MicroRNAs in Vascular Eye Diseases

Since the discovery of the first microRNA (miRNA) decades ago, studies of miRNA biology have expanded in many biomedical research fields, including eye research. The critical roles of miRNAs in normal development and diseases have made miRNAs useful biomarkers or molecular targets for potential therapeutics. In the eye, ocular neovascularization (NV) is a leading cause of blindness in multiple vascular eye diseases. Current anti-angiogenic therapies, such as anti-vascular endothelial growth factor (VEGF) treatment, have their limitations, indicating the need for investigating new targets. Recent studies established the roles of various miRNAs in the regulation of pathological ocular NV, suggesting miRNAs as both biomarkers and therapeutic targets in vascular eye diseases. This review summarizes the biogenesis of miRNAs, and their functions in the normal development and diseases of the eye, with a focus on clinical and experimental retinopathies in both human and animal models. Discovery of novel targets involving miRNAs in vascular eye diseases will provide insights for developing new treatments to counter ocular NV.


Introduction
The Human Genome Project from 1990 to 2003 provided, for the first time, complete comprehensive information on human genome sequences with their entire complexity and thereby started the post-genomic era [1,2]. This era is marked in part by extensive studies on non-coding RNAs (ncRNAs), which do not encode for proteins yet still account for more than 98.5% of human genome transcripts [1,2]. While the housekeeping ncRNAs, such as transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs), have already been shown to exhibit relatively clear functions, the regulatory ncRNAs including long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and circular RNAs (circRNAs), once considered "junk" RNAs, have more recently been found to play important roles in a wide variety of physiological and pathological processes, and hence have become attractive targets in deciphering and treating human diseases. Among these regulatory ncRNAs, miRNAs are arguably one of the most widely studied ncRNAs in biomedical research.
First identified in 1993 [3], miRNAs are a class of naturally occurring small ncRNAs ranging in size from 19 to 25 nucleotides, and their major function is in regulating gene expression at the post-transcriptional level [4]. Since the initial discovery, thousands of miRNAs have been found in various species and the number of miRNAs continues to increase. In the human genome, expression of up to 60% of protein-coding genes may be regulated by miRNAs, indicating their pervasive role in multiple biological processes such as proliferation, apoptosis, differentiation, and development [5][6][7][8]. Importantly, dysregulation of miRNA is found to be involved in many diseases, such as various cancers, cardiovascular diseases, and neurodegenerative disorders. Genetic variation of miRNAs is also linked with several inherited diseases including hearing loss and growth defects. Given their This schematic diagram illustrates the canonical pathway of miRNA biogenesis. The miRNA gene is transcribed by RNA polymerase II (Poly II) to generate the primary miRNA (pri-miRNA) that forms hairpin structures. The long pri-miRNA is then processed by Drosha and DiGeorge syndrome critical region 8 (DGCR8) into the shorter precursor miRNA (pre-miRNA), which is then exported to the cytosol with the help of exportin-5. The pre-miRNA is further Figure 1. MicroRNA (miRNA) biogenesis. This schematic diagram illustrates the canonical pathway of miRNA biogenesis. The miRNA gene is transcribed by RNA polymerase II (Poly II) to generate the primary miRNA (pri-miRNA) that forms hairpin structures. The long pri-miRNA is then processed by Drosha and DiGeorge syndrome critical region 8 (DGCR8) into the shorter precursor miRNA (pre-miRNA), which is then exported to the cytosol with the help of exportin-5. The pre-miRNA is further cleaved by Dicer and transactivation response element RNA-binding protein (TRBP), yielding the miRNA:miRNA* duplex molecule, which is loaded into argonaute (AGO) to unwind and form the functional RNA-induced silencing complex (RISC). The mature miRNA then binds to the seed sequences on the 3 untranslated region (3 UTR) of the target mRNA, leading to its translation repression or cleavage and thereby degradation.

