In Search of a Phosphorus Dendrimer-Based Carrier of Rose Bengal: Tyramine Linker Limits Fluorescent and Phototoxic Properties of a Photosensitizer
Abstract
:1. Introduction
2. Results
2.1. Synthesis and Characterization of Conjugates
2.2. Spectroscopy Studies
2.3. Size and Zeta Potential Measurements
2.4. Drug Release Studies
2.5. Cellular Uptake Assay
2.6. Singlet Oxygen Generation
2.7. Phototoxicity
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Methods
4.2.1. Preparation of Drug–Dendrimer Conjugates—General Information
4.2.2. Synthesis of the Rose Bengal Acid Chloride
4.2.3. Synthesis of Rose Bengal–Tyramine
4.2.4. Synthesis of Rose Bengal–G1, G2, G3 Phosphorus Dendrimers—Pyrrolidine Conjugates
Preparation of the PS-Cl2 G1, G2, G3 Dendrimer Conjugates Containing Three Molecules of Rose Bengal
Preparation of the PS-Cl2 G1, G2, G3 Dendrimer Conjugates Containing Three Molecules of Rose Bengal and 9, 21 and 45 Molecules of Pyrrolidine, Respectively
4.2.5. Spectroscopy Studies
4.2.6. Size and Zeta Potential Measurements
4.2.7. Drug Release Studies
4.2.8. Cell Culture
4.2.9. Cellular Uptake Determination
4.2.10. Singlet Oxygen Generation Assay
4.2.11. Cytotoxicity Studies
4.2.12. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Hydrodynamic Diameter (nm) | Zeta Potential (mV) | |
---|---|---|
G3–3RB | 18.17 ± 4.96 | 44.54 ± 2.42 |
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Sztandera, K.; Marcinkowska, M.; Gorzkiewicz, M.; Janaszewska, A.; Laurent, R.; Zabłocka, M.; Mignani, S.; Majoral, J.P.; Klajnert-Maculewicz, B. In Search of a Phosphorus Dendrimer-Based Carrier of Rose Bengal: Tyramine Linker Limits Fluorescent and Phototoxic Properties of a Photosensitizer. Int. J. Mol. Sci. 2020, 21, 4456. https://doi.org/10.3390/ijms21124456
Sztandera K, Marcinkowska M, Gorzkiewicz M, Janaszewska A, Laurent R, Zabłocka M, Mignani S, Majoral JP, Klajnert-Maculewicz B. In Search of a Phosphorus Dendrimer-Based Carrier of Rose Bengal: Tyramine Linker Limits Fluorescent and Phototoxic Properties of a Photosensitizer. International Journal of Molecular Sciences. 2020; 21(12):4456. https://doi.org/10.3390/ijms21124456
Chicago/Turabian StyleSztandera, Krzysztof, Monika Marcinkowska, Michał Gorzkiewicz, Anna Janaszewska, Regis Laurent, Maria Zabłocka, Serge Mignani, Jean Pierre Majoral, and Barbara Klajnert-Maculewicz. 2020. "In Search of a Phosphorus Dendrimer-Based Carrier of Rose Bengal: Tyramine Linker Limits Fluorescent and Phototoxic Properties of a Photosensitizer" International Journal of Molecular Sciences 21, no. 12: 4456. https://doi.org/10.3390/ijms21124456