Exopolysaccharide Isolated from Lactobacillus plantarum L-14 Has Anti-Inflammatory Effects via the Toll-Like Receptor 4 Pathway in LPS-Induced RAW 264.7 Cells

Inflammation is a biological response of the immune system to defend the body from negative stimulation. However, the excessive inflammatory response can damage host tissues and pose serious threats. Exopolysaccharide (EPS), one of the postbiotics, is secreted from lactic acid bacteria. Although many studies have described the beneficial effects of EPS, such as its anti-inflammatory and anti-oxidant effects, its underlying mechanisms have remained to be poorly understood. Thus, we identified that EPS obtained from Lactobacillus plantarum L-14 was a homogeneous polysaccharide primarily comprised of glucose. To examine these anti-inflammatory effects, an inflammatory response was induced by lipopolysaccharide (LPS) administration to mouse macrophage RAW 264.7 cells that were pretreated with EPS. The anti-inflammatory effects of EPS were identified by analyzing the changes within inflammatory markers at the molecular level. We demonstrate here that EPS suppressed proinflammatory mediators, such as cyclooxygenase-2, interleukin-6, tumor necrosis factor-α, and interleukin-1β, and downregulated the expression of an inducible nitric oxide synthase known to lead to oxidative stress. It was also confirmed that EPS had anti-inflammatory effects by blocking the interaction of LPS with Toll-like receptor 4 (TLR4), as demonstrated by using the known TLR4 inhibitor TAK-242. In addition, we found that EPS itself could suppress the expression of TLR4. Consequently, our data suggest that EPS can be a potential target for the development of natural product-derived medicine for treating inflammatory diseases related to TLR4.


Introduction
Inflammation has been identified as an essential defense reaction to protect the body from various harmful stimuli, such as infection, toxic compounds, or injury [1]. However, upon aggravation of the inflammatory response, the host may undergo tissue damage and potentially contract a chronic inflammatory disorder such as rheumatoid arthritis, psoriasis, lupus erythematosus, and asthma [2]. Inflammatory bowel disease affects more than 1.4 million people in the USA, with an average annual direct medical cost of 6.3 billion USD [3]. Clearly, systemic regulation of inflammation plays a significant role for disease progression within individuals and strongly impacts public health [4]. Inflammatory the known TLR4 inhibitor TAK-242. The activation of TLR4 and myeloid differentiation factor (MyD88) by LPS is then suppressed by EPS. Furthermore, we could identify that EPS itself suppressed TLR4 expression without LPS stimulation.

Co-Culture with L-14 Inhibited the Induction of Inflammation by LPS in RAW 264.7 Cells
To determine whether co-culture with L-14 suppressed the inflammatory response via interaction with immune cells, the cytokines induced by LPS in mouse macrophage RAW 264.7 cells were quantified via enzyme-linked immunosorbent assay (ELISA). The cells seeded in a 12-well plate were co-cultured with L-14 for 6 h, and the proinflammatory markers IL-6, TNF-α, and monocyte chemoattractant protein-1 (MCP-1) were induced by LPS. As a result, the expression levels of IL-6, TNF-α, and MCP-1 were increased by LPS, but not by L-14 ( Figure 1A). Surprisingly, the release of inflammatory cytokines was significantly decreased to control the levels in cells that had been previously co-cultured with L-14. To confirm whether these results were caused by L-14 affecting cell proliferation, the viability of the RAW 264.7 cells cultured under the same culture conditions was quantified by WST-8 cell viability assay, which showed that cell viability was unaffected by L-14 or LPS ( Figure 1B). These results suggested that the metabolite secreted from L-14 exhibited anti-inflammatory effects by directly interacting with immune cells.
Int. J. Mol. Sci. 2020, 21, x FOR PEER REVIEW 3 of 19 related to the inflammatory pathway were downregulated in the EPS pretreatment group. To examine whether EPS inhibits the interaction between LPS and TLR4, the TLR4 pathway was analyzed using the known TLR4 inhibitor TAK-242. The activation of TLR4 and myeloid differentiation factor (MyD88) by LPS is then suppressed by EPS. Furthermore, we could identify that EPS itself suppressed TLR4 expression without LPS stimulation.

Co-Culture with L-14 Inhibited the Induction of Inflammation by LPS in RAW 264.7 Cells
To determine whether co-culture with L-14 suppressed the inflammatory response via interaction with immune cells, the cytokines induced by LPS in mouse macrophage RAW 264.7 cells were quantified via enzyme-linked immunosorbent assay (ELISA). The cells seeded in a 12-well plate were co-cultured with L-14 for 6 h, and the proinflammatory markers IL-6, TNF-α, and monocyte chemoattractant protein-1 (MCP-1) were induced by LPS. As a result, the expression levels of IL-6, TNF-α, and MCP-1 were increased by LPS, but not by L-14 ( Figure 1A). Surprisingly, the release of inflammatory cytokines was significantly decreased to control the levels in cells that had been previously co-cultured with L-14. To confirm whether these results were caused by L-14 affecting cell proliferation, the viability of the RAW 264.7 cells cultured under the same culture conditions was quantified by WST-8 cell viability assay, which showed that cell viability was unaffected by L-14 or LPS ( Figure 1B). These results suggested that the metabolite secreted from L-14 exhibited antiinflammatory effects by directly interacting with immune cells. To determine whether L-14 could suppress the inflammatory response, RAW 264.7 cells were co-cultured with L-14 for 6 h and then treated 1 μg/mL LPS for 6 h. (A) The production of cytokines stimulated by LPS was quantified via ELISA. Co-culture with L-14 significantly inhibited the release of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and monocyte chemoattractant protein-1 into the media. (B) Viability was not affected by L-14 in RAW 264.7 cells, which suggests that L-14 exhibited anti-inflammatory effects by directly interacting with immune cells. *** p < 0.001 versus the LPS-treated group; ns, not significant.