miRNA in the Eye
Just like elsewhere in the body, the function of miRNAs has shown increasing relevance in the eye. In the back of the eye, the retina is comprised of a thin layer of neuronal tissue of diverse cell types and is equipped with a highly specialized light-sensing capacity ( Figure 2). Fine-tuning gene expression for cell differentiation and function is crucial for vertebrate retinal development and proper vision. Given its major impact on gene regulation, miRNA serves a vital role in the retina throughout development and in eye diseases [44][45][46]. Previous animal studies examining the effects of Dicer-deficiency in retinal development identified the differential expression patterns of many miRNAs in vertebrate neural retinas [47][48][49][50][51][52][53][54][55][56], and developed the ability to categorize retinal cells through specific miRNA signatures ( Figure 2   In the developing eye of several species including mammals, amphibians, and fish, miRNA expression patterns were analyzed using a variety of different approaches [48,52,54,[58][59][60][61]. Although the expression profiles of miRNAs vary across species and also differ based on the methods of analysis, some specific miRNAs demonstrate their similar patterns and common roles in retinal development, and in both structural and functional maintenance [62][63][64]. For example, miR-204, one of the most abundant miRNAs in the retina, regulates multiple aspects of eye development [60,61]. Ablation of miR-204 results in abnormal lens formation, altered dorsoventral patterning of the retina, dedifferentiation of the retinal pigment epithelium (RPE), microphthalmia, and coloboma [62,63]. It is strongly expressed in the ganglion cell layer (GCL), the inner nuclear layer (INL), and the RPE [61,65]. In addition, miR-211, another member of the miR-204/211 subfamily, is highly similar to miR-204 in its sequence, target capability, and expression pattern. Loss of miR-211 in mice results in progressive cone dystrophy which is accompanied by the degeneration of cone cells and reduced visual responses detected from electroretinogram (ERG) [66].
Some miRNAs important for brain neural development also play crucial roles in the retina, as a part of the central nervous system (CNS). miR-124, enriched in the vertebrate CNS, is one the most well studied miRNAs in the developing retina [51,52,54,61,64,[67][68][69][70][71][72][73][74]. miR-124 targets Lhx2, a transcriptional factor essential for CNS development, and hence it is important in the maturation and survival of both dentate gyrus neurons and retinal cone photoreceptors. miR-124 is expressed in all retinal neuronal cell layers with the most prominent expression in photoreceptors [46,64]. Abolishing miR-124a in mice leads to mislocalization and apoptosis of cone photoreceptors, altered expression of cone-specific genes and reduced photopic ERG [64]. In addition to photoreceptors, miR-124 is expressed in other retinal neuronal cell layers and contributes to their functions. For instance, in donor eyes from patients with age-related macular degeneration (AMD) and in mouse models of retinal degeneration, miR-124 exhibits a time-dependent altered expression pattern from the outer nuclear layer (ONL) neurons to the Müller glia in the INL, followed by its eventual depletion at a later stage [75]. miR-132, another critical miRNA in brain neural synaptic growth [76], is also important for retinal neurons. As a member of the miR-132/212 cluster, miR-132 shapes brain synapse formation and influences visual cortex plasticity [77]. In the eye, miR-132 promotes axon formation of retinal ganglion cells (RGCs), and is expressed in GCL and INL under the control of brain-derived neurotrophic factor (BDNF) [78].
Given the crucial role of the aforementioned miRNAs in ocular and retinal development and their functional conservation across species, it is plausible that altered expression of miRNAs may lead to ocular disorders, including those vascular eye diseases that are characterized by primary abnormalities in retinal blood vessels which control metabolic availability of oxygen and nutrients to impact retinal neurons.

