Monoterpenes and Their Derivatives—Recent Development in Biological and Medical Applications

Monoterpenes, comprising hydrocarbons, are the largest class of plant secondary metabolites and are commonly found in essential oils. Monoterpenes and their derivatives are key ingredients in the design and production of new biologically active compounds. This review focuses on selected aliphatic, monocyclic, and bicyclic monoterpenes like geraniol, thymol, myrtenal, pinene, camphor, borneol, and their modified structures. The compounds in question play a pivotal role in biological and medical applications. The review also discusses anti-inflammatory, antimicrobial, anticonvulsant, analgesic, antiviral, anticancer, antituberculosis, and antioxidant biological activities exhibited by monoterpenes and their derivatives. Particular attention is paid to the link between biological activity and the effect of structural modification of monoterpenes and monoterpenoids, as well as the introduction of various functionalized moieties into the molecules in question.


Introduction
Plants have been used for medical purposes for centuries. As such, they have been studied scientifically and traded commercially. Monoterpenes belong to a large and diverse group of naturally occurring compounds. The basic structure of monoterpenes, or monoterpenoids, consists of two linked isoprene units; some of the representative compounds are given in Figure 1. They might be cyclized and oxidized in a variety of ways. Due to the low molecular weight, many of them exist in the form of essential oils. The numbers of monoterpenes have antimicrobial, anti-inflammatory, antioxidant, antipruritic, hypotensive, and analgesic pharmacological properties.
Functionalization of natural compounds exhibiting native biological activity is one of the most efficient approaches for the synthesis of biologically active substances in medicinal chemistry. This explains, inter alia, why the studies of terpenes and their derivatives are interesting and relevant. Consequently, there is growing body of literature regarding new modifications to these fascinating natural compounds and the corresponding biological and medical applications. Despite the importance of this class of compounds, there are limited reviews on the biological and pharmacological properties of monoterpene derivatives [1][2][3][4][5][6]. Hence, we considered it appropriate to provide a review of selected monoterpene derivatives and their diverse therapeutic activities. We pay particular attention to the structural changes in monoterpenes and the introduction of special groups that increase the biological activity of the studied derivatives. We focus on structural modifications of geraniol (representative of acyclic monoterpenes), thymol (representative of monoterpene monocycles), myrtenal, pinene, camphor, and borneol (representatives of monoterpene bicycles); see Figure 2 for an overview. We present those Functionalization of natural compounds exhibiting native biological activity is one of the most efficient approaches for the synthesis of biologically active substances in medicinal chemistry. This explains, inter alia, why the studies of terpenes and their derivatives are interesting and relevant. Consequently, there is growing body of literature regarding new modifications to these fascinating natural compounds and the corresponding biological and medical applications. Despite the importance of this class of compounds, there are limited reviews on the biological and pharmacological properties of monoterpene derivatives [1][2][3][4][5][6]. Hence, we considered it appropriate to provide a review of selected monoterpene derivatives and their diverse therapeutic activities. We pay particular attention to the structural changes in monoterpenes and the introduction of special groups that increase the biological activity of the studied derivatives. We focus on structural modifications of geraniol (representative of acyclic monoterpenes), thymol (representative of monoterpene monocycles), myrtenal, pinene, camphor, and borneol (representatives of monoterpene bicycles); see Figure 2 for an overview. We present those monoterpene derivatives that exhibit several important biological properties; they are often multi-functional or exhibit interesting biological selectivity in their activity.

Geraniol
Geraniol, trans-3,7-dimethyl-2,6-oktadien-1-ol, is a primary, acyclic, doubly unsaturated terpene alcohol with a characteristic flowery, rose-like odor. This monoterpene is extracted from the essential  Chavez et al. [11] showed interesting results regarding biologically active geraniol derivatives. A large series of geranylated phenol/methoxyphenol compounds were synthesized to test them in vitro as inhibitor agents of mycelial growth of Phytophthora cinnamomi. It turned out that geranylated derivatives containing two hydroxyl groups attached to the aromatic ring, or one of them exchanged by a methoxy group, are the most efficient inhibitors. Some selected structures from the set studied by Chavez et al. are shown in Figure 4 (compounds 3-8). Importantly, the activity of those derivatives is as effective as that of Metalaxil ® , a commonly known commercial fungicide, used as an inhibitor of mycelial growth of P. cinnamomi. Some of the discussed compounds are also active against Botrytis cinerea, which makes them possibly useful for biotechnological purposes since they may control the infection with more than one pathogen. Chavez et al. [11] showed interesting results regarding biologically active geraniol derivatives. A large series of geranylated phenol/methoxyphenol compounds were synthesized to test them in vitro as inhibitor agents of mycelial growth of Phytophthora cinnamomi. It turned out that geranylated derivatives containing two hydroxyl groups attached to the aromatic ring, or one of them exchanged by a methoxy group, are the most efficient inhibitors. Some selected structures from the set studied by Chavez et al. are shown in Figure 4 (compounds 3-8). Importantly, the activity of those derivatives is as effective as that of Metalaxil ® , a commonly known commercial fungicide, used as an inhibitor of mycelial growth of P. cinnamomi. Some of the discussed compounds are also active against Botrytis cinerea, which makes them possibly useful for biotechnological purposes since they may control the infection with more than one pathogen. Biofouling that occurs on the surface of submerged objects, which causes technical and economic problems mainly in the marine industry, but also in many biotechnological processes, is a stimulus to the design and synthesis of effective, safe, and at the same time environmentally benign antifouling compounds. Motivated by these challenges Takamura's research group designed and synthesized a hybrid of molecules containing geraniol and butanolide moieties [12]. A set of prepared geraniol derivatives consists of eight various molecules 13, some representatives of this series (14)(15)(16)(17) are included in Figure 6. Biological evaluation of presented geraniol-butanolide hybrid compounds 13 proved that those molecules exhibit antifouling activity against the cypris larvae of the barnacle Balanus amphitrite (Darwin, 1854), having the values of EC50 within the range of 0.30-1.31 µM. Since most of those compounds, 13 show low or no toxicity geraniol derivatives might be successfully applied as antifouling agents. Biofouling that occurs on the surface of submerged objects, which causes technical and economic problems mainly in the marine industry, but also in many biotechnological processes, is a stimulus to the design and synthesis of effective, safe, and at the same time environmentally benign antifouling compounds. Motivated by these challenges Takamura's research group designed and synthesized a hybrid of molecules containing geraniol and butanolide moieties [12]. A set of prepared geraniol derivatives consists of eight various molecules 13, some representatives of this series (14)(15)(16)(17) are included in Figure 6. Biological evaluation of presented geraniol-butanolide hybrid compounds 13 proved that those molecules exhibit antifouling activity against the cypris larvae of the barnacle Balanus amphitrite (Darwin, 1854), having the values of EC 50 within the range of 0.30-1.31 µM. Since most of those compounds, 13 show low or no toxicity geraniol derivatives might be successfully applied as antifouling agents. derivatives consists of eight various molecules 13, some representatives of this series (14)(15)(16)(17) are included in Figure 6. Biological evaluation of presented geraniol-butanolide hybrid compounds 13 proved that those molecules exhibit antifouling activity against the cypris larvae of the barnacle Balanus amphitrite (Darwin, 1854), having the values of EC50 within the range of 0.30-1.31 µM. Since most of those compounds, 13 show low or no toxicity geraniol derivatives might be successfully applied as antifouling agents.
The derivatives with the thymol-based scaffolds have gained much attention owing to their antibacterial, antifungal, anti-inflammatory, anticancer, antitumor, anti-HIV, antiviral, antipyretic, anticonvulsant, and antidepressant properties.
Other research teams synthesized metronidazole ester 30 and ether 31 with thymol and investigated their in vitro antibacterial activity on Helicobacter pylori (the ATCC 26695 and P12) and Clostridium (C. perfringens) strains. Both compounds demonstrated good activity against H. pylori strain. It is worth noting that the ether derivative (31) is more active than the ester (Figure 8) [34].
