Secondary Metabolites in Ramalina terebrata Detected by UHPLC/ESI/MS/MS and Identification of Parietin as Tau Protein Inhibitor

Liquid chromatography coupled with mass spectrometry is an outstanding methodology for fast analysis of phenolic compounds in biological samples. Twenty two compounds were quickly and accurately identified in the methanolic extract of the Antarctic lichen Ramalina terebrata for the first time using ultra high pressure liquid chromatography coupled with photodiode array detector and high resolution mass spectrometry (UHPLC-PDA-Q/Orbitrap/MS/MS). In addition, the extract and the four compounds isolated from this species were tested for the inhibitory activity of tau protein aggregation, which is a protein involved in Alzheimer’s disease (AD). All compounds showed null activity with the exception of parietin, which it was able to inhibit aggregation process of tau in a concentration range between 3 µg/mL (10 µM) to 28 µg/mL (100 µM). In addition, we show how parietin interact with tau 306VQIVYK311 hexapeptide inside of the microtubule binding domain (4R) with the help of molecular docking experiments. Finally, the constituents present in the methanolic extract could possibly contribute to the established anti-aggregation activity for this extract and this in-depth analysis of the chemical composition of R. terebrata could guide further research into its medicinal properties and potential uses.


Introduction
Lichens are symbiotic associations between heterotrophic fungi and algae and/or cyanobacteria.A peculiarity of lichen is its remarkable ability to tolerate extreme atmospheric conditions such as low temperatures in polar zones including the Arctic and Antarctic regions; these regions are very cold; the coldest temperature ever known on earth (´129 ˝F) was recorded in Antarctica.These environmental conditions are responsible for the diversity of secondary metabolites produced in lichens [1][2][3][4][5].Lichen substances are mainly synthesized via poly-malonyl, shikimate, and mevalonic acid pathway, which have afforded several interesting and unique phenolic structures such as dibenzofurans, depsides, depsidones, depsones, quinones and pulvinic acid derivatives [1][2][3][4][5][6][7].
Alzheimer´s disease is the most common form of dementia.There are two main proteins involved, β-amyloid protein and microtubule-associated tau protein.Both proteins are characterized by the deposition of plaques and neurofibrillary tangles, respectively [8].Physiologically, tau protein is involved in axonal transport and microtubule stability.However, once tau is hyper-phosphorylated, it detached from microtubules and starts to form aggregates in soma and dendrites of neuron cells [9].Tau is an unfolded protein whose structure has two fibril-forming motif, 275 VQIINK 280 and 306 VQIVYK 311 [10].Besides, in order to form the fibrillar structure of tau, is required the addition of polyanions such as heparin which suggest an important role of electrostatic interaction to form both fibrils and aggregates [11].These two motifs are within the microtubule binding domain of tau and are prone to forming a cross β structure [12,13].
The determination of amyloid-like structure reveals the presence of moieties involved in β sheets pair formation [14], this "steric zipper" is formed from short self-complementary segments of the amyloid [15].Thus, this formation is a central part of proto-filaments, whereas the rest of the protein remains unfolded outside of the main axis [16].The Q-exactive focus is a newly released hybrid high resolution mass spectrometer used for metabolomics analysis including pesticides, herbicides, drugs, antibiotics, small peptides and several other organic molecules [17][18][19][20][21].The hyphenated Q-exactive focus instrument combines ultra high pressure liquid chromatography coupled with photodiode array detector (UHPLC-PDA) with an orbital trap and a high-resolution collision cell, which allows high resolution MS n fragments [17][18][19][20][21].
