Proteomic studies reveal Disrupted in Schizophrenia 1 as a key regulator unifying neurodevelopment and synaptic function

A balanced chromosomal translocation disrupting DISC1 (Disrupted in Schizophrenia 1) gene has been linked to psychiatric diseases, such as major depression, bipolar disorder and schizophrenia. Since the discovery of this translocation, many studies have focused on understating the role of the truncated isoform of DISC1, hypothesizing that the gain of function of this protein could be behind the neurobiology of mental conditions, but not so many studies have focused in the mechanisms impaired due to its loss of function. For that reason, we performed an analysis on the cellular proteome of primary neurons in which DISC1 was knocked down with the goal of identifying relevant pathways directly affected by DISC1 loss of function. Using an unbiased proteomic approach, we found that the expression of 31 proteins related to neurodevelopment (e.g. CRMP-2, stathmin) and synaptic function (e.g. MUNC-18, NCS-1) is regulated by DISC1 in primary mouse neurons. Hence, this study reinforces the idea that DISC1 is a unifying regulator of both neurodevelopment and synaptic function, thereby providing a link between these two key anatomical and cellular circuitries.


Introduction
Disrupted in Schizophrenia 1 (DISC1) gene was found mutated when studying a chromosomal translocation t(1;11)(q42.1;q14.3) in a Scottish family, this translocation correlated with cases of schizophrenia, bipolar disorder and major depression [1,2].Further studies also found that the truncation of this gene in an American family segregated with cases of schizophrenia [3].
In order to acquire a deeper understanding of the mechanisms of action of DISC1, several proteomic analysis have been conducted to specifically address the role of the truncated isoform of DISC1 on the cellular proteome of neural cells [20,21].In this study, we decided to specifically address the role of DISC1 loss of function, for that we carried out an unbiased proteomic analysis in DISC1silenced neurons.
We report that DISC1 regulates the expression of many relevant proteins related to neurodevelopment and synaptic function, reinforcing the idea that DISC1 is a key molecular link bridging neurodevelopmental functions with the regulation of synaptic formation and neurosignaling processes.

Proteomic analysis
Cell extracts from control and DISC1 knockdown murine primary neurons (Fig S1 ) were subjected to proteomic analysis.Four bidimensional gels for the silenced condition versus four of the control condition were analyzed.3474 identical spots per gel were detected (Figure S2) and 68 of them were found differentially expressed with a fold change ≥2 and p value <0.05.These spots corresponded to 48 unique proteins (Table 1) identified using mass spectrometry.The functions of these proteins were mainly related to neurodevelopmental processes or synaptic function (Table 1, Figure S3).Particularly, 19 of them were related to neurodevelopmental processes (Table 1) and other 19 unique proteins were related to synaptic function (Table 1).Of note, 7 of these proteins have shared functions (Table 1, Figure S3).Therefore, these results suggest that DISC1 plays an important role linking these two processes.
Remarkably, some of the identified proteins have been previously described as DISC1 binding partners, it is the case of 14-3-3 proteins [12] and LIS1 [28], while CRMP-2 has been identified as a possible DISC1 interactor [16].However, to the best of our knowledge, this is the first time that DISC1 has been found to also regulate their expression.As well, we could identify some of the proteins as substrates of similar enzymes; this is the case of stathmin, CRMP-2, and MAP1B.These proteins are known to be phosphorylated by GSK3β to exert their functions.Table 1.Proteins involved in neurodevelopment or synaptic function identified through proteomic analysis of primary neurons 1 .Microtubule-associated protein (MAP1B) 14-3-3 protein epsilon (14-3-3ε) 14-3-3 protein zeta/delta (14-3-3θ/Δ) 6.713x10 -5 , 1.854x10 -4

Ingenuity Pathway
In order to identify common molecular pathways regulated by DISC1 in our sample set we used the Ingenuity Pathways Analysis (IPA) software.The 5 top canonical pathways involved in our analysis are represented in Table 2.It is interesting that CRMP (collapsin response mediator protein) family was highlighted in the analysis as part of the Semaphorin signaling in neurons, since this signaling cascade is known to play an important role in neuronal differentiation and axonal growth [29,30].Previous studies also concluded that the overexpression of the truncated isoform of DISC1 leads to dysregulation of Semaphorin signaling [20].This could be a corroborative evidence for the fact that DISC1 expression has to be tightly and precisely regulated in a small window and that both, above and below that window you have dysregulation of similar signaling pathways.

