Molecular and Structural Basis of the Proteasome α Subunit Assembly Mechanism Mediated by the Proteasome-Assembling Chaperone PAC3-PAC4 Heterodimer

The 26S proteasome is critical for the selective degradation of proteins in eukaryotic cells. This enzyme complex is composed of approximately 70 subunits, including the structurally homologous proteins α1–α7, which combine to form heptameric rings. The correct arrangement of these α subunits is essential for the function of the proteasome, but their assembly does not occur autonomously. Assembly of the α subunit is assisted by several chaperones, including the PAC3-PAC4 heterodimer. In this study we showed that the PAC3-PAC4 heterodimer functions as a molecular matchmaker, stabilizing the α4-α5-α6 subcomplex during the assembly of the α-ring. We solved a 0.96-Å atomic resolution crystal structure for a PAC3 homodimer which, in conjunction with nuclear magnetic resonance (NMR) data, highlighted the mobility of the loop comprised of residues 51 to 61. Based on these structural and dynamic data, we created a three-dimensional model of the PAC3-4/α4/α5/α6 quintet complex, and used this model to investigate the molecular and structural basis of the mechanism of proteasome α subunit assembly, as mediated by the PAC3-PAC4 heterodimeric chaperone. Our results provide a potential basis for the development of selective inhibitors against proteasome biogenesis.


Introduction
The selective degradation of proteins in eukaryotic cells is essential for the maintenance of physiological homeostasis. Protein degradation is implemented primarily via the ubiquitin-proteasome system [1,2]. The proteasome is huge protein complex (26S), comprised of a 20S core particle (CP) and one or two 19S regulatory particles (RPs). The 20S CP, which has proteolytic activity, is composed of seven homologous α subunits, α1-α7, and seven homologous β subunits, β1-β7, which are assembled into a cylindrical structure with an α 1-7 β 1-7 β 1-7 α 1-7 arrangement. The 19S RP is responsible for the

The PAC3-PAC4 Heterodimer Interacts Primarily with α5
To study the biochemical processes involved in proteasome α-subunit assembly mediated by the PAC3-PAC4 heterodimer, we prepared all of the human proteasome α subunits as recombinant proteins. Although protocols to prepare PAC3 and PAC4 as individual recombinant proteins have been reported previously, their heterodimer is rather unstable, unlike the yeast orthologs Pba3 and Pba4 [16,18]. The recombinant PAC4 also has a tendency to form a domain-swapped homodimer [18].
To overcome these problems, we designed and prepared a PAC3-PAC4 heterodimer as a single-chain form, termed scPAC3/4, in which the C-terminus of PAC4 is connected to the N-terminus of PAC3 via a (GGGS) 4 liner. All of the recombinant proteins were produced using bacterial expression systems in Escherichia coli, and were successfully purified to homogeneity ( Figure S1).
To determine which proteasomal α subunits interact with the PAC3-PAC4 heterodimer, we performed in vitro pull-down experiments using these recombinant proteins. In the pull-down assay, His 6 -tagged scPAC3/4 was applied to Ni 2+ -charged resin, and subsequently incubated with a mixture of all of the α-subunit proteins. Since α7 spontaneously forms an oligomer that is capable of capturing α6 [19,20], we carried out this experiment both in the absence and in the presence of α7. The pull-down experiments showed that scPAC3/4 reacted with several α subunits including α4, α5, and α6 ( Figure 1). Addition of α7 had virtually no impact on the interaction of α6 with scPAC3/4, suggesting that it has a higher affinity for the PAC3-PAC4 heterodimer than for the α7 oligomer. To avoid ambiguity due to overlapping of the Coomassie Brilliant Blue (CBB)-stained bands, we performed the pull-down experiments using α1, α4, α5, and α6 individually. The pull-down assay showed that scPAC3/4 interacted most strongly with α5 and weakly with α4 and α6. By contrast, no interaction was detected between scPAC3/4 and α1. The interaction between α6 and scPAC3/4 appeared to be enhanced in the presence of the other α subunits. Since α5 and α6 occur consecutively in the native α ring, these data suggest that the PAC3-PAC4 heterodimer is important for α5-α6 subcomplex assembly during proteasome α-ring formation. His6-tagged scPAC3/4 was applied to Ni 2+ -charged resin, and subsequently incubated with a mixture of all of the α-subunit proteins. Since α7 spontaneously forms an oligomer that is capable of capturing α6 [19,20], we carried out this experiment both in the absence and