On the other hand, molecular docking is a computational method used for the prediction of ligand-receptor interactions and is an important tool for rational drug design [22].Today, molecular docking is the most important theoretical method to determine the orientation of the ligands inside a binding site.Particularly, the challenges of molecular docking are the following: the prediction of ligands proper orientation, the prediction of the binding energies and the prediction of novel, effective drugs by using the structural knowledge obtained from the models [22][23][24].Several examples using these computational methods have been already reported [23][24][25][26][27][28].
The present work describes the UHPLC chromatographic fingerprints plus the isolation of the main secondary metabolites together with the tau aggregation inhibitory activity of methanolic extract of Ramalina terebrata.Hook.and Taylor.Based on the reported activity of fulvic acid [29] we decided to investigate parietin, in order to demonstrate its capacity to inhibit tau protein aggregation.Moreover, we modeled the structure of the complex between parietin and fibril-forming motif VQIVYK of tau using docking experiments.Hence, we are able to demonstrate that parietin is able to create hydrogen bonds (HB) with lysine residues.
Alzheimer´s disease is the most common form of dementia.There are two main proteins involved, β-amyloid protein and microtubule-associated tau protein.Both proteins are characterized by the deposition of plaques and neurofibrillary tangles, respectively [8].Physiologically, tau protein is involved in axonal transport and microtubule stability.However, once tau is hyper-phosphorylated, it detached from microtubules and starts to form aggregates in soma and dendrites of neuron cells [9].Tau is an unfolded protein whose structure has two fibril-forming motif, 275 VQIINK 280 and 306 VQIVYK 311 [10].Besides, in order to form the fibrillar structure of tau, is required the addition of polyanions such as heparin which suggest an important role of electrostatic interaction to form both fibrils and aggregates [11].These two motifs are within the microtubule binding domain of tau and are prone to forming a cross β structure [12,13].
The determination of amyloid-like structure reveals the presence of moieties involved in β sheets pair formation [14], this "steric zipper" is formed from short self-complementary segments of the amyloid [15].Thus, this formation is a central part of proto-filaments, whereas the rest of the protein remains unfolded outside of the main axis [16].The Q-exactive focus is a newly released hybrid high resolution mass spectrometer used for metabolomics analysis including pesticides, herbicides, drugs, antibiotics, small peptides and several other organic molecules [17][18][19][20][21].The hyphenated Q-exactive focus instrument combines ultra high pressure liquid chromatography coupled with photodiode array detector (UHPLC-PDA) with an orbital trap and a high-resolution collision cell, which allows high resolution MS n fragments [17][18][19][20][21].
On the other hand, molecular docking is a computational method used for the prediction of ligand-receptor interactions and is an important tool for rational drug design [22].Today, molecular docking is the most important theoretical method to determine the orientation of the ligands inside a binding site.Particularly, the challenges of molecular docking are the following: the prediction of ligands proper orientation, the prediction of the binding energies and the prediction of novel, effective drugs by using the structural knowledge obtained from the models [22][23][24].Several examples using these computational methods have been already reported [23][24][25][26][27][28].
The present work describes the UHPLC chromatographic fingerprints plus the isolation of the main secondary metabolites together with the tau aggregation inhibitory activity of methanolic extract of Ramalina terebrata.Hook.and Taylor.Based on the reported activity of fulvic acid [29] we decided to investigate parietin, in order to demonstrate its capacity to inhibit tau protein aggregation.Moreover, we modeled the structure of the complex between parietin and fibril-forming motif VQIVYK of tau using docking experiments.Hence, we are able to demonstrate that parietin is able to create hydrogen bonds (HB) with lysine residues.

