Galectins in Cancer and Translational Medicine: From Bench to Bedside

<jats:p>n/a</jats:p>

galectin-mediated molecular interactions that are critical for tumors and can be potentially targetable with tailored neo-glycoproteins, glycomimetics, or other biological tools. It is likely that new avenues for cancer therapy will be explored in the near future [3][4][5][6][7].
As aforementioned, cancer does not represent the only playground in which galectins works. Several physiological processes seem to be regulated by galectins. Regulation of immune response, bone cell differentiation, and macrophages activation just represent some of the examples. A large number of papers recently published in the literature also support the notion that the biological relevance of galectins is not restricted to cancer. Galectins have been discovered to be functionally active in many different diseases, some of which deserve special attention for the possibility to target these molecules for a therapeutic intervention [8][9][10].
In this special issue, focused on galectins in translational medicine, a total of 20 interesting papers, 15 of which are represented by comprehensive reviews and 5 original research studies, have been finally considered. These contributions are clustered into 4 groups: (1) Structural and functional studies on galectins and ligands; (2) Galectins and cancer; (3) Galectins in non-neoplastic diseases; and (4) Miscellanea, as detailed in Table 1.

Cluster
Paper Reference

2) Galectins and Cancer
Proteomic identification of the galectin-1-involved molecular pathways in urinary bladder urothelial carcinoma Li C.F. et al. [13] Overall survival of ovarian cancer patients is determined by expression of galectins-8 and -9 Schulz H. et al. [14] Galectin-1, -3, and -7 are prognostic markers for survival of ovarian cancer patients Schulz H. et al. [15] Galectin-3 performance in histologic and cytologic assessment of thyroid nodules: a systematic review and meta-analysis Trimboli P. et al. [16] Galectins and carcinogenesis: their role in head and neck carcinoma and thyroid carcinomas Kindt N. et al. [17] Galectin-3: the impact on the clinical management of patients with thyroid nodules and future perspectives Bartolazzi A. et al. [18] Galectin-7 in epithelial homeostasis and carcinoma Advedissian T. et al. [19] TrkB-target galectin-1 impairs immune activation and radiation responses in neuroblastoma: implication for tumor therapy Batzke K. et al. [7] Role of galectins in multiple myeloma Storti P. et al. [20] Galectin targeted therapy in Oncology: Current knowledge and perspectives Wdowiak K. et al. [5] Role of galectins in tumors and in clinical immunotherapy Chou F.-C. et al. [6] Galectins as molecular targets for therapeutic intervention Dings R.P.M. et al. [4] Galectin-1 inhibitor OTX008 induces tumor vessel normalization and tumor growth inhibition in human head and neck squamous cell carcinoma models

