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Article

Quantitative Trait Locus Mapping of Clubroot Resistance and Plasmodiophora brassicae Pathotype Banglim-Specific Marker Development in Brassica rapa

1
Molecular Genetics and Genomics Laboratory, Department of Horticulture, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, Korea
2
School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK
3
Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, Korea
4
Bio-Breeding Institute, 153-60 Sinryeong-ro, Anseong 17544, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(11), 4157; https://doi.org/10.3390/ijms21114157
Received: 20 April 2020 / Revised: 28 May 2020 / Accepted: 6 June 2020 / Published: 10 June 2020
(This article belongs to the Collection Genetics and Molecular Breeding in Plants)
Clubroot resistance is an economically important trait in Brassicaceae crops. Although many quantitative trait loci (QTLs) for clubroot resistance have been identified in Brassica, disease-related damage continues to occur owing to differences in host variety and constant pathogen variation. Here, we investigated the inheritance of clubroot resistance in a double haploid population developed by crossing clubroot resistant and susceptible lines “09CR500” and “09CR501”, respectively. The resistance of “09CR500” to Plasmodiophora brassicae pathotype “Banglim” was controlled as a single dominant gene, with the segregation of resistance and susceptibility being nearly 1:1. PbBrA08Banglim was identified as having a logarithm of odds value of 7.9–74.8, and a phenotypic variance of 26.0–97.1% with flanking marker “09CR.11390652” in A08. After aligning QTL regions to the B. rapa reference genome, 11 genes were selected as candidates. PbBrA08Banglim was located near Crr1, CRs, and Rcr9 loci, but differences were validated by marker analysis, gene structural variations, and gene expression levels, as well as phenotypic responses to the pathotype. Genotyping using the “09CR.11390652” marker accurately distinguished the Banglim-resistance phenotypes in the double haploid population. Thus, the developed marker will be useful in Brassica breeding programs, marker-assisted selection, and gene pyramiding to identify and develop resistant cultivars. View Full-Text
Keywords: clubroot disease; resistance mapping; quantitative trait loci (QTL); marker; Brassica rapa clubroot disease; resistance mapping; quantitative trait loci (QTL); marker; Brassica rapa
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MDPI and ACS Style

Choi, S.R.; Oh, S.H.; Chhapekar, S.S.; Dhandapani, V.; Lee, C.Y.; Rameneni, J.J.; Ma, Y.; Choi, G.J.; Lee, S.-S.; Lim, Y.P. Quantitative Trait Locus Mapping of Clubroot Resistance and Plasmodiophora brassicae Pathotype Banglim-Specific Marker Development in Brassica rapa. Int. J. Mol. Sci. 2020, 21, 4157. https://doi.org/10.3390/ijms21114157

AMA Style

Choi SR, Oh SH, Chhapekar SS, Dhandapani V, Lee CY, Rameneni JJ, Ma Y, Choi GJ, Lee S-S, Lim YP. Quantitative Trait Locus Mapping of Clubroot Resistance and Plasmodiophora brassicae Pathotype Banglim-Specific Marker Development in Brassica rapa. International Journal of Molecular Sciences. 2020; 21(11):4157. https://doi.org/10.3390/ijms21114157

Chicago/Turabian Style

Choi, Su R., Sang H. Oh, Sushil S. Chhapekar, Vignesh Dhandapani, Chang Y. Lee, Jana J. Rameneni, Yinbo Ma, Gyung J. Choi, Soo-Seong Lee, and Yong P. Lim. 2020. "Quantitative Trait Locus Mapping of Clubroot Resistance and Plasmodiophora brassicae Pathotype Banglim-Specific Marker Development in Brassica rapa" International Journal of Molecular Sciences 21, no. 11: 4157. https://doi.org/10.3390/ijms21114157

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