Results and Discussion
From the methanolic extract, the following compounds were isolated: parietin 1, usnic acid 2, atraric acid 3 and inositol 4 (Figure 1) using a combination of chromatographic techniques [30].Initially, the methanolic extract was screened by Thioflavin T (ThT) at concentration ranging from 100 to 1000 µg/mL and the results reveled that at 1000 µg/mL the inhibitory activity against aggregation process of tau protein was almost complete (Figure 2).Therefore, we have performed the isolation of lichen substances from this extract for further testing of this activity.All isolated compounds were tested in ThT fluorescence assay, since it has been demonstrated that ThT is able to bind to fibrils from both synthetic and biological sources [31,32].None of these compounds were active to prevent tau aggregation with the exception of parietin (Figure 2).
Initially, the methanolic extract was screened by Thioflavin T (ThT) at concentration ranging from 100 to 1000 µg/mL and the results reveled that at 1000 µg/mL the inhibitory activity against aggregation process of tau protein was almost complete (Figure 2).Therefore, we have performed the isolation of lichen substances from this extract for further testing of this activity.All isolated compounds were tested in ThT fluorescence assay, since it has been demonstrated that ThT is able to bind to fibrils from both synthetic and biological sources [31,32].None of these compounds were active to prevent tau aggregation with the exception of parietin (Figure 2).Parietin, an orange anthraquinone pigment, is a metabolite very common in the family Teloschistaceae.Several biological activities for this compound have been summarized [1,4].Besides, it is noteworthy to mention that parietin isolated from Xanthoria parietina (Linnaeus) Theodor Fries showed antibacterial activity against S. aureus (ATCC and clinical isolate strains), antifungal activity towards Rhizoctonia solani, Botrytis cinerea and Candida albicans.In contrast, parietin did not show any effect regarding anticancer and antiproliferative activity [2][3][4].Gauslaa and Ustvedt reported that parietin may reduce the effect of UV radiations [2][3][4].However, there is no information regarding its capacity to inhibit tau aggregation process.Furthermore, according to our knowledge, there is no published data on anti-aggregation properties of lichen compounds.
The aggregation assay was performed using fragment 4R of the protein tau as positive control.Once we tested parietin by ThT, our results showed that parietin was able to inhibit aggregation process of tau in a concentration range between 3 µg/mL (10 µM) to 28 µg/mL (100 µM) (Figure 2) showing a dose-response effect.The inhibitory effect of parietin at 28 µg/mL was by 75%.Some compounds have been described for their anti-aggregating effect against over either amyloid-β or tau protein [33][34][35].In addition, anthraquinones compounds such as daunorubicin, adriamycin and emodin inhibit tau aggregation and also diminish paired helical filaments in cells [36].A previous report has shown that emodin, an anthraquinone related to parietin has profound effects on aggregation process of tau protein [36], this difference could be because parietin has a methoxy group at C-3 position instead of hydroxyl group of emodin.
There are previous evidences that negatively charged molecules such as orange-G bind specifically to the lysine residues of tau fibril-forming motifs VQIVYK [37].Considering that parietin has groups with a negative charge density, we proposed that its activity against aggregation process of tau is due to molecular interactions with fibril-forming motifs.In the complex between orange-G and VQIVYK, the fragment of tau has a β-sheet form with the dye binding between two sheets.Considering this information, we constructed the possible tridimensional structure of parietin in interaction with the tau VQIVYK motif using docking.Since we do not have information about the preferred protonation state of parietin forming the complex, we tested the protonation states P1 and P2 described in Materials and Methods section.We docked parietin structures inside a cavity formed Parietin, an orange anthraquinone pigment, is a metabolite very common in the family Teloschistaceae.Several biological activities for this compound have been summarized [1,4].Besides, it is noteworthy to mention that parietin isolated from Xanthoria parietina (Linnaeus) Theodor Fries showed antibacterial activity against S. aureus (ATCC and clinical isolate strains), antifungal activity towards Rhizoctonia solani, Botrytis cinerea and Candida albicans.In contrast, parietin did not show any effect regarding anticancer and antiproliferative activity [2][3][4].Gauslaa and Ustvedt reported that parietin may reduce the effect of UV radiations [2][3][4].However, there is no information regarding its capacity to inhibit tau aggregation process.Furthermore, according to our knowledge, there is no published data on anti-aggregation properties of lichen compounds.
