An Update on Pharmacological Potential of Boswellic Acids against Chronic Diseases

Natural compounds, in recent years, have attracted significant attention for their use in the prevention and treatment of diverse chronic diseases as they are devoid of major toxicities. Boswellic acid (BA), a series of pentacyclic triterpene molecules, is isolated from the gum resin of Boswellia serrata and Boswellia carteri. It proved to be one such agent that has exhibited efficacy against various chronic diseases like arthritis, diabetes, asthma, cancer, inflammatory bowel disease, Parkinson’s disease, Alzheimer’s, etc. The molecular targets attributed to its wide range of biological activities include transcription factors, kinases, enzymes, receptors, growth factors, etc. The present review is an attempt to demonstrate the diverse pharmacological uses of BA, along with its underlying molecular mechanism of action against different ailments. Further, this review also discusses the roadblocks associated with the pharmacokinetics and bioavailability of this promising compound and strategies to overcome those limitations for developing it as an effective drug for the clinical management of chronic diseases.


Introduction
Chronic disease can be defined as a physical or psychological state that leads to functional limitations or requires constant observation or treatment for a long period. Worldwide, chronic diseases have hampered the health and living conditions of many [1]. Many of the universally used clinical drugs (especially the biologics) these days bear the shortcomings of side effects and high treatment cost [2]. Thus, numerous natural compounds, which have identified as potent modulators of signaling and epigenetic pathways leading to cancer, are under development presently [3]. Natural products have gained considerable attention as they are plentiful sources of diverse compounds, which can function as biologically active drugs against different chronic diseases [4][5][6][7][8][9][10][11][12][13][14]. These plant-derived molecules have significantly enhanced the existing medicinal system. For example, in a developing nation like India, around 65% of the country's population gets benefitted by the use of phytomedicines that play an essential role in the health management system. satisfying. In developed nations like the USA, the sale of phytomedicines has registered a sharp incline in recent years. Around 80% of nations like the USA, the sale of phytomedicines has registered a sharp incline in recent years. Around 80% of the African population relies on the use of phytomedicines to meet their health care needs through the use of traditional medicines. According to the WHO, nearly 80% of the world's population uses phytomedicines for the management of various ailments [15][16][17][18][19][20][21][22][23][24].
For generations, numerous natural compounds extracted from different plants and showing significant pharmacological properties, have used for treating various chronic diseases. To date, about 10,000 phytochemicals comprising of tannins, flavones, triterpenoids, steroids, saponins, and alkaloids have identified, and many more are yet to discover. It is believed that the antioxidant activity of phytochemicals increases their action synergistically, as numerous reports evidenced that overproduction of oxidants (reactive oxygen species and reactive nitrogen species) causes many chronic diseases such as cardiovascular diseases (CVD), diabetes, and cancers [25][26][27][28][29][30][31].
Boswellic acid (BA) is one such phytochemical,obtained from the gum resin of the Boswellia species, that possibly aid in the treatment of different chronic diseases. Traditionally, the gum resins of Boswellia species found its applications in various adhesives, cosmetic preparations, coating materials, the incense used in cultural rites and rituals, and many more. It is one of the most essential and commonly used components in conventional Ayurvedic and Unani medicines, which have proven to be extremely effective in relieving numerous inflammatory, gastrointestinal, hormonal, and microbial diseases [32]. The conventional drug is said to have the properties of an antiinflammatory, antiseptic, expectorant, anxiolytic, antineurotic, analgesic, and tranquilizing drug [33]. Various preclinical and clinical studies have established that it exhibits substantial potential in the management of inflammatory ailments such as asthma, arthritis, cerebral edema, chronic bowel diseases, chronic pain syndrome, cancer, etc [34,35].

Potential Role of BAs in the Treatment of Chronic Diseases
As aforementioned, BA is a multitargeted compound, which enables its use against diverse diseases ( Figure 4). The prospective of BAs in managing various chronic diseases is well evidenced by a number of preclinical studies through their ability to modulate multiple mediators involved in the pathogenesis of diverse diseases (Table 1).