miRNA and Angiogenesis
To understand the roles of miRNAs in vascular eye diseases, here we first summarize the function of miRNAs in angiogenesis. Angiogenesis is the process of new vessels sprouting from existing vessels, which is orchestrated by various angiogenic stimulators and inhibitors, including miRNAs. Angiogenesis plays crucial roles in both physiological development and homeostasis, as the vascular system delivers nutrients to organs and tissues and removes catabolic products [79,80]. Dysregulation of vascular growth is associated with many cardiovascular diseases, cancers, neurodegenerative disorders, and vascular eye diseases [81,82]. Abnormal angiogenesis disrupts the delivery of oxygen and nutrients, which can lead to an unbalanced metabolic status, and result in structural instability and functional loss of affected tissues. Many eye diseases with vascular components are marked by pathologic ocular neovascularization (NV), characterized by a leaky, fragile, and tuft-liked appearance, which may cause retinal hemorrhage and lead to retinal damage and/or tractional retinal detachment, and ultimately result in vision loss in the most severe cases [83].
The importance of miRNAs in angiogenesis and endothelial function was first established by analysis of Dicer-and Drosha-deficient mice with defective miRNA biosynthesis [84][85][86][87]. Mouse embryos with a Dicer hypomorphic mutation have defective angiogenesis accompanied by reduced miRNA production and dysregulation of angiogenic genes [84,85]. In addition, the knockdown of Dicer and Drosha in human endothelial cells (ECs) suppresses angiogenic functions, including sprouting, tubular formation and migration [86]. Moreover, mice with conditional EC-specific loss of Dicer exhibit reduced vascular endothelial growth factor (VEGF)-driven angiogenesis postnatally, along with an altered set of angiogenic regulating miRNAs [87].

miRNAs Dysregulation in Neovascular Eye Diseases
Increasing evidence indicates that miRNAs and their biogenesis machinery may be altered and dysregulated in neovascular eye diseases, such as diabetic retinopathy (DR), age-related macular degeneration (AMD), and retinopathy of prematurity (ROP), suggesting the potential of using miRNAs as biomarkers and targeting them for potential therapeutics. In this section, we discuss some prominent examples of clinical studies on miRNAs dysregulation in vascular eye diseases, focusing on miRNAs as potential biomarkers (Table 1).

miRNAs in DR
DR is one of the most common microvascular complications of diabetes mellitus (DM), which is now recognized as a global epidemic. In the Western world, DR is a leading cause of vision impairment, particularly among individuals of working-age, and poses a significant economic and life quality burden on patients and society [132][133][134]. To find ways to alleviate this burden, it is important to first understand the biochemical basis underlying DR pathogenesis. Hyperglycemia induces alteration in cellular metabolism and causes oxidative injury. Prolonged exposure to hyperglycemia and metabolic changes leads to microvasculature damage in the retinas of diabetic patients. Progressive retinal ischemia eventually stimulates the expression of hypoxia-induced growth factors, such as VEGF, that promote retinal NV [135]. Retinal NV can then cause vitreous hemorrhage or tractional retinal detachment to result in severe vision loss [135,136]. Other major events involved in DR pathogenesis are the breakdown of the blood-retinal barrier and the consequent vascular leakage and thickening of the retina [137]. DR develops in approximately 50% patients with Type 1 diabetes mellitus (T1DM) and over 40% of patients with Type 2 diabetes mellitus (T2DM) by the first decade of incidence [138].
In addition to diagnosing DR through histological examination of the fundus vasculature, circulating miRNAs were found to play critical roles in DR development, suggesting that miRNAs could also serve as new biomarkers in detecting or predicting the progress of retinopathy and furthermore, the overall progress of DM [139][140][141]. Analysis of circulating miRNAs from serum or plasma samples of DM patients both with and without DR showed altered expression levels of many miRNAs throughout different patient populations (e.g., ages, type of DM, years after onset, etc.,) [142]. Among them, miR-27b and miR-320a are the two miRNAs mostly associated with the risks of DR in T1DM [117]. miR-221, an anti-angiogenic miRNA, is significantly altered in DM and is involved in the DM physiopathology and macrovascular complications associated with T2DM [113][114][115][116]. In addition, other circulating miRNAs including miR-126 [105][106][107], miR-150 [109], miR-155 [110], and miR-200b [111] are also dysregulated in DR patients, as well as in pre-clinical animal models of DR [143][144][145][146][147][148][149][150][151][152]. These findings indicate the complex regulation of miRNAs in DR and the potential of miRNAs as biomarkers and/or therapeutic targets for treating DR.