Wang et al. designed and synthesized thymol esters with different heterocyclic units 41 ( Figure 10) and tested them for their in vitro antifungal activity against five plant pathogenic fungi (Alternaria solani, Botrytis cinerea, Fusarium oxysporum, Pyricularia oryzae, and Rhizoctonia solani) by the mycelium growth rate method. Some synthetic esters show good to excellent antifungal activity, especially with furan, thiophene, and pyridine unit. The presence of a bromine atom on the para position of benzene moiety increased the antifungal activity [39].
The antioxidant activity of thymol is attributed to its phenolic structure and its redox properties, which play an essential role in the adsorbing and neutralizing of free radicals or decomposing peroxides. A wide range of thymol derivatives was explored for structure-radical scavenging activity. By comparing the structural properties of thymol derivatives, their antioxidant activity was explained by the formation of an intramolecular hydrogen bond. Three thymol derivatives, 8,10-dihydroxy-9-isobutyryloxythymol 42, 8-methoxy-9-hydroxythymol 43, and 8,9-dehydro-10-hydroxythymol 44, were isolated from Zataria multiflora extract. The antioxidant activity of these compounds was evaluated with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Compound 44 has higher antioxidant potential with IC 50 value of 62.5 µM and BDE = 79.2 kcal/mol. These results show that the presence of an unsaturated double bond is the main factor that determines the antioxidant activity of the investigated compounds ( Figure 11) [40]. that the presence of an unsaturated double bond is the main factor that determines the antioxidant activity of the investigated compounds ( Figure 11) [40].  Nagle et al. [30] evaluated 1,2-dihydro-6-(4-hydroxy-5-isopropyl-2-methylphenyl)-2-oxo-4-arylpyridine-3-carbonitriles for their in vitro antioxidant and antimicrobial activity. Compounds 45 shows much better antioxidant activity than other derivatives ( Figure 11).
Recently, Ashraf's group has studied the substituted benzoic acid and cinnamic acid thymol moieties.
Bendre and co-worker synthesized a series of thymol-based benzamides 47 ( Figure 11), structurally similar to paracetamol [42]. These compounds were subjected to antioxidant testing by DPPH assay. The results revealed that all the synthesized compounds exhibit notable antioxidant activity and also molecular docking studies indicated that they are good inhibitors of heme oxygenase-1.
A study conducted by Chen and co-workers evaluated the cytotoxic activity of various thymol derivatives isolated from the root of Eupatorium cannabinum subsp. Asiaticum Kitam. All compounds Rajput et al. [44] synthesized thymol-based hydrazone derivatives 50-52 by the condensation of thymol with substituted 2-acetohydrazide of carvacrol and thymol. All compounds were screened for their antioxidant activity with DPPH assay and anticancer activity with SRB assay for the pancreas and colon. The molecular docking studies of all the synthesized derivatives were performed on the COX-2 protein enzyme. In the anticancer and antioxidant tests, thymol hydrazones indicate moderate activities. The molecular docking studies demonstrated excellent binding affinity towards the enzyme ( Figure 12). New thymol derivatives 53 and 54 ( Figure 12) were isolated from the roots of Eupatorium chinense L. and tested in vitro for their cytotoxic activities against three cancer cell lines CNE 2, Caski, and HGC-27. The considerable cytotoxic effects against the three cancer cell lines of compound 53 were observed with the IC50 values ranging from 4.2 to 11.9 µM [45].
More recently, Shi and co-workers isolated a few thymol derivatives from the overground parts Rajput et al. [44] synthesized thymol-based hydrazone derivatives 50-52 by the condensation of thymol with substituted 2-acetohydrazide of carvacrol and thymol. All compounds were screened for their antioxidant activity with DPPH assay and anticancer activity with SRB assay for the pancreas and colon. The molecular docking studies of all the synthesized derivatives were performed on the COX-2 protein enzyme. In the anticancer and antioxidant tests, thymol hydrazones indicate moderate activities. The molecular docking studies demonstrated excellent binding affinity towards the enzyme ( Figure 12).
New thymol derivatives 53 and 54 ( Figure 12) were isolated from the roots of Eupatorium chinense L. and tested in vitro for their cytotoxic activities against three cancer cell lines CNE 2, Caski, and HGC-27. The considerable cytotoxic effects against the three cancer cell lines of compound 53 were observed with the IC 50 values ranging from 4.2 to 11.9 µM [45].
More recently, Shi and co-workers isolated a few thymol derivatives from the overground parts of Eupatorium fortunei Turcz. All compounds were screened for their cytotoxic activities against four human cancer cell lines using MTT assay. Compounds 9-O-angeloyloxy-8,9-dehydrothymol 55, 9-(3-methyl-2-butenoyloxy)-8,10-dehydrothymol 56 and 9-O-angeloyl-8,10-dehydrothymol 57 ( Figure 12) exhibit notable cytotoxicities with IC 50 values 6.24-11.96 µM against MCF-7, HeLa, A549, and Hep G-2 cell lines. The preliminary research indicates that the presence of the methyl group at the C-1 position and the substituted group at C-9 in the structure of these thymol derivatives seem to be essential for the inhibition of the proliferation of four cancer cell lines [46].
The aromatic esters of thymol 63 ( Figure 14) were obtained, and their inhibition of the enzyme acetylcholinesterase, and larvicidal activity against Aedes aegypti was evaluated. The presence of a benzoate group containing electron-withdrawing substituents on the aromatic ring resulted in the inactivation of the compound against the mosquito larvae, while thymol 4-methoxybenzoate 63e with electron-donating group exhibits much better activity [50].
Another group of researchers synthesized novel oxypropanolamines containing thymol structure 65 and 66 and evaluated their inhibitory effects on α-glycosidase, cytosolic carbonic anhydrase I and II isoforms (hCA I and II), and acetylcholinesterase enzymes (AChE) ( Figure 14). The results demonstrated that all compounds effectively inhibit α-glycosidase, hCA I and II, and AChE enzymes (with Ki values in the range of 63.85-851.05 µM for α-glycosidase, 1.11-17.34 µM and 2.97-17.83 µM for hCA I and hCA II, respectively, and 13.58-31.45 µM for AChE). Furthermore, the authors concluded that derivatives 66a-c exhibited the most significant antibacterial activity on A. baumannii, P. aeruginosa, E. coli, and S. aureus strains among all the tested compounds [52].
Henry and co-workers synthesized seven new alkyl 4-oxobutanoate derivatives of thymol 67 and screened them for tyrosinase inhibitory activity using mushroom tyrosinase ( Figure 14). Generally, the alkyl 4-oxobutanoate derivatives exhibit better inhibitory effects than the ethyl thymol 4-oxobutanoic acids. The data demonstrated the correlations between the structure of the alkyl side chain and tyrosinase inhibitory activity. The best results were obtained for esters containing three or four carbon atoms in the alkyl chain with IC 50 (Figure 15). The authors also investigated the antiviral activity of Anaphalis triplinervis (Sims) Sims ex C.B.Clarke EO and its major compound THQ against ZIKV. Virological assays were performed on human lung epithelial A549 cells infected with either GFP reporter ZIKV or epidemic viral strain. To evaluate the acute toxicity of THQ in vivo the zebrafish assay was used. The results demonstrated that both EO and THQ inhibit ZIKV infection in human cells with IC 50 values of 38 and 45 µM. It was noted that the inoculation of THQ at the antiviral effective concentration does not lead to any stress and does not impact fish survival [54].
Many studies have demonstrated that serotonin receptors, especially the 5-HT6 subtype, indicate great suitability for the potential therapy of the topic civilization CNS diseases.