in the presence of α7. The pulldown experiments showed that scPAC3/4 reacted with several α subunits including α4, α5, and α6 ( Figure 1). Addition of α7 had virtually no impact on the interaction of α6 with scPAC3/4, suggesting that it has a higher affinity for the PAC3-PAC4 heterodimer than for the α7 oligomer. To avoid ambiguity due to overlapping of the Coomassie Brilliant Blue (CBB)-stained bands, we performed the pull-down experiments using α1, α4, α5, and α6 individually. The pull-down assay showed that scPAC3/4 interacted most strongly with α5 and weakly with α4 and α6. By contrast, no interaction was detected between scPAC3/4 and α1. The interaction between α6 and scPAC3/4 appeared to be enhanced in the presence of the other α subunits. Since α5 and α6 occur consecutively in the native α ring, these data suggest that the PAC3-PAC4 heterodimer is important for α5-α6 subcomplex assembly during proteasome α-ring formation. The non-tagged α1-α3 and α5-α7 along with 3xFLAG-tagged α4 were mixed with His6-tagged scPAC3/4 immobilized on Ni 2+ -charged Chelating Sepharose beads. The 3xFLAG-tagged α4 was used to avoid the band overlap between α4 and scPAC3/4. After extensive washing, bound proteins were analyzed using CBB staining after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The 'Input' lane contained all α subunits and His6-scPAC3/4 (0.5 μg each). The SDS-PAGE bands were assigned according to Figure S1a, and the bands originating from the His6-scPAC3/4 and the bound α subunits are labeled.

The PAC3-PAC4 Heterodimer Acts as Molecular Matchmaker in α4-α5-α6 Assembly
In order to explore the functional mechanism of the PAC3-PAC4 heterodimer in proteasome assembly involving α4-α6, we investigated the inter-subunit interactions mediated by scPAC3/4. In a pull-down assay, glutathione S-transferase (GST)-fused α5 was used as a bait for probing its interactions with the other α subunits, both in the absence and in the presence of scPAC3/4. GST-α5 interacted weakly with α6 in the presence of scPAC3/4, while the other subunits were not reactive with α5 regardless of the presence or absence of scPAC3/4 ( Figure 2). In contrast, GST-α5 weakly interacted with α4 regardless of the presence or absence of scPAC3/4 under this assay condition. The results were not influenced by the presence of α7 (Figures 1 and 2). The non-tagged α1-α3 and α5-α7 along with 3xFLAG-tagged α4 were mixed with His 6 -tagged scPAC3/4 immobilized on Ni 2+ -charged Chelating Sepharose beads. The 3xFLAG-tagged α4 was used to avoid the band overlap between α4 and scPAC3/4. After extensive washing, bound proteins were analyzed using CBB staining after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The 'Input' lane contained all α subunits and His 6 -scPAC3/4 (0.5 µg each). The SDS-PAGE bands were assigned according to Figure S1a, and the bands originating from the His 6 -scPAC3/4 and the bound α subunits are labeled.

The PAC3-PAC4 Heterodimer Acts as Molecular Matchmaker in α4-α5-α6 Assembly
In order to explore the functional mechanism of the PAC3-PAC4 heterodimer in proteasome assembly involving α4-α6, we investigated the inter-subunit interactions mediated by scPAC3/4. In a pull-down assay, glutathione S-transferase (GST)-fused α5 was used as a bait for probing its interactions with the other α subunits, both in the absence and in the presence of scPAC3/4. GST-α5 interacted weakly with α6 in the presence of scPAC3/4, while the other subunits were not reactive with α5 regardless of the presence or absence of scPAC3/4 ( Figure 2). In contrast, GST-α5 weakly interacted with α4 regardless of the presence or absence of scPAC3/4 under this assay condition. The results were not influenced by the presence of α7 (Figures 1 and 2). Pull-down experiments between α5 and the other α subunits. The α1-α4, α6, and α7 subunits were mixed with GST-tagged α5 immobilized on Glutathione Sepharose beads. The 3xFLAG-tagged α4 and His6-tagged α6 were used to avoid the overlap of their bands with those of the other α subunits or scPAC3/4. (a) Interactions between α5 and the other α subunits in the presence and absence of the PAC3-PAC4 heterodimer. The 'Input1′ and 'Input2′ lanes contained His6-scPAC3/4 and α1-α6 subunits in the absence and presence of α7, respectively (0.5 μg each). (b) Interaction between α5 and the adjacent α subunits, α4 and α6. The 'Input3′ lane contained His6-scPAC3/4 and α4-α6 subunits. The pull-down experiment was also performed using an scPAC3/4 mutant with V77S and K80A substitutions in PAC3. Band assignments were carried out according to Figure S1b.