The aggregation assay was performed using fragment 4R of the protein tau as positive control.Once we tested parietin by ThT, our results showed that parietin was able to inhibit aggregation process of tau in a concentration range between 3 µg/mL (10 µM) to 28 µg/mL (100 µM) (Figure 2) showing a dose-response effect.The inhibitory effect of parietin at 28 µg/mL was by 75%.Some compounds have been described for their anti-aggregating effect against over either amyloid-β or tau protein [33][34][35].In addition, anthraquinones compounds such as daunorubicin, adriamycin and emodin inhibit tau aggregation and also diminish paired helical filaments in cells [36].A previous report has shown that emodin, an anthraquinone related to parietin has profound effects on aggregation process of tau protein [36], this difference could be because parietin has a methoxy group at C-3 position instead of hydroxyl group of emodin.
There are previous evidences that negatively charged molecules such as orange-G bind specifically to the lysine residues of tau fibril-forming motifs VQIVYK [37].Considering that parietin has groups with a negative charge density, we proposed that its activity against aggregation process of tau is due to molecular interactions with fibril-forming motifs.In the complex between orange-G and VQIVYK, the fragment of tau has a β-sheet form with the dye binding between two sheets.Considering this information, we constructed the possible tridimensional structure of parietin in interaction with the tau VQIVYK motif using docking.Since we do not have information about the preferred protonation state of parietin forming the complex, we tested the protonation states P1 and P2 described in Materials and Methods section.We docked parietin structures inside a cavity formed between two steric zippers (model A) and on the surface of one steric zipper (model B); models A and B (Figure 3). between two steric zippers (model A) and on the surface of one steric zipper (model B); models A and B (Figure 3).Docking results for parietin in protonation states P1 and P2 inside the model A, shown in Figure 3A.The obtained models suggest that parietin could serve as inhibitor of aggregation by binding between steric zippers preventing higher-order β-sheet interactions as orange-G [37].The phenolic groups and the oxygen of methoxy substituent form hydrogen bond (HB) interactions with different VQIVYK lysine side chains.At once, the methyl group of the methoxy substituent has hydrophobic interactions with the VYK valine.It is noteworthy that the results are comparable for both protonation states of parietin.
The docking results for parietin in protonation states P1 and P2 inside the model B are shown in Figure 3B.The models obtained show that both protonation states have similar orientations forming HB interactions with lysine and glutamine side chains.Phenolic groups and carbonyl groups at position 9 of the anthracene-9,10-dione scaffold have HB interactions with glutamine side chains, while the oxygen of methoxy substituent and carbonyl at position 10 of the anthracene-9,10-dione scaffold have HB interactions with lysine side chains.At the same time, methyl groups orient near VYK valine forming hydrophobic interactions.
Regarding the HPLC fingerprint of the methanolic extract, 22 compounds were identified for the first time in the methanolic extract of R. terebrata with the help of their characteristic UV-Vis spectra and high-resolution mass spectrometry [38,39].All compounds were detected in negative mode using UHPLC-Q/Orbitrap/ESI/MS/MS (Table 1).Peak 22 was identified as parietin (molecular anion at m/z 283.0601).Peak 21 was identified as usnic acid, which showed a [M ´H] ´peak at m/z 343.0803.Major diagnostic daughter MS ions of usnic acid were [ ´. Peak 8 could be tentatively identified as 4-O-dimethylbaemycesic acid (m/z 359.0756) which produced a MS 2 ion at m/z 302.0417.Ten tetrahydroxy fatty acids (peak 1-3, 5, 7, 9-11 and 13-14) and three pentahydroxy fatty acids (peak 4, 6 and 12) were tentatively identified as the polihydroxy fatty acids reported by Huneck [30].
Regarding the lichen-derived substances, usimine C has showed anti-proliferative activity on human dermal fibroblasts [41].Usnic acid, usimine A-C and ramalin isolated from Antarctic lichen R. terebrata, displayed activity against B. subtilis but no activity against S. aureus, where the values of MIC ranged from 1-26 µg/mL [40].Ramalin showed to be a more powerful antioxidant than butylhydroxytoluene (BHT), vitamin E, Trolox and ascorbic acid as well as a very good antiinflammatory agent [43].Sekikaic acid and homosekikaic acid showed IC 50 values of 0.082 mg/mL and 0.276 mg/mL at the linoleic acid peroxidation assay, demonstrating that these compounds are promising antioxidants.The antioxidant activity of atranorin, protolichesterinic acid, usnic acid, 2-hydroxy-4-methoxy-6-propylbenzoic acid, homosekikaic acid, sekikaic acid, 2,4-dihydroxy-6-propylbenzoic acid and 2,4-dihydroxy-3,6-dimethylbenzoate isolated from the hexane extract from R. roesleri were assessed by the DPPH method.Among the compounds, the best antioxidant activity was exhibited by sekikaic acid, followed by homosekikaic acid.Usnic acid has demostrated to reduce the production of Junin virus in infected Vero cells in a dependent dose manner (EC 50 9.9 µM), these results indicate that usnic acid present antiviral activity.Also, usnic acid is not genotoxic and cytotoxic towards human lymphocyte A549, promyelocytic leukemia HL-60, and ovarian carcinoma A2780.D-protolichesterinic acid and nephrosterinic acid showed activity against Ehrlich carcinoma.D-protolichesterinic acid and lobaric acid are considered 5-and 12-lipoxygenase inhibitors [40].