Latacz et al. performed an exploration into the group of 1,3,5-triazine-methylpiperazines and screened them for pro-cognitive action and anxiolytic-like effect in the behavioral in vivo and in vitro tests. The best results were observed for the thymol triazine derivative. Compound 4-((2-isopropyl-5-methylphenoxy)methyl)-6-(4-methylpiperazin-1-yl)-1,3,5-triazin-2-amine 69 ( Figure 15) shows the most promising pharmacological and drug-likeness profile and seems to be of higher importance due to its structure lacking the indole and sulfone fragments and the best 5-HT6R binding properties [55].
Adamantane derivatives have been widely used in clinical practice [66]. The applicability of compounds with both the adamantane and myrtenal moieties was investigated in various biological tests.
Suslov and co-workers synthesized a few new nitrogen-containing compounds with adamantane and myrtenal framework and studied the anxiolytic activity of the obtained products in male Balb/C mice in the elevated plus-maze (EPM) test. The results of this investigation showed that N-{[(1R,5S)-6,6-dimethylbicyclo[3.1.1]hept-2-en-2-yl]-methyl}adamantan-2-amine 72 exhibits tranquilizing activity and pronounces anxiolytic activity in the EPM test ( Figure 16) [67]. Compound 72 was tested in vitro for antiviral activity against the influenza virus A/California/07/09 (H1N1)pdm09. The results indicated that the introduction of a myrtenal fragment led to an increase in the antiviral activity of adamantylamine derivatives against the adamantylamine resistant virus: The selectivity index of the majority of the synthesized amines was higher than that of rimantadine and amantadine [68]. Compound 73 was synthesized from 1-aminoadamantane and (-)-myrtenal and its cytotoxic activity against human cancer cells CEM-13, MT-4, and U-937 was studied. Adamantylamine 73 revealed a high activity against all tumor lines used (CTD 50 = 12-21 µM) along with low toxicity for MDCK cells (CTD 50 = 1500 µM). Furthermore, no genotoxic effect on normal cells was observed [69], whereas myrtenal-derived amine 74 synthesized from 1-aminoadamantane and (+)-myrtenal inhibited anti-Tdp1 activity with IC 50 values of 6.1µM ( Figure 16) [70].
Adamantane derivatives containing N atoms at the bridging positions manifested analgesic activity in vivo tests. Suslov's group prepared a series of new compounds linking diazaadamantane and monoterpene moieties such as substituted 5,7-dimethyl-1,3-diazaadamantan-6-ones, which were vastly potent analogs of analgesic. The results showed that analgesic activity was completely lost if the 5-and 7-alkyl substituents were lengthened by one or two CH 2 groups in 5,7-dialkyl-1,3-diazaadamantan-6-ones substituted with monoterpenoids in the aminal position 75. In addition, compound 76a with a 6-OH instead of the carbonyl group was found to be active in the visceral pain test and it does not show analgesic activity for tactile pain, while the reduction in the ketone to methylene group in diazaadamantane 76b with (-)-myrtenal in the aminal position of the heteroadamantane structure affected the loss of analgesic properties in both tests ( Figure 16) [71].
Song and co-workers focused their attention on the preparation of novel 3-cyanopyridine derivatives of (-)-β-pinene 83 ( Figure 19) [77]. All compounds were evaluated in vitro against four bacteria (Klebsiella pneumoniae, Enterobacter aerogenes, Staphylococcus aureus, Staphylococcus epidermidis) and a fungus (Candida albicans). The tested compounds exhibited moderate antibacterial activity against the Gram-positive bacteria S. aureus, S. epidermidis, weak antibacterial activity against the Gram-negative bacteria K. pneumoniae, E. aerogenes, and moderate antifungal activity against C. albicans.
Thio derivatives of pinane series exhibit high antioxidant, antifungal, antiaggregation, anticoagulant, and pronounced antimycotic activity. Nikitina et al. [79]   Ye et al. [80] designed and synthesized novel α-pinene derivatives as a potential antitumor agent. It was found that compound 86 (6,6-dimethylbicyclo  Ye et al. [80] designed and synthesized novel α-pinene derivatives as a potential antitumor agent. It was found that compound 86 (6,6-dimethylbicyclo  Ye et al. [80] designed and synthesized novel α-pinene derivatives as a potential antitumor agent. It was found that compound 86 (6,6-dimethylbicyclo[3.1.1]hept-2-en-2-yl)methyl-4methylbenzenesulfonate) (Figure 20) exhibits the strongest inhibition on hepatoma carcinoma cell BEL-7402. The results showed that 86 demonstrates good antiliver cancer activity with the IC50 of 84.7 µM in vitro, and inhibits tumor growth in vivo with dose-dependent. The authors suggested that compounds 86, apart from arresting the growth of hepatoma cells in the S phase, induce apoptosis in hepatoma cells, down-regulate the expression of C-myc, CDK2 and CyclinE, and up-regulate the level of p53.  A series of new chiral N-terpenyl benzisoselenazol-3(2H)-ones with three monoterpene moietiesp-menthane, pinane, and carane-was synthesized byŚcianowski and co-workers [83]. The compounds were tested as antioxidants and anticancer agents. The best antioxidant activity and the highest antiproliferative potential were observed for αand β-pinane N-substituted benzisoselenazolones 89 and 90 ( Figure 20). The N-isopinocampheyl-1,2-benzisoselenazol-3(2H)-one 89 is the best peroxide scavenger and antiproliferative agent on the human promyelocytic leukemia cell line HL-60 with IC 50 of 7.1 µM (HL-60 cell line).
Lin et al. [84] presented the efficient synthesis of α-pinene-based dithiadiazoles 92 by the cyclization reaction of α-pinene-based disubstituted phenyl acyl amino thioureas 91. The experiments showed that both compounds have weak herbicidal activity and the inhibition rates against fungi (Brassica campestris L., Physalospora piricola) are moderate except for 2,2 -methyl-substituted derivatives. A better plant growth-regulating activity was observed for derivatives with halo groups as compared to the other functional groups (Figure 21).
Lin et al. [84] presented the efficient synthesis of α-pinene-based dithiadiazoles 92 by the cyclization reaction of α-pinene-based disubstituted phenyl acyl amino thioureas 91. The experiments showed that both compounds have weak herbicidal activity and the inhibition rates against fungi (Brassica campestris L., Physalospora piricola) are moderate except for 2,2′-methyl-substituted derivatives. A better plant growth-regulating activity was observed for derivatives with halo groups as compared to the other functional groups (Figure 21).

Camphor
Camphor, 1,7,7-trimethylbicyclo[2.2.1]heptan-2-one, is an abundant monoterpenoid with a bicyclic framework structure. This inexpensive natural compound occurs in dextrorotatory form, i.e., R,R-(+)-camphor, and was one of the first plant metabolites isolated in the chemically pure form [85]. Nowadays, it is obtained through distillation of camphor laurel tree wood (Cinnamomum camphora (L.) J.Presl) or otherwise by chemical transformation of other natural products, among other turpentines [86]. The laevorotatory form (S,S-camphor) only exists in a synthetic form or very small quantities in specific species of plants [87]. Camphor has been used widely as a fragrance in cosmetics and perfumes, a food flavorant, and in household cleaners [86]. This bicyclic compound possesses many biological activities: Insecticidal, analgesic, antimicrobial, antiviral, anticoccidial, antinociceptive, anticancer, and antitussive [86,88]. Importantly, (+)-camphor is one of the most commercially important aroma chemicals with an annual market value of US$80-100 million [89].
