant disease resistance against the Pst DC3000 by increasing the accumulation of both SA and JA and their responsive genes. Whether the OX-induced plant disease resistance is dependent on both SA and JA signaling pathways needs to be further studied.
In summary, in this study, we discovered that OX was a novel chemical inducer to activate the plant disease resistance against the Pst DC3000 in Arabidopsis by enhancing the callose deposition, H 2 O 2 accumulation, and SA and JA production and related signaling pathways. Future research can be carried out to investigate the potential and associated mechanisms of OX as an important plant disease resistance inducer against other plant pathogens, such as bacteria, fungi, oomycetes, and viruses in different plant species. Such research will facilitate our understanding of the mechanism of the induced plant disease resistance and its application in agriculture to enhance plant health against diverse pathogen infections.

Plant Growth Conditions
Wild-type Arabidopsis thaliana (Col-0) plants and tobacco (Nicotiana benthamiana) plants were cultured in a growth room at 22 • C with 10 h light/14 h darkness for Arabidopsis thaliana (Col-0) and 16 h light/8 h darkness for tobacco, respectively. Tomato (Solanum lycopersicum) plants (cv. OH88119) were cultivated in a greenhouse at room temperature with 12 h light/12 h darkness. The pathogen inoculation experiments were conducted 4-5 weeks after the transplanting.

The OX Treatment, Pst DC3000 Infiltration, and Bacteria Growth Assay
OX, from DuPont Crop Protection (Wilmington, DE, USA), was dissolved in DMSO for a stocking solution at 4 × 10 4 µg/mL. The stocking solution was 1000 times diluted in water for a final concentration of 40 µg/mL. To evaluate the potential role of OX in activating the plant disease resistance against the pathogen Pst DC3000 infection, the Arabidopsis, tobacco, and tomato plants were sprayed with OX solution (40 µg/mL) or mock (water containing the same amount of DMSO) on leaves firstly. Two days after the spray treatment, the leaves were infiltrated with 10 mM MgCl 2 containing the Pst DC3000 using a 1 mL syringe. The bacteria concentration was 1 × 10 5 CFU/mL (OD 600 = 0.0002). Another test for the OX-induced plant disease resistance in Arabidopsis was through the treatment of the local leaves. For example, the lower leaves of Arabidopsis plants were infiltrated with the OX solution (40 µg/mL) or mock firstly. Two days later, the upper three or four leaves were infiltrated with bacterial broth, as described above. Three days after the infiltration with the Pst DC3000, three leaf discs were collected from the bacteria infiltrated leaves as one sample for the bacteria growth assay [57]. Each treatment had at least three replicates, and the whole experiment was conducted three times with consistent results.

The Direct Inhibition Effect of OX against the Pst DC3000 by the in-Vitro Plate Assay
The direct inhibition effect of OX against the Pst DC3000 was performed by the in-vitro plate inhibition assay. King's B agar amended with 40 µg/mL OX and the concentration of DMSO was limited to 0.1% (v/v). An equivalent concentration of DMSO was used for the negative controls. Bacterial suspension (100 µL, 5 × 10 3 CFU/mL) was uniformly plated on the agar surface. The colony number was counted after 2 days of darkness-incubation at 28 • C. Three replicates were prepared for each treatment, and the entire experiment was performed three times with consistent results.

The H 2 O 2 Detection and Callose Deposition
The H 2 O 2 was detected according to a previous report with a minor modification [58]. The mock or OX-treated Arabidopsis leaves were sliced into 5 mm diameter discs and incubated in the plastic plates containing the distilled water for 12 h. Three leaf discs were treated with water or 200 nM flg22 (GeneScript, Piscataway, NJ, USA) as the PAMP treatment in 200 µL buffer containing 20 µM luminol and 1 µg/mL horseradish peroxidase. Luminescence was continuously measured for 30 min by using the Glomax 20/20 single well luminometer (Promega, Madison ,WI, USA). Each treatment had at least three replicates and the whole experiment was conducted twice with the consistent results.
The callose deposition was measured based on a protocol reported by Lin Jin [40]. In brief, the mock or OX-treated Arabidopsis plants were infiltrated with 100 µM flg22 or water. The infiltrated leaves were stained for callose by 0.01% aniline blue (Cat #: 415049, Sigma-Aldrich, St. Louis, MO, USA) after 14-16 h after infiltration (hpi). The callose deposition was visualized by a Nikon Eclipse 80i epi-fluorescent microscope (Nikon, Tokyo, Japan) and further quantified by Image J software (http://rsbweb.nih.gov/ij/). Each treatment included six plants, and the whole experiment was conducted twice with consistent results.

Plant Hormone Extraction and Quantification
Plant hormones were extracted and detected according to the previous reports [59,60]. In brief, the mock or OX-treated Arabidopsis plants were infiltrated with buffer (10 mM MgCl 2 ) or buffer containing the Pst DC3000 cells. The bacterial cell concentration was 5 × 10 5 Colony Forming Unit (CFU)/mL (OD 600 = 0.001). Two days after infiltration, the leaf samples (approximately 110 mg fresh weight per sample) were collected and extracted with 400 µL extraction buffer containing 10% methanol and 1% acetic acid. Isotope labelled internal standards (d 4 -SA, d 5 -JA, and d 6 -ABA) were added at the beginning of the extraction. The amounts of the internal standards added were 1 ng of 2 H6-ABA (d 6 (59), and JA-Ile/Leu 322 (130). The daughter masses were denoted in the brackets listed above. Each treatment had five replicates.

RNA Extraction and Gene Expression Quantification
The total RNA of the Arabidopsis leaves was extracted using TRIzol Reagent (Invitrogen, Carlsbad, MA, USA) as the described protocol. First-strand cDNA was synthesized using the Applied Biosystems reverse transcription kit (Applied Biosystem, Waltham, MA, USA, catalog number: 4368814). Gene expression was quantified using the real-time qPCR with the CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA, USA). Data was analyzed using the 2 −∆Ct method. ACTIN was used as the internal reference gene. The primers used in this study were listed in Table S1.
Supplementary Materials: Supplementary materials can be found at http://www.mdpi.com/1422-0067/21/4/1223/ s1. Figure S1: OX activated the plant disease resistance against the Pst DC3000 infection in tobacco (A) and tomato (B) plants. The tobacco or tomato plants were sprayed with OX (40 µg/mL) or mock (water with DMSO). The leaves were infiltrated with the Pst DC3000 (1 × 10 5 CFU/mL, OD 600 = 0.0002) using a 1 mL syringe two days after the spray treatment. Three days after the pathogen inoculation, the bacterial growth was quantified by the plate assay. Significant differences between the OX-treated plants and the mock were indicated by the asterisks determined from the Student's t-test (p < 0.05). Results shown (means ± SD) are from one of the three independent repeats with the consistent results. Table S1: Primers used for qPCR in this study.