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Int. J. Mol. Sci. 2018, 19(10), 3223; https://doi.org/10.3390/ijms19103223

Peanut Stunt Virus and Its Satellite RNA Trigger Changes in Phosphorylation in N. benthamiana Infected Plants at the Early Stage of the Infection

1
Institute of Plant Protection—National Research Institute, Department of Entomology, Animal Pests and Biotechnology, Władysława Węgorka 20, 60-318 Poznań, Poland
2
Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, 9052 Ghent, Belgium
3
VIB Center for Plant Systems Biology, Technologiepark 927, 9052 Ghent, Belgium
4
Department of Biomolecular Medicine, Ghent University, B-9000 Ghent, Belgium
5
VIB Center for Medical Biotechnology, B-9000 Ghent, Belgium
*
Author to whom correspondence should be addressed.
Received: 17 September 2018 / Revised: 11 October 2018 / Accepted: 16 October 2018 / Published: 18 October 2018
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Abstract

Signaling in host plants is an integral part of a successful infection by pathogenic RNA viruses. Therefore, identifying early signaling events in host plants that play an important role in establishing the infection process will help our understanding of the disease process. In this context, phosphorylation constitutes one of the most important post-translational protein modifications, regulating many cellular signaling processes. In this study, we aimed to identify the processes affected by infection with Peanut stunt virus (PSV) and its satellite RNA (satRNA) in Nicotiana benthamiana at the early stage of pathogenesis. To achieve this, we performed proteome and phosphoproteome analyses on plants treated with PSV and its satRNA. The analysis of the number of differentially phosphorylated proteins showed strong down-regulation in phosphorylation in virus-treated plants (without satRNA). Moreover, proteome analysis revealed more down-regulated proteins in PSV and satRNA-treated plants, which indicated a complex dependence between proteins and their modifications. Apart from changes in photosynthesis and carbon metabolism, which are usually observed in virus-infected plants, alterations in proteins involved in RNA synthesis, transport, and turnover were observed. As a whole, this is the first community (phospho)proteome resource upon infection of N. benthamiana with a cucumovirus and its satRNA and this resource constitutes a valuable data set for future studies. View Full-Text
Keywords: signaling; virus infection; Nicotiana benthamiana; Peanut stunt virus; plant-virus interactions; satellite RNA; phosphoproteome; phosphoproteomics; RNA turnover; phosphorylation signaling; virus infection; Nicotiana benthamiana; Peanut stunt virus; plant-virus interactions; satellite RNA; phosphoproteome; phosphoproteomics; RNA turnover; phosphorylation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Wrzesińska, B.; Dai Vu, L.; Gevaert, K.; De Smet, I.; Obrępalska-Stęplowska, A. Peanut Stunt Virus and Its Satellite RNA Trigger Changes in Phosphorylation in N. benthamiana Infected Plants at the Early Stage of the Infection. Int. J. Mol. Sci. 2018, 19, 3223.

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