Imines based on natural (+)-camphor proved to be a promising source of antimicrobial and antiviral agents [90]. Yarovaya et al. showed the designing strategy of (+)-camphor derivatives with

Camphor
Camphor, 1,7,7-trimethylbicyclo[2.2.1]heptan-2-one, is an abundant monoterpenoid with a bicyclic framework structure. This inexpensive natural compound occurs in dextrorotatory form, i.e., R,R-(+)-camphor, and was one of the first plant metabolites isolated in the chemically pure form [85]. Nowadays, it is obtained through distillation of camphor laurel tree wood (Cinnamomum camphora (L.) J.Presl) or otherwise by chemical transformation of other natural products, among other turpentines [86]. The laevorotatory form (S,S-camphor) only exists in a synthetic form or very small quantities in specific species of plants [87]. Camphor has been used widely as a fragrance in cosmetics and perfumes, a food flavorant, and in household cleaners [86]. This bicyclic compound possesses many biological activities: Insecticidal, analgesic, antimicrobial, antiviral, anticoccidial, antinociceptive, anticancer, and antitussive [86,88]. Importantly, (+)-camphor is one of the most commercially important aroma chemicals with an annual market value of US$80-100 million [89].
Imines based on natural (+)-camphor proved to be a promising source of antimicrobial and antiviral agents [90]. Yarovaya et al. showed the designing strategy of (+)-camphor derivatives with the imine group ( Figure 22) was prepared based on significant structural data described by the authors and shows the elements of the molecule which affects biological activity [91]. the imine group ( Figure 22) was prepared based on significant structural data described by the authors and shows the elements of the molecule which affects biological activity [91]. The most common (+)-camphor-based imine is camphecene 93 (Figure 23), 2-(E)-((1R,4R)-1,7,7trimethylbicyclo[2.2.1]heptan-2-ylidene-aminoethanol, which was established as an effective inhibitor of influenza virus H1N1 with a selectivity index (SI) value of 500.3 [88] together with low toxicity. Camphecene remains promising as a potential antiviral due to the low pathogenicity of resistant viruses that may arise [92]. The most common (+)-camphor-based imine is camphecene 93 (Figure 23), 2-(E)-((1R,4R)-1,7,7trimethylbicyclo[2.2.1]heptan-2-ylidene-aminoethanol, which was established as an effective inhibitor of influenza virus H1N1 with a selectivity index (SI) value of 500.3 [88] together with low toxicity. Camphecene remains promising as a potential antiviral due to the low pathogenicity of resistant viruses that may arise [92]. The most common (+)-camphor-based imine is camphecene 93 (Figure 23), 2-(E)-((1R,4R)-1,7,7trimethylbicyclo[2.2.1]heptan-2-ylidene-aminoethanol, which was established as an effective inhibitor of influenza virus H1N1 with a selectivity index (SI) value of 500.3 [88] together with low toxicity. Camphecene remains promising as a potential antiviral due to the low pathogenicity of resistant viruses that may arise [92]. An interesting series of works on the topic of (+)-camphor-based imine derivatives is presented by Sokolova et al. [88,93,94]. The authors pointed up a very efficient antiviral activity of synthesized monoterpene derivatives. In their 2017 work [94], designed compounds were prepared from commercially available natural (+)-camphor and various amines (Figure 24), whereas the procedures for the monoterpene derivatives were simple and reproducible. The prepared compounds were tested in vitro for antiviral activity. Most of those (+)-camphor-based imine derivatives-especially 94 and 95-were capable of inhibiting the drug-resistant strains of influenza A virus subtypes A/Puerto Rico/8/34 and A/California/07/09 of H1N1pdm09. Analysis of the structure-activity relationship showed that the virus-inhibiting properties are strongly dependent on the length of an imine-conjugated moiety: Compounds with a shorter chain display higher activity, whereas the suppressing action of those aliphatic (+)-camphor imines with long aliphatic chains is lower.
Other (+)-camphor derivatives, having two bicyclic monoterpene elements, two imine groups, and additionally two quaternary atoms in their structures connected with various lengths of the alkyl chain ( Figure 25), were synthesized and tested through their biological applications [93]. Compounds with the pentyl 97 and nonyl 98 aliphatic chains are very efficient in inhibiting the influenza virus A(H1N1)pdm09 reproduction. Bis-quaternary ammonium bromide with a dodecyl alkyl chain 99 has the highest activity for the CEM-13 cells. The results suggest that tested dimeric bis-quaternary An interesting series of works on the topic of (+)-camphor-based imine derivatives is presented by Sokolova et al. [88,93,94]. The authors pointed up a very efficient antiviral activity of synthesized monoterpene derivatives. In their 2017 work [94], designed compounds were prepared from commercially available natural (+)-camphor and various amines (Figure 24), whereas the procedures for the monoterpene derivatives were simple and reproducible. The prepared compounds were tested in vitro for antiviral activity. Most of those (+)-camphor-based imine derivatives-especially 94 and 95-were capable of inhibiting the drug-resistant strains of influenza A virus subtypes A/Puerto Rico/8/34 and A/California/ 07/09 of H1N1pdm09. Analysis of the structure-activity relationship showed that the virus-inhibiting properties are strongly dependent on the length of an imine-conjugated moiety: Compounds with a shorter chain display higher activity, whereas the suppressing action of those aliphatic (+)-camphor imines with long aliphatic chains is lower.
Other (+)-camphor derivatives, having two bicyclic monoterpene elements, two imine groups, and additionally two quaternary atoms in their structures connected with various lengths of the alkyl chain ( Figure 25), were synthesized and tested through their biological applications [93]. Compounds with the pentyl 97 and nonyl 98 aliphatic chains are very efficient in inhibiting the influenza virus A(H1N1)pdm09 reproduction. Bis-quaternary ammonium bromide with a dodecyl alkyl chain 99 has the highest activity for the CEM-13 cells. The results suggest that tested dimeric bis-quaternary ammonium salts 97-99 might be used as components of drugs for suppressing malignant tumor development.       The next example, showing the use of naturally occurring camphor as a substrate, led to obtaining imines 100-102, and also hydrazones 103-105, which belong to R,R-(+)-camphor derivatives [95].  The next example, showing the use of naturally occurring camphor as a substrate, led to obtaining imines 100-102, and also hydrazones 103-105, which belong to R,R-(+)-camphor derivatives [95]. Figure 26     Sokolova et al. [97] presented the efficient synthesis of α-truxillic acid derivatives containing a monoterpenoid fragment. The authors used natural terpene, (+)-camphor, as a starting monoterpenoid because of its high biological activity and commercial availability. Novel (+)-camphor derivative of α-truxillic acid, namely 2,4-diphenyl-N 1 ,N 3 -bis((1R,4R)-1,7,7trimethylbicyclo[2.2.1]heptan-2-yl)cyclobutane-1,3-dicarboxamide) 112 ( Figure 28), was investigated for its analgesic activity in in vivo tests. The tested compound containing the cyclobutane unit and monoterpene bicyclic element at a dose of 10 mg/kg (per os) has good analgesic activity. Importantly, α-truxillic acid (tested for the same dose) did not show analgesic activity. Thus, the cause of Sokolova et al. [97] presented the efficient synthesis of α-truxillic acid derivatives containing a monoterpenoid fragment. The authors used natural terpene, (+)-camphor, as a starting monoterpenoid because of its high biological activity and commercial availability. Novel (+)-camphor derivative of α-truxillic acid, namely 2,4-diphenyl-N 1 ,N 3 -bis((1R,4R)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl) cyclobutane-1,3-dicarboxamide) 112 ( Figure 28), was investigated for its analgesic activity in in vivo tests. The tested compound containing the cyclobutane unit and monoterpene bicyclic element at a dose of 10 mg/kg (per os) has good analgesic activity. Importantly, α-truxillic acid (tested for the same dose) did not show analgesic activity. Thus, the cause of considerable analgesic activity occurring in the tested compound is the introduction of (+)-camphor 112 fragment into α-truxillic acid.

substituents.