DISC1 regulates the expression of neurodevelopmental related proteins
Considering the results obtained by IPA analysis we focused on the collapsin response mediator proteins (CRMPs) to perform our validations.These proteins constitute a family of five homologous cytosolic proteins (CRMP-1-5) involved in microtubule regulation.All of them are phosphorylated and highly expressed in the developing and adult nervous system where they play important roles in neuronal development and maturation [31].Six spots corresponding to CRMP-5, CRMP-3, CRMP-2 and CRMP-1 were differentially expressed in silenced vs. control cells (Table 1) in our study; in all cases the proteins were upregulated in DISC1 silenced cells.
Particularly, CRMP-2 has been described as a candidate gene for susceptibility to schizophrenia [32] and was found upregulated in a proteomic study performed with brain samples from patients with bipolar disorder, schizophrenia and major depression [33].We showed differential expression of multiple CRMP2 isoforms upon DISC1 silencing (Figure 1) in primary neurons.The existence of different isoforms of CRMP2 has been highlighted in several studies [35,36].Here, CRMP2 was detected as three isoforms (labelled 1 to 3).Isoforms 1 and 2, most likely corresponding to CRMP2A and CRMP2B [35] were found to be downregulated in DISC1-silenced cells, while isoform 3 was upregulated.A similar pattern was observed using antibodies that recognize CRMP-2 phosphorylated at Thr-514 (Figure 1).Therefore, isoform 3 most likely corresponds to the spot that was differentially expressed in our proteomic analysis.
Some studies described this isoform as a calpain-associated degradation product [37,38], while others highlight its role in neurite outgrowth inhibition (39).If this is the case, it suggests that DISC1 silencing leads to increased expression of CRMP-2 and, as a result, inhibition of neurite outgrowth.Of note, Septin-5, a protein that directly interacts with CRMP-2, was also found differentially expressed in our study (Table 1).
As well, among the spots differentially expressed, Stathmin was found downregulated in DISC1 silenced cells (Table 1) and we confirmed this result using a 2-DE western blot (Figure 2).Interestingly, this protein is involved in the control of microtubule dynamics.In particular, stathmins interact with tubulin, inhibiting its polymerization capacity and thus disrupting microtubule assembly, which regulates axonal and neurite outgrowth [40].GSK3β modulates this interaction by phosphorylating Stathmin at Ser-60, Ser-31, or Ser-38 residues [41].Therefore, we decided to test the phosphorylated status of Stathmin in our system using a commercially available antibody for pSer-38 (Figure 2a).No differences were found with this antibody, concluding Ser-38 is not the relevant phosphorylation site affected under the study conditions.

DISC1 regulates the expression of synaptic function related proteins
We also consider of great relevance that endocytosis was highlighted under the top molecular and cellular functions in our IPA analysis (Table 3).Endocytosis and exocytosis are crucial processes for neurotransmission [42] and regulated by SNARE and SM proteins (Sec1/Munc18-like proteins) [43].In particular, syntaxin-7 (member of the SNARE complex present on plasma membrane) and syntaxin binding protein (STXBP, also known as MUNC18) were found upregulated in DISC1silenced cells (Table 1).Other proteins that regulate the exocytic processes responsible for neuronal communication are Rab proteins [44], which catalyze SNARE complex assembly [45].In this study four different Rab proteins were found differentially expressed in DISC1-silenced cells (Table 1).

DISC1 silenced SH-SY5Y cells show impaired neurite outgrowth
To further test that silencing of DISC1 results in disruption of neural development, we performed a morphological study in SH-SY5Y cells in which DISC1 was silenced (Figure S4) [46].The absence of DISC1 in this cell line resulted in morphological changes (Figure 3).Thus, upon retinoic acid-induced differentiation, DISC1-silenced cells exhibited fewer and shorter neurites (Figure 3).(d, e) Frequency (percentile) of cells according to neurite length at 7 days (d) and 14 days (e) for each cell population; p<0.0001 control vs silenced 1 at 7 and 14 days, p<0.0001 control vs silenced 4 at 7 days, p<0.001 control vs silenced 4 at 14 days (Mann-Whitney U test).