Cluster Paper Reference (3) Galectins in Non-Neoplastic Diseases
Galectin-3: one molecule for an alphabet of diseases, from A to Z Sciacchitano S. et al. [22] Galectin-3 in atrial fibrillation: mechanisms and therapeutic implications Clementy N. et al. [8] Translational implication of galectin-9 in the pathogenesis and treatment of viral infection Lai J-H. et al. [9] (4) Miscellanea Role of galectin-3 in bone cell differentiation, bone pathophysiology and vascular osteogenesis Iacobini C. et al. [10] Galectin-12 in cellular differentiation, apoptosis and polarization Wan L. et al. [23] The first two papers [11,12] belong to the structural cluster. Research on galectin glycobiology has drawn much attention for the multifunctional features of these molecules, which can be considered potential targets for selective therapeutic interventions in many pathological contexts. The elegant report by Chan at al. [11] provides an in-depth review on galectin inhibitors, focusing on the structure-activity relationship with the attempt to clarify how these potential therapeutic tools interact with galectins. Structural and functional studies on the galectin-ligand interaction represent an important and necessary step for developing new tools tailored to bind galectins and to eventually block galectin-mediate functions. Bumba et al. [12] report a molecular model focused on the galectin-3 carbohydrate recognition domain. This specific study analyzes the mode and kinetics of galectin-3 bindings to a panel of multivalent neo-glycoproteins, which present complex poly-LacNAc-based oligosaccharide ligands on albumin scaffold. Significant differences in the binding kinetics are observed among the different glycoproteins. A tetrasaccharide capped with N,N -diacetillactosamine (LacdiNAc) showed the strongest ligand ability to galectin-3. This information may drive the development of neo-glycoproteins ingegnerized ad hoc to specifically interfere with galectin-3 mediated functions.
The cluster worded "Galectins and Cancer", as expected, is the larger one. The cluster includes several papers focused on cancer diagnosis, prognosis, and galectin-based therapeutic approaches [4][5][6][7][13][14][15][16][17][18][19][20][21]. The implication of galectins in tumor growth and progression, and more in general the impact that these molecules may have in oncology, is very well-known and has been extensively reviewed [3]. More specifically, for this paper collection, Li et al. [13] demonstrate the possibility to identify deregulated proteins, functionally related to galectin-1, by using an in vitro model of urinary bladder urothelial carcinoma and a proteomic approach. Modulation of these galectin-1 related molecules may have prognostic value for this tumor type. Similarly, a specific galectin signature, which can be used as prognostic marker, has been also identified in ovarian carcinoma [14,15].
Thyroid cancer represents the paradigmatic tumor model, in which experimental studies on galectins glycobiology, in particular on galectin-3, contributed greatly to improving cancer diagnosis. A validated galectin-3 test-method for the preoperative detection of thyroid cancer has been already translated in the clinical setting and is changing the clinical management of patients with thyroid nodules, reducing consistently unnecessary surgical procedures [24][25][26][27][28][29]. Three contributions in this specific field, showing different clinical experiences, are presented here [16][17][18]. A novel galectin-3-based immune-positron emission tomography (immune-PET) for imaging thyroid cacer in vivo, and its biological rationale is also presented and discussed [18,30,31]. One of the main functions of galectins in cancer is the regulation of tumor cell adhesion and migration. Modulation of these functions is likely relevant for tumor growth and progression. Galectin-7 is abnormally expressed in epithelial tumors and is involved in tumor progression and metastasis. Advedissian et al. [19] investigated the physiological function of this lectin in epithelial cells and demonstrated its aberrant expression in malignant epithelial counterparts. Studies on the functional role of galectins in neuroblastoma [7] and multiple myeloma [20], two relatively rare tumor conditions, are also presented in this special issue. Up-regulation of galectin-1 has been previously reported in aggressive neuroblastoma, the most common extracranial tumor of childhood. Galectin-1 is necessary for balancing immune response and angiogenic processes in physiological conditions, but cancer exploits these functions to escape from attacks of the host immune system and to better survive in hypoxic conditions. A strategic approach that combines radiotherapy and galectin-1 blockade is proposed and may be useful for reactivating an efficient tumor immune response [7]. Multiple myeloma are characterized by the tight adhesion between neoplastic plasma cells and a bone marrow microenvironment that allows for tumor cell survival and drug resistance. In this niche, neo-angiogenesis is induced as well as immunosuppression and osteolytic metastasis. In the review by Storti et al. [20], the authors analyze the expression profile of different galectin molecules and their functional activities in these specific tumors, with special focus on tumor progression, angiogenesis, and osteoclastogenesis. Several of the identified galectins may be attractive targets for multiple myeloma therapy. The expression of galectin-1 and -8 on tumor cells in fact, correlate with patients' survival.