miRNAs in Neovascular Age-Related Macular Degeneration (wet AMD)
AMD is a leading cause of irreversible loss of central vision in the elderly. Approximately 10-18% of individuals between 65 and 75 will lose some central vision as a result of AMD, while this number increases to 30% for those aged 75 or older [153]. There are two major clinical types of AMD: Atrophic (dry form) AMD with photoreceptor and RPE atrophy; and neovascular (wet from) AMD which is characterized by pathologic subretinal vessels originating from the choroid, i.e., choroidal neovascularization (CNV), the hallmark of wet AMD. Although only 10-20% of AMD patients develop wet AMD, this form of the disease accounts for approximately 80% of severe visual loss in AMD cases [154]. Central vision loss occurs when pathological choroidal neovessels protrude into the subretinal space and subsequently leak blood and cause exudates and hemorrhagic detachment of the retina, thereby resulting in irreversible photoreceptor damage [155].
As a complex, multifactorial, and progressive disease, AMD is linked with both genetic (including complement) and environmental risk factors [155]. Certain miRNAs associated with the complement factor H (CFH)-mediated inflammatory degeneration and neovascularization are dysregulated in the circulating blood or ocular tissues isolated from AMD patients [118,121,156,157]. Two studies identified differential sets of miRNAs altered in plasma collected from wet AMD patients compared to healthy subjects [118,156]. In one study, 16 miRNAs were found to be dysregulated out of 384 miRNAs screened in wet AMD patient plasma samples using quantitative real-time PCR-based methods, with 10 of the 384 miRNAs being exclusively expressed in the wet AMD patient group [118]. Another similar study using next-generation sequencing identified that 3 out of 203 circulating miRNAs were significantly altered in plasma from wet AMD patients vs. non-AMD controls [156]. Moreover, miRNA microarray screening found 23 out of 337 miRNAs were upregulated in the serum from both dry and wet AMD patients vs. non-AMD cohorts. Among them, only 3 miRNAs were expressed at significantly higher levels in the serum of patients with wet AMD [119]. The difference in miRNA profiles from these studies may reflect the variation in their miRNA screening methodology, the nature of samples (plasma vs. serum), the diverse patient population and the different inclusion criteria.

miRNAs in Retinopathy of Prematurity (ROP)
ROP is an ocular disease associated with abnormal retinal vascular development that occurs in premature infants and contributes to 6-18% of blindness in children in the developed countries [158][159][160].
ROP is a two-phase disease, beginning with incomplete retinal vessel growth after premature birth, which results in a peripheral avascular zone. As the infant matures, increasing metabolic activities of the peripheral avascular retina cause tissue ischemia and hypoxia. This stimulates a second phase of hypoxia-driven pathological vessel proliferation. In severe cases, pathologic neovessels in the second phase can cause tractional retinal detachment, ultimately leading to blindness [161][162][163]. Current ablation treatments may substantially reduce the incidence of blindness by 25% and improve long-term outcomes in infants with severe ROP. However, these treatments do not address the underlying causes of ROP or other comorbidities, including the failure of normal neuronal and vascular development [163][164][165]. Whether miRNA dysregulation contributes to ROP development has been a subject of recent studies assessing the diagnostic and therapeutic potential miRNAs as novel ROP biomarkers.
Plasma miRNAs were evaluated in premature infants with ROP and compared to preterm infants without ROP in a recent study using high-throughput quantitative real-time PCR [127]. Four out of 46 plasma miRNAs were significantly altered in ROP patients, with miR-23a and miR-200b-3p being upregulated and miR-27b-3p and miR-214-3p being downregulated [127]. miR-23a represses anti-angiogenic genes, such as sprouty2 (Spry2) and semaphorin6A (Sema6A), and Sema6D [97], and hence might be pro-angiogenic in ROP pathogenesis. Expression of miR-200b correlates with VEGF expression [128], and is an angiogenic regulator targeting Ets-1 in ECs [112]. On the other hand, miR-27b and miR-214 are anti-angiogenic factors as they inhibit VEGF family protein expression [129][130][131]. Dysregulation of these miRNAs in ROP is consistent with their potential roles in mediating pathological angiogenesis in ROP development.
Profiles of miRNAs are evaluated in several pre-clinical animal models of ROP. The miRNA expression patterns in different models vary widely, and may depend on a number of factors including animal species (mice vs. rats), the oxygen condition, time point of tissue collection, and analysis methods. Some miRNAs exhibit dramatically varied expression patterns in different models, including miR-126 [144,166], miR-145 [167,168], miR-150 [72,167,169], and miR-155 [170]. The function of these miRNAs as ocular angiogenic regulators are discussed in detail in the next section.