Discussion
We have taken advantage of a well-established murine primary neuron DISC1 knock-down experimental system [23][24][25] to carry out an unbiased proteomic analysis and thus identify proteins whose expression is regulated by DISC1.
The results of our analysis highlight the importance of DISC1 both in neurodevelopment and synaptic regulation.Both functions have been already ascribed to DISC1; however, this study describes new important routes to explore, as the DISC1 regulation of CRMP family of proteins.This could be a powerful mechanism to further investigate considering the relevance this family of proteins has in the neurobiology of mental disease [33,47,48].
Furthermore, DISC1 knockdown resulted in a neurite outgrowth deficit in RA-treated SH-SY5Y cells.Previous studies have reported an impaired neurite outgrowth in cell models that overexpress mutant isoforms of DISC1 [11,49] and an increase of neurite outgrowth was seen in PC12 cells that overexpress DISC1 [50].Therefore our study reinforces the idea that the loss of function of DISC1 is critical for proper regulation of these neurodevelopmental processes.
At the same time, we have found that several proteins that participate in synaptic membrane trafficking and synapse formation are altered in DISC1 silenced neurons, such as syntaxin 7, MUNC-18, cadherin-13, and Rab proteins (Table 1), but we cannot conclude whether trafficking is up-or downregulated in our system.Previous studies have shown that DISC1 enhances the transport of synaptic vesicles, therefore we could expect that knocking down DISC1 expression produced an attenuated vesicle transport in primary cortical neurons [51].
Summarizing, our study shows that DISC1 is an important regulator of proteins that are directly involved both in neurodevelopment and in adult synaptic regulation, representing a unifier factor of two seemingly different categories.
Murine cortex and hippocampal primary neurons were prepared from 14-15 day embryos (see below ethical statement).Pregnant dams were killed by cervical dislocation in accordance with institutional guidelines for care and use of animals.The embryos were maintained and dissected in PBS Ca/Mg (Invitrogen) supplemented with 33 mM glucose.Pooled tissue was mechanically dissociated, treated with trypsin (Invitrogen) and DNaseI (Roche Applied Science, Mannheim, Germany) and resuspended in Neurobasal medium (Invitrogen) supplemented with 50X B27 (Invitrogen), 0.55g/100ml glucose (Sigma Aldrich), 42 mg/100 ml sodium bicarbonate (Sigma Aldrich), 1% P/S and 1% glutamine.The cells were plated on poly-D-lysine (Sigma Aldrich) coated Petri dishes.Cultures were maintained in serum free medium at 37ºC in 95% air/5% CO2.

Ethics statement.
Animal experiments were carried out in accordance with the European Union Council Directive 86/609/EEC, and were approved by the University of Santiago de Compostela Ethics Committee (protocol 15005AE / 12 / FUN 01 / PAT 05 / JRR2).

DISC1 silencing
For DISC1 knock-down in murine primary neurons, we chose a validated shRNA construct developed by the Sawa's group (DISC1 RNAi #1) that has been shown to specifically decrease the amount of DISC1 in cortical neural cell cultures [23][24][25].To that purpose, lentiviruses were produced by calcium phosphate triple co-transfection of shRNA (see Table S2 and Figure S2 in Supporting Information), VSVG and ΔR8.9 constructs into 293FT packaging cells.Virus-containing medium was collected 48h after transfection, and added (10 ml of lentiviral solution/ 3x10 of primary neurons at 7 DIV.The medium was changed 24 h after infection, and incubation continued for 72 h. In SH-SY5Y cells, DISC1 was silenced using commercial Mission® shRNA lentiviral transduction particles (Sigma Aldrich, reference NM_018662) containing two alternative PLKO.1-Puro-CMV shRNA plasmids (Figure S1 and Table S1 in Supporting Information).Mission® pLKO.1puronon-mammalian shRNA particles (reference: SHC002V) were used as control.