EPS Isolated from L-14 Was a Homogeneous Polysaccharide Primarily Composed of Glucose
EPS was purified from culture media via ethanol precipitation. Isolated EPS was analyzed by fast protein liquid chromatography (FPLC) size exclusion chromatography, resulting in a single symmetrical peak, indicating that EPS was a homogeneous polysaccharide ( Figure 2A). The monosaccharide components of EPS were determined by Thin layer chromatography (TLC) ( Figure  2B). EPS hydrolysate was expressed at the same point with the glucose used as the standard, so the results indicated that EPS was mainly composed of glucose. We conducted Benedict's test to confirm that EPS is primarily composed of glucose. EPS hydrolysate changed the color of the reagent into orange-red ( Figure 2C). Additionally, the color of the reagent was changed into green by EPS. The characteristic structure of EPS was analyzed using Fourier-transform infrared spectroscopy (FTIR). To determine whether L-14 could suppress the inflammatory response, RAW 264.7 cells were co-cultured with L-14 for 6 h and then treated 1 µg/mL LPS for 6 h. (A) The production of cytokines stimulated by LPS was quantified via ELISA. Co-culture with L-14 significantly inhibited the release of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and monocyte chemoattractant protein-1 into the media. (B) Viability was not affected by L-14 in RAW 264.7 cells, which suggests that L-14 exhibited anti-inflammatory effects by directly interacting with immune cells. *** p < 0.001 versus the LPS-treated group; ns, not significant.

EPS Isolated from L-14 Was a Homogeneous Polysaccharide Primarily Composed of Glucose
EPS was purified from culture media via ethanol precipitation. Isolated EPS was analyzed by fast protein liquid chromatography (FPLC) size exclusion chromatography, resulting in a single symmetrical peak, indicating that EPS was a homogeneous polysaccharide ( Figure 2A). The monosaccharide components of EPS were determined by Thin layer chromatography (TLC) ( Figure 2B). EPS hydrolysate was expressed at the same point with the glucose used as the standard, so the results indicated that EPS was mainly composed of glucose. We conducted Benedict's test to confirm that EPS is primarily composed of glucose. EPS hydrolysate changed the color of the reagent into orange-red ( Figure 2C). Additionally, the color of the reagent was changed into green by EPS. The characteristic structure of EPS was analyzed using Fourier-transform infrared spectroscopy (FTIR). The result of FTIR showed a complex pattern of peaks from 3500 to 500 cm −1 ( Figure 2D). The peaks indicated the characteristic group of glucose such as the presence of O-H groups at 3307.31 cm −1 , a weak C-H stretching peak of methyl groups at 2935.1 cm −1 , and C=O stretching at 1648.88 cm −1 [25]. Additionally, the strongest absorption band, 1032.58 cm −1 , was assigned to C-O bond and O-H bond, identifying the presence of polysaccharides [26]. The bands at 911.98 and 812.28 cm −1 corresponded to the side-group of carbohydrates [27]. Thus, the results showed that EPS had the absorption peaks of polysaccharides mainly composed of glucose. To measure the molecular weight of EPS, EPS was analyzed through Gel Permeation Chromatography (GPC) with pullulan standards ( Figure 2E). When the molecular weight is calculated by the chromatography data system, EPS has a number average molecular weight (Mn) of 1.84 × 10 4 Da, a weight average molecular weight (Mw) of 7.57 × 10 4 Da, a size average molecular weight (Mz) of 3.74 × 10 5 Da, and a polydispersity index (PDI) (Mw/Mn) of 4.12. These results indicated that EPS was a homogeneous polysaccharide mainly composed of glucose. The result of FTIR showed a complex pattern of peaks from 3500 to 500 cm −1 ( Figure 2D). The peaks indicated the characteristic group of glucose such as the presence of O-H groups at 3307.31 cm −1 , a weak C-H stretching peak of methyl groups at 2935.1 cm −1 , and C=O stretching at 1648.88 cm −1 [25]. Additionally, the strongest absorption band, 1032.58 cm −1 , was assigned to C-O bond and O-H bond, identifying the presence of polysaccharides [26]. The bands at 911.98 and 812.28 cm −1 corresponded to the side-group of carbohydrates [27]. Thus, the results showed that EPS had the absorption peaks of polysaccharides mainly composed of glucose. To measure the molecular weight of EPS, EPS was analyzed through Gel Permeation Chromatography (GPC) with pullulan standards ( Figure 2E). When the molecular weight is calculated by the chromatography data system, EPS has a number average molecular weight (Mn) of 1.84 × 10 4 Da, a weight average molecular weight (Mw) of 7.57 × 10 4 Da, a size average molecular weight (Mz) of 3.74 × 10 5 Da, and a polydispersity index (PDI) (Mw/Mn) of 4.12. These results indicated that EPS was a homogeneous polysaccharide mainly composed of glucose.