Collection and Identification of Lichen Species
Ramalina terebrata was collected in "Peninsula Fildes" Antarctic region, Chile during March, 2014.Lichens were carefully removed from the rocks by abrasion.A voucher specimens were deposited at the Extreme Natural Product laboratory, Universidad de Chile whose reference numbers is RT-010414.R. terebrata (50 g) were dried, powdered and extracted with methanol (3 ˆ0.5 L) to afford, after evaporation of the solvent under vacuum, 450 mg of a dark gum.

Tau Protein Production
Tau fragment 4R (htau 244-372 ) was amplified by using the plasmid for htau40 as a template.The PCR sequence was subcloned into pET-28a vector (Novagen, Madinson, WI, USA) to produce a His-tagged protein.The recombinant fragment 4RMBD was expressed in Escherichia coli strain BL21 (DE3) as described [29].LB medium containing kanamycin was inoculated with a stationary overnight culture.Bacterial culture was grown at 37 ˝C to OD 600 of 0.5-0.6 and protein expression was induced by addition of 1 mM IPTG for 4 h; and cells were pelleted and sonicated.Recombinant tau was purified via a succession of Ni-Sepharose chromatography (equilibrated in 20 mM NaH 2 PO 4 , 500 mM NaCl, and 20 mM imidazole, pH 7.4, elution with buffer 200 mM imidazole) and side exclusion chromatography coupled to HPLC in a Proteema 100 column (PSS, Mainz, Germany) with buffer 50 mM NaH 2 PO 4 , 300 mM NaCl, pH 6.5.The purity of the protein was verified on a Coomassie Brilliant Blue-stained SDS-polyacrylamide gel.The protein was concentrated and stored at ´80 ˝C until use.The concentration of purified 4RMBD was determined using the extinction coefficient at 280 nm (1520 M ´1¨cm ´1).

Thioflavin T Assay
The ThT fluorescence assay adopted here was modified from the reported by Pickhardt et al. [36].Briefly, to examine the inhibition of tau aggregation, the total volume of the reaction mixture was 100 µL, which included 20 µM 4RMBD, 5 µM heparin in 100 mM sodium acetate, pH 7.0 with parietin at different concentrations.After 20 h of incubation at 37 ˝C, addition of 100 µL of a 25 µM solution of ThT was made and incubation continued for 1 h at room temperature prior to fluorescence reading.Then, the fluorescence was measured in a Biotek H1 multi-mode reader (Biotek Instruments, Winooski, VT, USA) with an excitation wavelength at 440 nm and emission wavelength of 485 nm in a 96-well plate.Each experiment was replicated at least three times and background fluorescence was subtracted. 3.5.UHPLC-Q/Orbitrap/MS/MS

Instrument
A Thermo Scientific Dionex Ultimate 3000 UHPLC system equipped with a quaternary Series RS pump and a Thermo Scientific Dionex Ultimate 3000 Series TCC-3000RS column compartments with a Thermo Fisher Scientific Ultimate 3000 Series WPS-3000RS autosampler and a rapid separations PDA detector controlled by Chromeleon 7.2 Software (Thermo Fisher Scientific, Bremen, Germany) hyphenated with a Thermo high resolution Q Exactive focus mass spectrometer were used for analysis.The chromatographic system was coupled to the MS with a Heated Electrospray Ionization Source II (HESI II).Nitrogen (purity > 99.999%) obtained from a Genius NM32LA nitrogen generator (Peak Scientific, Billerica, MA, USA) was employed as both the collision and damping gas.Mass calibration for Orbitrap was performed once a week, in both negative and positive modes, to ensure a working mass accuracy lowers than or equal to 5 ppm.Cafeine and N-butylamine (Sigma Aldrich, Saint Louis, MO, USA) were the calibration standards for positive ions and buspirone hydrochloride, sodium dodecyl sulfate, and taurocholic acid sodium salt were used to calibrate the mass spectrometer.These compounds were dissolved in a mixture of acetic acid, acetonitrile, water and methanol (Merck Darmstadt, Hesse, Germany) and were infused using a Chemyx Fusion 100 syringe pump.XCalibur 2.3 software and Trace Finder 3.2 (Thermo Fisher Scientific, San Jose, CA, USA) were used for UHPLC control and data processing, respectively.Q Exactive 2.0 SP 2 from Thermo Fisher Scientific was used to control the mass spectrometer.

