TCO, a Putative Transcriptional Regulator in Arabidopsis, Is a Target of the Protein Kinase CK2

Weinman, Laina M.; Running, Katherine L. D.; Carey, Nicholas S.; Stevenson, Erica J.; Swaney, Danielle L.; Chow, Brenda Y.; Krogan, Nevan J.; Krogan, Naden T.

doi:10.3390/ijms20010099

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TCO, a Putative Transcriptional Regulator in Arabidopsis, Is a Target of the Protein Kinase CK2

Laina M. Weinman

¹,

Katherine L. D. Running

¹,

²,

^2,3,

¹,

^2,3 and

^1,*

Department of Biology, American University, 4400 Massachusetts Avenue NW, Washington, DC 20016, USA

Gladstone Institute of Data Science and Biotechnology, J. David Gladstone Institutes, San Francisco, CA 94158, USA

Quantitative Biosciences Institute (QBI), University of California, San Francisco, CA 94158, USA

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2019, 20(1), 99; https://doi.org/10.3390/ijms20010099

Received: 7 November 2018 / Revised: 26 December 2018 / Accepted: 26 December 2018 / Published: 28 December 2018

(This article belongs to the Special Issue Molecular Research in Arabidopsis)

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Abstract

As multicellular organisms grow, spatial and temporal patterns of gene expression are strictly regulated to ensure that developmental programs are invoked at appropriate stages. In this work, we describe a putative transcriptional regulator in Arabidopsis, TACO LEAF (TCO), whose overexpression results in the ectopic activation of reproductive genes during vegetative growth. Isolated as an activation-tagged allele, tco-1D displays gene misexpression and phenotypic abnormalities, such as curled leaves and early flowering, characteristic of chromatin regulatory mutants. A role for TCO in this mode of transcriptional regulation is further supported by the subnuclear accumulation patterns of TCO protein and genetic interactions between tco-1D and chromatin modifier mutants. The endogenous expression pattern of TCO and gene misregulation in tco loss-of-function mutants indicate that this factor is involved in seed development. We also demonstrate that specific serine residues of TCO protein are targeted by the ubiquitous kinase CK2. Collectively, these results identify TCO as a novel regulator of gene expression whose activity is likely influenced by phosphorylation, as is the case with many chromatin regulators. View Full-Text

Keywords: Arabidopsis thaliana; chromatin regulation; CK2; development; phosphorylation; protein kinase; transcriptional regulation

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Weinman, L.M.; Running, K.L.D.; Carey, N.S.; Stevenson, E.J.; Swaney, D.L.; Chow, B.Y.; Krogan, N.J.; Krogan, N.T. TCO, a Putative Transcriptional Regulator in Arabidopsis, Is a Target of the Protein Kinase CK2. Int. J. Mol. Sci. 2019, 20, 99. https://doi.org/10.3390/ijms20010099

AMA Style

Weinman LM, Running KLD, Carey NS, Stevenson EJ, Swaney DL, Chow BY, Krogan NJ, Krogan NT. TCO, a Putative Transcriptional Regulator in Arabidopsis, Is a Target of the Protein Kinase CK2. International Journal of Molecular Sciences. 2019; 20(1):99. https://doi.org/10.3390/ijms20010099

Chicago/Turabian Style

Weinman, Laina M., Katherine L.D. Running, Nicholas S. Carey, Erica J. Stevenson, Danielle L. Swaney, Brenda Y. Chow, Nevan J. Krogan, and Naden T. Krogan. 2019. "TCO, a Putative Transcriptional Regulator in Arabidopsis, Is a Target of the Protein Kinase CK2" International Journal of Molecular Sciences 20, no. 1: 99. https://doi.org/10.3390/ijms20010099

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TCO, a Putative Transcriptional Regulator in Arabidopsis, Is a Target of the Protein Kinase CK2

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