Proteomic studies
Primary neuron cell lysates were subjected to two-dimensional gel electrophoresis (2-DE).Protein quantitation was performed with the Coomassie plus protein reagent (Thermo Scientific, Asheville, NC).Five hundred micrograms of protein were loaded onto each gel to allow detection of low abundance proteins.Four gels per study group (DISC1 knock-down and control) were compared.Immobilized pH gradient (IPG) strips (4-7, 24 cm, GE Healthcare, Uppsala, Sweden) were rehydrated in the sample, and isoelectric focusing (IEF) was performed in a Multiphor (GE Healthcare) for 85 kVh at 17ºC.Following focusing, the IPG strips were immediately equilibrated for 15 min in 4 M urea, 2 M thiourea, 130 mM DTT, 50 mMTris pH 6.8, 2% w/v SDS, 30% v/v glycerol.Later, the strips were placed for 15 minutes in the same buffer, in which DTT was replaced by 4.5% iodoacetamide (Sigma Aldrich).The IPG strips were placed on top of the second dimension gels and embedded with 0.5% melted agarose.Proteins were separated in the second dimension by SDSpolyacrylamide gel electrophoresis (PAGE) on 10% gels at run conditions of 10ºC, 20 mA per gel for 1 h, followed by 40 mA per gel for 4 h by using an Ettan Dalt 6 system (GE Healthcare).Following electrophoresis, gels were fixed in 10% methanol/7% acetic acid for 1 hour, and stained overnight with Sypro Ruby fluorescent dye (Lonza, Switzerland).After staining, gels were washed for 1 hour in 10% methanol/7% acetic acid, and scanned in a Typhoon 9410 (GE Healthcare).

Differential image analysis
Image analysis was performed with the Ludesi REDFIN 3 Solo software (Ludesi, Malmö, Sweden).The integrated intensity of each of the spots was measured, and the background corrected and normalized.Differential expression of proteins was defined on the basis of ≥2-fold change between group averages and p<0.05.

Mass Spectrometric Analysis
Spots of interest were carefully excised and subjected to in-gel digestion with trypsin [26].Tryptic digests were analyzed using a 4800 MALDI-TOF/TOF analyzer (Applied Biosystems).Dried peptides were dissolved in 4 µL of 0.5% formic acid.Equal volumes (0.5 µl) of peptide and matrix solution, consisting of 3 mg alpha-cyano-4-hydroxycinnamic acid (α-CHCA) dissolved in 1 mL of 50% acetonitrile in 0.1% trifluoroacetic acid, were deposited using the thin layer method, onto a 384 Opti-TOF MALDI plate (Applied Biosystems).MS spectra were acquired in reflectron positive-ion mode with a Nd:YAG, 355 nm wavelength laser, averaging 1000 laser shots and using at least three trypsin autolysis peaks as internal calibration.All MS/MS spectra were performed by selecting the precursors with a relative resolution of 300 (FWHM) and metastable suppression.Automated analysis of mass data was achieved by using the 4000 Series Explorer Software V3.5.MS and MS/MS spectra data were combined through the GPS Explorer Software v3.6.Database search was performed with the Mascot v2.1 search tool (Matrix Science, London, UK) screening SwissProt (release 56.0).Searches were restricted to mouse taxonomy allowing carbamidomethyl cysteine as a fixed modification and oxidized methionine as potential variable modification.Both the precursor mass tolerance and the MS/MS tolerance were set at 30 ppm and 0.35 Da, respectively, allowing 1 missed tryptic cleavage site.All spectra and database results were manually inspected in detail using the above software.Protein scores greater than 56 were accepted as statistically significant (P< 0.05), considering positive the identification when protein score CI (confidence interval) was above 98%.In case of MS/MS spectra, total ion score CI was above 95%.
4.9.SDS-PAGE and Western Blotting 50 µg of protein was mixed with Laemmli sample buffer (BioRad), heated at 100 ºC for 10 minutes, spun, and the supernatant loaded on a 7.5% SDS-PAGE gel.Samples were subjected to electrophoresis and transferred to polyvinylidenedifluoride (PVDF) membranes (Millipore, Bedford, MA).The conditions of the electrophoresis were 200V, 1h.Electrophoresis was performed using a Mini-PROTEAN 3 cell electrophoresis system (BioRad).The transfer was performed in a Trans-blot SD semi-dry transfer cell (BioRad) using the following conditions: 0.8mA/cm2, 90 minutes.The PVDF membranes were blocked in 5% non-fat milk in PBS-0.1% Tween solution overnight at 4°C, then 4 washes of 5 minutes with PBS-0.1%Tween20 were performed, and the membrane was incubated with the primary antiserum (in 5% BSA in PBS-0.1%Tween20) for 1 hour at room temperature, washed again and incubated with the peroxidase-conjugated secondary antibody (in PBS-0.1% Tween20), and subjected to 4 washes of 5 minutes each with PBS-0.1%Tween20.Finally the membrane was incubated with the chemiluminescence solution Luminata Forte Western HRP substrate (Merck Millipore).To develop the membranes Hypercassette (GE Healthcare) and Amersham Hyperfilm ECL (GE Healthcare) were used.