In yeast, the Pba3-Pba4 heterodimer acts as a matchmaker, reinforcing interactions between the α4 and α5 subunits [17]. The results of our pull-down analysis indicated that the PAC3-PAC4 heterodimer interacts with α4, α5, and α6, thereby acting as a molecular matchmaker for these proteasomal subunits. These findings suggest that the functional roles and interactions of this assembly chaperone complex with the proteasomal subunits are evolutionally conserved between yeast and humans.

Structural Insights into the Mechanisms Underlying PAC3/PAC4-Dependent α4-α5-α6 Assembly
To investigate the structural mechanisms underlying the chaperone-dependent formation of the α4-α5-α6 subcomplex, we built a three-dimensional model of the putative quintet complex comprised of PAC3, PAC4, α4, α5 and α6, using previously-reported crystallographic data. Crystal structures for the PAC3 homodimer [16], domain-swapped PAC4 homodimer [18], and 20S Pull-down experiments between α5 and the other α subunits. The α1-α4, α6, and α7 subunits were mixed with GST-tagged α5 immobilized on Glutathione Sepharose beads. The 3xFLAG-tagged α4 and His 6 -tagged α6 were used to avoid the overlap of their bands with those of the other α subunits or scPAC3/4. (a) Interactions between α5 and the other α subunits in the presence and absence of the PAC3-PAC4 heterodimer. The 'Input1 and 'Input2 lanes contained His 6 -scPAC3/4 and α1-α6 subunits in the absence and presence of α7, respectively (0.5 µg each). (b) Interaction between α5 and the adjacent α subunits, α4 and α6. The 'Input3 lane contained His 6 -scPAC3/4 and α4-α6 subunits. The pull-down experiment was also performed using an scPAC3/4 mutant with V77S and K80A substitutions in PAC3. Band assignments were carried out according to Figure S1b. In yeast, the Pba3-Pba4 heterodimer acts as a matchmaker, reinforcing interactions between the α4 and α5 subunits [17]. The results of our pull-down analysis indicated that the PAC3-PAC4 heterodimer interacts with α4, α5, and α6, thereby acting as a molecular matchmaker for these proteasomal subunits. These findings suggest that the functional roles and interactions of this assembly chaperone complex with the proteasomal subunits are evolutionally conserved between yeast and humans.

Structural Insights into the Mechanisms Underlying PAC3/PAC4-Dependent α4-α5-α6 Assembly
To investigate the structural mechanisms underlying the chaperone-dependent formation of the α4-α5-α6 subcomplex, we built a three-dimensional model of the putative quintet complex comprised of PAC3, PAC4, α4, α5 and α6, using previously-reported crystallographic data. Crystal structures for the PAC3 homodimer [16], domain-swapped PAC4 homodimer [18], and 20S proteasome [21] have been published. In addition, we newly determined a 0.96-Å high-resolution trigonal structure of the PAC3 homodimer ( Figure S2a). The overall structure of the trigonal form was very similar to that of the tetragonal structure we have previously published, except for a loop comprised of residues 51-61 ( Figure S2b), suggesting that it is mobile. Loop flexibility was also observed in the corresponding segment of the yeast ortholog Pba3 in its heterodimer with Pba4 [16] ( Figure S3). Our nuclear magnetic resonance (NMR) relaxation data from the human PAC3 homodimer confirmed that the loop is indeed mobile and disordered in solution ( Figure S4).
