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Serine Protease Inhibitor SERPINE2 Reversibly Modulates Murine Sperm Capacitation

1
Department of Medical Research, Mackay Memorial Hospital, Tamsui District, New Taipei City 251, Taiwan
2
Mackay Junior College of Medicine, Nursing, and Management, Beitou District, Taipei City 112, Taiwan
3
Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei City 104, Taiwan
4
Mackay Medical College, Sanzhi District, New Taipei City 252, Taiwan
5
Department of Obstetrics and Gynecology, Taipei Medical University, Taipei City 110, Taiwan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(5), 1520; https://doi.org/10.3390/ijms19051520
Received: 2 May 2018 / Revised: 17 May 2018 / Accepted: 17 May 2018 / Published: 19 May 2018
(This article belongs to the Section Biochemistry)
SERPINE2 (serpin peptidase inhibitor, clade E, member 2), predominantly expressed in the seminal vesicle, can inhibit murine sperm capacitation, suggesting its role as a sperm decapacitation factor (DF). A characteristic of DF is its ability to reverse the capacitation process. Here, we investigated whether SERPINE2 can reversibly modulate sperm capacitation. Immunocytochemical staining revealed that SERPINE2 was bound onto both capacitated and uncapacitated sperm. It reversed the increase in BSA-induced sperm protein tyrosine phosphorylation levels. The effective dose and incubation time were found to be >0.1 mg/mL and >60 min, respectively. Calcium ion levels in the capacitated sperm were reduced to a level similar to that in uncapacitated sperm after 90 min of incubation with SERPINE2. In addition, the acrosome reaction of capacitated sperm was inhibited after 90 min of incubation with SERPINE2. Oviductal sperm was readily induced to undergo the acrosome reaction using the A23187 ionophore; however, the acrosome reaction was significantly reduced after incubation with SERPINE2 for 60 and 120 min. These findings suggested that SERPINE2 prevented as well as reversed sperm capacitation in vitro. It also prevented the acrosome reaction in in vivo-capacitated sperm isolated from the oviduct. Thus, SERPINE2 could reversibly modulate murine sperm capacitation. View Full-Text
Keywords: SERPINE2; sperm capacitation; decapacitation factor; acrosome reaction; seminal plasma SERPINE2; sperm capacitation; decapacitation factor; acrosome reaction; seminal plasma
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MDPI and ACS Style

Li, S.-H.; Hwu, Y.-M.; Lu, C.-H.; Lin, M.-H.; Yeh, L.-Y.; Lee, R.K.-K. Serine Protease Inhibitor SERPINE2 Reversibly Modulates Murine Sperm Capacitation. Int. J. Mol. Sci. 2018, 19, 1520. https://doi.org/10.3390/ijms19051520

AMA Style

Li S-H, Hwu Y-M, Lu C-H, Lin M-H, Yeh L-Y, Lee RK-K. Serine Protease Inhibitor SERPINE2 Reversibly Modulates Murine Sperm Capacitation. International Journal of Molecular Sciences. 2018; 19(5):1520. https://doi.org/10.3390/ijms19051520

Chicago/Turabian Style

Li, Sheng-Hsiang, Yuh-Ming Hwu, Chung-Hao Lu, Ming-Huei Lin, Ling-Yu Yeh, and Robert K.-K. Lee. 2018. "Serine Protease Inhibitor SERPINE2 Reversibly Modulates Murine Sperm Capacitation" International Journal of Molecular Sciences 19, no. 5: 1520. https://doi.org/10.3390/ijms19051520

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