Dysregulated miRNAs in Experimental Models of Pathological Ocular Angiogenesis
Expression patterns of miRNAs were investigated in several animal models of ocular NV that mimic pathological features of human vascular eye diseases. Of particular relevance to this review is the oxygen-induced retinopathy (OIR) model, mimicking pathological retinal NV in ROP and DR, and the laser-induced CNV model, mimicking wet AMD. By exposing the newborn experimental animals (rodents, in most cases) to continuous hyperoxic or cycling oxygen conditions, the OIR model reliably reproduces the phenotypes of ROP-characterized by an initial phase of vaso-obliteration and a subsequent phase of hypoxia-induced NV [171,172]. As the current diabetic models fail to consistently develop proliferative retinopathy in rodents, the OIR model also serves as a platform to facilitate the investigation of the ischemic angiogenesis aspect of DR [57]. For wet AMD, the rodent model of laser-induced CNV is the most standard and widely used animal model for investigating many aspects of choroidal angiogenesis. In this model an argon laser is used to induce rupture of the Bruch's membrane, which increases pro-angiogenic and inflammatory factors and stimulates new choroidal vessels growth into the laser-injured subretinal areas to form CNV [173][174][175]. The pioneering work in the laser-induced CNV and the OIR models has laid the experimental foundation for establishing the therapeutic value of anti-VEGF therapies as these are useful models for investigating the mechanisms of NV and evaluating novel anti-neovascular therapies [176,177], including the role of miRNAs. Some examples of well-characterized miRNAs that regulate pathological ocular angiogenesis in experimental models are reviewed here.

miR-126
miR-126 is one of the angiomiRs implicated in the regulation of angiogenic factors including VEGF and FGF for vascular growth, and regulates embryonic angiogenesis and cardiac angiogenesis [91,92]. miR-126 exhibits significant downregulation in the choroids of mice with laser-induced CNV [123], as well as in rodent OIR retinas and choroids [144,166]. Moreover, miR-126 knockout mice have vascular lesions in the peripheral areas of choroids in mature adults, delayed choroidal vascular development, and focal choroidal vascular atrophy in aged mice [178]. In OIR mice, miR-126 supplementation inhibits retinal neovascularization and blood-retinal barrier breakdown [144,179]. Overall these findings indicate that miR-126 is required for maintaining ocular vasculature integrity in pathological conditions.

miR-132
In the mouse models, miR-132 plays a crucial role in promoting angiogenesis by targeting Rasa1 (encoding p120RasGAP) [180][181][182][183]. Inhibition of miR-132 in the mouse models of OIR and retinal angiomatous proliferation (RAP) promotes EC quiescence and prevents NV by enhancing the expression of p120RasGAP [181]. Inhibition of miR-132 reduces EC function and suppresses growth factor-mediated developmental retinal and tumor angiogenesis in vivo and in vitro [182]. Furthermore, silencing miR-132 also suppresses corneal angiogenesis after eye infection with herpes simplex virus [184]. Beyond vascular endothelium, miR-132 is also expressed in the eye by RGCs and is up-regulated in response to BDNF [78]. These findings suggest the angiogenic functions of miR-132 in neovascular ocular diseases and its additional function in retinal neuronal health.