In the quintet-complex models, in addition to the interactions between PAC3-PAC4 and α5, based on the crystal structure of the yeast counterparts [16], the assembly chaperone interacted with the neighboring α4 and α6 subunits (Figure 3a). When the PAC3-4/α4/α5/α6 quintet complex model was superimposed onto the crystal structure of 20S CP, PAC3 and PAC4 make steric hindrance with β6 and β5, respectively, which possibly triggers the release of PAC3-4 from the α-ring upon binding of the β subunits onto the α-ring. A complex model, model A, based on the 2.00-Å PAC3 structure showed that the mobile loop was turned toward the solvent. Another model based on the 0.96-Å structure, model B, showed that the corresponding loop contacts α6. Apart from interactions involving this mobile loop, intermolecular contacts between the PAC3-PAC4 heterodimer and the proteasomal subunits are almost identical in the two models. Therefore, in the rest of this paper, we discuss the structural basis of the PAC3/PAC4-dependent α4-α5-α6 subunit assembly using model B. proteasome [21] have been published. In addition, we newly determined a 0.96-Å high-resolution trigonal structure of the PAC3 homodimer ( Figure S2a). The overall structure of the trigonal form was very similar to that of the tetragonal structure we have previously published, except for a loop comprised of residues 51-61 ( Figure S2b), suggesting that it is mobile. Loop flexibility was also observed in the corresponding segment of the yeast ortholog Pba3 in its heterodimer with Pba4 [16] ( Figure S3). Our nuclear magnetic resonance (NMR) relaxation data from the human PAC3 homodimer confirmed that the loop is indeed mobile and disordered in solution ( Figure S4). In the quintet-complex models, in addition to the interactions between PAC3-PAC4 and α5, based on the crystal structure of the yeast counterparts [16], the assembly chaperone interacted with the neighboring α4 and α6 subunits (Figure 3a). When the PAC3-4/α4/α5/α6 quintet complex model was superimposed onto the crystal structure of 20S CP, PAC3 and PAC4 make steric hindrance with β6 and β5, respectively, which possibly triggers the release of PAC3-4 from the α-ring upon binding of the β subunits onto the α-ring. A complex model, model A, based on the 2.00-Å PAC3 structure showed that the mobile loop was turned toward the solvent. Another model based on the 0.96-Å structure, model B, showed that the corresponding loop contacts α6. Apart from interactions involving this mobile loop, intermolecular contacts between the PAC3-PAC4 heterodimer and the proteasomal subunits are almost identical in the two models. Therefore, in the rest of this paper, we discuss the structural basis of the PAC3/PAC4-dependent α4-α5-α6 subunit assembly using model B. In this model, the interaction of α5 with the PAC3-PAC4 heterodimer is mediated by Gln70, Glu72, and Lys104 in PAC3, Arg48 in PAC4, and Glu95, His99, Tyr103, and Asp129 in α5 through In this model, the interaction of α5 with the PAC3-PAC4 heterodimer is mediated by Gln70, Glu72, and Lys104 in PAC3, Arg48 in PAC4, and Glu95, His99, Tyr103, and Asp129 in α5 through electrostatic interactions and hydrogen bonds (Figure 4). Our pull-down data indicated that scPAC3/4 interacted most strongly with α5 and weakly with α4 and α6 (Figure 1). The model predicted additional interactions between PAC3 and α6, and between PAC4 and α4 (Figure 4b,c). Specifically, Ser55, Lys80, and Asn81 of PAC3 form hydrogen bonds or electrostatic interactions with Ser110, Asp94, and Arg96 of α6, respectively. There are also predicted hydrophobic interactions of Val61, Phe85, and Val77 in PAC3 with Phe87, Phe97, and Leu93 in α6. Additionally, Asp70 and Arg85 of PAC4 have electrostatic interactions with Arg117 and Glu99 of α4, respectively (Figure 4c). Gln81 and Ile61 of PAC4 form hydrogen bonds and hydrophobic interactions with Ser93 and Val98 of α4, respectively.