Sokolova et al. [97] presented the efficient synthesis of α-truxillic acid derivatives containing a monoterpenoid fragment. The authors used natural terpene, (+)-camphor, as a starting monoterpenoid because of its high biological activity and commercial availability. Novel (+)-camphor derivative of α-truxillic acid, namely 2,4-diphenyl-N 1 ,N 3 -bis((1R,4R)-1,7,7trimethylbicyclo[2.2.1]heptan-2-yl)cyclobutane-1,3-dicarboxamide) 112 ( Figure 28), was investigated for its analgesic activity in in vivo tests. The tested compound containing the cyclobutane unit and monoterpene bicyclic element at a dose of 10 mg/kg (per os) has good analgesic activity. Importantly, α-truxillic acid (tested for the same dose) did not show analgesic activity. Thus, the cause of considerable analgesic activity occurring in the tested compound is the introduction of (+)-camphor 112 fragment into α-truxillic acid. Another example of the camphor amide derivatives is presented in Figure 29. Those heterocyclics were tested as potential vaccinia virus (VV) inhibitors [98]. Bioassay results revealed that synthesized amides with 4-methylpiperidine 113a and morpholine 114b fragments show the best inhibitory activity against VV with the IC50 values of 2.5 and 7.5 µM, respectively, with low cytotoxicity. Another example of the camphor amide derivatives is presented in Figure 29. Those heterocyclics were tested as potential vaccinia virus (VV) inhibitors [98]. Bioassay results revealed that synthesized amides with 4-methylpiperidine 113a and morpholine 114b fragments show the best inhibitory activity against VV with the IC 50 values of 2.5 and 7.5 µM, respectively, with low cytotoxicity. A series of hybrid molecules based on (+)-camphor derivatives and quinolizidine alkaloid (-)cytisine ( Figure 30) were presented by Artyushin et. al. [99] and tested for their cytotoxicity and virusinhibiting activity. It was proven that the antiviral activity is affected by the length and nature of linkers between cytisine and (+)-camphor moieties. Compound 117d, which contains the cytisine fragment separated from triazole ring by -C6H12-aliphatic linker shows the highest activity with relatively low toxicity (CC50 = 168 µmol, IC50 = 8 µmol, SI = 20). Its selectivity index appears higher than that of the commonly known reference compound: Rimantadine. A series of hybrid molecules based on (+)-camphor derivatives and quinolizidine alkaloid (-)-cytisine ( Figure 30) were presented by Artyushin et. al. [99] and tested for their cytotoxicity and virus-inhibiting activity. It was proven that the antiviral activity is affected by the length and nature of linkers between cytisine and (+)-camphor moieties. Compound 117d, which contains the cytisine fragment separated from triazole ring by -C 6 H 12 -aliphatic linker shows the highest activity with relatively low toxicity (CC 50 = 168 µmol, IC 50 = 8 µmol, SI = 20). Its selectivity index appears higher than that of the commonly known reference compound: Rimantadine.
A series of hybrid molecules based on (+)-camphor derivatives and quinolizidine alkaloid (-)cytisine ( Figure 30) were presented by Artyushin et. al. [99] and tested for their cytotoxicity and virusinhibiting activity. It was proven that the antiviral activity is affected by the length and nature of linkers between cytisine and (+)-camphor moieties. Compound 117d, which contains the cytisine fragment separated from triazole ring by -C6H12aliphatic linker shows the highest activity with relatively low toxicity (CC50 = 168 µmol, IC50 = 8 µmol, SI = 20). Its selectivity index appears higher than that of the commonly known reference compound: Rimantadine. (+)-Camphoric acid 120 (Figure 31), which is the product of oxidation of (+)-camphor, was a substrate for an effective technique for the one-stage synthesis of new polycyclic nitrogen-containing compounds [100]. In this work, various aliphatic and aromatic diamines were selected as reagents for target polycyclic molecules because of the considerable role in the medicinal and organic chemistry of nitrogen-containing heterocyclic compounds. In vitro screening for the activity of the influenza (+)-Camphoric acid 120 (Figure 31), which is the product of oxidation of (+)-camphor, was a substrate for an effective technique for the one-stage synthesis of new polycyclic nitrogen-containing compounds [100]. In this work, various aliphatic and aromatic diamines were selected as reagents for target polycyclic molecules because of the considerable role in the medicinal and organic chemistry of nitrogen-containing heterocyclic compounds. In vitro screening for the activity of the influenza virus A was performed for all synthesized polycyclic nitrogen-containing heterocyclic compounds, and synthetic analogs of natural alkaloids. Figure 31 shows selected structures (121)(122)(123)(124), which were most effective in inhibiting the influenza virus A (H1N1). Compound 124, exhibiting the best antivirus agent among others, has inhibitory activity against different strains of influenza virus A. This quinazoline like agent 124 has inhibitory activity against strain H5N2 comparable to reference compounds. In the case of effective inhibiting of strain H1N1, molecule 124 indicates an antivirus activity exceeding that of reference compounds. virus A was performed for all synthesized polycyclic nitrogen-containing heterocyclic compounds, and synthetic analogs of natural alkaloids. Figure 31 shows selected structures (121)(122)(123)(124), which were most effective in inhibiting the influenza virus A (H1N1). Compound 124, exhibiting the best antivirus agent among others, has inhibitory activity against different strains of influenza virus A. This quinazoline like agent 124 has inhibitory activity against strain H5N2 comparable to reference compounds. In the case of effective inhibiting of strain H1N1, molecule 124 indicates an antivirus activity exceeding that of reference compounds. Another example, where the (1S)-(+)-camphor-10-sulfonic acid was used as a precursor of Nheterocycle compounds, is the work where compounds from the group of (1S)-(+)-camphor-10- 128 is regarded as highly active against tested bacteria and fungi. The microbiological activity of this bromide 128 is approximately 25 times more effective against S. aureus and C. albicans and 100 times more active against E. coli than commercially used antimicrobial standard: Benzalkonium bromide.
Another example, where the (1S)-(+)-camphor-10-sulfonic acid was used as a precursor of N-heterocycle compounds, is the work where compounds from the group of (1S)-(+)-camphor-10sulfonamide derivatives 129 ( Figure 33) were investigated as antiviral inhibitors against filoviruses [102]. The derivatives bearing morpholine 129a and triazole 129g moieties exhibit the highest inhibitory activity towards the Ebola virus glycoprotein, with the effectiveness level comparable to that of the reference drug. The excellent results presented in this paper allow us to predict that subsequent monoterpene bicyclic derivatives might be efficient inhibitors of especially dangerous viral infections.

Borneol
Borneol (1,7,7-trimethylbicyclo[2.2.1]heptan-2-ol) is bicyclic monoterpenoid alcohol that exists as two enantiomers in the D and L forms that occur in the essential oils of numerous medicinal plants, such as valerian (Valeriana officinalis subsp. collina (Wallr.) Nyman), chamomile (Matricaria chamomilla L. Rydb.), and lavender (Lavandula officinalis Chaix). It has four configurations that correspond to different positions of the hydroxyl group. Borneol is used-among others-in perfume and cosmetics manufacturing, agriculture and wood, and pharmaceutical industries. In China's and India's traditional medicine, borneol has long since been used as a remedy against gastrointestinal diseases.

Borneol
Borneol (1,7,7-trimethylbicyclo[2.2.1]heptan-2-ol) is bicyclic monoterpenoid alcohol that exists as two enantiomers in the D and L forms that occur in the essential oils of numerous medicinal plants, such as valerian (Valeriana officinalis subsp. collina (Wallr.) Nyman), chamomile (Matricaria chamomilla L. Rydb.), and lavender (Lavandula officinalis Chaix). It has four configurations that correspond to different positions of the hydroxyl group. Borneol is used-among others-in perfume and cosmetics manufacturing, agriculture and wood, and pharmaceutical industries. In China's and India's traditional medicine, borneol has long since been used as a remedy against gastrointestinal diseases. (-)-Borneol and its derivatives are antimicrobial [103], anti-inflammatory [104], and antiviral [105]. Borneol has been shown to promote drugs crossing through the blood-brain barrier, thus improving the efficiency of these drugs [106].