miR-145
miR-145 is co-transcribed with miR-143 as a cluster, which is generally considered as a tumor suppressor cluster in cancer cells [185]. However, in the mouse model of lung adenocarcinoma, tumor-specific deletion of miR-143/145 resulted in diminished angiogenesis; whereas overexpression of miR-143/145 stimulated EC proliferation in the tumor mass [186], indicating a surprising pro-tumor and pro-angiogenic function of miR-143/145, likely reflecting its diverse role in a context-dependent manner. Mice with systemic knockout of miR-143/145 are viable and show no overt abnormalities in cardiac structure and vascular smooth muscle cell differentiation [187]. In the OIR model, miR-145 is significantly upregulated in the retinas at P17 when compared to the age-matched normoxic control mice [167]. Intravitreal injection of miR-145 inhibitors suppresses NV in OIR. Moreover, modulation of miR-145 in vitro alters human retinal microvascular endothelial cell (HRMEC) angiogenic functions by targeting tropomodulin 3 (Tmod3), an actin-capping protein. miR-145 may thereby influence angiogenesis in ocular neovascular diseases through the modulation of the cytoskeletal architecture dynamics, and EC morphological changes [168] (Figure 3). Other studies also showed that miR-145 in retinal ECs may attenuate oxidative stress and inflammation induced by high-glucose, further supporting its role in DR [188]. Within the eye, miR-143/145 cluster also regulates intraocular pressure through the regulation of actin dynamics and trabecular meshwork contractility [189]. miR-145 also promotes ganglion cell survival in DR [190]. These findings demonstrate multiple roles of miR-143/145 in various ocular cell types and eye diseases. functions by targeting tropomodulin 3 (Tmod3), an actin-capping protein. miR-145 may thereby influence angiogenesis in ocular neovascular diseases through the modulation of the cytoskeletal architecture dynamics, and EC morphological changes [168] (Figure 3). Other studies also showed that miR-145 in retinal ECs may attenuate oxidative stress and inflammation induced by highglucose, further supporting its role in DR [188]. Within the eye, miR-143/145 cluster also regulates intraocular pressure through the regulation of actin dynamics and trabecular meshwork contractility [189]. miR-145 also promotes ganglion cell survival in DR [190]. These findings demonstrate multiple roles of miR-143/145 in various ocular cell types and eye diseases.

miR-146a
mR-146a has been linked to the innate immune response, inflammation, and age-related neurodegenerative disorders [191]. Samples from patients with wet AMD and mouse retinas with

miR-146a
mR-146a has been linked to the innate immune response, inflammation, and age-related neurodegenerative disorders [191]. Samples from patients with wet AMD and mouse retinas with selective glial cell ablation showed an upregulation of miR-146a and an involvement in CFH-mediated inflammation [124,125]. In the pre-clinical models of DR, miR-146a is upregulated in the retinal ECs with transactivation by nuclear factor-kappaB (NF-κB). Upregulation of miR-146a exerts negative regulation in multiple pathways of NF-κB activation, which suggests its correlation to inflammatory responses in DR [148,[192][193][194][195][196][197]. Specifically, miR-146a upregulates inflammatory cytokines in the diabetic retina and kidney [197], protects HRMECs [194], reduces retinal microvascular leakage, and improves visual function in diabetic rats [198]. Moreover, diabetes induces rhythmic dysregulation of miR-146a and its inflammatory genes in human retinal endothelial cells [195]. These findings all point to the potential implication of miR-146a in DR development.