To validate our docking model, we performed mutational experiments, especially focusing on the interaction between PAC3 and α6, which were specifically observed in the human proteins as compared with yeast counterparts. We constructed an scPAC3/PAC4 mutant in which putative α6-binding residues, Val77 and Lys80, of PAC3 are replaced with Ser and Ala, respectively. As expected, our mutational analysis indicated that mutations of Val77 and Lys80 of PAC3 impaired interaction with α6 but not with α4 and α5 (Figure 2b), confirming the validity of our docking model.  (Figure 4). Our pull-down data indicated that scPAC3/4 interacted most strongly with α5 and weakly with α4 and α6 (Figure 1). The model predicted additional interactions between PAC3 and α6, and between PAC4 and α4 (Figure 4b and  4c). Specifically, Ser55, Lys80, and Asn81 of PAC3 form hydrogen bonds or electrostatic interactions with Ser110, Asp94, and Arg96 of α6, respectively. There are also predicted hydrophobic interactions of Val61, Phe85, and Val77 in PAC3 with Phe87, Phe97, and Leu93 in α6. Additionally, Asp70 and Arg85 of PAC4 have electrostatic interactions with Arg117 and Glu99 of α4, respectively (Figure 4c). Gln81 and Ile61 of PAC4 form hydrogen bonds and hydrophobic interactions with Ser93 and Val98 of α4, respectively.
To validate our docking model, we performed mutational experiments, especially focusing on the interaction between PAC3 and α6, which were specifically observed in the human proteins as compared with yeast counterparts. We constructed an scPAC3/PAC4 mutant in which putative α6binding residues, Val77 and Lys80, of PAC3 are replaced with Ser and Ala, respectively. As expected, our mutational analysis indicated that mutations of Val77 and Lys80 of PAC3 impaired interaction with α6 but not with α4 and α5 (Figure 2b), confirming the validity of our docking model. Although the overall structures of human PAC3 and PAC4 are similar to those of yeast Pba3 and Pba4 (RMSD = 1.9-2.1Å and 1.9-2.2 Å) respectively, their amino acid sequence similarities are low (PAC3 versus Pba3 11.0%; PAC4 versus Pba4 14.6%). The α-subunit contacting residues of human PAC3 and PAC4, as predicted by the model, are not well-conserved in the yeast orthologs Pba3 and Pba4 ( Figure S5). Nevertheless, our model predicts that the complementarity at the interaction interfaces between the PAC3-PAC4 heterodimer and the proteasomal α4-α5-α6 subunits can be conserved in the yeast counterparts with a few exceptions. Perhaps the best example is the replacement of electrostatic interactions between Glu72 of PAC3 and His99 of α5 by non-polar contacts between Ala105 of Pba3 and Gln114 of α5. Therefore, despite the low sequence similarity, the overall interaction modes of the matchmaking chaperones with the proteasomal subunits appear to be conserved between humans and yeast. It is plausible that the conformational flexibility of the mobile 51-61 loop of PAC3, which carries the α6-contacting residues, contributes to the interaction adjustability.
In summary, we produced structural insights into the functional mechanisms of the PAC3-PAC4 heterodimer as a molecular matchmaker underpinning the α4-α5-α6 subcomplex during α-ring formation. These findings offer potential new approaches to the design of inhibitors against the protein-protein interactions involved in proteasome biogenesis. Although the overall structures of human PAC3 and PAC4 are similar to those of yeast Pba3 and Pba4 (RMSD = 1.9-2.1Å and 1.9-2.2 Å) respectively, their amino acid sequence similarities are low (PAC3 versus Pba3 11.0%; PAC4 versus Pba4 14.6%). The α-subunit contacting residues of human PAC3 and PAC4, as predicted by the model, are not well-conserved in the yeast orthologs Pba3 and Pba4 ( Figure S5). Nevertheless, our model predicts that the complementarity at the interaction interfaces between the PAC3-PAC4 heterodimer and the proteasomal α4-α5-α6 subunits can be conserved in the yeast counterparts with a few exceptions. Perhaps the best example is the replacement of electrostatic interactions between Glu72 of PAC3 and His99 of α5 by non-polar contacts between Ala105 of Pba3 and Gln114 of α5. Therefore, despite the low sequence similarity, the overall interaction modes of the matchmaking chaperones with the proteasomal subunits appear to be conserved between humans and yeast. It is plausible that the conformational flexibility of the mobile 51-61 loop of PAC3, which carries the α6-contacting residues, contributes to the interaction adjustability.
