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Article

Functional Analysis of Long Non-Coding RNAs Reveal Their Novel Roles in Biocontrol of Bacteria-Induced Tomato Resistance to Meloidogyne incognita

1
College of Plant Protection, Shenyang Agricultural University, Dongling Road 120, Shenyang 110866, China
2
College of Plant Protection, Jilin Agricultural University, Xincheng Road 2888, Jilin 130118, China
3
College of Biotechnology, Shenyang Agricultural University, Dongling Road 120, Shenyang 110866, China
4
College of Science, Shenyang Agricultural University, Dongling Road 120, Shenyang 110866, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(3), 911; https://doi.org/10.3390/ijms21030911
Received: 23 December 2019 / Revised: 27 January 2020 / Accepted: 28 January 2020 / Published: 30 January 2020
(This article belongs to the Section Molecular Plant Sciences)
Root-knot nematodes (RKNs) severely affect plants growth and productivity, and several commercial biocontrol bacteria can improve plants resistance to RKNs. Pseudomonas putida Sneb821 isolate was found to induce tomatoes resistance against Meloidogyne incognita. However, the molecular functions behind induced resistance remains unclear. Long non-coding RNA (lncRNA) is considered to be a new component that regulates the molecular functions of plant immunity. We found lncRNA was involved in Sneb821-induced tomato resistance to M. incognita. Compared with tomato inoculated with M. incognita, high-throughput sequencing showed that 43 lncRNAs were upregulated, while 35 lncRNAs were downregulated in tomatoes previously inoculated with Sneb821. A regulation network of lncRNAs was constructed, and the results indicated that 12 lncRNAs were found to act as sponges of their corresponding miRNAs. By using qRT-PCR and the overexpression vector pBI121, we found the expression of lncRNA44664 correlated with miR396/GRFs (growth-regulating factors) and lncRNA48734 was correlated with miR156/SPL (squamosal promoter-binding protein-like) transcription factors. These observations provided a novel molecular model in biocontrol bacteria-induced tomato resistance to M. incognita. View Full-Text
Keywords: long non-coding RNA; biocontrol bacteria; Meloidogyne incognita; tomato; induced resistance long non-coding RNA; biocontrol bacteria; Meloidogyne incognita; tomato; induced resistance
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MDPI and ACS Style

Yang, F.; Zhao, D.; Fan, H.; Zhu, X.; Wang, Y.; Liu, X.; Duan, Y.; Xuan, Y.; Chen, L. Functional Analysis of Long Non-Coding RNAs Reveal Their Novel Roles in Biocontrol of Bacteria-Induced Tomato Resistance to Meloidogyne incognita. Int. J. Mol. Sci. 2020, 21, 911. https://doi.org/10.3390/ijms21030911

AMA Style

Yang F, Zhao D, Fan H, Zhu X, Wang Y, Liu X, Duan Y, Xuan Y, Chen L. Functional Analysis of Long Non-Coding RNAs Reveal Their Novel Roles in Biocontrol of Bacteria-Induced Tomato Resistance to Meloidogyne incognita. International Journal of Molecular Sciences. 2020; 21(3):911. https://doi.org/10.3390/ijms21030911

Chicago/Turabian Style

Yang, Fan, Dan Zhao, Haiyan Fan, Xiaofeng Zhu, Yuanyuan Wang, Xiaoyu Liu, Yuxi Duan, Yuanhu Xuan, and Lijie Chen. 2020. "Functional Analysis of Long Non-Coding RNAs Reveal Their Novel Roles in Biocontrol of Bacteria-Induced Tomato Resistance to Meloidogyne incognita" International Journal of Molecular Sciences 21, no. 3: 911. https://doi.org/10.3390/ijms21030911

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