EPS Isolated from L-14 Alleviated Morphological Changes Induced by LPS within Mouse Macrophages
To confirm whether EPS alleviated the morphological changes induced by LPS, cells were pretreated with EPS for 6 h, and then, the morphological changes were stimulated by LPS for 18 h.

EPS Isolated from L-14 Alleviated Morphological Changes Induced by LPS within Mouse Macrophages
To confirm whether EPS alleviated the morphological changes induced by LPS, cells were pretreated with EPS for 6 h, and then, the morphological changes were stimulated by LPS for 18 h. Crystal violet staining showed that EPS treatment could alleviate the alteration of cell morphology in LPS-treated cells ( Figure 3A). Furthermore, when RAW 264.7 cells were treated with a higher concentration of EPS for 1 day, cell viability remained unaffected ( Figure 3B). These results indicated that EPS inhibited LPS-induced morphological changes in mouse macrophages without impacting cell viability. Crystal violet staining showed that EPS treatment could alleviate the alteration of cell morphology in LPS-treated cells ( Figure 3A). Furthermore, when RAW 264.7 cells were treated with a higher concentration of EPS for 1 day, cell viability remained unaffected ( Figure 3B). These results indicated that EPS inhibited LPS-induced morphological changes in mouse macrophages without impacting cell viability.

LPS-Induced Inflammatory Response Was Inhibited by EPS Pretreatment
To confirm whether the isolated EPS inhibited the inflammatory response resulting from LPS stimulation, we quantified the proinflammatory cytokines produced by RAW 264.7 cells precultured with EPS. EPS pretreatment attenuated IL-6, TNF-α, and IL-1β levels in culture media, and in particular, IL-1β was decreased similar to the expression levels of the control ( Figure 4A). Furthermore, the expression of COX-2 and inducible nitric oxide synthase (iNOS), known as major mediators of inflammation, was analyzed through Western blot. While the expressions of COX-2 and iNOS proteins were increased following LPS treatment, these expression levels were suppressed in EPS-pretreated RAW 264.7 cells ( Figure 4B). Consistent with these results, immunofluorescence (IF) assay also confirmed that the expression of LPS-induced iNOS was decreased in EPS-pretreated RAW 264.7 cells ( Figure 4C). Taken together, these results indicate that EPS purified from L-14 exhibited suppression upon the LPS-stimulated inflammatory response.

LPS-Induced Inflammatory Response Was Inhibited by EPS Pretreatment
To confirm whether the isolated EPS inhibited the inflammatory response resulting from LPS stimulation, we quantified the proinflammatory cytokines produced by RAW 264.7 cells precultured with EPS. EPS pretreatment attenuated IL-6, TNF-α, and IL-1β levels in culture media, and in particular, IL-1β was decreased similar to the expression levels of the control ( Figure 4A). Furthermore, the expression of COX-2 and inducible nitric oxide synthase (iNOS), known as major mediators of inflammation, was analyzed through Western blot. While the expressions of COX-2 and iNOS proteins were increased following LPS treatment, these expression levels were suppressed in EPS-pretreated RAW 264.7 cells ( Figure 4B). Consistent with these results, immunofluorescence (IF) assay also confirmed that the expression of LPS-induced iNOS was decreased in EPS-pretreated RAW 264.7 cells ( Figure 4C). Taken together, these results indicate that EPS purified from L-14 exhibited suppression upon the LPS-stimulated inflammatory response.

EPS Inhibited Nuclear Translocation of NF-κB Induced by LPS
To examine whether EPS inhibited phosphorylation and translocation to the nucleus of NF-κB following induction by LPS, the inflammatory response was stimulated by LPS in RAW 264.7 cells pretreated with EPS followed by an analysis of the expression level of NF-κB and localization of its phosphorylated form. The p-NF-κB/NF-κB ratio was then decreased by EPS pretreatment ( Figure  5A). EPS itself did not promote the phosphorylation of NF-κB. As shown in Figure 5B, EPS suppressed LPS-induced nuclear translocation of NF-κB at all concentrations. Consistently, while translocation of NF-κB into the nucleus was induced by LPS, this was reduced by pretreatment of EPS in RAW 264.7 cells ( Figure 5C).

EPS Inhibited Nuclear Translocation of NF-κB Induced by LPS
To examine whether EPS inhibited phosphorylation and translocation to the nucleus of NF-κB following induction by LPS, the inflammatory response was stimulated by LPS in RAW 264.7 cells pretreated with EPS followed by an analysis of the expression level of NF-κB and localization of its phosphorylated form. The p-NF-κB/NF-κB ratio was then decreased by EPS pretreatment ( Figure 5A). EPS itself did not promote the phosphorylation of NF-κB. As shown in Figure 5B, EPS suppressed LPS-induced nuclear translocation of NF-κB at all concentrations. Consistently, while translocation of NF-κB into the nucleus was induced by LPS, this was reduced by pretreatment of EPS in RAW 264.7 cells ( Figure 5C).