(-)-Borneol esters are also found in a few plant families: For instance, the hydroxycinnamic variety in Eupatorium deltoideum Poepp. ex Spreng. and Piper caninum Blume. Such natural esters show a broad spectrum of biological properties. Bornyl caffeate and coumarate are highly antibacterial [110].
Bornyl salicylate-commonly known salicylic derivative, obtained by esterification of salicylic acid and (-)-borneol in the early 20th century-was used topically in inflammatory diseases. Interestingly, it was only in 2012 that the first detailed analysis of the anti-inflammatory effects of bornyl salicylate (BS) in experimental models of acute inflammation was done [104]. That research showed that signs of acute toxicity are not observed either in male or female mice. The authors revealed that the tested pharmaceutical compound is anti-inflammatory, which is related to the decrease in pro-inflammatory mediators because the treatment with bornyl salicylate is effective in the reduction of paw edema in both early and late phases.
A large set of monoterpene derivatives that belong to the group of (-)-borneol esters were designed, synthesized, and examined with a view to their antimicrobial activity [109]. Desired compounds were prepared by the conventional and microwave-assisted methodology and the obtained (-)-borneol derivatives contained various aliphatic and aromatic moieties ( Figure 34); all of them (130) were tested for their antibacterial and antifungal properties. Interestingly, among all examined monoterpene esters only those with methoxylated aromatic substituents (130a-c) show noteworthy microbiological results. The antimicrobial assays confirmed that compounds 130a, 130b, and 130c exhibit promising antibacterial activity for some microorganisms, which is comparable to commonly used standard-ampicillin (MIC 50 = 62.5 µM). Bornyl 3 ,4 -dimethoxybenzoate 130b shows high antimicrobial activity against all tested bacteria and fungus.
The presented set of aliphatic and aromatic esters of (-)-borneol derivatives ( Figure 34) was also tested by the same research group for their antiproliferative and antioedematogenic properties [108]. Compounds 130c and 130d show a pronounced cytostatic activity against various tumor cell lines, which makes them useful models for the development of alternative drugs that might be effective and highly promising in the treatment of cancer. Additionally, compound 130d [(1S,2R,4S)-1,7,7trimethylbicyclo[2.2.1]heptan-2-yl benzoate] shows potential antioedematogenic activity being effective in the reduction of oedematogenic response in all evaluated periods. This aromatic ester of (-)-borneol derivative might be useful in the development of new antiinflammatory drugs.
The presented set of aliphatic and aromatic esters of (-)-borneol derivatives ( Figure 34) was also tested by the same research group for their antiproliferative and antioedematogenic properties [108]. Compounds 130c and 130d show a pronounced cytostatic activity against various tumor cell lines, which makes them useful models for the development of alternative drugs that might be effective and highly promising in the treatment of cancer. Additionally, compound 130d [(1S,2R,4S)-1,7,7trimethylbicyclo[2.2.1]heptan-2-yl benzoate] shows potential antioedematogenic activity being effective in the reduction of oedematogenic response in all evaluated periods. This aromatic ester of (-)-borneol derivative might be useful in the development of new antiinflammatory drugs.
It is commonly known that heterocyclic compounds and their various derivatives due to their beneficial biological, pharmacological, and medical properties have attracted great attention from medicinal chemistry. Commonly mentioned examples are derivatives of benzimidazole, benzoxazole, and benzothiazole, which possess a wide range of biological effects, mainly antiulcer, anti-inflammatory, antiviral activity, antihypertensive, and analgesic [111][112][113][114]. On the other hand, the presence of 1,7,7trimethylbicyclo[2.2.1]heptane scaffold of designed molecules induces many biological activities of prepared compounds, for instance high inhibitory activity of molecules on the replication of influenza A virus [88,94,95]. Hence, works where the compounds containing those two structural elements are broadly tested and significant biological activity are demonstrated. Importantly, most of such structural features of terpene-heterocyclic molecules also possess ester linker ( Figure 37).
Another work showing a new (-)-borneol ester derivative [97] focuses on the efficient synthesis of bis((1R,4R)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl) 2,4-diphenylcyclobutane-1,3-dicarboxylate 131 using α-truxillic acid and monoterpenoid bicyclic (-)-borneol as the substrates (Figure 35). The new terpene compound obtained by Sokolova et al. exhibits a considerable analgesic activity. α-Truxillic acid (tested for the same dose) does not show analgesic activity, thus the monoterpenoid fragment is a structurally important element of the molecule, influencing its activity.  It is commonly known that heterocyclic compounds and their various derivatives due to their beneficial biological, pharmacological, and medical properties have attracted great attention from medicinal chemistry. Commonly mentioned examples are derivatives of benzimidazole, benzoxazole, and benzothiazole, which possess a wide range of biological effects, mainly antiulcer, antiinflammatory, antiviral activity, antihypertensive, and analgesic [111][112][113][114]. On the other hand, the presence of 1,7,7-trimethylbicyclo[2.2.1]heptane scaffold of designed molecules induces many biological activities of prepared compounds, for instance high inhibitory activity of molecules on the replication of influenza A virus [88,94,95]. Hence, works where the compounds containing those two structural elements are broadly tested and significant biological activity are demonstrated. Importantly, most of such structural features of terpene-heterocyclic molecules also possess ester linker ( Figure 37). hCES2 [10]. The tested ester derivative of natural occurring (-)-borneol with the hydrazinylidene group is more active and selective against hCES1 than against hCES2. This compound inhibits hCES1 with the IC50 values of 0.098 ± 0.008 µM and has inhibitory activities against hCES2 with an IC50 value of 2.10 ± 0.18 µM. It is commonly known that heterocyclic compounds and their various derivatives due to their beneficial biological, pharmacological, and medical properties have attracted great attention from medicinal chemistry. Commonly mentioned examples are derivatives of benzimidazole, benzoxazole, and benzothiazole, which possess a wide range of biological effects, mainly antiulcer, antiinflammatory, antiviral activity, antihypertensive, and analgesic [111][112][113][114]. On the other hand, the presence of 1,7,7-trimethylbicyclo[2.2.1]heptane scaffold of designed molecules induces many biological activities of prepared compounds, for instance high inhibitory activity of molecules on the replication of influenza A virus [88,94,95]. Hence, works where the compounds containing those two structural elements are broadly tested and significant biological activity are demonstrated. Importantly, most of such structural features of terpene-heterocyclic molecules also possess ester linker ( Figure 37).  One of the works on this subject is an article studying various heterocyclic (-)-borneol derivatives with benzothiazole, benzoxazole, benzimidazole, and other heterocycles moieties ( Figure 38) [105].
