PCSK9 is Expressed in Human Visceral Adipose Tissue and Regulated by Insulin and Cardiac Natriuretic Peptides
Abstract
:1. Introduction
2. Results
2.1. PCSK9 Expression in Human VAT
2.2. PCSK9 and LDLR Regulation in Human Adipocytes by Insulin
2.3. PCSK9/LDLR Modulation by Cardiac Natriuretic Peptides
2.4. The Influence of LDL from Human Plasma
2.5. LDL and ANP Effects on Human Adipocytes
3. Discussion
4. Materials and Methods
4.1. Reagents and Antibodies
4.2. Human Adipose Tissue
4.3. Adipocyte Cells Culture
4.4. Isolation of Human Plasma LDL
4.5. RNA Isolation and Gene Expression Analysis
4.6. Western Blotting
4.7. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Variables | N | Mean ± SE |
---|---|---|
Gender (male/female) | 26 | 15/11 |
Age (y) | 26 | 66.9 ± 1.4 |
BMI (kg/m2) | 26 | 25.6 ± 0.8 |
Waist (cm) | 26 | 96.9 ± 1.9 |
SBP (mmHg) | 24 | 139.1 ± 3.1 |
DBP (mmHg) | 24 | 78.9 ± 2.0 |
Triglycerides (mg/dL) | 17 | 125.4 ± 9.7 |
Total Cholesterol (mg/dL) | 17 | 176.1 ± 11.7 |
HDL Cholesterol (mg/dL) | 17 | 39.5 ± 2.7 |
LDL Cholesterol (mg/dL) | 17 | 118.2 ± 10.1 |
Non-HDL Cholesterol (mg/dL) | 17 | 139.7 ± 9.9 |
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Bordicchia, M.; Spannella, F.; Ferretti, G.; Bacchetti, T.; Vignini, A.; Di Pentima, C.; Mazzanti, L.; Sarzani, R. PCSK9 is Expressed in Human Visceral Adipose Tissue and Regulated by Insulin and Cardiac Natriuretic Peptides. Int. J. Mol. Sci. 2019, 20, 245. https://doi.org/10.3390/ijms20020245
Bordicchia M, Spannella F, Ferretti G, Bacchetti T, Vignini A, Di Pentima C, Mazzanti L, Sarzani R. PCSK9 is Expressed in Human Visceral Adipose Tissue and Regulated by Insulin and Cardiac Natriuretic Peptides. International Journal of Molecular Sciences. 2019; 20(2):245. https://doi.org/10.3390/ijms20020245
Chicago/Turabian StyleBordicchia, Marica, Francesco Spannella, Gianna Ferretti, Tiziana Bacchetti, Arianna Vignini, Chiara Di Pentima, Laura Mazzanti, and Riccardo Sarzani. 2019. "PCSK9 is Expressed in Human Visceral Adipose Tissue and Regulated by Insulin and Cardiac Natriuretic Peptides" International Journal of Molecular Sciences 20, no. 2: 245. https://doi.org/10.3390/ijms20020245