EPS-Repressed Inflammatory Response via Regulation of MAPK and Nuclear Factor E2-Related Factor 2 (NRF2)/Heme Oxygenase-1 (HO-1) Pathways in RAW 264.7 Cells
The MAPK and NRF2/HO-1 pathways are identified as major regulators of the inflammatory response in mouse macrophages. To determine whether EPS has any effects on the MAPK pathway, phosphorylation of MAPK family proteins (JNK, ERK, and p38) was analyzed. EPS significantly inhibited the LPS-induced phosphorylation of JNK and ERK even at 100 μg/mL of EPS ( Figure 6A). Phosphorylation of p38 resulting from LPS was suppressed in the cells treated with EPS at a concentration of 200 μg/mL. To confirm that the anti-inflammatory effects of EPS were mediated through the NRF2/HO-1 pathway, the protein expression levels of known anti-oxidant markers were identified. The expression levels of both HO-1 and NFR2 were then increased with or without LPS ( Figure 6B). Consistent with the previous results, the translocation of NRF2 into the nucleus was also The MAPK and NRF2/HO-1 pathways are identified as major regulators of the inflammatory response in mouse macrophages. To determine whether EPS has any effects on the MAPK pathway, phosphorylation of MAPK family proteins (JNK, ERK, and p38) was analyzed. EPS significantly inhibited the LPS-induced phosphorylation of JNK and ERK even at 100 µg/mL of EPS ( Figure 6A). Phosphorylation of p38 resulting from LPS was suppressed in the cells treated with EPS at a concentration of 200 µg/mL. To confirm that the anti-inflammatory effects of EPS were mediated through the NRF2/HO-1 pathway, the protein expression levels of known anti-oxidant markers were identified. The expression levels of both HO-1 and NFR2 were then increased with or without LPS ( Figure 6B). Consistent with the previous results, the translocation of NRF2 into the nucleus was also increased following EPS exposure ( Figure 6C). In summary, EPS inhibited the phosphorylation of MAPK family proteins and enhanced the expression of NRF2/HO-1 in LPS-induced RAW 264.7 cells. increased following EPS exposure ( Figure 6C). In summary, EPS inhibited the phosphorylation of MAPK family proteins and enhanced the expression of NRF2/HO-1 in LPS-induced RAW 264.7 cells.

EPS Inhibits the Inflammatory Response by Suppressing the Interaction between LPS and TLR4
To examine whether EPS suppressed the inflammatory response via TLR4, the expression level of TLR4 was analyzed following EPS and LPS treatment of RAW 264.7 cells. As a result, TLR4 upregulated by LPS was reduced by EPS at all concentrations ( Figure 7A). In addition, the manner in which EPS interacted with TLR4 was confirmed using TAK-242, which has been determined to block the TLR4 pathway. Interestingly, when RAW 264.7 cells were treated with EPS only, the protein expression of TLR4 was more repressed than that of the no treatment control group ( Figure 7B). EPS also inhibited the expression levels of TLR4 and MyD88 in LPS-induced groups, similar to that observed in the TAK-242-treated group. The expression of COX-2 was inhibited by TAK-242 and was inhibited by EPS in the same way. Consistently, EPS significantly reduced the expression of the secreted cytokines IL-1β, IL-6, and TNF-α in the media as much as TAK-242 downregulated them ( Figure 7C). The results suggested that EPS exhibited anti-inflammatory effects through TLR4 in LPStreated RAW 264.7 cells.

EPS Inhibits the Inflammatory Response by Suppressing the Interaction between LPS and TLR4
To examine whether EPS suppressed the inflammatory response via TLR4, the expression level of TLR4 was analyzed following EPS and LPS treatment of RAW 264.7 cells. As a result, TLR4 upregulated by LPS was reduced by EPS at all concentrations ( Figure 7A). In addition, the manner in which EPS interacted with TLR4 was confirmed using TAK-242, which has been determined to block the TLR4 pathway. Interestingly, when RAW 264.7 cells were treated with EPS only, the protein expression of TLR4 was more repressed than that of the no treatment control group ( Figure 7B). EPS also inhibited the expression levels of TLR4 and MyD88 in LPS-induced groups, similar to that observed in the TAK-242-treated group. The expression of COX-2 was inhibited by TAK-242 and was inhibited by EPS in the same way. Consistently, EPS significantly reduced the expression of the secreted cytokines IL-1β, IL-6, and TNF-α in the media as much as TAK-242 downregulated them ( Figure 7C). The results suggested that EPS exhibited anti-inflammatory effects through TLR4 in LPS-treated RAW 264.7 cells.

Discussion
Inflammatory reactions are considered a common biological response to pathogens, which occurs in all tissues and organs [28]. Inflammation is deemed essential for the elimination of harmful stimuli and damaged cells and initiation of tissue repair involving immune cells and molecular mediators. However, an uncontrolled or excessive inflammatory response can contribute to chronic inflammatory diseases and, in some instances, even death [29,30]. Thus, a number of attempts have been performed to develop therapeutics to treat diseases caused by unregulated inflammation [31]. Recently, it has been reported that postbiotic compounds are capable of regulating the immune responses of various animal models [32]. For example, EPS produced by Lactobacillus delbrueckii inhibited the inflammatory response induced by E. coli via the IκB pathway in porcine intestinal epithelial cells [33]. However, the receptor interacting with EPS and related mechanisms remain poorly understood.
When LPS binds to TLR4, the interaction can induce an inflammatory response and oxidative stress by NF-κB and MAPK signaling pathway in the host cells [7]. TLR4 signaling is divided into two pathways, that is, the MyD88-dependent pathway and TIR-domain-containing adapter-inducing interferon-β (TRIF)-dependent pathway, of which the mutual interaction generates a complicated inflammation response [34]. TNF-α expression induced by LPS is regulated through both MyD88 and TRIF pathways, whereas NF-κB is primarily activated through a MyD88-dependent pathway [35]. Our results have shown that EPS has inhibited the expression of TNF-α and NF-κB following their activation by LPS ( Figures 4A and 5). In some recent research, it was shown that LPS accumulated in the brain neurons of patients with Alzheimer's disease and inhibited the efficient readout of neuronal genetic information for the homeostasis of brain cell function by contributing to inflammatory