Those compounds were analyzed due to their biological properties, including their possible use as antiviral, antiulcer, and analgesic agents. The inhibition of the influenza virus replication was evaluated with the A/Puerto Rico/8/34 (H1N1) virus, while rimantadine, amantadine, and deitiforin were tested as reference compounds, due to the structural similarity of their rigid cage fragments relative to heterocyclic (-)-borneol derivatives. Compound bearing two terpene moieties attached to 1H-benzimidazole-2-thiol core 133b (Figure 38) exhibit considerable antiviral properties. Compounds with 1-methyl-1H-imidazole-2(3H)-thione 133c and 1,2,4-triazole 133d (Figure 38) fragments should be included as heterocyclic (-)-borneol derivatives exhibiting high antiviral activity. Compound 133a containing benzoxazole-2-thiol fragment shows significant antiulcer activity. The examination of the gastric mucosa of the animals treated with 133a (-)-borneol derivative prior to the indomethacin administration demonstrated a considerably reduced ulceration rate (PI 0.8) in comparison with the animals of the control group (PI 5.0). The results indicated that the antiulcer activity score of compound 133a (AA with the value of 6.5) was higher than the same score for the reference compound (AA with a value of 3.  Another example presents various (-)-borneol and (-)-isoborneol (with different stereochemistries of the hydroxyl group) derivatives bearing a morpholine fragment as potential inhibitors of the influenza A virus (Figure 39) [115]. Compounds 134, 135, and 137 exhibit particularly high antiviral activity against the influenza virus A/Puerto Rico/8/34 (H1N1). The structure-activity analysis of those heterocyclic (-)-borneol derivatives revealed that a bulky lipophilic unit in the form of 1,7,7-trimethylbicyclo[2.2.1]heptan scaffold is required to obtain high results of antiviral activity.    [116]. Some selected structures of the prepared set are shown in Figure 40. Compounds 140 and 142 containing piperazine moiety demonstrate antiulcerogenic activity on the indomethacin-induced ulcer model. Moreover, those (-)-bornyl ester derivatives possess a considerable antiulcerogenic effect, which is comparable to that shown by Omeprazole and Famotidine, used as standards. Bornyl 2-piperazinoacetate with a non-substituted piperazine moiety 140 shows maximum antiulcerogenic activity. Furthermore, this ester has a significant gastroprotective effect on the ethanol-induced ulcer model, which was similar to the one presented by Omeprazole. Therefore, further biological research into this (-)-borneol derivative containing piperazine moiety 140 should be aimed at the implementation of this compound as an antiulcerogenic agent.  Over 170 natural derivatives were screened in search of currently no approved antiviral therapy Another work presents (-)-bornyl ester derivatives with N-containing heterocycles as vaccinia virus (VV) inhibitors [98]. Some of the structures of the large set designed and synthesized by Sokolova et al. are shown in Figure 41. Bioassay results confirmed that four (-)-borneol derivatives (143, 144) exhibit the best inhibitory activity against VV and low cytotoxicity. The authors emphasized two important structural issues that affect high anti-VV activity. Firstly, SAR studies suggest that the incorporation of the N-heterocyclic unit into the 1,7,7-trimethylbicyclo[2.2.1]heptane scaffold caused a significant improvement to antiviral activity. Secondly, (-)-borneol does not exhibit activity against VV. Thus, the presence of both heterocyclic elements and 1,7,7-trimethylbicyclo[2.2.1]heptane fragment is responsible for the antiviral activity against VV. The effectiveness of presented esters (Figure 41, compounds 143 and 144) is comparable to the reference drug Cidofovir and suggests that those (-)-borneol heterocycle derivatives should be considered as potential anti-VV drug candidates.  Over 170 natural derivatives were screened in search of currently no approved antiviral therapy of Marburg virus disease (MVD) [117]. The (-)-bornyl ester derivatives containing saturated Nheterocycles exhibited the highest antiviral activity and can specifically inhibit MarV entry with the efficiency being comparable to that of the previously described verapamil. (-)-Borneol itself is not active against both tested pseudotypes. Among low-toxic (-)-borneol derivatives, some of the compounds inter alia 138-141 turned out to be relatively specific inhibitors of MarV-GP mediated infection (SC > 10). Compound 139 containing a methylpiperidine moiety exhibits the highest virusspecific activity, which is twice as high as that of the reference.
Biological activities were also determined for another structural type of derivatives of (-)borneol. One of the examples is 1,7,7-trimethylbicyclo[2.2.1]hept-2-yl methane sulfonate 145 ( Figure  42), obtained from the reaction of (1S)-endo-(-)-borneol with methanesulfonyl chloride [118]. The antibacterial effect of compound 145 is comparable with that of the commonly used referencechloramphenicol. Terpene compounds might also be used as substrates for the synthesis of specific polymer biomaterials as effective fouling resistant materials with lower cytotoxicity. An example of such an application is the utilization of natural borneol in designing a novel antibacterial material and developing an advanced strategy for antibacterial adhesion through polymer surface stereochemistry [119]. The authors used endo-L-borneol: (1S,2R,4S), endo-D-borneol: (1R,2S,4R), and exo-isoborneol: (1S,2S,4S) and (1R,2R,4R) to prepare a series of borneol-based polymer polyborneolacrylates (PBAs) (Figure 43, compounds 146) that showed unique antibacterial adhesion properties and significantly reduced bacterial attachment and biofilm formation. The PBA polymers were evaluated as Over 170 natural derivatives were screened in search of currently no approved antiviral therapy of Marburg virus disease (MVD) [117]. The (-)-bornyl ester derivatives containing saturated N-heterocycles exhibited the highest antiviral activity and can specifically inhibit MarV entry with the efficiency being comparable to that of the previously described verapamil. (-)-Borneol itself is not active against both tested pseudotypes. Among low-toxic (-)-borneol derivatives, some of the compounds inter alia 138-141 turned out to be relatively specific inhibitors of MarV-GP mediated infection (SC > 10). Compound 139 containing a methylpiperidine moiety exhibits the highest virus-specific activity, which is twice as high as that of the reference.
Biological activities were also determined for another structural type of derivatives of (-)-borneol. One of the examples is 1,7,7-trimethylbicyclo[2.2.1]hept-2-yl methane sulfonate 145 (Figure 42), obtained from the reaction of (1S)-endo-(-)-borneol with methanesulfonyl chloride [118]. The antibacterial effect of compound 145 is comparable with that of the commonly used reference-chloramphenicol.  Over 170 natural derivatives were screened in search of currently no approved antiviral therapy of Marburg virus disease (MVD) [117]. The (-)-bornyl ester derivatives containing saturated Nheterocycles exhibited the highest antiviral activity and can specifically inhibit MarV entry with the efficiency being comparable to that of the previously described verapamil. (-)-Borneol itself is not active against both tested pseudotypes. Among low-toxic (-)-borneol derivatives, some of the compounds inter alia 138-141 turned out to be relatively specific inhibitors of MarV-GP mediated infection (SC > 10). Compound 139 containing a methylpiperidine moiety exhibits the highest virusspecific activity, which is twice as high as that of the reference.
Biological activities were also determined for another structural type of derivatives of (-)borneol. One of the examples is 1,7,7-trimethylbicyclo[2.2.1]hept-2-yl methane sulfonate 145 ( Figure  42), obtained from the reaction of (1S)-endo-(-)-borneol with methanesulfonyl chloride [118]. The antibacterial effect of compound 145 is comparable with that of the commonly used referencechloramphenicol. Terpene compounds might also be used as substrates for the synthesis of specific polymer biomaterials as effective fouling resistant materials with lower cytotoxicity. An example of such an application is the utilization of natural borneol in designing a novel antibacterial material and developing an advanced strategy for antibacterial adhesion through polymer surface stereochemistry [119]. The authors used endo-L-borneol: (1S,2R,4S), endo-D-borneol: (1R,2S,4R), and exo-isoborneol: (1S,2S,4S) and (1R,2R,4R) to prepare a series of borneol-based polymer polyborneolacrylates (PBAs) (Figure 43, compounds 146) that showed unique antibacterial adhesion properties and significantly reduced bacterial attachment and biofilm formation. The PBA polymers were evaluated as Terpene compounds might also be used as substrates for the synthesis of specific polymer biomaterials as effective fouling resistant materials with lower cytotoxicity. An example of such an application is the utilization of natural borneol in designing a novel antibacterial material and developing an advanced strategy for antibacterial adhesion through polymer surface stereochemistry [119]. The authors used endo-L-borneol: (1S,2R,4S), endo-D-borneol: (1R,2S,4R), and exo-isoborneol: (1S,2S,4S) and (1R,2R,4R) to prepare a series of borneol-based polymer polyborneolacrylates (PBAs) (Figure 43, compounds 146) that showed unique antibacterial adhesion properties and significantly reduced bacterial attachment and biofilm formation. The PBA polymers were evaluated as noncytotoxic and should be considered a great potential for many biomedical applications. Superior antibacterial adhesion properties result from the borneol isomers on the material surface. This proof-of-concept improves polymer surface stereochemistry is an advanced strategy for antimicrobial adhesion, and the use of natural monoterpenes for this purpose is highly advisable. This concept is confirmed by the other work done by the Wang group [103], who presented (-)-borneol grafting onto cellulose employing chloroacetyl chloride as a covalent linking agent in a two-step reaction. The obtained borneol-grafted cellulose (BGC) (Figure 43, compound 147) material exhibits remarkable performance in antifungal adhesion and fungal growth inhibition, which suggests that grafted borneol moieties crucially influence the tactile sensing of fungal cells and, subsequently, their selectivity in adhesion. The simple modification consisting of adding small borneol molecules onto cellulose resulted in a significant conversion of the interfacial antifungal property and also opened a new opportunity for developing a new generation of antimicrobial celluloses. More crucially, both borneol and cellulose are natural products, therefore it should be concluded that it is possible to develop an advanced natural strategy for exploiting cellulose's potential use in antifungal adhesion and colonization. This environmentally friendly material might be applied in biomedicine and for sanitary purposes. noncytotoxic and should be considered a great potential for many biomedical applications. Superior antibacterial adhesion properties result from the borneol isomers on the material surface. This proofof-concept improves polymer surface stereochemistry is an advanced strategy for antimicrobial adhesion, and the use of natural monoterpenes for this purpose is highly advisable. This concept is confirmed by the other work done by the Wang group [103], who presented (-)borneol grafting onto cellulose employing chloroacetyl chloride as a covalent linking agent in a twostep reaction. The obtained borneol-grafted cellulose (BGC) (Figure 43, compound 147) material exhibits remarkable performance in antifungal adhesion and fungal growth inhibition, which suggests that grafted borneol moieties crucially influence the tactile sensing of fungal cells and, subsequently, their selectivity in adhesion. The simple modification consisting of adding small borneol molecules onto cellulose resulted in a significant conversion of the interfacial antifungal property and also opened a new opportunity for developing a new generation of antimicrobial celluloses. More crucially, both borneol and cellulose are natural products, therefore it should be concluded that it is possible to develop an advanced natural strategy for exploiting cellulose's potential use in antifungal adhesion and colonization. This environmentally friendly material might be applied in biomedicine and for sanitary purposes.