Potential Role of BAs in the Treatment of Chronic Diseases
As aforementioned, BA is a multitargeted compound, which enables its use against diverse diseases ( Figure 4). The prospective of BAs in managing various chronic diseases is well evidenced by a number of preclinical studies through their ability to modulate multiple mediators involved in the pathogenesis of diverse diseases (Table 1).

Arthritis
Arthritis predominantly arises due to inflammation of joints and the connective tissues surrounding them. Osteoarthritis, being the predominant of all forms, affects a wide range of the population all over the world [104]. A study on the effect of BAs in bovine serum albumin (BSA)induced arthritis reported that on oral administration, BAs (25, 50, and 100 mg/kg/day) noticeably

Asthma
Asthma is rising as a severe global health issue, which is characterized by airway hyperresponsiveness, airway inflammation, enhanced mucus production, airway epithelial wall shedding, and an increase in the IgE levels. An investigation on the anti-asthmatic potential of BA in a murine model of asthma reported suppression of allergic airway inflammation, AHR, OVA-specific IgE, and Th2 cytokines secretion were in treated groups. Furthermore, the expression of p-STAT6 and GATA3 were also suppressed in a dose-dependent manner [110]. In another in vivo study, the effect of BA was analyzed by injecting a sensitization liquid (0.15 mL aluminum hydroxide gel at 88.67 mg/mL and 0.05 mg ovalbumin) intraperitoneally in an asthma model, and it was found to minimize the symptoms by abrogating p-STAT6 followed by a reduction in GATA3 expression [111].

Atherosclerosis
Atherosclerosis occurs due to the formation of plaque inside the blood vessels leading to thickening of the arteries. An investigation on the effect of AKBA in apolipoprotein E-deficient (ApoE−/−) mice showed that it inhibited NF-κB, a vital element for the development and prognosis of various inflammatory diseases. Thus, this finding suggests that the plant resins from the Boswellia family can provide a substitute for conventional treatment strategies for chronic inflammatory diseases like atherosclerosis [86].

Cancer
Cancer is one of the most fatal diseases of mankind, with extremely high incidence and mortality rate. In the year 2012, it was estimated that about 14.1 million people suffered from the disease and 8.2 million people succumbed to death, whereas in the year 2018, the number of deaths increased to 9.8 million worldwide [162][163][164][165][166]. Notably, the majority of the existing drugs exert severe side effects and are mostly ineffective due to the development of chemoresistance [23, [167][168][169]. This has led to shifting of attention towards natural products such as butein, emodin, curcumin, epigallocatechin gallate (EGCG), celastrol, honokiol, resveratrol, etc. which have shown high potential against various types of cancer [23, [170][171][172][173]. Moreover, different studies have shown the efficacy of BA in the prevention and treatment of breast, bladder, cervical, prostate, colorectal, head and neck, liver, lung, and pancreatic cancers, etc. [36].

Bladder Cancer
Nearly, 430,000 people are diagnosed with bladder cancer annually, and 165,000 people die every year all across the globe [174]. To evaluate the anti-cancer effect of frankincense oil, (main component of which is BA), an in vitro study on J82 (human bladder cancer) and UROtsa cells (immortalized normal bladder urothelial cells) was performed. Treatment with frankincense oil exerted cytotoxic effects on the J82 cell line but had minimal effect on UROtsa cells. Thus, frankincense oil was found to differentiate between cancerous cells and normal cells and caused the suppression of tumor cell viability [113].

Brain Cancer
Approximately 256,213 individuals, which includes 116,605 females and 139,608 males, in the year 2012, were diagnosed with a primary malignant brain tumor, globally [175]. Glaser et al., in 1999, observed that at low micromolar concentrations, BAs showed cytotoxicity against malignant glioma cells [122]. Further, the pure extract of the gum resin of B.serrata and various analogues of BA including AKBA, BBA, and cyano enone of methyl boswellates (CEMB) have shown cytostatic and apoptosis-inducing activity against glioma cells [124,125].. Studies by Park et al., on meningioma cells also suggested that the cytotoxic action of AKBA might, at least in part, be mediated by Erk signal transduction pathway inhibition. Furthermore, in vivo studies on an immunocompromised mice model (C6 glioma tumor xenograft) reported that intratumor administration of CEMB significantly inhibited the tumor growth, signifying the potent antitumor effect of CEMB [123].