Discussion
Inflammatory reactions are considered a common biological response to pathogens, which occurs in all tissues and organs [28]. Inflammation is deemed essential for the elimination of harmful stimuli and damaged cells and initiation of tissue repair involving immune cells and molecular mediators. However, an uncontrolled or excessive inflammatory response can contribute to chronic inflammatory diseases and, in some instances, even death [29,30]. Thus, a number of attempts have been performed to develop therapeutics to treat diseases caused by unregulated inflammation [31]. Recently, it has been reported that postbiotic compounds are capable of regulating the immune responses of various animal models [32]. For example, EPS produced by Lactobacillus delbrueckii inhibited the inflammatory response induced by E. coli via the IκB pathway in porcine intestinal epithelial cells [33]. However, the receptor interacting with EPS and related mechanisms remain poorly understood.
When LPS binds to TLR4, the interaction can induce an inflammatory response and oxidative stress by NF-κB and MAPK signaling pathway in the host cells [7]. TLR4 signaling is divided into two pathways, that is, the MyD88-dependent pathway and TIR-domain-containing adapter-inducing interferon-β (TRIF)-dependent pathway, of which the mutual interaction generates a complicated inflammation response [34]. TNF-α expression induced by LPS is regulated through both MyD88 and TRIF pathways, whereas NF-κB is primarily activated through a MyD88-dependent pathway [35]. Our results have shown that EPS has inhibited the expression of TNF-α and NF-κB following their activation by LPS (Figures 4A and 5). In some recent research, it was shown that LPS accumulated in the brain neurons of patients with Alzheimer's disease and inhibited the efficient readout of neuronal genetic information for the homeostasis of brain cell function by contributing to inflammatory degeneration in human neuronal-glial cells [36]. Rathinam et al. showed that TLR4-deficient mice did not develop an immune response following injections of LPS at high concentrations, which only suggests that the inflammatory reaction caused by LPS may be completely regulated through TLR4 pathway [37]. EPS obtained from LAB could be divided into homopolysaccharide (HoPS) composed of the same monosaccharides and heteropolysaccharide consists of various monosaccharides [16]. In particular, HoPS is polymerized from monosaccharides such as glucose or fructose by glycosyl hydrolase activity and released out from the cell [38]. HoPS is produced through either intracellular synthesis, which undergoes several metabolic pathways or extracellular synthesis, which is released immediately after polymerization. Therefore, HoPS has different ligands depending on the methods it is made. These complex structural properties contribute to the biological function, characteristics, or beneficial effects of EPS. EPS isolated L-14 was identified as HoPS mainly composed of glucose ( Figure 2B-D). The PDI of EPS was calculated as 4.12, and EPS changed the color of the Benedict solution to green, indicating that EPS contained a small amount of glucose. (Figure 2C,E). These results confirmed that EPS is a broad polydisperse polysaccharide composed of monosaccharides of glucose. Interestingly, our results showed that EPS isolated from L-14 significantly inhibited the expression of proinflammatory markers-including IL-6, TNF-α, IL-1β, and COX-2-induced by LPS, indicating that EPS suppressed the TLR4 pathway (Figures 4 and 7A). In addition, our findings have confirmed that EPS inhibited the activation of TLR4 by LPS, as demonstrated by TAK-242 treatment ( Figure 7B,C). When cells were treated with both EPS and TAK-242, TLR4 and proinflammatory mediators were not more repressed than when cells were treated with either agent independently. These results suggested the possibility that EPS inhibits the inflammatory response via a mechanism similar to that of TAK-242. TLR4 directly binds to not only LPS but also products of gram-positive bacteria such as EPS and lipoteichoic acid. Recently, it was reported that the EPS produced by Bifidobacterium animalis was able to interact with TLR4 of intestinal epithelial cells [39]. Nevertheless, it is necessary to elaborate in detail what structure of EPS from L-14 has and whether the ligand of EPS directly binds to TLR4 like TAK-242. Anti-cytokine therapy has been widely used to treat immune diseases in the last decade [40]. Such approved drugs include inhibitors of TNF-α (e.g., etanercept and adalimumab), IL-6 (e.g., tocilizumab), and IL-1β (e.g., canakinumab) signaling for immune diseases such as rheumatoid arthritis and Crohn's disease [41]. Surprisingly, proinflammatory cytokines are also found to be highly expressed in major depression disorder (MDD) patients [42]; in particular, TNF-α stimulates the development of MDD by changing the function and structure of the brain [43]. In randomized trials for MDD treatment, depressive symptoms were shown to decrease by up to 43.8% after taking anti-TNF-α agents (i.e., etanercept, adalimumab, and golimumab) [44]. Furthermore, IL-1β is known as the major cytokine contributing to cardiac ischemia reperfusion injury due to activation through the inflammasome. Cytokines present in the myocardium, including IL-1β, TNF-α, and IL-6, have even resulted in life-threatening ventricular arrhythmias through modulation of potassium and calcium channels [45]. Administration of anti-IL-1β monoclonal antibody reduced the relative risk of major adverse cardiovascular events by up to 25%, in addition to the 31% decline in cardiovascular and overall mortality in patients with a history of myocardial infarction [46]. However, injection of therapeutic monoclonal antibody has commonly resulted in adverse reactions, including infections and reactions at the injection site [47]. Our experiment showed that EPS can effectively reduce the secretion of proinflammatory cytokines without cytotoxicity in macrophages (Figures 3B and 4). The inflammatory response of macrophages can promote the development of the innate and adaptive immune response by complex interactions with other immune cells, including natural killer cells and dendritic cells [48]. EPS could be used to inhibit inflammatory reactions in macrophages and further treat diseases caused by unregulated inflammatory reactions through oral intake.