In summary, we produced structural insights into the functional mechanisms of the PAC3-PAC4 heterodimer as a molecular matchmaker underpinning the α4-α5-α6 subcomplex during α-ring formation. These findings offer potential new approaches to the design of inhibitors against the protein-protein interactions involved in proteasome biogenesis.

Sample Preparation
Human proteasome α6 short isoform and α7 subunits were produced and purified as previously described [22,23]. Genes encoding the proteasome α1 and α4 subunits were subcloned into NdeI and SalI sites in pET28b, and the α2 gene was inserted into the pRSFDuet-1 vector using NdeI and XhoI restriction enzyme sites (Merck Millipore, Burlington, MA, USA). As for α4, 3xFLAG sequence (DYKDHDGDYKDHDIDYKDDDDK) was added at the N-terminus. The α3 and α5 genes were subcloned into the BamHI and XhoI or SalI sites of modified pCold-I and pCold-GST vectors (TaKaRa Bio Inc., Kusatsu, Japan), respectively, in which a factor Xa cleavage site was replaced with that of TEV protease. The PAC3 and PAC4 genes were subcloned into NdeI and XhoI sites in pET28b, in which the C-terminus of PAC4 was connected to the N-terminus of PAC3 through a (GGGS) 4 liner. Standard polymerase chain reaction method was used to generate a V77S/K80A PAC3 mutant. Escherichia coli BL21-CodonPlus (DE3)-RIL (Agilent Technologies, Santa Clara, CA, USA) was used for all recombinant protein expression.
For the expression of recombinant proteins, the E. coli cells were grown in LB medium containing kanamycin or ampicillin. Briefly, the recombinant proteins were purified from the soluble fractions, except for α2, which was purified from the inclusion bodies and refolded using standard dilution methods.
Purification of these recombinant proteins was performed using affinity chromatography with Anti-FLAG M2 Affinity gel (Sigma-Aldrich, St. Louis, MO, USA), Ni + -charged Chelating Sepharose, or Glutathione Sepharose 4B, anion-exchange chromatography with RESOURCE Q resin, and size exclusion chromatography with Superdex 75 pg or 200 pg resins (GE Healthcare, Chicago, IL, USA). For NMR analyses, the PAC3 homodimer was expressed in E. coli cells which were grown in M9 minimal medium containing [ 13 C]glucose (2.0 g/L) and/or [ 15 N]NH 4 Cl (1.0 g/L), and purified using a previously-described protocol [12].

Crystallization, X-ray Data Collection, and Structure Determination
For crystallization, purified non-tagged PAC3 homodimer was produced at a concentration of 8.0 mg/mL in 20 mM Tris-HCl (pH 7.5) and 150 mM NaCl. Crystals were obtained in a buffer containing 30% PEG2000 monomethyl ether and 0.1 M potassium thiocyanate with incubation at 20 • C for three to four days. Crystals were transferred into the reservoir solution and flash-cooled in liquid nitrogen. Diffraction intensities were integrated using XDS [24] and data scaling was carried out using AIMLESS [25]. The crystals of PAC3 belonged to space group P3 1 21 and diffracted up to a resolution of 0.96 Å.
The trigonal structure of PAC3 was solved by the molecular replacement method using MOLREP [26], using the previously-reported tetragonal structure (PDB code 2Z5E) [16] as a search model. Automated model building and manual model fitting to electron density maps were performed using ARP/wARP [27] and COOT [28], respectively. Model refinement was carried out using REFMAC5 [29], and structure validation was conducted using MolProbity [30]. The data collection and refinement statistics of the PAC3 homodimer are summarized in Table S1. The molecular graphics were prepared using PyMOL (Schrödinger, New York, NY, USA).

Accession Numbers
The coordinates and structural factors of the crystal structure of the PAC3 homodimer have been deposited in the Protein Data Bank under accession number 6JPT. Backbone 1 H and 15 N chemical shift data of the PAC3 homodimer have been deposited in the Biological Magnetic Resonance Data Bank under accession number 27844.