Cervical Cancer
One of the most common reasons for female malignancy in the world is cervical cancer, causing approximately 265,700 deaths annually [176]. In cervical cancer, treatment with 3-α-propionyloxy-β-BA (POBA) caused PARP cleavage, which consequently led to a cell cycle arrest, DNA fragmentation, and loss of mitochondrial membrane potential in SiHa cells [115].

Colon Cancer
The apoptotic and antiproliferative effects of the analogues of BA, such as BBA, KBA, and AKBA in colon cancer cells, were analyzed. It was observed that AKBA could induce apoptosis through caspase activation and the p21-dependent pathway [87,114]. Moreover, studies on APC (Min/+) mice have shown the chemopreventive action of AKBA against intestinal adenomatous polyposis by inhibiting Wnt/β-catenin and NF-κB/Cox-2 signaling pathways [91]. In vitro studies on human colon cancer cells further showed that the potent anticancer effects of BA might be mediated via induction of apoptosis and cell cycle arrest, as well as abrogation ofPI3K/Akt signaling pathway [42].
Also, AKBA affected the growth of colorectal cancer cells through genetic (Ki-67 and CD31) and epigenetic modulations (demethylation and miRNA regulation) [34, 116,117]. Furthermore, AKBA, in combination with curcumin, showed antitumorigenic effects in vitro and in vivo by regulating specific cancer-related miRNAs such as miR-34a and miR-27a in colorectal cancer cells [177].

Leukemia
The antitumor activity of BA and its analogues, such as BBA, KBA, AKBA, and PKBA, were studied in different leukemic cell lines such as HL-60, K562, MOLT-4, THP-1, CCRF-CEM, ML-1, NB4, SKNO-1, and U937 cells. Results showed that the treatment with BA exerted cytostatic and cytotoxic effects through the induction of apoptosis. Upon examining the molecular mechanisms involved, it was found that the treatment resulted in the attenuation of topoisomerases I and II, the release of cytochrome c, the loss of mitochondrial membrane potential, activation of caspases, and cleavage of PARP. It was also reported that the treatment led to the decreased expression of MMP-1, MMP-2, and MMP-9 mRNAs; along with the secretions of TNF-α and IL-1β; reduced the phosphorylation of ERK1/2, p38 MAPKs; and disrupted PI3K/AKT/Hsp-90 cascade [95,97,[126][127][128].

Liver Cancer
Around 782,500 new cases and 745,500 cancer-related deaths have occurred due to liver cancer or hepatocellular cancer in the year 2012 [66,[178][179][180][181][182][183][184][185]. When the effects of KBA and AKBA were evaluated, they were found to inhibit proliferation and induce apoptosis through the caspase-8-dependent pathway in liver cancer cells [130]. Also, BSE, when administered as monotherapy and in combination therapy with DOX, caused an augmentation in caspase-3 activity, TNF-α, and IL-6 levels, thus showing growth-modulatory and apoptotic actions in hepatocellular carcinoma cells [186].

Lung Cancer
An in vitro study on H446 cells was performed to explore the antitumor potential of 11-carbonyl-BBA. It was found to activate JNK signaling pathway, cause the cleavage of PARP, and downregulate survivin protein expression, thus showing inhibitory effects on lung cancer cells [131]. Moreover, a study focusing on the potential of POBA showed that POBA initiated PARP cleavage on HOP-62 lung cancer cells. As a consequence of the treatment, induction of apoptosis, as well as cell cycle arrest occurred in lung cancer cells [115].
3.4.9. Prostate Cancer GLOBOCAN 2012 reported that prostate cancer accounts for nearly 1.1 million new cases all across the world [162,[187][188][189][190][191][192]. AKBA was shown to elicit cell death and reduce cell proliferation in PC-3 prostate cancer cell lines by abrogation of the activated NF-κB signaling pathway via interception of IκB kinase activity and activation of caspase-3 [88,113]. In LnCaP and PC-3 prostate cancer cells, AKBA showed apoptotic effects driven by the death receptor 5-mediated pathway. Besides that, caspase-3 and caspase-8 activation, as well as PARP cleavage induction, were evidenced [99]. In another study, AKBA was found responsible for the suppression of VEGFR2-mediated angiogenesis in prostate cancer [101]. Studies by Liu et al., showed that AKBA suppressed docetaxel-resistant prostate cancer cells via blockage of STAT3 and Akt signaling pathways [138]. A semi-synthetic triterpenoid derivative, 3-cinnamoyl-11-keto-beta-BA (C-KβBA), demonstrated specific antiproliferative and proapoptotic effects in cancer cell lines such as PC-3, LnCaP, and DU-145, as well as in PC-3 prostate cancer xenografts, by downregulating the activation of p70 ribosomal S6 kinase [122].

