Developmental Roles of FUSE Binding Protein 1 (Fubp1) in Tooth Morphogenesis
Abstract
:1. Introduction
2. Results
2.1. Expression Pattern and Functional Evaluation of Fubp1
2.2. Altered Expression Patterns of Signalling Molecules by Fubp1 Knock-Down
2.3. Fubp1 in Tooth Morphogenesis
3. Discussion
4. Material and Methods
4.1. Animals
4.2. In Situ Hybridization
4.3. siRNA Transfection, In Vitro Organ Cultivation and Kidney Transplantation
4.4. Histology, Immunohistochemistry and Western Blotting
4.5. Quantitative PCR (qPCR)
4.6. Ground Section and Scanning Electron Microscopy (SEM)
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gene | Accession | Primer Sequence | Product Size (bp) | Remarks | |
---|---|---|---|---|---|
Fubp1 | NM_001355372.1 | Forward | GCATCAGCAGCAAAGCAGAT | 419 | Probe synthesis |
Reverse | CAGCACCAGTGTTTTGAGGC | ||||
Forward | TGCAGCAAAAATTGGGGGTG | 173 | qPCR | ||
Reverse | ATCTGCTTTGCTGCTGATGC | ||||
Ambn | NM_001303431.1 | Forward | TTCTTGCTTTCCCCAATGAC | 234 | Amelogenesis |
Reverse | GGTGCACTTTGTTTCCAGGT | ||||
Amelx | XM_017348358.1 | Forward | GCAGCCGTATCCTTCCTATGGTT | 120 | Amelogenesis |
Reverse | GGAAGGTGGTGATGAGGCTGAA | ||||
Axin2 | BC057338.1 | Forward | TGAAGAAGAGGAGTGGACGT | 115 | Wnt signalling |
Reverse | AGCTGTTTCCGTGGATCTCA | ||||
β-catenin | NM_007614.3 | Forward | TGACCTGATGGAGTTGGACA | 104 | Wnt signalling |
Reverse | TGGCACCAGAATGGATTCCA | ||||
Bmp2 | NM_007553.3 | Forward | AAGTGGCCCATTTAGAGGAG | 104 | Bmp signalling |
Reverse | CCATGGCCTTATCTGTGACC | ||||
Bmp4 | NM_007554.3 | Forward | ACCTCAAGGGAGTGGAGATT | 113 | Bmp signalling |
Reverse | GATGCTTGGGACTACGTTTG | ||||
Dspp | NM_010080.3 | Forward | GTGGGGTTGCTACACATGAAAC | 169 | Dentinogenesis |
Reverse | CCATCACCAGAGCCTGTATCTTC | ||||
Fgf4 | NM_010202.5 | Forward | TCGCCTACCATGAAGGTAAC | 114 | Fgf signalling |
Reverse | TCTCCATCGAGAGAAAGTGC | ||||
Lef1 | NM_010703.4 | Forward | ACAGCGACGAGCACTTTTCT | 82 | Shh Signalling |
Reverse | TGTCTGGACATGCCTTGCTT | ||||
Rock1 | NM_009071.2 | Forward | GGTATCGTCACAAGTAGCAGCATC | 140 | Wnt signalling |
Reverse | TAAACCAGGGCATCCAATCCA | ||||
Shh | NM_009170.3 | Forward | CCAAAGCTCACATCCACTGT | 131 | Shh Signalling |
Reverse | GGGACGTAAGTCCTTCACCA | ||||
Hprt | NM_013556.1 | Forward | CCTAAGATGATCGCAAGTTG | 86 | Internal standard |
Reverse | CCACAGGGACTAGAACACCTGCTAA |
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Aryal, Y.P.; Neupane, S.; Kim, T.-Y.; Lee, E.-S.; Pokhrel, N.K.; Yeon, C.-Y.; Kim, J.-Y.; An, C.-H.; An, S.-Y.; Park, E.-K.; et al. Developmental Roles of FUSE Binding Protein 1 (Fubp1) in Tooth Morphogenesis. Int. J. Mol. Sci. 2020, 21, 8079. https://doi.org/10.3390/ijms21218079
Aryal YP, Neupane S, Kim T-Y, Lee E-S, Pokhrel NK, Yeon C-Y, Kim J-Y, An C-H, An S-Y, Park E-K, et al. Developmental Roles of FUSE Binding Protein 1 (Fubp1) in Tooth Morphogenesis. International Journal of Molecular Sciences. 2020; 21(21):8079. https://doi.org/10.3390/ijms21218079
Chicago/Turabian StyleAryal, Yam Prasad, Sanjiv Neupane, Tae-Young Kim, Eui-Seon Lee, Nitin Kumar Pokhrel, Chang-Yeol Yeon, Ji-Youn Kim, Chang-Hyeon An, Seo-Young An, Eui-Kyun Park, and et al. 2020. "Developmental Roles of FUSE Binding Protein 1 (Fubp1) in Tooth Morphogenesis" International Journal of Molecular Sciences 21, no. 21: 8079. https://doi.org/10.3390/ijms21218079