Sun et al. [120] described borneol-modified poly(methyl methacrylate) PMMA based on a facile and effective stereochemical strategy, generating an antibacterial copolymer named P(MMA-co-BA)-compound 148 ( Figure 43). This copolymer, which effectively prevents bacterial adhesion and is environmentally benign, might be successfully applied for the prevention of bacterial colonization in biomedical devices. Its unique antibacterial character depends on the percentage of BA segments in the copolymer.
The borneol-based polymers are hydrophobic and noncytotoxic, which can significantly improve the cell adhesion ability [121,122]; on the other hand, borneol isomers on the material surface contribute to the surface bacterial resistance properties [119,120]. Due to those data, Meng et al. [123] proposed polymerization with borneol based monomer, assuming that the hydrophobicity of zwitterionic polymers might increase, which will promote their cell adhesion ability without Sun et al. [120] described borneol-modified poly(methyl methacrylate) PMMA based on a facile and effective stereochemical strategy, generating an antibacterial copolymer named P(MMA-co-BA)compound 148 (Figure 43). This copolymer, which effectively prevents bacterial adhesion and is environmentally benign, might be successfully applied for the prevention of bacterial colonization in biomedical devices. Its unique antibacterial character depends on the percentage of BA segments in the copolymer.
The borneol-based polymers are hydrophobic and noncytotoxic, which can significantly improve the cell adhesion ability [121,122]; on the other hand, borneol isomers on the material surface contribute to the surface bacterial resistance properties [119,120]. Due to those data, Meng et al. [123] proposed polymerization with borneol based monomer, assuming that the hydrophobicity of zwitterionic polymers might increase, which will promote their cell adhesion ability without compromising their bacterial resistance properties. For this purpose, they synthesized hydrophobized zwitterionic copolymer P-(SBMA-co-DMA-co-ISA) (PSDI) (compound 149, Figure 43), containing borneol based segment isobornyl acrylate (ISA) and zwitterionic moiety SBMA was prepared by sulfonation of P(DMA-co-ISA) (PDI). Together with tannic acid (TA), PSDI colloidal particles (CPs) were deposited onto the Ti alloy surface by electrophoretic deposition to form zwitterionic-based coatings. Due to the synergistic effect between the SBMA and chiral ISA functional units, tested composite coatings can effectively inhibit the adhesion of Gram-negative (E. coli) and -positive bacteria (S. aureus). Furthermore, cells can adhere and grow on the surface of CP-TA composite coating as a result of the appropriate hydrophobicity provided by the chiral monoterpene moiety. Meng et al., in their work, presented a new strategy for preparing zwitterionic-based multifunctional coating. The proposed composite coatings have great potential for metal implant surface modification to prevent bacterial infections.
Another example showed the development of stereochemical antimicrobial strategy by grafting (-)-borneol 4-formylbenzoate to chitosan using a stable Schiff base bond (compound 150, Figure 43) [124]. The authors of that project received borneol-modified chitosan (BMC) as a potentially novel antimicrobial material. The biological tests were performed using Gram-negative E. coli, Gram-positive B. subtilis, and A. niger. BMC exhibits good antibacterial adhesion properties against both Gram-positive and Gramnegative bacteria. The BMC materials also demonstrate excellent antifungal adhesion performance (even for up to 29 days). Hence, modified chitosan with monoterpene moiety should be considered a broad-spectrum antimicrobial material. It enhances the protective effects of CF concerning the potential attachment of pathogens and maintains the skin flora. Therefore, BMC could have applications as a novel biomaterial-based on the stereochemical antimicrobial strategy and be useful in many biomedical and sanitary applications.
Another concept for creating an interesting biomaterial based on the (-)-borneol component is graphene oxide-borneol (GOB) composite [125], which can be synthesized by esterification of (-)-borneol with thiomalic-acid-modified graphene oxide (GO) sheets, of which thiomalic acid is used as the linker molecule to increase the number of surface carboxyl groups. GOB exhibits antifungal adhesion and growth inhibition. It was proven that the carbon stereochemistry of the GOB was essential for the powerful antifungal performance while the covalent banding between GO and borneol molecules ensured its safe and long-term antifungal characteristics.

Conclusions
Terpenes make up the largest group of secondary metabolites (over 50,000 substances) and due to their naturally widespread occurrence, they have become central to research activities worldwide. It has been demonstrated that the use of the available and cheap monoterpenes as building blocks for designing and synthesizing new and effective agents to treat several diseases and some viral and bacterial infections is a promising and progressing direction in medicinal chemistry.
A crucial benefit from using described compounds is that their resource is almost inexhaustible, which has also ensured their wide application in industry. Moreover, the use of monoterpenoids to design drugs often reduces the toxicity of the resulting compound, which is an important factor when considering the possible utilization and manufacture of new compounds. It is widely known that chemical modification of natural substances with various components is an important method for obtaining new biologically active compounds. This feature is particularly visible in the monoterpene derivatives presented in this review. Their biological activities are often much superior to the parent substances (monoterpenes); furthermore, they often exceed the commonly used standards in specialized areas of research.
The increased strength of activity is observed by supplement functionalized moiety (e.g., heterocyclic unit introduced into thymol, camphor, or borneol derivatives) or special group, like an ester group in borneol derivatives, or an imino group in camphor derivatives. Nowadays, this is a promising route as this is of utmost importance to utilize natural substances for discovering new compounds and to search for practical uses for the newly obtained materials. As presented in this paper, monoterpenes exhibit a large variety of biological activities that can be exploited in various branches of medicine and pharmacy. This review highlights the use of the selected beneficial monoterpenes and their derivatives for the treatment of various diseases. It should be mentioned that alongside the biological activities presented in this paper, the substances play important roles in preventing and remediating other diseases such as those of the heart and diabetes. Obtaining new structures based on monoterpene compounds is a dynamically developing area, and one should expect new interesting biological applications, as well as modifications to structures that will improve current results in biological research.