Pancreatic Cancer
Pancreatic cancer is the seventh most leading cause of cancer deaths in the world, and the rate of incidence is parallel to the rate of mortality due to pancreatic cancer [193]. To evaluate the role of AKBA, different in vitro studies on pancreatic cancer cell lines, such as AsPC-1 and PANC-28, and in vivo studies were performed. AKBA was found to inhibitcell growth and downregulate the expressions of Ki-67, CD31, Cox-2, MMP-9, CXCR4, and VEGF in the tumor tissues [90]. Recently, combination of the anti-diabetic drug metformin and BA nanoparticles showed synergism in inhibiting the growth of pancreatic cancer cells [194].

Melanoma
Mainly the population of the world bearing white skin is prone to melanoma, and it is considered as a serious global concern [195]. The effect of an isomeric compound, BC-4, containing both αand β-BA acetate was studied via in vitro study. It was observed that BC-4 was responsible for the induction of B16F10 cells differentiation, blockage of the cell population in the G1 phase of the cell cycle, attenuation of topoisomerase II activity as well as the migratory potential of B16F10 cells when administered at a concentration of 25µM for 48h. Further, in fibrosarcoma cells, HT-1080 apoptosis was induced, and MMP secretion was reduced after treatment with BA [96].

Renal Intestinal Fibrosis
The role of AKBA in renal-intestinal fibrosis was studied both in vitro and in vivo using hypoxia-induced HK-2 cells and C57BL/6 mice, respectively via unilateral ureteral obstruction (UUO). The findings showed that AKBA exhibited a renoprotective effect via modulation of the Klotho/TGF-β/Smad signaling pathways. Hence, AKBA can be employed effectively for the treatment of renal-intestinal fibrosis [159].

Inflammatory Bowel Diseases (IBDs)
IBDs can be defined as idiopathic chronic relapsing malfunctions of the gastrointestinal tract (GIT) with an unknown origin, is characterized by the heterogeneity and multifactorial nature of their pathogenesis [196]. Ulcerative colitis affects the colon, where leukotrienes play a significant role. A study on effects of the BSE in patients with ulcerative colitis illustrated that administration of BSE for six weeks improved the stool properties, histopathology, and blood parameters, including Hb, serum iron, calcium, phosphorus, proteins, total leukocytes, and eosinophils [197]. Further, in an attempt to study the effect of AKBA on experimental ileitis, it was observed that treatment with AKBA caused a significant decrease in rolling (up to 90%) and adherent (up to 98%) leukocytes. Also, high doses of Boswellia extract, as well as AKBA, significantly reduced tissue injury scores [198]. Moreover, in an investigation on the effects of BSE in mouse models of chemically induced colitis, it was found that BA was incapable of ameliorating the symptoms of colitis and it exerted hepatotoxicity at higher doses [199]. Contrary to this report, another study demonstrated the anti-inflammatory effect of the semisynthetic form of AKBA and showed that P-selectin-regulated recruitment of inflammatory cells may be a major site of action for this novel anti-inflammatory agent in dextran sodium sulfate (DSS)-induced experimental murine colitis [200].