EPS has been determined to upregulate the expression of NRF2/HO-1 and consequently decreased iNOS, a known producer of nitric oxide (NO), one of reactive oxygen species (ROS), in LPS-induced macrophages ( Figure 4B,C and Figure 6B,C). Oxidative stress often results in the damage of DNA and proteins, mitochondrial dysfunction, and apoptosis, which can lead to age-related diseases such as chronic inflammatory diseases and cancer [49]. Additionally, excessive ROS causing oxidative stress in cells are considered as risks and enhancer factors for chronic inflammatory diseases [50]. The activation of the anti-oxidant molecules NRF2/HO-1 exhibited a clinical benefit through anti-oxidant effects in animal models with rheumatoid arthritis [51]. Recently, it has been reported that symptoms of neurodegenerative diseases deteriorated when deficiency of NRF2 increased neuroinflammation and oxidative stress in mice [52]. Additionally, it was reported that oxidative stress can be provoked by activating the MAPK pathway, since the members of the ROS and MAPK families can positively regulate each other [53]. For example, hydrogen peroxide treatment increases the expression of genes that can activate the MAPK pathway; likewise, oxidative stress was suppressed via treatment with the MAPK inhibitors PD98059 (ERK inhibitor) and SB203580 (p38 inhibitor) [54]. Taken together, the regulation of the NRF2/HO-1 and MAPK pathways has been determined to suppress ROS production, which leads to the reduction of the oxidative stress response. Although the inhibitory activities of EPS were not observed in a dose-dependent manner, phosphorylation of JNK, ERK, and p38 was decreased by EPS ( Figure 6A). Consistently, the expression of iNOS has reportedly decreased at the protein level ( Figure 4B,C). Interestingly, even though p38 was phosphorylated by EPS itself, EPS did not significantly affect the expression of iNOS ( Figure 4B,C and Figure 6A). Although the NO level was not quantified and it was not determined how iNOS and the MAPK pathway regulated each other, it appears indisputable that EPS retains the ability to reduce oxidative stress. Therefore, EPS could be a potential substance to manage ROS levels, and, consequently, prevent and treat the diseases caused by oxidative stress.
TLR4 has been applied as a target for immunopharmacological control of infection from pathogenic bacteria and even viruses [55]. Especially, viral infection can lead to a cytokine storm, which develops via overexpressed proinflammatory mediators, making the immune cells unable to prevent cytokine production [56]. A recent study has confirmed that the coronavirus causing COVID-19 stimulates a TLR4-mediated inflammatory response similar to the pathogenic process of bacterial sepsis [57]. Furthermore, it has been reported that the innate immune response during Ebola virus infection begins when the viral glycoprotein binds to TLR4; subsequently, NF-κB and MAPK signaling pathways are activated [58]. Shirey et al. confirmed the possibility for TLR4 antagonists (e.g., eritoran) to be used as novel therapeutics for the influenza virus-induced cytokine storm [59]. Liu Shen Wan, a known traditional medicine of China, even inhibited the proliferation of the virus as well as expression of cytokines by suppressing TLR4 [60]. Interestingly, our results have shown that EPS itself downregulated TLR4 and MyD88 compared with the control group ( Figure 7B); this suggests that it can act as an inhibitor of TLR4. Thus, EPS could be used to prevent and treat the diseases caused by uncontrolled inflammatory response associated with virus infections. It has also been shown that TLR4 is involved in the maintenance of host homeostasis [61]. Knockdown of TLR4 ameliorates insulin resistances and glucose tolerance, suggesting that TLR4 is a key therapeutic target for metabolic disorders [62]. Withaferin A decreases the gene expression of TLR4 and COX-2, which protects high-fat diet-induced mice against metabolic disorders such as glucose tolerance, insulin resistance, and oxidative stress [63]. Expression of COX-2 in the EPS treatment group was more downregulated than that of the control group by suppressing TLR4, regardless of the presence of LPS ( Figure 7B). It has already been reported that curcumin, known as a TLR4 inhibitor, can be a candidate for the treatment of metabolic syndrome and type II diabetes mellitus through a clinical trial test [64]. Therefore, EPS could potentially be used to treat metabolic diseases as well as diseases resulting from bacterial and viral infections. However, it is essential to confirm the effect EPS has on normal cells and whether EPS can exhibit anti-inflammatory effect in vivo.
In conclusion, EPS isolated from L. plantarum L-14 was polysaccharide mainly composed of glucose and inhibited proinflammatory mediators such as those of the NF-κB and MAPK pathways by suppressing TLR4 and MyD88 signaling (Figure 8). This suggests that EPS could be an attractive candidate for natural product-derived medicine in regulating acute or chronic inflammatory reactions. It remains unclear how EPS itself decreases the expression of TLR4 or whether EPS regulates them directly or indirectly. Follow-up study is required to elaborate the clear interaction between EPS and TLR4.