2D Western blotting
The protocol used is the same that was used for the proteomic studies with minor differences: 7 cm pH 4-7 IPG strips were used and 50 µg of protein were loaded.8x8 cm commercial 4-12% Bis-TrisNuPAGE gels (Life Technologies) were used for the second dimension with MOPS running buffer (0.25 M Tris BASE, 1.92 M Glycine, 1% (w/v) SDS) were used.Second dimension was performed with constant voltage of 120V for 1h and 45 min.2-DE proteins were then transferred to a PVDF membrane.Immunodetections were performed as indicated above.

Ingenuity pathway
Ingenuity Pathway Analysis software (Ingenuity Systems, CA) was used to investigate interactions between all the identified proteins.Interactive pathways were generated to observe potential direct and indirect relations among the differentially expressed proteins.

Neurite outgrowth assays
Stable SH-SY5Y cell lines generated using TRCN0000118997 (Silenced 1), TRCN0000119000 (Silenced 4) and non-target shRNAs were cultured for 7 and 14 days in medium containing 10 µM retinoic acid (RA) (Sigma Aldrich).To analyse neurite outgrowth, images of live cells were taken under a microscope and processed using Image J software (http://rsb.info.nih.gov/ij).Cells with and without neurites longer than two cell bodies were counted in photomicrographs of the differentiated control and DISC1-silenced cells.

Immunocytochemistry of SH-SY5Y cells
Retinoic acid-differentiated cells were fixed in paraformaldehyde and immunostained for β3tubulin and nuclei were visualized using DAPI, as previously described [27].

Statistical analysis
One-way ANOVA was employed in the proteomic analysis to determine statistically significant differences between groups of samples.For each spot ID, ANOVA p-value was calculated using the quantified and normalized spots volumes for the matched spot in each of the images.Differential expression of proteins was defined on the basis of ≥2-fold change between group averages and p<0.05.
In the neurite outgrowth assay, three fields of up to 100 cells were analyzed for each condition and the experiment was performed twice.Statistical analysis was performed using a non-parametric unpaired Mann-Whitney U-test (two-tailed); results were considered significant with p < 0.05.

Conclusions
This study shows DISC1 regulates the expression of a number of proteins involved in neurodevelopment and synaptic function.Thus, DISC1 acts as a key regulator of two mechanisms that have been critically implicated in the development of mental disease.
Supplementary Materials: Supplementary materials can be found at www.mdpi.com/xxx/s1.

Figure 3 .
Figure 3. DISC1-silenced cells show morphological impairment in neurite outgrowth assays.Cells were treated with retinoic acid (RA) for 7 and 14 days and neurite length was measured using Image J. (a) Fluorescence images of SH-SY5Y cells expressing control and DISC1 shRNAs treated with RA for 7 days and immunostained for βIII-tubulin (red); nuclei were stained using DAPI (blue).(b, c) Average neurite length -/+ SD; * significantly different between control and DISC1-silenced cells (n > 200 for each cell line).(d,e) Frequency (percentile) of cells according to neurite length at 7 days (d) and 14 days (e) for each cell population; p<0.0001 control vs silenced 1 at 7 and 14 days, p<0.0001 control vs silenced 4 at 7 days, p<0.001 control vs silenced 4 at 14 days (Mann-Whitney U test).

Table 3 .
Ingenuity Top 10 molecular and cellular functions.

Table 4 .
Top 3 Ingenuity clusters for diseases and disorders