Diabetes
Diabetes is becoming the leading causes of death worldwide. It is classified into two types-Type1 diabetes (T1D) and Type 2diabetes(T2D).T1D is an autoimmune disorder whereas T2D is a metabolic disorder [201]. A study on alloxan-induced diabetic rats reported significant hypoglycemic effects on the continued use of the aqueous extract of leaves and roots of Boswellia glabra. Moreover, a decrease in the serum glucose level, cholesterol, triglyceride, urea and creatinine levels, and enzyme activities (alkaline phosphatase and glucose-6-phosphatase) was observed after treatment [144]. Also, it was observed that the administration of BSE can cause a significant decrease in blood glucose level along with HbA1c, cholesterol, LDL, and fructosamine [145,146,202]. Likewise, the isolated compounds from the plant, such as KBA and AKBA prevented the occurrence of autoimmune reactions, insulitis, and reduced hyperglycemia in multiple low-dose streptozotocin (MLD-STZ)-induced diabetes models [147].

Central Nervous System Disorders
BAs may also have tremendous potential in the treatment of central nervous system disorders such as Parkinson's, Alzheimer's disease, and cognitive impairment. Treatment with BAs has shown reduced inflammatory markers, improved general motor performance, nigral tyrosine hydroxylase immunostaining, and increased striatal dopamine levels in Parkinsonian rats [134]. The effects of α-BA were investigated in primary fetal human astrocytes under a stress paradigm as a probable model for Alzheimer's disease. The results showed that α-BA could be considered as an effective remedy for prevention and lessening the progression of Alzheimer's hallmarks in astrocytes; though, further preclinical findings are critical [109]. In a neuroinflammatory model of mice, AKBA showed antiapoptotic and anti-amyloidogenic effects via modulation of miRNA-155 [161]. Moreover, BA exhibited a neuroprotective role in Wistar rat models of cognitive impairment [203]. In another model of cognitive dysfunction, combination treatment with AKBA and celecoxib exhibited anti-inflammatory, antiglutamatergic, and antiamyloidogenic properties, leading to better prognosis of the disease [119].

Ischemia-Reperfusion Injury (IRI)
IRI is a physiopathological condition involving numerous metabolic processes which finally leads to cell apoptosis and ultimately tissue necrosis [204]. The protective effect of KBA against myocardial IRI in rats was observed. Three dose levels of KBA exerted dose-dependent cardioprotective effects, as manifested by a dose-dependent drop in serum lactate dehydrogenase and infarct size [149]. In ischemic brain injury also, AKBA was responsible for neuroprotection that involved the Nrf2/HO-1 defense pathway. It was found that the administration of AKBA increased Nrf2 and HO-1 expression, and a similar observation was also made for the compound KBA against cerebral ischemia-reperfusion injury [150,151].

Psoriasis
The gum resin of B. serrata has been also found effective in curing diverse skin problems such as psoriasis. A study was conducted to evaluate the effect of AKBA using murine bone marrow-derived dendritic cells (BMDCs) and a psoriasis-like mouse model, respectively. The results confirmed the anti-inflammatory effects of AKBA on psoriasis via modulation of IRF and TLR7/8 signaling pathways [140].

Other Diseases
Apart from the above mentioned diseases, a few reports on other diseases are also available where positive effects of BAs have been observed. In a study on guinea pigs with experimental autoimmune encephalomyelitis, BAs were found to reduce the clinical symptoms of the disease [205]. In an attempt to assess the antiulcer properties of BA, it was found to inhibit the ulcer formation in different experimental models. It was suggested that the protective action comes from enhanced gastric mucosal resistance, cytoprotective prostaglandins synthesis, and leukotriene synthesis inhibition [154]. A study on the gastroprotective role of α-BA was performed in ethanol-induced gastric injury in rats. The findings demonstrated that α-BA decreased ethanol administration related injuries, gastric juice acidity, and the development of MDA, and improved CAT activity along with SOD activity and the level of NO and PGE-2 [153]. In the case of myocardial injury, AKBA in combination with HSYA showed cardioprotective effects via modulation of the PGC-1α/Nrf2 pathway [132]. In another study, the efficacy ofBA against acetaminophen (APAP)-induced hepatotoxicity in Balb/c mice was determined. It was observed that BA pre-intake reduced APAP-induced production of inflammatory cytokines and chemokines. Further, it affected the expression of NF-κB p65 and p-JNK, TLR-3, TLR-4, and MyD88 [155,206].
Recently, BSE in combination therapy with curcumin was found to inhibit chikungunya and vesicular stomatitis virus infections in vitro. The combination therapy was able to block the entry of CHIKV Env-pseudotyped lentiviral vectors, and they suppressed CHIKV infection in vitro. Furthermore, vesicular stomatitis virus vector particles and viral infections were also reduced, thereby demonstrating its broad antiviral activity [143]. An in vitro study was performed to explore the ethnomedicinal use of BSE, and it was proved that BSE, as well as BA, efficiently inhibited wild-type and a clinical isolate of HSV-1 via alteration of NF-κB and p38 MAPK pathways [157]. Furthermore, in another study on a mouse model of LPS-induced neuroinflammation, AKBA played a significant role in counteracting the symptoms via modulation of miRNA-155 [161]. Treatment of mouse models of Ehrlich tumor and Ehrlich ascites carcinoma with BA have demonstrated the antitumor property of the compound by interfering with the IL-6-STAT-3 signal transduction pathway. APOBA treatment on Ehrlich ascites carcinoma (EAC) cells and sarcoma 180 (S-180) cells also witnessed tumor growth inhibition [115,120,121]. In the pulmonary arterial hypertensive rat model, α-BA administration showed protective effects by downregulating the expression of JNK and protein kinase 1 under hypoxic conditions [142]. IN0523 (Urs-12-ene-3α,24β-diol), a derivative of BA, was found to show protective effects in response to cisplatin-induced urogenital toxicity by inhibiting the imbalance of oxidative stress/redox state and by enhancing the efflux mechanisms [160].