MS Parameters
The HESI parameters were optimized as follows: sheath gas flow rate 75 units; aux.gas unit flow rate 20; capillary temperature 400 ˝C; aux gas heater temperature 500 ˝C; spray voltage 2500 V (for ESI ´); and S lens RF level 30.Full scan data in both positive and negative was acquired at a resolving power of 70,000 FWHM (full width half maximum) at m/z 200.For the compounds of interest, a scan range of m/z 100-1000 was chosen; the automatic gain control (AGC) was set at 3 ˆ10 6 and the injection time was set to 200 ms.Scan-rate was set at 2 scans¨s ´1.External calibration was performed using a calibration solution in positive and negative modes before each sample series.In addition to the full scan acquisition method, for confirmations purposes, a targeted MS/MS analysis was performed using the mass inclusion list and expected retention times of the target analytes, with a 30 s time window, with the Orbitrap spectrometer operating both in positive and negative mode at 17,500 FWHM (m/z 200).The AGC target was set to 2 ˆ10 5 , with the maximum injection time of 20 ms.The precursor ions are filtered by the quadrupole which operates at an isolation window of m/z 2. The fore vacuum, high vacuum and ultrahigh vacuum were maintained at approximately 2 mbar, from 105 and below 1010 mbar, respectively.Collision energy (HCD cell) was operated at 30 kV.Detection was based on calculated exact mass and on retention time of target compounds, as shown in Table 1.The mass tolerance window was set to 5 ppm for the two analysis modes.

Molecular Modeling
The current structural information about tau protein is very limited; however, our results suggest that parietin could interact with tau fibril-forming motifs VQIINK (PHF6*), particularly with lysine residues.The coordinates of the hexapeptide VQIVYK were extracted from the X-ray crystal structure of tau VQIVYK segment complexed with orange-G (Protein Data Bank code 3OVL) [47,48].The fiber structure was prepared using the VQIVYK coordinates.Zinc atoms were removed since zinc solution was not used in our study.We prepared two fiber structure models A and B: A contains 24 units of hexapeptide and B contains 12 units of the hexapeptide.In model A, each member of a pair of VQIVYK β-sheets is shifted relative to the other, without the dry interface in the typical steric zipper structure, forming a cylindrical cavity [47,48]; docking was executed inside the cavity.The model B just contains the half of peptides of model A, and B does not contain a cavity; therefore, the docking was executed at surface.Using models A and B, we evaluated the interactions between the parietin and the VQIVYK fiber structure using these models considering the presence and absence of a cavity.
For docking, we considered two protonation states of parietin: P1 with neutral phenolic groups, and P2 with deprotonated phenolic groups.These two extreme cases allow studying the effect of the protonation state of molecular interactions with hexapeptide VQIVYK.Both models of parietin (P1 and P2) were sketched using Maestro's molecular editor.Docking was performed using Glide method [47,48].A grid box of 15 Å ˆ10 Å ˆ10 Å covered the whole cavity in model A, and whole surface in model B. Docking parameters were used as previously reported, a Glide extra-precision (XP) modes were explored during search.Docking hierarchy begins with systematic conformational expansion of ligand followed by placement on receptor site.Then, minimization of the ligand in the receptor field was carried out using the OPLS-AA [49] force field with a distance-dependent dielectric of 2.0.Afterwards, the lowest energy poses were subjected to a Monte Carlo procedure that samples the nearby torsional minima.The best pose for a given ligand was determined by the Emodel score, while different compounds were ranked using GlideScore [50].Docking poses were analyzed by examining their relative total energy score.The most energetically favorable conformations were selected as best poses.