Figure 8.
When LPS binds to TLR4 in the cell membrane, the resulting complex can lead to the activation of adaptor proteins including MyD88 and TIR-domain-containing adapter-inducing interferon-β (TRIF). NF-κB is phosphorylated and translocated into the nucleus by adaptor proteins. MyD88 and TRIF phosphorylate members of the MAPK pathway, and the MAPK family protein translocates transcription factors related to the inflammatory response into the nucleus. These translocated factors upregulate the expression of proinflammatory cytokines and COX-2. However, EPS could inhibit the LPS-induced inflammatory response by downregulating TLR4. EPS also repressed the expression of iNOS, which led to oxidative stress by upregulating the NRF2/HO-1 pathways. Nevertheless, more research is required to elucidate how EPS regulates the expression of TLR4.

L-14 Culture and Exopolysaccharide Purification
The L-14 strain (KTCT13497BP), which was purchased from NeoRegen Biotech (Suwon, Gyeonggi-do, Korea), was cultured at 30 °C for 18 h in Man, Rogosa and Sharpe (MRS; Hardy Diagnostics, Santa Maria, CA, USA) broth, which contained 2% dextrose, 1% peptic digest of animal tissue, 1% beef extract, 0.5% yeast extract, 0.5% sodium acetate, 0.2% disodium phosphate, 0.2% ammonium citrate, 0.1% polysorbate 80, 0.01% magnesium sulfate, and 0.005% manganese sulfate. EPS was purified using the ethanol precipitation method as previously described [65]. In summary, L-14 culture media was separated via centrifugation at 10,000× g for 20 min. Media supernatant was then isolated, and trichloroacetic acid was added to denature proteins in the L-14-cultured media at 37 °C for 1 h. Denatured proteins were isolated from media by centrifugation at 10,000× g for 20 min, followed by mixing with absolute ethanol. The separated precipitates were dialyzed with the distilled water (DW) at 4 °C for 24~48 h to fully remove the components of media and other substances. The dialyzed solution was then lyophilized to obtain EPS, which was resuspended in DW for subsequent experiments and stored −80 °C.

L-14 Culture and Exopolysaccharide Purification
The L-14 strain (KTCT13497BP), which was purchased from NeoRegen Biotech (Suwon, Gyeonggi-do, Korea), was cultured at 30 • C for 18 h in Man, Rogosa and Sharpe (MRS; Hardy Diagnostics, Santa Maria, CA, USA) broth, which contained 2% dextrose, 1% peptic digest of animal tissue, 1% beef extract, 0.5% yeast extract, 0.5% sodium acetate, 0.2% disodium phosphate, 0.2% ammonium citrate, 0.1% polysorbate 80, 0.01% magnesium sulfate, and 0.005% manganese sulfate. EPS was purified using the ethanol precipitation method as previously described [65]. In summary, L-14 culture media was separated via centrifugation at 10,000× g for 20 min. Media supernatant was then isolated, and trichloroacetic acid was added to denature proteins in the L-14-cultured media at 37 • C for 1 h. Denatured proteins were isolated from media by centrifugation at 10,000× g for 20 min, followed by mixing with absolute ethanol. The separated precipitates were dialyzed with the distilled water (DW) at 4 • C for 24~48 h to fully remove the components of media and other substances. The dialyzed solution was then lyophilized to obtain EPS, which was resuspended in DW for subsequent experiments and stored −80 • C.

FPLC
To identify EPS as a homogeneous polysaccharide, EPS (30 mg/mL) was purified by size exclusion chromatography on a HiLoad ® 16/600 Superdex 200 pg column (GE Healthcare, Chicago, IL, USA) with phosphate-buffered saline (PBS), and it was analyzed via the ÄKTA FPLC system (GE Healthcare).

TLC and Benedict's Test
To identify the monosaccharide composition of EPS through TLC, 10 mg of EPS was hydrolyzed with 1 mL sulfuric acid (2 N) at 100 • C for 4 h. The residual sulfuric acid was neutralized with the enough BaCO3 for 12 h. After EPS hydrolysate was adjusted to PH 7, it was lyophilized for the analysis.
EPS hydrolysate was treated on TLC Silica gel (Merck, Darmstadt, Germany) and migrated with the buffer composed with n-butanol:methanol:25% ammonia solution:DW (5:4:2:1). To visualize the composition of EPS, the gel was soaked with aniline-diphenylamine reagent and baked in the oven at 110 • C for 5 min.
To conduct Benedict's test, EPS and EPS hydrolysate were mixed with the same quantify of Benedict's reagent (BIOZOA Biological Supply, Seoul, Korea) and then heated in boiling water.

FTIR and GPC
To confirm the structural characteristics of EPS, EPS was analyzed using TENSOR27 FTIR (Bruker, Billerica, MA, USA) in the absorption range from 4000 to 500 cm −1 by National Center for Inter-University Research Facilities of Seoul National University.
To determine the molecular weight of EPS, GPC analysis was performed using Dionex HPLC Ultimate3000 RI System (Thermo Scientific, Waltham, MA, USA) with 120 Å, 500 Å, and 1000 Å columns (Waters, Milford, MA, USA) at 40 • C by National Instrumentation Center for Environmental Management of Seoul National University. The data of the experiment was calibrated with pullulan and processed with the chromatography data system (Chromeleon 6.8 Extention-pak). EPS was eluted using Sodium azide 0.1 M in water and operated at a flow rate of 1 mL/min.