Boswellic Acid Implicated in Different Phases of Human Clinical Trials
As aforementioned, not only the preclinical studies but also the studies carried out in the clinical settings well evinced the high potential of BA against diverse chronic diseases( Table 2). In a double-blind, placebo-controlled human trial, oral administration of Boswellin, a formulation containing AKBA and BBA, was found to exert anti-inflammatory/antiarthritic effects in osteoarthritis patients [207]. Further, a novel BSE containing 30% AKBA and known as 5-Loxin led to improved physical functioning and decreased pain in patients with osteoarthritis, plausibly via regulation of inflammatory responses by decreasing pro-inflammatory modulators and enzymatic degradation of cartilage, without exerting any toxic effect [208]. Also, a comparative, randomized, double-blind, placebo-controlled study, which examined the efficacy and safety of curcumin in combination with BA, exhibited favorable responses in patients with osteoarthritis [209]. Additionally, another combination of BA with methylsulfonylmethane (MSM) also displayed satisfactory outcomes in the treatment of knee arthritis [210]. However, Notarnicolaet al. showed this combination not to exert much efficacy in the case of gonarthrosis [211]. Additionally, a lecithin-based delivery form of B.serrata, named as Casperome ® , was reported to improve the signs and symptoms of patients with irritable bowel syndrome in a highly safe and effective fashion [212]. A double-blind placebo-controlled study as well witnessed that administration of BSE containing BAs in major proportions led to significant improvement in patients suffering from bronchial asthma. The symptoms such as dyspnoea, rhonchi, number of attacks, increase in FEV subset1, FVC, and PEFR, in addition to a decline in the eosinophilic count and ESR, were evidenced [213]. Further, treatment with BSEdecreased cerebral edema in patients irradiated for brain tumors significantly, as evinced by a prospective, randomized, placebo-controlled, double-blind pilot trial [214]. Besides, a clinical trial conducted by Gerhardt and group to compare the efficacy and safety of H15, a BSE with mesalazine for the treatment of active Crohn's disease, H15 was found to exert better effect concerning a benefit-risk evaluation [215]. Also, In a double-blind study, it was witnessed that a novel BA formulation (consisting of Bosexil( ® ), INCI (International Nomenclature of Cosmetic Ingredients): lecithin, an extract of B. serrata resin) was a favorable candidate for therapy of patients suffering from erythematous eczema and psoriasis [216]. Furthermore, the topical application of a cream containing 0.5% BA presented a well-tolerated and safe treatment approach for photoaged skin [217,218]. Thus, these studies clearly indicate BA to be safe, well-tolerated, and effective, and thus implies its high therapeutic potential against a wide array of human chronic diseases. Abbreviations: a = BA in combination with curcumin; b = 5-Loxin, a novel Boswellia serrata extract enriched with 30% AKBA; c = BA in combination with methylsulfonylmethane; d = Boswellia serrata extract H15; A = Phase I; B = Phase II; C = NA, HDL = high density lipoprotein; ESR= erythrocyte sedimentation rate.*= All the studies listed above are completed.