Statistical Analysis
Results of statistical analysis were expressed as the mean ˘SEM.In all experiments, statistical differences between treatments and their respective control were determined by Paired t-test.Significance level was set at p < 0.05.All statistical analyses were developed using GraphPad Prism 6 software (H.Motulsky, San Diego, CA, USA).

Conclusions
Finally, our results demonstrate that parietin has a moderate activity against the aggregation process of tau protein, while the methanolic extract of Ramalina terebrata had also activity that could be attributed to sinergistic effects of the compounds detected in the extract.On the other hand, docking experiments suggest that parietin is bound to fiber-forming segment VQIVYK of tau mediated mainly by HBs interactions with the lysine residues.Based on UHPLC-Q/Orbitrap/ESI/MS/MS, 22 compounds were identified in the methanolic extract of the Antarctic lichen Ramalina terebrata.Finally, in-depth analysis of the chemical composition of R. terebrata could guide further research into its medicinal properties and potential uses.

Figure 2 .
Figure 2. Tau aggregation process inhibited by both Ramalina extract and parietin.Black and grey bars represent positive control (aggregation) and inhibition respectively.A paired samples-t-test was conducted in order to compare control (aggregation) and tau inhibitors (Ramalina extract and parietin).There was a significant difference for both Ramalina extract t (4) = 25, p < 0.05 and parietin t (4) = 3.223, p < 0.05 (data are represented as Mean ± SEM).

Figure 2 .
Figure 2. Tau aggregation process inhibited by both Ramalina extract and parietin.Black and grey bars represent positive control (aggregation) and inhibition respectively.A paired samples-t-test was conducted in order to compare control (aggregation) and tau inhibitors (Ramalina extract and parietin).There was a significant difference for both Ramalina extract t (4) = 25, p < 0.05 and parietin t (4) = 3.223, p < 0.05 (data are represented as Mean ˘SEM).

Figure 3 .
Figure 3. (A) Docking of parietin in protonation state P1 in the interface of two poly-306 VQIVYK 311 hexapeptide zippers (a,c-left).Hydrogen bounds (HBs) between parietin and lysine residues are indicted as broken lines in the perpendicular view (b,d-right); (B) Docking of parietin in protonation state P2 in the interface of two poly-306 VQIVYK 311 hexapeptide zippers (a,c-left).Hydrogen bounds (HBs) between parietin and lysine residues are indicated as broken lines in the perpendicular view (b,d-right).

Figure 3 .
Figure 3. (A) Docking of parietin in protonation state P1 in the interface of two poly-306 VQIVYK 311 hexapeptide zippers (a,c-left).Hydrogen bounds (HBs) between parietin and lysine residues are indicted as broken lines in the perpendicular view (b,d-right); (B) Docking of parietin in protonation state P2 in the interface of two poly-306 VQIVYK 311 hexapeptide zippers (a,c-left).Hydrogen bounds (HBs) between parietin and lysine residues are indicated as broken lines in the perpendicular view (b,d-right).
0583, 259.0612 and 231.0663 amu, respectively).Peak 20 was identified as lobaric acid (molecular anion at m/z 455.1712).The fragmentation of peak 20 also produced ions at 411.1808 [M ´H ´CO 2 ] ´, 367.1909 [M ´H ´2CO 2 ] ´, 352.1675 [M ´H ´2CO 2 ´CH 3 ] ´, and 296.1049 [M ´H ´2CO 2 ´C5 H 11 ] ´confirming this depsidone.Peak 19 and 17 had the same [M ´H] ´ion at m/z 375.1070 with different retention time based on UHPLC at 22.04 and 23.65 min, which were tentatively identified as placodiolic acid or pseudoplacodiolic acid, respectively.Peak 18 with a [M ´H] ´ion at m/z 527.2290 was identified as arthoniaic acid, and peak 16 as gyrophoric acid, which was identified by spiking experiments with an authentic standard.Peak 15 with a [M ´H] ´ion at m/z 497.1065 was identified as 3-hydroxyumbilicaric acid.Main daughter ion of peak 15 was at m/z 317.0652 [M ´H ´C9 H 8 O 4 ]