No Role of Osteocytic Osteolysis in the Development and Recovery of the Bone Phenotype Induced by Severe Secondary Hyperparathyroidism in Vitamin D Receptor Deficient Mice
Abstract
:1. Introduction
2. Results
2.1. Strong Correlations of Serum PTH, ALP, and Serum Calcium Levels with Bone Matrix Mineralization, but Not with Osteocyte Lacunar Size
2.2. Bone Phenotype of VDRΔ/Δ Mice Is Similar to That of WT at Baseline but Significantly Different after 2 Months CD
2.3. From the 9-Month-Old VDRΔ/Δ Animals, Only Those on RD Have Similar BMDD Compared to Baseline
3. Discussion
4. Materials and Methods
4.1. Bone Samples
4.2. Quantitative Backscattered Electron Imaging (qBEI)
4.3. Analysis of Structural Morphometric Parameters of Mineralized Bone Tissue
4.4. Osteocyte Lacunae Sections (OLS) Analysis
4.5. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
VDR | vitamin D receptor |
PTH | parathyroid hormone |
PTHrP | parathyroid hormone related protein |
VDRΔ/Δ | mutants with a non-functioning VDR |
sHPT | secondary hyperparathyroidism |
BMDD | bone mineralization density distribution |
qBEI | quantitative backscattered electron imaging |
OLS | osteocyte lacunae sections |
RD | rescue diet |
CD | challenge diet |
ND | normal diet |
Appendix A
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Studied Site | BMDD Parameter | PTH | ALP | Serum Ca |
---|---|---|---|---|
Cortical diaphysis | CaMean | ns | ns | ns |
CaPeak | ns | ns | ns | |
CaWidth | ns | ns | ns | |
CaLow | 0.62 *** | 0.63 *** | −0.69 *** | |
CaHigh | ns | ns | ns | |
Epiphyseal spongiosa | CaMean | −0.72 *** | −0.73 *** | 0.63 *** |
CaPeak | ns | ns | ns | |
CaWidth | ns | ns | ns | |
CaLow | 0.86 *** | 0.81 *** | −0.60 *** | |
CaHigh | ns | ns | ns | |
Metaphyseal spongiosa | CaMean | −0.63 *** | −0.60 *** | ns |
CaPeak | ns | ns | ns | |
CaWidth | ns | ns | ns | |
CaLow | 0.82 *** | 0.77 *** | −0.66 *** | |
CaHigh | ns | ns | ns |
Studied Site | Bone Microstructure | WT BL (n = 5) | VDRΔ/Δ BL (n = 4) | WT 2CD (n = 6) | VDRΔ/Δ 2CD (n = 4) |
---|---|---|---|---|---|
Es | md.BV/TV (%) | 25.5 (19.6; 34.5) | 26.5 (19.2; 27.2) | 32.0 (25.0;35.0) | 9.7 ** (6.3; 19.0) |
md.Tb.Th (μm) | 64 (61; 77) | 69 (60; 78) | 78 (68; 86) | 50 ** (47; 62) | |
md.Tb.N (/mm) | 4.0 (2.9; 4.9) | 3.6 (3.1; 3.9) | 4.0 (3.8; 4.2) | 2.0 * (1.3; 3.0) | |
Ms | md.BV/TV (%) | 11.5 (10.6; 18.3) | 9.6 (9.0; 15.9) | 11.1 (9.8; 13.6) | 7.1 (3.6; 12.0) |
md.Tb.Th (μm) | 41 (37; 45) | 41 (37; 58) | 47 (41; 59) | 38 (28; 54) | |
md.Tb.N (/mm) | 3.0 (2.8; 4.1) | 2.5 (2.2; 2.8) | 2.2 (1.9; 2.6) | 1.7 (1.2; 2.3) |
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Misof, B.M.; Blouin, S.; Hofstaetter, J.G.; Roschger, P.; Zwerina, J.; Erben, R.G. No Role of Osteocytic Osteolysis in the Development and Recovery of the Bone Phenotype Induced by Severe Secondary Hyperparathyroidism in Vitamin D Receptor Deficient Mice. Int. J. Mol. Sci. 2020, 21, 7989. https://doi.org/10.3390/ijms21217989
Misof BM, Blouin S, Hofstaetter JG, Roschger P, Zwerina J, Erben RG. No Role of Osteocytic Osteolysis in the Development and Recovery of the Bone Phenotype Induced by Severe Secondary Hyperparathyroidism in Vitamin D Receptor Deficient Mice. International Journal of Molecular Sciences. 2020; 21(21):7989. https://doi.org/10.3390/ijms21217989
Chicago/Turabian StyleMisof, Barbara M., Stéphane Blouin, Jochen G. Hofstaetter, Paul Roschger, Jochen Zwerina, and Reinhold G. Erben. 2020. "No Role of Osteocytic Osteolysis in the Development and Recovery of the Bone Phenotype Induced by Severe Secondary Hyperparathyroidism in Vitamin D Receptor Deficient Mice" International Journal of Molecular Sciences 21, no. 21: 7989. https://doi.org/10.3390/ijms21217989