Pharmacokinetic Properties of Boswellic Acids
BAs are the chief bioactive element of frankincense, and various studies have established their bioactivities. However, to develop it as a successful candidate drug, its pharmacokinetic properties must be considered accurately. In this regard, the studies conducted have exhibited poor pharmacological performance. Both KBA and AKBA are extremely lipophilic drugs, which result in reduced absorption through the GIT, but they also exhibit high retention time. Preliminary pharmacokinetic studies have shown minimal concentrations of both AKBA and KBA in human plasma after administration of BSE [41, [219][220][221][222][223]. Moreover, the incidence of AKBA in plasma is uncertain due to its deacetylation to KBA in vivo [222]. Contrary to this report, another study on the metabolism of BAs indicated that AKBA is not deacetylated to KBA. Furthermore, it was demonstrated that unlike AKBA, KBA experiences extensive phase I metabolism in rat and human liver microsomes, as well as in hepatocytes. The metabolic profiles of KBA in rat plasma and liver were found to be similar in both in vitro and in vivo study whereas no metabolites of AKBA could be recognized. This indicates that the administration step should be further implemented to increase the bioavailability of AKBA [224]. Another study reported that the foremost permeability-associated barriers that compromised oral bioavailability of KBA include its gastrointestinal volatility, CYP3A4, mediated intestinal metabolism, accumulation within the enterocytes, and saturable kinetics [225]. In another study, the metabolic stability, permeability and brain availability of six major BAs, i.e., KBA, AKBA, βBA, 3-acetyl-β-BA (AβBA), αBA, and 3-acetyl-α-BA (AαBA) was evaluated. The four BAs lacking the 11-keto moiety showed reasonable permeability. In contrast to AαBA and AβBA, βBA, and αBA were effectively metabolized and also the availability of all six major BAs was confirmed in rat brain eight hours after oral administration of 240 mg/kg BSE to rats [226].
One more important pharmacokinetic parameter to be kept in mind while experimenting with a drug is its elimination from the body. A study reported that the elimination half-life of BA is about six hours. This suggests that oral administration of the medication is required after every six hours. The study also reported that a stable state of the drug would be achieved in the plasma after around thirty hours [222].

Improvement in the Bioavailability of Boswellic Acids
In regard of the relatively low plasma and brain levels of BAs, and as a consequence of their inability to inhibit 5-LOX in whole blood, the abrogation of LTB4 synthesis in vivo by frankincense extracts remains unclear. For exploiting the potential pharmacological properties of different BAs, several approaches have been used to enhance its bioavailability [228]. Some researchers have also tried to enhance the bioavailability of BAs by administering it with a standardized meal [229]. Also, an improvement in their uptake was observed when it was administered with anionic drugs [230]. Further, different methods such as lecithin delivery form (PhytosomeR); nanoparticle delivery systems like liposomes, emulsions, solid lipid nanoparticles, nanostructured lipid carriers, micelles, and poly (lactic-co-glycolic acid) nanoparticles; and synthetic derivatization of BA have been adapted for overcoming these limitations [231][232][233]. Formulation of BA with lecithin was found to improve absorption and tissue penetration of BA in a single-dose, randomized, open-label study [234].

Conclusions
BAs, the pentacyclic triterpenic acids comprising of α-,β-,γ-BA,acetyl-β-BA, KBA, AKBA, and so on, have exhibited diverse pharmacological activities against various chronic diseases, as evidenced through the multiple preclinical studies and various clinical trials. They can target several key players involved in the pathogenesis of these diseases. It was observed that different important molecular targets are affected by BA treatment, such as LO, MAPK, NF-κB, TNF-α, Erk-1/2, etc., which plays an imperative role in the development of various chronic diseases. Yet, concerns regarding the pharmacokinetic properties have had major dampening effects in the path of development of this compound as an effective drug. Nevertheless, many investigations have been initiated in this matter to triumph over the limitations, but the pace is quite slow, and an ample amount of attention is needed.