Finally, three very interesting and comprehensive reviews exploring new potential therapeutic strategies for cancer treatment, based on galectin targeting, are considered in this book [4][5][6]. This paper represent a hot topic in oncology. Structural and functional studies on galectin-ligand interactions performed at molecular level, in fact, may produce important information for creating therapeutic interventions based on galectin-targeting. The proposed therapeutic approaches are supported by a strong biological rationale. New therapeutic strategies for cancer may involve the use of specific galectin inhibitors, such as competitive carbohydrates, small glycomimetic molecules, and monoclonal antibodies to specific galectins, that can be used alone or in combination with other therapeutic options.
An interesting issue is also represented by the fact that tumor-derived galectins may have bifunctional effects on tumor cells and immune cells. This has been extensively studied for galectin-1, galectin-3, and galectin-9 in different cancer types. In the review by Chou et al. [6], this issue is specifically discussed.
A short communication by Koonce et al. [21] reports the biological effects of a non-peptidic galectin-1 allosteric inhibitor, worded OTX008 and able to induce tumor vessel normalization and tumor growth inhibition in squamous cell carcinoma models. These effects are related to the improved tumor oxygenation registered after treatment with such a galectin-1 inhibitor.
This condition allows a more permissive tumor microenvironment status for improved radiation or chemotherapeutic treatment.
The cluster entitled "Galectins in Non-Neoplastic Diseases" includes three interesting reviews. The first by Sciacchitano and co-authors [22] is focused on galectin-3. The authors report in an elegant and original way a large number of studies published so far on the role of galectin-3 in different diseases, listed in alphabetical order. This original contribution provides the base for further investigations in each specific medical field.
It has been previously reported that galectin-3 is required for TGF-β mediated myofibroblast activation and matrix production. Increased galectin-3 expression seems to be a common feature in tissue fibrosis. Disruption of the galectin-3 gene blocks myofibroblast activation and procollagen I expression, in an experimental model of liver fibrosis in vitro and in vivo [23]. Clementy et al. described the role of galectin-3 in the pathogenesis of atrial fibrillation [8]. Considering the fact that galectin-3 is a well-recognized biomarker of fibrosis and tissue remodeling, the authors discuss the mechanisms of such an electrical alteration and its therapeutic implications.
A very interesting review published by Lai and co-authors [9] is focused on the translational implication of galectin-9 in the pathogenesis and treatment of viral infection. The interaction between galectin-9 and the ligand Tim-3 triggers signaling events that regulate immune response.  has been reported to be over-expressed in a variety of target cells infected by different viruses, among which include HCV, HBV, HSV, influenza virus, dengue virus, and HIV. The biological significance of galectin-9 expression in virus infected cells and its functional implications in regulating virus specific immune-response also represent important fields of research to be pursued. Understanding the biological role of galectin-9 in this specific context may help to identify better methods for monitoring and treating viral infections. The last cluster of contributions includes the work by Iacobini et al. [10], where they reported original data on galectin-3's biological role in all stages of bone biology, from development to remodeling. The regulatory role of galectin-3 in inflammatory bone and joint disorders is also discussed, together with the possibility to target galectin-3 for therapeutic purposes.
The special review issue on the recently discovered galectin-12 and its functional role in regulating important physiological mechanisms, like cellular differentiation, apoptosis, and macrophage polarization to M2 subtype [32], closes on galectins and translational medicine. Although galectin biology is attracting the interest of many scientists operating in different experimental contexts and clinical fields, from developmental biology to clinical oncology, many aspects concerning galectins are still incompletely understood and need further investigation. Among these, some deserve mention: elucidation of galectin-mediated intracellular mechanisms; galectin-dependent signaling; structural aspects of galectin functions (i.e., oligomerization, galectin-derived extracellular lattice); and the biological and functional significance of specific "galectin signatures" in benign and malignant tissue as well. Addressing these issues will open the way to new therapeutic strategies for different human diseases, stimulating research for developing glycomimetics, neo-glycoproteins, and/or monoclonal antibodies directed to specifically modulate or block the functions of these fascinating molecules.
Overall 20 important contributions published in this special issue illustrate recent advances in the field of Galectins, which may have potential clinical implications in cancer but also in other clinical contexts. It seems to me that an interesting chapter of translational glycobiology is on the horizon.
I would like to thank all the authors who contributed to this special issue and I remain hopeful that the increasing knowledge in the field will require, in the very near future, a new and more extensive effort for preparing the second volume entitled "Galectin in cancer and translational medicine II".