Cell Culture
The mouse macrophage cell line RAW 264.7 was obtained from the American Type Culture Collection. The cells were cultured in Dulbecco's modified Eagle's media (WELGENE, Daegu, Korea), which contained 10% fetal bovine serum (HyClone, Logan, UT, USA) and 1% penicillin/streptomycin (Gibco, Grand Island, NY, USA). The cells were cultured at 37 • C in an incubator with a humidified atmosphere of 5% CO 2 .

Cell Viability Assay
RAW 264.7 cells were seeded at a density of 2.0 × 10 3 cells per well in 96-well plates. A day after, media was replaced with L-14 media and cultured for another 6 h. The media containing L-14 was then replaced with fresh media containing LPS to induce inflammatory response at the indicated concentrations for 6 h. Cell viability was confirmed using Quanti-Max WST-8 cell viability kit (BIOMAX, Seoul, Korea). To determine the effect of EPS on cell viability, the seeded cells were incubated in media containing EPS at various concentrations for 1 day. Cell viability was verified by the same kit.

ELISA
RAW 264.7 cells were seeded into 12-well plates at a density of 2.0 × 10 5 cells per well. The media was then replaced with L-14-inoculated media at a concentration of 1.0 × 10 6 CFU/mL and maintained for 6 h. The media was then removed, and cells were washed thoroughly three times with DMEM. To induce inflammation, the washed cells were cultured in media containing LPS (1 µg/mL) and maintained for 6 h. The culture media obtained from each well was centrifuged at 10,000× g for 3 min, and the supernatants were collected. Cytokines were quantified by ELISA MAX™ Deluxe Set (BioLegend) according to the manufacturer's recommendations. To examine whether pretreatment of EPS decreased the induction of cytokines by LPS, RAW 264.7 cells were seeded into 12-well plates for 24 h. The cells were pretreated with EPS for 6 h, and then the cultured media was replaced with fresh media containing LPS for 18 h to induce an inflammatory response. The cytokines in the culture media were quantified as described above.

Crystal Violet Staining
RAW 264.7 cells were seeded into 12-well plates and incubated for 1 day. To examine whether EPS pretreatment has affected the morphology of cells and inhibited the morphological changes induced by LPS, cells were treated with EPS for 6 h, and the cultured media was replaced with the fresh media containing LPS (1 µg/mL) for 18 h. Then, the cells were washed with PBS and stained with crystal violet solution (Sigma-Aldrich, Saint Louis, MO, USA). The morphological changes were determined using an EVOS CL Core microscope (Life Technologies, Carlsbad, CA, USA) at 100× magnification.

Western Blot
The proteins were isolated from RAW 264.7 cells treated with LPS using Cell Culture Lysis 1× Reagent (Promega, Fitchburg, WI, USA) with a protease inhibitor cocktail and phosphatase inhibitor cocktail. The cytoplasmic and nuclear proteins were obtained using ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit (Abbkine, Wuhan, China) according to the manufacturer's instructions. The total protein concentration was quantified by Pierce™ BCA Protein Assay Kit (Thermo Scientific, Waltham, MA, USA). The denatured protein was then separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane. After blocking for 1 h at room temperature (RT), the membrane was incubated in skim milk containing the proper primary antibodies (1:1000) overnight at 4 • C. The membrane was washed using tris-buffered saline with 0.1% Tween 20 (Sigma) and was further incubated in skim milk containing secondary antibodies (1:2000) for 1 h at RT. The protein signals were detected using the ECL Western Blot Substrate (Daeil Lab Service, Seoul, Korea).

IF Assay
RAW 264.7 cells were seeded at a density of 1.0 × 10 6 cells per well in 6-well plates and were incubated overnight. EPS-pretreated cells were cultured in fresh media containing LPS and harvested by scraping. After fixation with 4% paraformaldehyde and permeabilization by 0.1% Triton X-100 (Sigma), the cells were blocked with 3% bovine serum albumin (BSA, Bovogen, East Keilor, Australia) for 1 h; then, they were incubated with PE-conjugated iNOS antibody. The washed cells were mounted with ProLong™ Glass Antifade Mountant with NucBlue™ Stain (Invitrogen).
To visualize the translocation of NF-κB, RAW 264.7 cells were pretreated with EPS for 2 h, and the inflammatory response was stimulated by LPS (1 µg/mL) for 1 h. The isolated cells were prepared via the same method. After blocking for 1 h, cells were incubated with NF-κB antibody (1:200) for 18 h at 4 • C. The cells were then washed thoroughly and incubated in 3% BSA with Alexa Fluor 488-conjugated secondary antibodies for 30 min at RT. Cells were mounted using the same stain. All slides were analyzed using LSM 800 confocal laser scanning microscope 293 (Carl Zeiss, Oberkochen, Germany).

Statistics
In this study, all data were obtained from three independent experiments and presented with mean ± standard deviation (SD). Statistical analysis was determined using unpaired ANOVA, and significance was defined as * p < 0.05, ** p < 0.01, and *** p < 0.001.

Conflicts of Interest:
The authors declare no conflict of interest. The founding sponsors had no role in the design of this study; in the collection, analysis, or interpretation of the data; in the writing of the manuscript; or in the decision to publish the results. NeoRegen Biotech Co., Ltd. supported the salary for J.L, S.P., O.-H.K., and J.S.