miR-150
miR-150 is a well-studied miRNA which was initially identified by its regulatory effects in lymphocyte development and differentiation [199][200][201]. Monocytic-secreted miR-150 influences angiogenesis in cancer and diabetes by modulating target gene expression in recipient ECs [202,203]. In the retina, miR-150 is enriched in retinal ECs more than in any other nuclear layers [169]. In OIR, miR-150 is substantially reduced in OIR mouse retinas with specific downregulation in OIR neovessels, and regulates expression of several angiogenic factors, such as Cxcr4 (C-X-C chemokine receptor type 4), Dll4 (Delta like ligand 4) and Fzd4 (Frizzled-4) [72,169] (Figure 3). Treatment of miR-150 in vivo, via intraocular injection into the OIR mice, or in vitro, by transfection into HRMECs, demonstrated that miR-150 reduces pathological NV and regulates EC angiogenic functions in a VEGF-independent manner by targeting CXCR4, DLL4, and FZD4 [169]. Furthermore, miR-150 knockout mice show increased size of laser-induced CNV lesion [169], suggesting the role of miR-150 as an intrinsic inhibitor of pathological ocular angiogenesis. Similarly, miR-150 deletion leads to increased pulmonary angiogenesis in a hyperoxia-induced lung injury model [204], and exacerbates high fat diet-induced retinal NV in diabetic mice [149], suggesting an overall protective role of miR-150 against pathological angiogenesis. 7.6. miR-155 miR-155 is significantly upregulated in retinas of several ocular disease models, including the mouse OIR model [167,170], the rat models of light-induced retinal degeneration [205] and streptozotocin (STZ) -induced diabetes [148], as well as in human patients with AMD [124]. miR-155 is a HIF-dependent miRNA and its deficiency results in the reduction of the avascular area and NV in the mouse OIR model [150,170]. By targeting CCN1-a cysteine-rich and integrin-binding matricellular protein, upregulated miR-155 disturbs the normal retinal vessel growth in mice [170]. In addition to regulation of angiogenesis, miR-155 is also involved in inflammatory and immunomodulatory signaling pathways, which are of crucial importance in pathological angiogenesis [206]. In the preclinical model of STZ-induced DR, miR-155 was identified as a NF-κB-and VEGF-responsive miRNA [148]. miR-155 has also been shown to regulate CFH in AMD [124], further supporting its role in ocular angiogenesis and inflammation in eye diseases.

miR-21
miR-21 plays an important role in the regulation of angiogenesis, tumor growth and metastasis, as well as in cardiac hypertrophy [126,148,207,208]. This miRNA is downregulated in the plasma of wet AMD patients [118]. As such, miR-21 may play an important role in AMD pathogenesis for its involvement in the regulation of vascular growth, as exhibited by its high expression in retinal ECs [126]. This notion is supported by the fact that overexpression of miR-21 reduces CNV lesions in the laser-induced CNV mice. In addition, stimulated expression of miR-21 inhibits cultured EC proliferation and migration by targeted inhibition of RhoB, which controls the dynamics of actin-filament and thereby affects the EC function [126]. However, in the rat diabetic model, miR-21, as well as miR-146 and miR-155, are upregulated in the retinas and retinal ECs along with NF-κB and/or VEGF activation [148]. In the retinas of leptin receptor-deficient (db/db) mice, miR-21 was also significantly upregulated while its target gene PPARα (peroxisome proliferator-activated receptor-α), was downregulated [209]. These different findings from several animal models may reflect an underlying difference among various eye disease models or assay conditions, yet together indicate a potential disease-modifying effect of miR-21.

Conclusions
In summary, miRNAs are potent effectors in the post-transcriptional regulation of gene activity and play an important role in the modulation of retinal homeostasis and diseases including vascular eye diseases. Although the miRNA expression profiles from different experimental models of ocular angiogenesis differ in a disease-and model-dependent manner, these studies provide valuable clues to understanding the functions of dysregulated miRNAs in retinopathies. Furthermore, dysregulation of specific miRNAs can be utilized to identify potential miRNA candidates for therapeutic intervention. With expanding knowledge of miRNA profiles and their molecular mechanisms in eye development and ocular diseases, miRNAs can be harnessed for their capacities as biomarkers and their potential to be targeted for treating neovascular ocular diseases. In fact, the emerging miRNA therapeutics with its ability to target multiple pathological target genes may likely yield one of the most exciting breakthroughs in the current treatment options for ocular diseases. With the current surge in omics research providing vast amounts of datasets, identification of critical miRNA targets for drug development presents considerable potential for generating such novel therapies for vascular eye diseases.